key: cord-0000870-6a5gr8mc
authors: Chang, Hui-Wen; Egberink, Herman F.; Rottier, Peter J.M.
title: Sequence Analysis of Feline Coronaviruses and the Circulating Virulent/Avirulent Theory
date: 2011-04-03
journal: Emerg Infect Dis
DOI: 10.3201/eid1704.102027
sha: eac2d30bfd0231911f3879b0d57be63297e4f351
doc_id: 870
cord_uid: 6a5gr8mc

nan

segregation. Hence, our data do not confirm the diagnostic potential of the M protein sequence nor do they support the suggested role of the membrane protein in FIP pathogenesis (9) .

Informative as it may be, comparative sequence analysis will eventually not be sufficient to answer the FECV/FIPV question. What will be needed is a reverse genetics system to generate and manipulate the FCoV genome as well as a cell culture system to propagate the viruses, both of which have thus far not been achieved. 

Feline infectious peritonitis. ABCD guidelines on prevention and management

Feline infectious peritonitis

Feline coronaviruses: a tale of two-faced types

A review of feline infectious peritonitis virus infection: 1963-2008

The molecular genetics of feline coronaviruses: comparative sequence analysis of the ORF7a/7b transcription unit of different biotypes

Two related strains of feline infectious peritonitis virus isolated from immunocompromised cats infected with a feline enteric coronavirus

Feline infectious peritonitis viruses arise by mutation from endemic feline enteric coronaviruses

Genetics and pathogenesis of feline infectious peritonitis virus

Feline infectious peritonitis: insights into feline coronavirus pathobiogenesis and epidemiology based on genetic analysis of the viral 3c gene

Significance of coronavirus mutants in feces and diseased tissues of cats suffering from feline infectious peritonitis

Virology Division, Department of Infectious Diseases &

Yalelaan 1, 3584 CL Utrecht, the Netherlands; email: p.rottier@uu.nl was used to generate a rooted neighbor-joining tree with the M gene sequence of canine coronavirus strain NJ17 (Genbank accession no. AY704917) as outgroup. Bootstrap confidence values (percentages of 1,000 replicates) are indicated at the relevant branching points

Viruses detected in cattery animals are indicated by a cattery designation after the virus. identification B) Alignment of amino acid sequences of partial M proteins of the FCoVs from panel A, as compared with a feline infectious peritonitis virus (FIPV) reference sequence (top line

The 5 aa residues at positions 108, 120, 138, 163, and 199, suggested by Brown and others (9) as potential diagnostic sites

We thank the veterinarians and cat owners in the Netherlands who provided FIP cases and fecal material for this study. We also thank Raoul de Groot for his constructive remarks on the manuscript.

Author affiliation: Utrecht University, Utrecht, The Netherlands