key: cord-0009083-tnaizak6 authors: nan title: Expression systems date: 2002-02-11 journal: Curr Opin Biotechnol DOI: 10.1016/s0958-1669(97)80043-2 sha: 75baf2a54d81046c42b92e8fb45388c52ab048b3 doc_id: 9083 cord_uid: tnaizak6 nan This bibliography is compiled by biotechnologists from the journals listed at the end of this publication. It is based on literature entered into our database between 1st June 1996 and 31st May 1997 (articles are generally added to the database about two and a half months after publication). In addition, the bibliography contains every paper annotated by reviewers; these references were obtained from a variety of bibliographic databases and published between the beginning of the review period and the time of going to press. The bibliography has been grouped into topics that relate to the reviews in this issue. Papers considered by the reviewers to be of special interest • e Papers considered by the reviewers to be of outstanding interest The number in square brackets following a selected paper, e.g. [7] , refers to its number in the annotated references of the corresponding review. Related reviews: Tag games in yeast-the two-hybrid system and beyond (pp 561-568); Gene expression systems in the development of high-throughput screens (pp 629-634)t compared with first-generation vectors in vitro and in rive Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery. Nat Biotechno/199? Generation of recombinant baculoviruses • by direct cloning Expression of ligand-gatad ion channels with the Semliki Forest virus expression system Expression of biologically active mouse and human CD95/APO-1/Fas ligand in the bsculovirus system Rescue of a synthetic chloramphenicol acetyltransferase RNA into influenza virus-like particles obtained from recombinant plasmids Evaluation of tyrosinase minigene co-injection as a marker for genetic manipulation in transgenic mice Efficient transfer of regulated genes in adipocytea and hepatoma cells by the combination of liposomes and replication The GP64 • • envelope fusion protein is an essential beculovirus protein required for cell-to-cell transmission of infection Transovarian transmission of a foreign gene in the silkworm, Bombyx mori, by Autogrspha californica nuclear polyhedrosis virus Genetically engineered poxviruses for • recombinant gene expression, vaccination, and safety Poxviridae: the viruses and their replication. • In Fields Virology Protection against lethal simian immunodeficiency virus SIVsmmPBj14 disease by a recombinant Semliki Forest virus gp160 vaccine and by a 9p120 subunit vaccine Gene transfer and manipulation in the thermophilic cyanobactedum Synechococcus elongatus. Me/Gen Genet Stable expression of mosaic coats of variant surface glycoproteins in Trypanosoma brucei In rive gene delivery and stable transduction of nondividing cells by a lentiviral vector Development of 9ene transfer methods for Rubdvivax gelatinosus $1: Construction, characterization and complementation of a put operon deletion strain Applications of pox virus vectors to • vaccination: an update A helper-dependent adenovirus vector system: removal of helper virus by Cre-madiatad excision of the viral packaging signal Deletion of the myristylation signal allows high-level production of the hepatitis B virus large surface glycoprotain preS1 with vaccinia virus rscombinants Transient expression in insect cells using a recombinant tiaculovirus synthesising bactedophage T7 RNA polymerase High efficiency 5 rain transformation of Escherichia coil Mammalian cell fusion in an electroporation device Comparative • analysis of adenovirus fiber-cell interaction: adenovirus type 2 (Ad2) and Ad9 utilize the same cellular fiber receptor but use different binding strategies for attachmenL J Viro FJsctropermeabilization of intact maize cells induces an oxidative stress A novel expression vector for transfection of bovine MHC class I genes Transient transfection of ecotropic retrovirus receptor permits stable gene transfer into non-rodent cells with murine retroviral vectors Regeneration of transgenic cassava plants (Manihot esculenta Crantz) from microbombarded embryogenic suspension cultures Transfection of mitochondda: Strategy towards a gene therapy of mitochondrial DNA diseases Transformation of Eschedchia coil with large DNA molecules by electroporation Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertional mutagenesis Antigenic and immunogenic analysis of group A and group B respiratory syncytial virus G proteins expressed from recombinant baculoviruses Particle-mediated delivery of recombinant expression vectors to rabbit skin induces high-titered polyclonal antisera (and circumvents purification of a protein immunogen) Transectivation of adenovirus E2-early promoter by EIA and E4 6/7 in the context of viral chromosome Introduction and expression of foreign DNA in isolated spinach chloroplasts by electroporstion HCAR and MCAR: the • • human and mouse cellular receptors for subgroup C adenoviruses and group B coxssckieviruses Immune • • responses to transgene-encoded proteins limit the stability of gene expression after injection of replication-defective adenovirus vectors Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter. Plant Me/BiD/1996 Elongation of the N-glycans of fowl plague virus haemagglutinin expressed in Spodoptera frugiperda (sfg) cells by coexpression of human beta-1,2-N-acetylglucosaminyltransferase I Cationic facial amphiphiles: A promising class of transfection agents • • Adenovirus targeted to heparan*containing receptors increases its gene delivery efficiency to multiple cell types Kovesdi h Targeted adenovirus gene transfer to endothelial and smooth muscle cells by using bispecific antibodies • Identification of recombinant baculovirusea using green fluorescent protein as a selectable marker Vsccinie virus A17L open reading frame encodes an essential component of nascent viral membranes that is required to initiate morphogenesis Cationic lipid binding to DNA: Charactedzation of complex formation Development • of a replication-deficient recombinant vaccinia virus vaccine effective against parainfluenza virus 3 infection in an animal model High efficiency, Iong-tarm clinical expression of cottontail rabbit papillomavirus (CRPV) DNA in rabbit skin following particle-mediated DNA transfer Mechanism of DNA release fTom cationic liposome/DNA complexes used in cell transtaction Enhancement of retrovirus-mediated gene transduction efficiency by transient overexpression of the amphotropic receptor, GLVR-2 Efficient in situ electroporation of mammalian cells grown on microporous membranes Transient subversion of CD4O ligand func'don diminishes immune responses to adenovirus vectors in mouse liver and lung tissues CD40 ligand-dependent T cell • • activation: requirement of BT-CD28 signaling through CD40 DNA gyrase improved DNA transformation of E-coil cells with large recombinant plasmids A hybrid beculovirus-T7 RNA polymerase system for the recovery of an infectious virus from cDNA Amplification, expression, and packaging of a foreign gene by giardiavirus in Giardia lamblia Inducible human immunodeficiency virus type 1 packaging cell lines Characterization and replicase activity of double-layered and single-layered rotavirus-like particles expressed from