key: cord-0001637-g6nvhdmv
authors: Wu, Guangming; Schöler, Hans R
title: Role of Oct4 in the early embryo development
date: 2014-04-29
journal: Cell Regen (Lond)
DOI: 10.1186/2045-9769-3-7
sha: 58246d246bb1b2288dc58245c475fc314d34cc83
doc_id: 1637
cord_uid: g6nvhdmv

Oct4 is a key component of the pluripotency regulatory network, and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells (ESCs) or their differentiation into trophoblast. Oct4 of maternal origin is postulated to play critical role in defining totipotency and inducing pluripotency during embryonic development. However, the genetic elimination of maternal Oct4 using a Cre-lox approach in mouse revealed that the establishment of totipotency in maternal Oct4–depleted embryos was not affected, and that these embryos could complete full-term development without any obvious defect. These results indicate that Oct4 is not essential for the initiation of pluripotency, in contrast to its critical role in maintaining pluripotency. This conclusion is further supported by the formation of Oct4-GFP– and Nanog- expressing inner cell masses (ICMs) in embryos with complete inactivation of both maternal and zygotic Oct4 expression and the reprogramming of fibroblasts into fully pluripotent cells by Oct4-deficient oocytes.

Life is like a journey of torch relay. From generation to generation, our bodies vanish at the end of our lives, but the germ cells are passed on to the next generation, ensuring the continuity and prosperity of our species. In comparison with the somatic cells, these germ cells possess many unique properties, of which the expression of Oct4 is the most important as it is required for the survival of primordial germ cells (PGCs) [1, 2] . Oct4 is also expressed specifically in the inner cell mass (ICM) and embryonic stem cells (ESCs), the cells derived from the ICM [3] . Interestingly, Oct4 is expressed in mouse oocytes as a maternal transcript and protein [1, [4] [5] [6] . As is typical for most maternal mRNAs, levels of Oct4 mRNA drop dramatically after fertilization [6] . Zygotic Oct4 expression is activated prior to the 8-cell stage, with a significant increase in both mRNA and protein levels [4, 6] . Oct4 expression is abundant and uniform in all cells of the embryo throughout the morula stage. However, as the outer cells of the embryo differentiate into the trophectoderm (TE), Oct4 expression becomes downregulated and restricted to cells of the ICM in the blastocyst [5, 7, 8] . When cells of the primitive endoderm differentiate and migrate away from the ectoderm, their Oct4 protein levels transiently increase [4] . Oct4 expression then becomes downregulated in the primitive endoderm and maintained in the epiblast, concurrently with embryo implantation and gastrulation. Oct4 expression finally becomes restricted to PGCs [9] , which are first specified in the extraembryonic mesoderm at the base of the allantoic bud during gastrulation [9] . PGCs give rise to gametes, which can be fertilized to develop into a new fully functional organism of the next generation and complete one cycle of life ( Figure 1 ).

Oct4, encoded by the gene Pou5f1, is a homeodomain transcription factor of the POU (Pit-Oct-Unc) family. The POU family of transcription factors can activate the expression of their target genes through binding to an octameric sequence motif of an ATGCAAAT consensus sequence. Oct4 protein consists of 3 domains: N-terminal domain, POU domain, and a C-terminal domain. The POU domain consists of two structurally independent subdomains: a 75 amino acid amino-terminal POUspecific (POU S ) region and a 60 amino-acid carboxylterminal homeodomain (POU HD ). Both domains make specific contact with DNA through a helix-turn-helix structure and are connected by a linker of 17 amino acids. Regions outside of the POU domain are not critical for DNA binding and exhibit little sequence conservation.

