key: cord-0017229-hfvve1w7
authors: Aftabsavad, Somayeh; Noormohammadi, Zahra; Moini, Ashraf; Karimipoor, Morteza
title: Effect of bisphenol A on alterations of ICAM-1 and HLA-G genes expression and DNA methylation profiles in cumulus cells of infertile women with poor response to ovarian stimulation
date: 2021-05-05
journal: Sci Rep
DOI: 10.1038/s41598-021-87175-1
sha: ab8a6caf1f548186f3b14e620f93c40b005178a6
doc_id: 17229
cord_uid: hfvve1w7

This study aimed to investigate the relationship between follicular fluid Bisphenol A (BPA) concentrations with alterations of ICAM-1 and HLA-G genes and proteins expression as well as methylation profiles in the cumulus cells of poor ovarian response (POR) women based on their healthy lifestyle habit. Eighty women under the age of 35 were divided into two groups: 1—POR without using plastic containers (n = 40) and 2—POR with using plastic containers (n = 40). The ICAM-1 and HLA-G genes and protein expressions were examined by the quantitative PCR and western blotting technique. The methylation pattern was investigated by the methylation-specific PCR. Total BPA in follicular fluid was measured with high-performance liquid chromatography technique and the detection limit was 1.14 ng/ml. ICAM-1 and HLA-G genes were differentially expressed between the two groups studied. ICAM-1, HLA-G genes, and protein expressions in group 1 were up-regulated compared to the second group (P < 0.05). While DNA methylation status in group 1 were decreased compared to the other group (P < 0.05). The concentration of BPA in the follicular fluid of group 1 was lower compared to the second group (P < 0.05). The oocyte quality and clinical pregnancy ratio showed significantly higher in group 1 than in the other ones (P < 0.05). The alteration of ICAM-1 and HLA-G gene expressions in POR women is probably related to BPA concentration. As a result Lifestyle habits may also affect the methylation pattern and protein levels in the cumulus cells of POR women. Additionally, lifestyle habits may be considered as a marker for ovulation, oocyte maturation, preimplantation, and clinical pregnancy process.

The term poor response to ovarian stimulation (POR) typically refers to woman with a reduced ovarian reserve or poor ovarian response to exogenous gonadotropin stimulation 1 . In fact, the POR or the cycle without ovulation is a cycle in which the ovary is unable to release the oocyte. As a result, ovulation does not occur. Despite the efforts to optimize the definition of POR, unfortunately there is still limited knowledge about the POR pathophysiology, and the etiology hinders the practical solutions to manage the condition 2 . Indeed, the diagnosis of anovulation is not easy. Contrary to popular belief, women with anovulation have more or less a normal menstrual cycle and usually for the first time, they find their own problem when they want to become pregnant 3 . Recently, POSEIDON criteria have been used to diagnose POR, including age, Anti Müllerian Hormone (AMH) dose, Antra Follicle Count (AFC), and oocyte number 4 . The consequential complications of POR are intrinsic ovarian resistance to gonadotropin stimulation, reduced ovarian reserve, ovulation and oocyte maturation disorder 5 . Reduced ovarian reserve is defined as the impaired fertility ability leading to infertility. In fact, excessive use of plastics objects in the human life has been proposed as one of the causes of creating POR in women 6 . Indeed, excessive use of plastics to store hot food and drinks can cause various diseases. Polycarbonate is a type of plastic containing BisphenolA (BPA) 7 . Over time, when plastic is heated or exposed to acidic or alkaline substances, it breaks down and BPA attaches to food and drink, so that it can enter the body 8 . BPA, by mimicking sex hormones, can disrupt the endocrine system, hormones, hormonal signaling pathways and gene expression 9 . BPA even in small amounts may interfere with the ovulation, ovarian response, oocyte maturation, pregnancy and genes function 10 . In fact, lifestyle habit plays a key role in regulating ovarian response, oocyte maturation and genes expression. Gene's expression alteration in POR patients is a condition damaging oocyte developmental, resulting in reduced rates of fertilization, embryonic development, and implantation 11 . The role of gene's expression alteration in the pathophysiology and etiology of POR is uncertain, but studies support the hypothesis that nutrition and lifestyle habit play a crucial role in regulating the response of intrinsic ovarian resistance to gonadotropin stimulation, reduced ovarian reserve, oocyte maturation, implantation and gene expression 12 .

