key: cord-0026323-u79q8x25 authors: Xing, Yueyi; Tian, Zibin; Jiang, Yueping; Guan, Ge; Niu, Qinghui; Sun, Xueguo; Han, Rongshuang; Jing, Xue title: A practical nomogram based on systemic inflammatory markers for predicting portal vein thrombosis in patients with liver cirrhosis date: 2022-01-21 journal: nan DOI: 10.1080/07853890.2022.2028893 sha: 614cad5bc2ba041af91887e5d90274b957f4a835 doc_id: 26323 cord_uid: u79q8x25 INTRODUCTION: Immunothrombosis has recently been used to describe the responses/mechanisms in thrombosis. Systemic inflammatory markers are prognostic markers for a variety of thrombotic conditions; however, their potential value in predicting portal vein thrombosis (PVT) is unknown. This study aimed to establish an easy-to-use nomogram based on systemic inflammatory markers to predict portal vein thrombosis (PVT) in patients with liver cirrhosis. PATIENTS AND METHODS: This retrospective study included 478 patients with cirrhosis between January 2013 and January 2021. Reputed systemic inflammatory markers (systemic immune-inflammation index [SII], neutrophil-to-lymphocyte ratio [NLR], monocyte-to-lymphocyte ratio [MLR], and platelet-to-lymphocyte ratio (PLR)) were measured, and the clinical data were recorded. The independent risk factors for PVT were determined using univariate analyses and multivariate logistic regression analyses, and a nomogram to predict the occurrence of PVT was established. The concordance index, receiver operating characteristic curves, and calibration plots were used to evaluate the performance of the model. RESULTS: A total of 239 patients with PVT and 239 patients without PVT were selected. In the univariate analysis, high SII, NLR, PLR, and MLR were significantly associated with PVT. NLR and PLR were independent risk factors for PVT (P < 0.05) by multivariate analysis. The nomogram had good predictive efficiency for PVT in patients with cirrhosis, with an area under the receiver operating characteristic (AUROC) curves of 0.891 (95% CI 0.862–0.919) and the calibration curves fit as well, indicating that the nomogram had good clinical application value. CONCLUSIONS: PVT in patients with cirrhosis is associated with increased levels of systemic inflammatory markers. We successfully developed a practical nomogram based on NLR and PLR to accurately predict PVT, which is a practical method helping clinicians rapidly and conveniently diagnose and guide the treatment of PVT in patients with cirrhosis. KEY MESSAGES: The present study is the first report on a nomogram based on systemic inflammatory markers in patients with portal vein thrombosis (PVT). The nomogram had good predictive efficiency and a good clinical application value for predicting PVT in patients with cirrhosis. Portal vein thrombosis (PVT) is defined as thrombosis within the portal vein trunk and intrahepatic portal branches, with or without the involvement of the mesenteric and splenic veins [1] . PVT is a frequent and serious complication in patients with liver cirrhosis (LC). The prevalence of PVT in patients with LC ranges from 0.6% to 26% [2] , and the 2-year survival of patients with PVT is reduced by 55% owing to hepatic dysfunction [3] . Remarkably, most patients are asymptomatic at the time of PVT diagnosis [4] . Therefore, there is an urgent requirement for more rapid and easy-to-use diagnostic techniques for the detection of PVT. In recent years, words like "thromboinflammation," "immunothrombosis," and "immunohemostasis" have been used to describe the responses/mechanisms that are involved in both thrombosis and inflammation [5] . The systemic immune-inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and platelet-to-lymphocyte ratio (PLR) are reported predictors of venous thrombosis, such as deep vein thrombosis [6, 7] , cerebral venous thrombosis [8] , and thrombosis associated with cancers [9, 10] . However, the impact of systemic inflammatory markers on the progression of PVT remains unclear. Our study aimed to establish a practical nomogram based on systemic inflammatory markers to predict PVT, which can help clinicians rapidly and quickly diagnose and guide the treatment of patients with liver cirrhosis. This retrospective study was conducted on patients with LC admitted to the Affiliated Hospital of Qingdao University from January 2013 to January 2021. The diagnosis of LC was based on clinical, laboratory, and radiological analyses, and/or liver biopsies. PVT was diagnosed according to the consensus for the management of PVT in LC (2020, Shanghai) [1] . The inclusion criteria were as follows: (I) age !18 years, (II) Doppler ultrasound was the first-choice imaging modality; however, enhanced computed tomography or magnetic resonance imaging could also be used for confirmation at the time of admission to our hospital, and (III) patients with PVT on imaging examination but with insufficient evidence for the diagnosis of cirrhosis, hepatic vein pressure gradient measurement, and liver biopsy. Patients with primary or secondary hepatic malignant tumours, other malignant tumours, haematologic diseases, Budd-Chiari syndrome, non-cirrhotic PVT, inflammatory diseases, and other severe diseases were excluded. All data were collected in our