key: cord-0009086-46vfnxyv authors: nan title: Expression systems date: 2004-08-26 journal: Curr Opin Biotechnol DOI: 10.1016/0958-1669(92)90088-z sha: 1cdd50c17b5da06c8ce347a9c9a6e161ce104faf doc_id: 9086 cord_uid: 46vfnxyv nan Encoding a Xylanase and an Endo-bcta- (1, 3 Biologically Active Bcta-Subunit of Human Nerve Growth Factor in Escherichia-ColL Gene Nueleotide Sequences of the Acinetobacter-Calcoaceticus BenABC Genes for Benzoate 1,2-Dioxygenasc Reveal Evolutionary Relationships Among Multicomponent Oxygenases Expression of the Aphthovirus RNA Polymerase Gene in Escherichia-Coli and Its use Together with Other Bioengineered Nonsteuctural Antigens ill Detection of Late Persistent Infections MaYtmi~*~g the Expression of a Recombinant Gene in Escherichia Coli by Manipulation of Induction Time Using Lactose as Inducer Efllux-Medlated Multidrug Resistance in Bacillus-Subttlis -Stmtlaeities and Disslmi!~riti©s with the Mammnli~n System Molecular Cloning and Nueleotidc Sequence of a Pectin Lyase Gene from Pseudomonas-Marginalis N6301 Cloning and Characterization of Genes Responsible for Metabolism of Nitrile Compounds from Pseudomonas-Chlororaphis B23 Cloning of the Hnman Erythropoietin Receptor Gene Isolation and Characterization of Plasmids from Different Strains of Methanobacteriom-Thermoformicicum Site-Specific Integration of Genes into Hot Spots for Recombination Flanking Aad& in To21 Transposons Glutamyl-Transfer RNA Synthetase from Thermus-thermophllus HB8 -Molecular Cloning of the GltX-Gene and Crystallization of the Overproduced Protein Cloning of Nltrogenase Structural Genes from the Obligate Methanotroph Methyiococcus-Capsulams (Bath) Production in Escherichia-Coli of Porcine Type-I Collagenase as a Fusion Protein with Beta-Galactostdase Distribution, Purification, and Characterization of Thermostable Phenylalanine Dehyds-ogenase from Thcrmophillc Actinomycetes Genetic Improvement of Escherichia-Coli for Ethanol Production -Chromosomal Integration of Zymomonas-Mobilts Genes Encod~g Pyruvate Decarboxylase and Alcohol Dehydrosenase-H Agrobacterimn-Rhizogenes Lar.Z-rolC Germ Expression in Escherichia-Coli -Detection of the Product in Transgenic Plants Using RolC-Specific Antibodies Design, Chemical Synthesis, and Expression of Genes for the Three Human Color Vision Pigments Suppression of an Eseherichia-Coli SecAts Mutant by a Gene Cloned from Staphylococcus-Carnosus Characterization and Functional Expression of a CDNA Encoding Egasyn (Esterase-22) -the Endoplasmic Reticulum-Targettng Protein of Beta-Glucuronidase High Level Heteroiogous Expression In E Co//Using the Anaerobicallyactivated ntrB Promoter Growth Hormone Releasing Factor Analog Expression hi Saceharomyces-Cerevtstae Fermentation of a Yeast Producing A. Niger Glucose Oxidase: Scale-up, Purification and Characterization of the Recombinant Enzyme Construction of Yeast Artificial Chromosome (YAC) Clone Banks Covering Three Genome Equivalents and Isolation of YACs Containing the Human Gene Encoding Tumor Necrosis Factor Receptor.bern Effmient Synthesis of • Secreted Murine Interleuktn-2 By Saccharomyces Cerevtstae --Influence of 3'-Untranslated Regions and Codon Usage Expression of Mammal|art Tyroshie Amtnotransfe£asc in 8accharomyces-Cerevislae and Eschcrlchla-coli -Purification to Homogeneity and Characterization of the Enzyme Overproduced hi the Bacteria Expression hi Yeast of a CDNA Copy of the K2 Killer Toxin Germ Expression of Human Antithrombhi-IH hi the Cellular Slime Mould Dictyostellm-Discoideum Clolting Ht,m~n Pyrrollne-5-Carboxylate Reductase CDNA by Complcmentation hi Saccharomyces-Cerevisiae Consttactlon of Two Stable Bifunctional Plasmids for Streptomyces Spp and Escheriehla.colL FEMS Mtcrobiol Lett Production of Functional IgM Fab Fragments by Saccharomyces Ceresisla¢ Site-Specific O-Glycosylation of Human Graaulocytc/Macrophage Colony-Stimulating Factor Secreted by Yeast and An|m~l Cells NucleiC Acid Bhidiug Properties of the 92-kDa Repllcase Subuuit of Alfalfa Mosaic Virus Produced in Yeast High-level Secretion of Correctly Processed Recombinant Human Intcrleukin-1-beta hi Kluyveromyees Lactis Stable Multtcopy Vectors for Higillevel Secretion of Racombhiant Human Serum Albumin by Kluyveromyces Yeasts Opthnization of the Mitotic Stability of PKD1-Based Expression Vectors. 4th Meeting on Biology of Kluyveromyces Genes Encoding Peroxlsomal Enzymes are not Necessarily Assigned on the Same Chromosome Of an Normal-Alkane-Ufllt~hle Yeast Candlda-Tropicalis Characterization, l~pression hi 8treptomyces-Lividans, and Processing of the Amylase of Streptomyces-Griseus IMRU-3570 -Two Different Amylases are Derived from the Same Gene by an Intracellular Processing Mechanism Improved Method for I41~h l~fi~cicncy Transformation of intact Yeast Cells Ioni~ and in Vivo Expression of the Human GART Gene Using Yeast Artificial Chromosomes Development of a New Host Vector System 111 Synthesis of H,lm~*~ Initiation Factor-2alpha hi Saccharomyces-Cerevisiae ConstruL~Jon and Characterization of a Yeast Artificial Chromosome Library of Arabidopsis Which is Suitable for chromosome Walldng Polyethylene Glycol-induced Internalization of Bacteria into Fungal protoplasts -Electron Microscopic Study and Optimization of Experimental Conditions Production and Purification of Recombinant Human Interleukin-6 Secreted by the Yeast Saccharomyces Cerevisiae Entire Maize Chloroplast Genome is Stably Maintained in a Yeast Artificial Chromosome Messenger RNA Splicing hi Yeast -Clues to Why the Spliceosome is a Ribonucleoprotehi Xylltol Production by Recombinant Sacchromyces Cerevisiae. Btotechnology Identification of a Tomato Gene for the Ethylene Forming Enzyme by Expression hi Yeast Characterization of Recombinant Bat Cathepshi-B and Nongiycosylated Mutants Expressed in Yeast -New Insights into the PH Dependence of Cathepshi-B-Catalyzed Hydrolyses Transcriptional Control of Yeast Phosphofructokinase Gcmc Expression Targets for Cell Cycle Arrest by the Immuanosuppressant Rapamychi hi Yeast Mutations of the Alpha. Galactostdase Signal Peptide Which Greatly Enhance Secretion of Hcterologous Proteins by Yeast Functional Expression of Rat M5 Muscarinic Acctylchollne Receptor hi Yeast Point • Mutations Upstream of the Yeast ADH2 Poly(A) Site Significantly Reduce the Efficiency of 3"-End Forlnation Production and Purification of Recombinant Hnmam Interleukin-5 fromYeast and Bacnlovirus Expression Systems The Direct Cloning of the Yeast Genome Using the Gap-Fllllng Method and the Complete Physical Mapping of Saccharomyces-Cerevisiae ChromosomeoVL Gene Stable Synthesis of Viral Protein-2 of Infectious Bursal Disease Virus hi Saceharomyces-Cerevislae Simditaneous Expression of the S and L Surface Antigens of Hepatitis B, and Formation of Mixed Particles hi the Mcthylotrophtc Yeast Introduction of Terpenc-producing Ability hi a Whie Strain of Saccharomyces Cerevislae Inhibition of Cell Proliferation hi Saccharomyces-cerevisiac by Expression of Human NAD+ ADP-RtbosyBa-ansferase Requires the DNA Binding Domain (Zinc Fingers) An Apparent Rare Codon Effect on the Rate of Translation of a Neurospora Gene Methanol as a Convenient Inducer of Heat Shock Element-Directed Hetcrologons Gene Expression in Yeast Coostraction and Transductinn of a Shuttle Vector Bearing the Cos Site of Streptomyces Phage-PhlC31 and Determination of Its Cohesive Ends Evaluation of Foreign Gene Codon • Optimization in Yeast: Expression of a Mouse IG Kappa chain The use of a Synthetic TRNA Gene as a Novel Approach to Study in Vivo Transcription and Chromatin Structure in Yeast Functional Expression of • Human mdrl in the Yeast Saccharomyces Cerevisiae Yeast Artificial Chromosome Libraries Containing Large Inserts frOnl Mouse and Human DNA Expression of RNase P RNA in Saecharomyces Cerevisiae is Controlled by an Usual RNA Polymcrase HI Promoter Secretion of N-gly~osylated Interleukin-1 Beta in Saccharomyces Cerevisiae Using a Leader Peptide from Candida Albicans. Effect of N-Linked Glycosylation on Biological Activity Interaction of Trans and Cis Regulatory l~lements in the INOI Promoter of Saccharomyces Cerevtsiae isOlatiOl~ Charactertsation and Molecular Cloning of New Mutant Alleles of the Fission Yeast P34cdc2+ Protein KInase Gene -Identification of Temperature-Sensitive G2-Arresting AIleles ~t: improved Shuttle Vectors for Cloning and High-Level Cu2+-Mediated Expression of Foreign Genes in Yeast Direct Transfer of Plasmtd DNA from Yeast to E. COli by I~lectroporation Molecular Cloning of Aeetyi Coenzyme-A -Deacetylcephalosporin-C O-Acctyltransferase CDNA from Acremonium-Chrysogenum -Sequence and Expression of Catalytic Activity in Yeast Structure, Expression, and Antisense Inhibition of the System in Precursor Gene The K2-Type KiLler Toxin-Encoding