ICAM-1, known as CD54, plays a pivotal role during oocyte maturation, and it is a protein encoded by the ICAM1 gene. The responsibility of ICAM-1 is encoding a cell surface glycoprotein usually expressed on endothelial cells and cells of the immune system. As mentioned, ICAM-1 plays a central role in the ovulation process 13 . Indeed, the main components of this ovulation process are the disruption of the extracellular matrix (ECM) at the follicular peak and the change in follicular vessels. In these events, ICAM-1 participates by secreting proteases and active inhibitors. Indeed, biochemical markers of the oocyte maturation and ovulation are highly important. Upregulation of ICAM-1 in immature oocytes as well as downregulation of ICAM-1 in mature oocytes has been observed 14 . Human leukocyte antigen-G (HLA-G) is regarded to play a vital role in oocyte maturation and implantation of embryos. HLA-G can be effective in controlling trophoblast invasion and maintaining a local immunosuppressive state. The expression and distribution of HLA-G is on human spermatogenic cells, primary and secondary oocytes, and preimplantation embryos. Furthermore, the HLA-G production is related to the good quality of cumulus cells (CCs) in oocytes 11 .

In the present study, we aimed to assess the relationship between follicular fluid BPA concentrations with genes expression, proteins level and methylation status of ICAM-1 and HLA-G in the cumulus cells of infertile POR patients based on their lifestyle habit following ovarian stimulation with a gonadotropin releasing hormone (GnRH) antagonist protocol.

Patient selection. In the present study, eighty women under the age of 35 and BMI 18-25 kg/m 2 , who participated in an intracytoplasmic sperm injection (ICSI) program, were selected. On the day of ovarian puncture, follicular fluid sampleof each woman was collected in a special tube (without using BPA compound, it is called healthy lifestyle habit here) and then stored at − 70 °C until analysis. The patients who were diagnosed by the Poseidon group1 subgroup 1b that included; [patients < 35 years old with adequate ovarian reserve parameters (AFC > 5; AMH > 1.2 ng/ml) and with an unexpected poor or suboptimal ovarian response, and 4-9 oocytes retrieved] (4). The patients were divided into two groups: without a healthy lifestyle habit (control group contain, n = 40, infertile women with poor response (POR) to ovarian stimulation history), and with a healthy lifestyle habit (case group contain, n = 40, infertile women with poor response (POR) to ovarian stimulation history). The healthy lifestyle habit group included women who used little plastic containers for hot food, hot drink and foodstuffs store. However, the group without a healthy lifestyle habit included women who used excessive plastic containers to store hot food, hot drink and foodstuffs. Information on a healthy lifestyle habit was obtained using a validated food frequency questionnaire. Our questionnaire includes precise questions such as; how much they use plastic containers for the hot food, hot drink, and foodstuffs store in their daily life. The main exclusion criteria were women affected by endometriosis, and women with a history of uterus and ovaries operation. Fur- www.nature.com/scientificreports/ thermore, in this study, at the request of the patients, the treatment IVF/ICSIcycle was canceled, while they had a low number of follicles or lacked growth of follicles. The Ethics Committee at Royan Institute, Iran approved this prospective case-control study (No. IR.ACECR.ROYAN.REC.1394.150). All participants gave informed consent prior to inclusion in the study. We ensured the confidentiality of the patients' identities by data anonymization during analysis. This research did not affect the treatment of patients.

