key: cord-0001477-yt9cldeb authors: Wang, Jianning; Selleck, Paul; Yu, Meng; Ha, Wendy; Rootes, Chrissy; Gales, Rosemary; Wise, Terry; Crameri, Sandra; Chen, Honglei; Broz, Ivano; Hyatt, Alex; Woods, Rupert; Meehan, Brian; McCullough, Sam; Wang, Lin-Fa title: Novel Phlebovirus with Zoonotic Potential Isolated from Ticks, Australia date: 2014-06-03 journal: Emerg Infect Dis DOI: 10.3201/eid2006.140003 sha: b333e890955fbc28fc246b5e97467a0312f4def3 doc_id: 1477 cord_uid: yt9cldeb Recently discovered tick-borne phleboviruses have been associated with severe disease and death among persons in Asia and the United States. We report the discovery of a novel tick phlebovirus in Tasmania State, Australia, that is closely related to those zoonotic viruses found in Asia and North America. hospitalized for fever, fatigue, diarrhea, thrombocytopenia, and leukopenia. Both men had been bitten by ticks 5-7 days before the onset of symptoms. A virus was isolated from the leukocytes of each patient and later identified as a novel phlebovirus by next-generation sequencing. The 2 viruses were highly related (98%, 95%, and 99% sequence identity for the small, medium, and large viral genome segments, respectively), indicating that the men were independently infected with the same virus. This new virus, named Heartland virus (HRTV), was most closely related to, but clearly distinct from, the SFTSV detected in China (7) . Subsequent to these disease events in China and the United States, fatal SFTSV infections were reported in humans in Japan (8) and Korea (9) . Because similar viruses and human infections have been detected in 2 well-separated continents (i.e., Asia and North America), it is tempting to hypothesize that similar viruses may also exist in tick populations on other continents. We report the isolation and characterization of a phlebovirus from ticks in Australia that is similar to SFTSV and HRTV. In 2002, a disease outbreak occurred in a shy albatross (Thalassarche cauta) colony on Albatross Island, a small island in the Hunter Island Group in northwestern Tasmania, Australia. The disease in the albatrosses was characterized by weight loss and death. Serum samples and ticks (Ixodes eudyptidis) from healthy and affected birds were sent to the CSIRO (the Commonwealth Scientific and Industrial Research Organization) Australian Animal Health Laboratory in Geelong, Victoria, Australia, for diagnostic investigation. Several laboratory tests were conducted to detect potential viral pathogens involved in the disease event. The ELISA or hemagglutination inhibition assay was used to test serum samples (N = 38) for antibodies against avian influenza virus, infectious bursal disease virus, fowlpox virus, and Newcastle disease virus. Neutralizing antibodies for Newcastle disease virus (titer of 64) were detected in 1 serum sample; otherwise, no antibodies against any of these suspected agents were detected. Pooled tick homogenates (N = 5) were cultured on chicken embryos, chicken embryo fibroblast and skin cells, and Vero cells. No virus was detected in chicken embryos or in any of the chicken cell lines used. In contrast, cytopathic effect was observed on the third passage in Vero cell cultures inoculated with 2 tick pools (1 collected from diseased birds and the other from healthy birds). Electron microscopy examination revealed morphologically similar viral particles from both cultures; the morphologic features resembled those of bunyaviruses ( Figure 1 ). However, testing with antibodies against a range of known bunyaviruses (orthobunyavirus, phlebovirus, and nairovirus) did not reveal any cross-reactivity. PCR analysis using bunyavirus-specific primers (10) also failed to detect any specific sequence from this newly isolated virus. The investigation was put on hold until 2011 when the Australian Animal Health Laboratory established its next-generation sequencing capability. RNA extracted from partially purified virus grown in Vero cells was used for random PCR amplification. The resulting products were used for library preparation and subsequently sequenced by using the 454 GS FLX system (454 Life Sciences, Branford, CT, USA) according to standard protocols (11, 12) . In total, 6.9 Mb of sequence, consisting of 23,617 reads, was obtained and assembled into 722 contigs. BLASTN (http://blast.ncbi.nlm.nih.gov/Blast.cgi) analysis at the nucleotide level showed no sequence identity to any of the known viruses in GenBank (data not shown). However, a protein-based BLASTX (http://blast.ncbi.nlm.nih. gov/Blast.cgi) search yielded 10 contigs showing protein sequence identity to SFTSV and some degree of sequence identity to several other members of the family Bunyaviridae. The virus was tentatively named Hunter Island Group virus (HIGV) after the location where the tick samples were obtained. On the basis of the partial sequences derived from next-generation sequencing, primers were designed to obtain the rest of the genome sequences by using gap-filling PCR and 5′ and 3′ RACE methods previously developed by our group (13) . The complete genome sequences of the large, medium, and small segments have been deposited in GenBank under accession numbers KF848980, KF848981, and KF848982, respectively. The Table summarizes the genetic and sequence features of the genome segments and deduced proteins of HIGV and several selected phleboviruses. From the sequence comparison, it is evident that HIGV is a new member of the genus Phlebovirus and that it is most closely related to the newly discovered zoonotic members of the genus, SFTSV and HRTV. This finding was further confirmed by phylogenetic analysis based on nucleotide and protein sequences of all 3 genome segments (representative trees shown in Figure 2 ). Because HIGV was isolated from ticks collected from healthy and diseased birds we believed it was unlikely that the virus was the causative agent for the disease in the shy albatrosses. To further confirm this, we conducted ELISA and Western blot analysis using a recombinant HIGV nucleocapsid protein, which was expressed and purified from Escherichia coli by using previously described methods (14, 15) . Of the 38 serum samples tested, none produced positive readings in ELISA or Western blot. In addition, none were positive when tested by HIGV-specific quantitative PCR targeting the polymerase gene of the large segment. We identified a novel tick-borne phlebovirus, HIGV, during the investigation of a disease outbreak among shy albatrosses in Tasmania. Genetic characterization showed that the virus is closely related to 2 newly discovered tickborne zoonotic phleboviruses (SFTSV and HRTV) that were responsible for severe disease and death in humans in 4 separate countries in Asia and North America. However, with the current data alone, the particular disease event in the shy albatrosses could not be attributed to HIGV. The findings from this study demonstrate the key role that a vigilant pathogen investigation has in any diagnostic assessment. The study findings also suggests that zoonotic phleboviruses genetically related to SFTSV, HRTV, and HIGV may be widely distributed in different parts of the world and that heightened international surveillance is needed to fully understand and appreciate the public health risk from these emerging viruses. Philadelphia: Lippincott Williams & Wilkins Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses Phylogenetic trees of recently isolated bunyaviruses based on amino acid sequences of the polymerase protein (A) encoded by the large segment, the membrane glycoprotein polyprotein (B) encoded by the medium segment, and the nucleocapsid protein (C) and the nonstructural protein (D) encoded by the small segment of selected bunyaviruses. Maximum-likelihood trees were constructed by using MEGA5 (www.megasoftware.net/) with bootstrapping at 1,000 replicates Molecular biology of phleboviruses Fever with thrombocytopenia associated with a novel bunyavirus in China An emerging hemorrhagic fever in China caused by a novel bunyavirus SFTSV Person-to-person transmission of severe fever with thrombocytopenia syndrome virus. Vector Borne Zoonotic Dis A new phlebovirus associated with severe febrile illness in Missouri The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan Severe fever with thrombocytopenia syndrome Consensus amplification and novel multiplex sequencing method for S segment species identification of 47 viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus genera of the family Bunyaviridae Field guide to next-generation DNA sequencers A novel bat herpesvirus encodes homologues of major histocompatibility complex classes I and II, C-type lectin, and a unique family of immune-related genes Improved rapid amplification of cDNA ends (RACE) for mapping both the 5′ and 3′ terminal sequences of paramyxovirus genomes Expression of equine morbillivirus (EMV) matrix and fusion proteins and their evaluation as diagnostic reagents Determination and application of immunodominant regions of SARS coronavirus spike and nucleocapsid proteins recognized by sera from different animal species We thank Richmond Loh for providing diagnostic samples, Ian Beveridge for help with tick species identification, and Mary Tachedjian and Volker Haring for technical advice on next-generation sequencing data analysis.Dr Wang is a research scientist in charge of the molecular diagnostics group at CSIRO Australian Animal Health Laboratory. His research interests include novel molecular diagnostics and pathogen discovery.