key: cord-0000455-zpk3gjwo
authors: Li, Zhenghe; Pogany, Judit; Tupman, Steven; Esposito, Anthony M.; Kinzy, Terri Goss; Nagy, Peter D.
title: Translation Elongation Factor 1A Facilitates the Assembly of the Tombusvirus Replicase and Stimulates Minus-Strand Synthesis
date: 2010-11-04
journal: PLoS Pathog
DOI: 10.1371/journal.ppat.1001175
sha: 80ae2988547a9163342117a914b53b6a63487901
doc_id: 455
cord_uid: zpk3gjwo

Replication of plus-strand RNA viruses depends on host factors that are recruited into viral replicase complexes. Previous studies showed that eukaryotic translation elongation factor (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex. Using a random library of eEF1A mutants, we identified one mutant that decreased and three mutants that increased Tomato bushy stunt virus (TBSV) replication in a yeast model host. Additional in vitro assays with whole cell extracts prepared from yeast strains expressing the eEF1A mutants demonstrated several functions for eEF1A in TBSV replication: facilitating the recruitment of the viral RNA template into the replicase complex; the assembly of the viral replicase complex; and enhancement of the minus-strand synthesis by promoting the initiation step. These roles for eEF1A are separate from its canonical role in host and viral protein translation, emphasizing critical functions for this abundant cellular protein during TBSV replication.

Genome-wide screens for host factors affecting RNA virus infections have led to the identification of several hundreds host proteins in recent years [1, 2, 3, 4, 5, 6, 7] . These works demonstrated complex interactions between the host and plus-stranded (+)RNA viruses, the largest group among viruses. (+)RNA viruses contain relatively small genomes and greatly depend on the resources of the infected hosts in many steps during the infection process. These viruses recruit numerous host proteins to facilitate their replication and spread [8, 9, 10] . Many host RNA-binding proteins have been implicated in replication of (+)RNA viruses, including ribosomal proteins, translation factors and RNA-modifying enzymes [8, 9, 10, 11, 12, 13, 14] . In spite of the extensive effort, the actual function of host factors in (+)RNA virus replication is known only for a small number of host factors [8, 10, 15, 16, 17] .

Tomato bushy stunt virus (TBSV) and other tombusviruses are model plant RNA viruses with 4.8 kb genomic (g)RNA coding for two replication proteins, termed p33 and p92 pol , and three proteins involved in cell-to-cell movement, encapsidation, and suppression of gene silencing [18, 19] . Yeast (Saccharomyces cerevisiae) expressing p33 and p92 pol replication proteins can efficiently replicate a short TBSV-derived replicon (rep)RNA [20, 21] . The tombusviral repRNA plays several functions, including serving as a template for replication and as a platform for the assembly of the viral replicase complex [19, 22, 23] . The viral RNA also participates in RNA recombination [6, 18, 24] , which likely plays a major role in virus evolution.

One of the major advantages of studying TBSV replication is the availability of genomic and proteomic datasets on virus-host interactions [4, 5, 6, 7, 10, 15, 25, 26, 27] . For example, systematic genome-wide screens of yeast genes have revealed that TBSV repRNA replication is affected by over 100 different host genes [5, 7] . Additional genome-wide screens with TBSV also identified ,30 host genes affecting TBSV RNA recombination [4, 6, 28] . The identified host genes code for proteins involved in various cellular processes, such as translation, RNA metabolism, protein modifications and intracellular transport or membrane modifications [3, 5, 7] .

Additional global approaches based on the yeast proteome microarray (protein array) have led to the identification of over 100 host proteins that interact with viral RNA or the viral replication proteins [25, 26] . Also, proteomics approaches with the highly purified tombusvirus replicase has determined at least seven proteins in the complex, including the viral p33 and p92 pol , the heat shock protein 70 chaperones (Hsp70, Ssa1/2p in yeast), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, encoded by TDH2 and TDH3 in yeast), pyruvate decarboxylase (Pdc1p), Cdc34p ubiquitin conjugating enzyme [14, 26, 27] and eukaryotic translation elongation factor 1A (eEF1A) [25] . The functions of GAPDH and Hsp70 have been studied in some detail [14, 29, 30, 31] , but the roles of the other host proteins, such as eEF1A, in the replicase complex are currently undefined. eEF1A is a highly abundant cellular protein with a role in delivering aminoacyl-tRNA to the elongating ribosome in a GTPdependent manner. Many additional functions have been ascribed to eEF1A including quality control of newly produced proteins, ubiquitin-dependent protein degradation, and organization of the actin cytoskeleton [32, 33] . Although eEF1A has been shown to be part of replicase complexes of several RNA viruses [16, 34, 35, 36] , studies on determining its functions in virus replication are hindered by several major difficulties. These include (i) genetic redundancy: yeast has two eEF1A genes (TEF1 and TEF2), whereas animals and plants have 2-7 genes and several isoforms of eEF1A. (ii) eEF1A provides essential functions for cell viability and mutations could have pleiotropic effects on protein translation, actin bundling and apoptosis. (iii) eEF1A is a very abundant protein that constitutes 1-5% of total cellular proteins, making it difficult to completely remove eEF1A from biochemical assays using cell extracts. (iv) eEF1A is also required for the translation of viral proteins in infected cells, making it difficult to separate its effect on translation versus replication, processes that are interdependent.

The first evidence that translation elongation factors, such as EF-Tu and EF-Ts, play a role in (+)RNA virus replication was obtained with bacteriophage Qbeta [34] . The eukaryotic homolog of EF-Tu, eEF1A was found to bind to many viral RNAs, including the 39-UTR of Turnip yellow mosaic virus (TYMV) [37] , West Nile virus (WNV), Dengue virus, Tobacco mosaic virus (TMV) and Turnip mosaic virus (+)RNA [35, 38, 39, 40] . In addition, eEF1A has also been shown to interact with various viral replication proteins or the replicases, such as the NS5A replication protein of Bovine viral diarrhea virus (BVDV) [41] , NS4A of hepatitis C virus (HCV) [42] , the TMV replicase [43] , and the Gag polyprotein of HIV-1 [44] . It is also part of the replicase complex of vesicular stomatitis virus, a negative-stranded RNA virus [45] .

The actual biochemical functions provided by eEF1A for (+)RNA virus replication are currently poorly understood. In case of WNV, eEF1A is co-localized with the WNV replicase in the infected cells and mutations in the WNV (+)RNA within the mapped eEF1A binding site have led to decreased minus-strand synthesis [46] . On the contrary, eEF1A was shown to enhance translation but repressed minus-strand synthesis of TYMV in vitro [37, 47, 48] . Overall, eEF1A likely plays a role in the replication of many RNA viruses. The interactions of eEF1A with viral RNAs and viral replication proteins and its high abundance in cells might facilitate recruitment of eEF1A into virus replication. eEF1A has been shown to interact with the components of the tombusvirus replicase, including the 39-UTR of the repRNA, as well as the p33 and p92 pol replication proteins [25] . eEF1A is also known to interact with the yeast Tdh2p (GAPDH) [49] , which is also a component of the tombusvirus replicase. Overall, the multiple interactions of eEF1A with various components of the tombusvirus replicase could be important for eEF1A to regulate yet unknown functions of the viral replicase complex.

In this paper, we characterized the functions of eEF1A in TBSV replication based on identification of functional eEF1A mutants in yeast as well as using in vitro approaches. The obtained data support the model that eEF1A plays several roles during TBSV replication, including facilitating the assembly of the viral replicase complex. Moreover, using in vitro replication assays, we demonstrate that eEF1A enhances minus-strand synthesis via stimulating the initiation step of the viral RNA-dependent RNA polymerase. Since eEF1A is also associated with several other viral replication proteins or binds to viral RNAs, it is possible that the uncovered functions of eEF1A might be utilized by other RNA viruses during their replication as well.