and Immunity-Encoding Region from Saccharomyces-Cerevislae . Structure and Expression in Yeast Breeding of a Distiller's Yeast by Hybridization with a Wine Yeast Production of Proteins by • Secretion from Yeast A Yeast Artificial Chromosome Contig Containing the Complete Duchenne Muscular Dystrophy Gene Cloning and Characteristics of a Positive Regulatory Gene, THI2 (PHO6), of Tbi~ml Complementation of the Cs Dis2-11 Cell Cycle Mutant of Sehizosaccharomyees Pombe by a Protein Phnsphatase from Secretory Expression of the Human Seruln Albnmin Gene in the Yeast Isolation and Characterization of Two Biologically Active O-Glycosylated Forms of Human Parathyroid Hormone Produced in Saccharomyces-CcrevisLae -Identification of a New Motif for O-Glycosylation Expression and Secretion of Staphylococcal Nuelease in Yeast -Effects of Amino.Terminal Sequences A Novel Highly Regulated, Rapidly inducible System for the Expression of Chicken Progesterone Receptor, C2PRA~ in Saccharomyce$ Cerevisfae A Twin-Reporter Vector for Simultaneous Analysis of Expression Signals of Divergently Transcribed, Contiguous Genes in Filnmentous Fungi An Androgeninducible Expression System for Saccbaromyces Cereviaiae Cloning Of the HIA C1ass-ll l~gion in Yeast Artificial Chromosomes An impgoved Transformation Vector for the LIgnin-Degrading White Rot Basidomyecte Phanecochaete-Chrysosporium Complementation of • the Saccharomyces Cerevisiae Srbl-1 Mutation --an Autoselection System for Stable Plasmid Maintenance Expression of Bacterial Mercuric Ion Reductase hx Saccharomyces-Ce2evtsiae Mouse ~dmonary Cytochrome-P-450 Naphthalene Hydroxylase -CDNA Cloning, Sequence, and Expression in Saccharomy~es-Cerevtsiae Recombinant Bordetella Pertussis Pertactin (P69) from the Yeast PIchia Pastoris: High-level Production and Immunological Properties Expression of Tetanus Toxin Fragment C in Yeast: Gene Synthesis is Required to l~ltmtoate Fot'mitotm Polyadenylation Sites in AT-Rich DNA~ Ionin~ Sequencing and Mutat~nal Analysis of the Cytochrome-c552 Gent (cycB) from Bradyrhizobtum-Japonicum Strain-110 Direct Introduction and Transient Expression of Capped and Non Capped RNA in Saccharomyces Ccrevisiae Enhanced Secretion of Human Nerve Growth Factor from Saccharomyces Cerevisiae Using an Advanced Delta-integration System Isolation and Structural Analysis of the Laccase Gene from the IAgntn-Degrading Fungus phlebla-Radtata, f Gen Microbio11991 Expression of Proteins and Glycoprotcins Encoded by the Haploid Nuclei in the Dtkaryotic State in the Bastdiomycete Agrocybe-Aegertta Vectors for • Constitutive and Inducible Gene Expression in Yeast Integration of DNA-fragments by Illegitimate Recombination in Saccharomyces Cerevislae Production of Hepatitis B Surface Antigen (PreS2 + S) by Highcell Density Cultivations of a Recombinant Yeast Synonymous Codon Usage in • Saccbaromyces Cerevisiae. Yeast Yeast Artificial Chromosome Vectors for Efficient Clone Manipulation and Mapping~ Genomics Gene Expression in Yeast: Protein • Secretion. Curr Optn Btotechno11991 Selection of Secretory Protein-Encoding Genes by Fusion with PHO5 in Saccharomyces-Cerevisiae New Shuttle Voctors for Direct Cloning in Saccharomyces-Cerevisiae Vec$ors for Inserting Selectable Markers in Vector Arms and Human DNA Inserts of Yeast Artificial Chromosomes (YACs) Cloning and characterization of a Gene Which Determines Osmotic Shock Stability in Saccharomyces Cerevisiae Construction and Analysis of Recombinant Glucanolytic Brewer's Yeast Strains Folding Mechanism of Mutant H.mu. Lysozyme C77/95A with increased Secretion Efficiency in Yeast Structure of Yeast PGKL 12&kDa Killer-Toxin Secretion Signal Sequence -Processing of the 128-kDa Killer-Toxin-Secretion-Signal -Alpha-Amylase Fusion Protein Coexpression of Human CAMP-Specific Phosphodiesterase Activity and High Af~ntty Roilpram Binding in YeasL JBtol Chem Characteristics of Nlcottana-tabacum Nitrate Reductase Protein Produced in Saccharomyees-cerevislae OpttmiTatkJn of Pro-urokinase Secretion from Recombinant Saccharomyces Cerevtstae Isolation of Nuclei from Aspcrgillns-nidulans Protoplasm Development of a Strain of Hansenula Polymorpha for the Efficient Expression of Guar a-Galactosidase Hcterulogous Gene Expression in the • Yeast Saccharomyces Cerevisiae Efficient Expression of Bovine Alpha-Lactalbumin in Saccharomyces-Cerevtstae A Cell-free Extract • from Yeast Cells for Studying Messenger RNA Turnover Turnover Rate of Yeast pgk Messenger RNA cart be Changed by Specific Alterations in Its l~-atler Structure Purification and Characterization of Recombinant Tissue Kallikrein from Escherichla-Coli and Yeast Identification of the DNA Binding Site for Ngfi-B by Genetic Selection in Yeast Ex~ressiDn and Functional Study of Wild-Type and Mutant H.man Cytochrome-P450c21 in Saceharomyces-Cerevisiae Targeted Inactivation of the Insulin Receptor Gene in Mouse 3T3-L1 Fibroblasts Via Homologous Recombination Cloning of a G1ycine Receptor Subtype Expressed in Rat Brain and Spinal Cord During a Specific Period of Nenronal Development A Pair of Seleetable Herpesvirus Vectors for Simultaneous Gene Expression in Human Lymphoid Cells Ftmctioual Aatlalysis of Defined Mutations in the Immunoglobulln Heavychain Enhancer in Transgenic Mice A Shuttle Vector for Analysis of Illegitimate Rerombination in Mamm*!taq Ceils -Effects of DNA Topoisomerase Inhibitors on Deletion Frequency A chimeric • • Mamm~lt~q Tratma~dArator Based on the Lac Repressor that is Regulated by Tempermturc and Isopropyl ~-D-Thiogalactupyranoside Systemic Delivery of Recombinant proteins by Genetically Modified Myoblasts Itish-Level Expression of a Recombinant Antibody from Myeloma Ceils Using a Glutamine Synthetase Gene as an Amplifiable Selectable Marker Efficient CDNA Cloning by Direct Phenotypic Correction of a Mutant Hnrn~n Cell Line (HIrRT-) Using an Epstein-Barr Virus Derived CDNA Expression Vextur lllduition of a Mutant Phenotype in Human Repair Proficient Cells after Ovcr~preasion of a Mutated Human DNA Repair Gene The Rodent B2 Sequence can Affect Expression when Present in the Trans~d Region of a Reporter Gene Characterization of an Adult Muscle Aretylcholine Receptor Subunit by Expression in Elbroblasts Identification of Protein-Binding Scquences Mediating Constitutive And 12-O-Tctradecanoylphorbol-13-Acetate-induced VL30 Transcription in Cultured Mouse and Human Keratinocytes Human and El,m~n -Yeast ~C U6 SnRNA Genes Identify Structural. l~lements Required for Expression in Yeast The Mitochondrial Un¢oupltug • Protein in Brown Fat: Correlation Between DNase I Hypersensitivity and Expression in Transgenic Mice The Expression of Interlcukin-6 by a Rat Macrophage-Derived CeII Line Use of Urokinase-type Plasminoguen Activator Genc as a General Tool to Monitor Expression In Transgcnic Animals: Study of the Tissue-specificity of the Murine Whey Acidic Protein (WAP) Expression Signals M: A Surface Expression Vector for Antibody Screening Toxicity, Mutageniclty, and Mutational Spectra of N-Ethyl-N-nitrosourea in Human Cell Lines with Different DNA Repair Phenotypes Exon Amplification -a Strategy to Isolate Mammal|an Genes Based on RNA Splicing Expression of Recombinant IInm~n Plasminogen in Mo*nmoli0~ Cells is Augmented by Suppression of Plasmin Activity Regulation of the IInm~n ApOA-H Gene by the Synergistic Action of Factors Binding to the Pro~mmal and Distal Regulatory Elements Direct Apical Sorting of Rat Liver Dip'~ptidylpcptidasc-IV Expressed in Madin-Darby Canine Kidney Cells Parental-• specific Mcthylation of an Imprinted Transgene is Established During Gametogenesis and progressively Changes During Embryogenesis Transforming Function of Proto-ras Genes Depends on Hcterologous Promoters and is Enhanced by Specific Point Mutations Effect of intron a from Hmman Cytomegalovtrus (towne) Immediate-early Gene on Heterologous Expression in M~mm~li~m CellS Stimulation of Protein Synthesis in COS Cells Transfected with Variants of the Mpha-Subunlt of Initiation Factor ELF-2 Rctroviral Expression of Recombinant P47(phox) protein by Elmtein-Barr Virus-Transfo~ Lymphocytes-B from a Patient with Autosomal Chronic Granulomatous Disease Mice Lacking MHC Class II Molecules Screening for B~eeeptor Ltgands Using Large Libraries of Peptides Linked to the C-Terminus of the Lac Repressor Molecular Cloning, Chara~erizatlon, and Genomic Localization of a Human Potassiuln Channel Gene Regulatory Regions of Rat Insulin I Gene Neeessary for Expression in Trm0sgenic Mice The Receptor for Ciliary Neurotrophic Factor Induction of Expression of the Alphavbetal and Alphavbeta3 Integrin Heterodimers During Rctinoic Acid-Induced Neuroual Differentiation of Murtne Embryonal Carcinoma Cells Inhibition and Enhancement of Eukaryotic Gene Expression by Potential