key: cord-0025838-r02f20di
authors: Bhavsar, Amit; Aris, Emmanuel; Harrington, Lauriane; Simeone, Jason C; Ramond, Anna; Lambrelli, Dimitra; Papi, Alberto; Boulet, Louis-Philippe; Meszaros, Kinga; Jamet, Nicolas; Sergerie, Yan; Mukherjee, Piyali
title: Burden of Pertussis in Individuals with a Diagnosis of Asthma: A Retrospective Database Study in England
date: 2022-01-11
journal: J Asthma Allergy
DOI: 10.2147/jaa.s335960
sha: 207e2b8f45ab6a4a44fe5fe027362619bfa1b477
doc_id: 25838
cord_uid: r02f20di

PURPOSE: The impact of pertussis in individuals with asthma is not fully understood. We estimated the incidence, health care resource utilization (HCRU), and direct medical costs (DMC) of pertussis in patients with asthma. PATIENTS AND METHODS: In this retrospective cohort study, the incidence rate of pertussis (identified using diagnostic codes) among individuals aged ≥50 years with an asthma diagnosis was assessed during 2009–2018 using Clinical Practice Research Datalink and Hospital Episode Statistics databases. HCRU and DMC were compared – between patients with diagnoses of asthma and pertussis (asthma+/pertussis+) and propensity score-matched patients with a diagnosis of asthma without pertussis (asthma+/pertussis–) – in the months around the pertussis diagnosis (–6 to +11). RESULTS: Among 687,105 individuals, 346 had a reported pertussis event (incidence rate: 9.6/100,000 person-years of follow-up; 95% confidence interval: 8.6–10.7). HCRU and DMC were assessed among 314 asthma+/pertussis+ patients and 1256 matched asthma+/pertussis– controls. Baseline HCRU was similar in both cohorts, but increases were observed in the asthma+/pertussis+ cohort from –6 to –1 month before to 2–5 months after diagnosis. Rates of accident and emergency visits, general practitioner (GP)/nurse visits, and GP prescriptions were 4.3-, 3.1-, and 1.3-fold, respectively, in the asthma+/pertussis+ vs asthma+/pertussis– cohorts during the month before diagnosis; GP/nurse visit rates were 2.0- and 1.2-fold during 0–2 and 2–5 months after diagnosis, respectively (all p<0.001). DMC was 1.9- and 1.6-fold during the month before and 2 months from diagnosis, respectively, in the asthma+/pertussis+ vs asthma+/pertussis– cohorts (both p<0.001). During months –1 to +11, DMC in the asthma+/pertussis+ cohort was £370 higher than in the asthma+/pertussis– controls. CONCLUSION: A pertussis diagnosis among adults aged ≥50 years with asthma resulted in significant increases in HCRU and DMC across several months around diagnosis, suggesting lengthy diagnosis times and highlighting the need for prevention strategies.

What Did the Researchers Do and Find?

• Among individuals with a diagnosis of asthma who were aged ≥50 years, there were approximately 10 pertussis cases diagnosed per 100,000 people per year (in England during 2009-2018), with a maximum of 21 per 100,000 in 2012. • In the month before the pertussis diagnosis, patients with diagnoses of asthma and pertussis (asthma+/pertussis+) had more general practitioner/nurse visits and accident and emergency visits and received more prescriptions (particularly for asthma medications and antibiotics) than patients with a diagnosis of asthma without pertussis (asthma +/pertussis-). • During the 5 months from the pertussis diagnosis, asthma +/pertussis+ patients continued to have more general practitioner/nurse visits than asthma+/pertussis-patients. • This increase in health care use around a pertussis diagnosis resulted in significant increases in health care costs. • The overall health care costs associated with an episode of pertussis among individuals aged ≥50 years with a diagnosis of asthma were £370.

What do These Results Mean?