beculovirus recombinants Gag-Gag interactions in the C-tarminal domain of human immunodeficiency virus type 1 p24 capsid antigen are essential for Gag particle assembly Efficient • membrane targeting of the chick nicotinic ecetylcholine receptor a-subunit in stable insect cell lines Wild-type and insecticide-resistant homo-oligomeric GABA receptors of Drosophila melanogaster stably expressed in a Drosophila cell line Briefing: malaria. Nature --1997 The intrecellular domain of the rabbit prolactin lepidopteran cells Photoaffinity labeling of the human brain cholecystokinin receptor overexpressed in insect cells -Solubilization, deglycosylation and purification Wild type and mutant human heart (R)-3-hydroxybutyrate dehydrogenase expressed in insect cells Coexpression of molecular chaperone BiP improves immunoglobulin solubility and IgG secretion from Trichoplusia ni insect cells Modifying the insect cell N-glycosylation pathway with immediate early baculovirus expression vectors Synthesis of soluble rubella virus spike proteins in two lepidopteran insect cell lines: Large scale production of the E1 protein Influence of agitation and sparging on the growth rate end infection of insect cells in bioreactors and a comparison with hybrtdoma culture Glycosylation is essential for biosynthesis of functional gastric H+,K+-ATPase in insect cells High-level expression in insect cells and purification of secreted monomeric single-chain Fv antibodies Expression of active, secreted human prostate-specific antigen by recombinant baculovirus-infected insect cells on a pilot-scale Characterization of a strawberry gene for auxin-binding protein, and its expression in insect cells Characterization of two recombinant PDE3 (cGMP-lnhibited cyclic nucleotide phosphodiestarase) isoforms, RcGIP1 and HcGIP2, expressed in NIH 3006 murine fibroblasts and Sf9 insect cells Expression of human dopamine ~-hydroxylase in Drosophila Schneider 2 cells Overexpression of neuronal nitdc oxide synthase in insect cells reveals requirement of haem for tetrahydrobioptedn binding Expression and characterization of functionally active recombinant perforin produced in insect cells A modular misexpression screen in Drosophila detecting tissue-specific phenotypes Genetic • • transformation of Drosophila cells in culture by P element-mediated transposition Stable expression of insect GABA receptors in insect cell lines; promoters for efficient expression of Drosophila and mosquito Rdl GABA receptors in stably transformed mosquito cell lines Properties of two insect cell lines useful for the Beculovirus expression system in serum*free culture Grisshammer R: Heterologous expression of G-protein-coupled receptors Co-expression of the alpha subunit of human prolyl 4-hydroxylese with BiP polypeptide in insect cells leeds to the formation of soluble and insoluble complexes -Soluble elpha-subunit-BiP complexes have no prolyl 4-hydroxylese activity Kinetic analysis of alkaline proteese activity, recombinant protein production and metabolites for infected insect (sfg) cells under different DO levels Glycoprotein H of herpes simplex virus type 1 requires glycoprotein I for transport to the surfaces of insect cells Expression in insect cells and purification of a catalytically active recombinant human gastric lipase Stable nuclear transformation of the diatom Phaeodectylum tricornutum Regulation by the ribosome of the GTPase of the signal-recognition particle during protein targeting A two-step • recognition of signal sequences determines the translocation efficiency of proteins Biogenesis of polytopic membrane • proteins: membrane segments assemble within ttenslocation channels prior to membrane integration Relationship between Eschedchia coil growth and deletions of CTG center dot CAG triplet repeats in plasmids ER-associatad and • proteasome-mediated protein degradation: how two topologically restricted events came together Substrata shuttling between the DnaK and GroEL systems indicates a chaperone network promoting protein folding Microsecond protein folding through a compact transition state The translational signal database, TransTerm: Organisms, complete genomes A microsomel ATP-binding protein involved in efficient protein transport into the mammalian endoplasmic reticulum Refolding, isolation and characterization of crystallizable human interferon-alpha 8 expressed in Saccharomyces carevisiae The • cofranslational integration of membrane proteins into the phospholipid bilayer is • multistep process A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae Modmoto Rh The human cytosolic molecular chaperones hsp90, Hsp70 (hsc70) end hdj-1 have distinct roles in recognition of a non-native protein and protein refolding Preference for guanosine at first codon position in highly expressed Escherichie coil genes. A relationship with translational efficiency Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation Molecular chaperones in cellular protein folding ErR degradation of a misfolded luminal protein by the cytosolic ubiquitin-proteasome pathway Structure-based calculation of the equilibdum folding pathway of proteins. Correlation with hydrogen exchange protection factors Coexpression of molecular chaperone BiP improves immunoglobulin solubility and IgG secretion from Trichoplusia ni insect cells High level production of soluble single chain antibodies in smell-scale Eschedchia coil cultures Vieding E: A small heat shock protein stably binds heat-denatured model substrates and can maintain a substrate in a folding-competent state Emergence of preferred structures in a simple model of protein folding Binding of secretory precursor oo polypeptides to a translocon subcomplex is regulated by BiP An upstream activator of transcription coordinately increases the level and epigenetic stability of gene expression Structure of the heat shock protein chaperonin-10 of Mycobacterlum leprae Autodisplay: One-component system for efficient surface display and release of soluble recombinant proteins from Eschedchia coll IPlectroporstion efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO) Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies Codon usage tabulated from the international DNA sequence databases Signal sequences specify .-the targeting route to the endoplasmic retJculum membrane Eukaryotic protein disulfide isomerase complements Escherichie coil dsbA mutants end increases the yield of • hetarologous secreted protein with disulfide bonds Factors affecting the ability of energy functions to discriminate correct from incorrect folds Privalov PL: Intermediate states in protein folding In vivo and in vitro folding of a recombinant metalloenzyme, phosphomannose isomerase Binding, encapsulation and ejection: Substrata dynamics during a chaperonin-essisted folding reaction Role of non-native aromatic and hydrophobic interactions in the folding of hen egg white lysozyme Artificial chaperone-assisted refolding of denatured-reduced lysozyme: Modulation of the competition between renaturation and aggregation Functional antibody production using cell-free translation: Effects of protein disulfide isomersse end chaperones Growth kinetics of Escheitchie coil and expression of a recombinant protein end its isoforms under heat shock conditions Affinity-assisted in vivo folding of a secreted human peptide hormone in Escherichia coll Common principles of protein • translocation across membranes MONSSTER: A method for folding globular proteins with a small number of distance restraints Specific aggregation of partially folded polypeptide chains: The molecular basis of inclusion body composition Unfolding and refolding of Coprinus cinereus peroxides• at high pH, in urea, and at high temperature. Effect of organic and ionic additives on these processes Interactions between newly • synthesized glycoproteins, calnexin and a network of resident chaperones in the endoplasmic reticulum Chaperonin-facilitated protein folding: Optimization of rate and yield by an iterat]ve annealing mechanism Local interactions dominate folding in a simple protein model Autocatalyzed protein folding Signal sequence-dependent function of the TRAM protein during early phases of protein transport across the endoplasmic reticulum membrane High-level expression of mouse inducible nitric oxide syntheae in Escherichia coil requires coexpression with calmodulin Conformation-independent binding of monoglucosylated ribonuclease B to calnexin Protein folding -Chaperones get Hip. Curt Bio11996 Post-transcriptional and post-translational modifications Related review: Eukaryotic protein processing-endoproteolysis of precursor proteins ** Activation of the furin endoprotease is a multi-step process: requirements for acidification and internal propeptide cleavage Production of recombinant proteins in chinese hamster ovary cells overexpreasing the subtilisin-like proprotein converting enzyme furin The isoforms of the proprotein convertase 5 are sorted to different subcellular compartments High-level secretion of two antibody single chain Fv fragments by Pichia pastoris A multifunctional vector • system for heterologous expression of proteins in EscheHchia coil-expression of native and hexahistidyl fusion proteins, rapid purification of the fusion proteins, and removal o1 the fusion peptide by Kex2 protesse Proinsulin processing in the rat insulinoma cell line INS after overexpression of the endoproteases PC2 or PC3 by recombinant adenovirus Obesity and impaired prohormone processing associated with mutations in the human prohormone convertase 1 (PC1) gene RNA editing: A mechanism for gRNA-specified uridylate insertion into precursor mRNA Characterization of the nuclear export signal of human T-cell lyrnphotropic virus type 1 Rex reveals that nuclear export is mediated by position-variable hydrophobic interactions Purification and • enzymatic characterization of recombinant prohormone convertase 2: stabilization of activity by 21 kDa 7B2 Implication of the RRGD sequence of the proprotein convertase PC1 in zymogen processing and cellular trafficking Impaired fertility in mice deficient for the testicular germ-cell protease PC4 Secretion of active human mucus proteinasa inhibitor by Aspergillus niger after KEX2-1ike processing of a glucoamylase-inhibitor fusion protein Capsid targeting sequence targets foreign proteins into bacteriophage T4 and permits proteolytic processing In Vitro characterization of the novel proprotein convertase PCZ Bio-activation of MIS during • gonadal development by a kex2/subtilisin-like endoprotease Production of highly homogeneous and structurally intact recombinant yon Will•brand factor multimers by furin-mediated propeptJde removal in vitro cDNA structure, tissue distribution and chromosomal localization of rat PC7: a novel mammalian proprotein convertase closest to yeast kexin-like proteinasea Bemstein HD: The E-coil signal recognition particle is required for the insertion of a subset of inner membrane proteins Transgenic livestock =• as drug factories. SciAm Transcription-induced deletions in plasmid vectors: M13 DNA replication as a source of instability. Me/Gen Genet Periplasmic expression of part of the major rotavirus capsid protein VP7 containing all the three antigenic regions in Escberichis coil Gene Expression of the Zymomonas mobilis gfo gene for NADP-containing glucoserfructose oxidoreductase (GFOR) in Escherichia coil -Formation of enzymatically active preGFOR but lack of processing into a stable periplasmic protein Systems for regulating gene expression, and transgenics and knockouts Related review: Inducible gene expression in mammalian cells and transgenic mice Regulatory mechanisms involved in activator-protein-1 (AP-1)-mediated activation of glutathione-S-transferase gene expression by chemical agents Osmoregulation and protein expression in a pbs2 Delta mutant of Saccharomyces cerevisiae during adaptation to hypersaline stress Genetic characterization of transactivation of the human T-cell leukemia virus type 1 promoter: Binding of tax to tax-responsive element 1 is mediated by the cyclic AMP-responsive members of the CREB/ATF family of transcription factors Co-regulation of two gene activities by tetracyline via a bidirectional promoter Characterisation of two promoters for prion protein (PrP) gene expression in neuronal cells ** Controlling protein association and subcellular localization with a synthetic ligand that induces heterodimedzation of proteins Regulated expression of a wheat germin gene in tobacco: Oxalata oxidase activity and apoplastic localization of the heterologous protein Three functional classes of transcriptional activation domains Long-term control of ** erythropoietin secretion by doxycydine in mice transplanted with engineered primary myoblssts Dynamics and modeling of temperature-regulated gene product expression in recombinant yeast fermentaUon Bi-directiooal gene switching with the tetracycline represser and a novel tetracycline antagonist Multiple promoter elements differentially regulate the expression of the mouse tenascin gene Association of increased spontaneous mutation rates with high levels of transcription in yeast A regulatory domain within the vires-response element of the interferon alpha 1 gene acts as a transcriptional represser sequence and determinant of cell-speclfic gene expression Correlation of GC content with replication timing and repair mechanisms in weakly expressed F-coli genes The transcriptional activity of a muscle-specific promoter depends critically on the structure of the TATA element and its binding protein cis-acting sequences located downstream of the human immunodeficiency virus type 1 promoter affect its chromatin structure and transcriptional activity The transcriptional regulator CPRFI: Expression analysis and gene structure. Me/Gen Genet Variegated expression of a globin transgene correlates with chromatin accessibility but not methylation status Regulation of an early developmental checkpoint in the B cell pathway by Ig beta Promoter control of translation in Xenopus oocytes Preference for guanosine at first codon position in highly expressed Escherichia coil genes. A relationship with translational efficiency Bifunctionali~ of the AcMNPV homologous region sequence (hrl): Enhancer and ori functions have different sequence requirements The myeloid-cell-specific c-fes promoter is regulated by Spl, PU.1, and a novel transcription factor Completely uncoupled and perfectly coupled gene expression in repressible systems Dimeric ligands define a role for transcriptional activation domains in reinitiation Rapid