id sid tid token lemma pos sj139023d62 1 1 the the DET sj139023d62 1 2 past past ADJ sj139023d62 1 3 two two NUM sj139023d62 1 4 decades decade NOUN sj139023d62 1 5 have have AUX sj139023d62 1 6 witnessed witness VERB sj139023d62 1 7 the the DET sj139023d62 1 8 rapid rapid ADJ sj139023d62 1 9 development development NOUN sj139023d62 1 10 of of ADP sj139023d62 1 11 droplet droplet ADJ sj139023d62 1 12 microfluidics microfluidic NOUN sj139023d62 1 13 technologies technology NOUN sj139023d62 1 14 boosted boost VERB sj139023d62 1 15 by by ADP sj139023d62 1 16 the the DET sj139023d62 1 17 invention invention NOUN sj139023d62 1 18 and and CCONJ sj139023d62 1 19 commercialization commercialization NOUN sj139023d62 1 20 of of ADP sj139023d62 1 21 single single ADJ sj139023d62 1 22 molecule molecule NOUN sj139023d62 1 23 analysis analysis NOUN sj139023d62 1 24 , , PUNCT sj139023d62 1 25 single single ADJ sj139023d62 1 26 cell cell NOUN sj139023d62 1 27 analysis analysis NOUN sj139023d62 1 28 , , PUNCT sj139023d62 1 29 and and CCONJ sj139023d62 1 30 material material ADJ sj139023d62 1 31 synthesis synthesis NOUN sj139023d62 1 32 in in ADP sj139023d62 1 33 droplets droplet NOUN sj139023d62 1 34 . . PUNCT sj139023d62 2 1 however however ADV sj139023d62 2 2 , , PUNCT sj139023d62 2 3 those those DET sj139023d62 2 4 recently recently ADV sj139023d62 2 5 commercialized commercialize VERB sj139023d62 2 6 technologies technology NOUN sj139023d62 2 7 are be AUX sj139023d62 2 8 expensive expensive ADJ sj139023d62 2 9 for for ADP sj139023d62 2 10 general general ADJ sj139023d62 2 11 usage usage NOUN sj139023d62 2 12 at at ADP sj139023d62 2 13 the the DET sj139023d62 2 14 point point NOUN sj139023d62 2 15 of of ADP sj139023d62 2 16 need need NOUN sj139023d62 2 17 / / SYM sj139023d62 2 18 use use NOUN sj139023d62 2 19 / / SYM sj139023d62 2 20 care care NOUN sj139023d62 2 21 . . PUNCT sj139023d62 3 1 to to PART sj139023d62 3 2 make make VERB sj139023d62 3 3 advanced advanced ADJ sj139023d62 3 4 droplet droplet ADJ sj139023d62 3 5 microfluidics microfluidic NOUN sj139023d62 3 6 technologies technology NOUN sj139023d62 3 7 more more ADV sj139023d62 3 8 accessible accessible ADJ sj139023d62 3 9 , , PUNCT sj139023d62 3 10 this this DET sj139023d62 3 11 systematic systematic ADJ sj139023d62 3 12 work work NOUN sj139023d62 3 13 is be AUX sj139023d62 3 14 designed design VERB sj139023d62 3 15 and and CCONJ sj139023d62 3 16 developed develop VERB sj139023d62 3 17 . . PUNCT sj139023d62 4 1 in in ADP sj139023d62 4 2 chapter chapter NOUN sj139023d62 4 3 2 2 NUM sj139023d62 4 4 , , PUNCT sj139023d62 4 5 a a DET sj139023d62 4 6 new new ADJ sj139023d62 4 7 method method NOUN sj139023d62 4 8 is be AUX sj139023d62 4 9 invented invent VERB sj139023d62 4 10 and and CCONJ sj139023d62 4 11 validated validate VERB sj139023d62 4 12 for for ADP sj139023d62 4 13 the the DET sj139023d62 4 14 convenient convenient ADJ sj139023d62 4 15 generation generation NOUN sj139023d62 4 16 of of ADP sj139023d62 4 17 uniform uniform ADJ sj139023d62 4 18 microdroplets microdroplet NOUN sj139023d62 4 19 . . PUNCT sj139023d62 5 1 the the DET sj139023d62 5 2 effective effective ADJ sj139023d62 5 3 method method NOUN sj139023d62 5 4 takes take VERB sj139023d62 5 5 advantage advantage NOUN sj139023d62 5 6 of of ADP sj139023d62 5 7 the the DET sj139023d62 5 8 common common ADJ sj139023d62 5 9 pipette pipette NOUN sj139023d62 5 10 liquid liquid NOUN sj139023d62 5 11 handling handling NOUN sj139023d62 5 12 process process NOUN sj139023d62 5 13 and and CCONJ sj139023d62 5 14 simply simply ADV sj139023d62 5 15 by by ADP sj139023d62 5 16 replacing replace