id sid tid token lemma pos gm80ht2734t 1 1 one one NUM gm80ht2734t 1 2 of of ADP gm80ht2734t 1 3 the the DET gm80ht2734t 1 4 key key ADJ gm80ht2734t 1 5 proteins protein NOUN gm80ht2734t 1 6 involve involve VERB gm80ht2734t 1 7 in in ADP gm80ht2734t 1 8 clot clot NOUN gm80ht2734t 1 9 lysis lysis NOUN gm80ht2734t 1 10 in in ADP gm80ht2734t 1 11 vertebrate vertebrate ADJ gm80ht2734t 1 12 organisms organism NOUN gm80ht2734t 1 13 is be AUX gm80ht2734t 1 14 plasminogen plasminogen NOUN gm80ht2734t 1 15 ( ( PUNCT gm80ht2734t 1 16 glu1 glu1 PROPN gm80ht2734t 1 17 - - PUNCT gm80ht2734t 1 18 pg pg PROPN gm80ht2734t 1 19 ) ) PUNCT gm80ht2734t 1 20 . . PUNCT gm80ht2734t 2 1 it it PRON gm80ht2734t 2 2 is be AUX gm80ht2734t 2 3 the the DET gm80ht2734t 2 4 circulating circulate VERB gm80ht2734t 2 5 zymogen zymogen NOUN gm80ht2734t 2 6 of of ADP gm80ht2734t 2 7 the the DET gm80ht2734t 2 8 serine serine NOUN gm80ht2734t 2 9 protease protease NOUN gm80ht2734t 2 10 plasmin plasmin NOUN gm80ht2734t 2 11 ( ( PUNCT gm80ht2734t 2 12 pm pm PROPN gm80ht2734t 2 13 ) ) PUNCT gm80ht2734t 2 14 . . PUNCT gm80ht2734t 3 1 generation generation NOUN gm80ht2734t 3 2 of of ADP gm80ht2734t 3 3 the the DET gm80ht2734t 3 4 active active ADJ gm80ht2734t 3 5 enzyme enzyme NOUN gm80ht2734t 3 6 requires require VERB gm80ht2734t 3 7 cleavage cleavage NOUN gm80ht2734t 3 8 of of ADP gm80ht2734t 3 9 the the DET gm80ht2734t 3 10 arg561 arg561 NOUN gm80ht2734t 3 11 - - PUNCT gm80ht2734t 3 12 val562 val562 ADJ gm80ht2734t 3 13 bond bond NOUN gm80ht2734t 3 14 and and CCONJ gm80ht2734t 3 15 proteolytic proteolytic ADJ gm80ht2734t 3 16 removal removal NOUN gm80ht2734t 3 17 of of ADP gm80ht2734t 3 18 the the DET gm80ht2734t 3 19 77 77 NUM gm80ht2734t 3 20 - - PUNCT gm80ht2734t 3 21 residue residue NOUN gm80ht2734t 3 22 , , PUNCT gm80ht2734t 3 23 n n ADJ gm80ht2734t 3 24 - - PUNCT gm80ht2734t 3 25 terminal terminal ADJ gm80ht2734t 3 26 activation activation NOUN gm80ht2734t 3 27 peptide peptide NOUN gm80ht2734t 3 28 ( ( PUNCT gm80ht2734t 3 29 ap ap PROPN gm80ht2734t 3 30 ) ) PUNCT gm80ht2734t 3 31 . . PUNCT gm80ht2734t 4 1 binding bind VERB gm80ht2734t 4 2 of of ADP gm80ht2734t 4 3 lys lys X gm80ht2734t 4 4 and and CCONJ gm80ht2734t 4 5 lys lys ADJ gm80ht2734t 4 6 - - PUNCT gm80ht2734t 4 7 analogs analogs ADJ gm80ht2734t 4 8 by by ADP gm80ht2734t 4 9 pg pg PROPN gm80ht2734t 4 10 / / SYM gm80ht2734t 4 11 pm pm NOUN gm80ht2734t 4 12 is be AUX gm80ht2734t 4 13 mediated mediate VERB gm80ht2734t 4 14 by by ADP gm80ht2734t 4 15 kringle kringle NOUN gm80ht2734t 4 16 domains domain NOUN gm80ht2734t 4 17 ( ( PUNCT gm80ht2734t 4 18 k k PROPN gm80ht2734t 4 19 ) ) PUNCT gm80ht2734t 4 20 , , PUNCT