id sid tid token lemma pos pk02c824z19 1 1 sortases sortases PROPN pk02c824z19 1 2 play play VERB pk02c824z19 1 3 important important ADJ pk02c824z19 1 4 roles role NOUN pk02c824z19 1 5 in in ADP pk02c824z19 1 6 covalently covalently ADV pk02c824z19 1 7 anchoring anchor VERB pk02c824z19 1 8 bacterial bacterial ADJ pk02c824z19 1 9 virulence virulence NOUN pk02c824z19 1 10 factors factor NOUN pk02c824z19 1 11 to to ADP pk02c824z19 1 12 the the DET pk02c824z19 1 13 cell cell NOUN pk02c824z19 1 14 wall wall NOUN pk02c824z19 1 15 . . PUNCT pk02c824z19 2 1 sortase sortase PROPN pk02c824z19 2 2 a a DET pk02c824z19 2 3 ( ( PUNCT pk02c824z19 2 4 srta srta PROPN pk02c824z19 2 5 ) ) PUNCT pk02c824z19 2 6 , , PUNCT pk02c824z19 2 7 the the DET pk02c824z19 2 8 most most ADV pk02c824z19 2 9 ubiquitous ubiquitous ADJ pk02c824z19 2 10 sortase sortase NOUN pk02c824z19 2 11 in in ADP pk02c824z19 2 12 gram gram ADJ pk02c824z19 2 13 - - PUNCT pk02c824z19 2 14 positive positive ADJ pk02c824z19 2 15 bacteria bacteria NOUN pk02c824z19 2 16 , , PUNCT pk02c824z19 2 17 is be AUX pk02c824z19 2 18 regarded regard VERB pk02c824z19 2 19 as as ADP pk02c824z19 2 20 ' ' PUNCT pk02c824z19 2 21 the the DET pk02c824z19 2 22 housekeeping housekeeping NOUN pk02c824z19 2 23 sortase sortase NOUN pk02c824z19 2 24 ' ' PUNCT pk02c824z19 2 25 due due ADP pk02c824z19 2 26 to to ADP pk02c824z19 2 27 its its PRON pk02c824z19 2 28 role role NOUN pk02c824z19 2 29 in in ADP pk02c824z19 2 30 anchoring anchor VERB pk02c824z19 2 31 many many ADJ pk02c824z19 2 32 proteins protein NOUN pk02c824z19 2 33 involved involve VERB pk02c824z19 2 34 in in ADP pk02c824z19 2 35 adhesion adhesion NOUN pk02c824z19 2 36 , , PUNCT pk02c824z19 2 37 immune immune ADJ pk02c824z19 2 38 invasion invasion NOUN pk02c824z19 2 39 , , PUNCT pk02c824z19 2 40 internalization internalization NOUN pk02c824z19 2 41 , , PUNCT pk02c824z19 2 42 and and CCONJ pk02c824z19 2 43 phage phage NOUN pk02c824z19 2 44 recognition recognition NOUN pk02c824z19 2 45 . . PUNCT pk02c824z19 3 1 the the DET pk02c824z19 3 2 activity activity NOUN pk02c824z19 3 3 of of ADP pk02c824z19 3 4 srta srta PROPN pk02c824z19 3 5 is be AUX pk02c824z19 3 6 specific specific ADJ pk02c824z19 3 7 to to ADP pk02c824z19 3 8 cell cell NOUN pk02c824z19 3 9 surface surface NOUN pk02c824z19 3 10 proteins protein NOUN pk02c824z19 3 11 with with ADP pk02c824z19 3 12 a a DET pk02c824z19 3 13 c c X pk02c824z19 3 14 - - PUNCT pk02c824z19 3 15 terminal terminal ADJ pk02c824z19 3 16 lpxtg lpxtg PROPN pk02c824z19 3 17 - - PUNCT pk02c824z19 3 18 motif motif NOUN pk02c824z19 3 19 followed follow VERB pk02c824z19 3 20 by by ADP pk02c824z19 3 21 a a DET pk02c824z19 3 22 hydrophobic hydrophobic NOUN pk02c824z19 3 23 region region NOUN pk02c824z19 3 24 and and CCONJ pk02c824z19 3 25 a a DET pk02c824z19 3 26 positively positively ADV pk02c824z19 3 27 charged charge VERB pk02c824z19 3 28 tail tail NOUN pk02c824z19 3 29 . . PUNCT pk02c824z19 4 1 many many ADJ pk02c824z19 4 2 of of ADP pk02c824z19 4 3 the the DET pk02c824z19 4 4 cell cell NOUN pk02c824z19 4 5 surface surface NOUN pk02c824z19 4 6 virulence virulence NOUN pk02c824z19 4 7 factors factor NOUN pk02c824z19 4 8 in in ADP pk02c824z19 4 9 group group NOUN pk02c824z19 4 10 a a DET pk02c824z19 4 11 streptococcus streptococcus NOUN pk02c824z19 4 12 pyogenes pyogene NOUN pk02c824z19 4 13 ( ( PUNCT pk02c824z19 4 14 gas gas NOUN pk02c824z19 4 15 ) ) PUNCT pk02c824z19 4 16 , , PUNCT pk02c824z19 4 17 including include VERB pk02c824z19 4 18 plasminogen plasminogen NOUN pk02c824z19 4 19 - - PUNCT pk02c824z19 4 20 binding bind VERB pk02c824z19 4 21 m m NOUN pk02c824z19 4 22 - - PUNCT pk02c824z19 4 23 protein protein NOUN pk02c824z19 4 24 ( ( PUNCT pk02c824z19 4 25 pam pam PROPN pk02c824z19 4 26 ) ) PUNCT pk02c824z19 4 27 , , PUNCT pk02c824z19 4 28 have have VERB pk02c824z19 4 29 a a DET pk02c824z19 4 30 lpxtg lpxtg ADJ pk02c824z19 4 31 - - PUNCT pk02c824z19 4 32 motif motif NOUN pk02c824z19 4 33 and and CCONJ pk02c824z19 4 34 are be AUX pk02c824z19 4 35 anchored anchor VERB pk02c824z19 4 36 to to ADP pk02c824z19 4 37 the the DET pk02c824z19 4 38 cell cell NOUN pk02c824z19 4 39 wall wall NOUN pk02c824z19 4 40 by by ADP pk02c824z19 4 41 srta srta PROPN pk02c824z19 4 42 . . PUNCT pk02c824z19 5 1 while while SCONJ pk02c824z19 5 2 the the DET pk02c824z19 5 3 role role NOUN pk02c824z19 5 4 of of ADP pk02c824z19 5 5 srta srta NOUN pk02c824z19 5 6 in in ADP pk02c824z19 5 7 the the DET pk02c824z19 5 8 presentation presentation NOUN pk02c824z19 5 9 of of ADP pk02c824z19 5 10 virulence virulence NOUN pk02c824z19 5 11 factors factor NOUN pk02c824z19 5 12 on on ADP pk02c824z19 5 13 the the DET pk02c824z19 5 14 cell cell NOUN pk02c824z19 5 15 wall wall NOUN pk02c824z19 5 16 has have AUX pk02c824z19 5 17 been be AUX pk02c824z19 5 18 recognized recognize VERB pk02c824z19 5 19 , , PUNCT pk02c824z19 5 20 the the DET pk02c824z19 5 21 ability ability NOUN pk02c824z19 5 22 of of ADP pk02c824z19 5 23 these these DET pk02c824z19 5 24 virulence virulence NOUN pk02c824z19 5 25 factors factor NOUN pk02c824z19 5 26 to to PART pk02c824z19 5 27 present present VERB pk02c824z19 5 28 themselves themselves PRON pk02c824z19 5 29 and and CCONJ pk02c824z19 5 30 maintain maintain VERB pk02c824z19 5 31 function function NOUN pk02c824z19 5 32 without without ADP pk02c824z19 5 33 srta srta PROPN pk02c824z19 5 34 has have AUX pk02c824z19 5 35 not not PART pk02c824z19 5 36 been be AUX pk02c824z19 5 37 well well ADV pk02c824z19 5 38 studied study VERB pk02c824z19 5 39 . . PUNCT pk02c824z19 6 1 the the DET pk02c824z19 6 2 goal goal NOUN pk02c824z19 6 3 of of ADP pk02c824z19 6 4 this this DET pk02c824z19 6 5 research research NOUN pk02c824z19 6 6 is be AUX pk02c824z19 6 7 to to PART pk02c824z19 6 8 investigate investigate VERB pk02c824z19 6 9 how how SCONJ pk02c824z19 6 10 lpxtg lpxtg PROPN