key: cord-0003515-0wcvoygd authors: Pahonțu, Elena; Ilieș, Diana-Carolina; Shova, Sergiu; Paraschivescu, Codruța; Badea, Mihaela; Gulea, Aurelian; Roșu, Tudor title: Synthesis, Characterization, Crystal Structure and Antimicrobial Activity of Copper(II) Complexes with the Schiff Base Derived from 2-Hydroxy-4-Methoxybenzaldehyde date: 2015-04-02 journal: Molecules DOI: 10.3390/molecules20045771 sha: 59ae1705d9fe9d34f1d7996bea46a83eacf92f47 doc_id: 3515 cord_uid: 0wcvoygd A novel Schiff base, ethyl 4-[(E)-(2-hydroxy-4-methoxyphenyl)methylene-amino]benzoate (HL), was prepared and structurally characterized on the basis of elemental analyses, (1)H NMR, (13)C NMR, UV-Vis and IR spectral data. Six new copper(II) complexes, [Cu(L)(NO(3))(H(2)O)(2)] (1), [Cu(L)(2)] (2), [Cu(L)(OAc)] (3), [Cu(2) (L)(2)Cl(2)(H(2)O)(4)] (4), [Cu(L)(ClO(4))(H(2)O)] (5) and [Cu(2)(L(2)S)(ClO(4))(H(2)O)]ClO(4)·H(2)O (6) have been synthesized. The characterization of the newly formed compounds was done by IR, UV-Vis, EPR, FAB mass spectroscopy, elemental and thermal analysis, magnetic susceptibility measurements and molar electric conductivity. The crystal structures of Schiff base and the complex [Cu(2)(L(2)S)(ClO(4))(H(2)O)]ClO(4)·H(2)O (6) have been determined by single crystal X-ray diffraction studies. Both copper atoms display a distorted octahedral coordination type [O(4)NS]. This coordination is ensured by three phenol oxygen, two of which being related to the µ-oxo-bridge, the nitrogen atoms of the azomethine group and the sulfur atoms that come from the polydentate ligand. The in vitro antimicrobial activity against Escherichia coli ATCC 25922, Salmonella enteritidis, Staphylococcus aureus ATCC 25923, Enterococcus and Candida albicans strains was studied and compared with that of free ligand. The complexes 1, 2, 5 showed a better antimicrobial activity than the Schiff base against the tested microorganisms. The synthesis of new compounds that are used for the treatment of infections with less secondary effects is a biomedical problem [1, 2] . Recently research has focused increasingly on the synthesis of transition metal complexes with Schiff-type ligands, due to the biological properties that they present. Many compounds derived from Schiff bases exhibit antibacterial, antifungal, antitumor and anti-HIV activities [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] . Schiff bases derived from salicylaldehyde represent an important class of ligands due to their ability to be used in various fields [15] [16] [17] [18] [19] . Having a high capacity chelating and redox potential the positive Cu 2+ ion is biologically active, and participates in many processes in the body [20] [21] [22] . Copper complexes are also among the most potent antiviral, antitumor and anti-inflammatory agents [23] . In addition, it was found that most of benzocaine-containing compounds are biologically active. Recent research has demonstrated that, these derivatives exhibit antimicrobial activity against different species [24] [25] [26] . Based on these observations and considering research in this area in the present study, we report the synthesis, characterization, antimicrobial studies of some copper(II) complexes containing a Schiff base ligand derived from 2-hydroxy-4-methoxybenzaldehyde and ethyl-4-aminobenzoate, with a special impetus on ligand structural investigations. The crystal structures of ligand and complex 6 were studied by X-ray diffraction. The biological properties of these compounds was tested for their antibacterial and antifungal activities against Escherichia coli ATCC 25922, Salmonella enteritidis, Staphylococcus aureus ATCC 25923 and Candida albicans strains. The Schiff base HL was prepared by refluxing in ethanol an equimolar mixture of 4-aminobenzoic acid ethyl ester with 2-hydroxy-4-methoxybenzaldehyde. The structure of the formed Schiff bases was established by IR, 1 H-NMR and 13 C-NMR spectroscopies and by X-ray crystallography. All compounds were synthesized by direct reaction between