Flexible antagonist stimulation protocol. In this study, controlled of ovarian stimulation (COS) was controlled from the third day of the cycle. The patients received regular, daily subcutaneous (SC) injections of recombinant follicle stimulating hormone (rFSH, Gonal-F, Serono, Switzerland). The first dose of rFSH for each patient was started according to sonographic monitoring, and AFC, estradiol (E2) level, and AMH for each patient were evaluated. In the stage of growing follicles > 12 mm, the patients received SC injections of a GnRH antagonist, cetrorelix (Cetrotide, Merck Serono, Germany). The protocol consisted of daily Cetrotide SC injections until the criteria for human chorionic gonadotropin (hCG) administration were met. When more than 3 follicles reached diameters of at least 18 mm and E2 levels of 1000-4000 pg/mL When at least three follicles reached diameters of ≥ 18 mm as well as E2 levels of 1000-4000 pg/mL, each patient received an intramuscular (IM) injection of 10,000 IU of hCG (Pregnyl, Organon, Netherlands) or SC injection of 250 μg Ovidrel (Merck Serono, Germany).

Isolation of cumulus cells. Following follicular puncture, the cumulus-oocyte complexes (COCs) were collected and washed at least 3-5 times in G-IVFTM medium (Vitrolife, Sweden) to remove blood and excess cells. Immediately after washing, the COCs were moved to a CO 2 incubator at 37 °C for 2 h in G-IVFTM medium. Cumulus cells were denuded from COCs by 80 IU of hyaluronidase, (Sigma, USA). Cumulus cells from each mature oocyte (100 in POR with healthy life style versus 100 in POR without healthy life style) were collected in individually tubes. After oocyte denudation, a pellet of cumulus cells was washed with phosphatebuffered saline (PBS), and RNA stabilizer reagent buffer was added for further analyses. Cumulus cells were immediately transferred to liquid nitrogen for snap freeze, and then they were stored at -80 °C. The cumulus cells were denuded from metaphase II gametes (MII). In the IVF laboratory, MII gametes were fertilized by the ICSI process within 10 min after denudation, and they were incubated until the embryo transfer was processed. Fertilization was evaluated (16-20 h after ICSI). According to our IVF laboratory standards process, embryos were graded by embryologist specialists at the pronuclear (16-20 h) and cleavage stages (48-72 h). Then, the embryologist selected 1 or 2 embryos for transfer. For successful embryo transfer, they considered the embryo grades, age of the patients, and previous ART cycles. The quality of the embryos at the cleavage stage was classified based on these criteria: [The excellent quality of embryo should be (≥ 4 cells or ≥ 8 cellsand < 10% fragmentation), the good quality of embryo should be (≥ 4 cells or ≥ 8 cells and 10-20% fragmentation), and the poor quality of embryo should be (< 4 cells or < 8 cells and > 20% fragmentation)] 15 .