gm80ht2734t 4 21 and and CCONJ gm80ht2734t 4 22 they they PRON gm80ht2734t 4 23 determine determine VERB gm80ht2734t 4 24 the the DET gm80ht2734t 4 25 interaction interaction NOUN gm80ht2734t 4 26 of of ADP gm80ht2734t 4 27 pg pg PROPN gm80ht2734t 4 28 / / SYM gm80ht2734t 4 29 pm pm NOUN gm80ht2734t 4 30 with with ADP gm80ht2734t 4 31 substrates substrate NOUN gm80ht2734t 4 32 , , PUNCT gm80ht2734t 4 33 receptors receptor NOUN gm80ht2734t 4 34 , , PUNCT gm80ht2734t 4 35 activators activator NOUN gm80ht2734t 4 36 and and CCONJ gm80ht2734t 4 37 inhibitors inhibitor NOUN gm80ht2734t 4 38 . . PUNCT gm80ht2734t 5 1 activation activation NOUN gm80ht2734t 5 2 rate rate NOUN gm80ht2734t 5 3 of of ADP gm80ht2734t 5 4 pg pg PROPN gm80ht2734t 5 5 is be AUX gm80ht2734t 5 6 highly highly ADV gm80ht2734t 5 7 dependent dependent ADJ gm80ht2734t 5 8 on on ADP gm80ht2734t 5 9 its its PRON gm80ht2734t 5 10 conformation conformation NOUN gm80ht2734t 5 11 and and CCONJ gm80ht2734t 5 12 it it PRON gm80ht2734t 5 13 has have AUX gm80ht2734t 5 14 been be AUX gm80ht2734t 5 15 shown show VERB gm80ht2734t 5 16 that that SCONJ gm80ht2734t 5 17 the the DET gm80ht2734t 5 18 zymogen zymogen NOUN gm80ht2734t 5 19 can can AUX gm80ht2734t 5 20 assume assume VERB gm80ht2734t 5 21 at at ADP gm80ht2734t 5 22 least least ADV gm80ht2734t 5 23 two two NUM gm80ht2734t 5 24 major major ADJ gm80ht2734t 5 25 conformational conformational ADJ gm80ht2734t 5 26 states state NOUN gm80ht2734t 5 27 ; ; PUNCT gm80ht2734t 5 28 closed closed ADJ gm80ht2734t 5 29 and and CCONJ gm80ht2734t 5 30 open open ADJ gm80ht2734t 5 31 . . PUNCT gm80ht2734t 6 1 the the DET gm80ht2734t 6 2 former former ADJ gm80ht2734t 6 3 is be AUX gm80ht2734t 6 4 resistant resistant ADJ gm80ht2734t 6 5 to to PART gm80ht2734t 6 6 activation activation VERB gm80ht2734t 6 7 and and CCONJ gm80ht2734t 6 8 is be AUX gm80ht2734t 6 9 detected detect VERB gm80ht2734t 6 10 in in ADP gm80ht2734t 6 11 the the DET gm80ht2734t 6 12 presence presence NOUN gm80ht2734t 6 13 of of ADP gm80ht2734t 6 14 cl- cl- SPACE gm80ht2734t 6 15 , , PUNCT gm80ht2734t 6 16 and and CCONJ gm80ht2734t 6 17 the the DET gm80ht2734t 6 18 latter latter ADJ gm80ht2734t 6 19 is be AUX gm80ht2734t 6 20 more more ADV gm80ht2734t 6 21 easily easily ADV gm80ht2734t 6 22 activated activate VERB gm80ht2734t 6 23 and and CCONJ gm80ht2734t 6 24 is be AUX gm80ht2734t 6 25 observed observe VERB gm80ht2734t 6 26 in in ADP gm80ht2734t 6 27 the the DET gm80ht2734t 6 28 presence presence NOUN gm80ht2734t 6 29 of of ADP gm80ht2734t 6 30 lys lys PROPN gm80ht2734t 6 31 and and CCONJ gm80ht2734t 6 32 lys lys X gm80ht2734t 6 33 analogs analog NOUN gm80ht2734t 6 34 , , PUNCT gm80ht2734t 