Pertussis (also known as whooping cough) is a highly contagious respiratory infection commonly caused by Bordetella pertussis. 1 It is characterized by paroxysmal cough, posttussive vomiting, inspiratory whooping, and absence of fever in adults. 2 It can also result in complications such as urinary incontinence, sinusitis, and pneumonia, especially among older adults. 3 Pertussis is often perceived as a childhood illness, 4 but only around one third of pertussis notifications in England in 2019 were in children aged 0-14 years, with another third in individuals aged 15-44 years, and the remaining third in adults aged ≥45 years. 5 The overall incidence of laboratory confirmed pertussis cases across all age groups in England in 2019 was 7 per 100,000 population 6 (approximately 5 notifications per 100,000 among those aged ≥45 years), 5, 7 but seroprevalence studies from Europe indicate that the true rate of pertussis infection could be hundreds or even thousands of times higher than the reported incidence. [8] [9] [10] Data from patients with chronic obstructive pulmonary disease (COPD) in England also support this under-recognition, as the rate of diagnosed pertussis was approximately 5 per 100,000 in 2013, 11 but a seroprevalence study in the same year indicated that 5.8% of COPD patients had antibody levels indicative of exposure to B. pertussis in the past year. 12 Pertussis is particularly under-recognized or misdiagnosed in adults, 13, 14 possibly because health care providers often view pertussis as a childhood disease, 15 instead suspecting acute bronchitis or simply "cough", 16 also because adults can be asymptomatic 17 or present with mild or atypical symptoms. 18 Asthma affects approximately 7% of adults in the United Kingdom (UK) based on data from 2016. 19 Individuals with asthma appear to be at increased risk of pertussis 18, 20, 21 and hospitalization after a pertussis diagnosis. 21 For example, in a United States (US) database study, the incidence of diagnosed pertussis was 27 vs 7 per 100,000 person-years (PY) among people aged ≥11 years with asthma vs matched people without asthma or COPD. 21 In an Australian cohort study of adults aged ≥45 years, pertussis incidences were approximately 160 vs 90 per 100,000 PY for those with vs without asthma. 20 In a US study, hospitalized pertussis patients were approximately three times more likely to have asthma than the overall population. 22 In a Canadian study, patients with asthma and pertussis had a longer period of paroxysmal coughing and more nights of disturbed sleep than people with pertussis without asthma. 3 Most also had to increase their use of bronchodilators. 3 Patients with asthma or COPD have also been reported to incur higher health care resource utilization (HCRU) and direct medical costs (DMC) 21 than those with pertussis alone. Among adults with COPD, a diagnosis of pertussis has been associated with higher HCRU and DMC, 11 but the additional burden of asthma and pertussis compared to asthma alone has not yet been studied.

The objectives of the current study were to: (1) estimate the incidence rate of reported pertussis among individuals aged ≥50 years with a history of asthma in England (during 2009-2018) and (2) compare HCRU and DMC among patients with diagnoses of asthma and pertussis (asthma+/pertussis+) and those with a diagnosis of asthma without pertussis (asthma+/pertussis-). along with data from the following linked datasets: Hospital Episode Statistics (HES) Admitted Patient Care, HES Outpatient, HES Accident and Emergency (A&E), and 2015 Index of Multiple Deprivation (IMD). Further details are provided in Supplementary Text 1.

The study protocol received CPRD ethics approval via the Independent Scientific Advisory Committee on March 4, 2020 (protocol number 20_043). The study complied with all applicable laws regarding subject privacy.

This observational study (GSK study identifier HO-18-19690) used two different designs. Firstly, a retrospective cohort design was used to assess the reported incidence rate of pertussis among individuals aged ≥50 years with a diagnosis of asthma. Secondly, a propensity score-matched cohort analysis was used to evaluate HCRU and DMC among two cohorts of patients: asthma+/pertussis+ vs asthma+/pertussis-. HCRU and DMC were estimated during various timeframes (see Figure 1 ) on either side of the index date, which was the date of first pertussis diagnosis for the asthma+/pertussis+ cohort. For the asthma+/pertussis-comparators, the index date was the date of pertussis diagnosis of the corresponding pertussis case as determined by propensity score matching.

For the estimation of pertussis incidence rate, we included patients aged ≥50 years with a diagnosis of asthma (≥1 of the diagnosis codes detailed in Supplementary Tables 1-3) who were registered with general practices in the CPRD GOLD and Aurum datasets during January 2009 through November 2018. Patients with a prior history of pertussis (see Supplementary Tables 4-8) were excluded, and the  incident pertussis diagnosis (identified using the diagnosis  codes listed in Supplementary Tables 6-8 ) had to be on or after the earliest asthma diagnosis. For GOLD practices, their up-to-standard date (showing continuous recording of patient data) had to be within the study period.

For the evaluation of HCRU and DMC, patients aged ≥50 years with a diagnosis of asthma who had a pertussis diagnosis during January 2009 through August 2018 (to allow for 3 months of follow-up) were propensity score matched (1:4) to individuals aged ≥50 years with a diagnosis of asthma who had no pertussis diagnosis during January 2009 through November 2018 or end of follow-up. Patients were required to have continuous CPRD enrolment for ≥18 months before the index date.