ligand and the corresponding metal salts, while compound 6 was prepared by using a mixture of Cu(ClO4) 2·6H2O and HL with addition of NaSCN. All complexes are microcrystalline solids. Also, the melting point values are greater than 220 °C. They are insoluble in organic solvents such as acetone and methanol, but soluble in DMF and DMSO. The molar conductivity values (8-12 ohm −1 ·cm 2 ·moL −1 ) of the complexes 1-5 in 10 −3 M solution DMF show that they are non-electrolytes and 6 (82 ohm −1 ·cm 2 ·moL −1 ) is an electrolyte [27] . The thermal decompositions of the complexes 1-4 were studied by thermogravimetry (TG). The TG and TGD curves have three or four stages of mass loss. The first weight loss step correspond of two and four water molecules respectively, per one molecule of complex (7.26% for complex 1, 8.63% for complex 4). Thermal analyses data reveal that compounds 2 and 3 not are hydratated. The final residue was analyzed by IR spectroscopy, which confirms the formation of CuO ( Figure S1 ). The elemental analyses data of the Schiff base and complexes (reported in Section 3) are in agreement with structure of the ligand ( Figure 1 ) and with structures of the complexes (Figure 2 ). (1) (3) (4) The ligand HL structure is shown in Figure 3 . The planar configuration of HL molecule is stabilized by an intra-molecular O-H⋅⋅⋅N hydrogen bond which is observed in the majority of Schiff base ligands obtained from 2-hydroxybenzaldehyde [28] . The crystal packing of HL is stabilized by π-π staking and C-H···O cooperative interactions. Cg1 (the centroid of the C-C9 ring) exhibits a stacking interaction with Cg2 (the centroid of the C11-C16 ring) of the adjacent molecule, related by two-fold axis, with a centroid-to-centroid distance of 3.851(4) Å. The formed dimeric units ( Figure 4 ) are further associated due to the presence of weak C-H···O intermolecular interactions resulting into the formation of two-dimensional supramolecular ribbons, as the main crystal structure packing motif, depicted in Figure 5 . Tables 1 and 2 . Complex 6 has a crystal structure formed from the complex binuclear cations [Cu2(L2S)2(ClO4)(H2O)] + , ClO4 − anions and H2O as solvate molecules 1:1:1 molar ratio. The structure of the complex cation is presented in Figure 6 . In the complex cation two Cu 2+ ions, separated by 3.029(1) Å, are coordinated by two mono-deprotonated HL − ligands, so that the charge balance is in agreement with the formation of species [Cu2(L2S)2ClO4H2O] + . The separate structure of the coordinated ligands along with the atomic labeling scheme is presented in Figure 7a ,b. Both the ligands are identical from the chemical point of view and exhibit very close geometric parameters ( Table 2 ). The localization of the hydrogen atoms using the difference density Fourier map has clearly shown that the non-coordinated azomethine atoms N1 and N4 are the sites of protonation associated with the hydrogen bonding towards phenolic oxygen atoms as acceptor (Figures 6 and 7) . The copper atoms adopt a slightly distorted octahedral [O4NS] coordination completed by ClO4 − anion and H2O molecule as monodentate ligands for Cu1 and Cu2, respectively ( Figure 8 ). The crystal structure of complex 6 is characterized by the parallel packing of two dimensional supramolecular layers consolidated due to the numerous O-H···O and C-H···O hydrogen bonding, as shown in Figure 9 . The ligand and complexes have been characterized in detail by recording their IR spectra. The proposed assignments are based on previous results [28] [29] [30] [31] and pertinent bibliography [32] [33] [34] [35] . The ʋ(C=N) band of the ligand at 1622 cm −1 is found to be shifted to lower energies (1603-1595 cm −1 ) in the spectra of the complexes, indicating coordination via the azomethine nitrogen. The phenolic ʋ(C-O) stretching vibration in the free Schiff base is observed at 1113 cm −1 , which is shifted by 10-21 cm −1 towards lower wave numbers in the complexes, thus indicating coordination of the phenolic oxygen to the