key: cord-0006429-xcw0kxjg authors: Willemze, R; Rodrigues, C A; Labopin, M; Sanz, G; Michel, G; Socié, G; Rio, B; Sirvent, A; Renaud, M; Madero, L; Mohty, M; Ferra, C; Garnier, F; Loiseau, P; Garcia, J; Lecchi, L; Kögler, G; Beguin, Y; Navarrete, C; Devos, T; Ionescu, I; Boudjedir, K; Herr, A-L; Gluckman, E; Rocha, V title: KIR-ligand incompatibility in the graft-versus-host direction improves outcomes after umbilical cord blood transplantation for acute leukemia date: 2009-01-08 journal: Leukemia DOI: 10.1038/leu.2008.365 sha: 585cc7e6d3b2fb27eca260a8c223ecacee28d0c5 doc_id: 6429 cord_uid: xcw0kxjg Donor killer cell immunoglobulin-like receptor (KIR)-ligand incompatibility is associated with decreased relapse incidence (RI) and improved leukemia-free survival (LFS) after haploidentical and HLA-mismatched unrelated hematopoietic stem cell transplantation. We assessed outcomes of 218 patients with acute myeloid leukemia (AML n=94) or acute lymphoblastic leukemia (n=124) in complete remission (CR) who had received a single-unit unrelated cord blood transplant (UCBT) from a KIR-ligand-compatible or -incompatible donor. Grafts were HLA-A, -B or -DRB1 matched (n=21) or mismatched (n=197). Patients and donors were categorized according to their degree of KIR-ligand compatibility in the graft-versus-host direction by determining whether or not they expressed HLA-C group 1 or 2, HLA-Bw4 or HLA-A3/-A11. Both HLA-C/-B KIR-ligand- and HLA-A-A3/-A11 KIR-ligand-incompatible UCBT showed a trend to improved LFS (P=0.09 and P=0.13, respectively). Sixty-nine donor–patient pairs were HLA-A, -B or -C KIR-ligand incompatible and 149 compatible. KIR-ligand-incompatible UCBT showed improved LFS (hazards ratio=2.05, P=0.0016) and overall survival (OS) (hazards ratio=2.0, P=0.004) and decreased RI (hazards ratio=0.53, P=0.05). These results were more evident for AML transplant recipients (2-year LFS and RI with or without KIR-ligand incompatibility 73 versus 38% (P=0.012), and 5 versus 36% (P=0.005), respectively). UCBT for acute leukemia in CR from KIR-ligand-incompatible donors is associated with decreased RI and improved LFS and OS. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/leu.2008.365) contains supplementary material, which is available to authorized users. Allogeneic hematopoietic stem cell transplantation (HSCT) from family, unrelated bone marrow or cord blood donors is a curative treatment for a considerable number of patients with acute leukemia. Major histocompatibility complex-restricted donor T cells play an important role in HLA-matched as well as HLA-mismatched HSCT by recognizing differences in minor and major histocompatibility antigens between donor and recipient. 1, 2 A novel mechanism that relies on interactions between donor natural killer (NK) cells and recipient cells was demonstrated in the setting of haploidentical family donor HSCT. [3] [4] [5] NK cells express on their surface inhibitory killer cell immunoglobulin-like receptors (KIRs) that recognize allotypic determinants ('KIR ligands') shared by certain HLA class I allele groups. KIR2DL1 recognizes HLA-C alleles with a Lys 80 residue (HLA-Cw4 and related, 'group 2' alleles), KIR2DL2 and KIR2DL3 recognize HLA-C with an Asn 80 residue (HLA-Cw3 and related, 'group 1' alleles); KIR3DL1 is the receptor for HLA-B alleles sharing the Bw4 supertypic specificity. [3] [4] [5] Finally, KIR3DL2 was shown to function as a receptor for HLA-A3/-A11 alleles when bound to Epstein-Barr virus (EBV) peptides. 6 The role of KIR and KIR ligand in the context of HSCT has been investigated over the past 10 years. In transplants that are KIR-ligand mismatched in the graft-versus-host (GvH) direction, donor NK cells expressing inhibitory KIRs, which do not recognize ligand(s) on recipient targets, are released from HLA inhibition and mediate alloreactions leading to clinically significant graft-versus-leukemia effects. Clinical studies in haploidentical [7] [8] [9] and in some (un)related donor transplants [10] [11] [12] showed that KIR-ligand incompatibilities in the GvH direction are associated with a decreased incidence of relapse and improved disease-free and overall survival (OS) after HSCT. Several unrelated donor transplant studies did not, however, report any effect. [13] [14] [15] [16] [17] Umbilical cord blood units from unrelated donors constitute another well-established source of stem cells for HSCT in patients with leukemias. The main advantages are rapid availability and the possibility to perform HLA-mismatched transplantations due to a relatively low risk of severe GvH disease (GvHD) [18] [19] [20] [21] that increases considerably the probability of finding a suitable donor. Moreover, outcomes after umbilical cord blood transplants (UCBTs) are comparable with those after unrelated bone marrow transplantation in children and adults with acute leukemias. 