Baseline characteristics, which are only reported for the matched cohorts for the HCRU/DMC analysis, include:

• At the index date: age, sex, ethnicity, year, season, and IMD (based on location of residence) • Most recent record during the 18 months before the index date: body mass index and forced expiratory volume in 1 second percentage predicted • During 18 months before index: obesity, asthma-related hospitalizations (defined as overnight hospitalizations with a primary diagnosis code of asthma, with admissions <14 days apart considered as the same event) and treatment corresponding to Global Initiative for Asthma (GINA) 2020 treatment step 4/5 (i.e., a prescription for 

Incident pertussis was identified using diagnosis codes (Supplementary Tables 6-8 ). Pertussis incidence rates among patients with a diagnosis of asthma aged ≥50 years were estimated for the whole 10-year study period (overall and for each 5-year age group) and per calendar year (all patients).

HCRU was estimated and compared between patients aged ≥50 years in the asthma+/pertussis+ vs asthma+/pertussiscohorts. HCRU included all-cause: general practitioner (GP)/nurse visits, GP prescriptions, outpatient specialist visits, A&E visits, and hospitalizations. HCRU was measured monthly (per 30-day period) and during various time intervals (see Figure 1 ) from -6 months to +11 months around the index date.

Annualized DMC (reported in 2019 £) -from the perspective of the National Health Service (NHS) -were estimated during the time intervals shown in Figure 1 based on the identified HCRU multiplied by the relevant unit costs. Unit costs for GP, nurse, and outpatient specialist visits were derived from the 2019 Unit Costs of Health and Social Care published by the Personal Social Services Research Unit from the University of Kent. 25 Unit costs for the five most commonly prescribed medications (in the asthma +/pertussis+ cohort; during each time interval shown in Figure 1 ) were derived from the September 2019 NHS Drug Tariff. 26 Unit costs for the five most common clinical assessments (e.g., blood pressure, weight, laboratory tests) and A&E costs were derived from the 2018/2019 National Schedule of NHS Reference Costs. 27 As hospitalizations are financed using Healthcare Resource Groups (HRGs) in the UK, 2018/2019 HRG4+ Reference Costs Grouper software 28, 29 was used to assign an HRG code for each hospitalization. 30 Costs were then derived by matching the assigned HRG code and hospitalization type 30 with those in the 2018/2019 National Schedule of NHS Reference Costs. 27 Further costing details can be found in Supplementary Text 2.

All statistical programming was performed using SAS software version 9.4. Statistical methods pertaining to each part of the study are provided below.

Pertussis incidence rates (overall, by year, and by age group) were estimated by dividing the number of incident pertussis cases by the number of PY at risk. Individuals contributed to the person-time at risk from the latest of: study start date, start date of continuous registration, asthma diagnosis, the middle of the year in which they turned 50 (full birth dates were not available), or the date at which the practice had an "up-to-standard" flag (CPRD GOLD only). Ninety-five percent confidence intervals (95% CIs) were calculated using the "exact" method by means of the Poisson distribution. 31 Propensity Score Matching Asthma+/pertussis+ patients aged ≥50 years were propensity score matched (1:4) to asthma+/pertussis-patients aged ≥50 years. Baseline variables used to calculate the propensity score (based on the literature 21,23,32-34 and expert consultation) were: age, sex, environmental/socioeconomic deprivation (IMD), smoking status, DMC, severe asthma (treatment corresponding to GINA 2020 treatment step 4/5), asthmarelated hospitalizations, and presence of hypertension, hyperlipidemia, depression, anxiety, and COPD. Further details can be found in Supplementary Text 3.

Baseline characteristics after propensity score matching are described as means and standard deviations for continuous variables, and as numbers and percentages for https://doi.org/10.2147/JAA.S335960

Journal of Asthma and Allergy 2022:15 38 categorical variables. Standardized mean differences (SMDs) were used to assess match quality and balance in baseline characteristics for continuous and binary variables, with SMDs <0.20 considered to indicate balanced values. For multilevel categorical variables where the SMD could not be estimated, p-values were used to estimate the balance between matched cohorts. Further details can be found in Supplementary Text 3.

HCRU events were included from the index date until the earliest of: the end of the study period, disenrollment from the database, or death. All-cause HCRU are described as rates per 100 PY. The rates and 95% CIs were estimated by fitting a negative binomial model. As the results showed non-normality, the Wilcoxon rank-sum test was used to compare HCRU between the asthma+/pertussis+ and asthma+/pertussis-cohorts. To account for multiple testing across time points and endpoints, the threshold for statistical significance was set at p<0.001. All p-values <0.05 but ≥0.001 were considered suggestive of a trend.

DMC were compared between the asthma+/pertussis+ and asthma+/pertussis-cohorts at baseline and around the index date using the Wilcoxon rank-sum test, with the same significance thresholds as for HCRU.

