key: cord-0002100-60y9to0r
authors: Kotani, Osamu; Naeem, Asif; Suzuki, Tadaki; Iwata-Yoshikawa, Naoko; Sato, Yuko; Nakajima, Noriko; Hosomi, Takushi; Tsukagoshi, Hiroyuki; Kozawa, Kunihisa; Hasegawa, Hideki; Taguchi, Fumihiro; Shimizu, Hiroyuki; Nagata, Noriyo
title: Neuropathogenicity of Two Saffold Virus Type 3 Isolates in Mouse Models
date: 2016-02-01
journal: PLoS One
DOI: 10.1371/journal.pone.0148184
sha: d18e10321c54248f5fdfdac2501f5368ac8052c7
doc_id: 2100
cord_uid: 60y9to0r

OBJECTIVE: Saffold virus (SAFV), a picornavirus, is occasionally detected in children with acute flaccid paralysis, meningitis, and cerebellitis; however, the neuropathogenicity of SAFV remains undetermined. METHODS: The virulence of two clinical isolates of SAFV type 3 (SAFV-3) obtained from a patient with aseptic meningitis (AM strain) and acute upper respiratory inflammation (UR strain) was analyzed in neonatal and young mice utilizing virological, pathological, and immunological methods. RESULTS: The polyproteins of the strains differed in eight amino acids. Both clinical isolates were infective, exhibited neurotropism, and were mildly neurovirulent in neonatal ddY mice. Both strains pathologically infected neural progenitor cells and glial cells, but not large neurons, with the UR strain also infecting epithelial cells. UR infection resulted in longer inflammation in the brain and spinal cord because of demyelination, while the AM strain showed more infectivity in the cerebellum in neonatal ddY mice. Additionally, young BALB/c mice seroconverted following mucosal inoculation with the UR, but not the AM, strain. CONCLUSIONS: Both SAFV-3 isolates had neurotropism and mild neurovirulence but showed different cell tropisms in both neonatal and young mouse models. This animal model has the potential to recapitulate the potential neuropathogenicity of SAFV-3.

Saffold virus (SAFV) is a cardiovirus belonging to the family Picornaviridae. In 2007, SAFV was isolated from a stool sample obtained from an infant who suffered a fever of unknown origin in 1981 [1] . Prior to this, cardioviruses were thought to cause serious diseases, such as myocarditis, encephalomyelitis, and diabetes, mainly in small rodents [2] [3] [4] [5] [6] . The zoonotic Encephalomyocarditis virus (which belongs to the genus Cardiovirus) is infectious in primates, including humans, but causes only occasional and mild outbreaks [7] [8] [9] [10] [11] [12] . After its initial identification, SAFV was primarily detected in fecal and throat swab specimens from infants and children with acute gastroenteritis or acute upper respiratory symptoms [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] . Epidemiological and genetic analyses suggest that SAFV, especially genotypes 2 and 3, is circulating freely in the human population and is transmitted from human to human during early childhood because the sera of children and the elderly have high rates of neutralizing antibodies against SAFV genotypes 2 and 3 [18, 19, 22, 24, 26, 27] . The prevalence of SAFV in other mammals, including rodents, is still uncertain.

The pathogenicity of SAFV remains unclear because the virus is often detected along with enteric or respiratory viruses such as norovirus, rotavirus, bocavirus, and influenza virus [13, 14, 16, 17, 20, 21, 23, 25, [28] [29] [30] [31] , and even in healthy individuals [15] ; thus it is difficult to attribute infections to a specific virus. On the other hand, SAFV mono-infection was found in children with diarrhea, hand-foot-mouth disease, and upper respiratory inflammation [25, [30] [31] [32] . In addition, a surveillance program for acute flaccid paralysis (for global polio eradication) conducted by the World Health Organization occasionally detected SAFV in fecal specimens from patients with non-polio acute flaccid paralysis [15, 27, 33, 34] . Although SAFV is also found in the cerebrospinal fluid (CSF) of infants with cerebellitis [35] and aseptic meningitis [36] , it is rarely observed in CSF samples from those with neurological diseases [13, 30, 35] ; thus the role of SAFV in neurological diseases remains uncertain.

Enteroviruses belonging to the family Picornaviridae, especially coxsackieviruses A and B, preferentially infect neonatal mice rather than adult mice [37] . Thus, neonatal mice have been used for viral isolation and to investigate the pathogenesis of enteroviruses. However, picornaviruses generally show high host specificity; the natural host for all human enteroviruses is the human [37] . Enterovirus 71, a member of the family Picornaviridae, can cause meningitis and encephalitis because it infects human neurons. Some human isolates induce paralysis in neonatal mice after experimental infection of the brain. However, the mice often show severe myositis due to viral infection of not only neurons but also skeletal muscle; this does not happen in humans [38] . By contrast, coxsackievirus B and mouse-adapted enterovirus 71 infect large neurons in the brains of neonatal or adult mice and these animal models mimic the neuropathology observed in the human brain [39] [40] [41] [42] .

Therefore, establishing a mouse model of SAFV infection would advance understanding of the neuropathogenesis of SAFV. Indeed, a recent report [43] shows that mice subjected to intracerebral inoculation with HeLa cell-adapted SAFV type 2, which had been passaged eight times in rhesus monkey kidney epithelial (LLC-MK 2 ) cells and then 13 times in the alpha/beta interferon-deficient human glial cell line, U118MG, developed neurological disorders. In the present study, the primary aim was to examine the susceptibility of mouse brain to SAFV-3 in the absence of viral host adaptation. We used two clinical isolates of SAFV-3: the JPN 08-404 strain (isolated from the CSF of a patient with aseptic meningitis) [36] , and the Gunma/176/ 2008 strain (isolated from a throat swab from a patient with acute upper respiratory inflammation) [21] . Neonatal and young ddY mice, and young BALB/c mice were infected and analyzed using virological, pathological, and immunological methods. The results showed that both clinical isolates of SAFV-3 infected neural progenitor cells and glial cells, but not large neurons, and that both were mildly neurovirulent in mice; however, the clinical isolates showed different cell tropism and neurovirulence. Taken together, these results suggest that SAFV-3 is a candidate neuropathogenic virus that causes aseptic meningitis and other neurological disorders.

