key: cord-0008232-9ultha80 authors: Xing, Gang; Gu, Jinyan; Yan, Liping; Lei, Jing; Lai, Alexander; Su, Shuo; Zhou, Jiyong title: Human infections by avian influenza virus H5N6: Increasing risk by dynamic reassortment? date: 2016-04-16 journal: Infect Genet Evol DOI: 10.1016/j.meegid.2016.04.009 sha: dabadb2914e01a978a1cb2b79c30d08b7a5e4a38 doc_id: 8232 cord_uid: 9ultha80 nan Human infections by avian influenza virus H5N6: Increasing risk by dynamic reassortment? To the Editor, Despite the increased surveillance and control by vaccination, avian influenza virus (AIV) remains enzootic in domestic poultry in China and in many Asian and countries (Guan et al. 2009; Huang et al. 2016) , and infected poultry remains the source for human infections. As of 20 January 2016, the World Health Organization (WHO) has reported 846 laboratory-confirmed human infections with highly pathogenic avian influenza HPAI H5N1 across 16 countries resulting in 449 deaths since 2003 (http://www.who.int/influenza/human_animal_interface/ Influenza_Summary_IRA_HA_interface_20_Jan_2016.pdf?ua=1 ), indicating that HPAI remains a significant public health threat (Peiris et al. 2007 ). In addition, increasing number of subtypes other than H5N1 and H9N2 had emerged, e.g., H5N2, H5N6, H5N8, and H7N9, a result of reassortment either among enzootic viruses in poultry, or with viruses carried by migratory birds. Conditions conducive to this "dynamic reassortment" (continuous reassortment/process involving) include poultry trade and mixing of host species in live poultry markets (LPM). A novel AIV H5N6, with the HA from a variant of the Asian HPAI H5 lineage, was first identified in Laos, Vietnam, and China in 2013 (Reassortant Highly Pathogenic Influenza A(H5N6) Virus in Laos; Mok et al. 2015) . This virus has caused epizootics in poultry and at least nine human infections (Table S1) ,with the earlier three deaths reported in the southwest China while the most recent five cases all occurring in the Guangdong Province. Of concern, four additional human infections by AIV H5N6 were reported in January 2016 alone (http:// www.who.int/csr/don/archive/country/chn/en/). On genetic analysis of human isolates, generation of novel H5N6 viruses by "dynamic reassortment" is best illustrated in Fig. 1 . As shown, H5N6 isolates from human cases in Sichuan was caused by a reassortant virus with the HA gene from a clade 2.3.4.4 Sichuan-like H5N1 virus (nomenclature according to ), and the NA and other internal genes from a H6N6 virus and a virus of clade 2.3.2.1 H5N1 virus, respectively. Likewise, the Guangzhou case was caused by a reassortant virus with the HA originated from a Guangdong-Jiangxi-like clade 2.3.4.4 H5N1 virus Pan et al. 2016; Yang et al. 2015) . The progenitor H5N1 viruses were from ducks. However, the two human cases in 2015 were caused by a new H5N6 reassortant, of which the HA and NA genes were from Guangzhou-like H5N6 virus, but the internal genes were from H7N9/H9N2 in chickens. (Data source: A/Yunnan/14563/ Infection, Genetics and Evolution 42 (2016) 46-48 2015 and A/Yunnan/14564/2015 ; and Shenzhen http:// www.szcdc.net/jkdt/zxdt/yqcl/201601/t20160103_3417204.htm). These clinical isolates have high sequence identity to viruses detected in poultry farm and in LPMs Pan et al. 2016; Qi et al., 2014) (http://www.szcdc.net/jkdt/zxdt/yqcl/201601/t20160103_ 3417204.htm.). Clinical history suggests that LPMs were the source of some of these human H5N6 infections. This is not unexpected, as the most significant factor for transmission and dissemination of AIV in China is by live poultry trade and LPMs (Su et al. 2015; Su et al. 2014) , posing a risk for the general public. This dynamic reassortment provides a new venue to study the virulence of HPAI H5NX, and it should be a priority for further research. Of note, retention of internal genes from H7N9/H9N2 in human cases of H5N6, H7N9, and H10N8 infections has been observed (Liu et al. 2014; Su et al. 2015; Xu et al. 2016) , but the contribution of these internal genes to virulence remains to be elucidated. In view of these emerging H5N6 cases and the continuing human infections by AIV H7N9, a better understanding of the circulation and evolution of AIV in southern China is urgently needed (http://www.flu.org.cn/en/news_detail?action= ql&uid=&pd=&newsId=18492). Of note, a recently report shows that a novel reassortant H5N6 virus isolated from a girl patient in Changsha, in February, 2014 was a ternary reassortant virus by A/duck/Sichuan/ NCXN11/2014(H5N1), A/chicken/Jiangxi/12782/2014(H10N6) and a H5N2 virus isolated in duck from Jiangxi, China . Dense surveillance of these reassortant H5N6 viruses in southern China should be considered for public health hidden danger. Similar to the prevention of coronavirus reassortant in China (Su et al. 2016) , a reevaluation of current protocol for controlling AIV spread, including surveillance, vaccination, and risk assessment, is warranted. Supplementary data to this article can be found online at http://dx. doi.org/10.1016/j.meegid.2016.04.009. 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Epidemiology, evolution, and recent outbreaks of avian influenza virus in China Genetic Recombination, and Pathogenesis of Coronaviruses Trends in microbiology A fatal case of infection with a further reassortant, highly pathogenic avian influenza (HPAI) H5N6 virus in Yunnan Human infection with a novel avian influenza A(H5N6) virus Clinical, epidemiological and virological characteristics of the first detected human case of avian influenza A(H5N6) virus Gang Xing 1 Different colors represent different genetic origins. A timeline of emergence of H5N6 human cases in China is represented as a vertical line/arrow on the right This paper was supported in part by the National Key Technology R&D Program of China (2015BAD12B00), and by the Priority Academic Program Development of Jiangsu Higher Education Institutions.