Excess DMC among asthma+/pertussis+ vs asthma +/pertussis-comparators during months -1 to +11 around index were estimated using a generalized linear model (GLM) analysis. The model used a log link, and normal, gamma, and Tweedie distributions were tested. Total DMC in primary and secondary care was used as the dependent variable, with log-years as an offset; no additional covariates were included in the model. The threshold for statistical significance for the GLM result was set at p<0.05.

Among 687,105 patients aged ≥50 years with a diagnosis of asthma, who contributed 3.6 million PY of follow-up (PYFU), 346 had a pertussis diagnosis. The overall incidence rate of pertussis was 9.6 per 100,000 PYFU (95% CI 8.6-10.7) and was highest in 2012 (20.9; 95% CI 16.4-26.2) ( Figure 2 ). The pertussis incidence rate was highest among those aged 50-54 years (15.7 per 100,000 PYFU; 95% CI 12.9-18.9) and appeared to decrease with increasing age ( Figure 2 ).

The 314 asthma+/pertussis+ patients who met the inclusion criteria for this part of the study were matched (1:4) with 1256 asthma+/pertussis-individuals. The baseline characteristics of the asthma+/pertussis+ and asthma 

Bhavsar et al +/pertussis-cohorts were generally well balanced, apart from a higher prevalence of chronic/persistent cough in the asthma+/pertussis+ cohort (Table 1) . DMC during baseline were also well matched in the two cohorts (Table 1) . During months -6 to -1 before the index date, the rates of GP/nurse and A&E visits were significantly higher (1.3and 1.7-fold, respectively) in the asthma+/pertussis+ cohort than in the asthma+/pertussis-cohort ( Figure 3A ). During the 2 months from the index date, GP/ nurse visits (2.0-fold [2.0 vs 1.0 per patient per month]) and clinical assessments (1.2-fold) remained significantly higher in the asthma+/pertussis+ cohort, with a trend towards an increase in hospitalizations (1.4-fold) ( Table 2 and Figure 3B ). During months 2 to 5 after the index date, GP/nurse visits remained significantly elevated in the asthma+/pertussis+ cohort (1.2-fold), along with a trend towards an increase in outpatient specialist visits (1.2-fold) ( Table 2 ). At 5 to 11 months after the index date, HCRU was similar in both cohorts, apart from a trend for more GP/nurse visits in the asthma+/pertussis+ cohort ( Table 2) .

Considerably more patients in the asthma+/pertussis+ vs asthma+/pertussis-cohorts had ≥1 A&E visit during months -6 to -1 before index (17.8% vs 10.3%; Supplementary Table 9 ). In the month before index, many more patients in the asthma+/pertussis+ cohort had ≥1 GP/nurse visit (87.6% vs 48.1%), ≥1 GP prescription (86.3% vs 64.3%), ≥1 clinical assessment (69.1% vs 27.9%), and ≥1 A&E visit (8.9% vs 2.2%; Supplementary Table 9 ).

There were also some significant differences in the use of the five most common prescriptions (in the asthma +/pertussis+ cohort) during the different time periods. During months -6 to -1 before the index date, prescription rates for oral prednisolone 5 mg (98 vs 42 per 100 PY) and amoxicillin 500 mg (78 vs 30 per 100 PY) (both p<0.001) were higher in the asthma+/pertussis+ vs asthma +/pertussis-cohorts, with 24.5% of patients in the asthma +/pertussis+ cohort receiving amoxicillin (Table 3) . During the month before index, prescription rates were higher in the asthma+/pertussis+ vs asthma+/pertussiscohort for oral prednisolone 5 mg (314 vs 41 per 100 PY), clarithromycin 500 mg (225 vs 10 per 100 PY), salbutamol 100 µg/dose (194 vs 109 per 100 PY), and omeprazole 20 mg (260 vs 150 per 100 PY) (all p<0.001). During the 2 months from index, clarithromycin prescriptions remained significantly elevated (83 vs 12 per 100 PY; p<0.001) ( Table 3) . Clarithromycin was prescribed to 16.9% vs 0.8% and 14.0% vs 1.8% of patients in the asthma+/pertussis+ vs asthma+/pertussis-cohorts during the month before and the 2 months from index, respectively (Table 3) .

There were also significant differences in the five most common outpatient services visited (in the asthma+/per-tussis+ cohort) between the asthma+/pertussis+ and asthma+/pertussis-cohorts, namely diagnostic imaging (58 vs 11 per 100 PY) and ear, nose, and throat (43 vs 9 per 100 PY) during the month before index; and thoracic medicine during months 2 to 5 (39 vs 12 per 100 PY) and 5 to 11 (23 vs 9 per 100 PY) after index (all p<0.001, data not shown). No significant differences were observed at other timepoints, although there were trends towards increases in cardiology (during months -6 to -1, 2 to 5, and 5 to 11), thoracic medicine (months -1 to 0 and 0 to 2), ear, nose, and throat (months 0 to 2), and diagnostic imaging (months 2 to 5) (data not shown).