Cu 2+ ion [36] . In the IR spectra of complexes 1, 4 and 5, a considerable peak observed in the 3467-3420 cm −1 range supports the presence of ʋ(H2O) in the complexes [37] . The nitrate complex 1 has two bands at 1433 and 1182 cm −1 corresponding to ʋ5 and ʋ1, with a separation of 250 cm −1 , and a medium band at 930 cm −1 assigned to ʋ2 of the nitrato group, values that indicate bidentate coordination [38, 39] . The acetate complex 3 has two strong bands at 1530 and 1442 cm −1 corresponding to ʋas(COO − ) and ʋs(COO − ) with a difference between frequencies of 88 cm −1 . This difference confirms the bidentate nature of the coordinated acetate [40] . The perchlorate complex 5 shows a band at 1108 cm −1 assignable to ʋ3(ClO4 − ) and a strong band at 1090 cm −1 assignable to ʋ4(ClO4 − ). The splitting of this band in two components indicates the presence of a monodentate perchlorate group [33, 38] . The tentative assignments of the significant electronic spectral bands of ligand and of complexes are presented in Table 3 . In these spectra, there is an intense band at 33,330 cm −1 which is assigned to a π→π* transition originating in the phenyl ring [41] . The band at 28,570 cm −1 is attributed to an n→π* transitions originating in the -CH=N-chromophore [42] . In the spectra of the complexes these bands are shifted to lower energies ( Figure S2 ). The electronic spectra for the Cu(II) complexes 2, 3 and 5 exhibit two bands in the region 10,980-11,100 cm −1 respectively 13,150-13,880 cm −1 , attributed to transitions d-d: 2 B2→ 2 E, 2 B2→ 2 B1( 2 A1), suggesting a distorted tetrahedral geometry [43, 44] . The values of the magnetic momentum (1.87, 1.92 and 1.90 BM) indicate the existence of copper complexes under monomers form. The spectrum for complex 1 exhibit three bands at 10,500, 13,420 and 19,410 cm −1 corresponding to the transitions: 2 B1g→ 2 A1g 2 , 2 B1g→ 2 B2g, 2 B1g→ 2 Eg, specific to the octahedral complexes with ground state [43] . The magnetic moment value 1.98 BM for complex 1 indicative of a non-bridging copper(II) complex. The electronic spectrum of complex 4 exhibit three bands at 11,000, 12,110 and 16,940 cm −1 corresponding to the transitions: 2 B1g→ 2 A1g, 2 B1g→ 2 B2g, 2 B1g→ 2 Eg suggesting a octahedral geometry [43] . The magnetic moment of the complex 4, measured at room temperature, is 1.07 BM, per copper center. The subnormal magnetic moment indicates that the copper centers are anti-ferromagnetically coupled [45] [46] [47] [48] [49] [50] [51] [52] [53] [54] . The FAB mass spectra of Cu(II) complexes with base Schiff HL have been recorded (Table S1 ). The mass spectrum of ligand showing a peak at m/z = 300.12 corresponds to the molecular ion [M] + ( Figure S3 ). [M] + peak obtained from the complexes are as follow: m/z = 386.9 (1), m/z = 658.0 (2), m/z = 399.1 (3), m/z = 759.0 (4), m/z = 361.0 (5). The results of mass spectrometry are consistent with the proposed formulas for these compounds as the peaks observed in these spectra correspond to fragments resulting from the expected fragmentations of the compounds. The EPR spectra of the complexes in the polycrystalline state at 298 K and 77 K, were recorded at X-band, using 100 kHz field modulation. The g factors were quoted relative to the standard marker TCNE. The spectra of the compounds 1 and 4 in the polycrystalline state (293 K) show typical axial behavior with different gǁ and g ⊥ values ( Table 4 ). The geometric parameter G which is a measure of the exchange interaction between the copper centers in the polycrystalline compound is calculated using the equation: G = (gǁ − 2.0023)/(g ⊥ − 2.0023), for axial spectra [55] . In the copper(II) complexes 1 and 4, gǁ > g ⊥ > 2.0023 and G values (2.77, 3.99) are consistent with a ground state [56] . The spectra of compounds 2, 3 and 5 show only one broad signal at 2.097, 2.102 and 2.127, respectively, ( Figure 10 ) consistent with tetrahedral geometry [57] . Magnetic field (G) 2 Figure 10 . EPR spectra for the complexes 2, 3 and 5 in powder, at room