pk02c824z19 6 11 - - PUNCT pk02c824z19 6 12 motif motif NOUN pk02c824z19 6 13 surface surface NOUN pk02c824z19 6 14 proteins protein NOUN pk02c824z19 6 15 anchor anchor NOUN pk02c824z19 6 16 and and CCONJ pk02c824z19 6 17 present present VERB pk02c824z19 6 18 themselves themselves PRON pk02c824z19 6 19 without without ADP pk02c824z19 6 20 srta srta PROPN pk02c824z19 6 21 , , PUNCT pk02c824z19 6 22 what what DET pk02c824z19 6 23 effects effect NOUN pk02c824z19 6 24 this this PRON pk02c824z19 6 25 has have VERB pk02c824z19 6 26 on on ADP pk02c824z19 6 27 their their PRON pk02c824z19 6 28 function function NOUN pk02c824z19 6 29 , , PUNCT pk02c824z19 6 30 and and CCONJ pk02c824z19 6 31 what what PRON pk02c824z19 6 32 subsequent subsequent ADJ pk02c824z19 6 33 effects effect NOUN pk02c824z19 6 34 these these DET pk02c824z19 6 35 changes change NOUN pk02c824z19 6 36 have have VERB pk02c824z19 6 37 on on ADP pk02c824z19 6 38 cell cell NOUN pk02c824z19 6 39 hardiness hardiness NOUN pk02c824z19 6 40 and and CCONJ pk02c824z19 6 41 virulence.a virulence.a ADJ pk02c824z19 6 42 gene gene NOUN pk02c824z19 6 43 deletion deletion NOUN pk02c824z19 6 44 mutant mutant NOUN pk02c824z19 6 45 for for ADP pk02c824z19 6 46 srta srta PROPN pk02c824z19 6 47 was be AUX pk02c824z19 6 48 made make VERB pk02c824z19 6 49 in in ADP pk02c824z19 6 50 gas gas NOUN pk02c824z19 6 51 strain strain NOUN pk02c824z19 6 52 ap53 ap53 PROPN pk02c824z19 6 53 and and CCONJ pk02c824z19 6 54 compared compare VERB pk02c824z19 6 55 with with ADP pk02c824z19 6 56 the the DET pk02c824z19 6 57 parent parent NOUN pk02c824z19 6 58 strain strain NOUN pk02c824z19 6 59 for for ADP pk02c824z19 6 60 the the DET pk02c824z19 6 61 investigations investigation NOUN pk02c824z19 6 62 described describe VERB pk02c824z19 6 63 . . PUNCT pk02c824z19 7 1 the the DET pk02c824z19 7 2 cell cell NOUN pk02c824z19 7 3 wall wall NOUN pk02c824z19 7 4 and and CCONJ pk02c824z19 7 5 cell cell NOUN pk02c824z19 7 6 membrane membrane NOUN pk02c824z19 7 7 were be AUX pk02c824z19 7 8 isolated isolate VERB pk02c824z19 7 9 via via ADP pk02c824z19 7 10 phage phage NOUN pk02c824z19 7 11 lysin lysin PROPN pk02c824z19 7 12 c c PROPN pk02c824z19 7 13 ( ( PUNCT pk02c824z19 7 14 plyc plyc PROPN pk02c824z19 7 15 ) ) PUNCT pk02c824z19 7 16 and and CCONJ pk02c824z19 7 17 centrifugation centrifugation NOUN pk02c824z19 7 18 and and CCONJ pk02c824z19 7 19 examined examine VERB pk02c824z19 7 20 for for ADP pk02c824z19 7 21 pam pam PROPN pk02c824z19 7 22 content content NOUN pk02c824z19 7 23 by by ADP pk02c824z19 7 24 western western ADJ pk02c824z19 7 25 blotting blotting NOUN pk02c824z19 7 26 . . PUNCT pk02c824z19 8 1 whole whole ADJ pk02c824z19 8 2 cell cell NOUN pk02c824z19 8 3 elisas elisa NOUN pk02c824z19 8 4 were be AUX pk02c824z19 8 5 also also ADV pk02c824z19 8 6 employed employ VERB pk02c824z19 8 7 to to PART pk02c824z19 8 8 investigate investigate VERB pk02c824z19 8 