19, 21 We hypothesized that in the setting of HLA-mismatched UCBT, NK-cell alloreactivity might come into play and influence outcomes. From the Eurocord database we retrieved all patients who had received a single-unit UCBT for acute leukemia in complete remission (CR). We grouped transplants according to the presence or absence of KIR-ligand mismatches that were shown to trigger donor-versus-recipient NK-cell alloreactivity in haploidentical transplantation, that is mismatches for HLA-C group 1, HLA-C group 2 and the HLA-Bw4 allele group. Moreover, as HLA-A3/-A11 KIR-ligand mismatches were not encountered in haploidentical transplants, we decided to include them in our evaluation. As HLA-A3/-A11 alleles were reported to function as KIR ligands when bound to EBV peptides, we hypothesized that, in view of the frequent EBV reactivation after transplantation, EBV peptides were readily available for HLA-A3/-A11 binding, with the possible generation of HLA-A3/-A11-specific donor NK cells that were alloreactive against target cells from recipients lacking HLA-A3 and/or HLA-A11. This retrospective analysis is based on data reported to the Eurocord Registry from European and non-European centers through a standardized questionnaire concerning patient, cord blood units, disease and graft characteristics, as well as transplant outcomes. The collected information was reviewed by two physicians and checked for computer-generated errors to ensure data quality. All patients or their legal guardians gave informed consent for UCBT according to the Declaration of Helsinki. This study was approved by the Eurocord institutional review board. The following criteria were required: (1) acute leukemia in CR, (2) HLA high-resolution typing for HLA-A, HLA-B, HLA-C and -DRB1 of patients and cord blood units, or HLA-A highresolution typing for those cord blood units without complete high-resolution typing for HLA-B and/or HLA-C, (3) nonexpanded, single, unrelated cord blood unit and (4) complete clinical data during at least 3 months of follow-up. As of 1 October 2007, 218 patient-donor pairs met the eligibility criteria. Complete information on high-resolution typing for HLA-A, HLA-B, HLA-C and HLA-DRB1 was available for 218 patients and 198 donors, and sufficient information on high-resolution typing for HLA-A and either HLA-B or HLA-C was available for 20 donors to determine KIR-ligand (in)compatibility in the GvH direction. High-resolution typing of recipient and donor HLA-B and HLA-C alleles was used to segregate patients and cord blood units in the following KIR-ligand groups as described earlier by Ruggeri et al. 22 HLA-C group 1 alleles; HLA-C group 2 alleles; and HLA-Bw4 group alleles. Non-HLA-Bw4 alleles were grouped in the HLA-Bw6 allele group, which is not a KIR ligand. In addition, patients and cord blood units were grouped according to whether or not they displayed HLA-A3/-A11 alleles, which are putative KIR ligands. 6 Composition of the study group for KIR-ligand (in)compatibility in the GvH direction HLA-C group 1 or 2 alleles, which were present in the cord blood units, were missing in 41 transplant recipients; HLA-Bw4 group alleles were missing in 12 and HLA-A3/-A11 in 22 patients. Combination of the data on HLA-A, HLA-B and HLA-C of donors and recipients resulted in 69 (32%) donor-patient pairs with KIR-ligand incompatibility in the GvH direction. Fiftyone patients were HLA-C, group 1 or 2, 19 patients HLA-Bw4 and 18 patients HLA-A3/-A11 KIR-ligand mismatched in the host-versus-graft direction with the donor. Pairs in which the donor did not miss a ligand in the patient but did not possess a KIR ligand present in the recipient (n ¼ 50) were