hospital, and all serum indicators were obtained at the time of the first diagnosis of PVT. A study flow chart is illustrated in Figure 1 . Data included the demographic status, aetiology of the liver disease, clinical laboratory tests, and imaging characteristics. Clinical laboratory tests included Ddimer, activated partial thromboplastin time (APTT), prothrombin time (PT), antithrombin III, international normalised ratio (INR), thrombin time (TT), albumin (Alb), serum creatinine, haemoglobin, platelet count, white blood cell count, neutrophil count, lymphocyte count, monocyte count, a model for end-stage liver disease (MELD) score, and Child-Pugh score. Imaging characteristics included splenic vein diameter, splenic vein velocity, portal vein diameter, and portal vein velocity. Data were collected at the time of diagnosis of LC with PVT from the hospital medical records. The systemic inflammatory markers were calculated as follows: The SII was calculated as the platelet count  neutrophil count/lymphocyte count (10 9 /L). The NLR was calculated as the neutrophil count/ lymphocyte count. The MLR was calculated by dividing the monocyte count by the lymphocyte count. The PLR was calculated by dividing the platelet count by the lymphocyte count. The Institutional Review Board of the Affiliated Hospital of Qingdao University (QYFYW2LL26362) approved the use of medical record data for this study. Categorical variables are expressed as frequency and percentage, and the significance was determined using v 2 or Fisher's exact test. Quantitative variables are expressed as mean ± standard deviation, and the significance was determined using the Student's t-test. Non-normally distributed variables are expressed as a median and interquartile range, and the significance was determined using the Mann-Whitney U test. The quantitative variables were converted to categorical variables based on the cut-off values. Multivariate logistic regression analyses were performed to identify the independent risk of PVT. The discrimination of the nomogram was measured by calculating the area under the receiver operating characteristic (AUROC) and the concordance index. The model calibration was determined using the Hosmer-Lemeshow technique and calibration curve. Internal validation was performed using bootstrap resampling. We fit the model repeatedly in 1000 bootstrap samples and evaluated its performance on the original samples. Differences were considered significant at P <0 .05. Analyses were performed with the SPSS 24.0 statistical package and R version 3.6.1. The risk of bias and reporting quality for this study were assessed against Transparent Reporting of a Multivariable Prediction Model for Individual Prognosis or Diagnosis and the Prediction model Risk of Bias Assessment Tool. After screening by the inclusion and exclusion criteria, 239 patients of LC with PVT and 239 patients of LC without PVT were included in the two cohorts. The baseline characteristics of the patients are shown in Table 1 . There were no significant differences in the age, sex, or history of smoking and alcohol consumption between the patients with or without PVT. The study participants had a mean age of 55.4 ± 9.85 years in the PVT cohort and 54.4 ± 9.13 years in the non-PVT cohort. Cirrhosis caused by hepatitis or alcohol abuse was not significantly different between the two cohorts. However, there were more patients (40/239; 17%) in the non-PVT cohort than in the PVT cohort (9/ 239; 4%) wherein LC was caused by autoimmune disease. Diabetes mellitus (P¼ 0.02) was more common in patients with PVT. Child-Pugh levels were significantly different between the patients with and without PVT; however, the MELD scores were not significantly different between the two cohorts. Endoscopic ligation therapy, endoscopic sclerotherapy, and splenectomy were more commonly performed in patients with PVT (allP< 0.001). The best cut-off values for SII, NLR, MLR, and PLR were 268.9 (area under the curve, 0.612; P< 0.001), 3.14 (area under the curve, 0.596; P< 0.001), 0.37 (area under the curve, 0.662; P< 0.001), and 103.4 (area under the curve, 0.628; P < 0.001), respectively ( Figure 2 ). As shown in Table 2 , the systemic inflammatory markers (SII, NLR, MLR, and PLR) were higher in the PVT group than in the non-PVT group (all P< 0.001) and thus were predictive factors for PVT. In addition, APTT (v 2 ¼ 5.741, P¼0.019), TT (v 2 ¼ 9.622, P¼ 0.002), D-dimer (v 2 ¼ 163.664, P< 0.001), splenic vein diameter (v 2 ¼ 27.547,P< 0.001), and splenic vein velocity (v 2 ¼ 14.75, P< 0.001) were significantly different between PVT group and non-PVT group. The significant factors in the univariate analysis in Table 1 , 2 were included in the multivariate logistic regression analysis (Table 3) . NLR ! 3.14, PLR ! 103.35, endoscopic ligation, D-dimer, splenectomy, splenic vein diameter, and absence of autoimmune liver disease were independently associated with PVT. A nomogram was established based on the NLR ! 3.14, PLR ! 