id sid tid token lemma pos z316pz5339d 1 1 arboviruses arbovirus NOUN z316pz5339d 1 2 , , PUNCT z316pz5339d 1 3 chikungunya chikungunya NOUN z316pz5339d 1 4 ( ( PUNCT z316pz5339d 1 5 chikv chikv PROPN z316pz5339d 1 6 ) ) PUNCT z316pz5339d 1 7 and and CCONJ z316pz5339d 1 8 dengue dengue PROPN z316pz5339d 1 9 are be AUX z316pz5339d 1 10 transmitted transmit VERB z316pz5339d 1 11 by by ADP z316pz5339d 1 12 the the DET z316pz5339d 1 13 same same ADJ z316pz5339d 1 14 major major ADJ z316pz5339d 1 15 vectors vector NOUN z316pz5339d 1 16 i.e. i.e. X z316pz5339d 1 17 aedes aedes PROPN z316pz5339d 1 18 aegypti aegypti PROPN z316pz5339d 1 19 and and CCONJ z316pz5339d 1 20 aedes aedes PROPN z316pz5339d 1 21 albopictus albopictus NOUN z316pz5339d 1 22 . . PUNCT z316pz5339d 2 1 in in ADP z316pz5339d 2 2 this this DET z316pz5339d 2 3 study study NOUN z316pz5339d 2 4 we we PRON z316pz5339d 2 5 first first ADV z316pz5339d 2 6 tested test VERB z316pz5339d 2 7 a a DET z316pz5339d 2 8 set set NOUN z316pz5339d 2 9 of of ADP z316pz5339d 2 10 seven seven NUM z316pz5339d 2 11 antiviral antiviral ADJ z316pz5339d 2 12 hammerhead hammerhead NOUN z316pz5339d 2 13 ribozymes ribozyme NOUN z316pz5339d 2 14 ( ( PUNCT z316pz5339d 2 15 hrzs hrzs NOUN z316pz5339d 2 16 ) ) PUNCT z316pz5339d 2 17 targeting target VERB z316pz5339d 2 18 chikv chikv ADJ z316pz5339d 2 19 structural structural ADJ z316pz5339d 2 20 proteins protein NOUN z316pz5339d 2 21 as as ADP z316pz5339d 2 22 potential potential ADJ z316pz5339d 2 23 antivirals antiviral NOUN z316pz5339d 2 24 . . PUNCT z316pz5339d 3 1 suppression suppression NOUN z316pz5339d 3 2 of of ADP z316pz5339d 3 3 chikv chikv NOUN z316pz5339d 3 4 infectivity infectivity NOUN z316pz5339d 3 5 was be AUX z316pz5339d 3 6 confirmed confirm VERB z316pz5339d 3 7 by by ADP z316pz5339d 3 8 challenging challenge VERB z316pz5339d 3 9 hygromycin hygromycin PROPN z316pz5339d 3 10 selected select VERB z316pz5339d 3 11 vero vero PROPN z316pz5339d 3 12 cells cell NOUN z316pz5339d 3 13 transfected transfecte VERB z316pz5339d 3 14 with with ADP z316pz5339d 3 15 plasmids plasmids NOUN z316pz5339d 3 16 expressing express VERB z316pz5339d 3 17 each each PRON z316pz5339d 3 18 of of ADP z316pz5339d 3 19 the the DET z316pz5339d 3 20 ribozymes ribozyme NOUN z316pz5339d 3 21 , , PUNCT z316pz5339d 3 22 demonstrating demonstrate VERB z316pz5339d 3 23 ribozyme ribozyme NOUN z316pz5339d 3 24 # # SYM z316pz5339d 3 25 9 9 NUM z316pz5339d 3 26 and and CCONJ z316pz5339d 3 27 # # SYM z316pz5339d 3 28 14 14 NUM z316pz5339d 3 29 were be AUX z316pz5339d 3 30 most most ADV z316pz5339d 3 31 effective effective ADJ z316pz5339d 3 32 in in ADP z316pz5339d 3 33 inhibiting inhibit VERB z316pz5339d 3 34 replication replication NOUN z316pz5339d 3 35 of of ADP z316pz5339d 3 36 chikv chikv NOUN z316pz5339d 3 37 by by ADP z316pz5339d 3 38 2 2 NUM z316pz5339d 3 39 and and CCONJ z316pz5339d 3 40 5 5 NUM z316pz5339d 3 41 orders order NOUN z316pz5339d 3 42 of of ADP z316pz5339d 3 43 magnitude magnitude NOUN z316pz5339d 3 44 respectively respectively ADV z316pz5339d 3 45 at at ADP z316pz5339d 3 46 an an DET z316pz5339d 3 47 