temperature. The solution of complexes were recorded in DMSO at 298 K and 77 K. They present well-resolved four hyperfine lines ( Figure 11 ). EPR spectral assignments of the copper (II) complexes and orbital reduction parameters are shown in Table 4 . The EPR parameters gǁ, g ⊥ , Aǁ (Cu) and the energies of the d-d transition were used to evaluate the bonding parameters α 2 , β 2 and δ 2 , which may be regarded as measures of the covalency of the in-plane σ bonds, in-plane π bonds and out-of-plane π bonds [58, 59] . The orbital reduction factors Kǁ = α 2 β 2 and K ⊥ = α 2 δ 2 , were calculated using expressions reported elsewhere [60] . Hathaway has pointed out that for pure σ bonding, Kǁ ~ K ⊥ ~ 0.77 and for in-plane π -bonding, Kǁ < K ⊥ , while for out-of-plane π-bonding, Kǁ > K ⊥ [61] . The synthesized compounds and ligand were tested for their in vitro antibacterial and antifungal activity against Escherichia coli ATCC 25922, Salmonella enteritidis, Staphylococcus aureus ATCC 25923, Enterococcus and Candida albicans strains using the paper disc diffusion method [62] (for the qualitative determination) and the serial dilutions in liquid broth method [63] for determination of MIC values. Furaciline and nistatine were used as reference substances. Data from Table 5 confirm the fact that both the ligand HL and the coordinative complexes of copper have a reduced bacteriostatic activity within the limits of concentrations 0.5-10.0 mg/mL toward the gram-positive and gram-negative bacteria. A more nuanced sensitivity toward the searched complexes has been proved by the gram-positive micro-organisms. The experimental data obtained demonstrate that the bacteriostatic activity of the coordinative complexes is influenced by the number of ligand molecules HL found out in the inner sphere and ligand nature. Therefore, growing of the number of ligand molecules and replacing the ions chloro-or acetate-with the perchlorate ion leads to a growing of the antimicrobial activity. All commercially available reagents and chemicals were of analytical-or reagent-grade purity and used as received. The chemical elemental analysis for the determination of C, H, N was done on a Carlo-Erba LA-118 microdosimeter (Lakewood, CA, USA). 1 H-NMR and 13 C-NMR spectra were recorded at room temperature on a Bruker DRX 400 spectrometer (Billerica, MA, USA) in DMSO-d6, using TMS as the internal standard. IR spectra were recorded on a Specord-M80 spectrophotometer (Leipzig, Germany) in the 4000-400 cm −1 region using KBr pellets. The complexes were studied by thermogravimetry (TG) in a static air atmosphere, with a sample heating rate of 10 °C/min using a STA 6000 Perkin Elmer (Waltham, MA, USA). Electronic spectra were recorded using the JascoV-670 spectrophotometer (Tokyo, Japan) in diffuse reflectance, using MgO dilution matrices. EPR spectra were recorded on polycrystalline powders and DMSO solutions at room temperature and 77 K with an MiniScope MS200, (Magnettech Ltd., Berlin, Germany), X-band spectrometer (9.3-9.6 GHz), connected to a PC equipped with a 100 KHz field modulation unit. High resolution mass spectra were recorded on a ThermoScientific (LTQ XLOrbitrap) spectrometer using APCI ionization technique and Orbital Ion Trap mass analyzer (Rockford, IL, USA). The molar conductance of the complexes in N,N'-dimethylforamide solutions (10 −3 M), at room temperature, were measured using a Consort type C-533 conductivity instrument. The magnetic susceptibility measurements were done at room temperature in the polycrystalline state on a Faraday magnetic balance (home made). Crystallographic measurements were carried out on Oxford-Diffraction XCALIBUR E CCD diffractometer (Santa Clara, CA, USA) equipped with graphite-monochromated MoKα radiation. The single crystals were positioned at 40 mm from the detector and 197 and 273 frames were measured each for 20 and 30 s over 1° scan width for HL and 6, respectively. The unit cell determination and data integration were carried out using the CrysAlis package