id sid tid token lemma pos gb19f477842 1 1 gephyronic gephyronic PROPN gb19f477842 1 2 acid acid PROPN gb19f477842 1 3 is be AUX gb19f477842 1 4 a a DET gb19f477842 1 5 secondary secondary ADJ gb19f477842 1 6 metabolite metabolite NOUN gb19f477842 1 7 exhibiting exhibit VERB gb19f477842 1 8 potent potent ADJ gb19f477842 1 9 anti anti ADJ gb19f477842 1 10 - - ADJ gb19f477842 1 11 cancer cancer ADJ gb19f477842 1 12 and and CCONJ gb19f477842 1 13 antibiotic antibiotic ADJ gb19f477842 1 14 activities activity NOUN gb19f477842 1 15 . . PUNCT gb19f477842 2 1 this this DET gb19f477842 2 2 polyketide polyketide NOUN gb19f477842 2 3 natural natural ADJ gb19f477842 2 4 product product NOUN gb19f477842 2 5 was be AUX gb19f477842 2 6 first first ADV gb19f477842 2 7 isolated isolate VERB gb19f477842 2 8 at at ADP gb19f477842 2 9 the the DET gb19f477842 2 10 hzi hzi PROPN gb19f477842 2 11 ( ( PUNCT gb19f477842 2 12 helmholtz helmholtz NOUN gb19f477842 2 13 - - PUNCT gb19f477842 2 14 zentrum zentrum VERB gb19f477842 2 15 fì_r fì_r NOUN gb19f477842 2 16 infektionsforschung infektionsforschung NOUN gb19f477842 2 17 ) ) PUNCT gb19f477842 2 18 by by ADP gb19f477842 2 19 sasse sasse PROPN gb19f477842 2 20 , , PUNCT gb19f477842 2 21 h h PROPN gb19f477842 2 22 fle fle PROPN gb19f477842 2 23 , , PUNCT gb19f477842 2 24 and and CCONJ gb19f477842 2 25 reichenbach reichenbach NOUN gb19f477842 2 26 from from ADP gb19f477842 2 27 the the DET gb19f477842 2 28 myxobacterium myxobacterium ADJ gb19f477842 2 29 archangium archangium NOUN gb19f477842 2 30 gephyra gephyra PROPN gb19f477842 2 31 in in ADP gb19f477842 2 32 1995 1995 NUM gb19f477842 2 33 . . PUNCT gb19f477842 3 1 it it PRON gb19f477842 3 2 was be AUX gb19f477842 3 3 reported report VERB gb19f477842 3 4 to to PART gb19f477842 3 5 exist exist VERB gb19f477842 3 6 as as ADP gb19f477842 3 7 a a DET gb19f477842 3 8 separable separable ADJ gb19f477842 3 9 mixture mixture NOUN gb19f477842 3 10 of of ADP gb19f477842 3 11 open open ADJ gb19f477842 3 12 keto keto NOUN gb19f477842 3 13 - - PUNCT gb19f477842 3 14 alcohol alcohol NOUN gb19f477842 3 15 and and CCONJ gb19f477842 3 16 closed close VERB gb19f477842 3 17 hemiketal hemiketal ADJ gb19f477842 3 18 forms form NOUN gb19f477842 3 19 , , PUNCT gb19f477842 3 20 but but CCONJ gb19f477842 3 21 only only ADV gb19f477842 3 22 the the DET gb19f477842 3 23 gross gross ADJ gb19f477842 3 24 structure structure NOUN gb19f477842 3 25 was be AUX gb19f477842 3 26 identified identify VERB gb19f477842 3 27 . . PUNCT gb19f477842 4 1 gephyronic gephyronic PROPN gb19f477842 4 2 acid acid PROPN gb19f477842 4 3 has have AUX gb19f477842 4 4 been be AUX gb19f477842 4 5 shown show VERB gb19f477842 4 6 to to PART gb19f477842 4 7 inhibit inhibit VERB gb19f477842 4 8 the the DET gb19f477842 4 9 growth growth NOUN gb19f477842 4 10 of of ADP gb19f477842 4 11 yeast yeast NOUN gb19f477842 4 12 and and CCONJ gb19f477842 4 13 mold mold NOUN gb19f477842 4 14 in in ADP gb19f477842 4 15 the the DET gb19f477842 4 16 micromolar micromolar ADJ gb19f477842 4 17 range range NOUN gb19f477842 4 18 ( ( PUNCT gb19f477842 4 19 mic mic NOUN gb19f477842 4 20 1 1 NUM gb19f477842 4 21 - - SYM gb19f477842 4 22 25 25 NUM gb19f477842 4 23 & & CCONJ gb19f477842 4 24 # # SYM gb19f477842 4 25 956;g 956;g PROPN gb19f477842 