id sid tid token lemma pos 0k225b01f90 1 1 the the DET 0k225b01f90 1 2 motor motor NOUN 0k225b01f90 1 3 protein protein NOUN 0k225b01f90 1 4 cytoplasmic cytoplasmic ADJ 0k225b01f90 1 5 dynein dynein PROPN 0k225b01f90 1 6 has have AUX 0k225b01f90 1 7 been be AUX 0k225b01f90 1 8 implicated implicate VERB 0k225b01f90 1 9 in in ADP 0k225b01f90 1 10 mitotic mitotic ADJ 0k225b01f90 1 11 functions function NOUN 0k225b01f90 1 12 that that PRON 0k225b01f90 1 13 require require VERB 0k225b01f90 1 14 dynein dynein NOUN 0k225b01f90 1 15 localization localization NOUN 0k225b01f90 1 16 to to ADP 0k225b01f90 1 17 specific specific ADJ 0k225b01f90 1 18 cellular cellular ADJ 0k225b01f90 1 19 locations location NOUN 0k225b01f90 1 20 . . PUNCT 0k225b01f90 2 1 recent recent ADJ 0k225b01f90 2 2 studies study NOUN 0k225b01f90 2 3 in in ADP 0k225b01f90 2 4 our our PRON 0k225b01f90 2 5 lab lab NOUN 0k225b01f90 2 6 have have AUX 0k225b01f90 2 7 focused focus VERB 0k225b01f90 2 8 on on ADP 0k225b01f90 2 9 the the DET 0k225b01f90 2 10 hypothesis hypothesis NOUN 0k225b01f90 2 11 that that SCONJ 0k225b01f90 2 12 phosphorylation phosphorylation NOUN 0k225b01f90 2 13 events event NOUN 0k225b01f90 2 14 determine determine VERB 0k225b01f90 2 15 the the DET 0k225b01f90 2 16 targeting targeting NOUN 0k225b01f90 2 17 of of ADP 0k225b01f90 2 18 dynein dynein NOUN 0k225b01f90 2 19 to to ADP 0k225b01f90 2 20 kinetochores kinetochore NOUN 0k225b01f90 2 21 and and CCONJ 0k225b01f90 2 22 spindle spindle NOUN 0k225b01f90 2 23 poles pole NOUN 0k225b01f90 2 24 . . PUNCT 0k225b01f90 3 1 we we PRON 0k225b01f90 3 2 previously previously ADV 0k225b01f90 3 3 identified identify VERB 0k225b01f90 3 4 a a DET 0k225b01f90 3 5 novel novel ADJ 0k225b01f90 3 6 phosphorylation phosphorylation NOUN 0k225b01f90 3 7 site site NOUN 0k225b01f90 3 8 at at ADP 0k225b01f90 3 9 threonine threonine NOUN 0k225b01f90 3 10 89 89 NUM 0k225b01f90 3 11 ( ( PUNCT 0k225b01f90 3 12 t89 t89 PROPN 0k225b01f90 3 13 ) ) PUNCT 0k225b01f90 3 14 of of ADP 0k225b01f90 3 15 the the DET 0k225b01f90 3 16 dynein dynein PROPN 0k225b01f90 3 17 intermediate intermediate ADJ 0k225b01f90 3 18 chain chain NOUN 0k225b01f90 3 19 that that PRON 0k225b01f90 3 20 targets target VERB 0k225b01f90 3 21 dynein dynein NOUN 0k225b01f90 3 22 to to ADP 0k225b01f90 3 23 the the DET 0k225b01f90 3 24 kinetochore kinetochore NOUN 0k225b01f90 3 25 during during ADP 0k225b01f90 3 26 mitosis mitosis NOUN 0k225b01f90 3 27 ( ( PUNCT 0k225b01f90 3 28 whyte whyte NOUN 0k225b01f90 3 29 et et PROPN 0k225b01f90 3 30 al al PROPN 0k225b01f90 3 31 . . PROPN 0k225b01f90 3 32 , , PUNCT 0k225b01f90 3 33 2008 2008 NUM 0k225b01f90 3 34 ) ) PUNCT 0k225b01f90 3 35 . . PUNCT 0k225b01f90 4 1 in in ADP 0k225b01f90 4 2 this this DET 0k225b01f90 4 3 study study NOUN 0k225b01f90 4 4 , , PUNCT 0k225b01f90 4 5 we we PRON 0k225b01f90 4 6 analyzed analyze VERB 0k225b01f90 