of Oxford Diffraction [64] . Both structures were solved by direct methods using Olex2 [65] software with the SHELXS structure solution program and refined by full-matrix least-squares on F² with SHELXL-97 [66] . Atomic displacements for non-hydrogen atoms were refined using an anisotropic model. H atoms were placed at calculated positions and refined as riding atoms in the subsequent least-squares model refinements. The positional parameters of OH and NH hydrogen atoms were found from difference. The oxygen atoms in non-coordinated perchlorate anion were found to be severely disordered and their positional parameters were refined isotopically in combination with PART and SADI tools available in SHELXL Crystallographic data together with refinement details are summarized in Table 6 . where n is the number of reflections and p is the total number of parameters refined. A solution of 2-hydroxy-4-methoxybenzaldehyde (0.152 g, 1 mmoL) in ethanol (10 mL) was added to a solution of ethyl-4-aminobenzoate (0.165 g, 1 mmoL) in ethanol (20 mL). The mixture was stirred for 1 h at 25 °C, then refluxed for 4 h and kept at 4 °C for several days. The characteristic yellow precipitate obtained by Schiff base condensation was filtered out and kept for crystallization. Fine yellow crystals obtained upon slow evaporation at room temperature were characterized, including single crystal X-ray diffraction. The antibacterial activity of complexes and also of their prototype furaciline has been determined under liquid nutritive environment [2% of peptone bullion (pH 7.0)] using successive dilutions method [67] [68] [69] . Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Enterococcus stems were used as reference culture for in vitro experiment. The dissolution of studied substances in dimethylformamide, microorganisms cultivation, suspension obtaining, determination of minimal inhibition concentration (MIC) and minimal bactericide concentration (MBC) have been carried out according to the method previously reported. Antimycotic properties of the complexes were investigated in vitro on laboratory stem Candida albicans. The activity has been determined in liquid Sabouroud nutritive environment (pH 6.8). The inoculates were prepared from fungal stems which were harvested from 3-7 day-old cultures. Their concentration in suspension was (2-4) × 10 6 colony forming units per milliliter. Sowings for fungi and micelles were incubated at 37 °C during 7 and 14 days, respectively. The analytical and physico-chemical analyses confirm the composition and the structure of the newly obtained complex combinations. In all the complexes, the ligand base Schiff, HL acts as mono-negative bidentate around the metal ion. The structure of the ligand HL and complex 6 has been determined by single-crystal X-ray diffraction. This fact indicates for complex 6 a bi-nuclear system where every atom of Cu(II) is hexacoordinated through the medium of 4 atoms of oxygen, one atom of nitrogen and one atom of sulfur. The two atoms of copper (II) are united each other through the medium of two atoms of oxygen coming from the hydroxyl groups grafted on the aldehyde nucleus. The EPR parameters gǁ, g ⊥ , Aǁ and the energies of d-d transitions were used to evaluate the bonding parameters. The orbital reduction factors indicate the presence of out-of-plane π-bonding for complexes 1, 3-5 and of some in-plane π-bonding for the complex 2. The antimicrobial activity data obtained indicate that the activity of the metal complexes is higher than ligands. The structure of the compounds tested has an effect on antimicrobial activity. Supplementary materials can be accessed at: http://www.mdpi.com/1420-3049/20/04/5771/s1. Design, synthesis, and evaluation of 2-aryl-3-heteroaryl-1, 3-thiazolidin-4-ones as anti-HIV agents Synthesis of functionalized arylaziridines as potential antimicrobial agents Design, synthesis, testing and quantitative structure-activity relationship analysis of substituted salicylaldehyde Schiff bases of 1-amino-3hydroxyguanidine