4 26 / / SYM gb19f477842 4 27 ml ml PROPN gb19f477842 4 28 ) ) PUNCT gb19f477842 4 29 ; ; PUNCT gb19f477842 4 30 it it PRON gb19f477842 4 31 also also ADV gb19f477842 4 32 exhibits exhibit VERB gb19f477842 4 33 a a DET gb19f477842 4 34 cytostatic cytostatic ADJ gb19f477842 4 35 effect effect NOUN gb19f477842 4 36 on on ADP gb19f477842 4 37 mammalian mammalian ADJ gb19f477842 4 38 cell cell NOUN gb19f477842 4 39 cultures culture NOUN gb19f477842 4 40 ( ( PUNCT gb19f477842 4 41 human human ADJ gb19f477842 4 42 cervix cervix NOUN gb19f477842 4 43 , , PUNCT gb19f477842 4 44 human human ADJ gb19f477842 4 45 leukemia leukemia NOUN gb19f477842 4 46 , , PUNCT gb19f477842 4 47 human human ADJ gb19f477842 4 48 breast breast NOUN gb19f477842 4 49 ) ) PUNCT gb19f477842 4 50 in in ADP gb19f477842 4 51 the the DET gb19f477842 4 52 nanomolar nanomolar ADJ gb19f477842 4 53 range range NOUN gb19f477842 4 54 ( ( PUNCT gb19f477842 4 55 ic50 ic50 PROPN gb19f477842 4 56 10 10 NUM gb19f477842 4 57 - - SYM gb19f477842 4 58 60 60 NUM gb19f477842 4 59 ng ng PROPN gb19f477842 4 60 / / SYM gb19f477842 4 61 ml ml PROPN gb19f477842 4 62 ) ) PUNCT gb19f477842 4 63 . . PUNCT gb19f477842 5 1 an an DET gb19f477842 5 2 in in ADP gb19f477842 5 3 vitro vitro X gb19f477842 5 4 translation translation NOUN gb19f477842 5 5 assay assay NOUN gb19f477842 5 6 demonstrated demonstrate VERB gb19f477842 5 7 that that SCONJ gb19f477842 5 8 gephyronic gephyronic PROPN gb19f477842 5 9 acid acid NOUN gb19f477842 5 10 is be AUX gb19f477842 5 11 a a DET gb19f477842 5 12 specific specific ADJ gb19f477842 5 13 inhibitor inhibitor NOUN gb19f477842 5 14 of of ADP gb19f477842 5 15 eukaryotic eukaryotic ADJ gb19f477842 5 16 protein protein NOUN gb19f477842 5 17 synthesis synthesis NOUN gb19f477842 5 18 ( ( PUNCT gb19f477842 5 19 ic50 ic50 PROPN gb19f477842 5 20 66 66 NUM gb19f477842 5 21 - - SYM gb19f477842 5 22 80 80 NUM gb19f477842 5 23 ng ng PROPN gb19f477842 5 24 / / SYM gb19f477842 5 25 ml ml PROPN gb19f477842 5 26 ) ) PUNCT gb19f477842 5 27 . . PUNCT gb19f477842 6 1 our our PRON gb19f477842 6 2 recent recent ADJ gb19f477842 6 3 total total ADJ gb19f477842 6 4 synthesis synthesis NOUN gb19f477842 6 5 and and CCONJ gb19f477842 6 6 structural structural ADJ gb19f477842 6 7 reassignment reassignment NOUN gb19f477842 6 8 of of ADP gb19f477842 6 9 gephyronic gephyronic PROPN gb19f477842 6 10 acid acid PROPN gb19f477842 6 11 incorporates incorporate VERB gb19f477842 6 12 a a DET gb19f477842 6 13 synthetic synthetic ADJ gb19f477842 6 14 route route NOUN gb19f477842 6 15 that that PRON gb19f477842 6 16 provides provide VERB gb19f477842 6 17 a a DET gb19f477842 6 18 means means NOUN gb19f477842 6 19 by by ADP gb19f477842 6 20 which which PRON gb19f477842 6 21 to to PART gb19f477842 6 22 prepare prepare VERB gb19f477842 6 23 gephyronic gephyronic PROPN gb19f477842 6 24 acid acid NOUN gb19f477842 6 25 efficiently efficiently ADV gb19f477842 6 26 and and CCONJ gb19f477842 6 27 selectively selectively ADV gb19f477842 6 28 and and CCONJ gb19f477842 6 29 has have AUX gb19f477842 6 30 provided provide VERB gb19f477842 6 31 significant significant ADJ gb19f477842 6 32 quantities quantity NOUN gb19f477842 6 33 , , PUNCT gb19f477842 6 34 thus thus ADV gb19f477842 6 35 facilitating facilitate VERB gb19f477842 