The samples were analyzed at Analytical Chemistry Laboratory of Sharif University. Total BPA (conjugated and free) in follicular fluid was measured using high-performance liquid chromatography (HPLC). Briefly, at the first, in the glass tubes reaction mixtures contains of phosphorous acid buffer, β-glucuronidase (Sigma, USA), and sample aliquots was made. Afterwards, hydrolyzations at 37 °C were done, and then extracted twice with ether (HPLC grade, Merck, Germany). With the help of nitrogen gas flow, the supernatants were collected and evaporated. The remaining material was dissolved in 60% acetonitrile (HPLC grade, Merck, Germany) and HPLC was used for analysis: A KNAUER liquid chromatograph (KNAUER, Germany) with a RF-20A bulk fluorescence detector with an excitation wavelength of 275 nm and an emission wavelength of 300 nm was used. Chrom Gate software version 3.3 (KNAUER, Germany) was used for data processing. In the following, we used 5 μM Columns (Chromolith Performance RP-18e, USA), and columns 100 × 4.6 mm for liquid chromatography, and 20 μl injection loop, mobile phase A and B, acetonitrile/ water (40:60, v/v), equivalent grade; and flow: 1.0 mL/min. HPLC water was used from Millipore Super-Q Plus Water Purification System (Bedford, MA, USA). In this experiment, for the calculating of limit of detection (LOD) we used of the Environmental Protection Agency method (2004). The LODs of BPA in follicular fluid were 1.14 ng/ml. qPCR. Total RNA was extracted by a Trizol (TRI; Sigma-Aldrich, USA) and treated with RNase-free DNa-seI according to the manufacturer's instructions. RNA concentration and purity were quantified using a Nan-odrop2000 Spectrophotometer (Thermo, USA). Reverse transcription was performed at 25 ºC for 10 min, 42 ºC for 1 h and then 70 ºC for 10 min. For this reaction, cDNA was synthesized using the Revert AidTM H-Minus First Strand cDNA synthesis (Cat NO: K1631, Fermentas, Germany) by the random Hexa nucleotides primer (0.2 µg/µl) in a solution (20 µl) containing 4 µl 5× reaction buffer, 1 µl Ribonuclease inhibitor (20 U/µl), 2 µl dNTP mix (10 mM) and RNase-DNase free water. ICAM-1 and HLA-G were chosen as target genes, and 18srRNA was utilized normalizae each sample. Primers were designed by the Primer Express 3.0 software for the real-time PCR. Table 1 presents the primer sequences. The real-time PCR was performed in a StepOnePlus instrument (Applied Biosystems, USA). The reaction contained 2 µl cDNA, 10 µl of Power SYBR Green Master mix (Cat NO: RR420A, TAKARA, Japan) and 1 µl (of 500 nM primer) of forward and reverse primers. The reactions were performed in duplicate. The thermal cycling condition for the amplification was 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s and 60° for 10 min. The Ct data were determined using default threshold settings. The 2 −ΔΔCT method was used to analyze the relative expression level of target genes. Melt curve analysis was performed to determine the specificity of the real-time 1 ).

Chemiluminescence detection system (UVITEC, UK) was used for protein visualization. The ImageJ software version 1.50i (US National Institutes of Health, Bethesda) was used to quantify protein bands intensity on the PVDF paper 16, 17 . The changes in ICAM-1 and HLA-G level were normalized against β-actin as a housekeeping protein and then calculated regarding infertile women with a poor response to ovarian stimulation without a healthy lifestyle habit.

Methylation-specific polymerase chain reaction (MSP). Genomic -GCA ATG TGC AAG AAG ATA GCCA-3′  105  R-ICAM-1  5′-GGG CAA GAC CTC AGG TCA TGT-3′   F-HLA-G  5′-AGC TGT GGT GGT GCC TTC -3′  106  R-HLA-G  5′-GGG CAG GGA AGA CTG CTT -3′   F-18srRNA  5′-GTA ACC CGT TGA ACC CCA TT-3′  151  R-18srRNA  5′-CCA TCC AAT CGG TAG TAG CG- (Table 1) . For MSP positive control, we used fully methylated DNA (100%) and unmethylated DNA (0%), which the fully methylated DNA was made of MsssI methylase (NEB, Ipswich, MA, USA) using human placental genomic DNA (gDNA; Sigma Aldrich, USA), and the unmethylated DNA was made of human placental genomic DNA (gDNA; Sigma Aldrich, USA). For MSP method sensitivity, we used the fully methylated and unmethylated DNA (EpiTect Control DNA and Control DNA Set, Qiagen, Germany) with different serial dilutions: the ratio 0% to 100%, 5% to 95%, 10% to 90%, 20% to 80%, 35% to 65%, 50% to 50%, 75% to 25% and 100% to 0% unmethylated DNA was combined with methylated DNA, respectively. Thermo cycling conditions were as follows: 95 °C for 5 min followed by 40 cycles of 95 °C for 30 s, 63 °C for 60 s and 72 °C for 60 s, with a final extension of 72 °C for 4 min. MSP products for methylated and unmethylated ICAM-1 and HLA-G promoters were run on 2% agarose gels containing 40 mMTris-acetate/1.0 mM EDTA (pH = 8) and were visualized by ethidium bromide staining (Fig. 2) . Methylation genes pattern visualization was performed using the enhanced chemiluminescence detection system (UVITEC, UK). The intensity of each MS-PCR bands on the agarose gel was quantified using the ImageJ software version 1.50i (http:// imagej. nih. gov/ ij/; provided in the public domain by the National Institutes of Health, Bethesda, MD, USA).