tosylate as new antiviral agents against coronavirus Synthesis of novel azo Schiff bases and their antibacterial and antifungal activities Spectroscopic characterization of metal complexes of novel Schiff base. Synthesis, thermal and biological activity studies Antioxidant, electrochemical, thermal, antimicrobial and alkane oxidation properties of tridentate Schiff base ligands and their metal complexes Rashkov, I. Preparation, characterization and biological activity of Schiff base compounds derived from 8-hydroxyquinoline-2-carboxaldehyde and Jeffamines ED Some ferrocenyl Schiff bases with nonlinear optical properties Synthesis of 3-acetoxyazetidin-2-ones and 3-hydroxyazetidin-2-ones with antifugal and antifungal and antibacterial activity Synthesis, crystal structure, antioxidation and DNA binding properties of binuclear Ho(III) complexes of Schiff-base ligands derived from 8-hydroxyquinoline-2-carboxyaldehyde and four aroylhydrazines Oxidative cleavage of DNA by a dipyridoquinoxaline copper(II) complex in the presence of ascorbic acid Synthesis, spectroscopic, structural characterization, electrochemical and antimicrobial activity studies of the Schiff base ligand and its transition metal complexes Ni(II) and Cu(II) complexes with thiosemicarbazone (L1) and semicarbazone (L2) derived from pyrole-2-carboxyaldehyde Inhibition of herpes simplex virus type 1 and adenovirus type 5 by heterocyclic Schiff bases of aminohydroxyguanidine tosylate Ru(II) complexes of N4 and N2O2 macrocyclic Schiff base ligands: Their antibacterial and antifungal studies Chiral Ru(II) Schiff base complexes catalysed enantioselective eposidation of styrene derivatives using iodosyl benzene as oxidant II Catalytic reactions of metalloporphyrins. 1. Catalytic modification of borane reduction of ketone with rhodium(III) porphyrin as catalyst A rationally designed, chiral Lewis acid for the asymmetric induction of some Diels-Alder reactions A new route for the synthesis of bis(pyridine dicarboxylato)bis(triphenylphosphine) complexes of ruthenium(II) and X-ray structural characterisation of the biologically active trans Cu(II), Ni(II) and Zn(II) Metals: Synthesis, Spectroscopic and Medicinal Studies Mitochondria and apoptosis Synthesis, cytotoxicity, and antitumor activity of copper(II) and iron(II) complexes of (4)N-azabicyclo[3.2.2]nonane thiosemicarbazones derived from acyl diazines Applications of metal complexes of Schiff bases-A review Antimicrobial properties of topical anesthetic liquids containing lidocaine or benzocaine Über neue pharmakologisch wirksame Amide und Ester der Nicotinsäure Purification of Laboratory Chemicals The use of conductivity measurements in organic solvents for the characterization of coordination compounds Some new Cu(II) complexes containing an ON donor Schiff base: Synthesis, characterization and antibacterial activity Copper(II) Complexes with Ligands Derived from 4-Amino-2,3-dimethyl-1-phenyl-3-pyrazoline-5-one Complexes of 3d(n) metal ions with thiosemicarbazones: Synthesis and antimicrobial activity Metal-based biologically active agents: Synthesis, characterization, antibacterial and antileukemia activity evaluation of Cu(II), V(IV) and Ni(II) complexes with antipyrine-derived compounds Mn(II) with Schiff base derived from 4-amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one Copper(II) complexes of N(4)-substituted thiosemicarbazones derived from pyridine-2-carbaldehyde: Crystal structure of a binuclear complex Synthesis, X-ray crystal structure, and solution properties of nickel(II) complexes of new 16-membered mixed-donor macrocyclic Schiff base ligand incorporating a pendant alcohol function Metal complexes with a new N4O3 amine pendant-armed macrocyclic ligand: Synthesis, characterization, crystal structures, and fluorescence studies Coordination chemistry of copper(II) with oxaaza macrocyclic ligands: Crystal structure of a dinuclear tetramer copper(II) complex Low-spin iron(III) Schiff-base complexes with symmetric hexadentate ligands: Synthesis, crystal structure, spectroscopic and magnetic properties Synthesis, structure and properties of some copper(II) complexes containing an ONO donor Schiff base and substituted imidazole ligands Infrared Spectra of Inorganic and Coordination Compounds Infrared and Raman Spectra of Inorganic and Coordination Compounds Bis(triphenylphosphine)4-fluorobenzaldehyde thiosemicarbazone copper(I): Forcing chelation through oxoanions An interpretation of the circular dichroism and electronic spectra of salicylaldimine complexes of square-coplanar diamagnetic nickel(II) Circular dichroism of square-planar, tetradentate Schiff base chelates of copper(II) Inorganic Electronic Spectroscopy New copper(II) complexes of 2-hydroxyacetophenone N(4)-substituted thiosemicarbazones and polypyridyl co-ligands: Structural, electrochemical and antimicrobial studies Comprehensive Coordination Chemistry Transition Metal Chemistry Syntheses, structure, and electrochemical characterization of homo-and heterodinuclear copper complexes with compartmental ligands From mononuclear to polynuclear macrocyclic or macroacyclic complexes Syntheses and magnetic properties of heterobinuclear complexes with N,N'-bis(3-caboxylsalicylidene)trimethylenediamine Synthesis of novel dinickel(II) and nickel(II)-copper(II) bimetallic complexes derived from an acyclic dinucleating Schiff base-pyridine ligand Binuclear copper(II) complexes of a new sulphur-containing binucleating ligand: Structural and physicochemical properties Physical Inorganic Chemistry Approach Theory of Superexchange Interaction The electronic properties and stereochemistry of mononuclear complexes of the copper(II) ion Electronic properties and stereochemistry of the copper(II) ion. Part VII. Mono(diethylenetriamine)copper(II) complexes Electronic bonding of Cu 2+ in amorphous and crystalline TeO2: EPR and optical spectra Electron spin resonance in transition metal chelates. I. Copper (II) bis-acetylacetonate ESR studies on the bonding in copper complexes Structure and Bonding Comprehensive Coordination Chemistry Procedures and theoretical considerations for testing antimicrobial agents in agar media NCCLS: Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria, Approved Guideline; Document M45-A, 26(19) Version 1.171.36.32; Oxford Diffraction Ltd Olex2: A complete structure solution, refinement and analysis program A short history of SHELXS -oxo-1-naftali)-N1-αoxobenzalihidrazincopper] Dihidrate Synthesis and antimicrobial activity of the complexes of 3d-metals with substituted salicylaldehyde benzoylhydrazones Synthesis and antimicrobial activity of sulfanylamide-containing copper(II) and nickel(II) salicylidene thiosemicarbazidates This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license The authors thank the Organic Chemistry Department, ICECHIM Bucharest (Romania) for microanalysis, "Horia Hulubei" National Institute of Physics and Nuclear Engineering Bucharest for help with EPR spectroscopy. The authors wish to thank the State University of Medicine and Pharmacy of Chisinau CCDC 1005531 and 1005532 contains the supplementary crystallographic data for C17H17NO4 (HL) and C68H66Cl2Cu2N4O26S2 (6) . These data can be obtained free of charge via http://www.ccdc.cam.ac.uk/data_request/cif or from the Cambridge Crystallographic Data Centre, 12 Union Road, Cambridge CB2 1EZ, UK; Fax: +44-1223-336-033; or E-Mail: deposit@ccdc.cam.ac.uk. Elena Pahontu designed the research, performed the synthesis of the compounds, contributed to the analysis of the data and wrote the paper. Diana-Carolina Ilieș performed IR spectroscopy, thermal analysis and wrote the paper; Sergiu Shova performed X-ray diffraction studies; Codruța Paraschivescu performed FAB mass spectroscopy; Mihaela Badea performed UV-Vis spectroscopy; Aurelian Gulea performed antimicrobial activity; Tudor Roșu performed EPR spectroscopy and contributed to the interpretation of the data.All authors read and approved the final manuscript. The authors declare no conflict of interest.