id sid tid token lemma pos 8910js9770s 1 1 the the DET 8910js9770s 1 2 piggybac piggybac NOUN 8910js9770s 1 3 mobile mobile ADJ 8910js9770s 1 4 element element NOUN 8910js9770s 1 5 is be AUX 8910js9770s 1 6 quickly quickly ADV 8910js9770s 1 7 gaining gain VERB 8910js9770s 1 8 popularity popularity NOUN 8910js9770s 1 9 as as ADP 8910js9770s 1 10 a a DET 8910js9770s 1 11 tool tool NOUN 8910js9770s 1 12 for for ADP 8910js9770s 1 13 the the DET 8910js9770s 1 14 transgenesis transgenesis NOUN 8910js9770s 1 15 of of ADP 8910js9770s 1 16 many many ADJ 8910js9770s 1 17 eukaryotic eukaryotic ADJ 8910js9770s 1 18 organisms organism NOUN 8910js9770s 1 19 . . PUNCT 8910js9770s 2 1 by by ADP 8910js9770s 2 2 studying study VERB 8910js9770s 2 3 the the DET 8910js9770s 2 4 transposase transposase NOUN 8910js9770s 2 5 ( ( PUNCT 8910js9770s 2 6 tpase tpase NOUN 8910js9770s 2 7 ) ) PUNCT 8910js9770s 2 8 which which PRON 8910js9770s 2 9 catalyzes catalyze VERB 8910js9770s 2 10 the the DET 8910js9770s 2 11 movement movement NOUN 8910js9770s 2 12 of of ADP 8910js9770s 2 13 piggybac piggybac NOUN 8910js9770s 2 14 , , PUNCT 8910js9770s 2 15 modification modification NOUN 8910js9770s 2 16 of of ADP 8910js9770s 2 17 this this DET 8910js9770s 2 18 vector vector NOUN 8910js9770s 2 19 system system NOUN 8910js9770s 2 20 may may AUX 8910js9770s 2 21 be be AUX 8910js9770s 2 22 possible possible ADJ 8910js9770s 2 23 in in ADP 8910js9770s 2 24 order order NOUN 8910js9770s 2 25 to to PART 8910js9770s 2 26 make make VERB 8910js9770s 2 27 it it PRON 8910js9770s 2 28 a a DET 8910js9770s 2 29 more more ADV 8910js9770s 2 30 effective effective ADJ 8910js9770s 2 31 transgenesis transgenesis NOUN 8910js9770s 2 32 tool tool NOUN 8910js9770s 2 33 . . PUNCT 8910js9770s 3 1 in in ADP 8910js9770s 3 2 a a DET 8910js9770s 3 3 previous previous ADJ 8910js9770s 3 4 publication publication NOUN 8910js9770s 3 5 ( ( PUNCT 8910js9770s 3 6 sarkar sarkar PROPN 8910js9770s 3 7 et et PROPN 8910js9770s 3 8 al al PROPN 8910js9770s 3 9 . . PROPN 8910js9770s 3 10 , , PUNCT 8910js9770s 3 11 2003 2003 NUM 8910js9770s 3 12 ) ) PUNCT 8910js9770s 3 13 proposed propose VERB 8910js9770s 3 14 the the DET 8910js9770s 3 15 presence presence NOUN 8910js9770s 3 16 of of ADP 8910js9770s 3 17 the the DET 8910js9770s 3 18 widespread widespread ADJ 8910js9770s 3 19 ' ' PUNCT 8910js9770s 3 20 dde dde X 8910js9770s 3 21 / / SYM 8910js9770s 3 22 ddd ddd PROPN 8910js9770s 3 23 ' ' PART 8910js9770s 3 24 motif motif NOUN 8910js9770s 3 25 for for ADP 8910js9770s 3 26 piggybac piggybac NOUN 8910js9770s 3 27 at at ADP 8910js9770s 3 28 amino amino NOUN 8910js9770s 3 29 acid acid NOUN 8910js9770s 3 30 positions position NOUN 8910js9770s 3 31 d268 d268 PROPN 8910js9770s 3 32 , , PUNCT 8910js9770s 3 33 d346 d346 PROPN 8910js9770s 3 34 , , PUNCT 8910js9770s 3 35 and and CCONJ 8910js9770s 3 36 d447 d447 PROPN 8910js9770s 3 37 . . PUNCT 8910js9770s 4 1 a a DET 8910js9770s 4 2 psortii psortii ADJ 8910js9770s 4 3 analysis analysis NOUN 8910js9770s 