6 35 such such ADJ gm80ht2734t 6 36 as as ADP gm80ht2734t 6 37 6 6 NUM gm80ht2734t 6 38 - - PUNCT gm80ht2734t 6 39 amino amino NOUN gm80ht2734t 6 40 hexanoic hexanoic ADJ gm80ht2734t 6 41 acid acid NOUN gm80ht2734t 6 42 ( ( PUNCT gm80ht2734t 6 43 6aha 6aha PROPN gm80ht2734t 6 44 ) ) PUNCT gm80ht2734t 6 45 . . PUNCT gm80ht2734t 7 1 pg pg PROPN gm80ht2734t 7 2 lacking lack VERB gm80ht2734t 7 3 the the DET gm80ht2734t 7 4 ap ap PROPN gm80ht2734t 7 5 ( ( PUNCT gm80ht2734t 7 6 lys78 lys78 PROPN gm80ht2734t 7 7 - - PUNCT gm80ht2734t 7 8 pg pg NOUN gm80ht2734t 7 9 ) ) PUNCT gm80ht2734t 7 10 exists exist VERB gm80ht2734t 7 11 in in ADP gm80ht2734t 7 12 the the DET gm80ht2734t 7 13 open open ADJ gm80ht2734t 7 14 conformation conformation NOUN gm80ht2734t 7 15 , , PUNCT gm80ht2734t 7 16 suggesting suggest VERB gm80ht2734t 7 17 that that SCONJ gm80ht2734t 7 18 intramolecular intramolecular ADJ gm80ht2734t 7 19 interactions interaction NOUN gm80ht2734t 7 20 between between ADP gm80ht2734t 7 21 the the DET gm80ht2734t 7 22 ap ap PROPN gm80ht2734t 7 23 and and CCONJ gm80ht2734t 7 24 the the DET gm80ht2734t 7 25 kringle kringle NOUN gm80ht2734t 7 26 domains domain NOUN gm80ht2734t 7 27 of of ADP gm80ht2734t 7 28 glu1 glu1 PROPN gm80ht2734t 7 29 - - PUNCT gm80ht2734t 7 30 pg pg PROPN gm80ht2734t 7 31 induce induce VERB gm80ht2734t 7 32 the the DET gm80ht2734t 7 33 closed closed ADJ gm80ht2734t 7 34 form form NOUN gm80ht2734t 7 35 of of ADP gm80ht2734t 7 36 the the DET gm80ht2734t 7 37 zymogen zymogen NOUN gm80ht2734t 7 38 . . NOUN gm80ht2734t 7 39 to to PART gm80ht2734t 7 40 assess assess VERB gm80ht2734t 7 41 the the DET gm80ht2734t 7 42 contributions contribution NOUN gm80ht2734t 7 43 of of ADP gm80ht2734t 7 44 specific specific ADJ gm80ht2734t 7 45 domains domain NOUN gm80ht2734t 7 46 on on ADP gm80ht2734t 7 47 the the DET gm80ht2734t 7 48 maintenance maintenance NOUN gm80ht2734t 7 49 of of ADP gm80ht2734t 7 50 the the DET gm80ht2734t 7 51 closed closed ADJ gm80ht2734t 7 52 form form NOUN gm80ht2734t 7 53 , , PUNCT gm80ht2734t 7 54 we we PRON gm80ht2734t 7 55 have have AUX gm80ht2734t 7 56 generated generate VERB gm80ht2734t 7 57 a a DET gm80ht2734t 7 58 panel panel NOUN gm80ht2734t 7 59 of of ADP gm80ht2734t 7 60 recombinant recombinant ADJ gm80ht2734t 7 61 pg pg PROPN gm80ht2734t 7 62 truncation truncation NOUN gm80ht2734t 7 63 mutants mutant NOUN gm80ht2734t 7 64 , , PUNCT gm80ht2734t 7 65 which which PRON gm80ht2734t 7 66 include include VERB gm80ht2734t 7 67 apk1 apk1 PROPN gm80ht2734t 7 68 - - PUNCT gm80ht2734t 7 69 3 3 NUM gm80ht2734t 7 70 ( ( PUNCT gm80ht2734t 7 71 residues residue VERB gm80ht2734t 7 72 1 1 NUM gm80ht2734t 7 73 - - SYM