retroviral • delivery of tetracycline-inducible genes in a single autoregulatory cassette Lack of gradual regulation of tetrecycline-controlled gene expression by the tetrecyclin-repressor/VP16 transactivetor (tTA) in cultured cells Regulation of cellulase gene expression in the filamentous fungus Trichoderma teasel Transcriptional control: Versatile molecular glue The mammalian transcriptional repressor REP (CBF1) regulates interieukin-6 gene expression Effects of growth rate on the production of Pseudomonas fluorescens lipase during the fed-batch cultivation of Escherichia coil Biotechno/Prog The regulation of human immunodeflciency virus typed gene expression Influence of agitation and sperging on the growth rate and infection of insect cells in bioreactors and a comparison with hybridoma culture DoxycycHne-medieted quantitative and tissue-specific control of gena expression in transgenic mice A novel transcriptional activator originating from an upstream promoter in the human growth hormone 9ene Function of 3' non-coding sequences and stop codon usage in expression of the chloroplast psaB gene in Chlamydomones reinhardtii An 18-base-pair sequence in the mouse pro alpha 1(11) collagen gene is sufficient for expression in cartilage and binds nuclear proteins that are selectively expressed in chondrocytes Strength of translation initiation signal sequence of mRNA as studied by quantification method: Effect of noclentide substitutions upon translation efficiency in rat preproinsulin mRNA IL-16 represses HIV-1 promoter activity The human Cut homeodomeln protein can repress gene expression by two distinct mechanisms: Active repression and competition for binding site occupancy Downregulation of RAR alpha in mice by antisense transgene leads to a compensatory increase in RAR beta and RAR gamma and development of lymphome Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae Control of memory formation through regulated expression of a CaMKII transgene Distinct gene expression patterns in skeletal end cardiac muscle are dependent on common regulatory sequences in the MLCI/3 locus Transcription-coupled repair deficiency and mutations in human mismatch repair genes ZBP-09, a Kruppel-like zinc finger protein, inhibits epidermal growth factor induction of the gastrin promoter Translation is enhanced after silent nucleotide substitutions in A+T-rich sequences of the coding region of CD46 eDNA Codon usage tabulated from the international DNA sequence databases Ecdysone-inducible gene • . expression in mammalian cells and transgenic mice Construction end characterization of a one-plasmid system for the controlled expression of genes in mammalian cells by tetracycline Use of a modified alcohol dehydrogenase, ADHI, promoter in construcbon of diecetyl non-producing brewer's yeast • , Self-contained, tetracycline-regulated retroviral vector system for 9ene delivery to mammalian cells Mechanism of protein access to specific DNA sequences in chromatin: A dynamic equilibrium model for gene regulation The MHC-encoded TAP1/LMP2 bidirectional promoter is down-regulated in highly oncogenic adenovirus type 12 transformed cells P300 is required for MyoD-dependent cell cycle arrest and muscle-specific gene transcription In vitro display of DNA elements that modulate the RNA transcription in cis or in trans directions A limited upstream region of the human sucrsse-isomeltasa gene confers glucose-regulated expression on a haterologous gene Efficiency of different viral promoters in directing gene expression in mammalian cells: Effect of 3'-untranslated sequences The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression Constitutive activation of Epstein-Berr virus (EBV) nuclear antigen 1 gene transcription by IRF1 and IRF2 during restricted EBV latency./vlol Cell Bio/ 199 Host-cell-datermined methylation of specific Epstein-Ban" virus promoters regulates the choice between distinct viral latency programs The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco Formation of recombinant proteins in Escherichia coil under control of a nitrogen regulated promoter at low and high cell densities • • Efficient control of gene expression by single step integration of the tetracycline system in transgenic mice Switching on gene expression Problems of translating heterologous genes in expression systems: The role of tRNA cis-Acting elements involved in transcripUonal regulation of the herpes simplex virus type 1 latency-associated promoter 1 (LAP1) in vitro and in vivo Catabollte-repressor-like protein regulates the expression of a gene under the control of the Escherichia coil lac promoter in the plant pathogen Xanthomonas campestris pv campestris Creating conditional mutations in • mammals Hormone-and endotoxin-modulated gene expression of a long-term organ culture system of adult rat liver Synthetic enhancement of • TFIIB defect by a mutation in SSU72, an essential yeast gene encoding a novel protein that affects transcription start site selection in vivo The use of dilution rate cycling to stabilise recombinant plasmids in continuous culture of recombinant Seccheromyces cerevisiee Vector for IS element entrapment end functional characterization based on turning on expression of distal promotarless genes Identification of functional positive end negative thyroid hormone-responslve elements in the rat epolipoproteln AI promoter Characterization of human protein C 9ene promoter: Insights from natural human mutants Structural changes in the 530 loop of Escherichie coil 16S rRNA in mutants with impaired translational fidelity cis-Acting elements that mediate the negative regulation of moloney murine leukemia virus in mouse early embryos Potent end selective inhibition of gene expression by an entisense heptanucleotide Interferon regulatory factors and TRIB cooperatively regulate interferon-responsive promoter activity in vivo and in vitro Transgenic studies with a keratin promoter-driven growth hormone transgene: Prospects for gene therapy Ligand-inducible and liver-specific target gene expression in transgenic mice The nuclear factor YY1 suppresses the human gamma interferon promoter through two mechanisms: Inhibition of AP1 binding and activation of a silencer elemenL InserUonel mutagenesis in zebratish identifies two novel genes, pescadillo and dead eye, essential for embryonic developmenL A 91ucocorticoid-medietad *-transcriptional induction system in transgenic plants Localization of protein implicated in establishment of cell type to sites of asymmetric division Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development Novel features of Drosophila period transcription revealed by real-time lucifersse reporting Colicins B and la as novel counterselective agents in interspecies conjugal DNA transfers from colic/n-sensitive Escherichia col/donors to other Gram-negative recipient species A transposon for green fluorescent protein transcriptional fusions: Application for bacterial transport experiments Expression of the green fluorescent protein-eocoding gene from a tobacco virus-based vector Expression of bacterial mUD in Sacchammyces cerevisiee results in mannitol synthesis and protects a glycerol-defective mutant from high-salt and oxidative stress Engineered GFP as • vital reporter in plants Ectopic expression of murine TPO receptor (c-mpl) in mice is pathogenic and induces erythroblestic proliferation Liver failure and defective hepltocyta regeneration in interleukin-6-deficient mice Chemical control of gene expression Yoder Jh Lc as a non-destructive visual reporter end transposition excision marker gene for tomato Removal • • of a cryptic intron and subcellular localization of green fluorescent protein are required to mark trsnsgenic Arabidopsis plants brightly Engineering green fluorescent protein • • for improved brightness, longer wavelengths and fluorescence resonance energy transfer A veccinia virus transfer vector using a GUS reporter gene inserted into the 14L locus Ruorescent differential display analysis of gene expression in differentiating neuroblsstoma cells • • Exchange of protein molecules through connections between higher plant plastids A new cat reporter gene vector designed for rapid and efficient cloning of PCR products Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products Retroviral • transfer and expression of a humanized, red-shifted green fluorescent protein gene into human tumor cells A sensitive reporter gene system using bacterial lucltersse based on a series of plasmid cloning vectors compatible with derivatives of pER322 Evaluation of tyrosinase minigene co-injection as a marker for genetic manipulation in transgenic mice improvement of Arabidopsis mutant • • screens based luciferese imaging in plants Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lent/viral vector High levels of ripening-specific reporter gene expression directed by tomato fruit polygalacturonase gene-flanking regions Crystal structure of the Aequorea victoda green fluorescent protein. Science Distribution of tobamovirus movement in infected cells and implications for cell-to-cell spread of infection Ruorescence resonance • • energy transfer (FRET) imaging of a single living cell using green fluorescence protein Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II Long-range disruption of gene expression by a selectable marker cassette Green fluorescent protein and its derivatives as versatile markers for gene expression in living Drosophila, plant and mammalian cells Expression of functional recombinant human procathepsin B in mammalian cells Double labeling of subcellular structures with organelle-targeted GFP mutants in vivo Detection in • • living cells of Ca2+-dependent changes in the fluorescence emission of a indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence From screens to genes: Prospects for inserfional mutagenesis in zabrafish Vectors using the phospho-alpha-(1,1)-glucosidase-encoding gene treA of Bacillus subtilis as a reporter Activation of the human estrogen receptor by estrogenic end ant/estrogenic compounds in Saccheromyces carevisiae: A positive selection system Mutations • that suppress the thermosensitivity of green fluorescent protein The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas Quantitative analysis of • transient gene expression in mammalian cells using the green fluorescent protein Selective production of transgenic mice using green fluorescent protein as a marker Visualization of G-protein-coupled receptor trafficking with the aid of the green fluorescent protein CD2 antigen expression on leukemic cells as a predictor of event-tree survival after chemotherapy for T-lineage acute lymphoblestic leukemia: A Children's Cancer Group study The viral thymidine kinase gene as • tool for the study of mutagenesis in Trypanosoma bruce Improved thermostability of the North American firefly luciferase: Saturation mutagenesis at position 354 The molecular structure • • of green fluorescent protein Spatial dynamics of GFP-tagged • proteins investigated by local fluorescence enhancement A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells Genetic engineering of metabolic pathways Related review: Expression of drug-metabolizing enzymes A: Improving production of aromatic compounds in Escherichle col/by metabolic engineering Coexpression of a human P450 (CYP3A4) and P450 reductase generates, a highly functional monooxygenase system in Escherichia coll Identification of retained N-formylmethionine in bacterial recombinant cytochrome P450 proteins with the N-terminal sequence MALLLAVFL-.: roles of residues 3-5 in retention end membrane topology Recombinant DNA technology as • an investigative tool in drug metabolism research Eukaryotic • expression systems: a comparison Development of an in situ toxicity assay system using recombinant baculoviruses Biotransformation New applications • of bacterial systems to problems in toxicology Purification • of recombinant human cytochrome P450 enzymes expressed in bacteria Modification of biochemical pathways in industrial yeasts Multiple forms of human P450 expressed in Saccharomyces cerevisiae: systematic characterization and comparison with those of the raL Biochem Pharmaco11996 Philpot RM: Expression .• and characterization of a flay/n-containing monooxygenase 4 from humans Metabolic pathway engineering in cotton: Biosynthesis of polyhydroxybutyrate in fiber cells Establishment of transgenic mice carrying human fetus-specific CYP3A7 Making cells work -Metabolic engineering for everyone Baculovirus-directed expression of • rabbit UDP-glucuronosyitrensferases in Spodoptere /rug/pards cells A single amino acid change in a pathway-specific transcription factor results in differing degrees of constitutivity, hyperinducibility and derepression of several structural genes Catalysis of • • reactions important in drug metabolism by Escherichia coil expressing human cytochromes P450 Molecular engineering study on electron transfer from NADPH-P450 raductase to rat mitochondriel P450c27 in yeast microsomes Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespirstis biosynthetic genes The function • • of recombinant cytochrome P450s in intact Eschenchia con cells: the 17ct-hydroxylation of progesterone and pregnenolone by P450clZ Arch Biochem Biophys 199? Putidaredoxin reductase-putidaredoxin-cytochrome P450ca m triple fusion protein. Construction of a salt-sufficient Escherichia coil catalytic system Genetic engineering of plant secondary metabolism -Accumulation of 4-hydroxybenzoate glucosides as a result of the expression of the bectedal ubiC gene in tobacco Synthetic modification of the Escherichia coil chromosome: Enhancing the biocatalytic conversion of glucose into aromatic chemicals Engineering pathways for malate degradation in Saccharomyces cerevisiae • Microsornal P450 2C3 is expressed as a soluble dimer in Escherichia coil following modifications of its N-tarminus • Arylamine N-acetyitransferases. Expression in EscheHchis coil, purification, and substrata specificities of recombinant hamster monomorphic and polymorphic isozymes Optimized • haterologous expression of the polymorphic human glutsthione transferase M1-1 based on silent mutations in the corresponding cDNA Gene expression in nematode and mammalian cells Related review: Plasmid DNA expression systems for the purpose of immunization In Xenopus oocytes the human C3a and CSa receptors elicit a promiscuous response to the snaphylatoxins Structural cheracterisation of human recombinant glycohormones follitropin, lutropin and choriogonadofropin expressed in Chinese hamster ovary cells A source