key: cord-0014751-c6pagqrb authors: Sullivan, Katherine P.; White, Heather O.; Grover, Lindsay E.; Negron, Jordi J.; Lee, Austin F.; Rhein, Lawrence M. title: Transcutaneous carbon dioxide pattern and trend over time in preterm infants date: 2021-01-19 journal: Pediatr Res DOI: 10.1038/s41390-020-01308-2 sha: 7d0c3a7844a2d33c7df053fc9e05008b8808c2a8 doc_id: 14751 cord_uid: c6pagqrb BACKGROUND: Chronic lung disease remains a burden for extremely preterm infants. The changes in ventilation over time and optimal ventilatory management remains unknown. Newer, non-invasive technologies provide insight into these patterns. METHODS: This single-center prospective cohort study enrolled infants ≤32 0/7 weeks. We obtained epochs of transcutaneous carbon dioxide (TcCO(2)) measurements twice each week to describe the pattern of hypercarbia throughout their hospitalization. RESULTS: Patterns of hypercarbia varied based on birth gestational age and post-menstrual age (PMA) (p = 0.03), regardless of respiratory support. Infants receiving the most respiratory support had values 16–21 mmHg higher than those on room air (p < 0.001). Infants born at the youngest gestational ages had the greatest total change but the rate of change was slower (p = 0.049) compared to infants born at later gestational ages. All infants had TcCO(2) values stabilize by 31–33 weeks PMA, when values were not significantly different compared to discharge. No rebound was observed when infants weaned off invasive support. CONCLUSIONS: Hypercarbia improves as infants approached 31–33 weeks PMA. Hypercarbia was the highest in the most immature infants and improved with age and growth despite weaning respiratory support. IMPACT: This study describes the evolution of hypercarbia as very preterm infants grow and develop. The pattern of ventilation is significantly different depending on the gestational age at birth and post-menstrual age. Average transcutaneous carbon dioxide (TCO(2)) decreased over time as infants became more mature despite weaning respiratory support. This improvement was most significant in infants born at the lowest gestational ages. This study describes the evolution of hypercarbia as very preterm infants grow and develop. • The pattern of ventilation is significantly different depending on the gestational age at birth and post-menstrual age. • Average transcutaneous carbon dioxide (TCO 2 ) decreased over time as infants became more mature despite weaning respiratory support. This improvement was most significant in infants born at the lowest gestational ages. Despite transformative improvements in the practice of neonatology over the past 20 years, the burden of chronic lung disease remains high among very low birth weight infants and those born very premature. Infants born at <32 0/7 weeks gestational age (GA) make up a small proportion of neonatal intensive care unit (NICU) admissions but represent a disproportionately large burden of long-term disease, particularly lung disease. [1] [2] [3] [4] In all, 25-40% of very low birth weight infants continue to be diagnosed with bronchopulmonary dysplasia (BPD), while in extremely low birth weight infants the rate of BPD is as high as 50-60%. [5] [6] [7] [8] [9] BPD is associated with increasing rates of poor neurodevelopmental outcomes, increased post-discharge readmissions, and increasedx mortality. 8, [10] [11] [12] [13] [14] [15] [16] [17] In an attempt to decrease BPD, various lungprotective strategies have been attempted. Gentle ventilation, using controlled tidal volumes and toleration of mild hypercarbia, has become increasingly common as a standard of care to avoid ventilator-induced lung injury. [18] [19] [20] [21] Studies have also demonstrated safety and a trend toward improved outcomes with tolerance of mild hypercarbia. [22] [23] [24] [25] However, more severe hypercarbia and large fluctuations of partial pressure of carbon dioxide (pCO 2 ) have been associated with multiple adverse events, including intraventricular hemorrhage (IVH) and mortality. [26] [27] [28] [29] Studies examining the long-term effects of a strategy of permissive hypercapnia have had mixed results. 26, [30] [31] [32] [33] It is also likely that the mechanism for alterations in ventilation may change over time during a premature infant's NICU course as lungs grow and changes associated with chronic lung disease develop. Studies examining respiratory management, particularly ventilation, have relied on measurement of blood gases to determine pCO 2 levels. Although this has been the standard of care, it has multiple disadvantages, including requiring invasive sampling techniques, cumulative blood loss for the infant, and reflection of a single point of time. Noninvasive methods of assessing pCO 2 , including capnography or transcutaneous monitoring, allow for collection of more frequent data points, including continuous monitoring without disturbing the infant. However, historically these methods have had several limitations in the neonatal population. Capnography using end tidal monitors may be inaccurate in infants for multiple reasons, including the use of uncuffed endotracheal tubes, the addition of a significant amount of dead space in the endotracheal tube-ventilator circuit, and the trend toward non-invasive ventilation. Use of transcutaneous carbon dioxide (TcCO 2 ) monitors has also been limited by technological pitfalls. Earlier technology had been associated with inconsistent accuracy, skin irritation, and burns. 