key: cord-0012663-wgw5h8n3 authors: Ouattara, Mahamoudou; Tamboura, Mamadou; Kambire, Dinanibe; Sanou, Mahamoudou; Ouattara, Kalifa; Congo, Malika; Kaboré, Adama; Sanou, Soufiane; Kabré, Elie; Sharpley, Sable; Tran, Theresa; Schwartz, Stephanie; Ouangraoua, Soumeya; Ouedraogo, Abdoul-salam; Sangaré, Lassana; Ouedraogo-Traore, Rasmata; Whitney, Cynthia G.; Beall, Bernard title: Identification of Streptococcus suis Meningitis by Direct Triplex Real-Time PCR, Burkina Faso date: 2020-09-03 journal: Emerg Infect Dis DOI: 10.3201/eid2609.200203 sha: 09b5e1a8e7cb32c8e169bdbc96ed59d017ca6899 doc_id: 12663 cord_uid: wgw5h8n3 Meningitis confirmation in Burkina Faso uses PCR for detecting Streptococcus pneumoniae, Neisseria meningitidis, or Hemophilus influenzae. We identified 38 cases of meningitis among 590 that were PCR-positive for 3 nonpneumococcal streptococcal pathogens, including 21 cases of Streptococcus suis. Among the country’s 13 regions, 10 had S. suis–positive cases. Burkina Faso conducts nationwide case-based surveillance for bacterial meningitis. Cerebrospinal fluid (CSF) samples collected from patients with suspected meningitis are sent to 1 of 5 national laboratories for confirmation by culture and real-time PCR testing (6) . Because of multiple challenges encountered by the laboratories in isolating bacteria from CSF, the confirmation of meningitis cases relies heavily on molecular detection (6) . The real-time PCR assay currently used at the national laboratories only detects Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae. Between 2015 and 2018, the national laboratories tested 7,174 CSF samples from patients with suspected meningitis nationwide. Of these, 4,930 (68.7%) were PCR-negative for S. pneumoniae, N. meningitidis, and H. influenzae. To further investigate suspected meningitis cases that were PCR-negative after testing for the 3 pathogens, a subset of specimens was selected for additional screening based on leukocytes counts >50 cells/mm 3 . In total, 912 PCR-negative specimens were available for the study; among these, 590 fit the selection criteria. The specimens were retested by using a triplex direct real-time PCR that was designed for the simultaneous detection of Streptococcus agalactiae (group B Streptococcus [GBS]), S. pyogenes (group A Streptococcus [GAS]), and S. suis. The nucleotide sequences and final concentrations of the primers and probes used in this assay (Table 1) were identical to those used in singleplex real-time PCR methods (7) (8) (9) (10) , with the exception that FAM was replaced by HEX as the reporter dye for cfb-specific probes for the GBS target and by Cy5 as the reporter dye for spyspecific probes for the GAS target. Each reaction was prepared in a final volume of 25 µL, including 1 µL of each primer and probe, 2 µL of CSF as DNA template, 12.5 µL of PerfeCta MultiPlex qPCR ToughMix mastermix (QuantaBio, https://www.quantabio.com), Meningitis by Direct Triplex Real-Time PCR, Burkina Faso and 1.5 µL of PCR-grade water. The thermal profile for the real-time PCR runs was 1 cycle of 55°C for 5 min, 1 cycle of 95°C for 10 min, then 40 cycles of 95°C for 15 s and 60°C for 1 min. All specimens were tested by real-time PCR for the presence of the human ribonuclease P (RNaseP) gene to check for the presence of inhibitors. A specimen was considered positive if the cycle threshold (C t ) for 1 of the bacterial targets was <35 and was considered negative if the C t was >40 for all bacterial targets with C t <35 for RNaseP. If C t was between 35 and 40 for the bacterial targets and <35 for RNaseP, the specimen is retested; if the result was reproducible, it was considered positive. The lower limits of detection (LLDs) were determined as previously described (11) . The LLD of the direct triplex assay (3 CFU/µL for S. suis, 28 CFU/µL for GBS, and 26 CFU/µL for GAS) were compared with the LLD of the triplex assay conducted on DNA extracted from the same serial dilutions (2 CFU/µL for S. suis, 5 CFU/µL for GBS, and 3 CFU/µL for GAS). Among the specimens tested, 21 