moi moi X z316pz5339d 3 48 of of ADP z316pz5339d 3 49 0.0001 0.0001 NUM z316pz5339d 3 50 . . PUNCT z316pz5339d 3 51 to to PART z316pz5339d 3 52 achieve achieve VERB z316pz5339d 3 53 higher high ADJ z316pz5339d 3 54 levels level NOUN z316pz5339d 3 55 of of ADP z316pz5339d 3 56 suppression suppression NOUN z316pz5339d 3 57 clonals clonal NOUN z316pz5339d 3 58 cell cell NOUN z316pz5339d 3 59 populations population NOUN z316pz5339d 3 60 were be AUX z316pz5339d 3 61 generated generate VERB z316pz5339d 3 62 for for ADP z316pz5339d 3 63 each each DET z316pz5339d 3 64 ribozyme ribozyme NOUN z316pz5339d 3 65 enabling enable VERB z316pz5339d 3 66 complete complete ADJ z316pz5339d 3 67 suppression suppression NOUN z316pz5339d 3 68 at at ADP z316pz5339d 3 69 0.0001 0.0001 NUM z316pz5339d 3 70 . . PUNCT z316pz5339d 4 1 effective effective ADJ z316pz5339d 4 2 clones clone NOUN z316pz5339d 4 3 were be AUX z316pz5339d 4 4 selected select VERB z316pz5339d 4 5 based base VERB z316pz5339d 4 6 on on ADP z316pz5339d 4 7 lack lack NOUN z316pz5339d 4 8 of of ADP z316pz5339d 4 9 cytopathic cytopathic ADJ z316pz5339d 4 10 effect effect NOUN z316pz5339d 4 11 ( ( PUNCT z316pz5339d 4 12 cpe cpe PROPN z316pz5339d 4 13 ) ) PUNCT z316pz5339d 4 14 . . PUNCT z316pz5339d 5 1 similar similar ADJ z316pz5339d 5 2 approaches approach NOUN z316pz5339d 5 3 were be AUX z316pz5339d 5 4 attempted attempt VERB z316pz5339d 5 5 using use VERB z316pz5339d 5 6 maxizyme maxizyme NOUN z316pz5339d 5 7 constructed construct VERB z316pz5339d 5 8 from from ADP z316pz5339d 5 9 hrz#9 hrz#9 PROPN z316pz5339d 5 10 and and CCONJ z316pz5339d 5 11 # # SYM z316pz5339d 5 12 14 14 NUM z316pz5339d 5 13 . . PUNCT z316pz5339d 6 1 tcid50 tcid50 ADJ z316pz5339d 6 2 analysis analysis NOUN z316pz5339d 6 3 confirmed confirm VERB z316pz5339d 6 4 complete complete ADJ z316pz5339d 6 5 suppression suppression NOUN z316pz5339d 6 6 of of ADP z316pz5339d 6 7 chikv chikv ADJ z316pz5339d 6 8 replication replication NOUN z316pz5339d 6 9 at at ADP z316pz5339d 6 10 an an DET z316pz5339d 6 11 moi moi NOUN z316pz5339d 6 12 of of ADP z316pz5339d 6 13 0.0001 0.0001 NUM z316pz5339d 6 14 and and CCONJ z316pz5339d 6 15 0.001 0.001 NUM z316pz5339d 6 16 confirming confirm VERB z316pz5339d 6 17 the the DET z316pz5339d 6 18 advantage advantage NOUN z316pz5339d 6 19 of of ADP z316pz5339d 6 20 maxizyme maxizyme NOUN z316pz5339d 6 21 over over ADP z316pz5339d 6 22 hrzs hrzs NOUN z316pz5339d 6 23 . . PUNCT z316pz5339d 7 1 the the DET z316pz5339d 7 2 benefits benefit NOUN z316pz5339d 7 3 of of ADP z316pz5339d 7 4 maxizymes maxizyme NOUN z316pz5339d 7 5 were be AUX z316pz5339d 7 6 further far ADV z316pz5339d 7 7 confirmed confirm VERB z316pz5339d 7 8 by by ADP z316pz5339d 7 9 comparing compare VERB z316pz5339d 7 10 the the DET z316pz5339d 7 11 suppression suppression NOUN z316pz5339d 7 12 potential potential NOUN z316pz5339d 7 13 of of ADP z316pz5339d 7 14 clonals clonal NOUN z316pz5339d 7 15 expressing express VERB z316pz5339d 7 16 dengue dengue NOUN z316pz5339d 7 17 specific specific ADJ z316pz5339d 7 18 maxizymes maxizyme NOUN z316pz5339d 7 19 , , PUNCT z316pz5339d 7 20 