Two clinical isolates of SAFV-3 were used in this study: JPN 08-404, obtained from the CSF of a patient with aseptic meningitis in 2008 (referred to herein as the AM strain) [36] , and Gunma/176/2008, acquired from a throat swab from a patient with upper respiratory tract inflammation in 2008 (referred to herein as the UR strain) [21] . The clinical samples were obtained by the local health authorities of Kochi Prefecture (AM strain) or Gunma Prefecture (UR strain) in 2008 for the surveillance of viral diseases in Japan, but we used clinical isolates that were supplied from these local health authorities. Thus, this study did not involve research on human subjects and thus does not need the approval of the Ethical Review Board. After isolation in non-human primate LLC-MK 2 cells (AM strain) or in human HEp-2 cells (UR strain), the stock viruses were passaged (three times for the AM strain and once for the UR strain) in LLC-MK 2 cells cultured in minimal essential medium (MEM) supplemented with 2% fetal bovine serum, 100 U/ml penicillin, 100 μg/ml of streptomycin, and 1.5 μg/ml of Fungizone Antimycotic (Gibco, Life Technologies Corporation, Carlsbad, CA) (referred to herein as 2MEM). The virus-infected LLC-MK 2 cells were disrupted using three freeze-thaw cycles and centrifuged at 2,000 × g for 15 min. The supernatant was stored at 80°C until required. Titers of the stock viruses were expressed as 50% of the cell culture infectious dose (CCID 50 )/ml in LLC-MK 2 cells, which was calculated using the Behrens-Kärber method. All work with infectious SAFV-3 was performed under biosafety level two conditions. SAFV-3 UR strain genome sequencing SAFV-3 UR strain RNA was extracted from virus-infected cell cultures using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). Reverse transcriptase (RT)-PCR was performed with the OneStep RT-PCR Kit (Qiagen) using specific primers [27, 44] . The amplified DNA PCR products were purified using MonoFas DNA Purification Kit I (GL Sciences Inc., Tokyo, Japan) and then sequenced using an ABI 3130 Genetic Analyzer (Applied Biosystems, Life Technologies Corporation). The nucleotide sequences of the SAFV-3 UR strain were analyzed using Sequencher software (ver. 4.10.1, Gene Codes Corporation, Ann Arbor, MI). All nucleotide sequences analyzed in this study were submitted to the DNA Data Bank of Japan.

Pregnant and 5-week-old female ddY mice, an outbred strain, and 5-week-old female BALB/c mice, an inbread strain, were purchased from Japan SLC (Shizuoka, Japan). The ddY strain was maintained as a closed colony and shows good reproductive performance and growth [45] .

Within 24 h of birth, neonatal ddY mice were inoculated intracerebrally or intraperitoneally with the SAFV-3 AM or UR strains (10 4 CCID 50 in 10 μl per mouse). 2MEM was used as a negative control and as the diluent whenever needed. The mice were observed for clinical manifestations, and their body weight was measured daily for 21 days. Additional inoculated animals were sacrificed at various time points to examine virus replication and pathology (n = 3, 4, or 7 mice per group).

Six-week-old ddY and BALB/c mice (referred to hereafter as young mice) were anesthetized with isoflurane and inoculated intracerebrally with the AM or UR strains of SAFV-3 (10 4 CCID 50 in 50 μl). The mice were monitored for clinical signs of infection, and body weight changes were measured for 8 (ddY) or 60 (BALB/c) days. The mice were sacrificed under excess isoflurane anesthesia on 3, 8, 21, or 60 days post-inoculation (p.i.) and subjected to pathological analysis (n = 3-6 per group). The young ddY and BALB/c mice used as negative controls were inoculated intracerebrally with 2MEM.

Young BALB/c mice (n = 10 or 13 mice per group) were inoculated intracerebrally (10 4 CCID 50 in 50 μl per mouse), intraperitoneally (10 4 CCID 50 in 100 μl), intravenously (10 4 CCID 50 in 100 μl), intranasally (10 4 CCID 50 in 20 μl), or orally (10 4 CCID 50 in 100 μl containing 5% sucrose) with the AM or UR strains. 2MEM was used as a negative control and as the diluent whenever needed. Intracerebral inoculation was conducted under isoflurane anesthesia, and intranasal inoculation was performed under a mixture of ketamine and xylazine anesthesia [46] . Before oral inoculation, animals were deprived of water for 6 or more hours. Feces were obtained from orally-inoculated animals on Days 3 and 8 p.i. and used for viral isolation. All inoculated mice were observed for clinical signs of infection and body weight was measured daily for 21 days (n = 3, 4, or 5 mice per group). The mice were sacrificed under excess isoflurane anesthesia on Days 3, 8, and 21 p.i. and examined using virological and pathological methods.

Animal studies were carried out in strict accordance with the Guidelines for Proper Conduct of Animal Experiments of the Science Council of Japan. The animal experiments were conducted in strict compliance with animal husbandry and welfare regulations. All animal experiments were approved by the Committee on Experimental Animals at the National Institute of Infectious Diseases in Japan (approval No. 211028, 212031, 112075, 113090, and 114102) , and all experimental animals were handled in biosafety level two animal facilities according to the guidelines of this committee. If necessary, viral inoculations were performed under anesthesia, and all efforts were made to minimize potential pain and distress. After inoculation, animals were monitored once a day during the study. The humane endpoint was used for all mice displaying the clinical diagnostic criteria of severe central nervous system (CNS) stress, such as circling, blindness, and convulsion, and more than 20% weight loss. Animals were euthanized under anesthesia if severe disease symptoms or weight loss was observed, while no animals became severely ill or moribund at any point prior to the experimental endpoint.

Neonatal mice were sacrificed by exposure to excess isoflurane and perfused with 10% phosphate buffered formalin (injected directly into the heart). Harvested tissues were immersed in a formalin solution overnight. The whole head, including the brain, was cut into sagittal sections, which were then immersed in a formalin solution for 2 or 3 days. Young mice were also sacrificed by exposure to excess isoflurane and exsanguinated (via the heart). The head, including the brain, the spine including the spinal cord, muscle, heart, lungs, liver, spleen, kidneys, pancreas, stomach, and intestine were harvested and immersed in a formalin solution for 2 or 3 days. After fixation, the tissues were dehydrated in ethanol (30% to 50% solutions) and decalcified in a buffered EDTA 2Na solution (Dojindo, Kumamoto, Japan) as required. The tissue samples were then embedded in paraffin and stained with hematoxylin and eosin (H&E). The brain and spinal cord tissues were also stained with Klüver-Barrera Luxol fast blue staining (KB-LFB).

