key: cord-0003492-92kyi7nn authors: Megahed, Mohamed E.; Cruz-Flores, Roberto; Dhar, Arun K. title: Complete Genome Sequences of Four Major Viruses Infecting Marine Shrimp in Egypt date: 2018-09-06 journal: Microbiol Resour Announc DOI: 10.1128/mra.00809-18 sha: b4977e3f26125c15ddb66fbfc8515e08ad166bf0 doc_id: 3492 cord_uid: 92kyi7nn The genome sequences of four economically important shrimp viruses, Penaeus stylirostris densovirus 1, hepatopancreatic parvovirus, yellow head virus, and gill-associated virus, are reported here. Genome data are fundamental for epidemiological studies in determining the origins of these viruses detected for the first time in Egypt and in developing disease management strategies. P enaeus semisulcatus and Marsupenaeus japonicas are the two native shrimp species in Egypt cultured since the 1980s. With the introduction of Fenneropenaeus indicus, a nonnative species, shrimp farmers started experiencing disease outbreaks. Laboratory diagnosis revealed the presence of Penaeus stylirostris densovirus 1 (PstDNV1), hepatopancreatic parvovirus (HPV), yellow head virus (YHV), and gill-associated virus (GAV). The genome sequences of these viruses are presented here. Total DNA and RNA were extracted from pleopods and hepatopancreas of shrimp using DNeasy and RNeasy minikits (Qiagen). Samples that tested positive by PCR (PstDNV1 and HPV) and reverse transcription-PCR (RT-PCR) (YHV and GAV) were taken for cloning. DNA and cDNA were digested with restriction enzymes, and plasmid libraries were made before taking recombinant clones for shotgun sequencing using an ABI 3730XLS sequencer (Macrogen, Inc.). Contigs were generated and aligned to the viral reference genomes to separate viral contigs from shrimp genome contigs. Sequences were examined using Chromas version 1.45 (1) and BioEdit 7.0.9, open reading frames (ORFs) were annotated using BLASTn, and Geneious R11 and multiple alignments were performed using CLUSTALW. PstDNV1 and HPV are classified in the family Parvoviridae, genus Brevidensovirus (2). Altogether, 11 PstDNV1 contigs 3,530 to 3,912 nucleotides (nt) long were generated. Each sequence contained ORFs encoding nonstructural (NS) proteins NS1 and NS2 and coat protein, as reported for other PstDNV1 isolates (3, 4) . A pairwise comparison of PstDNV1-Egypt isolates to the reference strain (GenBank accession number AF218266) (5) showed 92.33% to 100% identity. The genome sizes of 10 HPV-Egypt isolates varied from 5,685 to 6,336 nt. All but one isolate (GenBank accession number KT316245) contained three ORFs in the order of NS2, NS1, and VP, similar to the reference strain (6) . HPV-Egypt isolates showed 80.62% to 100% identity to the reference sequence (RefSeq accession number NC_007218) (6). The KT316245 isolate presented left (partial), middle, and right ORFs and showed 100% identity to an isolate from Madagascar (7). The YHV and GAV are bacilliform, enveloped, positive, single-stranded RNA (ssRNA) viruses in the family Roniviridae, genus Okavirus (8) . The genome of YHV-Egypt was 26,662 nt containing 3 ORFs (9). ORF1 encodes a polyprotein containing a 3C-like protease (nt 8571 to 9422) domain and a ribosomal frameshift slippery motif (AAAUUUU) (nt 12254 to 12260) that joins ORF1 and ORF2 (10). ORF2 encodes a p20 nucleocapsid protein, a helicase domain (nt 16688 to 17534), and an RNA-dependent DNA polymerase motif (nt 14291 to 15046). ORF3 (nt 20939 to 25984) encodes a polyprotein that generates two envelope glycoproteins (9, 11) . YHV-Egypt showed 100% identity to an isolate from Thailand (GenBank accession number FJ848674). The genome of GAV-Egypt was 26,253 nt, containing 4 ORFs (12) . ORF1a contains a 3C-like protease (nt 8535 to 9386) domain and the ribosomal frameshift slippery motif AAAUUUU (nt 12215 to 12221) (13, 14) . ORF1b contains an RNA-dependent DNA polymerase motif (nt 14252 to 15007) and a helicase domain (nt 16136 to 17515) (15) . ORF2 encodes p20 nucleocapsid protein (nt 20183 to 20617), and ORF3 contains a polyprotein (nt 20630 to 25597) of unknown function (12, 16) . GAV-Egypt showed 100% identity to an Australian isolate (GenBank accession number AF227196) (13) . The genome data are valuable in determining the origins of these viruses in Egypt. Data availability. We thank the shrimp farmers at DTZ, Egypt, for providing shrimp samples and information regarding the history of the disease outbreaks. Special thanks are due to the fishermen who cooperated and supplied wild shrimp samples from different sites. This work was supported by Science & Technology Development Fund grants 5661 and 185840. Partial funding for this research was provided by The University of Arizona Aquaculture Pathology Laboratory Diagnostic Fund and by the College of Agriculture & Life Sciences to Arun K. Dhar. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Department of Microbiology The family Parvoviridae Infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito brevidensoviruses Biology, genome organization, and evolution of parvoviruses in marine shrimp Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps Complete nucleotide sequence and genomic organization of hepatopancreatic parvovirus (HPV) of Penaeus monodon Nucleotide sequence of a Madagascar hepatopancreatic parvovirus (HPV) and comparison of genetic variation among geographic isolates Homologous genetic recombination in the yellow head complex of nidoviruses infecting Penaeus monodon shrimp Complete genome sequence of an isolate of a novel genotype of yellow head virus from Fenneropenaeus chinensis indigenous in China RNA transcription analysis and completion of the genome sequence of yellow head nidovirus Identification and analysis of gp116 and gp64 structural glycoproteins of yellow head nidovirus of Penaeus monodon shrimp The complete genome sequence of gillassociated virus of Penaeus monodon prawns indicates a gene organisation unique among nidoviruses Gill-associated virus of Penaeus monodon prawns: an invertebrate virus with ORF1a and ORF1b genes related to arteri-and coronaviruses The 3C-like proteinase of an invertebrate nidovirus links coronavirus and potyvirus homologs Complete ORF1b-gene sequence indicates yellow head virus is an invertebrate nidovirus The gene encoding the nucleocapsid protein of gill-associated nidovirus of Penaeus monodon prawns is located upstream of the glycoprotein gene