Non-B DNA Sequences ~tion and Enhancement of Eukaryotic Gene Expression by Potential Non-B-DNA Sequences Protection Against Lethal Cytomegalovirus Infection by a Recombinant Vaccine Containing a Single Nonameeic T-Call Epitop¢ High-Efficiency Protein Synthesis from T7 RNA Polymerase Transcripts in 3T3 Elbroblasts Recombinant Baculoviruses Expressing Yellow Fever vires-E and NS1 Proteins Elicit Protective Immunity in Mice Systemic Delivery of I~l,m~n Growth Hormone by Injection of Genetically Engineered Myoblasts Tracheal Antimicrobial Peptide) a Oysteine-Rich Peptide from M~mmalia~ Tracheal Mucosa -Peptide Isolation and cloning of a CDNA Gene Transfer into Normal Hummt Hematopoietic Cells Using in Vitro and in Vivo Assays Transgenic Production of a Variant of Human Tissue-type Plasminogen Activator in Goat Milk: C~neratton of Transgenic Goats and Analysis of Expression A Novel Cationic IAposome Reagent for Efficient Transfection of Mammalian Cells LcukemLa Inhibitory Factor Receptor is Structurally ReLated to the IL-6 Signal Transducer, Gp130 The Encephalomyocarditis Virus internal Ribosome Entry Site Allows Efftcient Coexpression of Two Genes from a Recombinant Provirus in Cultured Cells and in Embryos Baculovirus-Expressed Glycoprotein-H of Herpes Simplex Virus Type-1 (HSV-1) induces NeutralD2mg Antibody and Delayed Type Hypersensitivity Responses, But Does not Protect Immunized Mice Against Lethal HSV-1 Challenge A Vacelnia Virus Double Recombinant Expressing the F-Genes and H-Genes of Rinderpest Virus Protects Cattle Against Rinderpest and Causes no Pock Lesions • Regulatory Elements that Control Lineage-specific Expression of MyoD Tight Control of Gene Expression • • in Mammaliaq Cells by Tctracycline-respousive Promoters Expression of Biologically Active Heterodimeric Bovine Follicle-stimulating Hormone in Milk of Transgenic Mice Construction of a I-DIm~ Shuttle VeCtor Contatnin~ a Sin~e Nitrogen Mustard Inters•rand, DNA-DNA Cross-Link at a Unique Plasmtd Location Mammal|aq Expression of the Human Sex Steroid-Binding Protein of Plasmm (SBP or SHBG) and Testis (ABP) -characterization of the Recombinant Protein Expression and Characterization of Recombinant Human Acyloxyacyl Hydeolase, a Leukocyte Enzyme that Deacylates Bacterial Lipopolysacrharides Inhibition of Moloney • Murtne Virus-induced Ig0kemia in Transgenic Mice Expressing Antis•rise RNA Complementary to the Reteoviral Packaging Sequences Importance of Globin Gene Oeder fur Correct Deveiopmental Expression The 5'-Flanking Region of the Hi,ms. CGL-1/Granzyme-B Gene Targets Expression of a Reporter Gene to Activated Lymphocytes.T in Transgenic Mice TWO DiStinct FuOctional High • ~ Af~xtity Receptors for Mouse Interleuktn-3 (IL-3). EMBOJ1992 The Length of Homology Required for Gene Targeting in Embryonic Stem Cells Highly inducible Synthesis of Heterologous Proteins in Epithelial Cells Carrying a Glucocorticold-Responsive Vector New Vector Delivers Genes to Lung Cells ~F~Rorial Mulli-snbunit Proteins on the Surface of Filamentous Phage: Methodologies for Dlsp~ring Antibody (Fab) Heavy and L~ht Chains In Vitro Transcription of Baculoviras Immediate Early Genes: Accurate MRNA Initiation by Nuclear Extracts from Both Insect and I~nm~q Cells Molecular Mechanism of Tissue-Specific Regulation of Mouse Rentn Gene Expression by CAMP -Identification of an Inhibitory Protein that Binds Nuclear Transcriptional Factor A Modified Procedure for Replica Plating of Mammalian Cells Allowing Selection of Clones Based on Gene Expression Regulation of Basal and Luminal Cell-Specific Cytokeratin Expression in Rat Accessory Sex Organs -Evidence for a New Class of Androgen-Repressed Genes and Insight into Their Palrwise ControL fBiol Chem Targeting the Eseherichta-Coll Lac Repressor to the M~mmalia~x Cell Noel•us Early induction of Ribonucleotide Reductase Gene Expression by Transforming Growth Factor-betal in M~dignant H-ras Transformed Cell Lines inactivation of Exogenous Beta-Lactamasc in Transformed Yeast Saccharomyces Cerevisiae LOW Level of Hox1.3 Gene Expression Does not Preclude the use of Promoterless Vectors to Generate a Targeted Gene Disruption induction ofCypla-I Dinxin-responsive Enhancer in Transgenic Mice Generation and • Analysis of Interleukin4 Deficient Mice Cloning of a Growth Arrest-Specific and Transforming Growth Factor Beta-Regulated Gene, TL1, from an Epithelial Cell Line Molecular Cloning and Sequencing of the Gene for a Halophlllc ~lkaltne Serine Protease (Halolysin) from an Unidentified Halophtiie Arehaea Strain (172P1) and Expression of the Gtme in Raloferax-VolcantL JBacterio11992 Genetic Characterization of Human Imm,modeficiency Virus Type-1 Nef Gene Products Translated Invitro and Expressed in W~mm~lt~n Ce~. f V~ro11991 Development of a high-expression Vector A Novel Method for Generating stable E~h-Level Transfectants Involving Direct lmmllnomagnetic Selection for Cell-Surlace Epitopes -Expression of HIA Cluss-H Genes in HeLa Ceils A Novel Method for Generating Stable High-Level Transfectants L~vohring Direct Immuno~etic Selection for Cell-Surfaee Epitupes -Expression of HIA Class-II Genes in HeLa CeiLs Improved Vectors for Stable Expression of Foreign Genes in Mammaltaq Cell~ by use of the Untranslated Leader Sequence from EMC Virus Role of Carbohydrate Modification in the Production and Secretion of Human Grsnulocyte Macrophage Colony-Stimulating Factor in Genetically Engineered and Normal Mesenchymal Cells Localization of TIMP in Cye~g Mouse Hair Expression of a Cloned C~mm~-Amittobutyric Acid Transporter in M~m~l;~n Ceils Transgenie Mice that Express a Mini-Gene Version of the Human Gene for Type-I ProcoBagen (COL1A1) Develop a Phenotype Resembling a Lethal Form of Assessment of Virus Production and Chloramphenicol Acctyl Transferase Expression by Insect CelLs in Serum-Free and Serum-Supplemented Media Using a Temperature-Sensitive Baculovirus Expression • • Cloning of the Htlman IL-3 Receptor CDNA Reveals a Shared ~Subunit for the Human IL-3 and GM-CSF Receptors Purification of Recombinant Human Prostromelysin -Studies on Heat Activation to Give High.Mr and Low-Mr Active Forms, and a Comparison of Recombinant with Natural Stromelysin Activities Expression of Recombinant Calf Prochymosin in M~mm~ltan Cell Cnkure Apollpoprotein(a) -Expression and Characterization of a Recombinant Form of the Protein in Mammalian Cells A Novel Plasmid-Bascd System for studying Gene Rearrangements in Mammal|art Cells Tissue-Specific Expression of an llbeta-Hydroxysteroid Dehydrogenase with a Truncated N-Terminal Domain . a Potential Mechanism for Differential intracellular Localization Within Mineralocortienid Target Cells Cloning of a H.man. Alpha(1,3)-Focosyltransferase Gene that Encodes ELFT But Does not Confer ELAM-1 Recognition on Chinese Hamster Ovary Ceil Transfectants Saeeharomyces Cerevistae Cells Secreting an Aspergtllus Niger Bcta-Galactostdase Grow on Whey Permeate. Btotechnology High-Level Expression of Functional Platelct-Activatiog Factor Receptors on a H,man B-Lymphoblastoid Cell Line Conversion of • the Lac-eepressor into an AIiosterically Resulated Transcriptional Activator for Mpmmaltan CellS Functional Expression in Mammalian Cells of a Full-Leatgth CDNA Coding for the Pp42/MAP Kinase (p42mapk) Protein HOmcosis in the o• Mouse Induced by a Null Mutation in the Hox-~l Gene Efficient Gene Expression in Mammal|a~l Cells from a Dicistronic Transcriptional Unit in an Improved Rctrovlral Vector A New Generation of Animal Cell Expression Vectors Based on the Sel Forest Virus Replicon Cloning and Functional Expression of a Vascular Smooth Muscle Endothelin-1 Receptor Raccoon Poxvirus Recombinants Expressing the Rabies Virus Nucleoprotein Protect Mice Against Lethal Rabies Virus Infection Expression of a Foreign Gene in Cats Reconstituted with Retrovival Vector Infected Autologous Bone Marrow A System for Study of Coronavirus Messenger RNA Synthesis -a Regulated Expressed Subgenomic Defective interfering RNA Results from Intergenic Site Insertion. y V~rol Endocytusis and Targeting • • of Exogenous HIV-1 Tat Protein. EMBOJ1991 Design, Construction and Function of a Multicopy Display Vector Using Fusions to the Major Coat Protein of Bacteriophage-M13 Cloning and Sequence Analysis of Brain CDNA Encoding a Xcnopus-D2 Dopamine Receptor Cloning and Expression of Hepatitis. B Surface Antigen in the Yeast Kluyvcromyces-Lactis Cloning of the Drosophila-prospero Gene and Its Expression in Ganglion Mother Cells Development of a Recombinant Baculovirus Expressing an Insect-Selective Neurotoxin -Potential for The Expression and Characterization of Rat IgE Produced by Construction of the Epsilon-Heavy Chain Gent from Exon Modules Inactivation of a Sperm Motility Cenc by Insertion of an Epidermal Growth