Relative gene expressions were calculated by the 2 −ΔΔCt method. The normalized ΔCt value of each sample was calculated using reference genes. In this study, categorical variables were presented as number (%) and continuous variables as mean ± SD. The independent t-test was used to assess the mean differences in demographic and clinical characteristics between the groups. Chi-square analysis was used for qualitative data. Univariate and backward multiple linear regression, including all variables, were used to evaluate the association between BPA concentration and ICAM-1 and HLA-G genes, proteins, methylation and demographic and clinical variables. Statistical analyses were conducted using the IBM SPSS Statistics for Windows, Version 22.0 (IBM Crop., Armonk, NY, USA). All statistical tests were 2-tailed, and a P < 0.05 was considered statistically significant.

Informed consent. All participants gave informed consent prior to inclusion in the study. All methods were carried out in accordance with relevant guidelines and regulations. 

Demographic data analysis. Table 2 presents the participants' demographic and clinical characteristics.

The number of oocytes was significantly higher in the healthy lifestyle habit group (Group1) than in the group without a healthy lifestyle habit (Group 2), (3.83 ± 0.99 vs. 3.27 ± 0.64, p = 0.004, respectively). The number of the MII retrieved oocyte was higher in Group 1 than in Group 2 significantly (2.83 ± 0.99 vs. 2.35 ± 0.66, p = 0.013, respectively). The clinical pregnancy rate (fetal heart detection by ultrasound) was significantly different between the two groups. Indeed, the clinical pregnancy rate was higher in the healthy lifestyle habit group than in Group 2 (1.80 ± 0.41 vs. 1.70 ± 0.46, 0.040). In the clinical variables of total embryo number and oocyte quality between two groups was significantly difference. Actually, the number of total embryo and oocyte quality was significantly higher in Group 1 than in Group 2 (oocyte quality: 3.51 ± 1.05 vs. 2.50 ± 0.93, p = 0.000; total embryo: 1.93 ± 026 vs. 1.30 ± 0.56, p = 0.000). In addition, a significant difference was observed in the AFC level between the two groups (p = 0.05). On the other hand, in the age, marriage duration, infertility duration, BMI, and AMH level of patients, there was no difference significantly. After adjusting all variables (such as; the clinical outcome, gene expression data, and protein expression data), based on the multiple linear regression model results, the AMH level had a negatively significant association with the ICAM1 and HLA-G proteins expression (p = 0.018); for each unit increase in the AMH level, the expected oocyte quality was decreased by 0.14. The multiple linear regression model indicated that the number of MII was positively associated with the ICAM1 and HLA-G proteins expression (p = 0.021); for each unit increase in AMH, the expected ICAM1 and HLA-G proteins expression was increased by 0.04. This multivariate model revealed a negatively association with ICAM1 and HLA-G genes in two groups studied. Although the ICAM1 and HLA-G proteins expression in women without a healthy lifestyle habit were decreased by nearly 13 times than in women with a healthy lifestyle habit (p = 0.000), the expected ICAM1 and HLA-G proteins expression were decreased by nearly 0.23 times in women without a healthy lifestyle habit than in women with a healthy lifestyle habit (p = 0.000, Table 4 ). The rest of the variables included in the univariate model (Table 3) were not significantly associated with oocyte quality in multiple models. ICAM1 and HLA-G mRNA, protein expressions, and DNA methylation pattern. The mean relative expression of ICAM1 and HLA-G genes was significantly increased in Group 1 (p = 0.000) than in Group 2 (Fig. 3a) .