A polymer-based detection system was used for immunohistochemical analysis. For antigen retrieval, deparaffinized sections were placed in a retrieval solution (pH 6) (Nichirei Biosciences, Inc., Tokyo, Japan) and heated to 121°C for 10 min in an autoclave. After washing in phosphate buffered saline (PBS), the sections were treated with 0.3% hydrogen peroxide in methanol for 30 min to quench endogenous peroxidase activity. After further washing, the sections were incubated at room temperature in 10% normal goat serum (Dako, Glostrup, Denmark) for 5 min, followed by an overnight incubation at 4°C with the primary anti-SAFV-3 polyclonal antibody or anti Iba1 polyclonal antibody (Wako Pure Chemical Industries, Ltd, Osaka, Japan). The SAFV-3 polyclonal antibody was used alongside serum from a rabbit hyper-immunized with the SAFV-3 AM strain [36] . After further washing in PBS, the sections were incubated with Nichirei-Histofine Simple Stain Mouse MAX PO (R) (Nichirei Biosciences) according to the manufacturer's instructions. Peroxidase activity was detected with 3, 3'-diaminobenzidine (Sigma-Aldrich, St. Louis, MO), and the sections were counterstained with hematoxylin.

To characterize the virus-infected cells, paraffin-embedded tissues were subjected to a double immunofluorescence staining procedure using a rabbit antiserum against SAFV-3, and neuronal markers such as mouse or rat monoclonal antibodies against Musashi-1 (clone D270-3; Medical & Biological Laboratories, Nagoya, Japan), anti-glial fibrillary acidic protein (GFAP) (clone GA5; Merck Millipore, Billerica, MA), anti-glutamate-aspartate transporter (GLAST) (clone ACSA-1; Miltenyi Biotec, Auburn, CA), anti-brain lipid binding protein (BLBP) (clone AT1D1; Abcam, Cambridge, UK), microtubule associated protein-2 (MAP-2) (clone A60; Millipore), anti-myelin 2,3-cyclic nucleotide 3-phosphodiesterase (CNPase) (SMI91; BioLegend, San Diego, CA), myelin-associated glycoprotein (MAG) (ab89780; Abcam), and anticytokeratin (clone AE3; Chemicon, Merck Millipore), and lectin biotinylated GRIFFONIA SIMPLICIFOLIA (GS-lectin) (Vector, Southfield MI). The antigens were retrieved by autoclaving the sections in retrieval solution (pH 6.0) (Nichirei Biosciences) at 121°C for 10 min. The sections were incubated with the primary monoclonal antibody (against Musashi-1, GFAP, GLAST, BLBP, MAP-2, CNPase, MAG, or cytokeratin) or GS-lectin, followed by incubation with antiserum against the SAFV-3 antigen. Goat anti-rabbit Alexa Fluor 568 (to detect viral antigen-binding sites), goat anti-rat Alexa Fluor 488 (to detect antibodies bound to Musashi-1), goat anti-mouse Alexa Fluor 488 (to detect antibodies bound to GFAP, GLAST, BLBP, MAP-2, CNPase, MAG, or cytokeratin) and streptavidin, Alexa Fluor 488 conjugate (to detect GS-lectin) were used as secondary antibodies (all secondary antibodies were obtained from Molecular Probes, Life Technologies Corporation). The sections were incubated with the antibodies for 30 min at room temperature before being mounted in SlowFade Gold antifade reagent containing 4', 6-diamidino-2-phenylindole (DAPI; Molecular Probes, Life Technologies Corporation). Fluorescence images were captured under a laser scanning confocal microscope (FV1000-D, Olympus, Tokyo, Japan).

Viral RNA was detected in sections of paraffin-embedded mouse brain by RNA in situ hybridization using the QuantiGene ViewRNA ISH Tissue Assay (Affymetrix, Santa Clara, CA) according to the manufacturer's instructions, with some modifications [47] . The QuantiGene ViewRNA Probes Sets were designed using the whole gene for the SAFV-3 AM strain (accession no. HQ902242). Prior to RNA in situ hybridization, total RNA was extracted using a Pure-Link FFPE RNA Isolation Kit (Invitrogen, Life Technologies Corporation) to determine whether the viral RNA was preserved in the paraffin-embedded specimens. The RNA was then treated with the reagents provided in the TURBO DNA-free Kit (Ambion, Life Technologies Corporation) to remove contaminating DNA. RNA-extracted samples were subjected to realtime PCR to detect SAFV-3, and appropriate samples were selected for RNA in situ hybridization. After deparaffinization, the sections were boiled with pretreatment solution for 10 min and treated with protease QF at 40°C for 10 min. The sections were incubated with the QuantiGene ViewRNA Probe Set at 40°C for 2 h and then hybridized with PreAmplifier Mix QT at 40°C for 25 min. After treatment with Amplifier Mix QT at 40°C for 15 min, the sections were reacted with the labeled probe conjugated to alkaline phosphatase. After receiving alkaline phosphatase enhancer treatment, the sections were stained using a Warp Red Chromogen Kit (Biocare Medical, Concord, CA).

Reverse transcriptase PCR (RT-PCR) and real-time RT-PCR of the SAFV-3 genome Neonatal mice were sacrificed by exposure to excess isoflurane, and blood was obtained by cardiac puncture. Total RNA was extracted using the TRIzol Plus RNA Purification Kit (Ambion, Life Technologies Corporation). The brains from the ddY neonatal mice were removed from the skull, washed three times in PBS, and then separated into two parts (the cerebellum and the remaining brain, including the cerebrum and brain stem). The brains from young BALB/c mice were divided into three parts (the cerebrum, brain stem, and cerebellum) to quantify the viral RNA genome. Tissue samples (except samples of neonatal mice cerebellum) were homogenized using Lysing Matrix A (MP Biomedicals, Santa Ana, CA) and diluted in 2MEM to yield 10% homogenates. Samples of neonatal cerebellum were also homogenized using Lysing Matrix A (MP Biomedicals) in 1 ml of 2MEM. After centrifugation at 8,000 × g for 5 min, the supernatants were subjected to PCR to detect the viral genome. Total RNA was extracted from the supernatants of tissue homogenates generated from the organs of young mice and the brains of the neonatal mice using an RNeasy Plus Mini Kit (Qiagen). Contaminating DNA was removed from the extracted RNA using a TURBO DNA-free Kit (Ambion, Life Technologies Corporation). Nested RT-PCR was performed to detect the SAFV viral genome (the conserved 5' non-coding region; accession no. EF165067), and the product was amplified using OneStep RT-PCR (Qiagen) and TaKaRa Ex Taq (Takara Bio, Shiga, Japan) kits [27] . The first and second primer sets are listed in Table 1 . The nested RT-PCR protocol was as follows: the firstround of the RT-PCR was conducted for 30 min at 50°C (for reverse transcription) and 15 min at 95°C (for Taq polymerase activation), followed by 35 cycles at 94°C for 30 sec, 60°C for 30 sec, and 72°C for 1 min. The second-round of PCR was conducted for 3 min at 95°C, followed by 35 cycles at 94°C for 30 sec, 60°C for 30 sec, and 72°C for 1 min.