9 the the DET pk02c824z19 8 10 presence presence NOUN pk02c824z19 8 11 and and CCONJ pk02c824z19 8 12 presentation presentation NOUN pk02c824z19 8 13 of of ADP pk02c824z19 8 14 proteins protein NOUN pk02c824z19 8 15 on on ADP pk02c824z19 8 16 the the DET pk02c824z19 8 17 cell cell NOUN pk02c824z19 8 18 surface surface NOUN pk02c824z19 8 19 . . PUNCT pk02c824z19 9 1 the the DET pk02c824z19 9 2 results result NOUN pk02c824z19 9 3 obtained obtain VERB pk02c824z19 9 4 from from ADP pk02c824z19 9 5 these these DET pk02c824z19 9 6 assays assay NOUN pk02c824z19 9 7 demonstrate demonstrate VERB pk02c824z19 9 8 in in ADP pk02c824z19 9 9 the the DET pk02c824z19 9 10 δsrta δsrta NOUN pk02c824z19 9 11 mutant mutant VERB pk02c824z19 9 12 the the DET pk02c824z19 9 13 retention retention NOUN pk02c824z19 9 14 of of ADP pk02c824z19 9 15 lpxtg lpxtg PROPN pk02c824z19 9 16 - - PUNCT pk02c824z19 9 17 motif motif NOUN pk02c824z19 9 18 proteins protein NOUN pk02c824z19 9 19 in in ADP pk02c824z19 9 20 the the DET pk02c824z19 9 21 cell cell NOUN pk02c824z19 9 22 membrane membrane NOUN pk02c824z19 9 23 and and CCONJ pk02c824z19 9 24 that that SCONJ pk02c824z19 9 25 the the DET pk02c824z19 9 26 presentation presentation NOUN pk02c824z19 9 27 of of ADP pk02c824z19 9 28 these these DET pk02c824z19 9 29 proteins protein NOUN pk02c824z19 9 30 to to ADP pk02c824z19 9 31 the the DET pk02c824z19 9 32 environment environment NOUN pk02c824z19 9 33 remains remain VERB pk02c824z19 9 34 unaltered unaltered ADJ pk02c824z19 9 35 . . PUNCT pk02c824z19 10 1 the the DET pk02c824z19 10 2 functionality functionality NOUN pk02c824z19 10 3 of of ADP pk02c824z19 10 4 these these DET pk02c824z19 10 5 proteins protein NOUN pk02c824z19 10 6 was be AUX pk02c824z19 10 7 investigated investigate VERB pk02c824z19 10 8 via via ADP pk02c824z19 10 9 the the DET pk02c824z19 10 10 ability ability NOUN pk02c824z19 10 11 of of ADP pk02c824z19 10 12 the the DET pk02c824z19 10 13 cells cell NOUN pk02c824z19 10 14 to to PART pk02c824z19 10 15 bind bind VERB pk02c824z19 10 16 and and CCONJ pk02c824z19 10 17 activate activate VERB pk02c824z19 10 18 human human ADJ pk02c824z19 10 19 plasminogen plasminogen NOUN pk02c824z19 10 20 ( ( PUNCT pk02c824z19 10 21 hpg hpg PROPN pk02c824z19 10 22 ) ) PUNCT pk02c824z19 10 23 . . PUNCT pk02c824z19 11 1 in in ADP pk02c824z19 11 2 the the DET pk02c824z19 11 3 absence absence NOUN pk02c824z19 11 4 of of ADP pk02c824z19 11 5 srta srta PROPN pk02c824z19 11 6 , , PUNCT pk02c824z19 11 7 the the DET pk02c824z19 11 8 cells cell NOUN pk02c824z19 11 9 were be AUX pk02c824z19 11 10 found find VERB pk02c824z19 11 11 to to PART pk02c824z19 11 12 retain retain VERB pk02c824z19 11 13 their their PRON pk02c824z19 11 14 ability ability NOUN pk02c824z19 11 15 to to PART pk02c824z19 11 16 bind bind VERB pk02c824z19 11 17 hpg hpg PROPN pk02c824z19 11 18 via via ADP pk02c824z19 11 19 pam pam PROPN pk02c824z19 11 20 and and