The N-terminal domain (N-domain) is rich in Proline and acidic residues, while the C-terminal domain (C-domain) is rich in Proline, Serine, and Threonine residues. Both the N-domain and the C-domain play an important role in transactivation, but the activity of the C-domain is cell type specific and is regulated through phosphorylation, whereas that of the N-domain is not. The Oct4 POUdomain functions differently by serving as an interaction site for binding by cell type-specific regulatory factors [12, 13] . Oct4 has been deemed to be a critical regulator of cellular pluripotency, as shown by a zygotic Oct4-knockout study [14] . Loss of pluripotency in embryos, observed at the onset of somitogenesis, is coincident with reduction of Oct4 and Nanog expression, and can be rescued by ectopic Oct4 expression [15] . Oct4 can activate its own expression with its transcription factor partner Sox2 through a positive autoregulatory loop in ESCs [16] . Studies on Oct4 interaction protein network have revealed that the Oct4 interactome includes many transcription factors and chromatin-modifying complexes with documented roles in self-renewal and pluripotency, and that acute depletion of Oct4 reduces the binding of Tcfcp2l1, Dax1, and Esrrb to target genes [17] [18] [19] . Depletion of Oct4 by siRNA leads to reduced binding of two key components of the bone morphogenetic protein (BMP) and leukemia inhibitory factor (LIF) signaling pathways, Smad1 and STAT3, to their respective targets. This result indicates that Oct4 plays a pivotal role in stabilizing the nucleoprotein complex and establishes a hierarchy of regulatory interactions between Oct4, STAT3, and Smad1 [20] . The core components of the pluripotency circuitry are formed by Oct4, Nanog, and Sox2, and Nanog expression is directly regulated by Oct4 and Sox2 [21] , Sox2 is actually dispensable for the activation of Oct-Sox enhancers, and the forced expression of Oct4 could rescue Sox2-null ESCs [22] . Hence, Oct4 is considered to be the genetic "master switch" in the establishment of totipotency-pluripotency during the life cycle of mammals [23] , and it is presumed to be the most upstream gene in the molecular circuitry of pluripotency [24] .

Maternal Oct4 expression is not critical for developmental competence of the oocyte All biological processes occurring during the first cell cycle of the embryo rely on maternal factors, which have accumulated during the long growth phase of the germinal vesicle (GV) oocyte [25] , because there is nearly no mRNA synthesis between the end of the mouse oocyte growth phase and the first zygotic cleavage [26] . But during the second cell cycle, there is a burst of transcription-what is known as zygotic genome activation (ZGA) [27] . Mature metaphase II (MII) oocytes have the capacity to reprogram somatic cells into cells of a totipotent state via nuclear transfer (NT) [28, 29] . Therefore, activation of zygotic transcripts mediated by maternal regulatory factors provides the first step in the establishment of totipotency/pluripotency. Oct4 is one of the 27 proven maternal-effect genes and is regarded to be functionally important for zygotic genome activation [30] . Maternal Oct4 is therefore widely accepted to play a role in igniting the establishment of totipotency and induction of pluripotency.

According to the chromatin organization, growing mouse oocytes could be classified into 2 types: SN (surrounded nucleolus) oocytes, with a ring of chromatin surrounding the nucleolus, and NSN (not surrounded nucleolus) oocytes, with chromatin dispersed throughout the nucleus, i.e., chromatin not surrounding the nucleolus. When they mature into metaphase II (MII) oocytes and are fertilized in vitro, only MII SN oocytes (MII oocytes derived from SN oocytes) are developmentally competent to develop beyond the 2-cell stage, while MII NSN oocytes (MII oocytes derived from NSN oocytes) However, as the outer cells of the embryo differentiate into the TE, Oct4 expression is restricted to cells of the ICM in the blastocyst. After implantation, Oct4 expression is maintained in the epiblast. Finally, Oct4 expression becomes restricted to primordial germ cells (PGCs), which are first specified in the extraembryonic mesoderm at the base of the allantoic bud during gastrulation. PGCs give rise to gametes, which following fertilization will develop into a new organism of next generation. Oct4 expression is based on data previously reported by several studies [6, 7, [9] [10] [11] .