For analysis of HLA-G and ICAM proteins, western blotting test was performed. The data showed ICAM1 and HLA-G proteins had expression in the cumulus cells of POR patients (Fig. 1) . Analysis of protein bands intensity data was significantly increased in Group 1 (p = 0.000) than Group 2 (Fig. 3b) .

The methylation pattern of HLA-G and ICAM-1 genes was evaluated by MS-PCR (MSP, Fig. 2 ). Analysis of MSP bands showed that a notable increase in ICAM1 and HLA-G DNA methylation in Group 2 compared to Group 1 (p = 0.000, Fig. 3c) .

The mean of DNA methylation index for HLA-G and ICAM-1 genes in the cumulus cells were significantly lower in Group 1 compared with Group 2, (0.13 ± 0.008, 0.15 ± 0.007, 0.25 ± 0.008 and 0.27 ± 0.009, p = 0.000, respectively, Fig. 3c) . www.nature.com/scientificreports/ BPA concentration and the association with expressions and DNA methylation pattern. The mean concentration of BPA in follicular fluid was statistically significantly lower in Group 1 than in Group 2 (1.56 ± 1.33 ng/mL and 4.73 ± 2.23 ng/mL, P < 0.0001 respectively), (Table 2) . Based on the univariate analysis (Tables 3, 4 ), oocyte number (p = 0.03), oocyte quality, total embryo, and type of group (p = 0.000) in the groups studied (women with and without a healthy lifestylehabit) were significantly associated with BPA concentration, ICAM1 and HLA-G gene expression and DNA methylation.

Variables of oocyte number, oocyte quality, total embryo, MII number and type of group were significantly associated ( Table 2 ) with ICAM1 and HLA-G proteins expression in the groups studied.

In the present study, our finding showed that concentration of BPA in follicular fluid of POR with healthy lifestyle was lower compared to the POR without healthy lifestyle habit, also, ICAM-1and HLA-G transcripts and proteins in POR with healthy lifestyle habit were upregulated compared to the POR without a healthy lifestyle habit. In addition, the ICAM-1 and HLA-G DNA methylation status in the POR with a healthy lifestyle habit was hypomethylated compared to the POR without a healthy lifestyle habit. In fact, BPA can affect the transcript and proteins profile alterations in cumulus cells from POR patients. Furthermore, it may affect the methylation status in the cumulus cells of POR patients. Moreover, based on our data, the groups without a healthy lifestyle habit showed significantly lower numbers of good quality oocytes and clinical pregnancy rate compared to the group with a healthy lifestyle habit. As a result, BPA can affect the ovulation, oocyte maturation and clinical pregnancy rate in POR patients. Oocyte quality is one of the major limiting factors for the success of the ART cycle in POR patients 18 . Oocyte maturation is achieved during folliculogenesis. Folliculogenesis is the maturation of the ovarian follicle. Folliculogenesis needs communication between the oocyte and surrounding somatic cells 19 . One of the kinds of somatic cells is cumulus cells. Cumulus cells play a potential role in achieving oocyte development 20 . Based on a recent study, some genes are expressed in the cumulus cells that can be considered genetic markers for the association between oocyte and embryo quality. Probably, developmental signals from cumulus cells are transferred to the oocyte via gap junctions and pathways, which can affect the oocyte maturation 21 . The present study aimed to determine the novel essential marker to evaluate the oocyte quality and clinical pregnancy rate in the ART treatment of POR patients, and our data showed that lifestyle habit might be considered an essential marker for oocyte maturation increasing the clinical pregnancy rate. One of the consequent complications of POR is oocyte maturation disorder 5 . Nowadays, overuse of plastic objects to store hot food and hot drinks has caused numerous health problems in humans like reproduction disorders, including POR 22 . In fact, plastic objects made of polycarbonate and polycarbonates contain BPA 7 . According to the recent study, BPA may affect the infertility related gene expression that is closely associated with reproductive, but this association interaction mechanism is unclear. Owing to heat, acidic or alkaline substances, polycarbonate will break and BPA can attach to food and drink, and it can enter the body 6 . BPA can act like sex hormones, so that it can disrupt the endocrine system and hormonal signaling pathways. BPA in small amounts may interfere in the reproduction related gene expression, and it can interfere in the function of ovary, uterus and other reproductive organs 22 . Pednekar et al. 11 reported that infertile women showed significantly higher plasma concentrations of BPA. As a result, lifestyle habit plays a vital role in the regulating of ovarian response, oocyte maturation, reproductive process and genes expression. In this study, we evaluated the expression profile for ICAM-1 and HLA-G genes in the cumulus cells of infertile women with poor response to ovarian stimulation (POR) based on their healthy lifestyle habit. The probable effect of plastic objects usage was observed on the genes, proteins, DNA methylation pattern, oocyte quality and clinical pregnancy rate in these women. According to other studies, altered gene expression of CCs may play a role in women with POR pathogenesis 23, 24 . Therefore, assessment of genes expression is valuable to understand the etiology of infertile women with POR patients, since oocyte maturation dysfunction is one of the disorders in POR patients 11 . Recently, it has been shown that alternation of ICAM1 and HLA-G genes can affect the quality and quantity of oocytes 25 . Our findings revealed that a major contributor to oocyte maturity www.nature.com/scientificreports/ disorder and infertility in the POR patients without a healthy lifestyle habit can be a reduction in ICAM1 and HLA-G transcript and proteins expression as well as hypermethylation of DNA in ICAM1 and HLA-G. According to recent studies, comparison of POR patients to healthy, normal ovulatory fertile women with a history of male infertility history has shown differential expression of ICAM-1 genes 26 .