6 36 further further ADJ gb19f477842 6 37 biological biological ADJ gb19f477842 6 38 evaluation evaluation NOUN gb19f477842 6 39 towards towards ADP gb19f477842 6 40 identifying identify VERB gb19f477842 6 41 gephyronic gephyronic PROPN gb19f477842 6 42 acids acid NOUN gb19f477842 6 43 specific specific ADJ gb19f477842 6 44 mode mode NOUN gb19f477842 6 45 of of ADP gb19f477842 6 46 action action NOUN gb19f477842 6 47 in in ADP gb19f477842 6 48 cancer cancer NOUN gb19f477842 6 49 cells cell NOUN gb19f477842 6 50 . . PUNCT gb19f477842 7 1 the the DET gb19f477842 7 2 newly newly ADV gb19f477842 7 3 proposed propose VERB gb19f477842 7 4 structure structure NOUN gb19f477842 7 5 for for ADP gb19f477842 7 6 gephyronic gephyronic PROPN gb19f477842 7 7 acid acid PROPN gb19f477842 7 8 also also ADV gb19f477842 7 9 provides provide VERB gb19f477842 7 10 further further ADJ gb19f477842 7 11 insight insight NOUN gb19f477842 7 12 in in ADP gb19f477842 7 13 to to ADP gb19f477842 7 14 the the DET gb19f477842 7 15 structural structural ADJ gb19f477842 7 16 relationship relationship NOUN gb19f477842 7 17 between between ADP gb19f477842 7 18 related relate VERB gb19f477842 7 19 eukaryotic eukaryotic ADJ gb19f477842 7 20 protein protein NOUN gb19f477842 7 21 synthesis synthesis NOUN gb19f477842 7 22 inhibitors inhibitor NOUN gb19f477842 7 23 tedanolide tedanolide ADJ gb19f477842 7 24 , , PUNCT gb19f477842 7 25 myriaporone myriaporone NOUN gb19f477842 7 26 , , PUNCT gb19f477842 7 27 and and CCONJ gb19f477842 7 28 pederin pederin PROPN gb19f477842 7 29 . . PUNCT gb19f477842 8 1 thus thus ADV gb19f477842 8 2 , , PUNCT gb19f477842 8 3 gephyronic gephyronic PROPN gb19f477842 8 4 acid acid PROPN gb19f477842 8 5 may may AUX gb19f477842 8 6 represent represent VERB gb19f477842 8 7 a a DET gb19f477842 8 8 potential potential ADJ gb19f477842 8 9 pharmacophoric pharmacophoric ADJ gb19f477842 8 10 link link NOUN gb19f477842 8 11 between between ADP gb19f477842 8 12 structurally structurally ADV gb19f477842 8 13 distinct distinct ADJ gb19f477842 8 14 classes class NOUN gb19f477842 8 15 of of ADP gb19f477842 8 16 biologically biologically ADV gb19f477842 8 17 active active ADJ gb19f477842 8 18 polyketides polyketide NOUN gb19f477842 8 19 with with ADP gb19f477842 8 20 related related ADJ gb19f477842 8 21 modes mode NOUN gb19f477842 8 22 of of ADP gb19f477842 8 23 action action NOUN gb19f477842 8 24 . . PUNCT gb19f477842 9 1 moreover moreover ADV gb19f477842 9 2 , , PUNCT gb19f477842 9 3 we we PRON gb19f477842 9 4 sought seek VERB gb19f477842 9 5 to to PART gb19f477842 9 6 identify identify VERB gb19f477842 9 7 and and CCONJ gb19f477842 9 8 characterize characterize VERB gb19f477842 9 9 the the DET gb19f477842 9 10 polyketide polyketide NOUN gb19f477842 9 11 synthase synthase NOUN gb19f477842 9 12 ( ( PUNCT gb19f477842 9 13 pks pks NOUN gb19f477842 9 14 ) ) PUNCT gb19f477842 9 15 gene gene NOUN gb19f477842 9 16 cluster cluster NOUN gb19f477842 9 17 responsible responsible ADJ gb19f477842 9 18 for for ADP gb19f477842 9 19 gephyronic gephyronic PROPN gb19f477842 9 20 acid acid NOUN gb19f477842 9 21 production production NOUN gb19f477842 9 22 in in ADP gb19f477842 9 23 the the DET gb19f477842 9 24 producing produce VERB gb19f477842 9 25 organism organism NOUN gb19f477842 9 