gm80ht2734t 7 74 338 338 NUM gm80ht2734t 7 75 of of ADP gm80ht2734t 7 76 pg pg PROPN gm80ht2734t 7 77 ) ) PUNCT gm80ht2734t 7 78 , , PUNCT gm80ht2734t 7 79 apk1 apk1 PROPN gm80ht2734t 7 80 - - PUNCT gm80ht2734t 7 81 4 4 NUM gm80ht2734t 7 82 ( ( PUNCT gm80ht2734t 7 83 residues residue VERB gm80ht2734t 7 84 1 1 NUM gm80ht2734t 7 85 - - SYM gm80ht2734t 7 86 438 438 NUM gm80ht2734t 7 87 of of ADP gm80ht2734t 7 88 pg pg PROPN gm80ht2734t 7 89 ) ) PUNCT gm80ht2734t 7 90 and and CCONJ gm80ht2734t 7 91 apk1 apk1 PROPN gm80ht2734t 7 92 - - PUNCT gm80ht2734t 7 93 5 5 NUM gm80ht2734t 7 94 ( ( PUNCT gm80ht2734t 7 95 residues residue VERB gm80ht2734t 7 96 1 1 NUM gm80ht2734t 7 97 - - SYM gm80ht2734t 7 98 543 543 NUM gm80ht2734t 7 99 of of ADP gm80ht2734t 7 100 pg pg PROPN gm80ht2734t 7 101 ) ) PUNCT gm80ht2734t 7 102 . . PUNCT gm80ht2734t 8 1 variants variant NOUN gm80ht2734t 8 2 of of ADP gm80ht2734t 8 3 apk1 apk1 PROPN gm80ht2734t 8 4 - - SYM gm80ht2734t 8 5 5 5 NUM gm80ht2734t 8 6 carrying carry VERB gm80ht2734t 8 7 single single ADJ gm80ht2734t 8 8 point point NOUN gm80ht2734t 8 9 mutations mutation NOUN gm80ht2734t 8 10 in in ADP gm80ht2734t 8 11 every every DET gm80ht2734t 8 12 lys lys ADJ gm80ht2734t 8 13 residue residue NOUN gm80ht2734t 8 14 of of ADP gm80ht2734t 8 15 the the DET gm80ht2734t 8 16 ap ap PROPN gm80ht2734t 8 17 have have AUX gm80ht2734t 8 18 also also ADV gm80ht2734t 8 19 been be AUX gm80ht2734t 8 20 generated generate VERB gm80ht2734t 8 21 . . PUNCT gm80ht2734t 9 1 analytical analytical ADJ gm80ht2734t 9 2 ultracentrifugation ultracentrifugation NOUN gm80ht2734t 9 3 , , PUNCT gm80ht2734t 9 4 differential differential ADJ gm80ht2734t 9 5 scanning scan VERB gm80ht2734t 9 6 calorimetry calorimetry NOUN gm80ht2734t 9 7 and and CCONJ gm80ht2734t 9 8 circular circular ADJ gm80ht2734t 9 9 dichroism dichroism NOUN gm80ht2734t 9 10 , , PUNCT gm80ht2734t 9 11 have have AUX gm80ht2734t 9 12 been be AUX gm80ht2734t 9 13 used use VERB gm80ht2734t 9 14 to to PART gm80ht2734t 9 15 evaluate evaluate VERB gm80ht2734t 9 16 the the DET gm80ht2734t 9 17 hydrodynamic hydrodynamic ADJ gm80ht2734t 9 18 properties property NOUN gm80ht2734t 9 19 , , PUNCT gm80ht2734t 9 20 conformational conformational ADJ gm80ht2734t 9 21 stability stability NOUN gm80ht2734t 9 22 and and CCONJ gm80ht2734t 9 23 secondary secondary ADJ gm80ht2734t 9 24 structure structure NOUN gm80ht2734t 9 25 alterations alteration NOUN gm80ht2734t 9 26 of of ADP gm80ht2734t 9 27 the the DET gm80ht2734t 9 28 generated generate VERB gm80ht2734t 9 29 constructs construct NOUN gm80ht2734t 9 30 in in ADP gm80ht2734t 9 31 the the DET gm80ht2734t 9 32 presence presence NOUN gm80ht2734t 9 33 of of