4 4 of of ADP 8910js9770s 4 5 the the DET 8910js9770s 4 6 tpase tpase PROPN 8910js9770s 4 7 amino amino NOUN 8910js9770s 4 8 acid acid NOUN 8910js9770s 4 9 sequence sequence NOUN 8910js9770s 4 10 predicts predict VERB 8910js9770s 4 11 several several ADJ 8910js9770s 4 12 nuclear nuclear ADJ 8910js9770s 4 13 localization localization NOUN 8910js9770s 4 14 signals signal NOUN 8910js9770s 4 15 ( ( PUNCT 8910js9770s 4 16 nls nls NOUN 8910js9770s 4 17 ) ) PUNCT 8910js9770s 4 18 near near ADP 8910js9770s 4 19 the the DET 8910js9770s 4 20 c c NOUN 8910js9770s 4 21 - - PUNCT 8910js9770s 4 22 terminus terminus NOUN 8910js9770s 4 23 , , PUNCT 8910js9770s 4 24 just just ADV 8910js9770s 4 25 upstream upstream ADV 8910js9770s 4 26 of of ADP 8910js9770s 4 27 a a DET 8910js9770s 4 28 putative putative ADJ 8910js9770s 4 29 zinc zinc NOUN 8910js9770s 4 30 finger finger NOUN 8910js9770s 4 31 ( ( PUNCT 8910js9770s 4 32 znf znf NOUN 8910js9770s 4 33 ) ) PUNCT 8910js9770s 4 34 . . PUNCT 8910js9770s 5 1 this this DET 8910js9770s 5 2 study study NOUN 8910js9770s 5 3 utilizes utilize VERB 8910js9770s 5 4 directed directed ADJ 8910js9770s 5 5 mutagenesis mutagenesis NOUN 8910js9770s 5 6 and and CCONJ 8910js9770s 5 7 plasmid plasmid ADJ 8910js9770s 5 8 - - PUNCT 8910js9770s 5 9 based base VERB 8910js9770s 5 10 mobility mobility NOUN 8910js9770s 5 11 assays assay VERB 8910js9770s 5 12 to to PART 8910js9770s 5 13 assess assess VERB 8910js9770s 5 14 the the DET 8910js9770s 5 15 importance importance NOUN 8910js9770s 5 16 of of ADP 8910js9770s 5 17 conserved conserved ADJ 8910js9770s 5 18 aspartate aspartate NOUN 8910js9770s 5 19 residues residue NOUN 8910js9770s 5 20 as as ADP 8910js9770s 5 21 the the DET 8910js9770s 5 22 catalytic catalytic ADJ 8910js9770s 5 23 core core NOUN 8910js9770s 5 24 of of ADP 8910js9770s 5 25 the the DET 8910js9770s 5 26 piggybac piggybac NOUN 8910js9770s 5 27 tpase tpase NOUN 8910js9770s 5 28 . . PUNCT 8910js9770s 6 1 individual individual ADJ 8910js9770s 6 2 point point NOUN 8910js9770s 6 3 - - PUNCT 8910js9770s 6 4 mutations mutation NOUN 8910js9770s 6 5 have have AUX 8910js9770s 6 6 been be AUX 8910js9770s 6 7 functionally functionally ADV 8910js9770s 6 8 analyzed analyze VERB 8910js9770s 6 9 with with ADP 8910js9770s 6 10 respect respect NOUN 8910js9770s 6 11 to to ADP 8910js9770s 6 12 charge charge NOUN 8910js9770s 6 13 and and CCONJ 8910js9770s 6 14 physical physical ADJ 8910js9770s 6 15 size size NOUN 8910js9770s 6 16 in in ADP 8910js9770s 6 17 all all DET 8910js9770s 6 18 three three NUM 8910js9770s 6 19 proposed propose VERB 8910js9770s 6 20 residues residue NOUN 8910js9770s 6 21 of of ADP 8910js9770s 6 22 the the DET 8910js9770s 6 23 ' ' PUNCT 8910js9770s 6 24 ddd ddd PROPN 8910js9770s 6 25 ' ' PUNCT 8910js9770s 6 26 motif motif NOUN 8910js9770s 6 27 , , PUNCT 8910js9770s 6 28 as as ADV 8910js9770s 6 29 well well ADV 8910js9770s 6 30 as as ADP 8910js9770s 6 31 another another DET 8910js9770s 6 32 nearby nearby ADV 8910js9770s 6 33 , , PUNCT 