id sid tid token lemma pos s4655d8937g 1 1 the the DET s4655d8937g 1 2 chlorooxide chlorooxide ADJ s4655d8937g 1 3 anions anion NOUN s4655d8937g 1 4 form form VERB s4655d8937g 1 5 a a DET s4655d8937g 1 6 class class NOUN s4655d8937g 1 7 of of ADP s4655d8937g 1 8 highly highly ADV s4655d8937g 1 9 water water NOUN s4655d8937g 1 10 - - PUNCT s4655d8937g 1 11 soluble soluble ADJ s4655d8937g 1 12 , , PUNCT s4655d8937g 1 13 toxic toxic ADJ s4655d8937g 1 14 species specie NOUN s4655d8937g 1 15 . . PUNCT s4655d8937g 2 1 perchlorate perchlorate NOUN s4655d8937g 2 2 reductase reductase NOUN s4655d8937g 2 3 from from ADP s4655d8937g 2 4 dechloromonas dechloromonas PROPN s4655d8937g 2 5 aromatica aromatica PROPN s4655d8937g 2 6 rcb rcb PROPN s4655d8937g 2 7 is be AUX s4655d8937g 2 8 responsible responsible ADJ s4655d8937g 2 9 for for ADP s4655d8937g 2 10 the the DET s4655d8937g 2 11 enzymatic enzymatic ADJ s4655d8937g 2 12 reduction reduction NOUN s4655d8937g 2 13 of of ADP s4655d8937g 2 14 perchlorate perchlorate NOUN s4655d8937g 2 15 to to ADP s4655d8937g 2 16 chlorate chlorate NOUN s4655d8937g 2 17 and and CCONJ s4655d8937g 2 18 chlorate chlorate ADJ s4655d8937g 2 19 to to PART s4655d8937g 2 20 chlorite chlorite VERB s4655d8937g 2 21 in in ADP s4655d8937g 2 22 two two NUM s4655d8937g 2 23 , , PUNCT s4655d8937g 2 24 two two NUM s4655d8937g 2 25 electron electron NOUN s4655d8937g 2 26 transfers transfer NOUN s4655d8937g 2 27 . . PUNCT s4655d8937g 3 1 two two NUM s4655d8937g 3 2 heterologous heterologous ADJ s4655d8937g 3 3 expression expression NOUN s4655d8937g 3 4 systems system NOUN s4655d8937g 3 5 for for ADP s4655d8937g 3 6 perchlorate perchlorate NOUN s4655d8937g 3 7 reductase reductase NOUN s4655d8937g 3 8 were be AUX s4655d8937g 3 9 constructed construct VERB s4655d8937g 3 10 in in ADP s4655d8937g 3 11 this this DET s4655d8937g 3 12 work work NOUN s4655d8937g 3 13 . . PUNCT s4655d8937g 4 1 one one NUM s4655d8937g 4 2 system system NOUN s4655d8937g 4 3 was be AUX s4655d8937g 4 4 based base VERB s4655d8937g 4 5 on on ADP s4655d8937g 4 6 the the DET s4655d8937g 4 7 expression expression NOUN s4655d8937g 4 8 of of ADP s4655d8937g 4 9 active active ADJ s4655d8937g 4 10 nitrate nitrate NOUN s4655d8937g 4 11 reductase reductase NOUN s4655d8937g 4 12 and and CCONJ s4655d8937g 4 13 the the DET s4655d8937g 4 14 second second ADJ s4655d8937g 4 15 system system NOUN s4655d8937g 4 16 utilized utilize VERB s4655d8937g 4 17 polycistronic polycistronic ADJ s4655d8937g 4 18 coexpression coexpression NOUN s4655d8937g 4 19 . . PUNCT s4655d8937g 5 1 gel gel PROPN s4655d8937g 5 2 documentation documentation NOUN s4655d8937g 5 3 of of ADP s4655d8937g 5 4 enzymatic enzymatic ADJ s4655d8937g 5 5 digests digest NOUN s4655d8937g 5 6 indicated indicate VERB s4655d8937g 5 7 successful successful ADJ s4655d8937g 5 8 molecular molecular ADJ s4655d8937g 5 9 subcloning subcloning NOUN s4655d8937g 5 10 of of ADP s4655d8937g 5 11 both both DET s4655d8937g 5 12 constructs construct NOUN s4655d8937g 5 13 . . PUNCT s4655d8937g 6 1 however however ADV s4655d8937g 6 2 , , PUNCT s4655d8937g 6 3 the the DET s4655d8937g 6 4 lack