pooled with the 99 KIR-ligand-matched pairs and, for the purpose of data analyses, are henceforth referred to as the KIR-ligand-compatible or -matched group, which comprised a total of 149 (68%). The main end points were leukemia-free survival (LFS), which was defined as the time from transplantation to either first relapse or death in CR; relapse incidence (RI), defined as any morphologically proven recurrence of leukemia occurring after the allograft; and OS, defined as the time from transplantation until the date of death. Patients were censored at the time of relapse or of the last follow-up. Other end points were engraftment, non-relapse mortality (NRM) and acute or chronic GvHD. For engraftment, we considered the incidence of neutrophil recovery as defined by a neutrophil count of at least 0.5 Â 10 9 /l for three consecutive days and graft failure as no sign of neutrophil recovery as well as transient engraftment of donor cells within 60 days after transplantation. Chimerism data were available during the first 3 months after UCBT. Full donor chimerism was defined as the presence of more than 95% of the donor cells, mixed chimerism if more than 5% and less than 95% were donor cells and autologous recovery if less than 5% of cells were donor cells. Data on the methodology of chimerism detection were not available. Acute GvHD at day 100 was diagnosed and graded according to published criteria, 23 with histopathological confirmation when possible. NRM at 2 years was defined as deaths related to transplantation and not to relapse, and chronic GvHD at 2 years was diagnosed according to standard criteria 23, 24 and evaluated in patients who survived at least 100 days with sustained engraftment. Data were analyzed from the Eurocord database with the existing data as of October 2007. The duration of follow-up was the time to the last assessment for survivors. Variables related to the patient, to the disease, to the cord blood donor and to the transplant were compared (according to KIR-ligand compatibility status) using the w 2 test for categorical and the Mann-Whitney test for continuous variables. All P-values are two-sided with type-1 error rate fixed at 0.05. To analyze the outcome, we considered variables associated with the recipient (age, weight at time of transplantation, sex and cytomegalovirus (CMV) status), with the disease (acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL), first, second or subsequent CR, and separately for ALL and AML: good-, standard-or bad-risk cytogenetics), with the cord blood donor (HLA disparity, infused total nucleated cell and CD34 þ cell doses) and with the transplant (year of transplant, reduced-intensity or myeloablative conditioning regimen, use of total body irradiation, use of ATG and GvHD prophylaxis). Cumulative incidence curves were used for RI and NRM in a competing risk setting, as death and relapse are competing together. Relapse and death in remission were considered as competing events to study neutrophil recovery, and acute and chronic GvHD. 25, 26 Gray test was used for univariate comparisons. 27 Probabilities of LFS and OS were calculated using the Kaplan-Meier estimate; the two-sided log-rank test was used for univariate comparisons. Factors associated with a P-value less than 0.10 by univariate analysis were included in multivariate analyses, using Cox proportional hazards for LFS and OS, and proportional subdistribution hazard regression model of Fine and Gray 28 for acute and chronic GvHD, neutrophil recovery, RI and NRM. Then a stepwise regression was performed using a threshold of 0.05. The diagnosis (AML and ALL) was added to the final model. All tests are two sided. The type I error rate was fixed at 0.05 for the determination of factors associated with time-toevent outcomes. Statistical analyses were performed with SPSS (SPSS Inc., Chicago, IL, USA) and S-Plus (MathSoft Inc., Seattle, WA, USA) software packages. As the effects of KIR(-ligand) mismatching have been described to be associated with AML, a limited subgroup analysis was performed with respect to KIR-ligand incompatibility for patients with AML or ALL, separately. The study group comprised 218 patients, undergoing a singleunit UCBT for AML or ALL in CR between 1997 and 2007 (median 2004). The median