of glycosylated human T-call lymphotropic virus type 1 envelope protein: Expression of gp46 by the vaccinia virus T7 polymerase system Gene cassettes for directional insertion at the Sfil cleavage site in the SV40 replication origin of mammalian expression vectors Controlled gene expression in mammalian cells via a regulatory cascade involving the tetracycline transactivstor and lac repressor Molecular pathogenesis of type I congenital plasminogen deficiency: Expression of recombinant human mutant plasminogens in mammalian cells Expression of Fas/CD95 and Bcl-2 by primitive hematopoieUc progenitors freshly isolated from human fetal liver Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation Regulation of in vitro gene expression using antisense oligonucleotides or antisense expression plasmids transfected using stsrburst PAMAM dendrimers T lymphocyte-directed gene therapy for ADA(-) SCID: Initial trial results after 4 years Gene therapy in peripheral blood lymphocytes and bone marrow for ADA(-) immunodoficient patients Protection of chimpanzees from high-dose haterologous HIV-1 challenge by DNA vaccination Intron-less RNA injected into the nucleus of Xenopus oocytes accesses a regulated translation control pathway Gene transfer to hematopoietic cells Site-directed mutageneais of Cys-15 and Cys-20 of pulmonary surfactsnt protein d -Expression of a trimeric protein with altered anti-viral properties Recovery of infectious respiratory syncytial virus expressing an additional, foreign gene Characterization of the human cytochrome P4502D6 promoter -A potential role for antagonistic interactions between members of the nuclear receptor family Inducible retrovirst vectors regulated by lec repressor in mammalian cells A functionally defective allele of TAP1 results in loss of MHC class I antigen presentation in a human lung cancer Regulated secretion of prolactin by the mouse insulinoma cell line bets TC-3. Biotechno/ogy Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells Selective isolation of transiently transfected cells from a mammalian cell population with vectors expressing a membrane anchored single-chain antibody Expression of the mouse msstocytoms glocosaminyl N-deacatylasa N-sulfotransferase in human kidney 293 cells results in increased N-sulfation of heperan sulfate The bets-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates A positive regulatory sequence of hepatitis B viral small X promoter The Ray-responsive element negatively regulates human immunodoficiency virus type 1 env mRNA expression in primate cells InducSon of • cytotoxic T lymphocytes and an6tumor immunity with DNA vaccines expressing single T cell epitopes A replication-competent promofer-trap retrovinJs Transfer of genes to humans: Early lessons end obstacles to success Deletions at short direct repeats and base substitutions are characteristic mutations for bleomycin-induced double-and single-strand breaks respectively, in a human shuttle vector system Hemopoiesis and Gene Therapy Purcell RH: • DNA vaccine for hepatitis B: evidence for immunogenicity in chimpanzees and comparison with other vaccines Isolation of AngiopoieUn-1, a ligand for the TIE.?. receptor, by secretion-trap expression cloning Molecular mechanisms governing tumor-necrosis-factor-mediated regulation of plasminogen-activator inhibitor type-2 gene expression Variegated transgene expression in mouse mammary gland is determined by the transgene integraUon locus Targeted gene delivery by tropism-modified adenovirel vectors Endoplasmic reticulum protein expression in recombinant NSO mystomss grown in botch culture Candidacidst activity of human salivary histstin recombinant variants produced by site-directed mutagenesis cis-acting sequences located downstream of the human immunodoficiency virus type 1 promoter affect its chromatin structure and transcriptional activity Ectopic expression of the ewthropoietin receptor in a routine interleukin-6-dependent plasrnacytoma cell line (TEPC-2027) confers proliferative responsiveness to erythropoietin Adenovirus dodecahedron, a new vector for human gene transfer Geller Ah Helper virus-free transfer of herpes simplex virus type 1 plasmid vectors into neural cells The histone 3'-terminal stem-loop is necessary for translation in Chinese hamster ovary cells Biology of adenovirus vectors with E1 and E4 deletions for liver-directed 9ene therapy Mutsgenicity of a unique thymine-thymine dimer or thymine-thymine pyrimidine pyrimidone (6-4) photoproduct in mammalian cells Two versatile eukaryotic expression vectors permitting epitope tsgging, radiolabelling and nuclear Iocalisation of expressed proteins GlycosylatJon of recombinant ancrod from Agkistrodon rhodostoma offer expression in mouse epithelial cells Reduction of metastatic properties of BL6 melanoma cells expressing terminal fucose alpha 1-2-galactose after alpha 1,2-fucosyttransterase cDNA transtection Removal of sialic acid from a glycoprotein in CHO cell culture supernatant Expression systems Gene expression in nematode and mammalian ceils by action of an extrecelluler CHO cell sialidase Construction, replication, and immunogenic properties of a Simian immunodaficiency virus expressing intedeukin-2 Kingsman SM: Promoter control of translation in Xenopus oocytes Transport of DNA into the nuclei of Xenopus oocytes by a modified VirE2 protein of Agrobacterium A non-expressed transgane as a ceil marker for the investigation of ceiiuier traffic after splenic autotransplantation Expression of the plasma membrane Ca2+-ATPase in myogenic cells High-level globin gene expression mediated by a recombinant adeno-associated virus genome that contains the 3' gamma globin gene regulatory element and integrates as tandem copies in erythroid cells Formation of de novo centromeres and construction of first-generation human artificial microchromosomes Gane conversion during vector insertion in embryonic stem cells Construction of adenovirsl and retroviral vectors coexpressing the genes encoding the hepatitis B surface antigen and B7-1 protein Molecular characterization of replication-competent variants of adenovirus vectors and genoma modifications to prevent their occurrence A novel tetrecycline-dependent expression vector with low basal expression and potent regulatory properties in various mammalian cell lines Enzymatic characterization of hepatitis C virus NS3/4A complexes expressed in mammalian cells by using the herpes simplex virus amplicon system Lack of gradual regulation of tetracycline-controlled gene expression by the tetrecyclin-repressor/VP16 transactivator (tTA) in cultured cells Plasmid DNA expressing a secreted or a nonsecrated form of hepatitis C virus nucleocapsid: Comparative studies of antibody and T-helper responses following genetic immunization N-glycosylation of recombinant human interteron-gamma produced in different animal expression systems Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase Overexpression of catalytic subunit pl 10 alpha of phosphatidylinositol 3-kinase increases glucose transport activity with translocation of glucose transporters in 3T3-L1 adipocytee Molecular cloning of a novel mRNA highly expressed in haemochromatotic human liver and proliferating cells Cap-independent translation initiation in Xenopus oocytes Sakakl Y: Ruorescent