8910js9770s 6 34 highly highly ADV 8910js9770s 6 35 conserved conserved ADJ 8910js9770s 6 36 aspartate aspartate NOUN 8910js9770s 6 37 at at ADP 8910js9770s 6 38 d450 d450 PROPN 8910js9770s 6 39 . . PROPN 8910js9770s 6 40 results result NOUN 8910js9770s 6 41 indicate indicate VERB 8910js9770s 6 42 that that SCONJ 8910js9770s 6 43 all all DET 8910js9770s 6 44 four four NUM 8910js9770s 6 45 aspartates aspartate NOUN 8910js9770s 6 46 are be AUX 8910js9770s 6 47 necessary necessary ADJ 8910js9770s 6 48 , , PUNCT 8910js9770s 6 49 to to ADP 8910js9770s 6 50 one one NUM 8910js9770s 6 51 degree degree NOUN 8910js9770s 6 52 or or CCONJ 8910js9770s 6 53 another another PRON 8910js9770s 6 54 , , PUNCT 8910js9770s 6 55 for for SCONJ 8910js9770s 6 56 excision excision NOUN 8910js9770s 6 57 to to PART 8910js9770s 6 58 occur occur VERB 8910js9770s 6 59 in in ADP 8910js9770s 6 60 a a DET 8910js9770s 6 61 cellular cellular ADJ 8910js9770s 6 62 environment environment NOUN 8910js9770s 6 63 , , PUNCT 8910js9770s 6 64 but but CCONJ 8910js9770s 6 65 d450 d450 PROPN 8910js9770s 6 66 seems seem VERB 8910js9770s 6 67 to to PART 8910js9770s 6 68 have have VERB 8910js9770s 6 69 a a DET 8910js9770s 6 70 tolerance tolerance NOUN 8910js9770s 6 71 for for ADP 8910js9770s 6 72 a a DET 8910js9770s 6 73 glutamate glutamate NOUN 8910js9770s 6 74 substitution substitution NOUN 8910js9770s 6 75 . . PUNCT 8910js9770s 7 1 the the DET 8910js9770s 7 2 piggybac piggybac NOUN 8910js9770s 7 3 tpase tpase NOUN 8910js9770s 7 4 was be AUX 8910js9770s 7 5 fused fuse VERB 8910js9770s 7 6 upstream upstream ADJ 8910js9770s 7 7 and and CCONJ 8910js9770s 7 8 in in ADP 8910js9770s 7 9 - - PUNCT 8910js9770s 7 10 frame frame NOUN 8910js9770s 7 11 with with ADP 8910js9770s 7 12 the the DET 8910js9770s 7 13 enhanced enhanced ADJ 8910js9770s 7 14 yellow yellow ADJ 8910js9770s 7 15 fluorescent fluorescent NOUN 8910js9770s 7 16 protein protein NOUN 8910js9770s 7 17 ( ( PUNCT 8910js9770s 7 18 eyfp eyfp PROPN 8910js9770s 7 19 ) ) PUNCT 8910js9770s 7 20 in in ADP 8910js9770s 7 21 the the DET 8910js9770s 7 22 drosophila drosophila NOUN 8910js9770s 7 23 melanogaster melanogaster NOUN 8910js9770s 7 24 inducible inducible VERB 8910js9770s 7 25 metallothionein metallothionein NOUN 8910js9770s 7 26 ( ( PUNCT 8910js9770s 7 27 mt mt PROPN 8910js9770s 7 28 ) ) PUNCT 8910js9770s 7 29 promoter promoter NOUN 8910js9770s 7 30 and and CCONJ 8910js9770s 7 31 the the DET 8910js9770s 7 32 fluorescence fluorescence NOUN 8910js9770s 7 33 tracked track VERB 8910js9770s 7 34 by by ADP 8910js9770s 7 35 confocal confocal ADJ 8910js9770s 7 36 microscopy microscopy NOUN 8910js9770s 7 37 . . PUNCT 8910js9770s 8 1 through through ADP 8910js9770s 8 2 n n NOUN 8910js9770s 8 3 and and CCONJ 8910js9770s 8 4 c c X 8910js9770s 8 5 - - PUNCT 8910js9770s 8 6 terminal terminal ADJ 8910js9770s 8 7 truncations truncation NOUN 8910js9770s 8 8 , , PUNCT 8910js9770s 8 9 targeted target VERB 8910js9770s 8 10 internal internal ADJ 8910js9770s 8 11 