Factor Receptor Transgene Whose Product is Overexpressed and Compartmentalized During Spermatogenesis Development of a Highly Efftcleut Expression CDNA Cloning System -Application to Oncogene isolation Ovcrexpression of Seleno-Glutathione Pcroxidase by Gene Transfer Enhances the Resistance of T47D Human Breast Cells to Clastogenic Oxidants Structural and Biological Properties of Human Recombinant Myeloperoxidasc Produced by Chinese Hamster Ovary Cell Lines Analysis of N-Mcthyl-N-Nitrosourea-Induced Mutations in a Shuttle Vector plasmtd Propagated in Mouse O6-Mcthylguanine-DNA Methyltransferase-Defieient Cells in Comparison with Proficient Cells Expression of Insulin-Like Growth Factor-IA and Factor-EB Messenger RNA ha Ht,fn~n Liver, Hepatoma Cells, Macrophage-Like Celis and Fibroblasts Overproduction of Adenovirus DNA Polymerase and Prcterminal ProteIn in HeLa Cells LCR/MEIa a Versatile System for High-level Expression of Hcterologous Proteins tn Fa-ythrnid Cells Intramolecuiar Relationships in Cholinesterases Revealed by Oocyte Expression of Site-directed and Natural Variants of Human BCIIE Molecular Cloning of CDNA and a Chromosomal Gene Encoding GPEI-BP, a Nuclear Protein Which Binds to Granulocyte Colony-Stimulating Factor Promoter Element-1 Binary System • . for Regulating Transgene Expression in Mice: Targeting lnt-2 Gene Expression with Yeast GAL4/UAS Control Elements Adeno-Associated Virus Rep Proteins Produced in Insect and Mammalian Expression Systems -wild-Type and Dominant-Negative Mutant Proteins Bind to the Viral Replication Origin Production of Analytical Quantities of Recombinant Proteins in Chinese Hamster Ovary Cells Using Sodium liutyrate to Elevate Expression Cloning of Muscle • • isoforms of Neuronal Cell Adhesion Molecule Using an Episomal Shuttle Vector The Expression of Murinc Hox-2 Genes is Dependent on the Differentiation Pathway and Dispiays a Collinear Sensitivity to Rettnoic Acid in F9 ceils and Xenopus Embryos Cloning of the LOWaffinity Murinc Granulocyte-maerophage Colonystimulating Factor Receptor and Reconstitution of a High-afftnity Receptor Complex The Mechanisms by Which a Human Apolipoprotein-B Gene Enhancer and Reducer Interact with the Promoter are Different in Cultured Cc]~ of Hepatic and Intestinal Origin Similarities and Differences in the Function of Regulatory Elements at the 5' End of the Hnrnan Apolipoprotein-B Gene in Cultured Hepatoma (HepG2) and Colon Carcinoma (CaCo-2) Cells Expression of the Wilms Tumor Gene WT1 in the Murine Urogenital System Receptor Function in Mice Bearing Anttseuse RNA Ttmmgene Molecular Cloning and Characterization of the Mouse UDP-N-Acctylglucosamine -Alpha-3-D-matmoside Beta-1,2-N-Acetylglucosamtnyltransferase-I Gene Gene Tratlsfer into Human Lymphocytes by a Defective Human Vmm,modeflciency Virus Typed Vector. f Virol Reirovirus Promoter-Trap Vector to Induce LacZ-Gcne Fusions in M~mm~li~ft Cells Expression of Human Glycophorin-A in wild TFpe and Glycosylation-Deficieut Chinese Hamster Ovary Cells -Role of Normal-Linked and Ortho-Linked Glycosylation in Cell Surface Expression Complete Sequence of the Allergen Amb-alI -Recombinant Expression and Reactivity with T-Cells from Ragweed Allergic Patients Adenovirus-Mediated Transfer of a Recombinant Alphal-Antitrypsin Gene to the Long Epithelium lnvivo Endothelin-1 is Expressed and Released by a Human Endothelial Hybrid Cell Line (EA Calling Parvovirus Empty Capsids Produced by Expression in a Bacnlovirus Vector -use in Analysis of Viral Properties and Immunization of DOgs Complete Hepatic Regeneration after Somatic Deletion of an Albumin-plasminogen Activator Transgene Long-Term Invivo Expression of Rctrovirus-Mediated Gent Transfer ill Mouse Fibroblast Implants Development and Function of T Cells in Mice Rendered Interleukin-2 Deficient by Gene Targeting Retrovirtm-Mcdiated Gene Transduction into Long-Term Repopulating Marrow Cells of Dogs A Dominant Positive and Negative Selectable Gene for use in Mammr.lian cells Cell-type-specific and Hypoxia-indueible Expression of the Hnm~n Erythropoietin Gene in Transgcnic Mice • Multiple Tissue-specific Elements Control the Apolipoprotcin E/C-I Gene LoCUS in Trarmgenic Mice Stable Expression of the Mouse Nicotinic Acctylcholine Receptor in Mouse Ftbroblasts -Comparison of Receptors in Native and Transfected Cells Long Term Invivo Expression of Genes Introduced by Retrovirus Mediated Transfer into Mammary Epithelial Celis. f Virol Promoter Interactions in Retrovtrus Vectors Introduced into Fibroblasts and Embryonic Stem Cells • Targeted Disruption of the Gsrc Proto-oncogene Leads to Osteopctro6is in Mice TwO Viral Polypeptides Encoded by the E1 and E2 Open Reading Frsmes. EMBOJ1991 I4-tsh Level Expression of Enzymatically Active Mature Human G~mrn~-giutamyltransferase in Trausgenic V79 Chinese Hamster Cells Identification of Transcription Factors Required for the Expression of M~mm~ltu*l U6 Genes in Vitro Carcinoma Induction Following Direct Inaltu Transfer of V-Ha-Ras into Rat M~mm~ry Epithelial Cells Using Replication-Defective Retrovfrus Vectors Epstein-Barr Virus (EBV) Recombinants -use of Positive Selection Markers to Rescue Mutants in EBV-Negative B-Lymphoma Cells Resistance to Human lmm,modefictency Virus Type-I (HIV-1) Infection in Human CD4+ Lymphocyte-Derived Cell Lines Conferred by Using Rctroviral Vectors Expressing an HIV-1 RNA-Specific Ribozyme A • 3 Kb Sequence from the Mouse Cellular Retinoicacid-binding Protein Gene Upstream Region Mediates Spatial and Temporal LacZ Expression in Transgenlc Mouse Embryos Regulation of Gene Expression by Insulin and Tumor Necrosis Factor-alpha in 3T3-L1 Cells -Modulation of the Transcription of Genes Encoding ACYI-CoA Synthetase and Stearoyl-CoA Desatorase-1 Cloning of a Putative High.affitflty Kahtate Receptor Expressed Predominantly in Hippocampal CA3 Ceils Construction of Vaccinia Virus Recombinants Expressing Several Measles Virus Proteins and Analysis of Their Efficacy in Vaccination of Mice Immortalization of Normal Human Bronchial Epithelial Cells by Human PaptlloJ3~viruses 16 or 18 HARMS CC: v~rtalizmion of Normal Human Bronchial Epithelial Cells by Human Paplllomaviruses 16 or 18 Regulation of the Expression of a Secreted Acidic Protein Rich in Cysteine (SPARC) in Human Fibroblasts by Transforming Growth Factor-beta -Comparison of Transcriptional and Post-TranseciptionaI Control with Fibronectin and Type-I Collagen High Level Expression of Active Human AIpha-l-antitrypsin in the Milk of Transgedic Sheep Locating a Low Density Lipoprotein-Targeting Domain of Human Apollpoproteln-B-100 by Expressing a Mintgene Construct in Transgenic Mice Analysis of Point Mutations in an Ultraviolet-Irradiated shuttle Vector Plasmid Propagated in Cells from Japanese Xeroderma Pigmentosum Patients in Complementation GrOUp-A and Group-F. CancerRes Expression of Catalase and Myeloperoxid~se Genes in Hydrogen Peroxide-Resistant HI~60 Cells • Activation of BPV-1 Replication In Vitro by the Transcription Factor E2 Expression of the Cystic Fibrosis Transmembrane Conductance Regulator Gene in Cells of Non-epithelial Origin Purification and Characterization of a Human Recombinant T-Cell Protein-Tyrosine-Phosphatase from a Baculovirus Expression System A Single Upstream Glucocorticoid Response Element Juxtaposed to an AP1/ATF/CRE-Ltke Stte Renders the Chicken Glutamine Synthetase Gene Hormonally Inducible in Transfected Retina Selective Transcription of Rat Proenkephalin Fusion Genes from the Spermatogenic Cell-Specific Promoter in Testis of Transgerdc Mice lnternal • . ~tion of Translation in Retroviral Vectors Carrying Plcornavir~ 5'Nontranalated Regions The Sequence Context of the • ~tion Codon in the Encephalomyocarditts Virus Leader Modulates Efficiency of internal Translation Initiation The Encephalomyo-• * carditis Virus internal Ribosome Entry Site Allows Efficient Coexpressinn of Two Genes from a Recombinant Provtrus in Cultured Cells and in Embryos Improved Vectors for Stable Expression of Foreign Genes in Mnmmal|a~ Cells by use of the Untranslated Leader Sequence from EMC Virus Coustrnction • of a Blfonctlonal MRNA in the Mouse by Using the internal Ribosomal Entry Site of the Encephalomyocardhis Virus A Spleen Necrosis Virusbased Retroviral Vector Which Expresses Two Genes from a Dicistronic MRNA • • Cardioviral Internal Ribosomal Entry Site is Functional in a Genetically Engineered Dicistronic Pollovirus W~: R~trovLt~ Vectors Collt~tntng Putative Internal Ribosome Entry Sites: Development of a Polycistronic Gen¢ Transfer System and Applications to Hnm~t Gene Therapy An internal Ribosome Bindin~ Site can be used to Select for Homologous Recombinants at / an Immunogiobulin Heavy-chain Locus DNA viral vectors Related reviews: Vaccinia and other