4 7 a a DET 0k225b01f90 4 8 second second ADJ 0k225b01f90 4 9 novel novel NOUN 0k225b01f90 4 10 dynein dynein NOUN 0k225b01f90 4 11 phosphorylation phosphorylation NOUN 0k225b01f90 4 12 site site NOUN 0k225b01f90 4 13 at at ADP 0k225b01f90 4 14 tyrosine tyrosine NOUN 0k225b01f90 4 15 130 130 NUM 0k225b01f90 4 16 ( ( PUNCT 0k225b01f90 4 17 y130 y130 PROPN 0k225b01f90 4 18 ) ) PUNCT 0k225b01f90 4 19 that that PRON 0k225b01f90 4 20 results result VERB 0k225b01f90 4 21 in in ADP 0k225b01f90 4 22 dynein dynein NOUN 0k225b01f90 4 23 localization localization NOUN 0k225b01f90 4 24 to to PART 0k225b01f90 4 25 spindle spindle NOUN 0k225b01f90 4 26 poles pole NOUN 0k225b01f90 4 27 . . PUNCT 0k225b01f90 5 1 we we PRON 0k225b01f90 5 2 also also ADV 0k225b01f90 5 3 examined examine VERB 0k225b01f90 5 4 the the DET 0k225b01f90 5 5 contributions contribution NOUN 0k225b01f90 5 6 of of ADP 0k225b01f90 5 7 the the DET 0k225b01f90 5 8 spindle spindle NOUN 0k225b01f90 5 9 assembly assembly NOUN 0k225b01f90 5 10 checkpoint checkpoint NOUN 0k225b01f90 5 11 ( ( PUNCT 0k225b01f90 5 12 sac sac NOUN 0k225b01f90 5 13 ) ) PUNCT 0k225b01f90 5 14 protein protein NOUN 0k225b01f90 5 15 spindly spindly ADV 0k225b01f90 5 16 to to ADP 0k225b01f90 5 17 regulation regulation NOUN 0k225b01f90 5 18 of of ADP 0k225b01f90 5 19 dynein dynein PROPN 0k225b01f90 5 20 kinetochore kinetochore NOUN 0k225b01f90 5 21 transitions transition NOUN 0k225b01f90 5 22 . . PUNCT 0k225b01f90 6 1 preliminary preliminary ADJ 0k225b01f90 6 2 experiments experiment NOUN 0k225b01f90 6 3 defined define VERB 0k225b01f90 6 4 py130 py130 NOUN 0k225b01f90 6 5 - - PUNCT 0k225b01f90 6 6 dynein dynein NOUN 0k225b01f90 6 7 as as ADV 0k225b01f90 6 8 separate separate ADJ 0k225b01f90 6 9 from from ADP 0k225b01f90 6 10 the the DET 0k225b01f90 6 11 kinetochore kinetochore NOUN 0k225b01f90 6 12 dynein dynein PROPN 0k225b01f90 6 13 that that PRON 0k225b01f90 6 14 functions function NOUN 0k225b01f90 6 15 as as ADP 0k225b01f90 6 16 part part NOUN 0k225b01f90 6 17 of of ADP 0k225b01f90 6 18 the the DET 0k225b01f90 6 19 spindle spindle NOUN 0k225b01f90 6 20 assembly assembly NOUN 0k225b01f90 6 21 checkpoint checkpoint NOUN 0k225b01f90 6 22 . . PUNCT 0k225b01f90 7 1 to to PART 0k225b01f90 7 2 define define VERB 0k225b01f90 7 3 the the DET 0k225b01f90 7 4 requirements requirement NOUN 0k225b01f90 7 5 for for ADP 0k225b01f90 7 6 py130 py130 ADJ 0k225b01f90 7 7 - - PUNCT 0k225b01f90 7 8 dynein dynein NOUN 0k225b01f90 7 9 targeting targeting NOUN 0k225b01f90 7 10 to to ADP 0k225b01f90 7 11 spindle spindle NOUN 0k225b01f90 7 12 poles pole NOUN 0k225b01f90 7 13 , , PUNCT 0k225b01f90 7 14 we we PRON 0k225b01f90 7 15 disrupted disrupt VERB 0k225b01f90 7 16 candidate candidate NOUN 0k225b01f90 7 17 dynein dynein NOUN 0k225b01f90 7 18 binding bind VERB 0k225b01f90 7 19 proteins protein NOUN 0k225b01f90 7 20 and and