The ICAM-1 gene expression pattern indicated that it is important in oocyte maturity and grading. It appeared that dysregulation of ICAM-1 expression could be associated with defective oocyte maturation and grading 27 .The studies demonstrated that ICAM-1 was a probable key effector of the oocyte maturation. Although the sICAM-1 release was very high in immature oocytes, it was decreased in mature oocytes. They regarded sICAM-1 as a biochemical marker for oocyte maturation and grading 11 . The ICAM-1 appears to play a critical role in oocyte development, while ICAM-1 levels play the role of a predictive marker in oocyte maturation and quality. Borgtti and Rizzo 25 proposed sICAM-1 as a biochemical marker for oocyte maturation and grading. Their findings supported a significant correlation between ICAM-1 gene and protein expression and oocyte maturation. A recent study has shown that ICAM-1 is expressed on leukocytes, epithelial and endothelial cells 11 . Furthermore, HLA-G plays a vital role in the oocyte maturation and preimplantation of embryos. Jurisicova et al. detected HLA-G mRNA expression by preimplantation human embryos 28 . They demonstrated the presence of HLA-G mRNA and protein in the blastocysts tested 13 . Yao et al. 29 revealed the expression of the HLA-G protein by human preimplantation embryos with an increasing ratio of positive embryos with developmental stage. Kaneko 28 indicated that the low expression of HLA-G in cumulus cells was associated with lower oocyte quality, poor fertilization, and reduced embryo development 29 . Rooij van reported the low level of HLA-G in the cumulus cells of POR patients 12 . The gene expression alternation in the cumulus cells of women with POR after dehydroepiandrosterone (DHEA) supplementation can affect the oocyte maturation 23 . According to recent study, oocyte quality decreases with increasing age 24 . Our findings indicated that age was directly associated with oocyte quality and aging. Furthermore, another study showed that younger POR patients' quality of oocyte and grading were better than older patients' 30 . Our study also highlighted the role of age as one of the components in oocyte maturation. The present study emphasized the association of lifestyle habit with the ICAM1 and HLA-G genes expression alteration and oocyte quality as an opinion for the deeper understanding of the etiology of infertile women with POR patients.

