id sid tid token lemma pos g445cc10q3t 1 1 newly newly ADV g445cc10q3t 1 2 synthesized synthesize VERB g445cc10q3t 1 3 proteins protein NOUN g445cc10q3t 1 4 must must AUX g445cc10q3t 1 5 form form VERB g445cc10q3t 1 6 their their PRON g445cc10q3t 1 7 native native ADJ g445cc10q3t 1 8 structure structure NOUN g445cc10q3t 1 9 in in ADP g445cc10q3t 1 10 the the DET g445cc10q3t 1 11 crowded crowded ADJ g445cc10q3t 1 12 environment environment NOUN g445cc10q3t 1 13 of of ADP g445cc10q3t 1 14 the the DET g445cc10q3t 1 15 cell cell NOUN g445cc10q3t 1 16 , , PUNCT g445cc10q3t 1 17 while while SCONJ g445cc10q3t 1 18 avoiding avoid VERB g445cc10q3t 1 19 non non ADJ g445cc10q3t 1 20 - - ADJ g445cc10q3t 1 21 native native ADJ g445cc10q3t 1 22 conformations conformation NOUN g445cc10q3t 1 23 that that PRON g445cc10q3t 1 24 can can AUX g445cc10q3t 1 25 lead lead VERB g445cc10q3t 1 26 to to ADP g445cc10q3t 1 27 aggregation aggregation NOUN g445cc10q3t 1 28 . . PUNCT g445cc10q3t 2 1 p22 p22 PROPN g445cc10q3t 2 2 tailspike tailspike PROPN g445cc10q3t 2 3 is be AUX g445cc10q3t 2 4 a a DET g445cc10q3t 2 5 homotrimer homotrimer X g445cc10q3t 2 6 prone prone ADJ g445cc10q3t 2 7 to to ADP g445cc10q3t 2 8 aggregation aggregation NOUN g445cc10q3t 2 9 via via ADP g445cc10q3t 2 10 misfolding misfolding NOUN g445cc10q3t 2 11 of of ADP g445cc10q3t 2 12 its its PRON g445cc10q3t 2 13 central central ADJ g445cc10q3t 2 14 beta beta NOUN g445cc10q3t 2 15 - - PUNCT g445cc10q3t 2 16 helix helix NOUN g445cc10q3t 2 17 domain domain NOUN g445cc10q3t 2 18 in in ADP g445cc10q3t 2 19 vitro vitro X g445cc10q3t 2 20 . . PUNCT g445cc10q3t 3 1 to to PART g445cc10q3t 3 2 assess assess VERB g445cc10q3t 3 3 whether whether SCONJ g445cc10q3t 3 4 co co ADJ g445cc10q3t 3 5 - - ADJ g445cc10q3t 3 6 translational translational ADJ g445cc10q3t 3 7 folding folding NOUN g445cc10q3t 3 8 enables enable VERB g445cc10q3t 3 9 newly newly ADV g445cc10q3t 3 10 synthesized synthesize VERB g445cc10q3t 3 11 tailspike tailspike ADJ g445cc10q3t 3 12 chains chain NOUN g445cc10q3t 3 13 to to PART g445cc10q3t 3 14 avoid avoid VERB g445cc10q3t 3 15 aggregation aggregation NOUN g445cc10q3t 3 16 - - PUNCT g445cc10q3t 3 17 prone prone ADJ g445cc10q3t 3 18 conformations conformation NOUN g445cc10q3t 3 19 in in ADP g445cc10q3t 3 20 vivo vivo PROPN g445cc10q3t 3 21 , , PUNCT g445cc10q3t 3 22 a a DET g445cc10q3t 3 23 novel novel ADJ g445cc10q3t 3 24 method method NOUN g445cc10q3t 3 25 was be AUX g445cc10q3t 3 26 first first ADV g445cc10q3t 3 27 developed develop VERB g445cc10q3t 3 28 to to PART g445cc10q3t 3 29 produce produce VERB g445cc10q3t 3 30 stalled stall VERB g445cc10q3t 3 31 ribosome ribosome ADJ g445cc10q3t 3 32 nascent nascent ADJ g445cc10q3t 3 33 chain chain NOUN g445cc10q3t 3 34 complexes complex NOUN g445cc10q3t 3 35 . . PUNCT g445cc10q3t 4 1 this this DET g445cc10q3t 4 2 new new ADJ g445cc10q3t 4 3 method method NOUN g445cc10q3t 4 4 was be AUX g445cc10q3t 4 5 used use VERB g445cc10q3t 4 6 to to PART g445cc10q3t 4 7 measure measure VERB g445cc10q3t 4 8 anti anti ADJ g445cc10q3t 4 9 - - ADJ g445cc10q3t 4 10 tailspike tailspike ADJ g445cc10q3t 4 11 monoclonal monoclonal NOUN g445cc10q3t 4 12 antibody antibody NOUN g445cc10q3t 4 13 binding bind VERB g445cc10q3t 4 14 to to ADP g445cc10q3t 4 15 and and CCONJ g445cc10q3t 4 16 partial partial ADJ g445cc10q3t 4 17 protease protease NOUN g445cc10q3t 4 18 digestion digestion NOUN g445cc10q3t 4 19 of of ADP g445cc10q3t 4 20 four four NUM g445cc10q3t 4 21 different different ADJ g445cc10q3t 4 22 tailspike tailspike ADJ g445cc10q3t 4 23 nascent nascent ADJ g445cc10q3t 4 24 chain chain NOUN g445cc10q3t 4 25 lengths length NOUN g445cc10q3t 4 26 . . PUNCT g445cc10q3t 5 1 these these DET g445cc10q3t 5 2 experiments experiment NOUN g445cc10q3t 5 3 reveal reveal VERB g445cc10q3t 5 4 ribosome ribosome ADJ g445cc10q3t 5 5 - - PUNCT g445cc10q3t 5 6 bound bind VERB g445cc10q3t 5 7 nascent nascent ADJ g445cc10q3t 5 8 tailspike tailspike PROPN g445cc10q3t 5 9 chains chain NOUN g445cc10q3t 5 10 populate populate VERB g445cc10q3t 5 11 ordered order VERB g445cc10q3t 5 12 conformations conformation NOUN g445cc10q3t 5 13 with with ADP g445cc10q3t 5 14 some some DET g445cc10q3t 5 15 native native ADJ g445cc10q3t 5 16 - - PUNCT g445cc10q3t 5 17 state state NOUN g445cc10q3t 5 18 structural structural ADJ g445cc10q3t 5 19 features feature NOUN g445cc10q3t 5 20 , , PUNCT g445cc10q3t 5 21 but but CCONJ g445cc10q3t 5 22 these these DET g445cc10q3t 5 23 conformations conformation NOUN g445cc10q3t 5 24 are be AUX g445cc10q3t 5 25 distinct distinct ADJ g445cc10q3t 5 26 from from ADP g445cc10q3t 5 27 the the DET g445cc10q3t 5 28 predominant predominant ADJ g445cc10q3t 5 29 conformations conformation NOUN g445cc10q3t 5 30 of of ADP g445cc10q3t 5 31 tailspike tailspike PROPN g445cc10q3t 5 32 in in ADP g445cc10q3t 5 33 vitro vitro X g445cc10q3t 5 34 refolding refolding NOUN g445cc10q3t 5 35 intermediates intermediate NOUN g445cc10q3t 5 36 and and CCONJ g445cc10q3t 5 37 refolded refolde VERB g445cc10q3t 5 38 , , PUNCT g445cc10q3t 5 39 unstalled unstalled ADJ g445cc10q3t 5 40 tailspike tailspike ADJ g445cc10q3t 5 41 truncations truncation NOUN g445cc10q3t 5 42 . . PUNCT g445cc10q3t 6 1 these these DET g445cc10q3t 6 2 results result NOUN g445cc10q3t 6 3 suggest suggest VERB g445cc10q3t 6 4 the the DET g445cc10q3t 6 5 aggregation aggregation NOUN g445cc10q3t 6 6 - - PUNCT g445cc10q3t 6 7 prone prone ADJ g445cc10q3t 6 8 beta beta NOUN g445cc10q3t 6 9 - - PUNCT g445cc10q3t 6 10 helix helix NOUN g445cc10q3t 6 11 domain domain NOUN g445cc10q3t 6 12 pre pre VERB g445cc10q3t 6 13 - - ADJ g445cc10q3t 6 14 organizes organize VERB g445cc10q3t 6 15 co co NOUN g445cc10q3t 6 16 - - NOUN g445cc10q3t 6 17 translationally translationally ADJ g445cc10q3t 6 18 , , PUNCT g445cc10q3t 6 19 prior prior ADV g445cc10q3t 6 20 to to ADP g445cc10q3t 6 21 chain chain NOUN g445cc10q3t 6 22 release release NOUN g445cc10q3t 6 23 , , PUNCT g445cc10q3t 6 24 and and CCONJ g445cc10q3t 6 25 that that SCONJ g445cc10q3t 6 26 this this DET g445cc10q3t 6 27 conformation conformation NOUN g445cc10q3t 6 28 is be AUX g445cc10q3t 6 29 distinct distinct ADJ g445cc10q3t 6 30 from from ADP g445cc10q3t 6 31 the the DET g445cc10q3t 6 32 global global ADJ g445cc10q3t 6 33 energy energy NOUN g445cc10q3t 6 34 minimum minimum NOUN g445cc10q3t 6 35 for for ADP g445cc10q3t 6 36 the the DET g445cc10q3t 6 37 truncated truncated ADJ g445cc10q3t 6 38 free free ADJ g445cc10q3t 6 39 chain chain NOUN g445cc10q3t 6 40 in in ADP g445cc10q3t 6 41 solution solution NOUN g445cc10q3t 6 42 . . PUNCT