ADP gm80ht2734t 9 34 clor clor NOUN gm80ht2734t 9 35 6aha 6aha PROPN gm80ht2734t 9 36 . . PUNCT gm80ht2734t 10 1 our our PRON gm80ht2734t 10 2 results result NOUN gm80ht2734t 10 3 suggest suggest VERB gm80ht2734t 10 4 a a DET gm80ht2734t 10 5 critical critical ADJ gm80ht2734t 10 6 , , PUNCT gm80ht2734t 10 7 but but CCONJ gm80ht2734t 10 8 not not PART gm80ht2734t 10 9 exclusive exclusive ADJ gm80ht2734t 10 10 , , PUNCT gm80ht2734t 10 11 role role NOUN gm80ht2734t 10 12 for for ADP gm80ht2734t 10 13 kringle kringle NOUN gm80ht2734t 10 14 5 5 NUM gm80ht2734t 10 15 and and CCONJ gm80ht2734t 10 16 lys lys ADJ gm80ht2734t 10 17 residues residue VERB gm80ht2734t 10 18 50 50 NUM gm80ht2734t 10 19 and and CCONJ gm80ht2734t 10 20 62 62 NUM gm80ht2734t 10 21 of of ADP gm80ht2734t 10 22 the the DET gm80ht2734t 10 23 ap ap PROPN gm80ht2734t 10 24 in in ADP gm80ht2734t 10 25 the the DET gm80ht2734t 10 26 stabilization stabilization NOUN gm80ht2734t 10 27 of of ADP gm80ht2734t 10 28 the the DET gm80ht2734t 10 29 closed closed ADJ gm80ht2734t 10 30 conformation conformation NOUN gm80ht2734t 10 31 of of ADP gm80ht2734t 10 32 glu1 glu1 PROPN gm80ht2734t 10 33 - - PUNCT gm80ht2734t 10 34 pg pg PROPN gm80ht2734t 10 35 . . PUNCT gm80ht2734t 11 1 lys lys PROPN gm80ht2734t 11 2 to to ADP gm80ht2734t 11 3 ala ala PROPN gm80ht2734t 11 4 mutations mutation NOUN gm80ht2734t 11 5 in in ADP gm80ht2734t 11 6 residues residue NOUN gm80ht2734t 11 7 50 50 NUM gm80ht2734t 11 8 and and CCONJ gm80ht2734t 11 9 62 62 NUM gm80ht2734t 11 10 of of ADP gm80ht2734t 11 11 the the DET gm80ht2734t 11 12 ap ap PROPN gm80ht2734t 11 13 appear appear VERB gm80ht2734t 11 14 to to PART gm80ht2734t 11 15 have have VERB gm80ht2734t 11 16 destabilizing destabilizing ADJ gm80ht2734t 11 17 effects effect NOUN gm80ht2734t 11 18 in in ADP gm80ht2734t 11 19 the the DET gm80ht2734t 11 20 apk1 apk1 PROPN gm80ht2734t 11 21 - - PUNCT gm80ht2734t 11 22 5 5 NUM gm80ht2734t 11 23 construct construct NOUN gm80ht2734t 11 24 . . PUNCT gm80ht2734t 12 1 direct direct ADJ gm80ht2734t 12 2 interaction interaction NOUN gm80ht2734t 12 3 of of ADP gm80ht2734t 12 4 ap ap PROPN gm80ht2734t 12 5 and and CCONJ gm80ht2734t 12 6 isolated isolated ADJ gm80ht2734t 12 7 kringles kringle NOUN gm80ht2734t 12 8 was be AUX gm80ht2734t 12 9 demonstrated demonstrate VERB gm80ht2734t 12 10 and and CCONJ gm80ht2734t 12 11 the the DET gm80ht2734t 12 12 binding binding NOUN gm80ht2734t 12 13 of of ADP gm80ht2734t 12 14 the the DET gm80ht2734t 12 15 ap ap PROPN gm80ht2734t 12 16 to to ADP gm80ht2734t 12 17 glu1 glu1 PROPN gm80ht2734t 12 18 - - PUNCT gm80ht2734t 12 19 pg pg PROPN gm80ht2734t 12 20 induces induce VERB gm80ht2734t 12 21 