were positive for S. suis, 13 for GAS, and 4 for GBS (Table 2) . Of the 21 S. suis-positive case-patients, 1 was 4 years of age, 3 were 5-14 years of age, 2 were 15-29 years of age, 9 were 30-49 years of age, and 6 were >50 years of age; 16 (76.2%) were male and 5 (23.8%) were female. Two (9.5%) patients died, 14 recovered, and the outcome for 5 was unknown. Eight (61.5%) of the GAS-positive case-patients were <5 years of age and 5 were 5-14 years of age; 1 (7.7%) patient died, 5 recovered, and the outcome for 7 was unknown. All the GBS-positive case-patients were <6 months of age and all recovered. Median time from symptom onset to CSF collection was 2 days (range 1-10 days) for all the patients. S. suis-positive cases were found among specimens from rural districts in 10/13 regions of the country with 28% (6/21) from the Centre-Est Region and 19% (4/21) from the Plateau Central Region. All adults with S. suis were farmers. Because of the retrospective detection of the etiology, the extent of any direct exposure of the S. suis-positive patients to pigs or pork products was unknown and attempts to collect additional information from the patients or their families were unsuccessful. (7) Small-scale pig farming is a major economic activity in most parts of Burkina Faso. Although porcine-related occupations, undercooked pork consumption, and exposure to pigs have been identified as major risk factors for S. suis infection (5), its occurrence in a wide range of animal species have been reported (2) . DNA extracted from the S. suis-positive specimens were used for a multiplex conventional PCR for serotype detection (12) . All the specimens were serotype 2 or 1/2 because the method used here could not clearly differentiate serotypes 2 and 1/2. Bacterial isolates from these cases were not available for further characterization. Serotype 2 is believed to cause 74%-95% of human cases of S. suis infections reported worldwide, but serotype 1/2 has not been reported to cause disease in humans (1, 2) . Limited data are available regarding the incidence of streptococcal infections in Africa, and the disease burden likely is underestimated, especially for disease caused by S. suis. Two reported studies conducted in Togo identified 16 human cases of S. suis meningitis during 2010-2015 caused by serotype 2 (13, 14) . Another recent study in Madagascar described 2 human cases of S. suis meningitis, also caused by serotype 2 (15) . Our study reports 21 cases of S. suis meningitis from 2015-2018 in Burkina Faso. S. suis infections more commonly are observed in adult males and are directly correlated with occupational exposure to pigs or pork products (2, 13, 15) . All adult cases reported here were farmers, although their exposure to pigs or pork was unknown. The presence of children among the patients in this study and in previous studies (13) indicates that older children also should be considered at risk. Finding 21 cases of this outbreak-prone zoonotic pathogen in this study raises concern about the incidence of S. suis disease in Burkina Faso and in other parts of Africa where pigs are raised. Prospective surveillance for S. suis could help identify farming communities where measures to prevent disease and its potential socioeconomic damages are needed. Dr. Ouattara is a microbiologist in the Streptococcus Laboratory, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. His primary research interests include meningitis, invasive streptococcal diseases, bacterial pathogenesis, and global laboratory capacity building. Streptococcus suis, an important pig pathogen and emerging zoonotic agent-an update on the worldwide distribution based on serotyping and sequence typing Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I-epidemiology Streptococcus suis: a new emerging or an old neglected zoonotic pathogen? Septic shock caused by Streptococcus suis: case report and investigation of a risk group Streptococcus suis meningitis in adults in Vietnam MenAfriNet Consortium. 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