poxvirus expression vectors Adeno-associated virus vectors Regafated • Expression of Foreign Genes in Vaccinla Virus Under the Control of Bacteriophage 1-/RNA Polymerase and The E. Colt Lac Repressor A Pair of SelectabIc Herlk~mtrus Vectors for Simultaneous Gta3e Expression in Human Lymphoid Cells Biologically Active and Amidated Cecropin Produced in a Bacuiovir~ l~resslon System from a Fusion Construct Containing the ANONYMOUS: Gene Transfer by Adenvoirus Coinfection with Recombinant Vaccinia Viruses Expressing Pollovirus-Pl and Poliovtrus-P3 Proteins Results in Polyprotein Processing and Formation of Empty Capsid Structures Mutational Analysis of the • Core, Spacer and Initiator Regions of VaccinLa Virus intermediate Class Promters Cellular Expression of a Functional Nodavirus RNA Repllcon from Vaccinia Virus Vectors Expression of the P Protein of the Human Hepatitis B Virus in a vaccinia Virus System and Detection of the Nucleocapsid Associated P gene Product by Radiolabelllng at Newly introduced Phosphorylation Sites Polyhedron Initiator Codon Altered to AUU Yields Unexpected Fusion Protein from a Baculovirus Vector Efficient CDNA Cloning by Direct Phenotypic Correction of a Mutant Human Cell Line (HPRT*) Using an Epstein-Barr Virus Derived CDNA Expression Vextor Secretion of Biologically Active Leech Hirudin from Bacnlovtrns-Infected Insect Ceils Recombinant Poxviruses Extracellular Vaccinia Virus • Formation and Cell-to-cell Virus Transmission are Prevented by Deletion of the Gene Encoding the 37,000 Dalton Outer Envelope Protein largescale Eradication of Rabies Using Recombinant Vaccinia-Rabies Vaccine Immunisation with Canarypox Virus Expressing Rabies Glycoproteln cloning and Expression of a Chick Liver Ghitathione S-Transferee CL 3 Sulomit with the use of a Baculovirns Expression System Effect of lmtron a from Human Cytomegalovirus (towne) Immediate-early Gene on HeteroloRous Expression in M~mmalt~ Prevention of Apoptosis by a Baculovtrus Gene During Infection of Insect Cells Safety of and lmmtmOlogical Response to a Recombinant Vacelnla Virus Vaccine Expressing HIV Envelope Glycoproteim Lancet Catalytic Subunit of Human DNA Polymerase-alpha Overproduced from Baculovtrus-Infected Insect Cells -Structural and Enzymological characterization The Sendal Virus P Gene Expresses Both an Essential Protein and an Inhibitor of RNA Synthesis by Sh~ Modules Via MRNA Editing Protection Against Lethal Cytomegalovirus Infection by a Recombinant Vaccine Containing a Single Nonameric T-Cell Epitope Recombinant Bacolovir~es Expressing Yellow Fever Virus-E and NS1 Proteins Elicit Protective Immunity in Mice. f Gen Viro11991 Systemic Expression of a Bacterial Gene by a Tobacco Mosaic Virus Based Vector Preparation of Cell • • Cultures and Vaccinia Virus Stocks Biological and Immunological Properties of Human Immunodeflciency Virus Typed Envelope Glycoprotctn -Analysis of Proteins with Truncations and Deletions Expressed by Recombinant Vaccinin Viruses Correction of Defective PKA Regulation of Outwardly Rectifying Chloride Channels by Insertion of Cystic Fibrosis Transmemebrane Regulator into CF Airway Epithelial Cells Protective Immunization Against Epstein-Barr Virus-induced Disease in Cottontop T~m~eins Using the Virus Envelope Glycoprotein Gp340 Produced from a Bovine Paplllomavir~ Expression Vector Expression invivo and Imritro of Foreign Genes Directed by A-Type inclusion Body Hybrid Promoters in Recombinant Vacctltia Viruses. f Hrol Baculovirus-Expressed Glycoprotein-H of Herpes SImplex Virus Typed (HSV-1) Induces Neutralizing Antibody and DeLayed Type Hype~etm~ty Responses, But Does not Protect Immunized Mice Against Lethal HSV-1 Challenge Expression of Herpes Simplex Virus Type-1 Glycoprotein-I in Bacuiovtrus -Preiiminary Biochemical Characterization and Protection Studies A SImplified Method for the Production of Recombinant Bacuiovirus Expression and Purification of Functional G-Protein Alpha-Subunits Using a Baculovtrus Expression System Bacnlovirus-Mediated Expression of Hllman Apolipoprotein-E in ManducwSexta Larvae Generates Particles that Bind to the Low Density Lipoprotein Receptor Generation of Recombinant Bacnlovirns Via Liposome-Medlated Tran~fection Expression and Post-"13ransLational Processing of Preproceccopin-A Using a Bacuiovirus Vector A Novel Strategy for Converting Recombinant Viral Protein into High Immaunogeulc Antigen A New Strategy to Improve a Cauliflower Mosaic Virus Vector In Vitro T~ta*iption of Bacuiovirus Immediate Early Genes: Accurate MRNA Initiation by Nuclear Extracts from Both Insect and Hnman Cells Protection of Macaques Against SIV Infection by Subunit Vaccines of SIV Envelope Glycoprotcin Gpl60 Baculovtrus-Medlated Expression and Characterisation of Rat Glycogen Synthase Kinase-3beta, the MAmmnliar~ Homologue of the Drosophila-mclanogaster-zeste-white3sgg Expression of Functional Bunyamwera Virus-L Protein by Recombinant Vaccinia Viruses A Recombinant Vaecinia Virus ExpressIng Hepatitis-A Virus Structnral Polypeptides -Characterization and Demonstration of Protective Immunogeulcity. f Gen Viro11991 Assessment of Virus Production and Chinrampherflcol Acctyl Transferese Expression by Insect Ceils in Serum-Free and Serum-Supplemented Media Using a Temperature-Sensitive Baculovirus Expression of a Pheromone-Binding Protein in Insect Cells Using a Bacuioviras Vector Conserved Chromatin Structure in • c-myc 5"-Flanking DNA After Viral Transduction Defective and Nondefecttve Adenovirus Vectors for Expressing Foreign Genes invitro and invivo Expression of Bovine Herpesadrus-1 Glyeoprotein Giv by Recombinant Baculovirus and Analysis of Its Immunogenic Properties Raccoon Poxvirus Recombinant° Expressing the Rabies Virus Nucleoprotein Protect Mice Against Lethal Rabies Virus InfectimL J Viro11991 A Rapid Method for Co°mid Cloning Baculovirus-Mcdiated Expression of the Human Vitamin-D Receptor -Functional characterization, Vttamln-D Response Element interactions, and Evidence for a Receptor A,=tltafy Factor Transcriptional Repression Mediated by the WTI Wllms Tumor Gene Product Expression of Foreign Epitop¢s on Recombinant Occlusion Bodies of Baculoviruses introduction of Foreign DNA • • into the Vaccinta Virus Genome by In Vitro Ligation: Recombination-independent Seiectable Cloning Vectors Mapping of Deletions in the Genome of the Highly Attenuated Vacclula Virus MVA and Their Influence on Virulence The use of African Cassava Mosaic Virus as a Vector System for Plants Characterization and Purification of the Biologically Active V-vis Protein Generated in Silkworm Larvae with the Bombyx Mori Nuclear Polyhedrosis Virus Vector System PrOteCtiOn Against Marek's Disease by a Fowlpox Virus Recombinant Expressing the Glycoprotetn-B of Marek's Disease Virus Expression of the A-Antigen (gp57-65) of Marek's Disease Virus by a Recombinant Baculovtrus PANG y, FRUTOS R, FEDEPdCI BA: Synthesis and Toxicity of Full-Length and Truncated Bacterial CrylVD Mosqultoeidal Proteins Expressed in Lepidoptexan Cells Using a Baculovirus Vector Deletion of Sequences Utmtream of the Proteinase Improves the Proteolytic Processing of Human Immunodeficiency Virus Type-1. Proc Natl Acad Sct U S A 1991, 88:4776~780. pETERSON C, LEGERSKI R: High-Frequency Transformation of Human Repair-Deficient Cell IMtes by an Epatein-Barr Virus-Based CDNA Expression Vector Overproduction of Rat 1,25-Dthydroxyvltamtn-D3 Receptor in Insect Cells Using the Baculovirus Expression System Strn~xwal Proteins of Hog Cholera Virus Expressed by Vacclula Virus -Further Characterization and Induction of Protective Immunity Canine Parvovirus Empty Capsids Produced by Expression in a Baculovirus Vector -use in Analysis of Viral Properties and lmmxlnization of Dogs Targeted Integration of Adeno-assocLated Virus (AAV) into Human Chromosome 19. EMBOJ1991 Constrnction of • chimeric Vacclnia Viruses by Molccadar Cloning and Packaging STOW ND: Herpes Simplex Vires Type-1 Origin-Dependent DNA Replication in Insect Cells Using Recombinant Baculoviruses Baculovirus Recombinant Expressing a Secreted Form of a Transmembrane Carcinoma-Associated Antigen Non-replicating, Safety-tested • • VaccinLa Virus Vector Efficiently Expresses Recombinant Genes NYVAC: a Highly Attenuated Strain of Vacclnta Virus Vaecin]a Virus Recombinant° Expressing Either the Measles Virus Fusion or Hema~Iutinin Glycoprotein Protect Dogs Against Canine Distemper Virus Challenge Efficacy suidies on a Can~-Rabies Recombinant Virus Neutralizing Antibodies Induced by Recombinant Vacciula Virus Expressing Varicella-Zoster Vfrus-gpIV Enhanccmgnt of the Vaccinia Virus/Phage-T7 RNA Polymerase Expression System Using Encephalomyocardltis Virus 5'-UntransLated Region Sequences Regulated High Level Expression of a Human Gamma-globin Gene