CCONJ 0k225b01f90 7 21 assessed assess VERB 0k225b01f90 7 22 localization localization NOUN 0k225b01f90 7 23 of of ADP 0k225b01f90 7 24 py130 py130 NOUN 0k225b01f90 7 25 - - PUNCT 0k225b01f90 7 26 dynein dynein NOUN 0k225b01f90 7 27 . . PUNCT 0k225b01f90 8 1 dominant dominant PROPN 0k225b01f90 8 2 negative negative ADJ 0k225b01f90 8 3 fak fak PROPN 0k225b01f90 8 4 , , PUNCT 0k225b01f90 8 5 frnk frnk ADJ 0k225b01f90 8 6 , , PUNCT 0k225b01f90 8 7 asap asap PROPN 0k225b01f90 8 8 , , PUNCT 0k225b01f90 8 9 nudel nudel NOUN 0k225b01f90 8 10 and and CCONJ 0k225b01f90 8 11 cenpf cenpf NOUN 0k225b01f90 8 12 constructs construct NOUN 0k225b01f90 8 13 induced induce VERB 0k225b01f90 8 14 multipolar multipolar ADJ 0k225b01f90 8 15 and and CCONJ 0k225b01f90 8 16 fragmented fragmented ADJ 0k225b01f90 8 17 spindle spindle NOUN 0k225b01f90 8 18 poles pole NOUN 0k225b01f90 8 19 , , PUNCT 0k225b01f90 8 20 but but CCONJ 0k225b01f90 8 21 py130 py130 ADJ 0k225b01f90 8 22 - - PUNCT 0k225b01f90 8 23 dynein dynein NOUN 0k225b01f90 8 24 targeting targeting NOUN 0k225b01f90 8 25 to to ADP 0k225b01f90 8 26 spindle spindle NOUN 0k225b01f90 8 27 poles pole NOUN 0k225b01f90 8 28 was be AUX 0k225b01f90 8 29 unaffected unaffected ADJ 0k225b01f90 8 30 . . PUNCT 0k225b01f90 9 1 these these DET 0k225b01f90 9 2 results result NOUN 0k225b01f90 9 3 suggest suggest VERB 0k225b01f90 9 4 a a DET 0k225b01f90 9 5 model model NOUN 0k225b01f90 9 6 in in ADP 0k225b01f90 9 7 which which DET 0k225b01f90 9 8 specific specific ADJ 0k225b01f90 9 9 proteins protein NOUN 0k225b01f90 9 10 at at ADP 0k225b01f90 9 11 spindle spindle NOUN 0k225b01f90 9 12 poles pole NOUN 0k225b01f90 9 13 are be AUX 0k225b01f90 9 14 necessary necessary ADJ 0k225b01f90 9 15 for for ADP 0k225b01f90 9 16 dynein dynein NOUN 0k225b01f90 9 17 function function NOUN 0k225b01f90 9 18 , , PUNCT 0k225b01f90 9 19 whereas whereas SCONJ 0k225b01f90 9 20 other other ADJ 0k225b01f90 9 21 spindle spindle NOUN 0k225b01f90 9 22 pole pole NOUN 0k225b01f90 9 23 proteins protein NOUN 0k225b01f90 9 24 are be AUX 0k225b01f90 9 25 required require VERB 0k225b01f90 9 26 for for ADP 0k225b01f90 9 27 dynein dynein NOUN 0k225b01f90 9 28 recruitment recruitment NOUN 0k225b01f90 9 29 . . PUNCT 0k225b01f90 10 1 mass mass NOUN 0k225b01f90 10 2 spectrometry spectrometry NOUN 0k225b01f90 10 3 analysis analysis NOUN 0k225b01f90 10 4 of of ADP 0k225b01f90 10 5 isolated isolated ADJ 0k225b01f90 10 6 centrosomes centrosome NOUN 0k225b01f90 10 7 confirmed confirm VERB 0k225b01f90 10 8 the the DET 0k225b01f90 10 9 presence presence NOUN 0k225b01f90 10 10 of of ADP 0k225b01f90 10 11 py130 py130 NOUN 0k225b01f90 10 12 - - PUNCT 0k225b01f90 10 13 dynein dynein NOUN 0k225b01f90 10 14 at at ADP 0k225b01f90 10 15 centrosomes centrosome NOUN 0k225b01f90 10 16 and and CCONJ 0k225b01f90 10 17 suggests suggest VERB 0k225b01f90 10 18 that that SCONJ 