id author title date pages extension mime words sentence flesch summary cache txt f7623b62879 Jin Jia Spatiotemporal Mapping of Quinolone and Phenazine Secretion in Pseudomonas aeruginosa Biofilms by Confocal Raman Imaging 2021 .txt text/plain 619 18 21 Confocal Raman microscopy was also applied to investigate the response of P. aeruginosa biofilm grown on lithographically defined patterned or unpatterned mucin to antibiotic treatment and to characterize the spatial and temporal distribution of secreted metabolites from static biofilms on the substrate, from supernatant of the bacterial broth, and from pellicle biofilms at the air-liquid interface. The work described in this thesis uses multimodal chemical imaging – Raman microspectroscopy, mass spectrometric imaging and electrochemical measurements - together with micro- and nanofabricated structures of high spatial definition to investigate the mechanisms of P. aeruginosa biofilm growth by characterizing the spatial and temporal distributions of signaling molecules and other secreted metabolites. cache/f7623b62879.txt txt/f7623b62879.txt