deletions deletion NOUN 8910js9770s 8 12 , , PUNCT 8910js9770s 8 13 and and CCONJ 8910js9770s 8 14 specific specific ADJ 8910js9770s 8 15 amino amino ADJ 8910js9770s 8 16 acid acid NOUN 8910js9770s 8 17 mutations mutation NOUN 8910js9770s 8 18 of of ADP 8910js9770s 8 19 the the DET 8910js9770s 8 20 piggybac piggybac NOUN 8910js9770s 8 21 tpase tpase NOUN 8910js9770s 8 22 open open ADJ 8910js9770s 8 23 reading reading NOUN 8910js9770s 8 24 frame frame NOUN 8910js9770s 8 25 ( ( PUNCT 8910js9770s 8 26 orf orf PROPN 8910js9770s 8 27 ) ) PUNCT 8910js9770s 8 28 , , PUNCT 8910js9770s 8 29 the the DET 8910js9770s 8 30 region region NOUN 8910js9770s 8 31 containing contain VERB 8910js9770s 8 32 the the DET 8910js9770s 8 33 psortii psortii NOUN 8910js9770s 8 34 predicted predict VERB 8910js9770s 8 35 nlss nlss PROPN 8910js9770s 8 36 was be AUX 8910js9770s 8 37 determined determined ADJ 8910js9770s 8 38 to to PART 8910js9770s 8 39 be be AUX 8910js9770s 8 40 required require VERB 8910js9770s 8 41 for for SCONJ 8910js9770s 8 42 the the DET 8910js9770s 8 43 tpase tpase NOUN 8910js9770s 8 44 to to PART 8910js9770s 8 45 enter enter VERB 8910js9770s 8 46 the the DET 8910js9770s 8 47 nucleus nucleus NOUN 8910js9770s 8 48 of of ADP 8910js9770s 8 49 s2 s2 PROPN 8910js9770s 8 50 cells cell NOUN 8910js9770s 8 51 ; ; PUNCT 8910js9770s 8 52 other other ADJ 8910js9770s 8 53 additional additional ADJ 8910js9770s 8 54 negatively negatively ADV 8910js9770s 8 55 charged charge VERB 8910js9770s 8 56 amino amino NOUN 8910js9770s 8 57 acids acid NOUN 8910js9770s 8 58 a a DET 8910js9770s 8 59 short short ADJ 8910js9770s 8 60 length length NOUN 8910js9770s 8 61 upstream upstream ADV 8910js9770s 8 62 of of ADP 8910js9770s 8 63 this this DET 8910js9770s 8 64 region region NOUN 8910js9770s 8 65 were be AUX 8910js9770s 8 66 also also ADV 8910js9770s 8 67 required require VERB 8910js9770s 8 68 for for ADP 8910js9770s 8 69 proper proper ADJ 8910js9770s 8 70 function function NOUN 8910js9770s 8 71 of of ADP 8910js9770s 8 72 the the DET 8910js9770s 8 73 nls nls PROPN 8910js9770s 8 74 . . PUNCT 8910js9770s 8 75 control control PROPN 8910js9770s 8 76 of of ADP 8910js9770s 8 77 the the DET 8910js9770s 8 78 spread spread NOUN 8910js9770s 8 79 of of ADP 8910js9770s 8 80 dengue dengue NOUN 8910js9770s 8 81 virus virus NOUN 8910js9770s 8 82 ( ( PUNCT 8910js9770s 8 83 denv denv NOUN 8910js9770s 8 84 ) ) PUNCT 8910js9770s 8 85 has have AUX 8910js9770s 8 86 become become VERB 8910js9770s 8 87 a a DET 8910js9770s 8 88 more more ADV 8910js9770s 8 89 important important ADJ 8910js9770s 8 90 concern concern NOUN 8910js9770s 8 91 , , PUNCT 8910js9770s 8 92 with with ADP 8910js9770s 8 93 the the DET 8910js9770s 8 94 growth growth NOUN 8910js9770s 8 95 of of ADP 8910js9770s 8 96 worldwide worldwide ADJ 8910js9770s 8 97 travel travel NOUN 8910js9770s 8 98 and and CCONJ 8910js9770s 8 99 development development NOUN 8910js9770s 8 100 of of ADP 8910js9770s 8 101 rural rural ADJ 8910js9770s 8 102 areas area NOUN 