introduced into Erythroid Cells by a Novel Adenc~ussoeiated Virus Vector Purification and Initial Characterization of the Lymphocyte-Specific Protein-Tyxosyl Klnase P561ck from a Baculoviras Expression System A Bacuioviras Dual Ex~pression Vector Derived from the Autographa-Caltiorulca Nuclear Polyhedrosis Virus Polyhedrin and Pl0 Promoters -Co-Expression of Two Influenza Virus Genes in Insect Cells Bactdovtras Defective interfering Mdes are Responsible for Variations in Recombinant Protein Production as a Function of Multiplicity of Infection ~rtallzatton of Normal Human Bronchial Epithelial Cells by Human Paplllomaviruses 16 or 18 The Hepatitis-A Virus Polyprotein Expressed by a Recombinant Vaccinla Virus Undergoes Proteolytic Processing and Assembly into Vtruslike Particles Baculovirus Expression of the Human Paplllomavirus Type-16 Capsid Proteins -Detection of L1-L2 Protein Complexes Recombinant Fowlpox Viruses Expressing the Glycoprotein-B Homolog and the Pp38 Gene of Marek's Disease Virus Purification and Characterization of a Human Recombinant T-Cell Protein-Tyrosine-Phosphatase from a Baeu]ovim~ Expression System The Hygromycin-Resist ance-Encoding Gene as a Selection Marker for Vac~ Virus Recombinant• VaccLnla Virus-Mediated Expression and Identification of the Human Poliovirus Receptor Internal Intt~Aon of Translation in Retroviral Vectors Carrying Picornavle~-5' Nontranslated Regions A Novel Approach for Establishing Common or Random Integration Loci for Retroviral Genuine• Immune Response and Resistance to Rous Sarcoma Virus Challenge of Chickens Immunized with Cell-Asmx~lated Glycoproteins Provided with a Recombinant Avian Leukosis Virus Expression of Human lmmtmodeficiency Vtrns Type-1 (HIV Proteins from Chimeric HIV-X-poliovirus Mtnireplleons Long-term Improvement of Hyperchol~terolemla after Ex Vlvo Gene Therapy in LDLR-deticient Rabbits Retroviral Expression of Recombinant P47(phox) Protein by Epstein-Barr Virus-Transformed Lymphocytes-B from a Patient with Autosomal Chronic Gt~lnulolnatons Disease Improvement of Avian Leukosis Virus (ALV)-based Retrovirus Vectors by Using Different Cis-acttng Sequences from ALVs A Rapid in V/tro Assay for HIV DNA Integration In Vivo Gene Transfer with Retrovtral Vector-producer Cells for Treatment of Experimental Brain Tumors • • Gene Therapy Via Primary Myoblasts: Longterm Expression of Factor IX ]Protein Following TranspLantation In Vivo Attenuating Mutations in the E2 Glycoprotein Gene of Venezuelan Equine Encephalitis: Construction of Single and Multiple Mutants in a Full-Length Clone Construction of a Replication*competent Murtne Rctrovirns Vector Expressing the Human Immmtodeficiency Virus Type-1 Tat Transactivator Protein High Efficiency Formation of • Influenza Virus Transfectants An Influenza • • Virus Containing Nine Different RNA Segments Protective Immunization Against El~stein-Berr Virus-Induced Disease in Cottontop Tamarins Using the Virus Envelope Glycoprotein Gp340 Produced from a Bovine Papillomavirus Expression Vector Complementatlon Between Sindbis Viral RNAs Produces Infectious Particles with a Bipartite Genuine Infectious *• Sindbis Virus Transient Expression Vectors for Studyin s Antigen Processing and Presentation Repair and Mutagenesis of the Genome of a Deletion Mutant of the Coronavtrus Mouse Hepatitis Virus by Targeted RNA Recombination Efficient Gene Expression in Mammglto¢ Cells from a Dicistronic Transcriptional Unit in an Improved Rctroviral Vector *• Influenza a Virus Transfectants with Chimeric H~t~lntinins Containing Epitopes from Different Subtypes A New Generation of ~jn|mal * • Cell Expression Vectors Based on the SernBk! Forest Virus Replicon /n Vitro • Mutagenesis of a M-length CDNA Clone of Semlild Forest Virus: the Small 6,000-Molecularweight Membrane Protein Modulates Virus Release • Infectious Enveloped RNA Virus Antigenic Chimeras LYNCH CM, MILLER AD: Production of High-Tlter Helper Virus-Free Retroviral Vectors by Cocultivation of Packaging Cells with Different Host Ranges Construction and Properties of Retrov/rus Packaging Cells Based on Gibbon Ape Leukemia Virus. f Virol Stromal Support Enhances Cell-free Retroviral Vector Transduction of Human Bone Marrow Ions-term Culture-initiating CeiLs Retrovtral Vectol~ Containing Putative Internal Ribosome Entry Sites: Development of a Polycistronic Gene Transfer System and Applications to Human Gene TheraI Complementation Between Avirulent Newcastle Disease Virus and a Fusion Protein Gene Expressed from a Retrovirus Vector -Requirements for Membrane Fusion An Influenza a Virus Containing Influenza B Virus 5'and 3"Noncoding Regions on the Neuraminidase Gene is Attenuated in Mice Unusually High Frequency of ReconstRutinn of Long Terminal Repeats in U3-Minus Rctrovirus Vectors by DNA Recombination or Gene Conversion *• Genetically Modified Skin Flbroblasts Persist Long after Transplantation by Gradually inactivathxg Introduced Genes Rescue of a • Foreign Gene by Sendai Virus • Infectious Defective Interfering Particles of VSV from Transcripts of a CDNA Clone Replication-Competent Retrovirus Vectors for the Transfer and Expression of Gene Cassettes in Avian Cells The Efficient Intracellular Sequestration of the Insulin-regulatable Glucose Transporter (GLUT-4) is Conferred by the N Terminus Gene Transfer into Human Lymphocytes by a Defective Human Immunodeficiency Virus Type-1 Vector Rctrovirus Promoter-Trap Vector to Induce LacZ-Gene Fusions in M~mmaliaq Cells Long-term In • • Vivo Expression of Retrovir~-medlated Gene Transfer in Mouse Fibroblast Implants Cloning of an NF-IcB Subunit Which Stimulates HIV Transcription in Synergy with Retrovirus-Mediated Gene Transduetion into Long-Term Repopulating Marrow Cells of Dogs Long Term Invivo Expression of Genes Introduced by Rctrovirus Mediated Transfer into M~mmury Epithelial Cells Promoter interactions in Rctrovirus Voctors Introduced into Fibroblasts and Embryonic Stem Cells Site-Specific Stable Insertion into the Human Cytomegalovirus Genuine of a Foreign Gene Under Control of the SV40 Promoter Carcinoma Induction Foliowhlg Direct Insito Transfer of V-Ha-Ras into Rat M~mmat~}r Epithelial Cells Using Replication-Defe~ive Rctrovirus Vectors lS Type-1 (HIV-X) Infection in Human CD4+ Lymphocyte-Derived Cell Lines Conferred by Using Retroviral Vectors Expressins an HIV-1 RNA-Spocific Rtbozyme. f Vtro11991 Post-translational modifications Related review: Protein glycosylation High-Level Secretion of Correctly Processed Recombinant H.man Lnterleuktn-1-beta in Kluyveromyces-Lactis POSt-Transcriptional Regulation in Higher Eukaryotes -the Bole of the Reporter Gene in Controlling Expression Selective Prnteulytle Cleavage of Recombinant Bnmao Evidence for a Critical Role of the C-Terminus The Full Precursor of the KDa Oxygen-Evolvio4~ Complex Protein of Wheat is Exported by Escherichta-coli and Processed to the Mature SiZe Truncation of the Extended Carboxyl-Terminal Domain Increases the Expression and Regulatory Activity of the Avian Beta-Adrenergic Receptor Expression of Human Glycophorin-A in Wild Type and Glycosylation-Deficient Chinese Hamster Ovary Cells -Role of Normal-Hnked and Ortho-Linked Glycosyiation in Cell Surface Expression Structures and Flulctional Roles • • of the Sugar Chains of Human Erythropoiettns Phax'lnacokioetics in Chltnpanzees of Recombinant Human Tissue-Type PLasminogen Activator Produeed in Mouse C127 and chinese Hamster Ovary Ceils The Hepatitis-A Virus Polyprotoin Expressed by a Recombinant Vaecioia Virus Undergoes Proteolytie processing and Assembly into Viruslike Particles A New Signal Peptide Useful for Secretion of Hcterologous Proteins from Yeast and Its Application for Synthesis of Htfudin A 30-Residue-Long Export Initiation Domain Adjacent to the Signal Sequence is Critical for Protein Translocation Across the Inner Membrane of Escherichia-CoiL Proc Natl Acad Sc~ The Primary Pathway of Protein Export in E-CoIL Cell Production Processes of Recombinant IDlbcta from Bacillus Sub•ills: Comparison Between Intracellular and Exocellular Expression Secretion of Biologically Active Leech Hirudin from Baculoviras-Infected Insect Ceils *• PC1 and PC2 are Proprotein Convertasas Capable of Cleaving Proopiomelanocortin at Dtstiuct Pairs of Basic Residues Production of Mouse Epidermal Growth Factor in Yeast -High-Leval Secretion Using Plehia-Pastoris Strains Containing Multiple Gene Copies Mutations of the Alpha-Galactosidase Signal Peptide Which Greatly l~nhance Secretion of Hcterologous Proteins by Yeast Saccharomyees Cerevisiae Secreting an Aspergiilus Niger Beta-GaLactosidase Grow on Whey Pecmeate Cloning and Expression in Eseherfehia-coH of the Serratia-Marcescens Mctalloprotease Gene -Secretion of the Protease from E-COB in the Presence of the Erwinta-Chrysanthemi