id	sid	tid	token	lemma	pos
3b59183387t	1	1	autotransporter	autotransporter	PROPN
3b59183387t	1	2	(	(	PUNCT
3b59183387t	1	3	at	at	ADP
3b59183387t	1	4	)	)	PUNCT
3b59183387t	1	5	proteins	protein	NOUN
3b59183387t	1	6	are	be	AUX
3b59183387t	1	7	the	the	DET
3b59183387t	1	8	largest	large	ADJ
3b59183387t	1	9	family	family	NOUN
3b59183387t	1	10	of	of	ADP
3b59183387t	1	11	virulence	virulence	NOUN
3b59183387t	1	12	proteins	protein	NOUN
3b59183387t	1	13	secreted	secrete	VERB
3b59183387t	1	14	from	from	ADP
3b59183387t	1	15	gram	gram	ADJ
3b59183387t	1	16	-	-	PUNCT
3b59183387t	1	17	negative	negative	ADJ
3b59183387t	1	18	bacterial	bacterial	ADJ
3b59183387t	1	19	pathogens	pathogen	NOUN
3b59183387t	1	20	.	.	PUNCT
3b59183387t	2	1	they	they	PRON
3b59183387t	2	2	are	be	AUX
3b59183387t	2	3	synthesized	synthesize	VERB
3b59183387t	2	4	with	with	ADP
3b59183387t	2	5	an	an	DET
3b59183387t	2	6	n	n	ADJ
3b59183387t	2	7	-	-	PUNCT
3b59183387t	2	8	terminal	terminal	ADJ
3b59183387t	2	9	signal	signal	NOUN
3b59183387t	2	10	sequence	sequence	NOUN
3b59183387t	2	11	,	,	PUNCT
3b59183387t	2	12	a	a	DET
3b59183387t	2	13	central	central	ADJ
3b59183387t	2	14	passenger	passenger	NOUN
3b59183387t	2	15	domain	domain	NOUN
3b59183387t	2	16	(	(	PUNCT
3b59183387t	2	17	the	the	DET
3b59183387t	2	18	mature	mature	ADJ
3b59183387t	2	19	protein	protein	NOUN
3b59183387t	2	20	)	)	PUNCT
3b59183387t	2	21	,	,	PUNCT
3b59183387t	2	22	and	and	CCONJ
3b59183387t	2	23	a	a	DET
3b59183387t	2	24	c	c	X
3b59183387t	2	25	-	-	PUNCT
3b59183387t	2	26	terminal	terminal	ADJ
3b59183387t	2	27	outer	outer	ADJ
3b59183387t	2	28	membrane	membrane	NOUN
3b59183387t	2	29	(	(	PUNCT
3b59183387t	2	30	om	om	NOUN
3b59183387t	2	31	)	)	PUNCT
3b59183387t	2	32	translocator	translocator	NOUN
3b59183387t	2	33	domain	domain	NOUN
3b59183387t	2	34	.	.	PUNCT
3b59183387t	3	1	the	the	DET
3b59183387t	3	2	translocator	translocator	NOUN
3b59183387t	3	3	domain	domain	NOUN
3b59183387t	3	4	is	be	AUX
3b59183387t	3	5	required	require	VERB
3b59183387t	3	6	for	for	ADP
3b59183387t	3	7	om	om	PROPN
3b59183387t	3	8	secretion	secretion	NOUN
3b59183387t	3	9	of	of	ADP
3b59183387t	3	10	the	the	DET
3b59183387t	3	11	central	central	ADJ
3b59183387t	3	12	passenger	passenger	NOUN
3b59183387t	3	13	domain	domain	NOUN
3b59183387t	3	14	.	.	PUNCT
3b59183387t	4	1	transport	transport	NOUN
3b59183387t	4	2	of	of	ADP
3b59183387t	4	3	the	the	DET
3b59183387t	4	4	passenger	passenger	NOUN
3b59183387t	4	5	domain	domain	NOUN
3b59183387t	4	6	across	across	ADP
3b59183387t	4	7	the	the	DET
3b59183387t	4	8	om	om	NOUN
3b59183387t	4	9	does	do	AUX
3b59183387t	4	10	not	not	PART
3b59183387t	4	11	require	require	VERB
3b59183387t	4	12	atp	atp	PROPN
3b59183387t	4	13	nor	nor	CCONJ
3b59183387t	4	14	a	a	DET
3b59183387t	4	15	proton	proton	NOUN