34 Newer technology has made it possible to accurately and safely monitor TcCO 2 , 35-37 using heated skin probes that measure local CO 2 within the capillary bed and then adjusted to reflect arterial values. These more technologically sophisticated probes have been studied in multiple neonatal populations and show good correlation with arterial blood pCO 2 . 38, 39 Currently, there is a lack of understanding regarding the variability of hypercarbia in premature infants and how changes over time may relate to neonatal outcomes. There has been significant attention drawn to hypoxia because of the non-invasive oximetry data that are readily available and allow for continuous monitoring and recording. Less focus has been placed on the role of variation and trends in pCO 2 due to the need for invasive collection, and therefore less availability, of such data. The purpose of this study is to describe the trends observed in hypercarbia in a cohort of premature infants born at <32 0/7 weeks GA. From April 2017 to April 2019, we identified all infants admitted to the University of Massachusetts Memorial Medical Center (UMMMC) NICU with a birth GA ≤32 0/7 weeks. Parents of these infants were approached on or after day of life 2 if the infants had a current or previous requirement for positive pressure respiratory support (i.e., mechanical ventilation, non-invasive positive pressure ventilation (NiPPV) or continuous positive airway pressure (CPAP). Informed consent was obtained from parents or legal guardians prior to enrollment. The study protocol was approved by the Institutional Review Board of the University of Massachusetts Medical School. Infants were excluded if they had parents/guardians who were not English or Spanish speaking, had any underlying genetic syndromes, or had been diagnosed with pulmonary disease other than BPD. At UMMMC, all infants born ≤32 0/7 weeks GA are provided noninvasive positive pressure at delivery in addition to any resuscitation as indicated following Neonatal Resuscitation Program guidelines. Those born at <27 0/7 weeks GA are initiated on nasal NiPPV and those 27 0/7 through 31 6/7 are initiated on nasal CPAP (nCPAP). Infants are then weaned or escalated on their respiratory support at the discretion of the clinical team. However, all infants born <32 weeks GA are maintained on CPAP through 32 0/7 weeks postmenstrual age (PMA). In addition, all infants born <32 0/7 weeks GA are started on caffeine therapy within 6 h of birth. 40 Data collection Transcutaneous digital monitors (SDMs) (SenTec, Switzerland) were utilized to prospectively record TcCO 2 levels in consented infants. Twice per week throughout the NICU stay, respiratory therapists placed the transcutaneous SenTec V-Sign Sensor on consented infants immediately after a feeding. The infants were recorded for a period of at least 40 min. This allowed for 20 min of consecutive data to be obtained after filtering for artifact. Data points were recorded once per second by the monitors with a sensor temperature of 41°C. Following each recording, data were downloaded from the SDMs using the V-STATS 4.01 Software. PMA and level of respiratory support were also tracked at the time of each recording. The average TcCO 2 values for each epoch of 20 min were calculated using drift-corrected data. A chart review was performed to extract relevant clinical variables and outcomes. To evaluate the effect of PMA on pCO 2 , a gamma regression model with Generalized Estimating Equation option was used in order to take into account the skewed nature of the distribution of pCO 2 and correlations due to repeated measures of pCO 2 over time. The independent variable, PMA, was categorized into 7 groups: 26-<28, 28-<29, 29-<30, 30-<31, 31-<32, 32-<33, and 33-<45. Logarithmic link function was used, which resulted in rate ratio (RR) pCO 2 of each PMA category compared to the last category (33-<45) as the reference. The model was adjusted for sex, race, birth weight, discharged with or without oxygen, and the level of respiratory support. To analyze pCO 2 based on level of respiratory support, the support types were categorized by conventional ventilation, high frequency ventilation (either oscillator or JET ventilator), NiPPV, CPAP, high-flow nasal canula (1.5 L of flow or greater with varying FiO 2 ), or low-flow nasal canula (1 L/min of flow or less of 100% FiO 2 ). A gamma regression model was performed to exhibit the association between pCO 2 and level of respiratory support. Of the 167 potentially eligible infants during the study period, a total of 54 infants were enrolled, and of these 51 were included in this analysis. Please see Supplemental Material for consort flow diagram (Supplement 1). Infants approached for consent were more likely to have lower birth weight, lower GA at birth, and longer length of