Protease Secretion Functions The Full Prectlrsor of the KDa Oxygeu-Evolving Complex Protein of Wheat is Exported by Escheriehia-Coli and Processed to the Mature Size Identification of a Geue Fragmeut Which Codes for the 364 Amino Acid Residues of a SecA Homologue from Bactllus-subtlBs -Further Evidence for the Conservation of the Protein Export Apparatus in Gram-Positive and Gram-Negative Bacteria Immunolocaltzation • of Kex2 Protease Identifies a Putative Late Golgi Compartment Ln the Yeast Sacchromyces Cerevtsiae Regulation ** of PACE Propeptide Processing Activity: Requirement for a Post Endoplasmic Reticulum Compartment and Autoproteolytic Activation Preferred Sequence • Requirements for Cleavage of Pro-yon Willebfand Factor by Propeptide-Proceasing Enzymes Secretion of Active Truncated CD4 into Escherichia-coli Peripiasm Mofecuiar Dissection of the Secretory Pathway One of Three Transmembrane Stretches is Sufficient for the Functioning of the SecE Protein, a Membrane Component of the E Coil Secretion Machinery Selection of Secretory Protein-Encoding Genes by Fusion with PHO5 in Sarcharomyces-Cerevisiae Bacnloviras Recombinmat Expressing a Secreted Form of a Transmembrane Carcinoma-Assochtted Antigen A Positive Residue in the Hydrophobic Core of the Eseherichis-Coll Hpoprotein Signal Peptide Suppresses the Secretion Defect Caused by an Acidic Amino Terminus TnbiaM -a Transposon for Directly Tagging Bacterial Genes Encoding Cell Envelope and Secreted Proteins Polding Mechanism of Mutant Hnmaa Lysozyme C77/95A with Increased Secretion Efficiency in Yeast Kex2 Like Endoproteases PC2 and PC3 Accurately Cleave a Model Prohormone in Mammnlinn Cells: Evidence for a Common Core of Neuroendocrine Processing Enzymes Structure of Yeast PGKL 128-kDa Klller-Toxio Secretion Signal Sequence -Processing of the 128-kDa Killer-Toxin-Secretion-Signal -Alpha-Amylase Fusion Protein Muhicopy Suppression -an Approach to Understanding Intracellalar Functioning of the Protein Export System Effects of Replacement of Promoters and Modification of the Leader Peptide Region of the Amy Gene of Steeptomyces-Griseus on Synthesis and Secretion of Alpha-Amylase by Streptomyces-Lividans Secretion of Biologically Active Leech Hirudin from Baculovirus-Infected Insect Cells Baculovtrus Gp64 Cene Expression -Analysis of Sequences Modulating Early Transcription and Transactivation by lEX Prevention of Apoptosts by a Baculovirus Gene Dr•ring Inaction of Insect cells Catalytic Subtmit of Human DNA Polymerase-alpha Overproduced from Baculovirus-Infected Insect Cells -Structural and Enzymological Characterization Catulyzed Trimming of High-Matmose Glycans in Nouln~cted and Baculovirus-Infected spodoptera-Frugiperda Cells OEPLB-SF-21AE) -a Possible Contributing Regulatory Mechanism for Assembly of Complex-Type Oligosaccharides in Infected cells Expression of Porcine Complement Cytolysis Inhibitor Messenger RNA in Cultured Aortic Smooth Muscle Cells -Changes During Differentiation Invitro Functional Expression of the Human Growth Factor Activatable Na+/H+ Antiporter (NIEE-1) in Baculovinls-Iufected Cells Expression of the Saccharomyces-Cerevisiae Kex2p Endoprotease in Insect Cells -Evidence for a Carboxy-Termlnal Autoprocessing EvenL EurJBiochem Expression of Biologically Active Human Corticosteroid Binding Globulin by Insect Cells -Acq~daltinn of Function Requires Glycosyiation and Transport Targctted Gene Replacement in Drosophila Via P Element-iodueed Gap Repair Site-Specific Recombination Between Homologous Chroxuosomcs in Drosophila Baculovirus-Mediated Expression of Hnman Apollpoproteln-E in Manduea-Sexta Larvae Generates Particles that Bind to the Low Density Lipoprotoin Receptor ~x~tession of at• Elfllancer-Binding Protein in Insect Cells Transfected with the Autographa-Calffornica Nuclear Polyhedrosis Viras-Iel Gene Accurate MRNA Initiation by Nuclear Extracts from Both Insect and Human Cells Production and Purification of Recombinant Human Lqterleuldn-5 from Yeast and Bacnlovfrus Expression Systems An Indicator Gene for Detection of C-~mltne Retrotransposition in Trat~geuic Drosophila Demonstrates RNA-mediated Transposition of the IANE I Element Secretion of Single-Chain Urokinase-Type Plasminogen Activator from Insect Cells Baculovirus-Directed High Level Expression of the Hepatitis-Delta Antigen in spodoptera-Frugiperda Cells. f Gen Viro11991 Expression of a Pheromone-Binding Protein in Insect Cells Using a Bacuiovirus Vector Quantification of Cell Culture Factors Affecting Recombinant Protein Yields in Bacniovirus-Infeeted Insect Ceils Purification and Characterization of Human Immunodeflciency Vlrus Typed Nef Gene Product Expressed by a Recombinant Bacnlovirus Developmeni of a Recombinant Bacuiovirus Expressing an Lnsect-Selective Neurotoxtn -Potential for Development of a Recombinant Bacuiovirus Expressing an Insect-seleetlve Neurotoxin: Potential for Pest ControL Biotechnology Chatacteriaation and Purification of the Biologically Active V-vis Protein Generated in Silkworm Larvae with the Bombyx Mori Nuclear Polyhedrosis Virus Vector System Protein Production (bcta-Galactoaidase) from a Baculovirus Vector in spodoptera-Frugiperda and Trichpolusia-Ni Cells in Suspension Culture Improvement of a baculovirus Pesticide by Deletion of the EGT Gene Adeno-Associated Vlrus Rep Proteins Produced in Insect and M~mm~it~x Expression Systems -Wild-Type and Dominant-Negative Mutant Proteins Bind to the Viral Replication Origin Synthesis and Toxicity of Full-Length and Truncated Bacterial CryIVD Mnsqditocidal Proteins Expressed in Lepidopteran Cells Using a Baculovirus Vector Overproduction of Rat 1,25-Dillydroxyvitamin-D3 Receptor in Insect Cells Using the Baculovirus Expression System Expression of the Hnm~n Multidrug Resistance CDNA in Insect Cells Generates a High Activity Drug-Stimulated Membrane ATPase Herpes SImplex Virus Type-1 Origin-Dependent DNA Replication in Insect Cells Using Recombinant Baoflovirases Expression of Rat Liver Glt"~m;-~ Transporters in Xenopns-Laevis Oocytes Enhanced Secretion from Insect Cells of a Foreign Protein Fused to the Honeybee Melittin Signal Peptide Baculovirus Vectors for Multiple Gene Expression and for Occluded Virus Production A Baculovirt~ Dual F2gpression Vector Derived from the Autographa-Californica Nuclear Polyhedrosis Virus PoIyhedrin and Pl0 Promoters -Co-Expression of Two Influenza Virus Genes in Insect Cells Expression of Androgen Receptor in Insect Cells -Purification of the Receptor and Renaturation of Its Steroid-Binding and DNA-Bindhtg Functions Stable Transfornlation of Sorghum-Bicolor Protopissts with Chimeric Neomycin Phosphotransferase-II and Bcta-Glucuronidase Genes The 3'-Terminal Begion of the Hygt~3mycin-B-Resistance Gene is Important for Its Activity in Escherichia-coli and Nicottana-Tabacum Transgenie Plants with Enhanced Resistance to the Fungal Pathogen Rhtzoctonia-Solani Positive and Negative cis-aeting DNA Domains are Required for Spatial and Temporal Regulation of Gene Expression by a Seed Storage Protein Promoter Production of Transgenic Rice (oryza Sativa L) PLants from Ageonomicaily Important Indica and Japonica Varieties Via Electric Discharge Particle Acceleration of Exogenous DNA into Immalllre Zygotic Embryos 8tl'n~xn'es of the Asparagine-289-Linked Oligosacchartdes Assembled on Recombinant H.man Plasminogen Expressed in a Mamestra-Brassicac Ceil Line (IZD-MBO503) The Extensin Signal Peptide Allows Secretion of a Hcterologous Protein from Protoplests Cloning of the Coat Protein Gene from Beet Necrotic Yellow Vein Virus and Its Expression in Sugar Beet Hairy ROOts High Intensity and Blue Light Regulated Expression of chimeric Chalcone Synthase Genes in Transgenic Arabidopsis-Thallana Plants Construction and Characterization of a Yeast Artificial Chromosome Library of Arabidopsis Which is Suitable for Chromosome Walking Entire Maize Chloroplast Genome is Stably Maintained in a A New Strategy to Improve a Cauliflower Mosaic Virus Vector Transcriptional Interference in Transgentc Plants Gemintvirus-Based Shuttle Vectors Capable of Replication in Escheriehia-COli and Monocotyledonous Plant Cells eDNA Cloning and Expression of Brassica-Napns Enoyl-acyl Carrier Protein Reductase in Escherichia-Co1L Plant Mol Bio11991 pEMU -an Improved Promoter for Gene Expression in Cereal Ceils Stable Transformation of Barley Via PEG-Induced Direct DNA Uptake into Protoplasts Construction of Expression Vectors Based on the Rice ACtin-1 (ACt1) 5' Region for use in Monocot Tralxsforml~on The Full Precursor of the KDa Oxygen-Evolving Complex Protein of Wheat is Exported by Eachea'ichia-Coli and Processed to the Mature Size The use of African Cassava Mosaic Virus as a Vector System for Plants Use of Ars18 Based Vectors to Increase Protein Production in Yarrowia Ltpolytica Direct Gene Transfer into Actinidia.Deilc~sa Protoplasts -Analysis of Transient Expression of the CAT Gene Using TLC Autoradiography and a GC-MS-Based Metho~L Plant Mol Bio11991 Agrobacterinm-Rhizogenes LacZ-rolC Gene Expression in Eseherichia-Coli -Detection of the Product in Transgenic Plants Using RolC-Specific Antibodies Binary System for Regulating Transgene Expression in Mice: Targeting/nt-2 Gene Expression with Yeast GAL4/UAS Control Elements Cotransformation and Differential Expression of Introduced Genes into Potato (Solanum-Tuberosum L) Cv Bintje Stress Responses in Alfalfa (Medicago-Sativa L). 