lack NOUN s4655d8937g 6 5 of of ADP s4655d8937g 6 6 iptg iptg NOUN s4655d8937g 6 7 - - PUNCT s4655d8937g 6 8 inducible inducible VERB s4655d8937g 6 9 overexpression overexpression NOUN s4655d8937g 6 10 of of ADP s4655d8937g 6 11 the the DET s4655d8937g 6 12 above above ADJ s4655d8937g 6 13 constructs construct NOUN s4655d8937g 6 14 supported support VERB s4655d8937g 6 15 the the DET s4655d8937g 6 16 supposition supposition NOUN s4655d8937g 6 17 that that SCONJ s4655d8937g 6 18 the the DET s4655d8937g 6 19 presence presence NOUN s4655d8937g 6 20 of of ADP s4655d8937g 6 21 several several ADJ s4655d8937g 6 22 rare rare ADJ s4655d8937g 6 23 codons codon NOUN s4655d8937g 6 24 halted halt VERB s4655d8937g 6 25 dna dna NOUN s4655d8937g 6 26 replication replication NOUN s4655d8937g 6 27 . . PUNCT s4655d8937g 7 1 this this DET s4655d8937g 7 2 finding finding NOUN s4655d8937g 7 3 suggested suggest VERB s4655d8937g 7 4 the the DET s4655d8937g 7 5 use use NOUN s4655d8937g 7 6 of of ADP s4655d8937g 7 7 an an DET s4655d8937g 7 8 e e NOUN s4655d8937g 7 9 .coli .coli NOUN s4655d8937g 7 10 strain strain NOUN s4655d8937g 7 11 genetically genetically ADV s4655d8937g 7 12 modified modify VERB s4655d8937g 7 13 to to PART s4655d8937g 7 14 replicate replicate VERB s4655d8937g 7 15 such such ADJ s4655d8937g 7 16 rare rare ADJ s4655d8937g 7 17 codons codon NOUN s4655d8937g 7 18 is be AUX s4655d8937g 7 19 necessary necessary ADJ s4655d8937g 7 20 for for SCONJ s4655d8937g 7 21 the the DET s4655d8937g 7 22 desired desire VERB s4655d8937g 7 23 overexpression overexpression NOUN s4655d8937g 7 24 to to PART s4655d8937g 7 25 occur occur VERB s4655d8937g 7 26 . . PUNCT s4655d8937g 8 1 the the DET s4655d8937g 8 2 experimentally experimentally ADV s4655d8937g 8 3 confirmed confirm VERB s4655d8937g 8 4 high high ADJ s4655d8937g 8 5 isoelectic isoelectic ADJ s4655d8937g 8 6 point point NOUN s4655d8937g 8 7 of of ADP s4655d8937g 8 8 said say VERB s4655d8937g 8 9 enzyme enzyme NOUN s4655d8937g 8 10 ( ( PUNCT s4655d8937g 8 11 8.20 8.20 NUM s4655d8937g 8 12 - - SYM s4655d8937g 8 13 9.6 9.6 NUM s4655d8937g 8 14 ) ) PUNCT s4655d8937g 8 15 led lead VERB s4655d8937g 8 16 to to ADP s4655d8937g 8 17 the the DET s4655d8937g 8 18 construction construction NOUN s4655d8937g 8 19 of of ADP s4655d8937g 8 20 a a DET s4655d8937g 8 21 three three NUM s4655d8937g 8 22 - - PUNCT s4655d8937g 8 23 part part NOUN s4655d8937g 8 24 purification purification NOUN s4655d8937g 8 25 scheme scheme NOUN s4655d8937g 8 26 . . PUNCT s4655d8937g 9 1 utilizing utilize VERB s4655d8937g 9 2 deae deae PROPN s4655d8937g 9 3 ( ( PUNCT s4655d8937g 9 4 diethylaminoethyl diethylaminoethyl PROPN s4655d8937g 9 5 ) ) PUNCT s4655d8937g 9 6 reverse reverse ADJ s4655d8937g 9 7 anion anion NOUN s4655d8937g 9 8 exchange exchange NOUN s4655d8937g 9 9 as as ADP s4655d8937g 9 10 a a DET s4655d8937g 9 11 first first ADJ s4655d8937g 9 12 step step NOUN s4655d8937g 9 13 resulted result VERB s4655d8937g 9 14 in in ADP s4655d8937g 9 15 an an DET s4655d8937g 9 16 increase increase NOUN s4655d8937g 9 17 of of ADP s4655d8937g 9 18 perchlorate perchlorate NOUN s4655d8937g 9 19 