stay than those not approached (Supplement 2). Almost all infants received antenatal steroids and the rates of significant morbidities such as severe IVH, necrotizing enterocolitis, and patent ductus arteriosus requiring treatment were low in this cohort ( Table 1 ). The vast majority of infants were managed with non-invasive positive pressure until 32 0/7 weeks PMA. Approximately one quarter of the cohort was diagnosed with moderate-to-severe BPD, and approximately the same number was discharged home on supplemental oxygen. From the cohort of 51 infants, a total of 637 individual 20-min epochs of continuous TcCO 2 recordings were obtained (Table 2) between 26 and 44 weeks PMA. We stratified infants into three groups by birth GA: 24 5/7 to 26, >26 to 29, and >29 to 32 weeks. To test differences in pCO 2 among three groups, F-test for repeated measures yielded power of >0.999 for alpha = 0.05, and 0.996 for alpha = 0.01. The estimated effect size was 0.3 (Supplement 3). The average pCO 2 values for each epoch were calculated using drift-corrected data. Of these recordings, the majority were between 40 and 55 mmHg. To ensure the accuracy of the pCO 2 measurement, we randomly selected one third of the patients in the study and compared pCO 2 values between TcCO 2 and blood gas assessments. Correlation between the two sets of measurements was very high at 0.8 (p < 0.001; Supplement 4). The figure also shows the regression line of PCO 2 on TcCO 2 through the origin. The slope was estimated to be 1.02, which is not statistically different from 1 (p = 0.47), and the regression R-square was as high as 0.97. These are indications of high agreement between two measures. To further demonstrate the agreement, a Bland-Altman plot is shown in Supplemental Material 5. Only two points (2/31 = 6.5%) fall outside of +/−1 standard deviation (SD) from the mean difference between two measures. The paired t test showed that the mean difference of 2.15 (+/−9.13 SD) mmHg between them was statistically insignificant (p = 0.20). In addition to accuracy of the pCO 2 measurement by TcCO 2 , we also investigated the reliability of using 20-min epoch mean of TcCO 2 second-by-second recordings. We randomly selected 25% of the infants from our sample and then randomly selected a total of 192 epochs using a computer algorithm. Using the second-by-second TcCO 2 data, we found that the coefficient of variation (CV) ranged between 0.0019 to 0.33, with a median of 0.02; 95% of CVs were <0.08. CV (=mean/SD) is a measure of the degree of variation. In addition, we calculated the proportion of second-by-second data points that fell between mean ± 1.96 SD. The proportion for the entire sampled epochs ranged from 0.9 to 1, with more than half of the proportions >0.96. Significant changes were noted over time and across PMAs (Fig. 1) . The average CO 2 at younger GAs was 55-60 mmHg but trended to the mid-low 40s by 36-40 weeks PMA. There was a great deal of variability in the measurements. Examining the raw data revealed that there is a clear trend to decreasing pCO 2 with increasing PMA (p = 0.03; Fig. 1 ). This trend remains true examined at different GA categories at birth (Fig. 2a) . There was an increase in variability of pCO 2 in the youngest birth GAs. The mean pCO 2 exhibited a downward trend from early PMA of 26-44 weeks (p < 0.001). The estimated pCO 2 before 32 weeks PMA was in the mid-50 mmHg and was reduced to approximately 45 mmHg at 40 weeks PMA. The rate of "reduction" in pCO 2 is fastest for infants born at older GAs in the first postnatal weeks of life and eventually plateaus in consecutive weeks. The fitted curves and rates of changes over weeks of life are displayed in Fig. 2a, b. Next, pCO 2 assessments were analyzed accounting for the level of respiratory support the infant was receiving at the time of measurement. Infants were managed at the discretion of the clinical neonatologist; there was no specific clinical guidelines specifying level of support for range of pCO 2 . Selective surfactant administration was used per unit guidelines. Invasive mechanical ventilation was used for infants who failed non-invasive support regardless of GA, per the clinical team. The pCO 2 values were reflective of the infants' level of respiratory support at a time of relative stability rather than an independent variable that necessitated a change in support. Our results demonstrate that infants on the highest amount of respiratory support had the highest pCO 2 values, likely reflecting their clinical illness severity (Fig. 3a, b) . As the infants were weaned in their support, our measurements show that their CO 2 did not rebound to higher values but actually decreased over time (Fig. 4) . Infants on room air were more likely to have CO 2 within the normal range compared to those on higher levels of respiratory support, such as NiPPV. It was only for low flow nasal cannula (LFNC) that this trend was not seen. Instead, there was a slight increase in pCO 2 . Although this could be a random variation associated with small sample size, it is possible that this reflects a true difference (Fig. 3a) . Regression analysis of pCO 2 (dependent