To determine the functions of eEF1A during tombusvirus replication, we generated ,6,000 yeast strains expressing eEF1A with random mutations (see Fig. S1A ) and tested the level of TBSV repRNA accumulation in a high-throughput assay [50] . In this assay, we used yeast strains, in which the two wt eEF1A genes (TEF1/TEF2) were deleted from the chromosome, while the wt or mutated eEF1A was expressed from plasmids. Importantly, a given eEF1A mutant is the only source of eEF1A in the yeast cells used. Using the high-throughput assay, we identified one yeast strain (N21) expressing an eEF1A mutant that supported reduced TBSV repRNA replication, while the other three strains with eEF1A mutants (named C42, C53 and C62) showed increased level of repRNA accumulation ( Fig. 1A and S1B-G). Interestingly, the eEF1A mutants supporting increased steady-state level of repRNA accumulation did not increase the relative level of p33 and p92 pol replication proteins (Fig. 1A , bottom panel; S1D-E). Thus, these eEF1A mutants likely affect TBSV replication directly. Accordingly, affinity purification of the solubilized tombusvirus replicase complex from yeast cells, followed by in vitro replicase activity assay revealed that the replicase from C42, C53 and C62 mutant eEF1A-expressing yeast strains had ,2-fold increased activities when compared with wt eEF1A-expressing yeast strain (Fig. 1B , lanes 1-6 versus 7-8). The amounts of replication protein p33 and the co-purified eEF1A were comparable in the purified replicase samples (Fig. 1B, bottom panel) , indicating that the differences in replicase activities in the mutants are likely due to enhanced replicase functions, and not due to altered proteins levels in the replicase complexes. Testing the ability of C42, C53 and C62 mutant eEF1As to bind to the viral RNA or to the p33 and p92 pol replication proteins in vitro ( Fig. S2B -C) did not reveal significant differences between the mutants and the WT. This further supports that these eEF1A mutations likely increase the function of the viral replicase without altering the protein and RNA components in the replicase.

Placing the identified mutations in the three novel gain-offunction mutants of eEF1A (V 301 D, L 374 V/N 377 K, and F 413 L; Fig. 1C , indicated with yellow balls), which exhibited increased tombusvirus replication, over the known structure of eEF1A [51] revealed a cluster on one face of eEF1A (namely, the actin bundling domain III), away from the domains known to bind to tRNA and translation factor eEF1Ba. On the other hand, the new reduced function mutant (A 76 V, Fig. 1C , indicated with green balls) and the previously identified T 22 S [25] , which exhibited

Plus-stranded RNA viruses are important pathogens of plants, animals and humans. They replicate in the infected cells by assembling viral replicase complexes consisting of viral-and host-coded proteins. In this paper, we show that the eukaryotic translation elongation factor (eEF1A), which is one of the resident host proteins in the highly purified tombusvirus replicase complex, is important for Tomato bushy stunt virus (TBSV) replication in a yeast model host. Based on a random library of eEF1A mutants, we identified eEF1A mutants that either decreased or increased TBSV replication. In vitro studies revealed that eEF1A facilitated the recruitment of the viral RNA template for replication and the assembly of the viral replicase complex, as well as eEF1A enhanced viral RNA synthesis in vitro. Altogether, this study demonstrates that eEF1A has several functions during TBSV replication. eEF1A mutants enhance TBSV RNA replication in a cellfree extract

Since eEF1A is part of the tombusvirus replicase complex [25] , it is possible that C42, C53 and C62 eEF1A mutants might affect the assembly/activity of the tombusvirus replicase. To test this idea, we prepared cell-free extracts (CFE) from yeast strains expressing selected eEF1A mutants in the absence of the wt copy of eEF1A. These yeast extracts contained comparable amount of total proteins as well as the amounts of eEF1A, ALP, PGK and Hsp70 (Ssa) yeast proteins were comparable ( Fig. 2A) . The advantage of the CFE extracts is that they can then be programmed with the TBSV (+)repRNA in the presence of purified recombinant p33 and p92 pol obtained from E. coli that leads to the in vitro assembly of the viral replicase, followed by a single cycle of complete TBSV replication, resulting in both (2)stranded repRNA and (+)-stranded progeny [31, 52] . Therefore, this assay can uncouple the translation of the viral proteins from viral replication, which are interdependent during (+)RNA virus infections.

Using CFEs from yeast expressing one of the three mutant eEF1As resulted in ,3-fold increased TBSV repRNA accumulation when compared with the extract obtained from yeast expressing the wt copy of eEF1A ( Fig. 2A , lanes 2-4 versus 5). These data suggest that the viral replicase complex containing the mutant eEF1A can support in vitro TBSV repRNA replication more efficiently than the replicase with the wt eEF1A. In contrast, CFE from N21 yeast supported TBSV repRNA replication to similar extent as the CFE containing wt eEF1A ( Fig. 2A , lanes 1 versus 5), indicating that N21 eEF1A mutant can perform the same functions as the wt eEF1A in vitro, when the same amount of p33 and p92 pol was provided.

To test if the increased TBSV repRNA replication in vitro was due to enhanced (+) or (2)-strand synthesis, we analyzed the replication products under non-denaturing versus denaturing conditions (Fig. 2B) . These experiments showed that the amount of dsRNA [representing the 32 P-labeled (2)RNA product hybridized with the (+)RNA] increased ,3-fold in case of C42, C53 and C62 mutants (lanes 3-8, Fig. 2B ) in comparison with the wt (lanes 9-10). The dsRNA nature of these products was confirmed by the ssRNA-specific S1 nuclease digestion assay (Fig. 2C ). On the other hand, the ratio of dsRNA and ssRNA did not change in the various CFEs containing the eEF1A mutants or the wt (Fig. 2B) . These results are consistent with the model that the replicase complex carrying the eEF1A mutants increased mostly the level of (2)RNA production, which then led to proportionately higher level of (+)RNA progeny.

Cell-fractionation assay, followed by the cell-free TBSV replication assay demonstrated that the soluble fraction from the C42, C53 and C62 mutant yeasts stimulated the in vitro replication of TBSV repRNA by ,3-fold, while the membrane fraction when derived from C42, C53 and C62 mutant yeasts had a lesser effect (Fig. 2D , lanes 12-14 versus 7-9). These data are in agreement with the expected mostly cytosolic distribution of eEF1A, albeit eEF1A is also present in the membrane fraction in a smaller amount (Fig. 2D , bottom panel).

To test directly if eEF1A could stimulate RNA synthesis by the viral RdRp, we chose the E. coli-expressed recombinant p88 pol RdRp protein of Turnip crinkle virus (TCV), which is unlike the E. coli-expressed TBSV or CNV p92 pol RdRp, does not need the yeast cell-free extract to be functional in vitro [53, 54] . The template specificity of the recombinant TCV RdRp with TBSV RNAs is similar to the closely-related tombusvirus replicase obtained from yeast or infected plants [21, 54, 55, 56] . However, the recombinant TCV RdRp preparation lacks co-purified eEF1A, unlike the yeast or plant-derived tombusvirus replicase preparations, facilitating studies on the role of eEF1A on the template activity of a viral RdRp. When we added the highly purified wt eEF1A to the RdRp assay containing TCV RdRp protein and a TBSV derived (+)RNA template, which is used by the TCV RdRp in vitro to produce the complementary (2)RNA product (Fig. 3A , lanes 3-4) [55] , we observed a ,6-fold increase in (2)RNA synthesis by the TCV RdRp (lanes 11-12), while, as expected, we did not detect new (+)RNA progeny (not shown). This suggests that eEF1A can greatly stimulate TCV RdRp activity in vitro, confirming a direct role for eEF1A in (2)RNA synthesis by a viral RdRp.

Since it is known that eEF1A can bind to the 39-UTR of TBSV (+)RNA as well as to the tombusvirus replication proteins [25] and to the TCV RdRp ( Fig. S2A) , we wanted to test if the above stimulating activity of eEF1A in the in vitro RdRp assay was due to binding of eEF1A to the (+)RNA template and/or to the TCV RdRp protein.