3b59183387t	4	16	gradient	gradient	NOUN
3b59183387t	4	17	,	,	PUNCT
3b59183387t	4	18	and	and	CCONJ
3b59183387t	4	19	therefore	therefore	ADV
3b59183387t	4	20	the	the	DET
3b59183387t	4	21	driving	drive	VERB
3b59183387t	4	22	force	force	NOUN
3b59183387t	4	23	for	for	ADP
3b59183387t	4	24	efficient	efficient	ADJ
3b59183387t	4	25	secretion	secretion	NOUN
3b59183387t	4	26	remains	remain	VERB
3b59183387t	4	27	unknown	unknown	ADJ
3b59183387t	4	28	.	.	PUNCT
3b59183387t	5	1	previous	previous	ADJ
3b59183387t	5	2	studies	study	NOUN
3b59183387t	5	3	indicate	indicate	VERB
3b59183387t	5	4	that	that	SCONJ
3b59183387t	5	5	the	the	PRON
3b59183387t	5	6	at	at	ADP
3b59183387t	5	7	passenger	passenger	NOUN
3b59183387t	5	8	domain	domain	NOUN
3b59183387t	5	9	is	be	AUX
3b59183387t	5	10	secreted	secrete	VERB
3b59183387t	5	11	from	from	ADP
3b59183387t	5	12	cto	cto	PROPN
3b59183387t	5	13	n	n	NOUN
3b59183387t	5	14	-	-	NOUN
3b59183387t	5	15	terminus	terminus	NOUN
3b59183387t	5	16	across	across	ADP
3b59183387t	5	17	the	the	DET
3b59183387t	5	18	om	om	NOUN
3b59183387t	5	19	,	,	PUNCT
3b59183387t	5	20	and	and	CCONJ
3b59183387t	5	21	that	that	SCONJ
3b59183387t	5	22	,	,	PUNCT
3b59183387t	5	23	once	once	ADV
3b59183387t	5	24	outside	outside	ADP
3b59183387t	5	25	of	of	ADP
3b59183387t	5	26	the	the	DET
3b59183387t	5	27	cell	cell	NOUN
3b59183387t	5	28	,	,	PUNCT
3b59183387t	5	29	vectorial	vectorial	ADJ
3b59183387t	5	30	folding	folding	NOUN
3b59183387t	5	31	of	of	ADP
3b59183387t	5	32	the	the	DET
3b59183387t	5	33	passenger	passenger	NOUN
3b59183387t	5	34	could	could	AUX
3b59183387t	5	35	drive	drive	VERB
3b59183387t	5	36	om	om	PROPN
3b59183387t	5	37	secretion	secretion	NOUN
3b59183387t	5	38	.	.	PUNCT
3b59183387t	6	1	this	this	DET
3b59183387t	6	2	mechanism	mechanism	NOUN
3b59183387t	6	3	implies	imply	VERB
3b59183387t	6	4	that	that	SCONJ
3b59183387t	6	5	the	the	PRON
3b59183387t	6	6	at	at	ADP
3b59183387t	6	7	passenger	passenger	NOUN
3b59183387t	6	8	domain	domain	NOUN
3b59183387t	6	9	must	must	AUX
3b59183387t	6	10	remain	remain	VERB
3b59183387t	6	11	in	in	ADP
3b59183387t	6	12	an	an	DET
3b59183387t	6	13	unfolded	unfolded	ADJ
3b59183387t	6	14	,	,	PUNCT
3b59183387t	6	15	secretion	secretion	NOUN
3b59183387t	6	16	-	-	PUNCT
3b59183387t	6	17	competent	competent	ADJ
3b59183387t	6	18	conformation	conformation	NOUN
3b59183387t	6	19	in	in	ADP
3b59183387t	6	20	the	the	DET
3b59183387t	6	21	periplasm	periplasm	NOUN
3b59183387t	6	22	,	,	PUNCT
3b59183387t	6	23	and	and	CCONJ
3b59183387t	6	24	that	that	SCONJ
3b59183387t	6	25	disrupting	disrupt	VERB
3b59183387t	6	26	the	the	DET
3b59183387t	6	27	vectorial	vectorial	ADJ
3b59183387t	6	28	folding	fold	VERB
3b59183387t	6	29	process	process	NOUN
3b59183387t	6	30	across	across	ADP
3b59183387t	6	31	the	the	DET