8910js9770s 8 103 . . PUNCT 8910js9770s 9 1 recent recent ADJ 8910js9770s 9 2 outbreaks outbreak NOUN 8910js9770s 9 3 in in ADP 8910js9770s 9 4 the the DET 8910js9770s 9 5 third third ADJ 8910js9770s 9 6 world world NOUN 8910js9770s 9 7 have have AUX 8910js9770s 9 8 taken take VERB 8910js9770s 9 9 a a DET 8910js9770s 9 10 heavy heavy ADJ 8910js9770s 9 11 economic economic ADJ 8910js9770s 9 12 toll toll NOUN 8910js9770s 9 13 on on ADP 8910js9770s 9 14 developing develop VERB 8910js9770s 9 15 countries country NOUN 8910js9770s 9 16 in in ADP 8910js9770s 9 17 terms term NOUN 8910js9770s 9 18 of of ADP 8910js9770s 9 19 morbidity morbidity NOUN 8910js9770s 9 20 . . PUNCT 8910js9770s 10 1 an an DET 8910js9770s 10 2 effective effective ADJ 8910js9770s 10 3 vaccine vaccine NOUN 8910js9770s 10 4 against against ADP 8910js9770s 10 5 all all DET 8910js9770s 10 6 four four NUM 8910js9770s 10 7 serotypes serotype NOUN 8910js9770s 10 8 of of ADP 8910js9770s 10 9 the the DET 8910js9770s 10 10 virus virus NOUN 8910js9770s 10 11 has have AUX 8910js9770s 10 12 remained remain VERB 8910js9770s 10 13 elusive elusive ADJ 8910js9770s 10 14 due due ADP 8910js9770s 10 15 to to ADP 8910js9770s 10 16 antibody antibody NOUN 8910js9770s 10 17 dependent dependent ADJ 8910js9770s 10 18 enhancement enhancement NOUN 8910js9770s 10 19 of of ADP 8910js9770s 10 20 a a DET 8910js9770s 10 21 viral viral ADJ 8910js9770s 10 22 infection infection NOUN 8910js9770s 10 23 . . PUNCT 8910js9770s 11 1 one one NUM 8910js9770s 11 2 alternative alternative NOUN 8910js9770s 11 3 to to ADP 8910js9770s 11 4 vaccines vaccine NOUN 8910js9770s 11 5 currently currently ADV 8910js9770s 11 6 being be AUX 8910js9770s 11 7 explored explore VERB 8910js9770s 11 8 is be AUX 8910js9770s 11 9 the the DET 8910js9770s 11 10 creation creation NOUN 8910js9770s 11 11 of of ADP 8910js9770s 11 12 mosquitoes mosquito NOUN 8910js9770s 11 13 unable unable ADJ 8910js9770s 11 14 to to PART 8910js9770s 11 15 spread spread VERB 8910js9770s 11 16 the the DET 8910js9770s 11 17 virus virus NOUN 8910js9770s 11 18 from from ADP 8910js9770s 11 19 person person NOUN 8910js9770s 11 20 to to ADP 8910js9770s 11 21 person person NOUN 8910js9770s 11 22 . . PUNCT 8910js9770s 12 1 the the DET 8910js9770s 12 2 establishment establishment NOUN 8910js9770s 12 3 and and CCONJ 8910js9770s 12 4 spread spread VERB 8910js9770s 12 5 of of ADP 8910js9770s 12 6 a a DET 8910js9770s 12 7 so so ADV 8910js9770s 12 8 called call VERB 8910js9770s 12 9 refractory refractory ADJ 8910js9770s 12 10 strain strain NOUN 8910js9770s 12 11 would would AUX 8910js9770s 12 12 effectively effectively ADV 8910js9770s 12 13 halt halt VERB 8910js9770s 12 14 the the DET 8910js9770s 12 15 denv denv ADJ 8910js9770s 12 16 life life NOUN 8910js9770s 12 17 cycle cycle NOUN 8910js9770s 12 18 in in ADP 8910js9770s 12 19 its its PRON 8910js9770s 12 20 tracks track NOUN 8910js9770s 12 21 . . PUNCT 8910js9770s 13 1 obviously obviously ADV 8910js9770s 13 2 , , PUNCT 8910js9770s 13 3 a