103.35, endoscopic ligation, D-dimer, splenectomy, splenic vein diameter, and absence of autoimmune liver disease, which were used to predict the probability of PVT by adding the score corresponding to each factor and projecting the total score to the bottom scale ( Figure 3 ). The AUROC curve of the model was 0.891 (95% CI: 0.862-0.919) (Figure 4) . The Hosmer-Lemeshow test revealed that the v 2 was 2.491 (P¼ 0.928). The concordance index was 0.891. The calibration plots for bootstrap resampling validation showed good consistency ( Figure 5 ). In recent years, words like "thromboinflammation," "immunothrombosis," and "immunohemostasis" have been used to describe responses/mechanisms that are involved in both thrombosis and inflammation [5] . Some studies have proposed a hypothesis based on the systemic activation of the coagulation system in response to the dysregulation of the inflammatory markers [11] . This study found that the NLR and PLR were independently associated with PVT. If NLR is !3.14 or PLR !103, the risk of PVT will be 2.89 times or 2.23 times in patients without PVT, respectively. Compared to other prognostic nomograms of PVT [12, 13] , the model of this study cites inflammation indexes as predictors, while NLR and PLR can be easily obtained from routine blood indicators. This nomogram based on systemic inflammatory markers is practical, quantitative, and intuitive, and thus is convenient for use by clinicians. It has been reported that patients with cirrhosis have a hypercoagulable state resulting from the increased levels of factor VIII and decreased levels of protein C, which may result in the development of PVT [14] . In this study, the patients with PVT had a significantly higher serum level of D-dimer than patients without PVT, which further provided evidence of hypercoagulation, as reported by others [15, 16] . The role of diabetes in venous thrombosis remains controversial. Patients with diabetes mellitus have a hypercoagulable state that may increase their risk for thromboembolism, such as microvascular occlusive disease. However, some studies showed no association between diabetes mellitus and the incidence of venous thrombosis [17, 18] . This was not an independent risk factor for PVT in this study. The reported incidence of PVT in patients with cirrhosis following splenectomy was 24-29% [19] ; however, the mechanism of the PVT is still under investigation, which may be related to a hypercoagulable state, platelet activation, modification in endothelial cells, or hemodynamic changes [20, 21] . Studies have reported that splenectomy may predispose patients with or without cirrhosis to PVT [22] . Splenic vein diameter has been reported as a risk factor for post-splenectomy PVT in several reports [23] [24] [25] . The high hemodynamic status of the spleen affects the peripheral platelet count. In general, the larger thediameter and flow of the splenic vein, the lower the white blood cell count [26] . We also found that an increased splenic vein diameter was a risk factor for PVT in LC. A decrease in the blood flow in the portal vein could be associated with the development of PVT following splenectomy, although no significant difference in the reduction of the portal flow was found between patients with and without PVT in the current study. One meta-analysis demonstrated that endoscopic variceal ligation for primary prophylaxis of oesophageal varices in adults with cirrhosis reduced the incidence of variceal haemorrhage and mortality compared with no therapy [27] . Exposure to surgery or invasive procedures potentially increases the risk of developing bacterial infections in patients with LC, which may affect the markers of inflammation. Lin [29] . Endoscopic ligation was found to be an independent risk factor for PVT. Endoscopic sclerotherapy has also been reported as a risk factor for PVT [15] ; however, it was not a statistically significant factor in this study. Particular attention should be paid to PVT following endoscopic treatment. Inflammation is one of the most typical features of chronic autoimmune liver disease [30] . Patients without LC with autoimmune diseases, such as antiphospholipid disease, have an increased risk for PVT [31] ; however, there is no correlation between autoimmune liver disease and PVT in patients with LC. The results of this study suggest that patients with cirrhosis, but no evidence of autoimmune liver disease, still have an increased tendency for the development of PVT. Our study had several strengths and limitations: This was a monocentric and retrospective study design. Therefore, large-scale multicenter studies are required for more representative samples with higher statistical power. PVT in patients with LC is associated with increased levels of systemic inflammatory markers. We successfully developed a practical nomogram based on NLR and PLR to accurately predict PVT, which may help clinicians rapidly and easily diagnose and further guide the treatment of PVT in patients with LC. We declare that there are no conflicts of interest regarding the publication of this paper. Yueyi Xing made the major contribution to performing the experiments and drafted the manuscript. Zibin Tian obtained institutional review board approval. Yueping Jiang contributed to the data acquisition. Ge Guan, Qinghui Niu, Xueguo Sun and Rongshuang Han assisted in statistical analysis. 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