connected connect VERB z316pz5339d 7 21 maxizymes maxizyme NOUN z316pz5339d 7 22 , , PUNCT z316pz5339d 7 23 and and CCONJ z316pz5339d 7 24 hrzs hrzs NOUN z316pz5339d 7 25 in in ADP z316pz5339d 7 26 vero vero PROPN z316pz5339d 7 27 cells cell NOUN z316pz5339d 7 28 . . PUNCT z316pz5339d 8 1 tcid50 tcid50 ADJ z316pz5339d 8 2 analysis analysis NOUN z316pz5339d 8 3 demonstrated demonstrate VERB z316pz5339d 8 4 complete complete ADJ z316pz5339d 8 5 suppression suppression NOUN z316pz5339d 8 6 of of ADP z316pz5339d 8 7 dengue dengue NOUN z316pz5339d 8 8 at at ADP z316pz5339d 8 9 an an DET z316pz5339d 8 10 moi moi NOUN z316pz5339d 8 11 of of ADP z316pz5339d 8 12 0.01 0.01 NUM z316pz5339d 8 13 , , PUNCT z316pz5339d 8 14 compared compare VERB z316pz5339d 8 15 to to ADP z316pz5339d 8 16 only only ADV z316pz5339d 8 17 2 2 NUM z316pz5339d 8 18 log log NOUN z316pz5339d 8 19 of of ADP z316pz5339d 8 20 suppression suppression NOUN z316pz5339d 8 21 with with ADP z316pz5339d 8 22 dengue dengue PROPN z316pz5339d 8 23 specific specific PROPN z316pz5339d 8 24 hrzs hrzs PROPN z316pz5339d 8 25 clonals clonal NOUN z316pz5339d 8 26 . . PUNCT z316pz5339d 9 1 all all DET z316pz5339d 9 2 results result NOUN z316pz5339d 9 3 were be AUX z316pz5339d 9 4 further far ADV z316pz5339d 9 5 complemented complement VERB z316pz5339d 9 6 by by ADP z316pz5339d 9 7 caspase-3 caspase-3 PROPN z316pz5339d 9 8 assays assay NOUN z316pz5339d 9 9 and and CCONJ z316pz5339d 9 10 quantitative quantitative ADJ z316pz5339d 9 11 real real ADJ z316pz5339d 9 12 time time NOUN z316pz5339d 9 13 pcr pcr NOUN z316pz5339d 9 14 assays assay NOUN z316pz5339d 9 15 . . PUNCT z316pz5339d 10 1 this this DET z316pz5339d 10 2 study study NOUN z316pz5339d 10 3 also also ADV z316pz5339d 10 4 showed show VERB z316pz5339d 10 5 the the DET z316pz5339d 10 6 usefulness usefulness NOUN z316pz5339d 10 7 of of ADP z316pz5339d 10 8 connected connected ADJ z316pz5339d 10 9 maxizyme maxizyme NOUN z316pz5339d 10 10 over over ADP z316pz5339d 10 11 maxizyme maxizyme NOUN z316pz5339d 10 12 , , PUNCT z316pz5339d 10 13 clearly clearly ADV z316pz5339d 10 14 evident evident ADJ z316pz5339d 10 15 based base VERB z316pz5339d 10 16 on on ADP z316pz5339d 10 17 both both CCONJ z316pz5339d 10 18 tcid50 tcid50 ADJ z316pz5339d 10 19 and and CCONJ z316pz5339d 10 20 quantitative quantitative ADJ z316pz5339d 10 21 real real ADJ z316pz5339d 10 22 time time NOUN z316pz5339d 10 23 pcr pcr NOUN z316pz5339d 10 24 analysis analysis NOUN z316pz5339d 10 25 . . PUNCT z316pz5339d 11 1 hrzs hrzs NOUN z316pz5339d 11 2 # # SYM z316pz5339d 11 3 9 9 NUM z316pz5339d 11 4 and and CCONJ z316pz5339d 11 5 # # SYM z316pz5339d 11 6 14 14 NUM z316pz5339d 11 7 exhibiting exhibit VERB z316pz5339d 11 8 the the DET z316pz5339d 11 9 best good ADJ z316pz5339d 11 10 chikv chikv NOUN z316pz5339d 11 11 suppression suppression NOUN z316pz5339d 11 12 were be AUX z316pz5339d 11 13 used use VERB z316pz5339d 11 14 to to PART z316pz5339d 11 15 generate generate VERB z316pz5339d 11 16 transgenic transgenic NOUN z316pz5339d 11 17 mosquitoe mosquitoe NOUN z316pz5339d 11 18 line line NOUN z316pz5339d 11 19 . . PUNCT