VERB sj139023d62 5 17 the the DET sj139023d62 5 18 commercial commercial ADJ sj139023d62 5 19 round round ADJ sj139023d62 5 20 - - PUNCT sj139023d62 5 21 orifice orifice NOUN sj139023d62 5 22 pipette pipette NOUN sj139023d62 5 23 tips tip NOUN sj139023d62 5 24 with with ADP sj139023d62 5 25 elliptical elliptical ADJ sj139023d62 5 26 - - PUNCT sj139023d62 5 27 orifice orifice NOUN sj139023d62 5 28 tips tip NOUN sj139023d62 5 29 , , PUNCT sj139023d62 5 30 uniform uniform NOUN sj139023d62 5 31 droplets droplet VERB sj139023d62 5 32 could could AUX sj139023d62 5 33 be be AUX sj139023d62 5 34 generated generate VERB sj139023d62 5 35 via via ADP sj139023d62 5 36 pipetting pipette VERB sj139023d62 5 37 aqueous aqueous ADJ sj139023d62 5 38 liquid liquid NOUN sj139023d62 5 39 into into ADP sj139023d62 5 40 the the DET sj139023d62 5 41 oil oil NOUN sj139023d62 5 42 phase phase NOUN sj139023d62 5 43 . . PUNCT sj139023d62 6 1 elliptical elliptical ADJ sj139023d62 6 2 - - PUNCT sj139023d62 6 3 orifice orifice NOUN sj139023d62 6 4 tips tip NOUN sj139023d62 6 5 are be AUX sj139023d62 6 6 prepared prepare VERB sj139023d62 6 7 by by ADP sj139023d62 6 8 mechanical mechanical ADJ sj139023d62 6 9 pressing pressing NOUN sj139023d62 6 10 of of ADP sj139023d62 6 11 the the DET sj139023d62 6 12 head head NOUN sj139023d62 6 13 part part NOUN sj139023d62 6 14 of of ADP sj139023d62 6 15 the the DET sj139023d62 6 16 commercial commercial ADJ sj139023d62 6 17 round round ADJ sj139023d62 6 18 - - PUNCT sj139023d62 6 19 orifice orifice NOUN sj139023d62 6 20 tips tip NOUN sj139023d62 6 21 to to PART sj139023d62 6 22 deform deform VERB sj139023d62 6 23 the the DET sj139023d62 6 24 original original ADJ sj139023d62 6 25 round round NOUN sj139023d62 6 26 orifices orifice NOUN sj139023d62 6 27 into into ADP sj139023d62 6 28 elliptical elliptical ADJ sj139023d62 6 29 ones one NOUN sj139023d62 6 30 . . PUNCT sj139023d62 7 1 the the PRON sj139023d62 7 2 higher high ADJ sj139023d62 7 3 the the DET sj139023d62 7 4 deformation deformation NOUN sj139023d62 7 5 degree degree NOUN sj139023d62 7 6 , , PUNCT sj139023d62 7 7 the the DET sj139023d62 7 8 smaller small ADJ sj139023d62 7 9 and and CCONJ sj139023d62 7 10 more more ADV sj139023d62 7 11 uniform uniform ADJ sj139023d62 7 12 the the DET sj139023d62 7 13 generated generate VERB sj139023d62 7 14 droplets droplet NOUN sj139023d62 7 15 . . PUNCT sj139023d62 8 1 the the DET sj139023d62 8 2 reason reason NOUN sj139023d62 8 3 why why SCONJ sj139023d62 8 4 elliptical elliptical ADJ sj139023d62 8 5 orifices orifice NOUN sj139023d62 8 6 help help VERB sj139023d62 8 7 uniform uniform ADJ sj139023d62 8 8 droplet droplet ADJ sj139023d62 8 9 generation generation NOUN sj139023d62 8 10 is be AUX sj139023d62 8 11 that that SCONJ sj139023d62 8 12 the the DET sj139023d62 8 13 rebalance rebalance NOUN sj139023d62 8 14 of of ADP sj139023d62 8 15 young young ADJ sj139023d62 8 16 - - PUNCT sj139023d62 8 17 laplace laplace NOUN sj139023d62 8 18 pressure pressure NOUN sj139023d62 8 19 of of ADP sj139023d62 8 20 the the DET sj139023d62 8 21 pipette pipette NOUN sj139023d62 8 22 - - PUNCT sj139023d62 8 23 tip tip NOUN sj139023d62 8 24 - - PUNCT sj139023d62 8 25 wall wall NOUN sj139023d62 8 26 - - PUNCT sj139023d62 8 27 constrained constrain VERB sj139023d62 8 28 dispersed disperse VERB sj139023d62 8 29 phase phase NOUN sj139023d62 8 30 right right ADV sj139023d62 8 31 out out ADP sj139023d62 8 32 of of ADP sj139023d62 8 33 the the DET sj139023d62 8 34 orifice orifice NOUN sj139023d62 8 35 favors favor VERB sj139023d62 8 36 the the DET sj139023d62 8 37 easy easy ADJ sj139023d62 8 38 and and CCONJ sj139023d62 8 39 quick quick ADJ sj139023d62 8 40 pinch pinch NOUN sj139023d62 8 41 - - PUNCT sj139023d62 8 42 off off NOUN sj139023d62 8 43 of of ADP sj139023d62 8 44 droplets droplet NOUN sj139023d62 8 45 and and CCONJ sj139023d62 8 46 the the DET sj139023d62 8 47 process process NOUN sj139023d62 8 48 is be AUX sj139023d62 8 49 mainly mainly ADV sj139023d62 8 50 governed govern VERB sj139023d62 8 51 by by ADP sj139023d62 8 52 the the DET sj139023d62 8 53 surface surface NOUN sj139023d62 8 54 tension tension NOUN sj139023d62 8 55 between between ADP sj139023d62 8 56 the the DET sj139023d62 8 57 dispersed disperse VERB sj139023d62 8 58 phase phase NOUN sj139023d62 8 59 and and CCONJ sj139023d62 8 60 the the DET sj139023d62 8 61 continuous continuous ADJ sj139023d62 8 62 phase phase NOUN sj139023d62 8 63 , , PUNCT sj139023d62 8 64 which which PRON sj139023d62 8 65 means mean VERB sj139023d62 8 66 the the DET sj139023d62 8 67 process process NOUN sj139023d62 8 68 is be AUX sj139023d62 8 69 insensitive insensitive ADJ sj139023d62 8 70 to to ADP sj139023d62 8 71 moderate moderate ADJ sj139023d62 8 72 flow flow NOUN sj139023d62 8 73 rate rate NOUN sj139023d62 8 74 changes change NOUN sj139023d62 8 75 and and CCONJ sj139023d62 8 76 manual manual ADJ sj139023d62 8 77 pipetting pipetting NOUN sj139023d62 8 78 is be AUX sj139023d62 8 79 possible possible ADJ sj139023d62 8 80 for for ADP sj139023d62 8 81 uniform uniform PROPN sj139023d62 8 82 microdroplet microdroplet PROPN sj139023d62 8 83 generation generation NOUN sj139023d62 8 84 . . PUNCT sj139023d62 9 1 in in ADP sj139023d62 9 2 chapter chapter NOUN sj139023d62 9 3 3 3 NUM sj139023d62 9 4 , , PUNCT sj139023d62 9 5 supporting support VERB sj139023d62 9 6 technologies technology NOUN sj139023d62 9 7 are be AUX sj139023d62 9 8 developed develop VERB sj139023d62 9 9 for for ADP sj139023d62 9 10 stabilizing stabilize VERB sj139023d62 9 11 , , PUNCT sj139023d62 9 12 assembling assemble VERB sj139023d62 9 13 , , PUNCT sj139023d62 9 14 and and CCONJ sj139023d62 9 15 imaging imaging NOUN sj139023d62 9 16 of of ADP sj139023d62 9 17 microdroplets microdroplet NOUN sj139023d62 9 18 so so SCONJ sj139023d62 9 19 that that SCONJ sj139023d62 9 20 the the DET sj139023d62 9 21 microdroplets microdroplet NOUN sj139023d62 9 22 could could AUX sj139023d62 9 23 be be AUX sj139023d62 9 24 more more ADV sj139023d62 9 25 useful useful ADJ sj139023d62 9 26 at at ADP sj139023d62 9 27 the the DET sj139023d62 9 28 point point NOUN sj139023d62 9 29 of of ADP sj139023d62 9 30 need need NOUN sj139023d62 9 31 . . PUNCT sj139023d62 10 1 to to PART sj139023d62 10 2 meet meet VERB sj139023d62 10 3 the the DET sj139023d62 10 4 thermal thermal ADJ sj139023d62 10 5 stability stability NOUN sj139023d62 10 6 requirement requirement NOUN sj139023d62 10 7 of of ADP sj139023d62 10 8 polymerase polymerase NOUN sj139023d62 10 9 chain chain NOUN sj139023d62 10 10 reaction reaction NOUN sj139023d62 10 11 ( ( PUNCT sj139023d62 10 12 pcr pcr NOUN sj139023d62 10 13 ) ) PUNCT sj139023d62 10 14 in in ADP sj139023d62 10 15 droplets droplet NOUN sj139023d62 10 16 , , PUNCT sj139023d62 10 17 hybrid hybrid ADJ sj139023d62 10 18 surfactant surfactant NOUN sj139023d62 10 19 systems system NOUN sj139023d62 10 20 are be AUX sj139023d62 10 21 developed develop VERB sj139023d62 10 22 to to PART sj139023d62 10 23 further far ADV sj139023d62 10 24 stabilize stabilize VERB sj139023d62 10 25 droplets droplet NOUN sj139023d62 10 26 for for ADP sj139023d62 10 27 high high ADJ sj139023d62 10 28 temperatures temperature NOUN sj139023d62 10 29 . . PUNCT sj139023d62 11 1 once once SCONJ sj139023d62 11 2 the