0k225b01f90 10 19 py130 py130 ADJ 0k225b01f90 10 20 - - PUNCT 0k225b01f90 10 21 dynein dynein NOUN 0k225b01f90 10 22 associates associate NOUN 0k225b01f90 10 23 with with ADP 0k225b01f90 10 24 the the DET 0k225b01f90 10 25 inner inner ADJ 0k225b01f90 10 26 centrosome centrosome NOUN 0k225b01f90 10 27 . . PUNCT 0k225b01f90 11 1 altogether altogether ADV 0k225b01f90 11 2 , , PUNCT 0k225b01f90 11 3 these these DET 0k225b01f90 11 4 results result NOUN 0k225b01f90 11 5 provide provide VERB 0k225b01f90 11 6 insight insight NOUN 0k225b01f90 11 7 into into ADP 0k225b01f90 11 8 the the DET 0k225b01f90 11 9 potential potential ADJ 0k225b01f90 11 10 interactions interaction NOUN 0k225b01f90 11 11 of of ADP 0k225b01f90 11 12 py130 py130 NOUN 0k225b01f90 11 13 - - PUNCT 0k225b01f90 11 14 dynein dynein NOUN 0k225b01f90 11 15 at at ADP 0k225b01f90 11 16 spindle spindle NOUN 0k225b01f90 11 17 poles pole NOUN 0k225b01f90 11 18 . . PUNCT 0k225b01f90 12 1 spindly spindly ADJ 0k225b01f90 12 2 is be AUX 0k225b01f90 12 3 a a DET 0k225b01f90 12 4 novel novel ADJ 0k225b01f90 12 5 spindle spindle NOUN 0k225b01f90 12 6 assembly assembly NOUN 0k225b01f90 12 7 checkpoint checkpoint NOUN 0k225b01f90 12 8 protein protein NOUN 0k225b01f90 12 9 and and CCONJ 0k225b01f90 12 10 that that PRON 0k225b01f90 12 11 has have AUX 0k225b01f90 12 12 been be AUX 0k225b01f90 12 13 recently recently ADV 0k225b01f90 12 14 implicated implicate VERB 0k225b01f90 12 15 recruitment recruitment NOUN 0k225b01f90 12 16 of of ADP 0k225b01f90 12 17 dynein dynein NOUN 0k225b01f90 12 18 to to ADP 0k225b01f90 12 19 kinetochores kinetochore NOUN 0k225b01f90 12 20 ; ; PUNCT 0k225b01f90 12 21 however however ADV 0k225b01f90 12 22 , , PUNCT 0k225b01f90 12 23 the the DET 0k225b01f90 12 24 specific specific ADJ 0k225b01f90 12 25 contributions contribution NOUN 0k225b01f90 12 26 of of ADP 0k225b01f90 12 27 spindly spindly ADJ 0k225b01f90 12 28 to to PART 0k225b01f90 12 29 dynein dynein VERB 0k225b01f90 12 30 regulation regulation NOUN 0k225b01f90 12 31 and and CCONJ 0k225b01f90 12 32 silencing silencing NOUN 0k225b01f90 12 33 of of ADP 0k225b01f90 12 34 the the DET 0k225b01f90 12 35 spindle spindle NOUN 0k225b01f90 12 36 assembly assembly NOUN 0k225b01f90 12 37 checkpoint checkpoint NOUN 0k225b01f90 12 38 ( ( PUNCT 0k225b01f90 12 39 sac sac NOUN 0k225b01f90 12 40 ) ) PUNCT 0k225b01f90 12 41 are be AUX 0k225b01f90 12 42 unclear unclear ADJ 0k225b01f90 12 43 . . PUNCT 0k225b01f90 13 1 in in ADP 0k225b01f90 13 2 this this DET 0k225b01f90 13 3 study study NOUN 0k225b01f90 13 4 , , PUNCT 0k225b01f90 13 5 the the DET 0k225b01f90 13 6 effects effect NOUN 0k225b01f90 13 7 of of ADP 0k225b01f90 13 8 spindly spindly ADV 0k225b01f90 13 9 depletion depletion NOUN 0k225b01f90 13 10 on on ADP 0k225b01f90 13 11 different different ADJ 0k225b01f90 13 12 populations population NOUN 0k225b01f90 13 13 of of ADP 