26 . . PUNCT gb19f477842 10 1 our our PRON gb19f477842 10 2 proposed proposed ADJ gb19f477842 10 3 biosynthetic biosynthetic ADJ gb19f477842 10 4 pathway pathway NOUN gb19f477842 10 5 , , PUNCT gb19f477842 10 6 has have AUX gb19f477842 10 7 been be AUX gb19f477842 10 8 validated validate VERB gb19f477842 10 9 via via ADP gb19f477842 10 10 genetic genetic ADJ gb19f477842 10 11 analysis analysis NOUN gb19f477842 10 12 through through ADP gb19f477842 10 13 complementary complementary ADJ gb19f477842 10 14 use use NOUN gb19f477842 10 15 of of ADP gb19f477842 10 16 whole whole ADJ gb19f477842 10 17 - - PUNCT gb19f477842 10 18 genome genome NOUN gb19f477842 10 19 sequencing sequencing NOUN gb19f477842 10 20 and and CCONJ gb19f477842 10 21 the the DET gb19f477842 10 22 use use NOUN gb19f477842 10 23 of of ADP gb19f477842 10 24 a a DET gb19f477842 10 25 cosmid cosmid PROPN gb19f477842 10 26 library library NOUN gb19f477842 10 27 derived derive VERB gb19f477842 10 28 from from ADP gb19f477842 10 29 the the DET gb19f477842 10 30 myxobacteria myxobacteria PROPN gb19f477842 10 31 strain strain NOUN gb19f477842 10 32 cystobacter cystobacter ADV gb19f477842 10 33 violaceus violaceus NOUN gb19f477842 10 34 strain strain NOUN gb19f477842 10 35 cb cb NOUN gb19f477842 10 36 vi76 vi76 NOUN gb19f477842 10 37 , , PUNCT gb19f477842 10 38 another another DET gb19f477842 10 39 known know VERB gb19f477842 10 40 producer producer NOUN gb19f477842 10 41 of of ADP gb19f477842 10 42 gephyronic gephyronic PROPN gb19f477842 10 43 acid acid PROPN gb19f477842 10 44 . . PUNCT gb19f477842 11 1 the the DET gb19f477842 11 2 gene gene NOUN gb19f477842 11 3 cluster cluster NOUN gb19f477842 11 4 identified identify VERB gb19f477842 11 5 spans span VERB gb19f477842 11 6 52 52 NUM gb19f477842 11 7 kb kb PROPN gb19f477842 11 8 and and CCONJ gb19f477842 11 9 encodes encode VERB gb19f477842 11 10 5 5 NUM gb19f477842 11 11 type type NOUN gb19f477842 11 12 i i PROPN gb19f477842 11 13 polyketide polyketide NOUN gb19f477842 11 14 synthases synthases PROPN gb19f477842 11 15 ( ( PUNCT gb19f477842 11 16 gphfghij gphfghij NOUN gb19f477842 11 17 ) ) PUNCT gb19f477842 11 18 and and CCONJ gb19f477842 11 19 post post ADJ gb19f477842 11 20 - - ADJ gb19f477842 11 21 pks pks ADJ gb19f477842 11 22 modifying modify VERB gb19f477842 11 23 enzymes enzyme NOUN gb19f477842 11 24 including include VERB gb19f477842 11 25 an an DET gb19f477842 11 26 o o NOUN gb19f477842 11 27 - - NOUN gb19f477842 11 28 methyltransferase methyltransferase NOUN gb19f477842 11 29 ( ( PUNCT gb19f477842 11 30 gpha gpha PROPN gb19f477842 11 31 ) ) PUNCT gb19f477842 11 32 and and CCONJ gb19f477842 11 33 cytochrome cytochrome NOUN gb19f477842 11 34 p450 p450 PROPN gb19f477842 11 35 ( ( PUNCT gb19f477842 11 36 gphk gphk PROPN gb19f477842 11 37 ) ) PUNCT gb19f477842 11 38 . . PUNCT gb19f477842 12 1 the the DET gb19f477842 12 2 gephyronic gephyronic PROPN gb19f477842 12 3 acid acid PROPN gb19f477842 12 4 assembly assembly PROPN gb19f477842 12 5 line line NOUN gb19f477842 12 6 also also ADV gb19f477842 12 7 includes include VERB gb19f477842 12 8 several several ADJ gb19f477842 12 9 unique unique ADJ gb19f477842 12 10 features feature NOUN gb19f477842 12 11 , , PUNCT gb19f477842 12 12 such such ADJ gb19f477842 12 13 as as ADP gb19f477842 