follow-up time is 14 months. The transplantations were performed in 57 centers. The cord blood units were supplied by 29 cord blood donor banks, mostly belonging to Netcord organization. Table 1 summarizes patient, donor and transplant characteristics for the patients transplanted with donors that were KIR-ligand incompatible (n ¼ 69) or KIRligand compatible (n ¼ 149) in the GvH direction. The diagnoses included ALL and AML, with good-, standard-or bad-risk cytogenetics in the first, second or third CR. The stages of both diseases were divided equally between the two groups. However, among patients with known cytogenetics, 17 AML patients out of 18 with a KIR-ligand-incompatible donor and 33 AML patients out of 57 with a KIR-ligand-compatible donor had bad-risk cytogenetics (P ¼ 0.02). CMV status was reported in 204 patients (111 positive) and in 202 cord blood donors (11 CMV positive). Combined information is available in 187 patient-donor pairs, of which 86 were both negative and 93 were patient positive/donor negative. EBV status of patients was reported in only 74 cases (34% of all patients) of which 58 were positive (78%) and in 78 donors (36% of all donors). CMV and EBV status were equally divided between the two KIR-ligand groups. The number of HLA disparities between donors and recipients was not significantly different between the two groups. Although a number of myeloablative and reducedintensity conditioning regimens with or without ATG were used in the 57 participating transplant centers, the frequencies of myeloablative and reduced-intensity regimens and of use of ATG were not statistically different between the two KIR-ligand groups as well as other patient-, disease-, donor-and transplantrelated factors (Table 1) . Overall outcomes of the entire study group with respect to neutrophil engraftment, acute and chronic GvHD, non-relapse mortality, RI, LFS and OS are shown in Table 2 . Association of KIR-ligand incompatibility in the GvH direction with LFS, OS and RI LFS and OS. To assess the impact of KIR-ligand mismatches that were shown to trigger donor-versus-recipient NK-cell alloreactivity in haploidentical transplantation, we determined LFS at 2 years for the donor-patient combinations that were mismatched (in the GvH direction) for HLA-C and/or HLA-Bw4 KIR ligands. It was 48±9 versus 36±4% (P ¼ 0.09) for those who were KIR-ligand matched. As we had a relatively large group of 22 donor-recipient pairs that were mismatched for HLA-A3/-A11 (in the GvH direction), we were able to assess for the first time the role of this putative KIR ligand in transplantation outcomes; LFS was 62 ± 11% for the donor-patient combinations that were mismatched for HLA-A3/-A11 versus 35 ± 4% for those who were not A3/A11 mismatched (P ¼ 0.13). As both analyses demonstrated an increased LFS in an almost not overlapping fashion and as only six patients had two incompatibilities consisting of HLA-C group and/or HLA-Bw4 group incompatibilities in combination with an HLA-A3 and/or -A11 mismatch, we combined the two groups and performed a common statistical analysis. Leukemia-free survival was significantly improved in patients receiving a UCBT from an HLA-A, -B or -C KIR-ligand incompatible donor compared with those who were transplanted with an HLA-A, -B or -C KIR-ligand compatible donor (in the GvH direction). The estimates for LFS at 2 years are 55±7 and 31±4% (P ¼ 0.005), respectively, for patients with and without donor KIR-ligand incompatibility in the GvH direction ( Figure 1a) . Overall survival was also improved in patients receiving a UCBT from a KIR-ligand-incompatible donor compared with those who were transplanted with a KIR-ligand-compatible donor. The estimates for 2-year OS were 57±7 and 40±4% (P ¼ 0.02), respectively, for patients with and without donor KIRligand incompatibility in the GvH direction ( Figure 1b) . In univariate analysis, which included age, sex, CMV status, diagnosis (AML or ALL), stage of the disease, infused total number of cells or of CD34 þ cells, HLA disparities, conditioning regimen, use of total body irradiation or ATG and KIR-ligand incompatibility (in the GvH direction), only o2 HLA disparity was associated with OS (P ¼ 0.008). In multivariate analysis, KIR-ligand incompatibility was an