z316pz5339d 12 1 both both PRON z316pz5339d 12 2 hrzs hrzs NOUN z316pz5339d 12 3 # # SYM z316pz5339d 12 4 9 9 NUM z316pz5339d 12 5 and and CCONJ z316pz5339d 12 6 # # SYM z316pz5339d 12 7 14 14 NUM z316pz5339d 12 8 promoted promote VERB z316pz5339d 12 9 by by ADP z316pz5339d 12 10 the the DET z316pz5339d 12 11 aedes aedes PROPN z316pz5339d 12 12 aegypti aegypti PROPN z316pz5339d 12 13 t t PROPN z316pz5339d 12 14 - - PUNCT z316pz5339d 12 15 rnaval rnaval PROPN z316pz5339d 12 16 pol pol PROPN z316pz5339d 12 17 iii iii PROPN z316pz5339d 12 18 promoter promoter NOUN z316pz5339d 12 19 were be AUX z316pz5339d 12 20 been be AUX z316pz5339d 12 21 mobilized mobilize VERB z316pz5339d 12 22 into into ADP z316pz5339d 12 23 the the DET z316pz5339d 12 24 mosquito mosquito NOUN z316pz5339d 12 25 genome genome NOUN z316pz5339d 12 26 using use VERB z316pz5339d 12 27 the the DET z316pz5339d 12 28 piggybac piggybac NOUN z316pz5339d 12 29 transposon transposon PROPN z316pz5339d 12 30 vector vector NOUN z316pz5339d 12 31 system system NOUN z316pz5339d 12 32 . . PUNCT z316pz5339d 13 1 splinkerrtte splinkerrtte PROPN z316pz5339d 13 2 pcr pcr PROPN z316pz5339d 13 3 confirmed confirm VERB z316pz5339d 13 4 the the DET z316pz5339d 13 5 establishment establishment NOUN z316pz5339d 13 6 of of ADP z316pz5339d 13 7 nine nine NUM z316pz5339d 13 8 lines line NOUN z316pz5339d 13 9 with with ADP z316pz5339d 13 10 different different ADJ z316pz5339d 13 11 integration integration NOUN z316pz5339d 13 12 sites site NOUN z316pz5339d 13 13 in in ADP z316pz5339d 13 14 aedes aedes PROPN z316pz5339d 13 15 genome genome PROPN z316pz5339d 13 16 , , PUNCT z316pz5339d 13 17 seven seven NUM z316pz5339d 13 18 expressing express VERB z316pz5339d 13 19 hrz hrz NOUN z316pz5339d 13 20 # # SYM z316pz5339d 13 21 9 9 NUM z316pz5339d 13 22 and and CCONJ z316pz5339d 13 23 two two NUM z316pz5339d 13 24 expressing express VERB z316pz5339d 13 25 hrz hrz NOUN z316pz5339d 13 26 # # SYM z316pz5339d 13 27 14 14 NUM z316pz5339d 13 28 . . PUNCT z316pz5339d 14 1 rt rt PROPN z316pz5339d 14 2 - - PUNCT z316pz5339d 14 3 pcr pcr PROPN z316pz5339d 14 4 was be AUX z316pz5339d 14 5 performed perform VERB z316pz5339d 14 6 to to PART z316pz5339d 14 7 confirm confirm VERB z316pz5339d 14 8 expression expression NOUN z316pz5339d 14 9 of of ADP z316pz5339d 14 10 the the DET z316pz5339d 14 11 hrz hrz PROPN z316pz5339d 14 12 transgene transgene NOUN z316pz5339d 14 13 in in ADP z316pz5339d 14 14 each each DET z316pz5339d 14 15 line.the line.the DET z316pz5339d 14 16 transgenic transgenic NOUN z316pz5339d 14 17 mosquitoes mosquito NOUN z316pz5339d 14 18 expressing express VERB z316pz5339d 14 19 either either CCONJ z316pz5339d 14 20 hrz hrz NOUN z316pz5339d 14 21 # # SYM z316pz5339d 14 22 9 9 NUM z316pz5339d 14 23 or or CCONJ z316pz5339d 14 24 # # SYM z316pz5339d 14 25 14 14 NUM z316pz5339d 14 26 , , PUNCT z316pz5339d 14 27 and and CCONJ z316pz5339d 14 28 control control VERB z316pz5339d 14 29 higgs higgs PROPN z316pz5339d 14 30 white white PROPN z316pz5339d 14 31 eye eye PROPN z316pz5339d 14 32 ( ( PUNCT z316pz5339d 14 33 hwe hwe PROPN z316pz5339d 14 34 ) ) PUNCT