Pre-incubation of the purified wt eEF1A with the TCV RdRp prior to the RdRp assay led to a ,5-fold increase in in vitro (2)RNA synthesis (Fig. 3A , lanes 9-10), while pre-incubation of the purified eEF1A with the TBSV (+)RNA template prior to the RdRp assay led only to a ,2-fold increase in (2)RNA products (lanes 7-8). Also, preincubation of the TCV RdRp with the (+)RNA template prior to the RdRp assay containing purified eEF1A led only to a ,2-fold increase in (2)RNA synthesis (lanes 5-6), suggesting that eEF1A can stimulate (2)RNA synthesis less efficiently after the formation of the (+)RNA-RdRp complex. Overall, data shown in Fig. 3 imply that eEF1A stimulates (2)RNA synthesis most efficiently when it forms a complex with the viral RdRp prior to binding of the template RNA to the eEF1A-RdRp complex.

To test if eEF1A stimulates the rate of initiation of (2)RNA synthesis, we analyzed the amount of abortive RNA products, which are generated during de novo initiation of RNA synthesis by the TCV RdRp [57] . We found that the amount of the 5-11 nt long abortive RNA products increased by 3.5-fold in the presence of purified eEF1A in the TCV RdRp assay (Fig. 3B , lanes 3-4 versus 1-2). We also tested the RdRp activity in the presence of Top panel: Replication of the TBSV repRNA was measured by Northern blotting 24 h after initiation of TBSV replication. The accumulation level of repRNA was normalized based on the rRNA (middle panel, the 18S ribosomal RNA levels were estimated by Northern blotting). Bottom two panels: Accumulation of p33/p92 pol and eEF1A was estimated by Western blotting using anti-His and anti-eEF1A antibody, respectively. Note that * marks an SDS-resistant p33 homodimer band. (B) An in vitro replicase assay to test the relative activity of the tombusvirus replicase obtained from yeast expressing various mutants of eEF1A. Top panel: We tested the in vitro replicase activity using comparable amounts of affinity-purified replicase with added DI-72 RI(2) RNA template. Bottom panels: Western blot analysis showing p33 viral replication protein and the co-purified eEF1A in the above purified replicase preparations. (C) Critical eEF1A residues for tombusvirus replication. Three novel mutants of eEF1A were identified, which exhibited increased tombusvirus replication (V301D, L374V/N377K, and F413L; yellow balls) while the new A76V and the previously identified T22S exhibited decreased tombusvirus replication (green balls). The structure of eEF1A was generated using Jmol with PDB coordinates 1IJE. doi:10.1371/journal.ppat.1001175.g001 eEF1A using a (+)RNA template with a mutation opening the closed structure in the promoter region that leads to increased template activity [58] . The mutated template also showed 2-fold increased abortive RNA products in the RdRp assay with eEF1A ( Fig. 3B , lanes 5 versus 6). These data strongly support the model that eEF1A stimulates the de novo initiation step in the RdRp assay. The denaturing PAGE analysis of the 32 P-labeled repRNA products obtained is shown. The full-length repRNA is pointed at by an arrow. Panels below show Western blot analysis of the whole cell extracts for the indicated yeast proteins based on specific antibodies. Bottom panel shows the coomassie-blue stained SDS-PAGE gel to visualize total protein levels in the whole cell extracts. (B) Detection of single-and double-stranded RNA products produced in the cell-free TBSV replicase assay. Odd numbered lanes represent replicase products, which were not heat treated (thus both ssRNA and dsRNA products are present), while the even numbered lanes show the heat-treated replicase products (mostly ssRNA is present). The amount of dsRNA and the ratio of ssRNA/dsRNA in the samples are shown. Note that, in the nondenatured samples, the dsRNA product represents the annealed (2)RNA and the (+)RNA, while the ssRNA products represents the newly made (+)RNA products. (C) Denaturing PAGE analysis of the TBSV replicase products obtained in the cell-free replicase assay after S1 nuclease treatment, which cleaves the ssRNA, but not the dsRNA product. (D) The denaturing PAGE analysis of the 32 P-labeled repRNA products obtained in the in vitro reconstitution assay is shown. The membrane fraction of the whole cell extracts prepared from eEF1A mutant strains were mixed with the supernatant fraction of CFE prepared from WT eEF1A (lanes 6-10) or the supernatant fraction of CFE from the mutant strains were added to the membrane fraction from the wt strain (lanes [11] [12] [13] [14] [15] . The reconstituted extracts were programmed with purified recombinant TBSV p33/p92 pol and (+)repRNA. Bottom panel: Western blot analysis shows the amount of endogenous eEF1A in various fractions (see above) prepared from yeast expressing various mutants of eEF1A. doi:10.1371/journal.ppat.1001175.g002

To test if eEF1A stimulates the rate of RNA synthesis in the absence of de novo initiation, we analyzed the amount of 39terminal extension (39-TEX) RNA products, which are generated from an internal primer by the TCV RdRp (Fig. 3D ) [55] . Addition of purified eEF1A did not increase the amount of 39TEX products (lanes 2, 4, 6, Fig. 3D ), suggesting that the elongation step The TBSV (+)RNA template was the short 39 end region (SL1/SL2/SL3), which contain the promoter region (SL1) for initiation and the replication silencer element (within SL3) that down-regulates initiation. The second template was SL1m with a point mutation within the promoter sequence, which is being used more efficiently by the TCV RdRp in vitro. Note that eEF1A has been shown to bind to the replication silencer element. The RdRp assay had two steps: first, the shown components were incubated at room temperature to facilitate their interaction, followed 5 min latter the addition of the shown component and the ribonucleotides to start RNA synthesis. The RdRp activity in samples containing the template RNA and the RdRp were chosen as 100% (lanes 3-4). (B) Detection of abortive RdRp products in the in vitro assay. 15% PAGE/UREA gel was used to resolve the 4-10 nt long products produced during initiation followed by rapid termination. Note that abortive RNA products are characteristic products for RNA polymerases that initiate de novo (in the absence of a traditional primer). (C) Lack of stimulation of 39-terminal extension by eEF1A in vitro. The template RNAs (shown schematically) contain a common artificial hairpin structure at the 39 end that facilitates 39-TEX by the TCV RdRp. The black bar represents 3 different sequences in the three constructs, derived from RIV(+)(includes SL1/SL2/SL3 sequences), RIII(2) and RIII(+) of DI-72 RNA, respectively. The gel image shows the results of 39-TEX in the presence of 0 or 1 mg eEF1A as shown in a TCV RdRp assay. doi:10.1371/journal.ppat.1001175.g003 during complementary RNA synthesis is not affected by eEF1A. Altogether, the obtained in vitro TCV RdRp data suggest that eEF1A can mostly stimulate the initiation step during de novo viral (2)RNA synthesis.

To further test the function of eEF1A in TBSV replication, we used chemical inhibitors of eEF1A, including Didemnin B (DB) and Gamendazole (GM). DB inhibits the activity of GTP-bound eEF1A during translation by binding to a pocket in eEF1A involved in the interaction with the aminoacylated tRNA and the nucleotide exchange factor eEF1Balpha [59, 60] . GM has been shown to inhibit the actin bundling function, while it does not inhibit protein translation or GTP binding functions of eEF1A [61] . We found that both DB and GM efficiently inhibited TBSV repRNA replication in the in vitro assay with CFE, which contains the endogenous eEF1A (Fig. 4A ). Time-course experiments revealed that the inhibition by DB was the most effective when the inhibitor was added at the beginning or during the first 10-15 min of the assay (Fig. 4B , lanes 2-5), while GM inhibited the cell-free replication of TBSV repRNA when added not only at the beginning, but up to 40 min after the start of the assay (lanes [12] [13] [14] [15] [16] [17] . It is known that the recruitment of the viral RNA and replication proteins as well as the assembly of the viral replicase complex take place during the first 40-60 min in the cell-free assay [31] . Since DB could inhibit translation, we also tested the effect of another translation inhibitor, namely cycloheximide, which did not affect TBSV repRNA replication in our assay (Fig. S3) . These data suggest that the inhibition by DB and GM is unlikely through decreased translation in the replication assay. Therefore, the above data are consistent with the model that DB and GM interfere with the assembly of the viral replicase complex in the CFE. Also, GM seems to be a more potent inhibitor of TBSV replication than DB.

