key: cord-0000125-odnpx3ib authors: Klein, Guy; Kim, Jaekyung; Himmeldirk, Klaus; Cao, Yanyan; Chen, Xiaozhuo title: Antidiabetes and Anti-obesity Activity of Lagerstroemia speciosa date: 2007-03-14 journal: Evid Based Complement Alternat Med DOI: 10.1093/ecam/nem013 sha: 20c05a8e1616b61a78300ee5911bc70bd1c952d3 doc_id: 125 cord_uid: odnpx3ib The leaves of Lagerstroemia speciosa (Lythraceae), a Southeast Asian tree more commonly known as banaba, have been traditionally consumed in various forms by Philippinos for treatment of diabetes and kidney related diseases. In the 1990s, the popularity of this herbal medicine began to attract the attention of scientists worldwide. Since then, researchers have conducted numerous in vitro and in vivo studies that consistently confirmed the antidiabetic activity of banaba. Scientists have identified different components of banaba to be responsible for its activity. Using tumor cells as a cell model, corosolic acid was isolated from the methanol extract of banaba and shown to be an active compound. More recently, a different cell model and the focus on the water soluble fraction of the extract led to the discovery of other compounds. The ellagitannin Lagerstroemin was identified as an effective component of the banaba extract responsible for the activity. In a different approach, using 3T3-L1 adipocytes as a cell model and a glucose uptake assay as the functional screening method, Chen et al. showed that the banaba water extract exhibited an insulin-like glucose transport inducing activity. Coupling HPLC fractionation with a glucose uptake assay, gallotannins were identified in the banaba extract as components responsible for the activity, not corosolic acid. Penta-O-galloyl-glucopyranose (PGG) was identified as the most potent gallotannin. A comparison of published data with results obtained for PGG indicates that PGG has a significantly higher glucose transport stimulatory activity than Lagerstroemin. Chen et al. have also shown that PGG exhibits anti-adipogenic properties in addition to stimulating the glucose uptake in adipocytes. The combination of glucose uptake and anti-adipogenesis activity is not found in the current insulin mimetic drugs and may indicate a great therapeutic potential of PGG. Type 2 diabetes has developed into a worldwide epidemic (1) . Ironically, the dramatic increase in the prevalence of type 2 diabetes can be attributed to the rapid economic development and correlated to changes in lifestyle within the last 50 years. Type 2 diabetes is closely associated with obesity. Up to 90% of the patients in the US with type 2 diabetes are either overweight or obese (2, 3) . It seems likely that the readily available high calorie food and a sedentary life style are major causes for obesity. Obesity contributes to insulin resistance and type 2 diabetes. Reducing obesity and stopping weight gain constitutes a way to slow down the rate of occurrence of type 2 diabetes. Type 2 diabetes is caused by insulin resistance, which is defined as defective insulin signaling and a decreased insulin efficiency to induce glucose transport from the blood into key target cells such as muscle and fat (adipocyte) cells (3) . In general, obesity leads to hyperglycemia, which in turn leads to and exacerbates insulin resistance. Insulin resistance, if not treated, results in hyperinsulinemia and eventually leads to full blown type 2 diabetes (3, 4) . Obesity or excessive adiposity, particularly visceral adiposity, contributes to and worsens insulin resistance (2, 5) . Most antidiabetic drugs are hypoglycemic or anti-hyperglycemic (blood glucose level reducing). However, most of these drugs are, to different extents, weight gain promoting (adipogenic) (6, 7) . Thus, these drugs treat one of the key symptoms of type 2 diabetes, hyperglycemia, but exacerbate the condition of being overweight or obese, one of the leading causes of type 2 diabetes. Therefore, while these drugs are beneficial over the short term, they are not optimal for long term health of type 2 diabetic patient. The most desirable situation would be the development of new types of antidiabetic drugs that are either hypoglycemic or anti-hyperglycemic without the side effect of promoting weight gain (adiposity). Herbal medicines known to be useful in diabetes treatment may be able to lead to compounds with such a combination of ideal therapeutic properties (8) (9) (10) (11) (12) (13) . Lagerstroemia speciosa (Fig. 1) , also called banaba in the Tagalog language of the Philippines, is a tropical plant found in many parts of Southeast Asia including the Philippines, Vietnam, Malaysia and southern China. It is a tree that can grow as tall as 20 m. Despite growing in several countries, only in the Philippines are the dried and shredded banaba leaves known to