variable) on level of respiratory support (independent variable) showed that, for the periods receiving high support (NiPPV and high frequency oscillatory ventilation/conventional mechanical ventilation), mean pCO 2 was, 30 35 Post-menstrual age (weeks) 40 45 respectively, 26 and 52% higher than those periods when receiving no support (p < 0.001, Fig. 3b ). For the periods receiving LFNC and CPAP, mean pCO 2 was 14 and 12% higher (p < 0.001). When the cohort was stratified by birth GA category, the mean pCO 2 for the youngest PMA group (26-<28 weeks) was noted to be 24% higher than that of infants at PMA 33-44 weeks (RR = 1.24; 95% confidence interval (CI) 1.00-1.54; p = 0.049). For the next group, 28-<29 weeks, the mean rate is 20% higher (RR = 1.20; 95% CI 1.11-1.30, p < 0.001). For infants born at 29-<30 weeks, the mean rate is 11% higher (RR = 1.11; 95% CI 1.06-1.17, p < 0.001) and those born at 30-<31 weeks GA, the mean rate is 8% higher (RR 1.08; 95% CI 1.04-1.12, p < 0.001). The mean pCO 2 for infants born at ≥31 weeks GA did not vary significantly from the reference of stable values seen in infants' measurements at 33-44 weeks PMA (Fig. 5) . We examined the trends over time of hypercarbia and variation of pCO 2 via TcCO 2 monitoring in a cohort of very premature infants throughout their NICU course. Utilizing newer technology with TcCO 2 monitors, we were able to describe the pattern of ventilation over time and with different levels of support. With this study, we found a pattern of higher pCO 2 in the first weeks of life, regardless of birth GA and level of respiratory support. In the infants born >29 weeks gestation, pCO 2 values rapidly trended to values considered "normal" in healthy subjects (low 40s) and remained relatively stable throughout the remainder of their NICU course. Infants born <29 0/7 weeks gestation required a longer time to achieve pCO 2 levels in the 40s, but on average, infants trended to a stable value by 31-33 weeks PMA, which was not significantly different from that at 36 weeks PMA. Infants born <26 0/7 weeks GA took significantly longer to reach pCO 2 levels in the 40s but ultimately approached this same range. The rate at which the values decreased to the 40s was also different between GA groups; infants at the lowest GAs had the highest pCO 2 values in the first weeks and took the longest time to achieve those stable values seen in babies born at later GAs. It is important to note that the optimal pCO 2 levels for premature infants at different chronological or corrected GA remains unknown. However, it is Fig. 2 Estimated level of TcCO 2 by post-menstrual age and rate of change, using gamma regression model adjusted for level of respiratory support. a Shows both the average level of TcCO 2 as it changes by post-menstrual age and the rate of that change, demonstrating the inverse relationship. Those infants who are the youngest post-menstrual age have the highest average TcCO 2 and then the steepest rate of change. b Shows the same information corrected for respiratory support. reassuring that, off respiratory support, many premature infants did in fact achieve the same pCO 2 levels (low 40s) that are considered healthy in term infants, older children, and adults. Previous studies of ventilation in premature infants have focused on setting pCO 2 goals and then checking at various intervals with blood gas measurement. 22, [24] [25] [26] 41 Respiratory support was then determined by these laboratory values in the setting of a clinical study rather than assessment of infant status. By examining trends of pCO 2 twice each week throughout the course of a NICU hospitalization, our project describes the trends of hypercarbia over the course of postnatal development in a range of GAs. Although the pCO 2 values were highest on the highest levels of support, this also correlated with the most immature, fragile, and critically ill infants. It is more likely that the higher pCO 2 levels were present despite the higher levels of respiratory support, not because of the support level; the hypercarbia likely would have been worse if not for the support provided. Interestingly, the infants did not demonstrate a rebound phenomenon after decreasing the level of respiratory support. This either reflects the fact that higher levels of support promote improvement in lung growth or healing or that the progression to lower respiratory support levels might have been possible earlier than performed in our NICU. The only group where this trend was not seen was in those infants on LFNC. These infants had slightly higher pCO 2 than infants on HFNC and nCPAP. Although the small sample size of this group means that this could be the result of random variation, there is also physiologic plausibility to the hypothesis that it represents a true variation. LFNC does not support ventilation by supporting functional residual capacity and decreasing atelectasis as do both CPAP and HFNC. This trend may be reflective of that decreased support. Other studies have shown that providing distending pressure via nCPAP during periods of lung growth in the NICU support improved pulmonary function later. [42] [43] [44] We believe that the use of gentle non-invasive respiratory support with continuous distending pressure supports lung growth and allows for normalization of ventilation as reflected in our results. Studies randomizing infants to moderately higher pCO 2 targets (permissive hypercarbia) have shown no difference in morbidities between groups, but those targeting even higher levels showed a trend toward increase in rates of IVH and periventricular leukomalacia (PVL). 