To further test if DB and GM can interfere with the assembly of the tombusvirus replicase complex, we performed a two-step in vitro assembly/replication assay, also based on CFE containing endogenous eEF1A [31] . In this assay, first, we only provide ATP and GTP in addition to the replication proteins, the (+)repRNA and CFE, which can support the assembly of the replicase, but cannot perform RNA synthesis due to the lack of CTP and UTP [31] . After 1 hr incubation, once the replicase assembly had taken place, we collected the membrane fraction of the CFE by centrifugation and removed the supernatant containing the unbound p33, p92, repRNA as well as the cytosolic fraction of the CFE. Then we added all four rNTPs (including 32 P-labeled UTP) to the membrane fraction of the CFE to allow for RNA synthesis by the pre-assembled replicase complex (second step, Fig. 4C ) [31] . Interestingly, adding either DB or GM during the first step resulted in robust inhibition of TBSV repRNA synthesis during the second step of the assay (Fig. 4C, lanes 2-3 versus 1) , whereas providing the same amount of DB and GM at the beginning of the second step did not result in inhibition of repRNA replication (lanes 4-6) . These data support a model that DB and GM could inhibit the assembly of the tombusvirus replicase complex, but not the RNA synthesis by the already assembled replicase. Similarly, DB and GM failed to inhibit TBSV RNA synthesis in an in vitro assay with a highly purified RdRp from yeast (Fig. S4A) .

Since the assembly of the tombusvirus replicase also depends on events prior to the replicase assembly step, such as template RNA binding by the viral replication proteins/host proteins (such as eEF1A), and template recruitment to intracellular membranes [21, 52] , we also tested the effect of DB and GM on these processes as well based on purified recombinant eEF1A. We found that GM strongly interfered with the binding of eEF1A to the viral RNA in an EMSA assay (Fig. 4D, lanes 3-6 versus 2) , whereas DB did not affect the binding under the assay conditions (lanes 9-12). Since DB binds only weakly to eEF1A in solution, but it binds much more effectively to eEF1A in the presence of GTP and the ribosome [62] , we also performed in vitro co-purification experiments. First, 35 S-labeled eEF1A was produced in an in vitro translation system (containing ribosome and GTP) and, second, biotin-labeled viral (+)repRNA was added. After short incubation in the absence or presence of various amount of DB, we performed affinity-purification of the viral RNA. Phosphoimaging revealed that eEF1A was co-purified with the viral RNA and the amount of protein co-purified with the viral (+)repRNA was inhibited by increasing amount of DB in the assay (Fig. 4E , compare lane 1 with [2] [3] [4] [5] . This demonstrated that DB inhibits the binding of eEF1A to the viral repRNA. Moreover, both DB and GM interfered with the recruitment of the viral template RNA to the membrane of the CFE containing endogenous eEF1A (Fig. 4F,  lanes 5-8 versus 3-4) . On the other hand, DB and GM do not seem to affect the interaction between eEF1A and p33 or p92 replication proteins in vitro (Fig. S4B) . Altogether, these data suggest that inhibition of eEF1A function by DB and GM could block several steps during the assembly of the tombusvirus replicase complex, including template binding by eEF1A and viral RNA recruitment into replication.

(+)RNA virus replicases contain viral-and host-coded components, which likely provide many yet undefined functions to facilitate robust virus replication in infected cells. Translation factors, such as eEF1A, are among the most common host factors recruited for (+)RNA virus replication. eEF1A is an integral component of several viral replicases, including the highly purified tombusvirus replicase complex. Since eEF1A is an essential G protein involved in translation elongation, it is difficult to obtain evidence for its direct involvement in virus replication in living cells. Indeed, down-regulation of eEF1A in cells has led not only to decreased TBSV repRNA accumulation, but also reduced p33 levels [25] . However, using a small set of functional eEF1A mutants defective in various functions revealed that eEF1A is involved in stabilization of p33 replication protein in yeast [25] .

Based on the previous successful strategy of analyzing eEF1A mutants, here we generated ,6,000 random mutants covering the entire eEF1A sequence and found four mutants, which greatly affected TBSV repRNA accumulation in yeast (Fig. 1A) . Among these mutants, C42, C53 and C62 increased TBSV repRNA replication. Importantly, this effect by the eEF1A mutants was not due to changing the translation efficiency of p33/p92 pol , but likely via directly altering viral replication and affecting the activity of the viral replicase. On the other hand, N21 mutant of eEF1A resulted in decreased TBSV RNA accumulation and also led to reduction in the level of p33 replication protein. This is reminiscent of the previously characterized GDP-binding mutant T 22 S [25], which supported greatly reduced level of viral RNA replication and p33 accumulation due to shortened half-life of p33. Overall, N21 mutant further supports that one of the functions of eEF1A in TBSV replication is to stabilize the p33 replication protein in yeast.

In addition to this genetic evidence on the relevance of eEF1A in TBSV replication in yeast, we also obtained additional supporting data by showing that chemical inhibitors of eEF1A, such as DB and GM, strongly inhibited replication of TBSV eEF1A Role in the Assembly of Virus Replicase repRNA in the cell-free replication assay (Fig. 4A ). Since we used the same amount of purified recombinant p33/p92 pol in this in vitro assay (i.e., translation in the CFE is not needed for production of p33/p92 pol ), the role of eEF1A in TBSV replication must be separate from its role in protein translation. Altogether, these data strongly established that eEF1A is directly involved in TBSV replication, independent of the role of eEF1A in protein translation. Fig. 2 . DB (150 mM) and GM (100 mM) were added at various time points and the replicase assay was stopped after 3 hours for each treatment, followed by RNA analysis in a denaturing PAGE gel. The replicase activity in the samples containing DMSO added at the 0 time point was chosen as 100%. (C) A step-wise approach was used to separate the possible effect of DB and GM during either the assembly of the TBSV replicase or RNA synthesis steps. In step 1, the purified recombinant TBSV p33, p92 pol and (+)repRNA were added to the whole cell extract in the presence of ATP and GTP, which only supports the assembly of the TBSV replicase, but prevents RNA synthesis. This was followed by removal of the extra amount of p33, p92 pol and repRNA, which were not bound to the membranes of cell-free extract, and then by the standard replicase assay in a buffer containing 32 P-UTP and ATP, CTP and GTP (step ''RNA synthesis''). The denaturing PAGE analysis of the 32 P-labeled repRNA products obtained is shown. Note that DB (150 mM) and GM (100 mM) were added to the assay either at the beginning (prior to replicase assembly) or after the replicase assembly. See further details in panel B. (D) The effect of DB and GM on binding between the purified eEF1A and 32 P-labeled template RNA (SL1/SL2/SL3) based on EMSA. The bound and unbound RNAs are pointed at by arrowheads. GM and DB were applied in the following amounts: 0, 5, 50, 250, and 1000 mM. Note that the amount of unbound RNA in the absence of eEF1A (lane 1) was chosen as 100%. (E) The inhibitory effect of DB on co-purification of eEF1A with the viral repRNA. WT 35 S-labeled eEF1A was produced in a translation assay using rabbit reticulocyte lysate, followed by incubation with biotin-labeled DI-72(+) repRNA in the presence of 0, 50, 150, 500 and 1000 mM DB. Then the repRNA was captured with streptavidin-coated magnetic beads, followed by elution of the co-purified proteins from the beads. SDS-PAGE analysis shows the amount of co-purified 35 S-labeled eEF1A. (F) The inhibitory effect of DB and GM on the template recruitment step in vitro. Purified recombinant p33/p92 and 32 P-labeled DI-72 (+)repRNA (indicated as W, lanes 1 and 3-8) or C 99 -G mutant (+)repRNA (indicated as M, lane 2) were added to a whole cell extract (CFE) in the presence of DMSO (control), 100 mM GM or 150 mM DB, followed by centrifugation/washing to remove the 32 P-labeled repRNA that is not bound to the membrane. Then the membrane-bound RNA was analyzed in a denaturing PAGE gel. Note that the recruitment deficient C 99 -G mutant repRNA bound to the membrane nonspecifically (,20% level). doi:10.1371/journal.ppat.1001175.g004 eEF1A selectively enhances minus-strand synthesis during TBSV replication

The identified eEF1A mutants were also useful to dissect the functions of eEF1A in TBSV replication. Based on a cell-free TBSV replication assay in CFE prepared from yeast expressing the C42, C53 or C62 mutants, we found that the minus-strand synthesis was enhanced by ,3-fold, while the rate of plus-strand synthesis was proportionate with (2)RNA synthesis, resulting in ,10-fold more (+) than (2)RNA products for wt and each mutant.