be used as a treatment for diabetes and kidney disease. It is not clear if banaba plants grown in different countries are equally effective in the treatment of diabetes. Garcia published the first research on banaba's insulinlike, hypoglycemic effect as early as 1940 (14) (15) (16) (17) (18) . Later, the popular use of banaba in the Philippines was noticed and led to its introduction in Japan. However, it was not until 50 years after Garcia's first publication that scientific interest in banaba's potential for the treatment of diabetes resurfaced. Scientists from countries including Japan, the Philippines, Korea and the United States are currently studying banaba. Lagerstroemia speciosa (banaba) has become relatively popular in the form of health-promoting tea products in Eastern Asia and the United States. In 1996, Kakuda et al. (19) studied banaba's antidiabetic activity by preparing water and methanol extracts of the plant. After feeding the extracts to hereditary type 2 diabetic KK-Ay/Ta Jcl mice, they found that food containing either 5% of water extract (BE) or 3% of methanol extract was effective in reducing blood glucose and insulin levels (P50.05) (19) . It is interesting to note that the total cholesterol in treated mice was also significantly reduced, but the plasma triglyceride level remained unchanged (19) . In a second study by Kakuda's research group, food containing 5% banaba water extract was used to feed female obese KK-Ay/Ta Jcl mice. Obese mice treated with banaba extract had a significantly reduced body weight (10%) compared with control mice fed with a regular diet (20) . No change in food intake was observed (20) . Interestingly, it was also discovered that liver triglyceride content was reduced by more than 40% in the banaba extract-treated mice. In addition, the parametrial adipose tissue was 10% lighter (P50.01) (20) . However, as in the previous antidiabetic activity study of BE in animals (19) , both the identity of the effective component(s) and the mechanism for the activity were not studied. Nevertheless, these two studies clearly demonstrated the in vivo antidiabetic and anti-weight gaining efficacy of the extract. In 1993, a group of scientists from Hiroshima University used an Ehrlich ascites tumor cell line coupled with a bioassay guided fractionation to screen compounds isolated by HPLC from banaba extract in order to identify the effective antidiabetic component (21) . Corosolic acid (2a-hydroxyursoloic acid) was identified as the effective compound in the methanol extract of banaba leaves in a glucose uptake assay ( Fig. 2A, 21) . However, this result should be considered with caution since the tumor cell line used in this study is a very unusual and unconventional cell line for diabetes studies or antidiabetic compound screening. Furthermore, the result could not explain the discrepancy that both the banaba water extract and the methanol extract were active in antidiabetic and anti-obesity animal studies since corosolic acid only exists in the methanol extract (19) (20) (21) . Realizing the potential problems associated with the cell line selection and assay method, the researchers acknowledged in a later publication that corosolic acid 'could not represent the whole activity of the banaba extract' (22) . Consequently, the researchers switched their cell model from the original tumor cells to a natural cell target of insulin, adipocytes, in order to allow for the 'isolation and the identification of more active compounds using the improved methodology' (22) . After HPLC purification, ellagitannins were identified in the water extract of banaba as the activators of glucose transport in fat cells with a glucose uptake assay (22) . One of the most potent ellagitannins was named 'Lagerstroemin' (Fig. 2, 22) . In a recent study, the same group reported the activation of the insulin receptor (IR) by Lagerstroemin (Fig. 2, 23) . In this study, Lagerstroemin was able to induce phosphorylation of the b-subunit of IR at 150 mM (23) but the mechanism responsible for IR activation was not found. The researchers speculated that Lagerstroemin could act intracellularly or bind to the insulin receptor (IR) extracellularly. In 2004, a separate group of researchers from several universities found that glucose transporter 4 (GLUT4) translocation from the intracellular microsomal membrane to the plasma membrane was significantly increased in the muscle cells of mice treated orally with corosolic acid (P50.05, 24). This result is both interesting and puzzling. GLUT4 is the major glucose transporter protein in both muscle and adipocytes and GLUT4 is insulin-responsive (4, 25) . However, since corosolic acid does not possess insulin-like glucose transport stimulatory activity, the process that leads to GLUT4 translocation is not