Primer Express software (Applied Biosystems) was used to design the primers and probes for real-time RT-PCR, all of which recognized the SAFV-3 viral protein 1 (VP1) region (accession no. HQ902242). The expression of type 1 interferon (IFN) mRNA was examined using real-time RT-PCR methods designed to detect mouse IFN-α4 and IFN-β mRNA [46, 48] . Mouse beta-actin mRNA (a housekeeping gene) was also quantified. The Taqman probe and primer sets are listed in Table 1 . The real-time RT-PCR conditions used were previously published by Katano et al. [49] .

Sera were collected by centrifugation and inactivated by heating at 56°C for 30 min. Sera were titrated (in duplicate) from 1:16 to 1:256 in 96-well plates and reacted with inoculated 100 CCID 50 of SAFV-3 at 37°C for 2 h before LLC-MK 2 cells were added to each well. Titers of neutralization antibodies were assessed against each virus, which was identical to the virus used in the experimental infection of mice. Cells were incubated at 37°C for 2 weeks, during which time they were examined twice for the presence of viral cytopathic effects. The neutralizing antibody titer was determined as the reciprocal of the highest dilution at which no cytopathic effect was observed.

Data are expressed as the mean and standard error of the mean. Statistical analysis was performed using Graph Pad Prism 5 software (GraphPad Software Inc., La Jolla, CA). Intergroup comparisons were performed using unpaired t-tests or one-way analysis of variance (ANOVA), followed by Tukey's post test. A P-value <0.05 was considered statistically significant.

The complete genomes for the AM and UR strains of SAFV-3 were compared. The nucleotide sequence for the complete genome of the AM strain (accession no. HQ902242) was 98.6% identical to that of the UR strain (accession no. AB983594). The AM strain genome differenced from the UR strain with respect to 114 nucleotides, eight of which were non-synonymous. Regarding the structural proteins, the VP2 protein of the AM and UR strains differed by only a single amino acid (at position 269: Thr in AM and Asn in UR). By contrast, the non-structural proteins showed differences at the following seven amino Nested RT-PCR for Saffold virus

To investigate the neurovirulence of SAFV-3, neonatal ddY mice were inoculated intracerebrally with the AM or UR strain of SAFV-3. We found that 50% of the AM-inoculated neonatal mice developed mild neurological signs, such as elevated tails, ataxia, and rolling, on Days 7 and 8 p.i.; however, all mice recovered rapidly. The rate of weight gained in the UR-inoculated neonatal mice was significantly lower than that in the 2MEM-inoculated control mice during the observation period, whereas that in AM-inoculated neonatal mice was equal to that in the 2MEM-inoculated control mice (Fig 1) . The observed neuronal deficits suggested that viral infection might affect the cerebellum in neonatal mice, especially in those inoculated with the AM strain. This result suggested that both SAFV-3 strains were mildly virulent in neonatal mice. Additionally, the virulence of the two SAFV-3 strains differed in neonatal mice after intracerebral inoculation.

We also used histopathological and immunohistochemical approaches to examine the ability of the SAFV-3 virus to infect neonatal mice after intracerebral inoculation (Table 2 and Fig 2) . On Day 3 p.i., the AM-inoculated mice showed mild or no histopathological changes in the brain or other major organs, although viral antigens were detected in the brain, spinal cord, and skeletal muscle ( Table 2 and Fig 2A) . UR-inoculated mice showed significant histopathological changes in the brain and tooth germ, including cellular degeneration with condensed nuclei and necrosis with slight cellular infiltration ( Table 2 and Fig 2A) . SAFV antigens were detected in degenerated neural cells (which showed eccentric nuclei) around the lateral ventricle, which was the virus inoculation site, and in the undeveloped medulla of the cerebellum (Fig 2A, insets) . In addition, a few neural cells within the spinal cord, and skeletal muscle cells throughout the body, were positive for viral antigens (Table 2 ). However, none of the large pyramidal neurons in the brain and spinal cord were positive for viral antigens. Moreover, viral antigens were detected in the oral cavity of UR-inoculated mice, but the histopathological changes were very weak. The number of viral antigen-positive cells in the cerebellum lobule of AM-inoculated mice was approximately five times greater than that in UR-inoculated mice on Day 3 p.i., and 16 times greater on Day 7 p.i. (Fig 2B) . RNA in situ hybridization using paraffin-embedded brain tissues harvested from neonatal mice at 3 days p.i. revealed that viral RNA was present in the ventricle, cerebrum, and brain stem of mice inoculated with either virus strain ( Fig 2C) . Notably, the cerebellum in AM-inoculated mice, but not in UR-inoculated mice, was positive for viral RNA. The distribution of viral antigens correlated with that of viral RNA in the brain. On Day 3 p.i., mild perivascular cuffing was more common in the cerebrum/brain stem of UR-inoculated mice than in that of AM-inoculated mice; however, no perivascular cuffing was observed in the cerebellum of either group (S1 Fig, upper panels) . Appreciable perivascular cuffing and vacuolation in the neuropil were detected in the brains stem of UR-inoculated mice on Day 21 p.i. (S1 Fig, lower panels; Table 2 ), with two of the four mice showing severe focal necrosis in the thalamus. In addition, mild meningitis and perivascular cuffing was observed in the spinal cords of UR-inoculated mice but not in AM-inoculated mice (Fig 3A) . Foci of demyelination with Iba1 + microglia infiltrations were observed in the white matter of the spinal cords of UR-inoculated mice but not of AM-inoculated mice (Fig 3B) . Histopathologically, a few microglia phagocytized viral antigens in the lesion of the brainstem and spinal cord.