become arrested at the 2-cell stage [31] . By comparing the transcriptional profiles of these two types of mouse MII oocytes, Zuccotti et al. found that Oct4 was absent in MII NSN oocytes, accounting for the downregulation of Stella, a maternal-effect factor required for the oocyteto-embryo transition, and the upregulation of 18 Oct4regulated genes implicated in the activation of adverse biochemical pathways such as oxidative phosphorylation, mitochondrial dysfunction, and apoptosis. Those authors concluded that the downregulation of Oct4 leads to the developmental arrest of MII NSN oocytes and that maternal Oct4 emerges as a key regulator of the molecular events that govern the establishment of the developmental competence of mouse oocytes [32] . Moreover, another study claimed that Oct4 is a critical regulator of the maternal-embryonic transition at the 2-cell stage, as embryos invasively injected with Oct4-antisense morpholino oligonucleotides were found to arrest in various developmental stages prior to the blastocyst stage [33] . A later report from the same group using the same approach also showed developmental arrest between the 2to 8-cell stages in morpholino-mediated Oct4, Nanog, or Sall4 knockdown, but developmental arrest remained obvious after co-injection of Oct4, Nanog, or Sall4 mRNA [34] . Although such a phenotype contradicts to genetic knockout studies [14, 35, 36] , which indicated that homozygous null embryos could develop at least up to the blastocyst stage, the authors ignored the presence of maternal Oct4 protein and assumed that the apparent discrepancy was due to the presence of functional maternal transcript [34] . However, recently, numerous studies found that after genetic removal of maternal Oct4 in oocytes by crossing Oct4 flox/flox /ZP3 Cre/+ female mice with wild-type male mice, these Oct4 flox/flox /ZP3 Cre/+ female mice were found to be fully fertile [37] [38] [39] . All offspring had deletion of the maternal Oct4 allele as confirmed by polymerase chain reaction (PCR) genotyping, clearly demonstrating that maternal Oct4 is not critical for the establishment of totipotency-pluripotency [37] . The data in the study also showed the efficient deletion of the maternal Oct4 allele by genotyping of individual oocytes, the depletion of Oct4 mRNA by real-time reverse transcriptase PCR (RT-PCR), and the depletion of Oct4 protein by Western blot [37] .

Enucleated oocytes can also reprogram the nuclei of terminally differentiated somatic cells to a totipotent embryonic state after nuclear transplantation [28, 29] . The nuclei can be reprogrammed by the recipient oocytes to express the pluripotent gene Oct4 at a very high efficiency (88.7%) in just 2-3 days [40] and to give rise to pluripotent cells at an efficiency of up to 20% [41] . This compares favorably with the exciting 4-factor (Oct4, Sox2, Klf4, and c-Myc) technique of generating induced pluripotent stem cells (iPSCs) by Yamanaka and colleagues, which showed activation of internal Oct4 expression after more than 2 weeks, with an efficiency of 0.01-0.1% [42] . A recent study revealed that Oct4 alone could reprogram neural stem cells into pluripotent iPSCs [43] . Could maternal Oct4 in oocytes be essential in the reprogramming of somatic cells to pluripotent status? The answer is no. By conducting NT experiments using maternal Oct4-null oocytes from Oct4 flox/flox /ZP3 Cre/+ female mice, it was found that all cloned embryos expressed pluripotency genes (Oct4 and Nanog) in the ICM and the TE marker gene Cdx2 in the TE by both immunocytochemistry and real-time RT-PCR [37] . Furthermore, ESC lines derived from cloned embryos using Oct4-knockout oocytes demonstrated full pluripotency by generating completely ESCderived mice through the tetraploid complementation test, the most stringent test for pluripotency [37] . Clearly, the reprogramming engine in oocytes could work effectively to reprogram somatic cells into cells of a pluripotent state without the presence of maternal Oct4.