17, 29, 31, 41 Despite clinical use of permissive hypercapnia and less invasive ventilation, our cohort had extremely low rates of IVH; only 1.9% had severe IVH and no infants were diagnosed with PVL. We found that birth GA and PMA were the most predictive factors determining pCO 2 rather than the level of respiratory support. In this cohort study, there was no mandated respiratory management or explicit pCO 2 targets. Variability among clinical providers was therefore likely. However, the data suggest that time (and growth), rather than ventilatory strategy is what ultimately led to greater respiratory stability. Previous studies have shown that lung growth continues in the postnatal period and is correlated with somatic growth. [45] [46] [47] Conversely, fetal growth restriction has been associated with poor lung growth and function both in human and animal models. 1, 45, 46, [48] [49] [50] [51] It is possible that lung growth with increasing age resulted in increase in alveolar surface area and thinning of the interstitium, allowing for improved gas exchange. To our knowledge, this is the first prospective, longitudinal cohort examining variation of pCO 2 in premature infants during their NICU hospitalization. Previous research has relied on blood gas assessment of PaCO 2 , with blood gases obtained at the discretion of the clinical team. This study describes the trends observed during periods of both clinical stability and instability. TcCO 2 monitors are still a newer technology. To validate our data, we reviewed study participants' medical charts and found excellent correlation between transcutaneous values and capillary blood gas values obtained within an hour of the measurement (see Supplementary Material 4 and 5). In addition, our results are consistent with what was observed in the trials by Thome and Ambalavanan, that highest values of pCO 2 are markers of respiratory illness severity, which in this population is most determined by birth GA and PMA. [24] [25] [26] A limitation of this study is the potential incorrect assumption that the infants' ventilation status during the two 20-min epochs recorded each week accurately represents the infants' status for the other approximately 167 h each week. It is possible that there was significant variability that was not captured in our data. The trend that emerges, then, is even more striking, as more limited discrete data would be more prone to random variability. Another limitation is the smaller sample size of those infants born at <26 weeks GA. Given the known respiratory morbidity risk in this population, further evaluation of this vulnerable group of infants is needed. Finally, this is a single-center study so the hypercarbia trends reflect our unit's respiratory practices and may not be generalizable. The number of recordings and the longitudinal nature of the data spanning virtually the entire NICU course until discharge is a strength of this study. The finding that most of the preterm infants in our cohort, once off respiratory support, can eventually achieve and maintain pCO 2 levels that are considered normal and healthy in older heathy populations (i.e., low 40s) is an important finding. While the timing to achieve this may reflect our individual unit's respiratory practice, it demonstrates that these levels are achievable; correlation of these levels with other health outcomes is now needed. In conclusion, infants born at earlier GAs have significantly higher pCO 2 values in the first weeks of life despite being on higher levels of respiratory support. These values trend toward levels considered normal in other populations (low 40s) by 31-32 weeks PMA regardless of the birth GA. Further work is needed to better describe the variability of pCO 2 , particularly for those infants born <29 weeks GA and in the first weeks of life and how the degree of variability may influence later outcomes. 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SenTec loaned several transcutaneous carbon dioxide monitors for use in this prospective cohort study. Each author has met the Pediatric Research authorship requirements. All authors made substantial contributions to the conception and design, acquisition of data, and/or analysis and interpretation of data. K.P.S. drafted the article. The online version of this article (https://doi.org/10.1038/s41390-020-01308-2) contains supplementary material, which is available to authorized users.Competing interests: None of the authors have any financial ties to the products used in the study.Consent statement: Informed consent was obtained from the parents or guardians prior to enrollment.Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.