a DET 8910js9770s 13 4 key key ADJ 8910js9770s 13 5 element element NOUN 8910js9770s 13 6 of of ADP 8910js9770s 13 7 this this DET 8910js9770s 13 8 population population NOUN 8910js9770s 13 9 replacement replacement NOUN 8910js9770s 13 10 approach approach NOUN 8910js9770s 13 11 is be AUX 8910js9770s 13 12 an an DET 8910js9770s 13 13 effective effective ADJ 8910js9770s 13 14 transgene transgene NOUN 8910js9770s 13 15 conferring confer VERB 8910js9770s 13 16 resistance resistance NOUN 8910js9770s 13 17 to to ADP 8910js9770s 13 18 viral viral ADJ 8910js9770s 13 19 transmission transmission NOUN 8910js9770s 13 20 . . PUNCT 8910js9770s 14 1 the the DET 8910js9770s 14 2 ability ability NOUN 8910js9770s 14 3 to to PART 8910js9770s 14 4 precisely precisely ADV 8910js9770s 14 5 target target VERB 8910js9770s 14 6 the the DET 8910js9770s 14 7 transgene transgene NOUN 8910js9770s 14 8 to to ADP 8910js9770s 14 9 invariant invariant ADJ 8910js9770s 14 10 elements element NOUN 8910js9770s 14 11 of of ADP 8910js9770s 14 12 the the DET 8910js9770s 14 13 virus virus NOUN 8910js9770s 14 14 would would AUX 8910js9770s 14 15 make make VERB 8910js9770s 14 16 it it PRON 8910js9770s 14 17 more more ADV 8910js9770s 14 18 effective effective ADJ 8910js9770s 14 19 against against ADP 8910js9770s 14 20 a a DET 8910js9770s 14 21 wider wide ADJ 8910js9770s 14 22 variety variety NOUN 8910js9770s 14 23 of of ADP 8910js9770s 14 24 serotypes serotype NOUN 8910js9770s 14 25 and and CCONJ 8910js9770s 14 26 strains strain NOUN 8910js9770s 14 27 . . PUNCT 8910js9770s 15 1 the the DET 8910js9770s 15 2 ability ability NOUN 8910js9770s 15 3 of of ADP 8910js9770s 15 4 the the DET 8910js9770s 15 5 tetrahymena tetrahymena NOUN 8910js9770s 15 6 thermophila thermophila NOUN 8910js9770s 15 7 group group NOUN 8910js9770s 15 8 i i PRON 8910js9770s 15 9 intron intron VERB 8910js9770s 15 10 to to ADP 8910js9770s 15 11 catalyze catalyze PROPN 8910js9770s 15 12 the the DET 8910js9770s 15 13 trans trans NOUN 8910js9770s 15 14 - - NOUN 8910js9770s 15 15 splicing splicing NOUN 8910js9770s 15 16 of of ADP 8910js9770s 15 17 subgenomic subgenomic ADJ 8910js9770s 15 18 sequences sequence NOUN 8910js9770s 15 19 highly highly ADV 8910js9770s 15 20 conserved conserve VERB 8910js9770s 15 21 in in ADP 8910js9770s 15 22 the the DET 8910js9770s 15 23 dengue dengue NOUN 8910js9770s 15 24 genome genome NOUN 8910js9770s 15 25 across across ADP 8910js9770s 15 26 all all DET 8910js9770s 15 27 serotypes serotype NOUN 8910js9770s 15 28 was be AUX 8910js9770s 15 29 tested test VERB 8910js9770s 15 30 . . PUNCT 8910js9770s 16 1 by by ADP 8910js9770s 16 2 specifically specifically ADV 8910js9770s 16 3 targeting target VERB 8910js9770s 16 4 these these DET 8910js9770s 16 5 regions region NOUN 8910js9770s 16 6 , , PUNCT 8910js9770s 16 7 trans trans ADJ 8910js9770s 16 8 - - PUNCT 8910js9770s 16 9 splicing splice VERB 8910js9770s 16 10 products product NOUN 8910js9770s 16 11 were be AUX 8910js9770s 16 12 detected detect VERB 