3b59183387t	6	32	om	om	PROPN
3b59183387t	6	33	would	would	AUX
3b59183387t	6	34	hinder	hinder	VERB
3b59183387t	6	35	secretion	secretion	NOUN
3b59183387t	6	36	.	.	PUNCT
3b59183387t	7	1	to	to	PART
3b59183387t	7	2	test	test	VERB
3b59183387t	7	3	this	this	DET
3b59183387t	7	4	model	model	NOUN
3b59183387t	7	5	,	,	PUNCT
3b59183387t	7	6	i	i	PRON
3b59183387t	7	7	developed	develop	VERB
3b59183387t	7	8	a	a	DET
3b59183387t	7	9	combination	combination	NOUN
3b59183387t	7	10	of	of	ADP
3b59183387t	7	11	biochemical	biochemical	ADJ
3b59183387t	7	12	and	and	CCONJ
3b59183387t	7	13	biophysical	biophysical	ADJ
3b59183387t	7	14	approaches	approach	NOUN
3b59183387t	7	15	,	,	PUNCT
3b59183387t	7	16	using	use	VERB
3b59183387t	7	17	the	the	DET
3b59183387t	7	18	model	model	NOUN
3b59183387t	7	19	at	at	ADP
3b59183387t	7	20	pertactin	pertactin	NOUN
3b59183387t	7	21	from	from	ADP
3b59183387t	7	22	b.	b.	PROPN
3b59183387t	7	23	pertussis	pertussis	PROPN
3b59183387t	7	24	.	.	PUNCT
3b59183387t	8	1	using	use	VERB
3b59183387t	8	2	a	a	DET
3b59183387t	8	3	novel	novel	NOUN
3b59183387t	8	4	in	in	ADP
3b59183387t	8	5	vivo	vivo	ADJ
3b59183387t	8	6	antibiotic	antibiotic	ADJ
3b59183387t	8	7	-	-	PUNCT
3b59183387t	8	8	resistance	resistance	NOUN
3b59183387t	8	9	based	base	VERB
3b59183387t	8	10	assay	assay	PROPN
3b59183387t	8	11	,	,	PUNCT
3b59183387t	8	12	i	i	PRON
3b59183387t	8	13	was	be	AUX
3b59183387t	8	14	able	able	ADJ
3b59183387t	8	15	to	to	PART
3b59183387t	8	16	demonstrate	demonstrate	VERB
3b59183387t	8	17	that	that	SCONJ
3b59183387t	8	18	pertactin	pertactin	NOUN
3b59183387t	8	19	adopts	adopt	VERB
3b59183387t	8	20	a	a	DET
3b59183387t	8	21	non	non	ADJ
3b59183387t	8	22	-	-	ADJ
3b59183387t	8	23	native	native	ADJ
3b59183387t	8	24	,	,	PUNCT
3b59183387t	8	25	protease	protease	NOUN
3b59183387t	8	26	susceptible	susceptible	ADJ
3b59183387t	8	27	conformation	conformation	NOUN
3b59183387t	8	28	in	in	ADP
3b59183387t	8	29	the	the	DET
3b59183387t	8	30	periplasm	periplasm	NOUN
3b59183387t	8	31	,	,	PUNCT
3b59183387t	8	32	confirming	confirm	VERB
3b59183387t	8	33	that	that	SCONJ
3b59183387t	8	34	this	this	DET
3b59183387t	8	35	requirement	requirement	NOUN
3b59183387t	8	36	for	for	ADP
3b59183387t	8	37	vectorial	vectorial	ADJ
3b59183387t	8	38	secretion	secretion	NOUN
3b59183387t	8	39	through	through	ADP
3b59183387t	8	40	its	its	PRON
3b59183387t	8	41	own	own	ADJ
3b59183387t	8	42	translocator	translocator	NOUN
3b59183387t	8	43	domain	domain	NOUN
3b59183387t	8	44	is	be	AUX
3b59183387t	8	45	met	meet	VERB
3b59183387t	8	46	.	.	PUNCT
3b59183387t	9	1	to	to	PART
3b59183387t	9	2	gain	gain	VERB
3b59183387t	9	3	a	a	DET
3b59183387t	9	4	better	well	ADJ
3b59183387t	9	5	understanding	understanding	NOUN
3b59183387t	9	6	of	of	ADP
3b59183387t	9	7	the	the	DET
3b59183387t	9	8	om	om	PROPN