Descriptive monthly HCRU data are presented in Figure 4 . In the asthma+/pertussis+ cohort, GP/nurse visits started to increase around 3 months before index (pertussis diagnosis), peaked at 3.4 visits per patient during the month before index (vs 1.1 visits per patient in the asthma+/pertussis-cohort), and remained elevated until around 3 months after index ( Figure 4A ). GP prescriptions also started to increase around 3 months before index and peaked at 5.3 per patient during the month before index (vs 4.0 per patient in the asthma+/pertussis-cohort) ( Figure 4B ). Outpatient specialist visits varied by month in both cohorts, with no obvious increase around the pertussis diagnosis ( Figure 4C ). However, A&E visits started to increase from 4 months before index and peaked 1 month before index, at which time they were 4.3-fold those in the asthma+/pertussis-cohort ( Figure 4D ), while hospitalizations peaked during months -2 to -1 ( Figure 4E ). Of note, HCRU in the asthma+/pertussiscohort remained relatively stable throughout the study. 

the asthma+/pertussis+ vs asthma+/pertussis-cohorts by the Wilcoxon rank-sum test (p<0.001) although mean values were numerically similar. During the month before the index date, mean total annualized DMC per patient were £1362 higher in the asthma+/pertussis+ cohort (p<0.001), predominantly due to a £1014 increase for GP/nurse visits, but also a £145 increase for A&E visits. During the 2 months from the index date, mean total annualized DMC per patient were £833 higher in the asthma+/pertussis+ cohort (p<0.001), mainly due to £448 and £335 increases for GP/ nurse visits and hospitalizations, respectively. Annualized DMC remained significantly elevated in the asthma+/pertus-sis+ cohort during months 2 to 5, but beyond 5 months after the index date, they were statistically similar in the two cohorts. The total annualized DMC per patient in the asthma+/pertussis+ vs asthma+/pertussis-cohorts during months -1 to +11 months were £1825 vs £1404 (p<0.001).

The Tweedie model distribution was selected for the regression analysis as it was the only one to converge due to the skewness of the data and existence of zero values. 35 The total estimated DMC increase per patient in the asthma+/pertussis+ vs asthma+/pertussis-cohorts during months -1 to +11 months was £370 (95% CI 128-650; 26.4% higher; p=0.002).

Using data from the merged CPRD Aurum and GOLD databases, we estimated the incidence rate of reported pertussis among individuals aged ≥50 years with a diagnosis of asthma to be 9.6 events per 100,000 PYFU in England during 2009-2018, with a peak of 20.9 in 2012. This is approximately twice as high as the estimated incidence rate among a general cohort of adults If only one event count in a stratification block was 1-4 and a total is given, the next lowest non-zero event count is marked "low" to prevent identification of any exact cell count of 1-4. b Most recent record from -18 months to before -6 months. c Most recent record during the 18 months before the index date. d Most recent BMI ≥30 kg/m 2 during the 18 months before the index date, or obesity in the clinical record during the 18 months before the index date (and no subsequent BMI <30 kg/m 2 before the index date). e At any time before the index date. f Number of hospitalizations for ≥1 night with a primary diagnosis code of asthma, excluding admissions <14 days after a previous admission, during the 18 months before the index date. g A prescription for a medium-or high-dose inhaled corticosteroid/long-acting beta 2 agonist combination during the 18 months before the index date. h Baseline DMC included costs for general practitioner/nurse, outpatient specialist, and accident and emergency visits, and inpatient stays from -18 months to before -6 months. aged ≥50 years in England in a study that used the same methodology across the same time period (5.8 events per 100,000 PYFU; 12.4 in 2012). 36 The cyclic nature of the yearly estimated incidence rates among older adults with a diagnosis of asthma in the current study were well aligned with the pertussis cases reported by Public Health England, 6 including the pertussis outbreak in England in 2012 (laboratory confirmed cases: 18 per 100,000 overall; 6 notifications: approximately 7 per 100,000 in those aged ≥45 years). 5, 37 We also observed that the incidence rate of diagnosed pertussis declined with increasing age, possibly related to reduced exposure or increased under-recognition of pertussis among older adults. 14 In the current study, patients in the asthma+/pertussis+ cohort had increased HCRU compared to those in the asthma+/pertussis-cohort, highlighting the clinical implications of pertussis infection among those with a diagnosis of asthma. Underlying this increase may be a combination of pertussis diagnosis and treatment of exacerbated asthma symptoms. In a Canadian study, asthma symptoms worsened in individuals with pertussis, as reflected by increased bronchodilator use in >90% of patients with preexisting pulmonary disease (mainly asthma). 3 Those with vs without asthma also had a longer mean duration of paroxysmal cough and more nights disturbed by pertussis. 3 In a US study, all-cause and pertussis-related hospitalization and health care expenses were higher in patients with diagnoses of asthma and pertussis than in those with pertussis alone. 21 The present data add to the consensus that pertussis has a substantial impact in people with asthma.