the DET sj139023d62 11 3 droplets droplet NOUN sj139023d62 11 4 are be AUX sj139023d62 11 5 sufficiently sufficiently ADV sj139023d62 11 6 stable stable ADJ sj139023d62 11 7 for for ADP sj139023d62 11 8 applications application NOUN sj139023d62 11 9 , , PUNCT sj139023d62 11 10 robust robust ADJ sj139023d62 11 11 handling handling NOUN sj139023d62 11 12 and and CCONJ sj139023d62 11 13 assembly assembly NOUN sj139023d62 11 14 of of ADP sj139023d62 11 15 droplets droplet NOUN sj139023d62 11 16 are be AUX sj139023d62 11 17 highly highly ADV sj139023d62 11 18 welcome welcome ADJ sj139023d62 11 19 . . PUNCT sj139023d62 12 1 herein herein ADV sj139023d62 12 2 , , PUNCT sj139023d62 12 3 readily readily ADV sj139023d62 12 4 available available ADJ sj139023d62 12 5 tape tape NOUN sj139023d62 12 6 - - PUNCT sj139023d62 12 7 based base VERB sj139023d62 12 8 chips chip NOUN sj139023d62 12 9 with with ADP sj139023d62 12 10 shallow shallow ADJ sj139023d62 12 11 centers center NOUN sj139023d62 12 12 are be AUX sj139023d62 12 13 designed design VERB sj139023d62 12 14 and and CCONJ sj139023d62 12 15 easily easily ADV sj139023d62 12 16 fabricated fabricate VERB sj139023d62 12 17 to to PART sj139023d62 12 18 immobilize immobilize VERB sj139023d62 12 19 and and CCONJ sj139023d62 12 20 assemble assemble VERB sj139023d62 12 21 loaded loaded ADJ sj139023d62 12 22 droplets droplet NOUN sj139023d62 12 23 / / SYM sj139023d62 12 24 oil oil NOUN sj139023d62 12 25 into into ADP sj139023d62 12 26 monolayers monolayer NOUN sj139023d62 12 27 for for ADP sj139023d62 12 28 subsequent subsequent ADJ sj139023d62 12 29 handling handling NOUN sj139023d62 12 30 and and CCONJ sj139023d62 12 31 imaging imaging NOUN sj139023d62 12 32 . . PUNCT sj139023d62 13 1 an an DET sj139023d62 13 2 advantage advantage NOUN sj139023d62 13 3 of of ADP sj139023d62 13 4 the the DET sj139023d62 13 5 pipetting pipette VERB sj139023d62 13 6 generated generate VERB sj139023d62 13 7 uniform uniform NOUN sj139023d62 13 8 droplets droplet NOUN sj139023d62 13 9 is be AUX sj139023d62 13 10 that that SCONJ sj139023d62 13 11 the the DET sj139023d62 13 12 diameters diameter NOUN sj139023d62 13 13 of of ADP sj139023d62 13 14 the the DET sj139023d62 13 15 droplets droplet NOUN sj139023d62 13 16 are be AUX sj139023d62 13 17 usually usually ADV sj139023d62 13 18 larger large ADJ sj139023d62 13 19 than than ADP sj139023d62 13 20 150 150 NUM sj139023d62 13 21 um um INTJ sj139023d62 13 22 which which PRON sj139023d62 13 23 allows allow VERB sj139023d62 13 24 imaging imaging NOUN sj139023d62 13 25 of of ADP sj139023d62 13 26 droplets droplet NOUN sj139023d62 13 27 by by ADP sj139023d62 13 28 smartphone smartphone ADJ sj139023d62 13 29 cameras camera NOUN sj139023d62 13 30 . . PUNCT sj139023d62 14 1 for for ADP sj139023d62 14 2 fluorescence fluorescence ADJ sj139023d62 14 3 imaging imaging NOUN sj139023d62 14 4 of of ADP sj139023d62 14 5 the the DET sj139023d62 14 6 droplets droplet NOUN sj139023d62 14 7 , , PUNCT sj139023d62 14 8 a a DET sj139023d62 14 9 common common ADJ sj139023d62 14 10 transilluminator transilluminator NOUN sj139023d62 14 11 with with ADP sj139023d62 14 12 proper proper ADJ sj139023d62 14 13 filtering filtering NOUN sj139023d62 14 14 could could AUX sj139023d62 14 15 be be AUX sj139023d62 14 16 used use VERB sj139023d62 14 17 together together ADV sj139023d62 14 18 with with ADP sj139023d62 14 19 the the DET sj139023d62 14 20 smartphone smartphone NOUN sj139023d62 14 21 to to PART sj139023d62 14 22 reveal reveal VERB sj139023d62 14 23 the the DET sj139023d62 14 24 positive positive ADJ sj139023d62 14 25 droplets droplet NOUN sj139023d62 14 