independent risk factor associated with improved LFS and OS. Compatible HLA grafts or with one HLA disparity were also associated with improved OS ( Table 3 ). The estimates for LFS at 2 years for AML and ALL were not significantly different. Within AML as well as ALL, LFS at 2 years was not different for the patients with good-, standard-or poor-risk cytogenetics (data not shown). Relapse. The 2-year cumulative incidence of relapse was 32 ± 4% for the entire study group, and it was 37 ± 4% for the KIR-ligand-compatible group compared with 20±5% for the KIRligand-incompatible group (P ¼ 0.03) (Figure 1c) . In univariate analysis, other factors associated with RI were type of conditioning (P ¼ 0.05), use of total body irradiation (P ¼ 0.08) and ATG (P ¼ 0.09). In a multivariate analysis, myeloablative conditioning regimen (MAC) (P ¼ 0.007), two or more HLA disparities (P ¼ 0.05) and KIR-ligand incompatibility (P ¼ 0.05) were independently factors associated with a decreased risk of relapse ( Table 3) . Association of KIR-ligand incompatibility in the GvH direction with engraftment, GvHD and NRM Neutrophil recovery. Cumulative incidence of neutrophil recovery at day 60 for the entire group was 80 ± 3%, and it was 81±4% for patients with and 79±3% for those without KIR-ligand incompatibility, respectively (P ¼ 0.21). The median time to neutrophil recovery was 21 days (4-40) for KIR-ligand-incompatible transplants and 25 (6-60) days for KIR-ligand-compatible transplants. In univariate analysis, the incidence of neutrophil recovery was associated with the median number of nucleated cells infused (P ¼ 0.015) and HLA disparity (P ¼ 0.05), but not with KIR-ligand incompatibility. In a multivariate analysis (Table 2) , only a higher number of nucleated cells (43.09 Â 10 7 /kg) infused were associated with higher rates of neutrophil recovery (P ¼ 0.015). At 3 months after UCBT, chimerism data were available in 158 patients: 126 patients were full donors (80%), 15 had mixed chimerism (9%) and 17 (10%) had autologous reconstitution. There was no association between KIR-ligand incompatibility and full donor chimerism (P ¼ 0.40). On the basis of antigen-level HLA-A and HLA-B and allele-level HLA-DRB1 typing. Acute and chronic GvHD. Sixty-five patients presented grade II-IV acute GvHD (grade II, n ¼ 36, 18%; grade III, n ¼ 14, 7%; grade IV, n ¼ 15, 7%). At day 100, cumulative incidence of grade II-IV acute GvHD for the entire population was 29±3%, and it was 28 ± 5% for patients with a KIR-ligand-incompatible donor and 30±3% for patients with a KIR-ligand-compatible donor (in the GvHD direction), respectively (P ¼ 0.82). The 2-year cumulative incidence of chronic GvHD was 19% (n ¼ 29 for 171 patients at risk). There was no association of chronic GvHD with the KIR-ligand groups. NRM and non-relapse causes of death. The 2-year cumulative incidence of NRM was 30 ± 3%, and it was 31±4% for patients with a KIR-ligand-compatible donor and 25 ± 5% for those with a KIR-ligand-incompatible donor (P ¼ 0.34). In a multivariate analysis, only RIC and a lower number of HLA disparities (compatible or one HLA disparity) were associated with decreased NRM (Table 3) . Death due to opportunistic infections (viral, fungal and parasitic) was more frequent in the KIR-ligand-compatible donor group than in the KIR-ligand-incompatible donor group (Table 4) . Unfortunately, more detailed information on the nature of these lethal infections is not available in the Eurocord database, but they occurred equally in the groups of CMV-positive and CMVnegative patients. Association of KIR-ligand incompatibility in the GvH direction with outcomes in patients with AML or ALL in CR A limited subgroup analysis was performed for 94 patients with AML (47 patients in first CR; 40 patients in second CR and 7 patients in third CR) and 124 patients with ALL (58 patients in first CR, 51 patients in second CR and 15 patients in third CR). In AML UCBT recipients, 2-year cumulative incidence of relapse was associated with the presence or absence of KIRligand incompatibility in the GvH direction (5±4 versus 36 ± 7%, P ¼ 0.005). The 2-year estimates for LFS were 73±10 and 38±7% (P ¼ 0.012) and for OS were 70±11 and 36 ± 8% (P ¼ 0.016), respectively (Figure 2a) . In spite of the trend of a