In the current study, GP/nurse visits, GP prescriptions, A&E visits, and hospitalizations started to increase in the asthma+/pertussis+ cohort from approximately 3 months before a pertussis diagnosis. This indicates that patients may seek medical care for symptoms well in advance of being diagnosed with pertussis, 16 which is in line with a typical cough duration of 3 months. 3 In the asthma+/per-tussis+ vs asthma+/pertussis-cohort during months -6 to -1, there were significantly more prescriptions for oral prednisolone -which is recommended for asthma exacerbations 38 -and amoxicillin -which is known to be prescribed for patients with asthma and lower respiratory tract infections (LRTIs), ie infective asthma exacerbations. 39 In the month before a pertussis diagnosis, there were significant excesses in the use of prednisolone and salbutamol -suggesting nonresolving asthma symptoms -and clarithromycin -which is one of the antibiotics recommended for people with suspected or confirmed pertussis, 40 but is also a second-line antibiotic for patients with asthma and LRTIs. 39 Omeprazole prescriptions were also increased, but only in the asthma+/pertussis+ cohort, possibly indicating symptom overlap between pertussis and gastroesophageal reflux disease. 41 Prescriptions for clarithromycin were also significantly elevated during the 2 months from diagnosis. Although it should be noted that we only analyzed the top five prescriptions (in the asthma+/pertussis+ cohort during each time period), this treatment pattern suggests that it often took time to arrive at a pertussis diagnosis, during which time patients may have been experiencing acute exacerbations of asthma and/or have been treated for an LRTI (whether misdiagnosed pertussis, another LRTI, or coinfection). A study in the US has also reported that some children with pertussis were originally misdiagnosed with an asthma exacerbation, 42 while a Chinese study reported B. pertussis in nasopharyngeal swabs from 28% of children with acute asthma exacerbations. 43 During the 5 months from a pertussis diagnosis, some HCRU continued to be elevated, suggesting medical follow-up. Overall, the study indicates an increased need for HCRU and a lengthy time to diagnosis, which is concerning given the recommendation to treat pertussis early in its course in order to help improve recovery and prevent transmission. 44 The potential adverse effects of systemic corticosteroids have been highlighted by others, 45 

elevated use of oral prednisolone in the asthma+/pertussis + cohort further reinforces the benefits of preventing pertussis in patients with asthma. However, further studies are required to ascertain whether pertussis prevention could reduce corticosteroid use in patients with asthma. Patients aged ≥50 years in the asthma+/pertussis+ cohort had an estimated mean excess annual DMC of £370 compared to those in the asthma+/pertussis-cohort. This total cost (including diagnosis) is generally in line with the mean cost of treating a case of acute respiratory illness during influenza seasons in 2001-2009 in a general population in another CPRD study in the UK, which reported costs of £116 for those aged 50-64 years and £478 for those aged ≥65 years. 46 Recently, national and international organizations reinforced their adult tetanus, diphtheria, and acellular pertussis (Tdap) and other vaccine recommendations [47] [48] [49] [50] in order to maintain good health in vulnerable age groups during the COVID-19 pandemic and to reduce the burden of pertussis on healthcare systems. In the US, the Centers for Disease Control and Prevention recommends that all adults receive one dose of Tdap vaccination if not previously received at or after 11 years of age (reflected in the 2021 Global Initiative for Chronic Obstructive Lung Disease guidelines 51 ) followed by 10-yearly Tdap or tetanus-diphtheria vaccination. 52 Adults are also recommended to receive various other vaccines, including those against influenza (all ages) and pneumococcal disease (age ≥65 years). 52 Younger adults with lung disease are also recommended to receive pneumococcal vaccination. 52 In the UK, adult pertussis vaccination is recommended for pregnant women, 53 and indeed, none Currently, the only vaccinations that are additionally recommended for adults with asthma in the UK are influenza and pneumococcal vaccinations, but only for those with severe asthma (defined as "asthma that requires continuous or repeated use of inhaled or systemic steroids or with previous exacerbations requiring hospital admission" for influenza vaccination and "so severe as to require continuous or frequently repeated use of systemic steroids" for pneumococcal vaccination). 54

Strengths of our study include the use of a large observational dataset that is generalizable to all adults with a diagnosis of asthma in England and contains demographic and clinical information data in primary and secondary care.