12 14 methyl methyl NOUN gb19f477842 12 15 branch branch NOUN gb19f477842 12 16 incorporation incorporation NOUN gb19f477842 12 17 from from ADP gb19f477842 12 18 s s NOUN gb19f477842 12 19 - - PUNCT gb19f477842 12 20 adenosyl adenosyl NOUN gb19f477842 12 21 - - PUNCT gb19f477842 12 22 l l NOUN gb19f477842 12 23 - - PUNCT gb19f477842 12 24 methionine methionine NOUN gb19f477842 12 25 ( ( PUNCT gb19f477842 12 26 sam sam PROPN gb19f477842 12 27 ) ) PUNCT gb19f477842 12 28 by by ADP gb19f477842 12 29 the the DET gb19f477842 12 30 action action NOUN gb19f477842 12 31 of of ADP gb19f477842 12 32 sam sam PROPN gb19f477842 12 33 - - PUNCT gb19f477842 12 34 dependent dependent ADJ gb19f477842 12 35 methyltransferases methyltransferase NOUN gb19f477842 12 36 and and CCONJ gb19f477842 12 37 the the DET gb19f477842 12 38 presence presence NOUN gb19f477842 12 39 of of ADP gb19f477842 12 40 a a DET gb19f477842 12 41 unique unique ADJ gb19f477842 12 42 loading loading NOUN gb19f477842 12 43 domain domain NOUN gb19f477842 12 44 . . PUNCT gb19f477842 13 1 targeted target VERB gb19f477842 13 2 gene gene NOUN gb19f477842 13 3 inactivation inactivation NOUN gb19f477842 13 4 of of ADP gb19f477842 13 5 key key ADJ gb19f477842 13 6 genes gene NOUN gb19f477842 13 7 resulted result VERB gb19f477842 13 8 in in ADP gb19f477842 13 9 deactivation deactivation NOUN gb19f477842 13 10 of of ADP gb19f477842 13 11 the the DET gb19f477842 13 12 biosynthetic biosynthetic ADJ gb19f477842 13 13 pathway pathway NOUN gb19f477842 13 14 , , PUNCT gb19f477842 13 15 providing provide VERB gb19f477842 13 16 direct direct ADJ gb19f477842 13 17 evidence evidence NOUN gb19f477842 13 18 for for ADP gb19f477842 13 19 its its PRON gb19f477842 13 20 isolation isolation NOUN gb19f477842 13 21 and and CCONJ gb19f477842 13 22 characterization characterization NOUN gb19f477842 13 23 . . PUNCT gb19f477842 14 1 identification identification NOUN gb19f477842 14 2 of of ADP gb19f477842 14 3 the the DET gb19f477842 14 4 gephyronic gephyronic PROPN gb19f477842 14 5 acid acid PROPN gb19f477842 14 6 gene gene NOUN gb19f477842 14 7 cluster cluster NOUN gb19f477842 14 8 is be AUX gb19f477842 14 9 the the DET gb19f477842 14 10 key key ADJ gb19f477842 14 11 step step NOUN gb19f477842 14 12 in in ADP gb19f477842 14 13 a a DET gb19f477842 14 14 heterologous heterologous ADJ gb19f477842 14 15 expression expression NOUN gb19f477842 14 16 system system NOUN gb19f477842 14 17 , , PUNCT gb19f477842 14 18 which which PRON gb19f477842 14 19 will will AUX gb19f477842 14 20 provide provide VERB gb19f477842 14 21 an an DET gb19f477842 14 22 alternative alternative ADJ gb19f477842 14 23 source source NOUN gb19f477842 14 24 of of ADP gb19f477842 14 25 the the DET gb19f477842 14 26 natural natural ADJ gb19f477842 14 27 product product NOUN gb19f477842 14 28 and and CCONJ gb19f477842 14 29 a a DET gb19f477842 14 30 fermentation fermentation NOUN gb19f477842 14 31 system system NOUN gb19f477842 14 32 for for ADP gb19f477842 14 33 precursor precursor PROPN gb19f477842 14 34 directed direct VERB gb19f477842 14 35 biosynthesis biosynthesis NOUN gb19f477842 14 36 of of ADP gb19f477842 14 37 gephyronic gephyronic PROPN gb19f477842 14 38 acid acid PROPN gb19f477842 14 39 analogues analogue NOUN gb19f477842 14 40 . . PUNCT