id author title date pages extension mime words sentence flesch summary cache txt h989r210w6s Jessica K Lukowski Mass Spectrometry-Based Spatial Analysis of Endogenous and Exogenous Molecules in Three-Dimensional Cell Culture 2019 .txt text/plain 339 14 35 These multicellular aggregates referred to as tumor spheroids, display pathophysiological gradients that are more complex than conventional two-dimensional cell cultures in addition to being higher throughput than animal models. However, the techniques utilized within this document include Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI MSI) and Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) followed by liquid chromatography tandem mass spectrometry (LC-MS/MS).Tumors in vivo have a complex microenvironment and researchers have spent decades developing different models to test the efficacy of novel treatments. cache/h989r210w6s.txt txt/h989r210w6s.txt