Using medical codes to define asthma is a limiting factor because we could not ascertain how the initial diagnosis was made nor potential concurrent diagnosis of asthma and pertussis. Using medical codes to define pertussis cases is also a limitation, as we do not know how they were diagnosed. However, our epidemiological patterns in pertussis incidence rates during 2009-2018 were well aligned with Public Health England data on laboratory confirmed pertussis cases during this period, 6 bringing credence to our method. Although our incidence rates should be a reasonable approximation of laboratory confirmed pertussis, it is highly likely that the true pertussis incidence is considerably higher, as individuals who did not seek health care for their symptoms and those whose pertussis was misdiagnosed or not reported would not have been captured.

Although quality control checks were conducted, we could not assess the validity and completeness of individual patient records. Also, deprivation measures (IMD) were included in the propensity score matching, but this was estimated based on residence postcode rather than individual measures.

More patients in the asthma+/pertussis+ vs asthma +/pertussis-cohort had chronic/persistent cough, with unknown underlying causes. There may also have been imbalances in unmeasured factors. However, other baseline characteristics and DMC were well balanced, and HCRU was similar apart from around the pertussis diagnosis (Figure 4 ), so any impact should be small. It is possible that some of the excess health care use in the asthma+/pertussis+ cohort was not related to pertussis, although having a matched comparator cohort should have reduced the risk of confounding. However, we could not capture the costs of treating pertussis among Figure 5 Annualized per-patient DMC a in the asthma+/pertussis+ and asthma+/pertussis-cohorts during the various time periods of the study. Notes: a Values above bars show total mean (95% CI) annualized DMC per patient. 95% CI were obtained assuming Student's t distribution for the annualized DMC means. Absolute mean values and standard deviations can be found in Supplementary Table 10 . p-values were calculated using the Wilcoxon rank-sum test. Uncorrected p<0.001 is displayed in bold and indicates statistical significance after cut-off adjustment for multiplicity. Unit costs for GP, nurse, and outpatient specialist visits were derived from the 2019 Unit Costs of Health and Social Care published by the Personal Social Services Research Unit from the University of Kent. 25 Unit costs for the five most commonly prescribed medications (in the asthma+/pertussis+ cohort; during each time interval) were derived from the September 2019 NHS Drug Tariff. 26 Unit costs for the five most common clinical assessments and A&E costs were derived from the 2018/2019 National Schedule of NHS Reference Costs. 27 

Dovepress patients with undiagnosed pertussis. Although the CPRD contains data on drug prescriptions, it does not contain information on why each drug was prescribed nor drug consumption. Also, only the top five prescription medications (in the asthma+/pertussis+ cohort; during each time period) were included in the DMC estimations, hence actual prescription costs would have been higher, although the impact on the overall DMC would have been small compared to the other costs that were included. Lastly, we did not include non-direct costs (such as workdays lost and over-the-counter medications) or quality of life measures, as these are not available in the data. Had we been able to include these factors, the burden of pertussis (in terms of both cost and morbidity) would likely have been higher.

The incidence rate of reported pertussis among adults aged ≥50 years with a diagnosis of asthma in England was 9.6 per 100,000 PYFU. Reported pertussis among adults with a diagnosis of asthma resulted in significant excesses of HCRU, which started several months before diagnosis and persisted for months after diagnosis, suggesting lengthy time to diagnosis and to optimal treatment, and prolonged impact of pertussis in this population. Subsequently, DMC were significantly elevated in the asthma+/pertussis+ cohort compared to the asthma+/pertussis-cohort. These results highlight the need for increased awareness of the potential for pertussis infection among older adults with a diagnosis of asthma. If such patients with clinical suspicion of pertussis were more routinely tested for this infection, this could result in more -and earlier -diagnosis and treatment, potentially reducing HCRU, DMC, and risk of transmission. Our results also highlight the need to improve preventive measures in this population. 

Ventolin and Evohaler are trademarks owned by or licensed to the GSK group of companies.

The datasets generated and/or analyzed during the current study are not publicly available as CPRD data are accessed via a license, the terms of which do not allow for the sharing of raw data.

The study protocol received CPRD ethics approval via the Independent Scientific Advisory Committee on March 4, 2020 (protocol number 20_043). The study complied with all applicable laws regarding subject privacy. No direct subject contact or primary collection of individual human subject data occurred, and no consent was necessary.