z316pz5339d 14 35 mosquitoes mosquito NOUN z316pz5339d 14 36 were be AUX z316pz5339d 14 37 blood blood NOUN z316pz5339d 14 38 fed feed VERB z316pz5339d 14 39 over over ADP z316pz5339d 14 40 an an DET z316pz5339d 14 41 artificial artificial ADJ z316pz5339d 14 42 blood blood NOUN z316pz5339d 14 43 meal meal NOUN z316pz5339d 14 44 containing contain VERB z316pz5339d 14 45 the the DET z316pz5339d 14 46 attenuated attenuate VERB z316pz5339d 14 47 strain strain NOUN z316pz5339d 14 48 of of ADP z316pz5339d 14 49 chikv chikv NOUN z316pz5339d 14 50 181/25 181/25 NUM z316pz5339d 14 51 virus virus NOUN z316pz5339d 14 52 . . PUNCT z316pz5339d 15 1 tcid50 tcid50 X z316pz5339d 16 1 analysis analysis NOUN z316pz5339d 16 2 7 7 NUM z316pz5339d 16 3 dpi dpi NOUN z316pz5339d 16 4 on on ADP z316pz5339d 16 5 engorged engorge VERB z316pz5339d 16 6 mosquitoes mosquito NOUN z316pz5339d 16 7 demonstrated demonstrate VERB z316pz5339d 16 8 no no INTJ z316pz5339d 16 9 or or CCONJ z316pz5339d 16 10 minimal minimal ADJ z316pz5339d 16 11 viral viral ADJ z316pz5339d 16 12 replication replication NOUN z316pz5339d 16 13 in in ADP z316pz5339d 16 14 transgenic transgenic NOUN z316pz5339d 16 15 mosquitoes mosquito NOUN z316pz5339d 16 16 compared compare VERB z316pz5339d 16 17 to to ADP z316pz5339d 16 18 control control PROPN z316pz5339d 16 19 hwe hwe PROPN z316pz5339d 16 20 displaying display VERB z316pz5339d 16 21 the the DET z316pz5339d 16 22 viral viral ADJ z316pz5339d 16 23 titer titer NOUN z316pz5339d 16 24 of of ADP z316pz5339d 16 25 2x104 2x104 NUM z316pz5339d 16 26 - - SYM z316pz5339d 16 27 105 105 NUM z316pz5339d 16 28 . . PUNCT z316pz5339d 17 1 all all PRON z316pz5339d 17 2 of of ADP z316pz5339d 17 3 the the DET z316pz5339d 17 4 nine nine NUM z316pz5339d 17 5 transgenic transgenic NOUN z316pz5339d 17 6 lines line NOUN z316pz5339d 17 7 were be AUX z316pz5339d 17 8 confirmed confirm VERB z316pz5339d 17 9 as as ADP z316pz5339d 17 10 at at ADP z316pz5339d 17 11 most most ADV z316pz5339d 17 12 partially partially ADV z316pz5339d 17 13 homozygous homozygous ADJ z316pz5339d 17 14 using use VERB z316pz5339d 17 15 direct direct ADJ z316pz5339d 17 16 pcr pcr NOUN z316pz5339d 17 17 analysis analysis NOUN z316pz5339d 17 18 . . PUNCT z316pz5339d 18 1 engineered engineer VERB z316pz5339d 18 2 transgenic transgenic NOUN z316pz5339d 18 3 mosquitoes mosquito NOUN z316pz5339d 18 4 having have VERB z316pz5339d 18 5 high high ADJ z316pz5339d 18 6 resistance resistance NOUN z316pz5339d 18 7 to to ADP z316pz5339d 18 8 chikv chikv ADJ z316pz5339d 18 9 replication replication NOUN z316pz5339d 18 10 can can AUX z316pz5339d 18 11 be be AUX z316pz5339d 18 12 potentially potentially ADV z316pz5339d 18 13 utilized utilize VERB z316pz5339d 18 14 in in ADP z316pz5339d 18 15 population population NOUN z316pz5339d 18 16 replacement replacement NOUN z316pz5339d 18 17 strategies strategy NOUN z316pz5339d 18 18 to to PART z316pz5339d 18 19 achieve achieve VERB z316pz5339d 18 20 elimination elimination NOUN z316pz5339d 18 21 of of ADP z316pz5339d 18 22 endemic endemic ADJ z316pz5339d 18 23 chikv chikv NOUN z316pz5339d 18 24 . . PUNCT