We confirmed a direct role for eEF1A in RNA synthesis in vitro by using a highly purified eEF1A and the recombinant TCV RdRp, which is closely homologous with the TBSV p92 pol . Interestingly, it seems that eEF1A stimulates the RdRp activity directly, since pre-incubation of eEF1A and the RdRp prior to the RdRp assay led to the highest level of stimulation of (2)RNA synthesis (Fig. 3A) . On the other hand, pre-incubation of eEF1A with the TBSV-derived template RNA led only to ,2-fold increase in RNA synthesis in vitro (Fig. 3A) . Analyzing the amount of short abortive RdRp products, which are produced through initiation followed quickly by abortive termination [57] , in the in vitro assays revealed that eEF1A strongly enhanced the initiation of minus-strand synthesis (Fig. 3B) . Although the actual mechanism of stimulation of RdRp activity by eEF1A is currently unknown, we propose that eEF1A might facilitate the proper and efficient binding of the RdRp to the 39 terminal sequence of the viral RNA prior to initiation of (2)-strand synthesis (Fig. 5) . Accordingly, eEF1A was shown to bind to the so-called replication silencer sequence (RSE) in the 39-UTR, which is required for the assembly of the viral replicase complex [22, 58] . The binding of eEF1A-RdRp complex to the RSE might assist in placing the RdRp over the 39-terminal promoter sequence, thus facilitating the initiation of (2)RNA synthesis starting from the 39-terminal cytosine. Similar function of eEF1A in stimulation of (2)RNA synthesis has been proposed for WNV, based on mutations in the viral RNA within the eEF1A binding sequence that reduced the binding affinity of RNA to eEF1A and inhibited (2)RNA synthesis in infected cells [46] . eEF1A stimulates the assembly of the viral replicase complex during TBSV replication Recent intensive work revealed that the assembly of the viral replicase complex is a regulated process involving viral-and host factors, cellular membranes and the viral (+)RNA [8, 10, 19, 63, 64, 65] . The assembly of the viral replicase also depends on steps occurring prior to the actual assembly process, such as selection of the viral template RNA and the recruitment of (+)RNA/protein factors to the sites of assembly. Although our current understanding is rather poor about the factors involved and their functions during replicase assembly, rapid progress is being made in this area due to the development of a new cell-free assay based on yeast CFE [30, 31] . The yeast CFE is capable of assembling the tombusvirus replicase complex in vitro in 40-60 min in the presence of recombinant p33/p92 pol and the viral (+)repRNA [31] , allowing for studies on direct roles of various factors. We find that inhibition of eEF1A activity by either DB or GM also inhibited the assembly of the tombusviral replicase complex based on time-course experiments (Fig. 4B ) as well as a direct replicase assembly assay (Fig. 4C) . On the contrary, the replicase activity was not inhibited by these compounds after the assembly took place (Fig. 4B-C) . It is possible that after the formation of the eEF1A-RdRp-repRNA complex DB or GM are not effective in inhibiting the stimulatory effect of eEF1A on the RNA synthesis by the viral RdRp. Additional in vitro experiments with purified tombusvirus replicase preparations confirmed the lack of inhibition of RNA synthesis by DB or GM (Fig. S4A ) on pre-assembled virus replicases.

The inhibition of the tombusvirus replicase complex by DB or GM might come from the ability of these compounds to inhibit the template RNA recruitment step (Fig. 4F ). If the recruitment of the viral (+)RNA is inhibited, then the assembly of the viral replicase cannot take place in yeast or in vitro [21, 22, 31] . A target for GM and DB could be the inhibition of binding between eEF1A and the viral (+)RNA (Fig. 4D, E) . Since the actual steps during the replicase assembly process are not yet known, it is possible that eEF1A might play additional roles in the assembly of the viral replicase complex.

The presented data are also in agreement with the function of eEF1A as a chaperone of the viral RdRp. Binding between the eEF1A and RdRp might alter the structure of the RdRp that favors de novo initiation for RNA synthesis. Indeed, the chaperone activity of eEF1A and its bacterial homolog EF-Tu has been shown before [66, 67] . Moreover, the EF-Tu-EF-Ts complex is thought to function in the Qbeta replicase complex as a chaperone for maintaining the active conformation of the RdRp protein [68] .

Overall, the current work demonstrates two major functions for eEF1A in TBSV replication (Fig. 5) : (i) stimulation of the assembly of the viral replicase complex, likely by facilitating the recruitment of the viral RNA template into the replicase; and (ii) enhancement of the minus-strand synthesis by promoting the initiation step. These roles for eEF1A are separate from its canonical role in host and viral protein translation.

Saccharomyces cerevisiae strain BY4741 (MATa his3D1 leu2D0 met15D0 ura3D0) was obtained from Open Biosystems (Huntsville, AL, USA). Plasmid-borne TEF1/2 TKY strains (MATa ura3-52 leu2-3, 112 trp1-D1 lys2-20 met2-1 his4-713 tef1::LEU2 tef2D pTEF2 URA3) were published before [69, 70, 71, 72] . The plasmid pESCHIS4-ADH-His33/CUP1-DI-72 expressing Cucumber necrosis virus (CNV) p33 and the TBSV replicon RNA, called DI-72, was described earlier [25] . The LYS2-based plasmid pRS317-Tet-His92, expressing CNV p92 under the control of Tetracycline-regulatable (Tet) promoter was constructed as follows: the Tet promoter sequence was obtained from pCM189-His92/Tet [73] by digestion with EcoRI and BamHI, and CNV p92 coding sequences from pGAD-His92 [7] digested with BamHI and PstI, followed by ligation into pRS317 vector treated with EcoRI and PstI. To generate mutations within TEF1 coding sequence by random mutagenesis, we constructed the TRP1-based plasmid pRS314-pTEF1-TEF1, which expressed TEF1 under the control of its native promoter. The TEF1 promoter sequence, the TEF1 coding region and the Cyc1 terminator sequences were amplified by PCR with the following primer pairs, #2764 (CCGCGAGCTCATAGCTTCAAAAT-GTTTCTAC)/#2765 (CCGCGGATCCGTAATTAAAACT-TAGATTAGATTGC), #2768 (CCGCGGATCCAAAATGGG-TAAAGAGAAGTCTC)/#1877 (CCGCCTCGAGTTATTTC-TTAGCAGCCTTTTGAGCAGC), and #2769 CCGCCTCG-AGGAGGGCCGCATCATGTAA/#2770 (CCGCGGTACCA-GCTTGCAAATTAAAGCCTTC), respectively. This was followed by cloning the PCR products into pRS314 digested with SacI and KpnI.

