This study explored the initial validity and reliability of the Multicultural Supervision Inventory-MSI, previously named the Multicultural Supervision Competence Scale-MSCS (Pope-Davis, Toporek, Ortega-Villalobos, 1999). The Multicultural Supervision Inventory consists of two parallel versions intended to be used in both research and counselor training activities. A supervisor version assesses the multicultural competence of supervisors from their own perspective, and a supervisee version assesses the multicultural competence of supervisors from the perspective of the supervisee. Exploratory factor analyses yielded distinct underlying factor structures for the supervisor and supervisee versions. MSI scores yielded adequate reliability coefficients (supervisor MSI γ = .88, supervisee MSI γ = .92) for both samples. This study provided a foundation for further validation work.