GP prescriptions

CHEST Expert Cough Panel. Clinically diagnosing pertussis-associated cough in adults and children: CHEST guideline and expert panel report

Morbidity of pertussis in adolescents and adults

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The authors are grateful to Esse Ifebi Herve Akpo and Elisa Turriani (GSK, Belgium) for their support during the early phases of the study. The authors would like to thank Business & Decision Life Sciences platform for editorial assistance, writing support and manuscript coordination, on behalf of GSK. Gauhar Masgutova and Aurélie Roth (Business & Decision Life Sciences, on behalf of GSK) coordinated manuscript development and editorial support. Jenny Lloyd (Compass Medical Communications Ltd., on behalf of GSK) provided medical writing support. Part of the results of this paper was presented at the European Respiratory Society (ERS) Conference 2020. The poster's abstract was published in a supplement of the European Respiratory Journal (https://doi.org/10.1183/13993003.con gress-2020.4926, vol 56 Issue suppl 64, 2020). Part of the results have also been presented at ECCMID 2021, but the abstract was not published in an accessible journal. This study is based in part on data from the Clinical Practice Research Datalink obtained under license from the UK Medicines and Healthcare products Regulatory Agency. However, the interpretation and conclusions contained in this report are those of the authors alone. 

Amit Bhavsar, Emmanuel Aris, Lauriane Harrington, Kinga Meszaros, Nicolas Jamet, Yan Sergerie and Piyali Mukherjee are employed by the GSK group of companies. Emmanuel Aris, Kinga Meszaros, Yan Sergerie, and Piyali Mukherjee hold shares in the GSK group of companies. Jason C Simeone and Anna Ramond were employed by Evidera Inc. during the time of the study, which received funding from the GSK group of companies to complete the work disclosed in this manuscript. Jason C Simeone's current affiliation is at Real-world Analytics, Cytel Inc, Waltham, MA, USA. Dimitra Lambrelli is employed by Evidera Inc., which received funding from the GSK group of companies to complete the work disclosed in this manuscript. Alberto Papi is a board member of the GSK group of companies, AstraZeneca, Chiesi Farmaceutici, Mundipharma, Novartis, Roche, Sanofi/Regeneron and Zambon. He also declares having received consulting and lecturing fees from the GSK group of companies, AstraZeneca, Avillion, Boehringer Ingelheim, Chiesi Farmaceutici, Mundipharma, Novartis, Sanofi/Regeneron, TEVA and Zambon; consulting fees from Edmond Pharma, Elpen Pharamceuticals, IQVIA, MSD and Roche; lecturing fees from Menarini; grant support from the GSK group of companies, AstraZeneca, Boehringer Ingelheim, Chiesi Farmaceutici, Fondazione Chiesi, Fondazione Maugeri, Menarini, and TEVA; grand support for vaccine studies from the GSK group of companies; and travel expenses reimbursement from the GSK group of companies, AstraZeneca, Boehringer Ingelheim, Chiesi Farmaceutici, Menarini, Mundipharma, Novartis, Roche, Sanofi/Regeneron, TEVA, and Zambon. Louis-Philippe Boulet reports, during the past 36 months, having received support for attending meetings from AstraZeneca and Novartis; research grants having been provided to his institution for participation in multicenter studies from the GSK group of companies, Amgen, AstraZeneca, Merck, Novartis, Sanofi-Regeneron. He also reports that his group of respirologists has received consulting fees from the GSK group of companies, AstraZeneca, Merck, Novartis, Sanofi-Regeneron; lecturing fees from the GSK group of companies, AstraZeneca, Covis, Merck and Novartis; and royalties. He is also Chair of the Board of Directors of the Global Initiative for Asthma (GINA), First Vice-president of the Global Asthma Organization «InterAsma», member of the Canadian Thoracic Society Respiratory Guidelines Committee, member of the Canadian AllerGen Network of Centres of Excellence, Chair of Knowledge Transfer, Prevention and Education in Respiratory and Cardiovascular Health in Laval University and member of scientific committees for the American College of Chest Physicians, American Thoracic Society, European Respiratory Society, World Allergy Organization, GARD (World Alliance against Chronic Respiratory Diseases, WHO), FIRS and ARIA. These authors declare no other financial or non-financial relationships and activities.

All named authors meet the International Committee of Medical Journal Editors (ICMJE) criteria for authorship for this article. All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

GlaxoSmithKline Biologicals SA funded this study (study number HO-18-19690) and was involved in all stages of study conduct, including analysis of the data. GlaxoSmithKline Biologicals SA also took in charge all costs associated with the development and publication of this manuscript.