Previous studies have suggested that lineage commitment is controlled by the expression level of Oct4 [14, 44] . Repression of Oct4 expression induced the differentiation of ESCs into TE cells, and a less than two-fold increase in Oct4 expression caused the differentiation of ESCs into cells of primitive endoderm and mesoderm [44] . In the absence of Oct4, embryos could not form an ICM-i.e., the inner cells of morula-stage embryos rather were driven into trophoblast differentiation [14] . Therefore, these results indicated that Oct4 plays a critical role in sustaining stem cell self-renewal and that up-or downregulation of Oct4 expression induces divergent developmental programs, suggesting that Oct4 is the master regulator of pluripotency and that it may also control lineage commitment during early embryonic development [14] . This hypothesis was supported by reports showing that Oct4 and Cdx2 could form a complex that reciprocally repressed their target genes and such an interaction determined the ICM/TE lineage separation in early embryos [45] , while the interaction between Oct4 and the histone H3-specific methyltransferase ESET restricted the extraembryonic trophoblast lineage potential of pluripotent cells [46] . However, the expression of Cdx2 in ESCs was found to be rapidly initiated by Ras activation (i.e., within 24 hours) without previous or simultaneous downregulation of Oct4 expression [47] , while ESCs with reduced Oct4 expression showed robust pluripotency and expressed naïve pluripotency genes, but were deficient in differentiation in the absence of pluripotency culture requisites [48, 49] . On the other hand, Cdx2 deficiency did not interrupt TE-ICM lineage separation [50] [51] [52] , and zygotic Oct4 expression was shown not to be required for the initial repression of the TE genes Cdx2 and Gata3 in the ICM, indicating that other mechanisms are responsible for restricting the expression of these genes to the TE [53] . Finally, with a conditional knockout system, it was demonstrated that, similar to the reported zygotic Oct4 knockout observation [14, 53] , the genetic removal of both maternal and zygotic Oct4 did not prevent ICM-TE lineage separation [37] [38] [39] , arguing against the notion that maternal Oct4 could partially compensate for the loss of zygotic Oct4 during cell fate specification in the blastocyst [33] . Therefore, the first lineage separation of the ICM-TE is not determined by the reciprocal interaction between Oct4 and Cdx2. More importantly, these studies indicated that maternal Oct4 is not at the root of pluripotency as a determinant of pluripotent cell lineage initiation, contrary to previous assumptions and views [23, 24] .

Oct4 is at the top of the pluripotency regulatory hierarchy in pluripotent cells [20, 21] . It forms a positive feedback loop [16] and is essential to maintaining pluripotency [14] , but is not required for initiating totipotency/pluripotency in embryos [37] [38] [39] . Therefore, understanding how early embryos activate Oct4 expression is key in clarifying the oocyte reprogramming mechanism.

The upstream region of the transcriptional initiation site of the Oct4 gene contains three regulatory elements for gene transcription: the distal enhancer (DE), proximal enhancer (PE), and TATA-less proximal promoter (PP) [9] as illustrated in Figure 2 . The two enhancers exhibit a differential activation pattern according to the developmental stage of the mouse embryo. The DE drives Oct4 expression in the ICM, ESCs, and PGCs, while the PE activates Oct4 expression in epiblast cells. Each enhancer contains multiple potential binding sites for transcription factors that can either activate or repress Oct4 expression. In addition, the methylation of these regions represses Oct4 expression in differentiated cells. Several positive and negative regulators bind to the Oct4 gene to regulate its expression. Of these, members of the orphan nuclear receptor superfamily, which can bind to Sp1 sites [54] and hormone response elements (HREs) in the PE and PP, are known to influence Oct4 expression. Positive regulators of Oct4 expression include Nr5a2 [55] , SF1 (Steroidogenic Factor-1), and RXR-β (Retinoid X Receptor-β, also known as Nr2b2) [56, 57] . Negative regulators include GCNF (Germ Cell Nuclear Factor) (also known as Nr6a1) [58] , and COUF-TFI/II (Chicken Ovalbumin Upstream promoter-Transcription Factors-I/II), encoded by Nr2f1 and Nr2f2, respectively [59, 60] . The transcription factor TR2 can bind to the HRE of the Oct4 gene to either activate or repress Oct4 expression in P19 embryonal carcinoma (EC) stem cells and regulate the proliferation of the culture, based on whether there is SUMOylation on the Lys-238 of TR2 [61] .