The mutagenic PCR conditions were as follows: 50 mM KCl, 10 mM Tris (pH 8.3 at 25uC), 7 mM MgCl 2 , 0.3 mM MnCl 2 , 1 mM dCTP and dTTP each, 0.2 mM dGTP and dATP each, 0.2 mM of each primer, 20 pM of template DNA and 10 units of Taq polymerase in a 10 ml reaction volume in 10 aliquots. The PCR was performed for 30 cycles at 94uC for 1 min, 50uC for 1 min, and 72uC for 1 min in a conventional thermal cycler. Three overlapping ,300-500 bp N-, central-and C-terminal segments of the TEF1 gene were amplified separately by PCR using primer pairs: #2767 (GTT-TCAGTTTCATTTTTCTTGTTC)/#2788 (GAGTCCATCT-TGTTGACAG), #2787 (CATCAAGAACATGATTACTGGT-AC)/#2790 (GACGTTACCTCTTCTGATTTC) and #2789 (CGGTGTCATCAAGCCAGGT/#2771, (TTCGGTTAGAGC-GGATGTGG), respectively.

Yeast strain TKY102 was co-transformed with constructs pESCHIS4-ADH-His33/CUP1-DI-72 and pRS317-Tet-His92 to induce TBSV repRNA replication according to standard Lithium acetate-PEG protocol [74] . The transformed yeast cultures were grown in a Synthetic Complete (SC) media with 2% glucose lacking leucine, histidine, lysine and uracil (SC-ULHK 2 ) by shaking at 29uC overnight. To completely suppress TBSV replication before induction, 1 mg/ml Doxycycline was added to the media to inhibit the expression of p92. The plasmid pool carrying the randomly mutated TEF1 gene was introduced into the yeast cells already transformed with the two virus expression plasmids by in vivo gap repair mechanism via homologous recombination (Fig. S1A) [75] . Briefly, pRS314-pTEF1-TEF1 was digested with enzymes to truncate the TEF1 coding sequence, and then the digested plasmid was recovered. The gapped plasmid (5-10 mg) was transformed together with overlapping PCR (20 mg) products carrying the TEF1 mutations created by random mutagenic PCR (see above). The transformed yeast cells were selected on SC media lacking uracil, tryptophan, leucine, histidine and lysine. The colonies were further streaked onto SC media plate lacking tryptophan, leucine, histidine and lysine (SC-TLHK 2 ) with 0.1% (w/v final) 5-Fluoroorotic Acid (5-FOA) media to select against the URA3-based wild-type TEF1 plasmid (Fig. S1A) . This selection was repeated once and the loss of URA3 plasmid was confirmed by the inability of the yeast strains to grow on uracil-minus media. The yeast cells carrying the randomly mutated TEF1 were grown at 29uC for 24 h in SC-TLHK 2 media with 50 mM CuSO 4 to induce virus replication. Total RNA extraction from yeast cells and Northern blotting and Western blotting were done as previously described [7, 25] .

Whole cell yeast extract capable of supporting TBSV replication in vitro was prepared as described [31] . The in vitro TBSV replication assays were performed in 20-ml total volume containing 2 ml of whole cell extract, 0.5 mg DI-72 (+)repRNA transcript, 400 ng purified MBP-p33, 100 ng purified MBP-p92 pol (both recombinant proteins were purified from E. coli), 30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 5 mM magnesium acetate, 0.13 M sorbitol, 0.4 ml actinomycin D (5 mg/ml), 2 ml of 150 mM creatine phosphate, 0.2 ml of 10 mg/ml creatine kinase, 0.2 ml of RNase inhibitor, 0.2 ml of 1 M dithiothreitol (DTT), 2 ml of 10 mM ATP, CTP, and GTP and 0.25 mM UTP and 0.1 ml of [ 32 P]UTP [31] . The reaction mixture was incubated at 25uC for 3 h. The reaction was terminated by adding 100 ml stop buffer (1% sodium dodecyl sulfate [SDS] and 0.05 M EDTA, pH 8.0), followed by phenol-chloroform extraction, isopropanol-ammonium acetate precipitation, and a washing step with 70% ethanol as described [52] . The newly synthesized 32 P-labeled RNA products were separated by electrophoresis in a 5% polyacrylamide gel (PAGE) containing 0.56Tris-borate-EDTA (TBE) buffer with 8 M urea. To detect the double-stranded RNA (dsRNA) in the cell-free replication assay, the 32 P-labeled RNA samples were divided into two aliquotes: one half was loaded onto the gel without heat treatment in the presence of 25% formamide, while the other half was heat denatured at 85uC for 5 min in the presence of 50% formamide [31] . S1 nuclease digestion to remove single-stranded 32 P-labeled RNA was performed at 37uC for 30 min in a buffer containing 5 mM sodium acetate (pH 4.5 at 25uC), 0.28 M NaCl, 4.5mM ZnSO4 and 40 U S1 nuclease (Boehringer).

Fractionation of the whole cell extract was done according to [52] . The total extract was centrifuged at 21,0006 g at 4uC for 10 min to separate the ''soluble'' (supernatant) and ''membrane'' (pellet) fraction. The pellet was re-suspended and washed with buffer A (30 mM HEPES-KOH pH 7.4, 150 mM potassium acetate, and 5 mM magnesium acetate) followed by centrifugation at 21,0006 g at 4uC for 10 min and re-suspension of the pellet in buffer A. In vitro TBSV replication in the fractions was performed as described [31] .

Expression and purification of the recombinant TBSV p33 and p92 and TCV p88C replication proteins from E. coli were carried out as described earlier with modifications [54] . Briefly, the expression plasmids were transformed separately into E. coli strain BL21 Rosetta (DE3). Protein expression was induced using isopropyl b-D-thiogalactopyranoside (IPTG) for 8 h at 16uC, then the cells were collected by centrifugation (5,000 rpm for 5 min). The recombinant TCV p88C protein was purified on an amylose resin column (NEB), as described [54] . The cells were suspended and sonicated in MBP column buffer containing 20 mM Tris-Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, 10 mM b-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride (PMSF). The sonicated extract was then centrifuged at 27,000 g for 10 min, followed by incubation with amylose resin (NEB) for 1 h at 4uC. After washing the resin 3 times with the column buffer and once with a low salt column buffer (25 mM NaCl), the proteins were eluted with a low salt column buffer containing 0.18% (V/W) maltose and 6% (V/V) glycerol and stored at 280uC. MBP-p33 and MBP-p92 pol were purified as above, except 30 mM HEPES-KOH pH 7.4 was used instead of 20 mM Tris-Cl pH 8.0. eEF1A was purified from yeast as described [76] and stored in aliquots at the vapor temperature of liquid nitrogen. Protein fractions used for the replication assays were 95% pure, as determined by SDS-PAGE.

Yeast strains (WT, C42, C53, C62) were transformed with plasmids pESCHIS4-ADH-HF33/CUP1-DI-72 expressing 6XHis-and Flag-tagged CNV p33 and the TBSV DI-72 repRNA, and pRS317-Tet-His92, expressing CNV p92 under the control of Tet promoter [25] . Co-purification was done according to a previously described procedure with the following modification [25] . Gel mobility shift assay (EMSA) and co-purification of eEF1A-repRNA EMSA was performed in a 10 ml-reaction containing 20 mM HEPES [pH 7.6], 50 mM KCl, 2 mM MgCl 2 , 1 mM DTT, 0.1 mM EDTA, 10% [vol/vol] glycerol, 10 U of RNase inhibitor, 10 nM 32 P-labeled DI-72 (+) RNA probe and 0.5 mg purified eEF1A protein [76] . Reactions were incubated at room temperature for 20 min and then resolved by 4% nondenaturing polyacrylamide gel as described previously [25] .