decreased RI and improved LFS and OS for those with a KIR-ligand-incompatible donor, there was not a significant statistical association in ALL patients (Figure 2b ). NRM and acute and chronic GvHD were not influenced by the diagnosis (AML or ALL) or by the KIR-ligand-compatibility status. This study demonstrates that inhibitory KIR-ligand incompatibility between donor and recipient in the GvH direction is KIR-ligand incompatibility and umbilical cord blood stem cell transplantation R Willemze et al associated with a decreased RI and an improved LFS and OS for patients with acute leukemia transplanted in CR with a singleunit unrelated umbilical cord blood graft. Multivariate analysis shows that these findings are independent of the number of HLA disparities between the donor and recipient. We confirm our and other previous studies showing that a higher number of HLA disparities (two or more) are associated with diminished neutrophil recovery, increased NRM rates and decreased RI. 19, 20 In contrast, KIR-ligand incompatibility was not associated with the cumulative incidence of neutrophil recovery within 60 days and NRM. A limitation of our study is the short median follow-up (13 months for the KIR-ligand-matched and 15 months for the KIRligand-mismatched donor-patient pairs) due to the fact that HLA-C allelic typing has just recently been used for both umbilical cords and patients. However, the aim of our study was not to assess the actual risk of relapse after UCBT but to compare the incidence of relapse after UCBT with or without KIR-ligand mismatches in the GvH direction. We cannot rule out that a KIR-ligand-mismatched combination delays relapse, which would become evident after a longer follow-up. Different effects of KIR/KIR-ligand incompatibilities on the outcome of allogeneic SCT have been reported. During the last 10 years, the understanding of NK-cell alloreactivity is rapidly expanding, and thus the methods, utilized through the years, to determine KIR mismatch, vary largely. Mismatches between donor KIR ligand and recipient KIR ligand in the GvH direction (the donor-recipient KIR-ligand model) have been used by the Perugia group and Hsu et al., [7] [8] [9] 12, 22 between donor KIR and recipient KIR by Gagne et al. 28 and between donor KIR and recipient KIR ligand by Leung et al. 29, 30 Criticizing the donor-recipient KIR-ligand model one could argue that, as some donors might not have possessed the relevant KIR gene, a minority of donor-recipient pairs might have been misclassified in this study. In fact, donor-versusrecipient NK-cell alloreactivity was predicted on the basis of KIR-ligand mismatching without the direct assessment of the donor alloreactive NK-clone repertoire or donor KIR genotyping. However, it is worth noting that KIR genotyping is required Table 3 Multivariate analysis for overall survival, leukemia-free survival, relapse incidence, non-relapse mortality and neutrophil recovery KIR-ligand incompatibility and umbilical cord blood stem cell transplantation in only a minority of donors as most possess a full complement of inhibitory KIR genes and have the potential to exert NK alloreactions. Moreover, the risk of misclassification arises when the donor does not possess/express the relevant KIR to exert alloreactivity. Under this circumstance, KIR-ligand mismatching mistakenly classifies a non-NK-alloreactive transplant pair as NK alloreactive. Therefore, in our series, such 'false positives' might have 'contaminated/diluted' the NK-alloreactive cohort. Thus, the only conceivable consequence of such misclassification is an underestimation of the effects of NK alloreactivity, which reinforces, rather than detracts from, our core observation that NK-cell alloreactivity favorably impacts on outcomes of cord blood transplantation. Our findings in UCBT are in agreement with those of the Perugia group, which, using haploidentical family donor SCT, has shown that KIR-ligand incompatibility in the GvH direction is associated with a decreased incidence of graft failure, GvHD and relapse, and an improved survival of patients with AML. 7-9 They explain their clinical results by findings from mouse experiments and human in vitro data in which donor-versus-recipient alloreactive NK cells eliminate residual leukemic cells, ablate host-type dendritic cells responsible for triggering GvHD and attack residual host lymphohematopoietic cells, including T cells, that are responsible for graft rejection. 