0k225b01f90 13 14 kinetochore kinetochore PROPN 0k225b01f90 13 15 dynein dynein PROPN 0k225b01f90 13 16 , , PUNCT 0k225b01f90 13 17 and and CCONJ 0k225b01f90 13 18 dynein dynein NOUN 0k225b01f90 13 19 poleward poleward ADJ 0k225b01f90 13 20 motility motility NOUN 0k225b01f90 13 21 were be AUX 0k225b01f90 13 22 measured measure VERB 0k225b01f90 13 23 . . PUNCT 0k225b01f90 14 1 together together ADV 0k225b01f90 14 2 with with ADP 0k225b01f90 14 3 previous previous ADJ 0k225b01f90 14 4 studies study NOUN 0k225b01f90 14 5 demonstrating demonstrate VERB 0k225b01f90 14 6 the the DET 0k225b01f90 14 7 ability ability NOUN 0k225b01f90 14 8 of of ADP 0k225b01f90 14 9 dynein dynein NOUN 0k225b01f90 14 10 to to PART 0k225b01f90 14 11 translocate translocate VERB 0k225b01f90 14 12 to to PART 0k225b01f90 14 13 spindle spindle VERB 0k225b01f90 14 14 poles pole NOUN 0k225b01f90 14 15 in in ADP 0k225b01f90 14 16 the the DET 0k225b01f90 14 17 absence absence NOUN 0k225b01f90 14 18 of of ADP 0k225b01f90 14 19 dynactin dynactin NOUN 0k225b01f90 14 20 , , PUNCT 0k225b01f90 14 21 these these DET 0k225b01f90 14 22 results result NOUN 0k225b01f90 14 23 of of ADP 0k225b01f90 14 24 this this DET 0k225b01f90 14 25 work work NOUN 0k225b01f90 14 26 implicate implicate VERB 0k225b01f90 14 27 spindly spindly ADV 0k225b01f90 14 28 in in ADP 0k225b01f90 14 29 a a DET 0k225b01f90 14 30 novel novel ADJ 0k225b01f90 14 31 dynactin dynactin NOUN 0k225b01f90 14 32 - - PUNCT 0k225b01f90 14 33 independent independent ADJ 0k225b01f90 14 34 hand hand NOUN 0k225b01f90 14 35 - - PUNCT 0k225b01f90 14 36 off off ADP 0k225b01f90 14 37 mechanism mechanism NOUN 0k225b01f90 14 38 at at ADP 0k225b01f90 14 39 kinetochores kinetochore NOUN 0k225b01f90 14 40 in in ADP 0k225b01f90 14 41 which which PRON 0k225b01f90 14 42 spindly spindly ADV 0k225b01f90 14 43 is be AUX 0k225b01f90 14 44 required require VERB 0k225b01f90 14 45 for for ADP 0k225b01f90 14 46 dynein dynein NOUN 0k225b01f90 14 47 poleward poleward ADJ 0k225b01f90 14 48 motility motility NOUN 0k225b01f90 14 49 . . PUNCT 0k225b01f90 15 1 overall overall ADV 0k225b01f90 15 2 , , PUNCT 0k225b01f90 15 3 these these DET 0k225b01f90 15 4 findings finding NOUN 0k225b01f90 15 5 provide provide VERB 0k225b01f90 15 6 a a DET 0k225b01f90 15 7 more more ADV 0k225b01f90 15 8 thorough thorough ADJ 0k225b01f90 15 9 understanding understanding NOUN 0k225b01f90 15 10 of of ADP 0k225b01f90 15 11 the the DET 0k225b01f90 15 12 role role NOUN 0k225b01f90 15 13 of of ADP 0k225b01f90 15 14 phosphorylation phosphorylation NOUN 0k225b01f90 15 15 in in ADP 0k225b01f90 15 16 dynein dynein NOUN 0k225b01f90 15 17 differential differential ADJ 0k225b01f90 15 18 targeting targeting NOUN 0k225b01f90 15 19 and and CCONJ 0k225b01f90 15 20 function function NOUN 0k225b01f90 15 21 during during ADP 0k225b01f90 15 22 mitosis mitosis NOUN 0k225b01f90 15 23 . . PUNCT