Double immunofluorescence staining indicated that GLAST + or GFAP + glial cells and Musashi-1 + neural progenitor cells in the cerebellum of AM-inoculated animals were positive for viral antigens (Fig 4A) . The Musashi-1 + neural progenitor cells, as well as the neuroepithelial cells and GLAST + or GFAP + glial cells around the ventricle, were also positive in UR-inoculated mice (Fig 4B) . BLBP + radial astroglia cells both in the AM-and in the UR-inoculated mice were positive for viral antigens, but MAP-2 + neuronal cells, CNPase + or MAG + oligodendroglia cells, and GS-lectin + microglia cells were negative for viral antigens. In addition, the cytokeratin-positive epithelial cells in the oral cavity of UR-inoculated mice were positive for viral antigens (Fig 4C) , as were the calbindin-positive cells in the tooth germ [50] . The skeletal infected both glial and epithelial cells. However, histopathological examination suggested that the AM strain replicated in the cerebellum (Fig 2B and 2C) to a greater extent than the UR strain after intracerebral inoculation.

Histopathological examination and the observed neuronal deficits suggested that viremia might occur after virus inoculation, particularly in mice inoculated with the UR strain, and that viral infection might affect the cerebellum in neonatal mice, particularly those inoculated with the AM strain. Thus, viral replication was measured separately in the blood, cerebellum, and other brain regions (cerebrum and brain stem, referred to hereafter as the cerebrum/brain stem). Real-time PCR revealed that viral RNA levels in the blood of SAFV-3-inoculated mice peaked at 3-5 days p.i., although the levels were higher in UR-inoculated mice (Fig 5A) . Thus, viremia was detected in both groups post-inoculation, although the levels were different. The levels of viral RNA in the cerebrum/brain stem and cerebellum of both groups increased after Day 3 p.i. Notably, on Days 3 and 5 p.i., the levels of viral RNA in the cerebrum/brain stem of mice infected with the UR strain were significantly higher than those in mice infected with the AM strain. By contrast, on Day 5 p.i., the levels of viral RNA in the cerebellum of mice infected with the AM strain were significantly higher than those in the cerebellum of mice infected with the UR strain ( Fig 5B) .

We next examined the anti-viral responses to SAFV infection in the brains of neonatal mice. IFN-β expression in the brains of both groups increased at 3 days p.i.; however, levels in the brains of UR-inoculated mice were significantly higher than those in the brains of AMinoculated mice (Fig 5C) . Thus, intracerebral inoculation of SAFV-3 induced an anti-viral immune response (type 1 IFN expression) in the neonatal mouse brain. In addition, the UR strain induced a stronger type 1 IFN response and longer-term inflammatory infiltration than the AM strain.

Taken together, these results suggest that both SAFV-3 strains infect the neonatal mouse brain. Additionally, the two SAFV-3 strains showed differing infectivity and virulence after intracerebral inoculation into neonatal mice.

We also assessed the neurotropism of SAFV-3 after intraperitoneal inoculation into neonatal ddY mice. The mice showed no obvious clinical signs after intraperitoneal inoculation with either the AM or UR strain of SAFV-3. However, histopathological and immunohistochemical examination revealed that these strains invaded and infected the CNS on Days 3 and 7 p.i. (S1 Table and The number of viral RNA copies was measured in the blood (A) and cerebrum/brain stem and cerebellum (B) of each mouse on Days 1, 3, 5, 7, and 10 postinoculation (p.i.) (n = 4 mice per group for each day). The number of viral RNA copies is expressed relative to the number of mouse beta-actin RNA copies. Viral RNA levels in the blood and brain peaked at 3 to 5 Days p.i. in mice inoculated with AM and UR strains (*P < 0.05, **P < 0.01, and ***P < 0.001; unpaired t-test). (C) Real-time reverse transcriptase (RT)-PCR quantification of interferon beta (IFN-β) mRNA expression in the brains of SAFV-inoculated neonatal mice (n = 3-4 mice per group). The number of IFN-β RNA copies is expressed relative to the number of mouse beta-actin RNA copies. On Day 3 p. i., UR-inoculated mice showed greater expression of IFN-β than AM-inoculated mice (*P < 0.05 and ***P < 0.001; one-way ANOVA). the UR strain showed a greater capacity to infect the CNS than the AM strain after intraperitoneal inoculation.

Young ddY mice were inoculated intracerebrally with the AM or UR strains to examine the neurovirulence of SAFV-3. At Day 6 p.i., the young UR-inoculated ddY mice gained significantly less body weight than the 2MEM-inoculated controls. There was no difference in body weight between AM-inoculated and 2MEM-inoculated ddY mice. None of the mice showed obvious clinical manifestations. Both AM-and UR-inoculated young ddY mice showed histopathological evidence of perivascular inflammation and neural cell degeneration with eccentric nuclei (S5 Fig) . Viral antigen-positive cells were observed on Day 3 p.i.; double immunofluorescence staining revealed that the these cells were GLAST + or GFAP + glial cells and Musashi-1 + progenitor neural cells from within the cerebral medulla and GFAP + glial cells from the cerebellum cortex (S6 Fig). In addition, viral antigens were detected in the cerebellum of two of three mice in each virus-inoculated group on Day 3 p.i. However, none of the large pyramidal neurons in the CNS were positive for viral antigens. On Day 8 p.i., all mice in both virus-inoculated groups showed meningitis and perivascular cuffing in the brain and brain stem, but not in the cerebellum. No histopathological changes were noted in the oral mucosa or tooth germ in AM-and UR-inoculated mice. These results suggested that SAFV-3 was mildly neurovirulent in young ddY mice.

We next examined the neurovirulence and neurotropism of SAFV-3 in young BALB/c mice, which are Th-2-prone inbred mice, following intracerebral inoculation. AM-and UR-inoculated mice lost significantly more body weight than 2MEM-inoculated control mice between 1 and 3 days p.i. (Fig 6) . However, mice in both groups regained the lost weight during the observation period and exhibited no obvious clinical signs. Histopathological and immunohistochemical analyses on Day 3 p.i. revealed that the degenerated and necrotic nerve cells in the brains were positive for viral antigens (Fig 7 and Table 3 ). All of the AM-and UR-inoculated mice showed signs of meningitis. Viral antigen-positive cells were detected in the cerebral medulla, but not in the cortex, in both groups. A greater number of viral antigen-positive cells were observed in the brains of UR-inoculated mice than in the brains of AM-inoculated mice (Fig 7) . However, whereas viral antigens were detected in all of the AM-inoculated mice along with mild inflammation in the molecular layer of the cerebellar cortex on Day 3 p.i., viral antigens were detected in only two of the five UR-inoculated mice (Fig 7 and Table 3 ). Virus antigen-positive cells were detected in the spinal cord of a few mice on Day 3 p.i. (Table 3) . The viral antigen-positive cells in the cerebral medulla of both groups were identified as GLAST + or GFAP + glial cells and Musashi-1 + progenitor neural cells by double immunofluorescence staining (S7 Fig). Viral RNA was detectable in the brains of mice in both groups on Days 3, 8, and 21 p.i., although at very low levels (Fig 8A) . The expression of IFN α4 and β mRNA in the cerebrum of UR-inoculated mice was higher than that in AM-inoculated mice on Day 3 p.i. (Fig 8B) ; however, the number of IFN α4 and β mRNA copies in the cerebellum of AM-inoculated mice was significantly higher than that in the cerebellum of UR-inoculated mice (Fig 8B) .