8 Our present analyses show that KIR-ligand incompatibility has a stronger effect on RI, LFS and OS in AML patients than in ALL patients. This finding is in accordance with clinical and in vitro data from Ruggeri et al., 8 who showed that AML cells were killed in vitro by alloreactive NK clones, whereas only a sub-population of ALL cells was sensitive to NK-cell alloreactivity. As we observed a small but consistent KIR-ligand effect in our ALL patients, the data suggest that subgroups of ALL (T-or B-lineage ALL) may show differential sensitivities to donor NK-cell alloreactivity. This study provides for the first time information on the role of HLA-A3/-A11 KIR-ligand mismatches in transplantation, which had been lacking until now, as they were not reported in haploidentical transplants. Surprisingly, HLA-A3/-A11 mismatching in the GvH direction emerged as just as good a predictor of favorable prognosis as HLA-C and HLA-Bw4 group mismatches. Our use of HLA-A3/-A11 mismatching as a predictor of NK-cell alloreactivity may be criticized, as the role of HLA-A3/-A11 as a ligand for KIR3DL2 is controversial. As HLA-A3/-A11 alleles were reported to function as KIR ligands when bound to EBV peptides, 6 it seems reasonable that, in view of frequent EBV reactivation after transplantation, EBV peptides were readily available for HLA-A3/-A11 binding, with the possible generation of HLA-A3/-A11-specific donor NK cells that were alloreactive against target cells from recipients lacking HLA-A3 and/or HLA-A11. In our data set, EBV status was reported in only 74 patients (36%), of which 58 (78%) were EBV positive. No data on EBV reactivation are available in the Eurocord database. Thus, the role of HLA-A3/-A11 as a KIR ligand cannot be further supported in this study. This analysis shows that donor-versus-recipient NK-cell alloreactivity exerts a beneficial impact not only in haploidentical but also in cord blood transplantation. These two types of SCT have in common that the donors are highly HLA mismatched with the recipient, whereas after SCT, relatively little severe GvHD is observed. The post-transplant period is further characterized by a rapid recovery of NK cells and longlasting T-cell immunoincompetence. As T cells in the cord blood graft are antigen inexperienced, they contain few or no cytotoxic T cells directed against viral and bacterial peptides, which may cross-react with HLA alloantigens. The relative lack of post-thymic T cells and the T-cell naivety may be responsible for the lower-than expected frequency of severe GvHD, even in the setting of one or two HLA mismatches, and for the delayed recovery of T-cell function after UCBT [31] [32] [33] to the same degree as after haploidentical SCT. Lack of memory T cells in the graft (due to T-cell depletion in haploidentical and T-cell naivety in cord blood transplants) apparently permits the recovery of fully functional NK cells. Interestingly, one study comparing T celldepleted-versus-repleted unrelated donor transplants demonstrated that T cells in the graft adversely affected reconstitution of KIR-bearing NK cells and clinical outcomes. 34 Additional evidence that T cells antagonize reconstitution of potentially alloreactive, KIR-bearing NK cells derives from several other unrelated donor transplant studies, using T-cellreplete grafts. 10, 11, [13] [14] [15] 33, 35 Most studies showed no advantage in transplantation from KIR-ligand-mismatched donors, [13] [14] [15] 30, 31, [36] [37] [38] whereas a few observed an increased graft-versus-leukemia effect. 10, 11, [39] [40] [41] In haploidentical 29, 30 , matched sibling 12 and unrelated donor transplants, 36 the 'missing ligand' model was proposed as a powerful algorithm for predicting favorable transplant outcomes. It hypothesizes that alloreactions also occur when KIR-ligand-matched donors possess KIR(s) for which neither donor nor recipient has an HLA ligand(s). These donors were shown to carry KIR-bearing NK cells in an anergic/regulated state. 