The current study was the first single center lifestyle program of POR patients in Iran. We found that alterations of ICAM1 and HLA-G in POR appeared to be related to BPA concentration. As a result nutrition and lifestyle habit are affecting in genes function. Indeed, the nutrition and lifestyle habit may be effective on the methylation pattern, expression profile and proteins level in cumulus cells from POR. Moreover, nutrition and lifestyle habit may act as an essential marker for ovulation and the oocyte maturation process.

Sample collection was the main limitation in our experiment as well as severe limitation of using laboratory facilities because of the COVID-19 pandemic situation.

They are available on the request. 

ESHRE working group on Poor Ovarian Response. Definition ESHRE consensus on the definition of 'poor response' to ovarian stimulation forin vitro fertilization: The Bologna criteria

Androgens (dehydroepiandrosterone or testosterone) for women undergoing assisted reproduction. Cochrane Database Syst

View authors' declarations of interest Interventions for 'poor responders' to controlled ovarian hyper stimulation (COH) in in-vitro fertilisation (IVF)

The novel POSEIDON stratification of 'Low prognosis patients in Assisted Reproductive Technology' and its proposed marker of successful outcome. F1000 Research

How to define, diagnose and treat poor responders? Responses from a worldwide survey of IVF clinics

Bisphenol-A and female infertility: A possible role of gene-environment interactions

Study on environmental and psychological risk factors for female infertility

Placental transfer of conjugated bisphenol A and subsequent reactivation in the rat fetus

Tetra bromo diphenyl ether (BDE 47) evokes estrogenicity and calbindin-D9k expression through an estrogen receptor-mediated pathway in the uterus of immature rats

The biomarker and endocrine disruptors in mammals

Release of sICAM-1 in oocytes and in vitro fertilized human embryos

Rapid evaluation of soluble HLA-G levels in supernatants of in vitrofertilized embryos

HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy

Embryonic soluble HLA-G as a marker of developmental potential in embryos

(1234567890) Scientific Reports | (2021) 11:9595 |

The transcriptome of corona radiata cells from individual MII oocytes that after ICSI developed to embryos selected for transfer: PCOS women compared to healthy women

Impact of insulin resistance on the developmental potential of immature oocytes retrieved from human chorionic gonadotropin primed women with polycystic ovary syndrome undergoing in vitro maturation

The alteration of PLCζ protein expression in unexplained infertile and asthenoteratozoospermic patients: A potential effect on sperm fertilization ability

Changes in the epigenetic status of the SOX-9 promoterin human osteoarthritic cartilage

Local roles of TGF-beta superfamily members in the control of ovarian follicle development

Granulosa cell-oocyte interactions

Apoptotic biomarkers in cumulus cells in relation to embryo quality in polycystic ovary syndrome

Evidence for bisphenol A-induced female infertility

Gene expression of cumulus cells in women with poor ovarian response after dehydroepiandrosterone supplementation

Women older than 40 years of age and those with elevated follicle-stimulating hormone levels differ in poor response rate and embryo quality in in vitro fertilization

Expression of intercellular adhesion molecules ICAM-1 and ICAM-2 in human endometrium: Regulation by interferon-g

Embryonics oluble HLA-G as a marker of developmental potential in embryos

Effect of hyaluronan on developmental competence and quality of oocytes and obtained blastocysts from in vitro maturation of bovine oocytes

Embryonic human leukocyte antigen-G expression: Possible implications for human preimplantation development

Differential expression of alternatively spliced transcripts of HLA-G in human preimplantation embryos and inner cell masses

Epigenetic age acceleration predicts cancer, cardiovascular, and all-cause mortality in a German case cohort

This study was funded by Royan Institute (code number: 94000163). The authors wish to thank Forough Azam Sayah pour and Maryam Jahangiri. The authors also would like to thank the patients for their cooperation. The author(s) declared no potential conflicts of interest with respect to the research, authorship, and publication of this article.

The authors declare no competing interests.

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