Histopathologically, inflammatory infiltrations were detected in the cerebrum of AM-and UR-inoculated mice on Day 3 p.i. Although no type 1 IFN was expressed on Day 8 p.i., inflammatory infiltration was observed in the cerebellum of all AM-inoculated mice, whereas it was observed only in the cerebellum of one UR-inoculated mice (Fig 9) . On Day 21 p.i., mild perivascular cuffing was observed in the spinal cord of two of the four UR-inoculated mice. None of the mice displayed histopathological changes in muscle, oral mucosa, or tooth germ (Table 3) . On Day 60 p.i., neither AM-nor UR-inoculated mice had viral antigen-positive cells in the brain. However, they were positive for viral RNA; one of the six brains from AM-inoculated mice was positive and three of six brains from UR-inoculated mice were positive in the second round PCR of nested RT-PCR for SAFV (S2 Table) . However, viral RNA was not detected in the paraffin embedded brain tissues of either AM-or UR-inoculated mice on Day 60 p.i. These results suggest that the viral RNA level was too low to be detectable by nested RT-PCR in the CNS on 60 day p.i. Furthermore, only two of the six UR-inoculated mice showed mild inflammatory infiltration, without a demyelinating lesion, in the white matter of the spinal cord and the lateral ventricle.

These results indicated that SAFV-3 was only mildly neurovirulent in young ddY and BALB/c mice when inoculated intracerebrally. Both strains showed low infectivity and neurotropism in the brains of these young mice; however, the immune response of mice infected with the two SAFV-3 strains differed, particularly that in the cerebellum of young BALB/c mice. The observed body weight changes, coupled with the inflammatory infiltrates in the cerebellum, suggest that young ddY and BALB/c mice have different susceptibilities or immune responses to infection by SAFV-3 isolates. Young BALB/c mice (Th-2 prone inbred mice) showed a stronger immune response to SAFV-3 infection than ddY mice.

Finally, we examined the neuroinvasiveness and infectivity of SAFV-3 by inoculating BALB/c mice via different routes. Because the oral cavity of neonatal mice was positive for viral antigens after intracerebral inoculation with the UR strain (Fig 2A) , we speculated that SAFV-3 might infect young mice through the mucosa. To explore this hypothesis, we infected young BALB/c mice with the AM and UR strains of SAFV-3 (10 4 CCID 50 per mouse) via various routes (intraperitoneal, intravenous, oral, and intranasal). None of the animals showed clinical neurological signs. In addition, no histopathological changes were observed in the CNS (including the olfactory bulb) or other organs on Days 3, 8, and 21 p.i. However, on Day 3 post-intraperitoneal inoculation, immunohistochemical analysis revealed viral antigen-positive cells in the pancreas of all three AM-inoculated mice and one of the three UR-inoculated mice (S8 Fig). Similarly, on Day 3 post-intravenous inoculation of UR, viral antigens were detected in the pancreas in one of three mice. However, no histopathological changes or viral antigens were observed in the major organs after oral or intranasal inoculation with either virus strain. Interestingly, very mild and focal inflammatory infiltrations (in the absence of viral antigen) were seen in the nasal mucosa of mice inoculated with UR via the oral and intranasal routes. No virus was detected in the feces on Days 3 and 8 after oral infection. We then measured the levels of anti-SAFV-neutralizing antibodies in the sera of mice on Day 21 p.i., to examine seroconversion against each inoculated strain. All of the young BALB/c mice seroconverted after intracerebral, intraperitoneal, or intravenous inoculation with SAFV-3 (Fig 10) . Notably, oral and intranasal inoculation with the UR strain induced seroconversion in young BALB/c mice. By contrast, mice orally administered the AM strain did not seroconvert. After intranasal administration, two out of four animals showed low levels of neutralizing antibodies against the inoculated virus. These results suggested that the UR strain, but not the AM strain, infected young BALB/ c mice through "natural" routes, including the oral and nasal mucosa.

Here, we showed that two clinical SAFV-3 isolates, an AM strain isolated from a patient with aseptic meningitis and a UR strain isolated from a patient with an upper respiratory infection, induced mild neuropathogenesis in neonatal and young mice. Neither strain caused severe neurological manifestations, such as paralysis or fatal encephalitis, in neonatal or young mice. Neonatal ddY mice intracerebrally inoculated with the UR strain showed poor (but not fatal) weight gain, and neonatal ddY mice intracerebrally inoculated with the AM strain showed mild clinical signs, such as tremors; however, these signs were minor and transient. Immunohistochemical analysis revealed that both SAFV-3 isolates infected glial cells, but not large pyramidal neurons, in neonatal mice. However, histopathological investigation revealed that both strains induced an aseptic meningitis (lasting at least 3 weeks) in the brains of neonatal Number of viral IFN-α4 and IFN-β RNA copies relative to that of mouse beta-actin copies (***P < 0.001; one-way ANOVA). On Day 3, the expression of IFN-α4 and IFN-β in the cerebellum of AM-inoculated mice was higher than that in UR-inoculated mice.