Recent studies have found that certain maternal factors are involved in the regulation of Oct4 expression, providing clues on the mechanism underlying the initiation of totipotency/pluripotency. Cancer-associated factor Tpt1 has been reported to activate the transcription of Oct4 and Nanog in transplanted somatic nuclei in the Xenopus oocyte [63] , but another study failed to replicate this finding upon knockdown of Tpt1 by Small interfering RNA (siRNA) in the mouse embryos [37] . Components of the ATP-dependent BAF chromatinremodeling complex have been shown to significantly increase reprogramming efficiency when used together with the Yamanaka's 4 factors [64] . Promyelocytic leukemia (Pml) protein was found to be required for Oct4 gene expression and the maintenance of its open chromatin conformation in stem cells. In proliferating stem cells, Pml-nuclear body, along with the transcription factors TR2, SF1 and Sp1, and the Brg1-dependent chromatin remodeling complex (BRGC), associates with the Oct4 promoter to maintain a nucleosome-free region for gene activity [65] . Studies in search for master genes in the oocyte have revealed a novel oocyte-specific eukaryotic translation initiation factor 4E (Eif4eloo) [66] and a large number of oocyte-specific genes with yet unknown functions, such as those belonging to the homeodomain transcription factor Obox family [67] . The maternal transcription factor Sall4 binds to the Oct4 DE, and it is regarded to be a transcriptional activator of Oct4 expression based on evidence that reduction in Sall4 mRNA level in blastocysts at merely 50% knockdown efficiency by Sall4 siRNA injection into zygotes led to a 70% reduction in Oct4 expression [68] . Singlecell expression analyses during in vitro cellular reprogramming have confirmed that Sall4 is indeed an upstream activator of Oct4 expression [69] . However, the efficient knockdown of Sall4 by injecting Sall4 siRNA into maternal Oct4-deficient zygotes-to avoid any possible effect of maternal Oct4 as a positive autoregulator-did not lead to any changes in Oct4 expression at the blastocyst stage, arguing against such a role for Sall4 as an upstream activator of Oct4 expression in vivo [37] . Nr5a2 was found to maintain Oct4 expression at the epiblast stage of embryonic development, by binding to the PE and PP regions of Oct4, but to play no evident role in ESC self-renewal [55] . However, Nr5a2 can induce epiblast stem cells into ground state pluripotency, a basal proliferative state that is free of epigenetic restriction [70] , and replace Oct4 in the reprogramming of somatic cells into pluripotent cells [71] . Activation of Zscan4 expression occurs during ZGA, with the gene being expressed in ESCs, whereas reduction in Zscan4 transcript levels by siRNAs delays the progression from the 2-cell to the 4-cell stage, leading to blastocysts that fail to implant or proliferate in blastocyst outgrowth culture [72] . Zscan4 is essential for induction of iPSCs and its ectopic expression can activate early embryonic genes and improve the efficiency of iPSC generation [73] . However, knockdown of Zscan4 in preimplantation embryos by siRNA against all 6 isoforms of Zscan4 (a-f ) had no impact on Oct4 expression [37] . A component of an active DNA demethylase, activation-induced cytidine deaminase (AID), was also shown to be required for reprogramming [74] . A genome-scale RNA interference (RNAi) screen in ESCs identified components of the Paf1 complex with strong effects on Oct4 expression, and showed that Paf1C overexpression blocks the differentiation of ESCs and that Paf1C knockdown causes expression changes in ESCs that are similar to those observed with Oct4 or Nanog depletion [75] .

After the finding that truncated isoforms of OCT4 are transcribed from the POU5F1 gene in human [76] and mouse [77] , the originally described OCT4 is designated as OCT4A and the newly found truncated isoforms are variants of an OCT4 version named OCT4B [78] . OCT4B mRNAs encode proteins that have identical POU DNA-binding domains and C-domains but differ in their N-domains (Figure 3 ). Continued expression of Oct4B after the original Oct4 promoter was removed indicates that Oct4B transcription is regulated by an alternative promoter in the first intron [37] , as presumed by an earlier study [79] . Other isoforms of Oct4B could be produced by alternative splicing or alternative translation initiation [80, 81] . ESC-based complementation assays using ZHBTc4 ESCs, which has endogenous Oct4 inactivated by gene targeting and harbors a tetracyclinerepressible Oct4 transgene to support ESC self-renewal [44] , showed that OCT4B cannot rescue the self-renewal ability of ZHBTc4 ESCs in the presence of doxycycline, unlike OCT4A. Electrophonetic mobility shift assay showed that OCT4B does not bind to a probe carrying the OCT4 consensus binding sequence due to the repressive effect of the OCT4B N-domain. Furthermore, overexpression of OCT4B does not activate transcription from OCT4-dependent promoters [78] . However, Oct4B is involved in stress response [82] and acts as an antiapoptotic factor in cancer cells [83] .