26 with with ADP sj139023d62 14 27 amplified amplify VERB sj139023d62 14 28 fluorescence fluorescence NOUN sj139023d62 14 29 . . PUNCT sj139023d62 15 1 in in ADP sj139023d62 15 2 addition addition NOUN sj139023d62 15 3 , , PUNCT sj139023d62 15 4 a a DET sj139023d62 15 5 thousand thousand NUM sj139023d62 15 6 - - PUNCT sj139023d62 15 7 dollar dollar NOUN sj139023d62 15 8 handheld handheld ADJ sj139023d62 15 9 miniature miniature ADJ sj139023d62 15 10 fluorescence fluorescence PROPN sj139023d62 15 11 microscope microscope PROPN sj139023d62 15 12 also also ADV sj139023d62 15 13 works work VERB sj139023d62 15 14 perfectly perfectly ADV sj139023d62 15 15 for for ADP sj139023d62 15 16 point point NOUN sj139023d62 15 17 - - PUNCT sj139023d62 15 18 of of ADP sj139023d62 15 19 - - PUNCT sj139023d62 15 20 need need NOUN sj139023d62 15 21 droplet droplet ADJ sj139023d62 15 22 imaging imaging NOUN sj139023d62 15 23 . . PUNCT sj139023d62 16 1 in in ADP sj139023d62 16 2 chapter chapter NOUN sj139023d62 16 3 4 4 NUM sj139023d62 16 4 , , PUNCT sj139023d62 16 5 on on ADP sj139023d62 16 6 the the DET sj139023d62 16 7 basis basis NOUN sj139023d62 16 8 of of ADP sj139023d62 16 9 the the DET sj139023d62 16 10 highly highly ADV sj139023d62 16 11 accessible accessible ADJ sj139023d62 16 12 droplet droplet ADJ sj139023d62 16 13 generation generation NOUN sj139023d62 16 14 / / SYM sj139023d62 16 15 stabilization stabilization NOUN sj139023d62 16 16 / / SYM sj139023d62 16 17 assembly assembly NOUN sj139023d62 16 18 / / SYM sj139023d62 16 19 imaging image VERB sj139023d62 16 20 techniques technique NOUN sj139023d62 16 21 , , PUNCT sj139023d62 16 22 representative representative ADJ sj139023d62 16 23 droplet droplet ADJ sj139023d62 16 24 microfluidics microfluidic NOUN sj139023d62 16 25 applications application NOUN sj139023d62 16 26 are be AUX sj139023d62 16 27 conducted conduct VERB sj139023d62 16 28 to to PART sj139023d62 16 29 confirm confirm VERB sj139023d62 16 30 the the DET sj139023d62 16 31 successful successful ADJ sj139023d62 16 32 usage usage NOUN sj139023d62 16 33 of of ADP sj139023d62 16 34 pipette pipette NOUN sj139023d62 16 35 droplets droplet NOUN sj139023d62 16 36 as as ADP sj139023d62 16 37 microreactors microreactor NOUN sj139023d62 16 38 , , PUNCT sj139023d62 16 39 microcompartments microcompartment NOUN sj139023d62 16 40 , , PUNCT sj139023d62 16 41 and and CCONJ sj139023d62 16 42 micro micro NOUN sj139023d62 16 43 - - NOUN sj139023d62 16 44 molds mold NOUN sj139023d62 16 45 / / SYM sj139023d62 16 46 templates template NOUN sj139023d62 16 47 at at ADP sj139023d62 16 48 the the DET sj139023d62 16 49 point point NOUN sj139023d62 16 50 of of ADP sj139023d62 16 51 need need NOUN sj139023d62 16 52 . . PUNCT sj139023d62 17 1 the the DET sj139023d62 17 2 well well ADV sj139023d62 17 3 - - PUNCT sj139023d62 17 4 known know VERB sj139023d62 17 5 droplet droplet ADJ sj139023d62 17 6 digital digital ADJ sj139023d62 17 7 polymerase polymerase NOUN sj139023d62 17 8 chain chain NOUN sj139023d62 17 9 reaction reaction NOUN sj139023d62 17 10 ( ( PUNCT sj139023d62 17 11 ddpcr ddpcr NOUN sj139023d62 17 12 ) ) PUNCT sj139023d62 17 13 is be AUX sj139023d62 17 14 demonstrated demonstrate VERB sj139023d62 17 15 with with ADP sj139023d62 17 16 droplets droplet NOUN sj139023d62 17 17 as as ADP sj139023d62 17 18 microreactors microreactor NOUN sj139023d62 17 19 for for ADP sj139023d62 17 20 accurate accurate ADJ sj139023d62 17 21 sras sra NOUN sj139023d62 17 22 - - PUNCT sj139023d62 17 23 cov-2 cov-2 ADP sj139023d62 17 24 detection detection NOUN sj139023d62 17 25 and and CCONJ sj139023d62 17 26 quantification