doi:10.1371/journal.pone.0148184.g008 mice after intracerebral or intraperitoneal inoculation; thus both SAFV-3 strains were mildly neuropathogenic, neurotropic, and neurovirulent in neonatal ddY mice. There were some differences between the two SAFV-3 strains in terms of infectivity and cell tropism in the murine neonatal model. For instance, the ependymal cells and mucosal epithelia of the oral cavity and the tooth germ in neonatal mice were infected following intracerebral infection with the UR, but not the AM, strain. In addition, the UR strain infected the mucosa in young mice following intranasal or oral inoculation; thus this animal model recapitulates the potential infectivity of the UR strain observed in human upper respiratory tract infections. Therefore, both the neonatal and young mouse models will be useful for future studies aimed at elucidating the pathogenesis of different SAFV-3 strains. The present study highlights the similarities and differences between SAFV-3 and other cardioviruses. Theiler's murine encephalomyelitis virus (TMEV) is a common enteric pathogen in mice and causes poliomyelitis-like flaccid paralysis and a chronic progressive white matterdemyelinating disease, which is considered a relevant viral infection model of human multiple sclerosis [51] [52] [53] . Neurovirulent strains of TMEV mainly infect the cerebral cortex, hippocampus, and spinal cord after experimental inoculation [54] [55] [56] . Virus-mediated neurological disorders are caused by direct lytic infection of neurons and oligodendroglia and by secondary immune-mediated destruction of infected cells [57] . We found that the UR-inoculated neonatal mice intracerebrally showed foci of demyelination with microglia infiltrations in the spinal cord on day 21 p.i. This seems to be an early phase of demyelination after viral infection, which is likely to be due to TMEV infection. However, SAFV-3 infected adult mice did not show any lesion associated with demyelinating disease. Interestingly, the SAFV-3 strains examined herein were neurotropic for glial cells in the cerebellum but not for oligodendroglia; however, no study has reported a similar tropism for TMEV [54] . Additionally, there is no evidence for an association between SAFV and human multiple sclerosis [13, 58] . The pathological process of demyelination may be different between SAFV and TMEV. In the present study, SAFV-3 infected the pancreas of young mice after intraperitoneal inoculation. Another cardiovirus, Encephalomyocarditis virus, also infects the pancreas of mice and causes type 1 diabetes [3, 5] ; thus, SAFV-3 may have the potential to induce diabetes in mice. Similar to other murine enteric cardioviruses, the UR strain infected young mice via the mucosal route. Therefore, this mucosal infection model may mimic SAFV infection in humans and prove useful for furthering our understanding of the pathogenesis of SAFV. However, the routes of viral infection following oral and intranasal inoculation remain unclear.

The difference between the two SAFV-3 strains in terms of their ability to infect epithelial cells and neurons in the cerebellum may be related to the specific receptors used, or to factors that affect viral replication. Although the AM and UR strains differed by only a single amino acid in the VP2 protein; there were seven amino acid differences in the non-structural proteins (L, 2A, 2C, 3B, 3C, and 3D). The capsid protein of cardioviruses contains four loop structures, loops I and II in VP1, and puffs A and B in VP2, all of which are involved in receptor binding [13, 59, 60] . The leader protein of TMEV plays an important role in viral replication by inhibiting alpha/beta interferon production [61, 62] and inducing apoptosis [63, 64] . It also plays a role in neurovirulence [65] . In the present study, the AM and UR strains differed in terms of virus replication and induction of type 1 IFN expression in infected mice. The dissimilarity in the amino acid sequences of the two SAFV-3 isolates may have contributed to the observed differences in tissue tropism, viral replication, and type 1 IFN expression in the two mouse models. Notably, the expression of type 1 IFN in the cerebellum of AM-inoculated young mice was significantly greater than that in UR-inoculated mice. This discrepancy could be related to differences in cell tropism in these mice.

The AM strain showed greater tropism for the cerebellum than the UR strain. Nielsen et al. detected SAFV in the CSF and fecal samples from a 16-month-old child suffering from monosymptomatic ataxia caused by cerebellitis [35] . The neural signs, fluctuating from an insecure gait to walking into things and falling, remitted completely without sequelae during the next 2 months. In the present study, AM-inoculated neonatal mice showed neurological signs such as ataxia, rolling, and elevated tails on Days 7 and 8 p.i.; these mice also recovered rapidly. Interestingly, viral antigens were detected in glial cells in the cerebellum (aka Bergmann glia) but not in neural (Purkinje) cells. Bergmann glia and Purkinje cells, which construct glial microdomains, interact with growing neurites [66] . Cerebellar ataxia is generally thought to be caused by the loss of Purkinje cells. Therefore, we speculate that the SAFV-infected Bergmann glia in the present study affected the function of the Purkinje cells, leading to the cerebellar ataxia observed in neonatal mice.

Highly neurotropic viruses such as poliovirus, enterovirus 71, and neurovirulent flaviviruses, infect the CNS and cause viral meningitis or encephalomyelitis, which is characterized by neuronal damage and perivascular inflammation [67] [68] [69] [70] . The preferential target of these neurotropic viruses is the large pyramidal neurons in the human CNS (and in that of animal models) [38, 71, 72] . In this study, the two SAFV-3 strains only infected neural progenitor cells and glial cells around the lateral ventricles and cerebellum in both neonatal and young mice. Neither infected the large pyramidal neurons in the brain cortex, brain stem, spinal cord, and cerebellum. This tropism of SAFV-3 could reflect the neuropathogenesis, such as meningitis and cuffing, observed in the brains of these mice (and possibly that observed in the human brain).

In summary, we examined the neuropathogenesis of SAFV-3 in two mouse models and found that although SAFV-3 is a candidate neuropathogenic agent, it is different from the highly neurotropic enteroviruses, such as poliovirus, enterovirus 71, and coxsackieviruses A and B. The present in vivo comparative study using virological, pathological, and immunological methods to examine the effects of clinical viral isolates has further elucidated the pathogenesis of SAFV. However, future studies on the neuropathogenesis of SAFV will be required for complete understanding of this virus.

Supporting Information S1 Fig. Inflammatory infiltrate in the brains of neonatal ddY mice after intracerebral inoculation with SAFV-3. Within 24 h of birth, neonatal ddY mice were inoculated intracerebrally with 10 4 CCID 50 (cell culture infectious dose) of the aseptic meningitis (AM) or upper respiratory (UR) strains of SAFV-3. Representative histopathological images of inflammatory infiltration in the brains of neonatal mice on Days 3 and 21 post-inoculation (p.i.) are shown (n = 3, 4, or 7 mice per group). Hematoxylin and eosin (H&E) staining. Bars, 50 μm. On Day 3 p.i., swollen vascular endothelial cells (red arrow, inset) and a mild inflammatory infiltrate with microglia (blue arrow, inset) were seen in the cerebrum/brain stem of UR-inoculated mice (black arrows, panels in second row), but not in that of AM-inoculated mice (panels in first row). On Day 21 p.i., perivascular cuffing with mononuclear cell infiltration was observed in the brain stem/cerebellum of UR-inoculated mice (arrows and insets of fourth row) but not in those of AM-inoculated mice (panels in third row). Original magnification, 400×; insets, 1,000×. 