For in vitro eEF1A-repRNA co-purification, DI-72(+) repRNA was biotin-labeled in standard T7 transcription reaction in the presence of 20 mM Biotin-16-UTP (Roche). After the T7 transcription, the unincorporated biotin-UTP was removed on a Bio-Rad mini gel filtration column. The biotinylated RNA was immobilized on a column containing Streptavidin MagneSphere Paramagnetic Particles (SA-PMPs). Briefly, a 30-ml suspension of SA-PMPs (Promega) was washed three times with 1 ml of water and re-suspended in 16 Phosphate Buffered Saline (PBS). Biotinylated DI-72(+) RNA (5 mg) was then added to the suspension of SA-PMPs, followed by 30 min incubation at 4uC with gentle rotation. The SA-PMPs were collected on the side of the tube in a magnetic stand and washed 3 times with 16 PBS buffer. eEF1A was translated in vitro and labeled with 35 In vitro TCV p88C RdRp assay

The TCV RdRp reactions were carried out as previously described for 2 h at 25uC [54] . Briefly, the RdRp reactions were performed in a 20 ml reaction containing 50 mM Tris-HCl (pH 8.2), 10 mM MgCl 2 , 10 mM DTT, 1.0 mM each ATP, CTP, and GTP, 0.01 mM UTP plus 0.1 ml of [ 32 P]UTP, 7 pmol template RNA, 2 pmol affinity-purified MBP-p88C. 20 pmol eEF1A was added to the reaction at the beginning or as indicated in the text and Fig. 3 legend. The 32 P-labeled RNA products were analyzed by electrophoresis in a 5% or 15% PAGE/8 M urea gel [57] . The 86-nt 39 noncoding region of TBSV genomic RNA was used as the template in the RdRp assay [25, 54] .

The use of eEF1A inhibitors in the in vitro replicase assembly assay Purified Didemnin B (NSC 325319) was kindly provided by the Natural Products Branch, NCI (Bethesda, MD, USA), while Gamendazole was a generous gift from Dr. Tash (University of Kansas Medical Center). Both chemicals were dissolved in DMSO (the final concentration was 20 mM). The concentrations of chemical and time point of the addition of the chemicals to the in vitro reaction are indicated in the text. The cell-free TBSV replicase assay and the in vitro TBSV replicase assembly assay were performed according to [31] . Briefly, the purified recombinant TBSV p33, p92 pol and (+) repRNA were added to the cell-free reaction in the presence of 1.0 mM ATP and GTP in step 1. After incubation at 25uC for 1 h, the in vitro reactions were centrifuged 21,0006 g at 4uC for 10 min. The supernatant containing extra p33, p92 pol and repRNA, which were not bound to the membranes in the cell-free extract, was discarded, while the membrane pellet was re-suspended in a standard in vitro replicase assay buffer containing [ 32 P]-UTP and ATP, CTP, and GTP, and incubated at 25uC for 3 h [31] .

The TBSV viral RNA gets recruited to the membrane from the soluble fraction with the help of TBSV replication proteins and host factors present in the yeast CFE. The in vitro RNA recruitment reaction was performed according to [31] , except that 32 P-labeled DI-72 (+)repRNA were used and rCTP, rUTP, 32 P-labeled UTP, and Actinomycin D were omitted from the reaction. As a negative control, a recruitment-deficient repRNA, termed C 99 -G mutant, was used (Fig. 4F , lane 2) [23] . This mutant RNA is not recognized by p33/p92 replication proteins and it does not replicate in plants, in yeast or in the CFE in vitro [23, 31, 52, 77] . The RNA recruitment assay results in the assembly of the functional viral replicase, when wt repRNA is used, and nonfunctional replicase when the C 99 -G mutant is used in the assay (J. Pogany and P.D. Nagy, not shown) [31] . Inhibitors DB and GM were added at final concentration of 150 and 100 mM, respectively. After two hours of incubation at room temperature, 1 ml of reaction buffer was added to the in vitro assay, followed by incubation on ice for 10 min. Samples were centrifuged at 35,0006 g for 1h, and the pellet was washed with 1 ml reaction buffer, followed by centrifugation at 35,0006 g for 10 min. The membrane-bound repRNA was extracted from the pellet by adding 0.1 ml stop buffer and 0.1 ml phenol/chloroform and vortexing, followed by isopropanol/ammonium acetate precipitation [52] . The RNA samples were analyzed by denaturing PAGE and phophoimaging as described [52] . Figure S1 Schematic presentation of the random mutagenesis strategy used to obtain 6,000 eEF1A mutants. (A) The yeast strain (tef1Dtef2D carried a plasmid that expressed one of the random eEF1A (TEF1) mutants from the native promoter. Each yeast strain also carried pESCHIS4-ADH-His33/CUP1-DI-72 and pRS317-Tet-His92 to induce TBSV repRNA replication as described in the M&M section. (B) Additional experiments on the effect of eEF1A mutations on TBSV repRNA accumulation in yeast. The yeast strain expressed only one form of eEF1A, as indicated. Top panel: Replication of the TBSV repRNA was measured by Northern blotting 24 h after initiation of TBSV replication. (C) The accumulation level of repRNA was normalized based on the rRNA (the 18S ribosomal RNA levels were estimated by Northern blotting). Panels (D), (E) and (F) show the accumulation of p92 pol , p33 and eEF1A, respectively, estimated by Western blotting using anti-His and anti-eEF1A antibody. (G) SDS-PAGE analysis of total protein extract from the above yeast strains, after Coomassie blue-staining. Found at: doi:10.1371/journal.ppat.1001175.s001 (0.15 MB PDF) Figure S2 Binding of eEF1A to TBSV and TCV replication proteins in vitro. (A) MBP-tagged TCV p88C (lacking the p28overlapping domain from the N-terminus), MBP-TBSV p92, MBP-TBSV p92C (lacking the p33-overlapping domain from the N-terminus) and MBP-TBSV p33 or MBP (1 mg each) were separately immobilized on amylose beads, followed by incubation with a cytosolic extract prepared from yeast. The bound host proteins were eluted from the beads and were analyzed by 10% SDS-PAGE and detected via Western blotting using anti-eEF1A antibody (Top panel). The affinity-purified recombinant MBP-TCV p88C, MBP-TBSV p92, MBP-TBSV p92C, MBP-TBSV p33 and MBP were analyzed by 10% SDS-PAGE and Coomassie blue-staining (Bottom panel). (B) The effect of eEF1A mutations on binding to the viral p33 and p92 proteins in vitro. MBP-tagged p92, p33 or MBP were separately immobilized on amylose beads, followed by incubation with a cytosolic extract prepared from yeast expressing wt or mutated eEF1A. The bound eEF1A was eluted from the beads and were analyzed by 10% SDS-PAGE and detected via Western blotting using anti-eEF1A antibody (Top panel). The affinity-purified recombinant MBP-TBSV p92, MBP-TBSV p33 and MBP were analyzed by 10% SDS-PAGE and Coomassie blue-staining (Bottom panel). (C) The effect of eEF1A mutations on binding to the viral repRNA. CFE containing WT or mutated eEF1A was incubated with biotin-labeled DI-72(+) repRNA. Then the repRNA was captured with streptavidincoated magnetic beads, followed by elution of the co-purified proteins from the beads. Western blot analysis shows the amount of co-purified eEF1A using anti-eEF1A antibody. Found at: doi:10.1371/journal.ppat.1001175.s002 (0.12 MB PDF) Figure S3 Lack of inhibition of TBSV repRNA replication by Cyclohexamide in a cell-free TBSV replicase assay. The cell-free TBSV replicase assay was performed as described in Fig. 4 . Cyclohexamide was added in the following amounts: 0, 2, 10, 50, 100 mg/ml. The membrane-bound tombusvirus replicase in a yeast lysate was solubilized with Triton X-100/SB3-10 detergent, followed by purification on a FLAG-affinity column as described. The activity of the affinity-purified TBSV replicase was tested on the same amount of DI-72(2) RNA added to each sample. DB (panel on the left) and GM (panel on the right) were added in the following amounts: 0, 100, 150, 200, 250 mM for DB and 0, 25, 50, 100, 200 mM for GM. Denaturing PAGE analysis of the 32 P-labeled RNA products obtained with the purified tombusvirus replicase is shown. Note that this replicase preparation is only capable of complementary RNA synthesis on the added template RNA, but incapable of supporting a full cycle of replication. (B) The effect of GM and DB on binding to the viral p33 and p92 proteins in vitro. MBP-tagged p92 and p33 were separately immobilized on amylose beads, followed by incubation in the presence of 0 or 100 mM GM or 150 mM DB with a cytosolic extract prepared from yeast expressing wt eEF1A. The bound eEF1A was eluted from the beads and were analyzed by 10% SDS-PAGE and detected via Western blotting using anti-eEF1A antibody (Top panel). The affinity-purified recombinant MBP-TBSV p92, MBP-TBSV p33 and MBP were analyzed by 10% SDS-PAGE and Coomassie blue-staining (Bottom panel). Found at: doi:10.1371/journal.ppat.1001175.s004 (0.09 MB PDF)