differential display analysis of gene expression in differentiating nauroblastoma cells Transcriptional regulation of the human iNOS gene in vascular-smooth-muscle cells end macrophages: Evidence for tissue specificity Expression of peptides encoded by exons in cloned mammalian DNA Simplified plasmid rescue of host sequences adjacent to integrated proviruses Production of the R2 subunit of ribonucleotide reductase fTom herpes simplex virus with prokaryotic end aukaryotic expression systems: Higher activity of R2 produced by eukaryotic cells related to higher iron-binding capacity An autonomously replicating eukaryotic expression vector with a tetracycline-responsive promoter Characterization of two recombinant PDE3 (cGMP-Inhibited cyclic nucleotide phosphodiesterase) isoforms, RcGIP1 and HcGIP2, expressed in NIH 3006 murine fibroblasts and Sf9 insect cells Recombinant adenoviruses with large deletions generated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo~ J Viro11996 Constitutive retinoid receptors expressed from adenovirus vectors that specifically activate chromosomal target genes required for differentiation of promyelocytic leukemia and teratocarcinoma cells Fodstad O: Reversal of the in vivo metastatic phenotypa o1 human tumor cells by an enti-CAPL (rots1) ribozyme Mammary gland expression o1 transgenes and the potential for altering the properties of milk DNA-mediated immunization in a transgenic mouse model of the hepatitis B surface antigen chronic carder state Induction of antigen-presenting capacity in tumor cells upon infection with non-replicating recombinant vaccinia virus encoding murine MHC class II and costimulatory molecules Improved adenovirus vector provides herpes simplex virus ribonucleotide reductase R1 and R2 subunits very efficiently Production and characterization of recombinant active mouse gelatinase B from eukaryotic cells and in vivo effects after intravenous administration Ectopic expression of CHOP (GADD153) induces apoptosis in M1 myeloblastic leukemia cells E-cadherin expression in human breast cancer cells suppresses the development of osteolytic bone metastases in an experimental metastasis model Inactivation of I kappa B beta by the Tax protein of human T-cell leukemia virus type 1: A potential mechanism for constitutive induction of NF-kappa B Efficient transfer of regulated genes in adipocytes and hepatoma cells by the combination of liposomas and replication-deficient adenovirus Miller AD: Cell-surtace receptors for retroviruses and implications for gene transfer Retrovirus packaging cells based on 10A1 murine leukemia virus for production of vectors that use multiple receptors for cell entry Gene expression and cell fusion analyzed by lacZ complementation in mammalian cells Expression of recombinant human granulocyte colony-stimulating factor in CHO dbfr(-) cells: New insights into the in vitro amplification expression system Genetically engineered poxviruses for recombinant gane expression, vaccination, and safety Adjacent, cooperative elements form a strong, constitutive enhancer in the human granulocyte-mecrophage colony-stimulating factor gene Construction and characterization of a one-plasmid system for the controlled expression of genes in mammalian cells by tetracycline MR: Identification of previously unrecognized common elements in eukaryotic promoters -A ribosomal RNA gene initiator element for RNA polymerase I Recombinant protein synthesis in Chinese hamster ovary cells using a veccinia virus/bacteriophage "r7 hybrid expression system Preferential induction of a Tht immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization Structure and function in rhodopsin: High level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines CACCC and GATA-1 sequences make the constitutively expressed alpha-globin gene en/throid-responsive in mouse erythroleukemia cells Suppression of trk(B) expression by antisense oligonucleotides alters a neuronal phenotype in the rod pathway of the developing rat retina Efficiency of different viral promoters in directing gene expression in mammalian cells: Effect of 3'-untranslated sequences Immunostimulatory DNA sequences necessary for effective intredermal gene immunization Cloning and partial characterization of the mouse glufamine.'fructose-6 -phosphata amidotransferase (GFAT) gene promoter Overexpreseion of recombinant human antithrombin III in Chinese hamster ovary cells results in malformation and decreased secretion of recombinant protein Foreign • (M13) DNA ingested by mice reaches pedpherei leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalantly linked to mouse DNA The use of the rat LAP LCR and promoter for the high-level constitutive expression of K+ channel cDNAs in a rat liver cell line Novel exploitation of s nuclear function by influenza virus: The cellular SF2/ASF splicing factor controls the amount of the essential viral M2 ion channel protein in infected cells A shutie system for transfer of YACs between yeast end mammalian cells Stable episomal maintenance of yeast artificial chromosomes in human cells. Mo/Ce/I Bio11996 CliO DUKX cell lineages preadaptad to growth in serum-free suspension culture enable rapid development of cell culture processes for the manufacture of recombinant proteins Adenovirus EIA proteins inhibit activation of transcription by p53 Stable expression in HEK-293 cells of the rat alpha 3/beta 4 subtype of neuronal nicotinic ecetylcholine reCel~t~ Hematopolatic stem ceil-based 9ene therapy for acquired immunodeficiency syndrome: Effident transduction and expression of RevM10 in myeloid cells in vivo end in vitro Testing the eukaryotic promoters for efficient expression of exogenous genes in chondrocytes and synoviocytes The thrombin receptor is present in myoblasts and its expression is repressed upon fusion beta-thymosins are not simple actin monomer buffedng proteins -Insights from overexpreasion studies Broad host range of human T-ceil leukemia virus type 1 demonstrated with an improved pseudotyping system Stability of the human dystrophin transcript in muscle pHIVTATA-CAT, a versatile vector to study transcriptional regulatory elements in mammalian cells Ceil-type-specific regulation of the human tumor necrosis factor alpha gene in B cells and T cells by NFATp, and ATF-2/JUN Alternative processing of the tryptophanyl-tRNA synthetase mRNA from interferon-treated human ceils Expression and Intracellular localization of catechol O-mathyltransferese in trensfected mammalian cells • Generation of MHC class-I restricted T lymphocytes by expression of a viral protein in muscle cells: antigen presentation by non-muscle cells. /mmuno/ogy Overexproesion of human poly(ADP-ribose) polymersse in transfected hamster cells leads to increased poly(ADP-ribosyl)stJon end cellular sensitization to gamma irradiation Intreceilular expression of RNA transcripts complementary to the human immunodeficiency virus type 1 gag gene inhibits viral replication in human CD4(+) lymphocytes Regulation of replicon size in Xenopus egg extracts Development of a VSV-G protein pseudotyped retrovirsl vector system expressing dominant oncogenes from a lecO-modified inducible LTR promoter A hybrid herpesvirus infectious vector based on Epstein-Barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo Immune