known. The GLUT4 membrane translocation mechanism initiated by corosolic acid as described (24) must be independent of the IR mediated signaling pathway since corosolic acid does not use this pathway for its activity. In 2006, the same group of researchers found that corosolic acid significantly reduces blood glucose levels and plasma insulin levels in KK-Ay diabetic mice (26) . Additionally, this group of researchers published another article in 2006 in which they showed that corosolic acid significantly lowered blood glucose levels at the 90 min mark in an oral glucose tolerance test done on type 2 diabetic patients (27) . However, no statistical difference between the treated group and the control group was found for any other time point during the test (27) . Our interest in the isolation and identification of antidiabetic compounds from natural sources initiated our investigation of banaba extracts with 3T3-L1 adipocytes as a cell model and a radioactive glucose uptake assay as a screening method for the identification of potential antidiabetic compounds. In our study, we confirmed that the water and methanol extracts of banaba leaves exhibit glucose transport stimulatory activity (28) . In addition, we showed that the activity induced by the extract had a concentration-activity profile similar to that of insulin, suggesting that the activity of banaba extract (BE) might be triggered via an insulin-like mechanism (28) . Furthermore, we demonstrated that the same BE also inhibits adipocyte differentiation (28) . 3T3-L1 preadipocytes treated with methylisobutylxanthine, dexamethasone, and insulin (MDI) and BE did not differentiate into adipocytes as they normally do under the sole influence of MDI. This result indicated that BE inhibits the adipocyte differentiation activity induced by MDI. Unlike the glucose transport stimulation, the anti-differentiation activity is anti-insulin like, since insulin plays an important role in adipocyte differentiation and is a component of MDI ('I' in MDI stands for Insulin). Thus, BE differs from insulin in that it is anti-adipogenic whereas insulin is adipogenic, which is considered to be a negative side effect of insulin. In order to identify the component(s) in BE responsible for the antidiabetic activity, our first goal was to confirm that corosolic acid was responsible for the glucose transport stimulatory activity. Since tannins comprise up to 40% of the material in the extract, we were interested in separating it from corosolic acid first. To our surprise, after removing tannins by either gelatin or bovine serum albumin tannin precipitations (29), the remaining extract was not able to induce glucose transport. We concluded that the glucose transport activity was caused by the tannin component of the extract, and not corosolic acid (29) . Furthermore, we tested pure corosolic acid and found that it was not able to stimulate glucose transport in our cell model (Fig. 3) . Although we cannot exclude the possibility that corosolic acid may have some antidiabetic activity, we can eliminate corosolic acid from having the insulin-like glucose transport stimulatory activity found in adipocytes. It should be noted that banaba extracts prepared from banaba leaves from different sources may have different chemical compositions, which in turn may lead to different experimental results (our lab used dried banaba leaves from the Philippines). After testing corosolic acid, we also tested the corosolic acid-based banaba extract product glucosol (30) . The glucose uptake assay revealed that glucosol was activating glucose transport. Its activity was dose-dependent (Fig. 4) . However, since corosolic acid itself does not have any insulin receptor mediated glucose transport activity, the effect of glucosol is likely to originate from other chemical compounds in the banaba extract and not from corosolic acid. Tannins comprise a large and diverse class of polyphenolic compounds (31, 32) . Our search for the active components in the tannin fraction of BE was made much easier after we discovered that commercially available tannic acid (TA) shows similar glucose transport stimulatory activity to BE (29) . The main components of TA are gallotannins, a subclass of the tannins usually consisting of a glucose core connected to a variable number of galloyl groups via ester bonds (Fig. 5) . TA is known to exhibit various health-beneficial activities (33) (34) (35) (36) . As a constituent of red wine, it has been shown to effectively reduce blood glucose levels in type 2 diabetes patients (37) and the production of endothelin-1 (38), a key protein factor intimately involved in the development of cardiovascular disease (38) . In our study, TA was found to be much more potent and efficacious than the ellagitannin Lagerstroemin (29) . Therefore, TA was chosen as the focus of our research for isolating active compounds from the tannin fraction Figure 3 . Absence of glucose transport stimulatory activity of corosolic acid in adipocytes. Pure corosolic acid in aqueous solution was added to 3T3-L1 adipocytes grown in wells of 6-well cell culture plates to induce glucose transport by a commonly used procedure (28, 29) . Cells treated with either 1 mM insulin or 30 mM penta-galloyl-glucose (PGG) were used as positive controls. Cells treated with water vehicle served as negative controls. Samples were in triplicate in the experiment, and the experiment was repeated three times. No difference was found by a one-way ANOVA statistical analysis between the negative (vehicle) control and corosolic acid samples at any concentration. of BE. Components of TA were separated by HPLC, and active fractions were identified with a glucose uptake assay in 3T3-L1 adipocytes (29) . The study led to the discovery of penta-O-galloyl-D-glucopyranose (PGG) as the most effective compound in TA (Fig. 5, 39 ). Both anomers of PGG occur in nature (40) . The a-anomer was found to be slightly more active than the b-form in its glucose transport stimulatory activity (39) . Corosolic Acid and Lagerstroemin versus Tannic Acid and PGG: Which are More Bioactive? In the ellagitannin study mentioned earlier, Lagerstroemin exhibits glucose transport stimulation at 40 mM with an EC 50 of 80 mM (22) . In comparison, aand b-PGG exhibit activity at a concentration as low as 10 mM with EC 50 of 17 and 18 mM (39) . In other words, aand b-PGG are about five times more potent than Lagerstroemin in stimulating glucose transport. We would like to emphasize that both aand b-PGG possess the adipogenesis inhibitory activity exhibited by the banaba extract (28) and by TA (29) . This suggests that these two activities are associated with the same molecules. It also indicates the functional differences between insulin (glucose transport inducing and adipogenic) and PGG (glucose transport inducing but anti-adipogenic). Both compounds may have the potential to reduce hyperglycemia without increasing adiposity, a very desirable combination of properties that insulin lacks. The Lagerstroemin study also showed that its glucose transport inducing activity is about 54% of that of insulin (22) . In comparison, aand b-PGG showed 60-70% of insulin's glucose transport inducing activity (39) . . Glucose transport stimulatory activity of glucosol as compared with banana extract. Banaba extract (BE) was prepared in house (28) . Glucosol (30) was purchased. Glucosol was compared with BE in a regular glucose uptake assay (28, 29) . Samples were assayed in triplicates, and the assay was repeated three times. The result of the assays was analyzed with a one-way ANOVA. *P50.05, **P50.01, ***P50.001. All samples were compared with the negative (vehicle) control. Thus, aand b-PGG are at least 30% more effective than Lagerstroemin (Table 1 ). It is interesting that PGG possesses many other health-beneficial bioactivities, such as anticancer (41, 42) , anti-inflammation (43, 44) , antivirus (anti-HIV, 45; anti-SARS, 46) and antioxidant activity (47, 48) . From the known studies, we conclude that tannin molecules are responsible for the insulin-like glucose transport stimulatory activity of the banaba extract. Gallotannins such as PGG appear to be more potent and efficacious than ellagitanins such as Lagerstroemin in IR binding, IR activation and glucose transport induction. Corosolic acid does not possess any insulin-like glucose transport stimulatory activity. If its antidiabetic activity can be confirmed, it is likely to be induced via a noninsulin like, indirect mechanism. Although tannins were identified as the effective component for the insulin like glucose transport inducing activity in banaba extract, the most effective tannin molecule in the extract has not been identified. A well designed bioassay (glucose uptake assay) guided isolation should be able to complete this task. In the past 10 years, other studies regarding banaba extracts or chemicals derived from banaba extracts were reported (49) (50) (51) (52) . These studies indicate that banaba extracts contain interesting biomedical substances that have attracted significant scientific attention. More detailed studies at molecular and cellular levels as well as in animal models are required to elucidate banaba extract's antidiabetic activity and other health beneficial activities such as its anti-adipogenesis activity. Corosolic acid -0 Insulin 1 nM 156 *Activity of b-PGG was arbitrarily assigned as 100. Global and societal implications of the diabetes epidemic Redefining type 2 diabetes: 'diabesity' or 'obesity dependent diabetes mellitus'? 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