On the other hand, Oct4 expression in the adult has been reported in hematopoietic and mesenchymal stem cells [84] [85] [86] [87] [88] [89] [90] [91] [92] [93] [94] [95] , as well as progenitor cells from various somatic tissues including pancreatic islets [96] , kidney [97, 98] , peripheral blood [89, 99, 100] , endometrium of the uterus [101, 102] , thyroid [103] , lung [104] , brain [105, 106] , liver [107] , and skin [108] [109] [110] [111] . The wide expression of Oct4 in normal tissues suggests that Oct4 may not only be crucial for the maintenance of pluripotency in embryonic cells, but also play an important role in the self-renewal of somatic stem cells and in maintenance of tissue homeostasis. As these studies did not distinguish the Oct4 isoforms, it is likely they actually detected Oct4B. A later study that conditionally deleted Oct4 from somatic cells in vivo found that Oct4 is dispensable for both the self-renewal and maintenance of somatic stem cells in the adult mammal [112] . Oct4 gene ablation in the intestinal epithelium, bone marrow (hematopoietic and mesenchymal lineages), hair follicle, Figure 2 Genomic structure and transcriptional regulation of the mouse Oct4 gene. The diagram represents~24 kb of the genomic region surrounding the Oct4 gene [62] . The gene has five exons, depicted as blue boxes. The identified upstream regulatory regions include the promoter, proximal enhancer, and distal enhancer. The sizes of the regulatory elements are stretched to enhance clarity. The transcription factors bind to these regions, and are shown above within colored boxes; they either activate (green box) or repress (red box) transcription. HRE = hormone responsive element; Sp1 = GC-rich site recognized by the Sp1/Sp3 family of transcription factors. CR1, CR2, CR3, and CR4 are conserved regions (CRs) at the 5' upstream region of the Oct4 gene.

brain, and liver revealed no abnormalities in homeostasis or regenerative capacity [112] . Like many other publications claiming Oct4 expression in somatic stem cells, this study also noted low level of expression but that was regarded as false detection of Oct4 due to the noise of the detection methods, expression of pseudogenes, and expression of other POU-domain family members [112] . These conclusions are now in question. In our lab, the same Oct4floxed mice were used, in which two LoxP motifs had been inserted that span the proximal promoter and the Oct4Aunique first exon [2] . As the other 4 exons shared by Oct4B were not mutated, it is still possible that Oct4B can still be transcribed using an alternative promoter. After efficiently removing the floxed sequence of Oct4 in oocytes by crossing the Oct4 flox/flox mice with ZP3Cre transgenic mice, indeed, the expression of only Oct4B (not Oct4A) was detected at low levels by RT-PCR in the mutated oocytes and preimplantation embryos, which was confirmed by sequencing. Another recent report used a different Oct4floxed mouse line to delete the entire Oct4 POU domain and C-domain, yet the authors of that study also observed full-term development of maternal Oct4-null embryos and TE/ICM lineage separation, as well as Nanog activation in maternal and zygotic Oct4-null embryos [39] . Taken together, these studies confirmed that (1) maternal Oct4 is indeed not essential for the establishment of totipotency. (2) The low levels of Oct4B expression could not rescue Oct4A-null embryos to maintain pluripotency in vivo [37, 38] . The precise function of Oct4B in embryos and somatic stem cells remains to be clarified.

As summarized in Figure 4 , new pieces of evidence clearly indicate that Oct4 is not the master regulator responsible for initiating totipotency-pluripotency in oocytes, and that maternal and zygotic Oct4-null blastocysts maintain the ability to activate Nanog and Oct4-GFP expression, indicating that unknown pathways other than the Oct4-centered pluripotency-regulating network are active in embryos and function upstream of Oct4 in driving pluripotency. However, to date no factors have proven to be essential for Oct4 activation in the preimplantation embryos. Further studies are required to elucidate how oocytes activate the pluripotent genes Oct4 and Nanog on top of the Oct4/Sox2 autoregulatory loop in an effort to understand the establishment of totipotency in zygotes and in transplanted somatic cells. 

The authors declare that they have no competing interests.

Authors' contributions GW drafted the manuscript. HRS and GW outlined, edited and revised the manuscript and all authors read and approved the final manuscript.

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Cite this article as: Wu and Schöler: Role of Oct4 in the early embryo development

This research was supported by the Max Planck Society, DFG grant SI 1695/ 1-2 (SPP1356) and NIH grant R01HD059946-01 from the Eunice Kennedy Shriver National Institute of Child Health & Human Development. We thank Areti Malapetsas for final editing. 

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