Discovery of a novel human picornavirus in a stool sample from a pediatric patient presenting with fever of unknown origin

The pathogenesis of experimental infections with encephalomyocarditis (EMC) virus

Diabetes mellitus: induction in mice by encephalomyocarditis virus. Science

Animal model:Theiler's virus infection in mice

Virus-induced diabetes mellitus. XVIII. Inhibition by a nondiabetogenic variant of encephalomyocarditis virus

Theiler's murine encephalomyelitis: a model of demyelination and persistence of virus

An encephalomyocarditis virus epizootic in a baboon colony

Encephalomyocarditis virus infections in an Australian zoo

Human febrile illness caused by encephalomyocarditis virus infection

Encephalomyocarditis virus infection in an Italian zoo

Prevalence and risk factors for encephalomyocarditis virus infection in Peru. Vector Borne Zoonotic Dis

Epizootic myocarditis associated with encephalomyocarditis virus in a group of rhesus macaques (Macaca mulatta)

Identification of cardioviruses related to Theiler's murine encephalomyelitis virus in human infections

Circulation of 3 lineages of a novel Saffold cardiovirus in humans

Cardioviruses are genetically diverse and cause common enteric infections in South Asian children

Saffold cardiovirus in children with acute gastroenteritis

New Saffold cardiovirus in children

Saffold virus, a human Theiler's-like cardiovirus, is ubiquitous and causes infection early in life

Cultivation and serological characterization of a human Theiler's-like cardiovirus associated with diarrheal disease

Saffold cardioviruses of 3 lineages in children with respiratory tract infections

Sequencing and phylogenetic analyses of Saffold cardiovirus (SAFV) genotype 3 isolates from children with upper respiratory infection in Gunma

Saffold virus infection in children

Molecular detection of Saffold Virus in children in Shanghai

Seroepidemiology of Saffold cardiovirus type 2

Saffold cardiovirus infection in children associated with respiratory disease and its similarity to coxsackievirus infection

Seroepidemiology of Saffold cardiovirus (SAFV) genotype 3 in Japan

Genetic diversity of circulating Saffold viruses in Pakistan and Afghanistan

Saffold cardioviruses in children with diarrhea

Saffold virus respiratory infection in children and immunocompromised patients in Spain

Prevalence and genetic characteristics of Saffold cardiovirus in China from 2009 to 2012

Three clusters of Saffold viruses circulating in children with diarrhea in Japan

SAffold viruses in pediatric patients with diarrhea in Thailand

Diversity of picornaviruses in rural Bolivia

Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis

Serious invasive Saffold virus infections in children

The preparation of an infectious fulllength cDNA clone of Saffold virus

Enteroviruses: Polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses

Pathologic characterization of a murine model of human enterovirus 71 encephalomyelitis

Coxsackievirus B3 and the neonatal CNS: the roles of stem cells, developing neurons, and apoptosis in infection, viral dissemination, and disease

A murine oral enterovirus 71 infection model with central nervous system involvement

A non-mouse-adapted enterovirus 71 (EV71) strain exhibits neurotropism, causing neurological manifestations in a novel mouse model of EV71 infection

Coxsackievirus-induced acute neonatal central nervous system disease model

Adaptation of Saffold virus 2 for high-titer growth in mammalian cells

Characterization of full genome of rat hepatitis E virus strain from Vietnam

The ddY mouse: a model of postprandial hypertriglyceridemia in response to dietary fat

Effects of Toll-Like Receptor Stimulation on Eosinophilic Infiltration in Lungs of BALB/c Mice Immunized with UV-Inactivated Severe Acute Respiratory Syndrome-Related Coronavirus Vaccine

Excessive cytokine response to rapid proliferation of highly pathogenic avian influenza viruses leads to fatal systemic capillary leakage in chickens

Synthetic double-stranded RNA poly(I:C) combined with mucosal vaccine protects against influenza virus infection

A novel real-time PCR system for simultaneous detection of human viruses in clinical samples from patients with uncertain diagnoses

Calbindin D28k-like immunoreactivity during the formation of the enamel-free area in the rat molar teeth

Spontaneous Encephalomyelitis of Mice, a New Virus Disease

Theiler's Murine Encephalomyelitis Virus (TMEV)-Induced Demyelination: A Model for Human Multiple Sclerosis

Pathogenesis of virus-induced immune-mediated demyelination

Pathogenesis of early and late disease in mice infected with Theiler's virus, using intratypic recombinant GDVII/DA viruses

A comparison of the neurotropism of Theiler's virus and poliovirus in CBA mice

Identification of a novel neuropathogenic Theiler's murine encephalomyelitis virus

Persistent infection of oligodendrocytes in Theiler's virusinduced encephalomyelitis

Saffold cardiovirus and multiple sclerosis: no evidence for an association

Adaptation of Theiler's virus to L929 cells: mutations in the putative receptor binding site on the capsid map to neutralization sites and modulate viral persistence

Sialylation of the host receptor may modulate entry of demyelinating persistent Theiler's virus

The leader protein of Theiler's virus inhibits immediate-early alpha/ beta interferon production

The leader protein of Theiler's virus interferes with nucleocytoplasmic trafficking of cellular proteins

Opposite effects of two nonstructural proteins of Theiler's murine encephalomyelitis virus regulates apoptotic cell death in BHK-21 cells

Apoptotic and antiapoptotic activity of L protein of Theiler's murine encephalomyelitis virus

Genomic regions of neurovirulence and attenuation in Theiler murine encephalomyelitis virus

Microdomains for neuron-glia interaction: parallel fiber signaling to Bergmann glial cells

Neuropathological findings in West Nile virus encephalitis: a case report

Fatal West Nile virus encephalitis in a renal transplant recipient

Visualization of Central European tick-borne encephalitis infection in fatal human cases

Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA

Differential localization of neurons susceptible to enterovirus 71 and poliovirus type 1 in the central nervous system of cynomolgus monkeys after intravenous inoculation

Establishment of a panel of in-house polyclonal antibodies for the diagnosis of enterovirus infections

We thank our colleagues at the National Institute of Infectious Diseases in Tokyo, Japan, particularly Ms. Ayako Harashima, Ms. Mihoko Fujino, and Ms. Moeko Aida, for technical assistance. We are also grateful to Dr. Tian-Cheng Li, Dr. Chikako Kataoka, and Dr. Tomofumi Nakamura for their technical expertise in the field of virus genome sequencing, to Dr. Harutaka Katano for the viral RNA real-time RT-PCR assays, to Dr. Akira Ainai for the mouse type 1 interferon real-time RT-PCR assays, and to Dr. Minoru Tobiume for histopathological analysis of the oral cavity. We also thank Dr. Shin-ichi Tamura, Dr. Hideki Asanuma, and Dr. Akira Ainai for valuable discussion.