CCONJ pk02c824z19 11 21 activate activate VERB pk02c824z19 11 22 hpg hpg NOUN pk02c824z19 11 23 in in ADP pk02c824z19 11 24 the the DET pk02c824z19 11 25 presence presence NOUN pk02c824z19 11 26 of of ADP pk02c824z19 11 27 the the DET pk02c824z19 11 28 secreted secrete VERB pk02c824z19 11 29 virulence virulence NOUN pk02c824z19 11 30 factor factor NOUN pk02c824z19 11 31 streptokinase streptokinase PROPN pk02c824z19 11 32 . . PUNCT pk02c824z19 12 1 the the DET pk02c824z19 12 2 viability viability NOUN pk02c824z19 12 3 and and CCONJ pk02c824z19 12 4 environmental environmental ADJ pk02c824z19 12 5 hardiness hardiness NOUN pk02c824z19 12 6 of of ADP pk02c824z19 12 7 the the DET pk02c824z19 12 8 cells cell NOUN pk02c824z19 12 9 were be AUX pk02c824z19 12 10 investigated investigate VERB pk02c824z19 12 11 via via ADP pk02c824z19 12 12 growth growth NOUN pk02c824z19 12 13 assays assay VERB pk02c824z19 12 14 to to PART pk02c824z19 12 15 observe observe VERB pk02c824z19 12 16 their their PRON pk02c824z19 12 17 ability ability NOUN pk02c824z19 12 18 to to PART pk02c824z19 12 19 tolerate tolerate VERB pk02c824z19 12 20 high high ADJ pk02c824z19 12 21 salt salt NOUN pk02c824z19 12 22 , , PUNCT pk02c824z19 12 23 antibiotics antibiotic NOUN pk02c824z19 12 24 , , PUNCT pk02c824z19 12 25 and and CCONJ pk02c824z19 12 26 neutrophil neutrophil NOUN pk02c824z19 12 27 killing killing NOUN pk02c824z19 12 28 and and CCONJ pk02c824z19 12 29 their their PRON pk02c824z19 12 30 ability ability NOUN pk02c824z19 12 31 to to PART pk02c824z19 12 32 form form VERB pk02c824z19 12 33 adherent adherent ADJ pk02c824z19 12 34 biofilms biofilm NOUN pk02c824z19 12 35 via via ADP pk02c824z19 12 36 crystal crystal PROPN pk02c824z19 12 37 violet violet PROPN pk02c824z19 12 38 staining staining NOUN pk02c824z19 12 39 . . PUNCT pk02c824z19 13 1 the the DET pk02c824z19 13 2 cells cell NOUN pk02c824z19 13 3 without without ADP pk02c824z19 13 4 srta srta PROPN pk02c824z19 13 5 were be AUX pk02c824z19 13 6 not not PART pk02c824z19 13 7 significantly significantly ADV pk02c824z19 13 8 different different ADJ pk02c824z19 13 9 in in ADP pk02c824z19 13 10 their their PRON pk02c824z19 13 11 ability ability NOUN pk02c824z19 13 12 to to PART pk02c824z19 13 13 grow grow VERB pk02c824z19 13 14 under under ADP pk02c824z19 13 15 favorable favorable ADJ pk02c824z19 13 16 conditions condition NOUN pk02c824z19 13 17 , , PUNCT pk02c824z19 13 18 but but CCONJ pk02c824z19 13 19 they they PRON pk02c824z19 13 20 were be AUX pk02c824z19 13 21 found find VERB pk02c824z19 13 22 to to PART pk02c824z19 13 23 have have AUX pk02c824z19 13 24 increased increase VERB pk02c824z19 13 25 susceptibilities susceptibility NOUN pk02c824z19 13 26 to to ADP pk02c824z19 13 27 high high ADJ pk02c824z19 13 28 salt salt NOUN pk02c824z19 13 29 and and CCONJ pk02c824z19 13 30 antibiotics antibiotic NOUN pk02c824z19 13 31 , , PUNCT pk02c824z19 13 32 indicating indicate