11. Molecular Cloning and Expression of Alfalfa Isoilavone Reductase, a Key Enzyme of Isoflavonold Phytoalexln Biosynthesis Modification of the Coding Sequence Enhances Plant Expression of Insect Control Protein Genes SALOHEIMO METAL.: Heteroiogons Production of a Ltgninolytic EnZ~Hle: Expression of the Phlebia Radima Laccase Gene in Tdchoderma RceseL Btotechnology Transient Expression of Beta-Glucuronidase in Arabidopsis-Th~ltaqa Leaves and Roots and Brassica-Napus Stems Using a Pneumatic Particle Gun Synthesis and Secretion of Alpha-Amylase by Rice Callus -Evidence for Differential Gene Expression Custom Pulymerase-Chain-Reaetion Engineering of a Plant Expression Vector In V'xvo Import of a normal or Mutagenized Hcterologons Transfer RNA into the Mitochondria of Transgenic Plants: Towards Novel Ways of Influencing Mitochondrial Gene Expression? Lipofectin -Direct C, ene Transfer to Higher Plants Using Cationic Liposomes Expression of a Monoeot LHCP Promoter in Transgettic Rice TaSghlg Genes for BLast Resistance in Rice Via Linkage to R]FLP Markers Efficient Transformation of Tobacco by Ultrasonication Molecular Cloning and Analysis of Two Tandemly Linked Genes for Pyruvate Klnase of Trypartosoma-Brucel Transformation of a Mcthinnine Auxotrophic Mutant of Mucor-Circlneiloides by Direct Cloning of the Corresponding Wild Type Gene CJonlng and Nucleotide Sequencing of a Novel 7bcta-(4-Carboxybutanamido)cephaiosporanic Acid Acyiase Gene of Baelllus-Laterosporus and Its Expression in Escherichia-Coli and Bacillus-Subtilis Construction of Cloning Vectors for Baeillus-Thuringiensis The Solubility of inclusion Proteins from Bacilius-Thuringtcnsis is Dependent Upon Protoxin Composition and is a Factor in Toxicity to Insects An Induced Messenger RNA Secondary Structure Enhances RcpZ Translation in PLasmid COlIb-Pg Gyrase-dcpendent Stabilization of PSCI01 plasmid Inheritance by Transcriptionally Active Promoters Production Processes of Recombinant IL-lbcta from Bacillus Subttlis: Comparison Between Intracellular and Exoceliular Expression Release of Aeetyleholine byXenopus Oocytes Injected with MRNAs from Cholinergic Neurons Properties of a 72-Ktlodalton Mosquitoctdal ProteIn from Bacillus-Thutingiensis Subsp Morrtsoni PC~14 Expressed in B-Thuringiensis Subsp Kurstald by Using the Shuttle Vector PHT3101 Transformation of Aspe~gillu$ Ot3rzae with a Dominant Selectable Marker Antisense oligonucleotides Inhihlt Intercellular Adhesion Mokcule-1 Expression by Two Distinct Mechanisms Genetic Transformation of Aureobasidinm Puliulans The Sendal Virus P Gene Expresses Both an Essential Protein and an Inhibitor of RNA Synthesis by Sh-ffltog Modules V'm MRNA Editing Xylose (Glucose) Isomerase Gene from the Thermophile Thermus-Thermophllus -Cloning, Sequencing, and Comparison with Other ThermostabIe xylose Isomerases A Versatile Shuttle Cosmid Vector for me in Facherichia-Coli and Actinomycetes A 5'-Terminal Stem-Loop Structure can Stabilize Messenger RNA in F~cherichia-CoiL Genes Dev Dictyostelim-Dtscoldeum as an Expression Host for the Circm~c~porozoite Protein of Plasmodium Codon Choice and Potential Complementarity Between MRNA Downstream of the Initiation Codon and Bases 1471-1480 in 16S Ribosomal RNA Affects Expcesslon of GINS Cloning and Expression in Streptomyces Llvidans of a Chttosanase-Encodiog Gene from the Actinomyccte Kliasatosporia N174 Isolated from SoiL BiotechnolLett Drosophila Acctylcholinesteruse -Expression of a Functional Precursor in Xenopus Oocytes Expression of the Replication Protein Arp of Phasyl Shows Dual Regulation by an Anttsense Promoter The Disulfide Bonds In Antibody Variable Domains -Effects on Stability, Folding Invitro, and Functional Expression in Escherichia-coiL Biochemistry Expression of Human Glucose Transporters in Xenopus Oocytes -Kinetic Characterization and Substrate Spectfielties of the Erythrocyte, Liver, and Brain isoforms A Hummerhead Ribozyme Allows Synthesis of a New Form of the Tetrahymena Rtbozyme Homogenous in Length with a 3" End Blocked for Transesterification Molecular Cloning and Expression of S-(2-aminoethyl)-L-cysteine Resistant Aspartokinase Gene of Corynebacterinm-glutamicutm Production of Pectin Methylesterase from Erwinia-Chrysanthemi B374 in BactIius-Subtilis Highly Bininmtmescent Bacillus-Subtilis Obtained Through High-Level Expression of a LuxAB Fusion Gene Processing, intracellular Transport, and Functional Expression of Endogenous and Exogenous Alpha-beta3 Na, K-ATPase Complexes in Xenopus Oocytes Molecular Cloning and Nuclcotide Sequence of the Glycogen Branching Enzyme Gene (gigB) from BacllIus-Stearothermophilus and Expression in Escherichla-Coli and Bacilius-Subtilis Expression of Cardiac Na Channels with Appropriate Physiological and pharmacological Properties in Xenopus Oocytes The HIe Gene of Streptoalfoteichus-Hindustanus as a New Selectable Marker for Dictyostelinm-Discoideum Confers Resistance to Phleomyc~ Expression and Characterization of an Active and Thermally More Stable Recombinant Antifreeze Polypeptide from Ocean POUt, Macrozoarces Americanus, in Eschcrichia Coil: Improved Expression by the Modification of the Secondary Structure of the MRNA Regulation of Viteliogenin Gene Expression in Transgeuic Caenorhabditts-Elegans -Short Sequences Required for Activation of the Vlt-2 Promoter A System for Study of Coronavirus Messenger RNA Sy~nthesis -a Regulated Expressed Subgenomic Defective Interfering RNA Results from Intergenic Site Insertion Efficient Gene Transfer in C. Elegans: Extrachromosomal Maintenance and Integration of Transforming Sequences Reversible Inbibition of Tomato Fruit Senescence by Antisense RNA Improved L-lystne Production by the Amplification of the Corynbacterium Glutamicum-dapa Gene Encoding Dthydrodiplcolinate Synthetase in E Molecular Cloning and Nueleotide Sequence of the Gene Encoding an Endo-l,4-bcta-glucanase from Bacllius-sp KSM The Expression of Mnrine Hox-2 Genes is Dependent on the Differentiation Pathway and Displays a Collinear Sensitivity to Retinoic Acid in F9 Cells and Xenopus Embryos Use of Alkaline Phosplmtase Fusions to Study Protein Secretion in Bacillus-Subtilts The RepA Rcprcssor can Act as a Transcriptional Activator by Inducing DNA Bends Integron-Bearing Vectors -a Method Suitable for Stable Chromosomal Integration In Highly Restrictive Corynebacteria l~mctional Expression of Transcoabalamtrt-H CDNA in Xenopus-Laevis Oocytes Site-Directed Mntagenesis and Expression of PC2 in Mlcroinjected Xenopus Oocytes TVlJLO is Required for in Vitro Teanscription of the Human U6 Gene by RNA Pulymeruse Hi. ~BO J1991 Prevention of Hnman Imrmmodeficiency Virus Type 1 Integrasc Expression In Escherichta CoB by a Hibozym¢ Construction and Application of Plasmid-Based and Transposon-Based Promoter-Probe Vectors for Streptomyces Spp that Employ a Vibrio-Harveyi Lncifer~se Reporter Cassette Characterization of PGA1, a New Plasmld from Corynebacterinm-Glutamicum LP-6 First Step Toward a Virus-Derlved Vector for Gene Cloning and Expression in Spiroplasmas, Organisms Which Read UGA as a Tryptophan Codon -Synthesis of Chloramphenicul Aeetylteansferase in Sptroplasma Sequences More Than 500 Base Pairs Upstream of the Human U3 Small Nuclear RNA Gene Stimulate the Synthesis of U3 RNA in Erog Ooeytes Constmlction of a Fusion Gene Comprising the Taka-amylasc-A Promoter and the Escherichia-coli Beta-Glucurouidase Gcne and A~alysis of Its Expression in Aspergillus IOUISlg and Expression of a Novel Protease Gene Enc~n~g an Exteaeellular Neutral Protease from Bactllus-Subtilis Characteristics of Bateh Culture of a Recombinant Bacillus-Brevis Excreting a Foreign Estersse Cloning, Characterization, and Multiple Chromosomal Integration of a Bacillus Alkaliee Protease Gene Effects of Replacement of Promoters and Modification of the Leader Peptide Region of the Amy Gene of Streptomyces-Grtsens on Synthesis and Secretion of Alpha-Amylase by Streptomyces-Lividans TtansduL~don of C~Aftain Genes by an Amonomously Replicating Bactlius-Thuringiensis Phage Development of a Novel Bacilins-Subtllts Cloning System Employing Its Neutral Protege ~ Screen Marker