response • to a neonatal genetic immunization. Viro/ogy Reversible immortalization of mammalian cells mediated by retrovirsl transfer and site-specific recombination Adenovirus targeted to heparan-contsining receptors increases its gene delivery efficiency to multiple cell types Human herpesvirus 7 infection of lymphoid and myeloid cell lines transduced with an adenovirus vector containing the CD4 gene Spf binding plays a critical role in Erb-B2-and v-res-mediated downregulation of eipha(2)-integdn expression in human mammary epithelial cells Complex formation between CREB and tax enhances the binding affinity of CREB for the human T-cell leukemia virus type 1 21 -base-peir repeats The axis-inducing activity, stability, and subcellular distribution of bets-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3 Transcriptional activation of a retrovirus enhancer by CBF (AML1) requires a second factor: Evidence for cooperativity with c-Myb Identification of a promoter that controls aromatsse expression in human breast cancer and adipose stromal cells Development of a complementing cell line and a system for construction of adenovirus vectors with E1 and E2a deleted Estrogen and thyroid hormone interaction on regulation of gene expression A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells Gene fusions High-level misincorporation of lysine for arginine at AGA codons in a fusion protein expressed in Escherichia coiL Engineering of • recombinant colorlmetric fusion protein for immunodiagnosis of insulin Production of recombinant bovine enterokinase catalytic subunit in Escherichia coil using the novel secretory fusion partner DsbA Vil[averde A: The position of the heterologous domain can influence the solubility and proteolysis of beta-geiactosidase fusion proteins in E-coll A modular set of FIp, FRT and lacZ fusion vectors for manipulating genes by site-specific recombination A multifunctional vector system for heterologous expression of proteins in Escherichie coil -Expression of native and hexahistidyl fusion proteins, rapid purification of the fusion proteins, and removal of fusion peptide by Kex2 protease Introducing Stul sites improves vectors for the expression of fusion proteins with factor Xa cleavage sites A miniature bioreactor for sensing toxicity using recombinant bioluminescent Escherichia coil cells Genomic targeting with an MBP-Cre fusion protein Extending filamentous phage host range by the grafting of a heterologous receptor binding domain A lacZ-hygromycin fusion gene and its use in a gene trap vector for marking embryonic stem cells Biophysical characterization of recombinant proteins expressing the leucine zipper-like domain of the human immunodeficiency virus type 1 transmembrane protein gp41 Identification of functional domains within the alpha and beta subunits of beta-hexosaminidase A through the expression of alpha-beta fusion proteins Polylinker-encoded peptides can confer toxicity to recombinant proteins produced in Escherichla coll Investigating the use of the chymosin-sensitive sequence of kappa-casein as a cleavable linker site in fusion proteins Detection of tetramerization domains in vivo by cooperative DNA binding to tandem lambda operator sites A new expression vector for high level protein production, one step purification and direct isotopic labeling of calmodulin-binding peptide fusion proteins Attenuation of gene expression by a tHnucleotide repeat-rich tract from the terminal exon of the rat hepatic polymeric immunoglobulin receptor gene Alphaviruses as vectors for gene delivery Protection against myxomatosis and rabbit viral hemorrhagic disease with recombinant myxoma viruses expressing rabbit hemorrhagic disease virus capsid protein Protein translocation -Rehearsing the ABCs The promoter for tomato 3-hydroxy-3-methylglutaryl coenzyme s reductase gene 2 has unusual regulatory elements that direct high-level expression Targeting of two Arabidopsis H+-ATPase isoforms to the plasma membrane The cooperation between two silencers creates an enhancer element that controls both the lens-preferred and the differentiation stage-specific expression of the rat beta B2-crystallin gene A 5" differentially methyleted sequence and the 3'-flanking region are necessary for H19 trensgene imprinting A fiber-optic DNA biosensor microarray for the analysis of gene expression Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts Retroviral vectors designed for targeted expression of RNA polymerase Ill-driven transcripts: A comparative study High-level secretion of human alpha(1)-antitrypsin from Saccharomyces cerevisiae using inulinase signal sequence A highly efficient cell-free protein synthesis system from Escherichia coil Cooperetivity of stabilized mRNA and enhanced translation activity in the ceil-free system A new adenoviral vector: Replacement of all viral coding sequences with 28 kb of DNA independently expressing both full-length dystrophin and beta-galactosidase Rapid screening and mapping of conformetional epitopes expressed in the secretion expression system Pichie pastoris Therapeutic levels of functional human factor X in rata after retroviral-medieted hepatic gene therapy Nitrogen mustard inhibits transcription and translation in • cell free system A novel periplasmic carrier protein involved in the sorting and transport of Escherichia cola lipoproteins destined for the outer membrane Highly stable expression of a foreign gene from rabies virus vectors RNA-medieted RNA degradation and chalcone synthase A silencing in Petunia. Ce//199? Plants as bioreactors for biopharmaceuticals: Regulatory considerations. Trends Biotechno/199'7 Highly productive ceil-free protein synthesis system using condensed wheet-germ extract The pCL vector system: Rapid production of helper-free, high-tater, recombinant retroviruses Influence of DNA sequence identity on efficiency of targeted gene replacement Expression of functional elements inserted into the 35S promoter region of infectious cauliflower mosaic virus replicons Genetically engineered resistance to dengue-2 virus transmission in mosquitoes A cooperative interaction of human T-Cell leukemia virus type 1 tax with the p21 cyclin-dependent kinase inhibitor activates the human immunodeticiency virus type 1 enhancer Zurcher T, Palese P, (3arcissastre A: A plasmid-based reverse genetics system for influenza A virus Screening differentially expressed cDNA clones obtained by differential display using amplified RNA Protein secretion: Sorting sweet sorting High-tater bicistronic retroviral vectors employing foot-and-mouth disease virus internal ribosome entry site Protein sorting by transport vesicles Quantitative monitoring of gene expression patterns with a complementary DNA microarray Rapid determination of plasmid copy number Development of an immunorediometric essay for quantitative determination of CrylA(b) protein in transgenic sugarcane plants Serial analysis of gene expression Genetic correction of p67(phox) deficient chronic grenulometous disease using peripheral blood progenitor cells as • target for reti'ovirus mediated gene transfer Drosophila metanogester angiotensin I-converting enzyme expressed in Pichia pestoris resembles the C domain of the mammalian homologue and does not require glycosylatJon for secretion and enzymic activity