quantification NOUN sj139023d62 17 27 at at ADP sj139023d62 17 28 low low ADJ sj139023d62 17 29 viral viral ADJ sj139023d62 17 30 concentrations concentration NOUN sj139023d62 17 31 . . PUNCT sj139023d62 18 1 counting counting NOUN sj139023d62 18 2 - - PUNCT sj139023d62 18 3 based base VERB sj139023d62 18 4 quantification quantification NOUN sj139023d62 18 5 with with ADP sj139023d62 18 6 smartphone smartphone NOUN sj139023d62 18 7 images image NOUN sj139023d62 18 8 works work VERB sj139023d62 18 9 well well ADV sj139023d62 18 10 in in ADP sj139023d62 18 11 the the DET sj139023d62 18 12 dynamic dynamic ADJ sj139023d62 18 13 range range NOUN sj139023d62 18 14 of of ADP sj139023d62 18 15 4 4 NUM sj139023d62 18 16 – – SYM sj139023d62 18 17 100 100 NUM sj139023d62 18 18 copies copy NOUN sj139023d62 18 19 ( ( PUNCT sj139023d62 18 20 per per ADP sj139023d62 18 21 20 20 NUM sj139023d62 18 22 µl µl PROPN sj139023d62 18 23 reaction reaction NOUN sj139023d62 18 24 mixture mixture NOUN sj139023d62 18 25 ) ) PUNCT sj139023d62 18 26 and and CCONJ sj139023d62 18 27 the the DET sj139023d62 18 28 calculated calculate VERB sj139023d62 18 29 limit limit NOUN sj139023d62 18 30 - - PUNCT sj139023d62 18 31 of of ADP sj139023d62 18 32 - - PUNCT sj139023d62 18 33 detection detection NOUN sj139023d62 18 34 is be AUX sj139023d62 18 35 ~3.8 ~3.8 PROPN sj139023d62 18 36 copies copy NOUN sj139023d62 18 37 ( ( PUNCT sj139023d62 18 38 per per ADP sj139023d62 18 39 20 20 NUM sj139023d62 18 40 µl µl PROPN sj139023d62 18 41 reaction reaction NOUN sj139023d62 18 42 mixture mixture NOUN sj139023d62 18 43 ) ) PUNCT sj139023d62 18 44 which which PRON sj139023d62 18 45 is be AUX sj139023d62 18 46 comparable comparable ADJ sj139023d62 18 47 to to ADP sj139023d62 18 48 that that PRON sj139023d62 18 49 of of ADP sj139023d62 18 50 commercial commercial ADJ sj139023d62 18 51 ddpcr ddpcr NOUN sj139023d62 18 52 . . PUNCT sj139023d62 19 1 besides besides SCONJ sj139023d62 19 2 ddpcr ddpcr NOUN sj139023d62 19 3 , , PUNCT sj139023d62 19 4 spirulina spirulina NOUN sj139023d62 19 5 microorganisms microorganism NOUN sj139023d62 19 6 / / SYM sj139023d62 19 7 cells cell NOUN sj139023d62 19 8 are be AUX sj139023d62 19 9 encapsulated encapsulate VERB sj139023d62 19 10 and and CCONJ sj139023d62 19 11 cultured cultured ADJ sj139023d62 19 12 in in ADP sj139023d62 19 13 the the DET sj139023d62 19 14 droplets droplet NOUN sj139023d62 19 15 ( ( PUNCT sj139023d62 19 16 proliferation proliferation NOUN sj139023d62 19 17 rate rate NOUN sj139023d62 19 18 : : PUNCT sj139023d62 19 19 96.9 96.9 NUM sj139023d62 19 20 % % NOUN sj139023d62 19 21 ± ± NOUN sj139023d62 19 22 1.8 1.8 NUM sj139023d62 19 23 % % NOUN sj139023d62 19 24 ) ) PUNCT sj139023d62 19 25 to to PART sj139023d62 19 26 show show VERB sj139023d62 19 27 the the DET sj139023d62 19 28 potential potential NOUN sj139023d62 19 29 of of ADP sj139023d62 19 30 using use VERB sj139023d62 19 31 pipetting pipette VERB sj139023d62 19 32 generated generate VERB sj139023d62 19 33 droplets droplet NOUN sj139023d62 19 34 as as ADP sj139023d62 19 35 microcompartments microcompartment NOUN sj139023d62 19 36 for for ADP sj139023d62 19 37 single single ADJ sj139023d62 19 38 microorganism microorganism NOUN sj139023d62 19 39 / / SYM sj139023d62 19 40 cell cell NOUN sj139023d62 19 41 analysis analysis NOUN sj139023d62 19 42 . . PUNCT sj139023d62 20 1 what what PRON sj139023d62 20 2 's be AUX sj139023d62 20 3 more more ADJ sj139023d62 20 4 , , PUNCT sj139023d62 20 5 janus janus ADJ sj139023d62 20 6 microgels microgel NOUN sj139023d62 20 7 are be AUX sj139023d62 20 8 synthesized synthesize VERB sj139023d62 20 9 in in