reductase reductase NOUN s4655d8937g 9 20 specific specific ADJ s4655d8937g 9 21 activity activity NOUN s4655d8937g 9 22 from from ADP s4655d8937g 9 23 0.0759 0.0759 NUM s4655d8937g 9 24 u u NOUN s4655d8937g 9 25 / / SYM s4655d8937g 9 26 mg mg NOUN s4655d8937g 9 27 to to ADP s4655d8937g 9 28 0.218 0.218 NUM s4655d8937g 9 29 u u NOUN s4655d8937g 9 30 / / SYM s4655d8937g 9 31 mg mg NOUN s4655d8937g 9 32 . . PUNCT s4655d8937g 10 1 while while SCONJ s4655d8937g 10 2 further further ADJ s4655d8937g 10 3 downstream downstream ADJ s4655d8937g 10 4 steps step NOUN s4655d8937g 10 5 ( ( PUNCT s4655d8937g 10 6 carboxymethyl carboxymethyl NOUN s4655d8937g 10 7 ( ( PUNCT s4655d8937g 10 8 cm cm PROPN s4655d8937g 10 9 ) ) PUNCT s4655d8937g 10 10 sepharose sepharose VERB s4655d8937g 10 11 fast fast PROPN s4655d8937g 10 12 flow flow NOUN s4655d8937g 10 13 and and CCONJ s4655d8937g 10 14 sephacryl sephacryl VERB s4655d8937g 10 15 s-200 s-200 PRON s4655d8937g 10 16 high high ADJ s4655d8937g 10 17 resolution resolution NOUN s4655d8937g 10 18 gel gel NOUN s4655d8937g 10 19 filtration filtration NOUN s4655d8937g 10 20 ) ) PUNCT s4655d8937g 10 21 were be AUX s4655d8937g 10 22 able able ADJ s4655d8937g 10 23 to to PART s4655d8937g 10 24 increase increase VERB s4655d8937g 10 25 specific specific ADJ s4655d8937g 10 26 activity activity NOUN s4655d8937g 10 27 to to ADP s4655d8937g 10 28 2.29 2.29 NUM s4655d8937g 10 29 u u PROPN s4655d8937g 10 30 / / SYM s4655d8937g 10 31 mg mg NOUN s4655d8937g 10 32 , , PUNCT s4655d8937g 10 33 total total ADJ s4655d8937g 10 34 activity activity NOUN s4655d8937g 10 35 decreased decrease VERB s4655d8937g 10 36 over over ADP s4655d8937g 10 37 the the DET s4655d8937g 10 38 course course NOUN s4655d8937g 10 39 of of ADP s4655d8937g 10 40 purification purification NOUN s4655d8937g 10 41 and and CCONJ s4655d8937g 10 42 complete complete ADJ s4655d8937g 10 43 separation separation NOUN s4655d8937g 10 44 from from ADP s4655d8937g 10 45 cellular cellular ADJ s4655d8937g 10 46 proteins protein NOUN s4655d8937g 10 47 ( ( PUNCT s4655d8937g 10 48 mainly mainly ADV s4655d8937g 10 49 chlorite chlorite NOUN s4655d8937g 10 50 dismutase dismutase NOUN s4655d8937g 10 51 ) ) PUNCT s4655d8937g 10 52 was be AUX s4655d8937g 10 53 not not PART s4655d8937g 10 54 achieved achieve VERB s4655d8937g 10 55 . . PUNCT s4655d8937g 11 1 thus thus ADV s4655d8937g 11 2 , , PUNCT s4655d8937g 11 3 a a DET s4655d8937g 11 4 summary summary NOUN s4655d8937g 11 5 of of ADP s4655d8937g 11 6 the the DET s4655d8937g 11 7 partial partial ADJ s4655d8937g 11 8 purification purification NOUN s4655d8937g 11 9 achieved achieve VERB s4655d8937g 11 10 is be AUX s4655d8937g 11 11 presented present VERB s4655d8937g 11 12 rather rather ADV s4655d8937g 11 13 than than ADP s4655d8937g 11 14 the the DET s4655d8937g 11 15 complete complete ADJ s4655d8937g 11 16 purification purification NOUN s4655d8937g 11 17 of of ADP s4655d8937g 11 18 perchlorate perchlorate NOUN s4655d8937g 11 19 reductase reductase NOUN s4655d8937g 11 20 from from ADP s4655d8937g 11 21 d. d. PROPN s4655d8937g 11 22 aromtica aromtica PROPN s4655d8937g 11 23 . . PUNCT