id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
5425k933b6v	Liu Yang	Membrane Modification for Ion Separations and Drug-target Identification	2020		.txt	text/plain	302	11	32	Selective elution of captured protein, tryptic digestion, tandem mass spectrometry analysis, and label-free quantification (LFQ) identify CAII as the dominant AEBSA target in diluted serum or cell lysate Global proteomics shows that the spiked CAII is the only protein with a log2 ratio (sample/control intensity) consistently >2, and the detection limit for CAII identification is 0.004 wt% of the total protein in 1:4 diluted human serum or 0.024 wt% of the total protein from breast cancer cell lysates. A model system of carbonic anhydrase II (CAII) binding to immobilized 4-(2-aminoethyl)benzenesulfonamide (AEBSA) demonstrates the efficiency and selectivity of affinity capture in modified membranes.	cache/5425k933b6v.txt	txt/5425k933b6v.txt