ADP sj139023d62 20 10 droplets droplet NOUN sj139023d62 20 11 to to PART sj139023d62 20 12 prove prove VERB sj139023d62 20 13 the the DET sj139023d62 20 14 utilization utilization NOUN sj139023d62 20 15 of of ADP sj139023d62 20 16 droplets droplet NOUN sj139023d62 20 17 as as ADP sj139023d62 20 18 micro micro NOUN sj139023d62 20 19 - - NOUN sj139023d62 20 20 molds mold NOUN sj139023d62 20 21 / / SYM sj139023d62 20 22 templates template NOUN sj139023d62 20 23 for for ADP sj139023d62 20 24 controlled control VERB sj139023d62 20 25 materials material NOUN sj139023d62 20 26 synthesis synthesis NOUN sj139023d62 20 27 . . PUNCT sj139023d62 21 1 the the DET sj139023d62 21 2 three three NUM sj139023d62 21 3 representative representative ADJ sj139023d62 21 4 examples example NOUN sj139023d62 21 5 indicate indicate VERB sj139023d62 21 6 that that SCONJ sj139023d62 21 7 pipetting pipette VERB sj139023d62 21 8 uniform uniform NOUN sj139023d62 21 9 droplets droplet NOUN sj139023d62 21 10 and and CCONJ sj139023d62 21 11 related related ADJ sj139023d62 21 12 techniques technique NOUN sj139023d62 21 13 are be AUX sj139023d62 21 14 perfect perfect ADJ sj139023d62 21 15 for for ADP sj139023d62 21 16 droplet droplet ADJ sj139023d62 21 17 microfluidics microfluidic NOUN sj139023d62 21 18 applications application NOUN sj139023d62 21 19 at at ADP sj139023d62 21 20 the the DET sj139023d62 21 21 point point NOUN sj139023d62 21 22 of of ADP sj139023d62 21 23 need need NOUN sj139023d62 21 24 . . PUNCT sj139023d62 22 1 in in ADP sj139023d62 22 2 chapter chapter NOUN sj139023d62 22 3 5 5 NUM sj139023d62 22 4 , , PUNCT sj139023d62 22 5 further further ADJ sj139023d62 22 6 efforts effort NOUN sj139023d62 22 7 are be AUX sj139023d62 22 8 made make VERB sj139023d62 22 9 to to PART sj139023d62 22 10 upgrade upgrade VERB sj139023d62 22 11 the the DET sj139023d62 22 12 pipette pipette NOUN sj139023d62 22 13 droplet droplet PROPN sj139023d62 22 14 microfluidics microfluidics PROPN sj139023d62 22 15 . . PUNCT sj139023d62 23 1 though though SCONJ sj139023d62 23 2 simple simple ADJ sj139023d62 23 3 manual manual NOUN sj139023d62 23 4 pipetting pipette VERB sj139023d62 23 5 works work NOUN sj139023d62 23 6 for for ADP sj139023d62 23 7 uniform uniform ADJ sj139023d62 23 8 droplet droplet ADJ sj139023d62 23 9 generation generation NOUN sj139023d62 23 10 , , PUNCT sj139023d62 23 11 automated automate VERB sj139023d62 23 12 pipetting pipetting NOUN sj139023d62 23 13 is be AUX sj139023d62 23 14 more more ADV sj139023d62 23 15 ergonomic ergonomic ADJ sj139023d62 23 16 - - PUNCT sj139023d62 23 17 friendly friendly ADJ sj139023d62 23 18 and and CCONJ sj139023d62 23 19 suitable suitable ADJ sj139023d62 23 20 for for ADP sj139023d62 23 21 high high ADJ sj139023d62 23 22 throughput throughput NOUN sj139023d62 23 23 parallel parallel ADJ sj139023d62 23 24 droplet droplet ADJ sj139023d62 23 25 generation generation NOUN sj139023d62 23 26 . . PUNCT sj139023d62 24 1 an an DET sj139023d62 24 2 open open ADJ sj139023d62 24 3 - - PUNCT sj139023d62 24 4 source source NOUN sj139023d62 24 5 stepper stepper NOUN sj139023d62 24 6 - - PUNCT sj139023d62 24 7 motor motor NOUN sj139023d62 24 8 - - PUNCT sj139023d62 24 9 based base VERB sj139023d62 24 10 device device NOUN sj139023d62 24 11 is be AUX sj139023d62 24 12 assembled assemble VERB sj139023d62 24 13 and and CCONJ sj139023d62 24 14 programmed program VERB sj139023d62 24 15 to to PART sj139023d62 24 16 automatically automatically ADV sj139023d62 24 17 pipette pipette VERB sj139023d62 24 18 liquids liquid NOUN sj139023d62 24 19 into into ADP sj139023d62 24 20 uniform uniform NOUN sj139023d62 