RNA interference screen for human genes associated with West Nile virus infection

Genomewide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition

Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus

Screening of the yeast yTHC collection identifies essential host factors affecting tombusvirus RNA recombination

Identification of essential host factors affecting tombusvirus RNA replication based on the yeast Tet promoters Hughes Collection

Genome-wide screen identifies host genes affecting viral RNA recombination

Yeast genome-wide screen reveals dissimilar sets of host genes affecting replication of RNA viruses

Host factors in positive-strand RNA virus genome replication

Brome Mosaic Virus RNA Replication: Revealing the Role of the Host in RNA Virus Replication

Yeast as a model host to explore plant virus-host interactions

Viral RNA replication. With a little help from the host

Comparison of the replication of positive-stranded RNA viruses of plants and animals

Replication of tobacco mosaic virus RNA

Tomato bushy stunt virus Co-Opts the RNA-Binding Function of a Host Metabolic Enzyme for Viral Genomic RNA Synthesis

Yeast as a model host to dissect functions of viral and host factors in tombusvirus replication

Host factors involved in West Nile virus replication

Viral and cellular proteins involved in coronavirus replication

Advances in the molecular biology of tombusviruses: gene expression, genome replication, and recombination

Multiple roles of viral replication proteins in plant RNA virus replication

Yeast as a model host to study replication and recombination of defective interfering RNA of Tomato bushy stunt virus

Purification of the cucumber necrosis virus replicase from yeast cells: role of coexpressed viral RNA in stimulation of replicase activity

Role of an internal and two 39-terminal RNA elements in assembly of tombusvirus replicase

Specific binding of tombusvirus replication protein p33 to an internal replication element in the viral RNA is essential for replication

Recombination between defective tombusvirus RNAs generates functional hybrid genomes

Translation elongation factor 1A is a component of the tombusvirus replicase complex and affects the stability of the p33 replication co-factor

Ubiquitin-Conjugating Enzyme Is a Component of the Tombusvirus Replicase Complex and Ubiquitinates p33 Replication Protein

Proteomics analysis of the tombusvirus replicase: Hsp70 molecular chaperone is associated with the replicase and enhances viral RNA replication

Suppression of viral RNA recombination by a host exoribonuclease

A temperature sensitive mutant of heat shock protein 70 reveals an essential role during the early steps of tombusvirus replication

A key role for heat shock protein 70 in the localization and insertion of tombusvirus replication proteins to intracellular membranes

In vitro assembly of the Tomato bushy stunt virus replicase requires the host Heat shock protein 70

Proteasome-mediated degradation of cotranslationally damaged proteins involves translation elongation factor 1A

Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology

Function and structure in phage Qbeta RNA replicase. Association of EF-Tu-Ts with the other enzyme subunits

Membranebound tomato mosaic virus replication proteins participate in RNA synthesis and are associated with host proteins in a pattern distinct from those that are not membrane bound

Translation elongation factor-1 alpha interacts with the 39 stem-loop region of West Nile virus genomic RNA

Functions of the 39-Untranslated Regions of Positive Strand Rna Viral Genomes

Eukaryotic elongation factor 1A interacts with Turnip mosaic virus RNAdependent RNA polymerase and VPg-Pro in virus-induced vesicles

Eukaryotic elongation factor 1A interacts with the upstream pseudoknot domain in the 39 untranslated region of tobacco mosaic virus RNA

Translation elongation factor-1alpha, La, and PTB interact with the 39 untranslated region of dengue 4 virus RNA

The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1

Hepatitis C virus NS4A inhibits cap-dependent and the viral IRES-mediated translation through interacting with eukaryotic elongation factor 1A

In vivo interaction between Tobacco mosaic virus RNA-dependent RNA polymerase and host translation elongation factor 1A

Translation elongation factor 1-alpha interacts specifically with the human immunodeficiency virus type 1 Gag polyprotein

Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA

Interaction between the Cellular Protein eEF1A and the 39-Terminal Stem-Loop of West Nile Virus Genomic RNA Facilitates Viral Minus-Strand RNA Synthesis

eEF1A binding to aminoacylated viral RNA represses minus strand synthesis by TYMV RNA-dependent RNA polymerase

Quantitative assessment of EF-1alpha.GTP binding to aminoacyl-tRNAs, aminoacyl-viral RNA, and tRNA shows close correspondence to the RNA binding properties of EF-Tu

Proteome survey reveals modularity of the yeast cell machinery

Genome-wide screens for identification of host factors in viral replication

Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex

Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast

Heterologous RNA replication enhancer stimulates in vitro RNA synthesis and template-switching by the carmovirus, but not by the tombusvirus, RNA-dependent RNA polymerase: implication for modular evolution of RNA viruses

Comparison of turnip crinkle virus RNA-dependent RNA polymerase preparations expressed in Escherichia coli or derived from infected plants

Mechanism of RNA recombination in carmo-and tombusviruses: evidence for template switching by the RNA-dependent RNA polymerase in vitro

Partial purification and characterization of Cucumber necrosis virus and Tomato bushy stunt virus RNA-dependent RNA polymerases: similarities and differences in template usage between tombusvirus and carmovirus RNA-dependent RNA polymerases

A novel 39-end repair mechanism in an RNA virus

A replication silencer element in a plus-strand RNA virus

Natural products as probes of cell biology: 20 years of didemnin research

Structural basis for the binding of didemnins to human elongation factor eEF1A and rationale for the potent antitumor activity of these marine natural products

Gamendazole, an orally active indazole carboxylic acid male contraceptive agent, targets HSP90AB1 (HSP90BETA) and EEF1A1 (eEF1A), and stimulates Il1a transcription in rat Sertoli cells

Inhibition of protein synthesis by didemnin B: how EF-1alpha mediates inhibition of translocation

Seeking membranes: positive-strand RNA virus replication complexes

Wrapping things up about virus RNA replication

Virus factories: associations of cell organelles for viral replication and morphogenesis

Interaction of the eukaryotic elongation factor 1A with newly synthesized polypeptides

Chaperone properties of bacterial elongation factor EF-G and initiation factor IF2

Qbetaphage resistance by deletion of the coiled-coil motif in elongation factor Ts

Mutations in a GTP-binding motif of eukaryotic elongation factor 1A reduce both translational fidelity and the requirement for nucleotide exchange

Translational misreading: mutations in translation elongation factor 1alpha differentially affect programmed ribosomal frameshifting and drug sensitivity

Improper organization of the actin cytoskeleton affects protein synthesis at initiation

Unique classes of mutations in the Saccharomyces cerevisiae G-protein translation elongation factor 1A suppress the requirement for guanine nucleotide exchange

Host transcription factor Rpb11p affects tombusvirus replication and recombination via regulating the accumulation of viral replication proteins

Transformation of yeast by lithium acetate/singlestranded carrier DNA/polyethylene glycol method

A rapid method for localized mutagenesis of yeast genes

Guanine nucleotide exchange factor independence of the G-protein eEF1A through novel mutant forms and biochemical properties

The p92 polymerase coding region contains an internal RNA element required at an early step in Tombusvirus genome replication

The authors thank Drs. Daniel Barajas 

Conceived and designed the experiments: ZL JP PDN. Performed the experiments: ZL JP ST. Analyzed the data: ZL JP TGK PDN. Contributed reagents/materials/analysis tools: ZL AME TGK. Wrote the paper: ZL PDN.