3b59183387t	9	9	secretion	secretion	NOUN
3b59183387t	9	10	process	process	NOUN
3b59183387t	9	11	,	,	PUNCT
3b59183387t	9	12	i	i	PRON
3b59183387t	9	13	developed	develop	VERB
3b59183387t	9	14	an	an	DET
3b59183387t	9	15	in	in	ADP
3b59183387t	9	16	vitro	vitro	X
3b59183387t	9	17	vesicle	vesicle	NOUN
3b59183387t	9	18	-	-	PUNCT
3b59183387t	9	19	based	base	VERB
3b59183387t	9	20	secretion	secretion	NOUN
3b59183387t	9	21	system	system	NOUN
3b59183387t	9	22	.	.	PUNCT
3b59183387t	10	1	the	the	DET
3b59183387t	10	2	purified	purify	VERB
3b59183387t	10	3	passenger+translocator	passenger+translocator	NOUN
3b59183387t	10	4	construct	construct	NOUN
3b59183387t	10	5	was	be	AUX
3b59183387t	10	6	successfully	successfully	ADV
3b59183387t	10	7	refolded	refolde	VERB
3b59183387t	10	8	in	in	ADP
3b59183387t	10	9	a	a	DET
3b59183387t	10	10	lipid	lipid	NOUN
3b59183387t	10	11	environment	environment	NOUN
3b59183387t	10	12	,	,	PUNCT
3b59183387t	10	13	followed	follow	VERB
3b59183387t	10	14	by	by	ADP
3b59183387t	10	15	autocatalytic	autocatalytic	ADJ
3b59183387t	10	16	cleavage	cleavage	NOUN
3b59183387t	10	17	of	of	ADP
3b59183387t	10	18	the	the	DET
3b59183387t	10	19	translocator	translocator	NOUN
3b59183387t	10	20	from	from	ADP
3b59183387t	10	21	its	its	PRON
3b59183387t	10	22	passenger	passenger	NOUN
3b59183387t	10	23	domain	domain	NOUN
3b59183387t	10	24	.	.	PUNCT
3b59183387t	11	1	ats	at	NOUN
3b59183387t	11	2	are	be	AUX
3b59183387t	11	3	unique	unique	ADJ
3b59183387t	11	4	folding	fold	VERB
3b59183387t	11	5	model	model	NOUN
3b59183387t	11	6	proteins	protein	NOUN
3b59183387t	11	7	,	,	PUNCT
3b59183387t	11	8	because	because	SCONJ
3b59183387t	11	9	it	it	PRON
3b59183387t	11	10	was	be	AUX
3b59183387t	11	11	proposed	propose	VERB
3b59183387t	11	12	that	that	SCONJ
3b59183387t	11	13	they	they	PRON
3b59183387t	11	14	fold	fold	VERB
3b59183387t	11	15	in	in	ADP
3b59183387t	11	16	a	a	DET
3b59183387t	11	17	vectorial	vectorial	ADJ
3b59183387t	11	18	manner	manner	NOUN
3b59183387t	11	19	upon	upon	SCONJ
3b59183387t	11	20	om	om	PROPN
3b59183387t	11	21	secretion	secretion	NOUN
3b59183387t	11	22	.	.	PUNCT
3b59183387t	12	1	this	this	PRON
3b59183387t	12	2	is	be	AUX
3b59183387t	12	3	an	an	DET
3b59183387t	12	4	intriguing	intriguing	ADJ
3b59183387t	12	5	hypothesis	hypothesis	NOUN
3b59183387t	12	6	,	,	PUNCT
3b59183387t	12	7	but	but	CCONJ
3b59183387t	12	8	it	it	PRON
3b59183387t	12	9	was	be	AUX
3b59183387t	12	10	previously	previously	ADV
3b59183387t	12	11	impossible	impossible	ADJ
3b59183387t	12	12	to	to	PART
3b59183387t	12	13	test	test	VERB
3b59183387t	12	14	this	this	DET
3b59183387t	12	15	hypothesis	hypothesis	NOUN
3b59183387t	12	16	directly	directly	ADV
3b59183387t	12	17	.	.	PUNCT
3b59183387t	13	1	therefore	therefore	ADV
3b59183387t	13	2	,	,	PUNCT