24 21 droplets droplet VERB sj139023d62 24 22 via via ADP sj139023d62 24 23 the the DET sj139023d62 24 24 elliptical elliptical ADJ sj139023d62 24 25 - - PUNCT sj139023d62 24 26 orifice orifice NOUN sj139023d62 24 27 pipette pipette NOUN sj139023d62 24 28 tips tip NOUN sj139023d62 24 29 . . PUNCT sj139023d62 25 1 in in ADP sj139023d62 25 2 addition addition NOUN sj139023d62 25 3 to to ADP sj139023d62 25 4 the the DET sj139023d62 25 5 upgrade upgrade NOUN sj139023d62 25 6 of of ADP sj139023d62 25 7 droplet droplet ADJ sj139023d62 25 8 generation generation NOUN sj139023d62 25 9 , , PUNCT sj139023d62 25 10 droplets droplet NOUN sj139023d62 25 11 are be AUX sj139023d62 25 12 used use VERB sj139023d62 25 13 in in ADP sj139023d62 25 14 duplex duplex NOUN sj139023d62 25 15 / / SYM sj139023d62 25 16 multiplex multiplex ADJ sj139023d62 25 17 modes mode NOUN sj139023d62 25 18 to to PART sj139023d62 25 19 be be AUX sj139023d62 25 20 more more ADV sj139023d62 25 21 powerful powerful ADJ sj139023d62 25 22 . . PUNCT sj139023d62 26 1 duplex duplex NOUN sj139023d62 26 2 ddpcr ddpcr NOUN sj139023d62 26 3 is be AUX sj139023d62 26 4 conducted conduct VERB sj139023d62 26 5 for for ADP sj139023d62 26 6 accurate accurate ADJ sj139023d62 26 7 dna dna NOUN sj139023d62 26 8 methylation methylation NOUN sj139023d62 26 9 analysis analysis NOUN sj139023d62 26 10 and and CCONJ sj139023d62 26 11 multiplex multiplex ADJ sj139023d62 26 12 ddpcr ddpcr NOUN sj139023d62 26 13 is be AUX sj139023d62 26 14 validated validate VERB sj139023d62 26 15 for for ADP sj139023d62 26 16 zika zika X sj139023d62 26 17 / / SYM sj139023d62 26 18 dengue dengue PROPN sj139023d62 26 19 / / SYM sj139023d62 26 20 chikungunya chikungunya PROPN sj139023d62 26 21 multi multi PROPN sj139023d62 26 22 - - NOUN sj139023d62 26 23 virus virus ADJ sj139023d62 26 24 rnas rnas PROPN sj139023d62 26 25 test test NOUN sj139023d62 26 26 . . PUNCT sj139023d62 27 1 moreover moreover ADV sj139023d62 27 2 , , PUNCT sj139023d62 27 3 dual dual ADJ sj139023d62 27 4 - - PUNCT sj139023d62 27 5 droplet droplet ADJ sj139023d62 27 6 systems system NOUN sj139023d62 27 7 of of ADP sj139023d62 27 8 two two NUM sj139023d62 27 9 types type NOUN sj139023d62 27 10 of of ADP sj139023d62 27 11 droplet droplet ADJ sj139023d62 27 12 sizes size NOUN sj139023d62 27 13 are be AUX sj139023d62 27 14 used use VERB sj139023d62 27 15 for for ADP sj139023d62 27 16 the the DET sj139023d62 27 17 investigation investigation NOUN sj139023d62 27 18 of of ADP sj139023d62 27 19 controlled control VERB sj139023d62 27 20 reactions reaction NOUN sj139023d62 27 21 in in ADP sj139023d62 27 22 droplets droplet NOUN sj139023d62 27 23 . . PUNCT sj139023d62 28 1 therefore therefore ADV sj139023d62 28 2 , , PUNCT sj139023d62 28 3 comprehensive comprehensive ADJ sj139023d62 28 4 technologies technology NOUN sj139023d62 28 5 are be AUX sj139023d62 28 6 invented invent VERB sj139023d62 28 7 , , PUNCT sj139023d62 28 8 engineered engineer VERB sj139023d62 28 9 , , PUNCT sj139023d62 28 10 optimized optimize VERB sj139023d62 28 11 , , PUNCT sj139023d62 28 12 and and CCONJ sj139023d62 28 13 upgraded upgrade VERB sj139023d62 28 14 for for ADP sj139023d62 28 15 conducting conduct VERB sj139023d62 28 16 droplet droplet ADJ sj139023d62 28 17 microfluidics microfluidic NOUN sj139023d62 28 18 applications application NOUN sj139023d62 28 19 at at ADP sj139023d62 28 20 the the DET sj139023d62 28 21 point point NOUN sj139023d62 28 22 of of ADP sj139023d62 28 23 need need NOUN sj139023d62 28 24 / / SYM sj139023d62 28 25 use use NOUN sj139023d62 28 26 / / SYM sj139023d62 28 27 care care NOUN sj139023d62 28 28 . . PUNCT