an an DET gm80ht2734t 12 22 enhancement enhancement NOUN gm80ht2734t 12 23 in in ADP gm80ht2734t 12 24 the the DET gm80ht2734t 12 25 activation activation NOUN gm80ht2734t 12 26 rate rate NOUN gm80ht2734t 12 27 of of ADP gm80ht2734t 12 28 the the DET gm80ht2734t 12 29 zymogen zymogen NOUN gm80ht2734t 12 30 . . PUNCT gm80ht2734t 13 1 moreover moreover ADV gm80ht2734t 13 2 , , PUNCT gm80ht2734t 13 3 the the DET gm80ht2734t 13 4 interactions interaction NOUN gm80ht2734t 13 5 of of ADP gm80ht2734t 13 6 pg pg PROPN gm80ht2734t 13 7 with with ADP gm80ht2734t 13 8 pam pam PROPN gm80ht2734t 13 9 ( ( PUNCT gm80ht2734t 13 10 pg pg PROPN gm80ht2734t 13 11 - - PUNCT gm80ht2734t 13 12 binding bind VERB gm80ht2734t 13 13 group group NOUN gm80ht2734t 13 14 a a DET gm80ht2734t 13 15 streptococcal streptococcal ADJ gm80ht2734t 13 16 m m ADJ gm80ht2734t 13 17 - - PUNCT gm80ht2734t 13 18 like like ADJ gm80ht2734t 13 19 protein protein NOUN gm80ht2734t 13 20 ) ) PUNCT gm80ht2734t 13 21 and and CCONJ gm80ht2734t 13 22 its its PRON gm80ht2734t 13 23 pg pg PROPN gm80ht2734t 13 24 binding bind VERB gm80ht2734t 13 25 motif motif NOUN gm80ht2734t 13 26 ( ( PUNCT gm80ht2734t 13 27 vek30 vek30 NOUN gm80ht2734t 13 28 ) ) PUNCT gm80ht2734t 13 29 were be AUX gm80ht2734t 13 30 characterized characterize VERB gm80ht2734t 13 31 . . PUNCT gm80ht2734t 14 1 here here ADV gm80ht2734t 14 2 , , PUNCT gm80ht2734t 14 3 we we PRON gm80ht2734t 14 4 demonstrate demonstrate VERB gm80ht2734t 14 5 the the DET gm80ht2734t 14 6 existence existence NOUN gm80ht2734t 14 7 of of ADP gm80ht2734t 14 8 a a DET gm80ht2734t 14 9 partly partly ADV gm80ht2734t 14 10 - - PUNCT gm80ht2734t 14 11 open open ADJ gm80ht2734t 14 12 conformational conformational ADJ gm80ht2734t 14 13 state state NOUN gm80ht2734t 14 14 of of ADP gm80ht2734t 14 15 pg pg PROPN gm80ht2734t 14 16 when when SCONJ gm80ht2734t 14 17 bound bind VERB gm80ht2734t 14 18 to to PART gm80ht2734t 14 19 pam pam PROPN gm80ht2734t 14 20 or or CCONJ gm80ht2734t 14 21 vek30 vek30 PROPN gm80ht2734t 14 22 . . PUNCT gm80ht2734t 15 1 this this DET gm80ht2734t 15 2 novel novel ADJ gm80ht2734t 15 3 state state NOUN gm80ht2734t 15 4 of of ADP gm80ht2734t 15 5 pg pg PROPN gm80ht2734t 15 6 can can AUX gm80ht2734t 15 7 be be AUX gm80ht2734t 15 8 more more ADV gm80ht2734t 15 9 effectively effectively ADV gm80ht2734t 15 10 activated activate VERB gm80ht2734t 15 11 by by ADP gm80ht2734t 15 12 pg pg PROPN gm80ht2734t 15 13 activator activator NOUN gm80ht2734t 15 14 such such ADJ gm80ht2734t 15 15 as as ADP gm80ht2734t 15 16 urokinase urokinase NOUN gm80ht2734t 15 17 and and CCONJ gm80ht2734t 15 18 streptokinase streptokinase PROPN gm80ht2734t 15 19 . . PUNCT