8910js9770s 16 13 both both PRON 8910js9770s 16 14 in in ADP 8910js9770s 16 15 vitro vitro X 8910js9770s 16 16 with with ADP 8910js9770s 16 17 t7 t7 PROPN 8910js9770s 16 18 rna rna NOUN 8910js9770s 16 19 transcripts transcript NOUN 8910js9770s 16 20 and and CCONJ 8910js9770s 16 21 in in ADP 8910js9770s 16 22 situ situ NOUN 8910js9770s 16 23 from from ADP 8910js9770s 16 24 traditional traditional ADJ 8910js9770s 16 25 rna rna PROPN 8910js9770s 16 26 polymerase polymerase PROPN 8910js9770s 16 27 ii ii PROPN 8910js9770s 16 28 ( ( PUNCT 8910js9770s 16 29 rnap rnap PROPN 8910js9770s 16 30 ii ii PROPN 8910js9770s 16 31 ) ) PUNCT 8910js9770s 16 32 promoted promote VERB 8910js9770s 16 33 transcripts transcript NOUN 8910js9770s 16 34 . . PUNCT 8910js9770s 17 1 a a DET 8910js9770s 17 2 number number NOUN 8910js9770s 17 3 of of ADP 8910js9770s 17 4 different different ADJ 8910js9770s 17 5 introns intron NOUN 8910js9770s 17 6 , , PUNCT 8910js9770s 17 7 which which PRON 8910js9770s 17 8 splice splice VERB 8910js9770s 17 9 with with ADP 8910js9770s 17 10 varying vary VERB 8910js9770s 17 11 efficiencies efficiency NOUN 8910js9770s 17 12 as as SCONJ 8910js9770s 17 13 measured measure VERB 8910js9770s 17 14 by by ADP 8910js9770s 17 15 real real ADJ 8910js9770s 17 16 - - PUNCT 8910js9770s 17 17 time time NOUN 8910js9770s 17 18 reverse reverse ADJ 8910js9770s 17 19 transcription transcription NOUN 8910js9770s 17 20 polymerase polymerase NOUN 8910js9770s 17 21 chain chain NOUN 8910js9770s 17 22 reaction reaction NOUN 8910js9770s 17 23 ( ( PUNCT 8910js9770s 17 24 pcr pcr NOUN 8910js9770s 17 25 ) ) PUNCT 8910js9770s 17 26 ( ( PUNCT 8910js9770s 17 27 qrt qrt ADJ 8910js9770s 17 28 - - PUNCT 8910js9770s 17 29 pcr pcr NOUN 8910js9770s 17 30 ) ) PUNCT 8910js9770s 17 31 and and CCONJ 8910js9770s 17 32 dual dual ADJ 8910js9770s 17 33 luciferase luciferase NOUN 8910js9770s 17 34 assay assay NOUN 8910js9770s 17 35 , , PUNCT 8910js9770s 17 36 have have AUX 8910js9770s 17 37 been be AUX 8910js9770s 17 38 designed design VERB 8910js9770s 17 39 and and CCONJ 8910js9770s 17 40 tested test VERB 8910js9770s 17 41 . . PUNCT 8910js9770s 18 1 the the DET 8910js9770s 18 2 trans trans NOUN 8910js9770s 18 3 - - NOUN 8910js9770s 18 4 splicing splicing NOUN 8910js9770s 18 5 of of ADP 8910js9770s 18 6 the the DET 8910js9770s 18 7 group group NOUN 8910js9770s 18 8 i i PRON 8910js9770s 18 9 intron intron X 8910js9770s 18 10 can can AUX 8910js9770s 18 11 be be AUX 8910js9770s 18 12 directed direct VERB 8910js9770s 18 13 to to PART 8910js9770s 18 14 splice splice VERB 8910js9770s 18 15 a a DET 8910js9770s 18 16 novel novel NOUN 8910js9770s 18 17 3 3 NUM 8910js9770s 18 18 ' ' NUM 8910js9770s 18 19 exon exon NOUN 8910js9770s 18 20 to to ADP 8910js9770s 18 21 the the DET 8910js9770s 18 22 upstream upstream ADJ 8910js9770s 18 23 fragment fragment NOUN 8910js9770s 18 24 of of ADP 8910js9770s 18 25 the the DET 8910js9770s 18 26 splicing splicing NOUN 8910js9770s 18 27 product product NOUN 8910js9770s 18 28 , , PUNCT 8910js9770s 18 29 allowing allow VERB 8910js9770s 18 30 for for ADP 8910js9770s 18 31 de de X 8910js9770s 18 32 novo novo X 8910js9770s 18 33 gene gene NOUN 8910js9770s 18 34 expression expression NOUN 8910js9770s 18 35 upon upon SCONJ 8910js9770s 18 36 detection detection NOUN 8910js9770s 18 37 of of ADP 8910js9770s 18 38 the the DET 8910js9770s 18 39 target target NOUN 8910js9770s 18 40 . . PUNCT 8910js9770s 19 1 when when SCONJ 8910js9770s 19 2 linked link VERB 8910js9770s 19 3 to to ADP 8910js9770s 19 4 a a DET 8910js9770s 19 5 pro pro ADJ 8910js9770s 19 6 - - ADJ 8910js9770s 19 7 apoptotic apoptotic ADJ 8910js9770s 19 8 gene gene NOUN 8910js9770s 19 9 as as ADP 8910js9770s 19 10 a a DET 8910js9770s 19 11 3 3 NUM 8910js9770s 19 12 ' ' NUM 8910js9770s 19 13 exon exon NOUN 8910js9770s 19 14 and and CCONJ 8910js9770s 19 15 expressed express VERB 8910js9770s 19 16 constitutively constitutively ADV 8910js9770s 19 17 in in ADP 8910js9770s 19 18 a a DET 8910js9770s 19 19 cell cell NOUN 8910js9770s 19 20 , , PUNCT 8910js9770s 19 21 the the DET 8910js9770s 19 22 group group NOUN 8910js9770s 19 23 i i PRON 8910js9770s 19 24 intron intron X 8910js9770s 19 25 may may AUX 8910js9770s 19 26 trigger trigger VERB 8910js9770s 19 27 ordered order VERB 8910js9770s 19 28 cell cell NOUN 8910js9770s 19 29 death death NOUN 8910js9770s 19 30 upon upon SCONJ 8910js9770s 19 31 infection infection NOUN 8910js9770s 19 32 , , PUNCT 8910js9770s 19 33 denying deny VERB 8910js9770s 19 34 the the DET 8910js9770s 19 35 virus virus NOUN 8910js9770s 19 36 the the DET 8910js9770s 19 37 ability ability NOUN 8910js9770s 19 38 to to PART 8910js9770s 19 39 reproduce reproduce VERB 8910js9770s 19 40 . . PUNCT 8910js9770s 20 1 one one NUM 8910js9770s 20 2 such such ADJ 8910js9770s 20 3 pro pro ADJ 8910js9770s 20 4 - - ADJ 8910js9770s 20 5 apoptotic apoptotic ADJ 8910js9770s 20 6 gene gene NOUN 8910js9770s 20 7 , , PUNCT 8910js9770s 20 8 the the DET 8910js9770s 20 9 truncated truncate VERB 8910js9770s 20 10 bcl-2 bcl-2 ADV 8910js9770s 20 11 - - PUNCT 8910js9770s 20 12 associated associate VERB 8910js9770s 20 13 protein protein NOUN 8910js9770s 20 14 x x SYM 8910js9770s 20 15 ( ( PUNCT 8910js9770s 20 16 tbax tbax PROPN 8910js9770s 20 17 ) ) PUNCT 8910js9770s 20 18 , , PUNCT 8910js9770s 20 19 was be AUX 8910js9770s 20 20 tested test VERB 8910js9770s 20 21 for for ADP 8910js9770s 20 22 its its PRON 8910js9770s 20 23 ability ability NOUN 8910js9770s 20 24 to to PART 8910js9770s 20 25 induce induce VERB 8910js9770s 20 26 apoptosis apoptosis NOUN 8910js9770s 20 27 in in ADP 8910js9770s 20 28 insect insect NOUN 8910js9770s 20 29 cells cell NOUN 8910js9770s 20 30 and and CCONJ 8910js9770s 20 31 found find VERB 8910js9770s 20 32 to to PART 8910js9770s 20 33 be be AUX 8910js9770s 20 34 an an DET 8910js9770s 20 35 effective effective ADJ 8910js9770s 20 36 pro pro ADJ 8910js9770s 20 37 - - ADJ 8910js9770s 20 38 apoptotic apoptotic ADJ 8910js9770s 20 39 gene gene NOUN 8910js9770s 20 40 . . PUNCT