VERB pk02c824z19 13 33 a a DET pk02c824z19 13 34 disruption disruption NOUN pk02c824z19 13 35 in in ADP pk02c824z19 13 36 their their PRON pk02c824z19 13 37 membrane membrane NOUN pk02c824z19 13 38 integrity integrity NOUN pk02c824z19 13 39 . . PUNCT pk02c824z19 14 1 the the DET pk02c824z19 14 2 δsrta δsrta PROPN pk02c824z19 14 3 mutant mutant NOUN pk02c824z19 14 4 was be AUX pk02c824z19 14 5 also also ADV pk02c824z19 14 6 found find VERB pk02c824z19 14 7 to to PART pk02c824z19 14 8 have have VERB pk02c824z19 14 9 a a DET pk02c824z19 14 10 reduced reduce VERB pk02c824z19 14 11 survivability survivability NOUN pk02c824z19 14 12 against against ADP pk02c824z19 14 13 direct direct ADJ pk02c824z19 14 14 neutrophil neutrophil NOUN pk02c824z19 14 15 exposure exposure NOUN pk02c824z19 14 16 and and CCONJ pk02c824z19 14 17 a a DET pk02c824z19 14 18 reduced reduce VERB pk02c824z19 14 19 ability ability NOUN pk02c824z19 14 20 to to PART pk02c824z19 14 21 form form VERB pk02c824z19 14 22 adherent adherent ADJ pk02c824z19 14 23 biofilms biofilm NOUN pk02c824z19 14 24 , , PUNCT pk02c824z19 14 25 indicating indicate VERB pk02c824z19 14 26 that that DET pk02c824z19 14 27 covalent covalent NOUN pk02c824z19 14 28 anchoring anchoring NOUN pk02c824z19 14 29 of of ADP pk02c824z19 14 30 lpxtg lpxtg PROPN pk02c824z19 14 31 - - PUNCT pk02c824z19 14 32 motif motif NOUN pk02c824z19 14 33 proteins protein NOUN pk02c824z19 14 34 is be AUX pk02c824z19 14 35 necessary necessary ADJ pk02c824z19 14 36 for for ADP pk02c824z19 14 37 full full ADJ pk02c824z19 14 38 functionality functionality NOUN pk02c824z19 14 39 in in ADP pk02c824z19 14 40 neutrophil neutrophil ADJ pk02c824z19 14 41 evasion evasion NOUN pk02c824z19 14 42 and and CCONJ pk02c824z19 14 43 cell cell NOUN pk02c824z19 14 44 adherence adherence PROPN pk02c824z19 14 45 . . PUNCT pk02c824z19 15 1 overall overall ADV pk02c824z19 15 2 , , PUNCT pk02c824z19 15 3 the the DET pk02c824z19 15 4 results result NOUN pk02c824z19 15 5 of of ADP pk02c824z19 15 6 this this DET pk02c824z19 15 7 study study NOUN pk02c824z19 15 8 suggest suggest VERB pk02c824z19 15 9 the the DET pk02c824z19 15 10 existence existence NOUN pk02c824z19 15 11 of of ADP pk02c824z19 15 12 an an DET pk02c824z19 15 13 alternative alternative ADJ pk02c824z19 15 14 mechanism mechanism NOUN pk02c824z19 15 15 for for ADP pk02c824z19 15 16 cell cell NOUN pk02c824z19 15 17 surface surface NOUN pk02c824z19 15 18 protein protein NOUN pk02c824z19 15 19 expression expression NOUN pk02c824z19 15 20 and and CCONJ pk02c824z19 15 21 the the DET pk02c824z19 15 22 necessity necessity NOUN pk02c824z19 15 23 for for ADP pk02c824z19 15 24 srta srta NOUN pk02c824z19 15 25 activity activity NOUN pk02c824z19 15 26 for for ADP pk02c824z19 15 27 bacterial bacterial ADJ pk02c824z19 15 28 viability viability NOUN pk02c824z19 15 29 and and CCONJ pk02c824z19 15 30 hardiness hardiness NOUN pk02c824z19 15 31 . . PUNCT