3b59183387t	13	3	i	i	PRON
3b59183387t	13	4	developed	develop	VERB
3b59183387t	13	5	the	the	DET
3b59183387t	13	6	foundations	foundation	NOUN
3b59183387t	13	7	of	of	ADP
3b59183387t	13	8	a	a	DET
3b59183387t	13	9	single	single	ADJ
3b59183387t	13	10	molecule	molecule	NOUN
3b59183387t	13	11	system	system	NOUN
3b59183387t	13	12	to	to	PART
3b59183387t	13	13	measure	measure	VERB
3b59183387t	13	14	vectorial	vectorial	ADJ
3b59183387t	13	15	folding	folding	NOUN
3b59183387t	13	16	of	of	ADP
3b59183387t	13	17	ats	at	NOUN
3b59183387t	13	18	in	in	ADP
3b59183387t	13	19	a	a	DET
3b59183387t	13	20	controlled	control	VERB
3b59183387t	13	21	in	in	ADP
3b59183387t	13	22	vitro	vitro	X
3b59183387t	13	23	system	system	NOUN
3b59183387t	13	24	directly	directly	ADV
3b59183387t	13	25	.	.	PUNCT
3b59183387t	14	1	this	this	DET
3b59183387t	14	2	novel	novel	NOUN
3b59183387t	14	3	setup	setup	NOUN
3b59183387t	14	4	includes	include	VERB
3b59183387t	14	5	nanopore	nanopore	ADJ
3b59183387t	14	6	translocation	translocation	NOUN
3b59183387t	14	7	and	and	CCONJ
3b59183387t	14	8	subsequent	subsequent	ADJ
3b59183387t	14	9	detection	detection	NOUN
3b59183387t	14	10	of	of	ADP
3b59183387t	14	11	protein	protein	NOUN
3b59183387t	14	12	folding	fold	VERB
3b59183387t	14	13	using	use	VERB
3b59183387t	14	14	a	a	DET
3b59183387t	14	15	single	single	ADJ
3b59183387t	14	16	molecule	molecule	NOUN
3b59183387t	14	17	fluorescence	fluorescence	NOUN
3b59183387t	14	18	based	base	VERB
3b59183387t	14	19	assay	assay	PROPN
3b59183387t	14	20	.	.	PUNCT
3b59183387t	15	1	here	here	ADV
3b59183387t	15	2	,	,	PUNCT
3b59183387t	15	3	both	both	DET
3b59183387t	15	4	parts	part	NOUN
3b59183387t	15	5	of	of	ADP
3b59183387t	15	6	this	this	DET
3b59183387t	15	7	system	system	NOUN
3b59183387t	15	8	were	be	AUX
3b59183387t	15	9	implemented	implement	VERB
3b59183387t	15	10	separately	separately	ADV
3b59183387t	15	11	as	as	ADP
3b59183387t	15	12	proof	proof	NOUN
3b59183387t	15	13	of	of	ADP
3b59183387t	15	14	principle	principle	ADJ
3b59183387t	15	15	tests	test	NOUN
3b59183387t	15	16	of	of	ADP
3b59183387t	15	17	feasibility	feasibility	NOUN
3b59183387t	15	18	.	.	PUNCT
3b59183387t	16	1	taken	take	VERB
3b59183387t	16	2	together	together	ADV
3b59183387t	16	3	,	,	PUNCT
3b59183387t	16	4	these	these	DET
3b59183387t	16	5	studies	study	NOUN
3b59183387t	16	6	provide	provide	VERB
3b59183387t	16	7	a	a	DET
3b59183387t	16	8	unique	unique	ADJ
3b59183387t	16	9	perspective	perspective	NOUN
3b59183387t	16	10	to	to	PART
3b59183387t	16	11	get	get	VERB
3b59183387t	16	12	insights	insight	NOUN
3b59183387t	16	13	in	in	ADP
3b59183387t	16	14	a	a	DET
3b59183387t	16	15	complex	complex	ADJ
3b59183387t	16	16	protein	protein	NOUN
3b59183387t	16	17	folding	fold	VERB
3b59183387t	16	18	process	process	NOUN
3b59183387t	16	19	.	.	PUNCT