42 The 'missing ligand' model proposes that, upon transfer into the recipient, they may become activated and exert a graftversus-leukemia effect. However, in the UCBT setting, as in a recent haploidentical transplant study, 9 no informative results emerged when we analyzed the outcomes of 'missing ligand' transplants (data not shown). In conclusion, in patients with acute leukemia in CR, singleunit UCBT from donors who are KIR-ligand mismatched (for HLA-C group, HLA-Bw4 group and/or HLA-A3/-A11) in the GvH direction resulted in a lower incidence of relapse and in an improved LFS and OS as compared with UCBT from KIR-ligandcompatible donors. If these results are confirmed in a larger series of patients, KIR-ligand incompatibility in the GvH direction might be considered as a criterion for cord blood donor choice. 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A longterm clinicopathologic study of 20 Seattle patients Estimation of failure probabilities in the presence of competing risks: new representations of old estimators A class of K-sample tests for comparing the cumulative incidence of a competing risk A proportional hazards model for subdistribution of a competing risk Relevance of KIR gene polymorphisms in bone marrow transplantation outcome Comparison of killer Ig-like receptor genotyping and phenotyping for selection of allogeneic blood stem cell donors KIR-ligand incompatibility and umbilical cord blood stem cell transplantation R Willemze et al Determinants of antileukemia effects of allogeneic NK cells T-cell alloreactivity dominates natural killer cell alloreactivity in minimally T-cell-depleted HLA-non-identical paediatric bone marrow transplantation Similar potential to become activated and proliferate but differential kinetics and profiles of cytokine production of umbilical cord blood T cells and adult blood naive and memory T cells Immune reconstitution after unrelated cord blood transplantation KIR reconstitution is altered by T cells in the graft and correlates with clinical outcomes after unrelated donor transplantation Missing KIR ligands are associated with less relapse and increased graft-versus-host disease (GVHD) following unrelated donor allogeneic HCT KIR ligands and prediction of relapse after unrelated donor hematopoietic cell transplantation for hematologic malignancy Low number of donor activating killer immunoglobulinlike receptors (KIR) genes but not KIR-ligand mismatch prevents relapse and improves disease-free survival in leukemia patients after in vivo T-cell depleted unrelated stem cell transplantation Japan Marrow Donor Program. Donor killer immunoglobulin-like receptor (KIR) genotype-patient cognate KIR ligand combination and antithymocyte globulin preadministration are critical factors in outcome of HLA-C-KIR ligand-mismatched T cell-replete unrelated bone marrow transplantation Reduced risk for molecular disease in patients with chronic myeloid leukemia after transplantation from a KIRmismatched donor Comparison between antithymocyte globulin and alemtuzumab and the possible impact of KIR-ligand mismatch after dosereduced conditioning and unrelated stem cell transplantation in patients with multiple myeloma Successful use of haploidentical stem-cell transplantation with KIR mismatch as initial therapy for poor-risk myelodysplastic syndrome Human NK cell education by inhibitory receptors for MHC class I This study was supported by DRRIC (delegation de la recherche clinique), Université de Paris VII. RW, CAR, EG and VR designed the study, were the principal investigators and wrote the paper; ML was responsible for confirming the statistical analysis; II, KB, AH and FG were responsible for data collection and quality control of the Eurocord Database; GS, GM, GS, BR, AS, MR, LM, MM and JMR were each responsible for 45 cord blood stem cell transplantations in this study; FG, JG, LL, GK, YB, CN and MB represented the Cord Blood Banks that delivered each 45% of the cord blood units in this study. All authors read the paper, gave suggestions and approved its content. A complete list of the participating transplant centers and cord blood banks participating in this study can be found on the Leukemia website. Supplementary Information accompanies the paper on the Leukemia website (http://www.nature.com/leu) KIR-ligand incompatibility and umbilical cord blood stem cell transplantation