id sid tid token lemma pos 10_1101-2021_02_08_430275 1 1 Next next JJ 10_1101-2021_02_08_430275 1 2 - - HYPH 10_1101-2021_02_08_430275 1 3 generation generation NN 10_1101-2021_02_08_430275 1 4 sequencing sequencing NN 10_1101-2021_02_08_430275 1 5 - - HYPH 10_1101-2021_02_08_430275 1 6 based base VBN 10_1101-2021_02_08_430275 1 7 bulked bulk VBN 10_1101-2021_02_08_430275 1 8 segregant segregant JJ 10_1101-2021_02_08_430275 1 9 analysis analysis NN 10_1101-2021_02_08_430275 1 10 without without IN 10_1101-2021_02_08_430275 1 11 sequencing sequence VBG 10_1101-2021_02_08_430275 1 12 the the DT 10_1101-2021_02_08_430275 1 13 parental parental JJ 10_1101-2021_02_08_430275 1 14 genomes genome NNS 10_1101-2021_02_08_430275 1 15 DR DR NNP 10_1101-2021_02_08_430275 1 16 AF AF NNP 10_1101-2021_02_08_430275 1 17 T T NNP 10_1101-2021_02_08_430275 1 18 Next Next NNP 10_1101-2021_02_08_430275 1 19 - - HYPH 10_1101-2021_02_08_430275 1 20 generation generation NN 10_1101-2021_02_08_430275 1 21 sequencing sequencing NN 10_1101-2021_02_08_430275 1 22 - - HYPH 10_1101-2021_02_08_430275 1 23 based base VBN 10_1101-2021_02_08_430275 1 24 bulked bulk VBN 10_1101-2021_02_08_430275 1 25 segregant segregant JJ 10_1101-2021_02_08_430275 1 26 analysis analysis NN 10_1101-2021_02_08_430275 1 27 without without IN 10_1101-2021_02_08_430275 1 28 sequencing sequence VBG 10_1101-2021_02_08_430275 1 29 the the DT 10_1101-2021_02_08_430275 1 30 parental parental JJ 10_1101-2021_02_08_430275 1 31 genomes genome NNS 10_1101-2021_02_08_430275 1 32 Jianbo Jianbo NNP 10_1101-2021_02_08_430275 1 33 Zhanga Zhanga NNP 10_1101-2021_02_08_430275 1 34 , , , 10_1101-2021_02_08_430275 1 35 � � NNP 10_1101-2021_02_08_430275 1 36 and and CC 10_1101-2021_02_08_430275 1 37 Dilip Dilip NNP 10_1101-2021_02_08_430275 1 38 R. R. NNP 10_1101-2021_02_08_430275 1 39 Pantheea Pantheea NNP 10_1101-2021_02_08_430275 1 40 , , , 10_1101-2021_02_08_430275 1 41 � � NNP 10_1101-2021_02_08_430275 1 42 a a DT 10_1101-2021_02_08_430275 1 43 Department Department NNP 10_1101-2021_02_08_430275 1 44 of of IN 10_1101-2021_02_08_430275 1 45 Horticultural Horticultural NNP 10_1101-2021_02_08_430275 1 46 Science Science NNP 10_1101-2021_02_08_430275 1 47 , , , 10_1101-2021_02_08_430275 1 48 North North NNP 10_1101-2021_02_08_430275 1 49 Carolina Carolina NNP 10_1101-2021_02_08_430275 1 50 State State NNP 10_1101-2021_02_08_430275 1 51 University University NNP 10_1101-2021_02_08_430275 1 52 , , , 10_1101-2021_02_08_430275 1 53 Mountain Mountain NNP 10_1101-2021_02_08_430275 1 54 Horticultural Horticultural NNP 10_1101-2021_02_08_430275 1 55 Crops Crops NNP 10_1101-2021_02_08_430275 1 56 Research Research NNP 10_1101-2021_02_08_430275 1 57 and and CC 10_1101-2021_02_08_430275 1 58 Extension Extension NNP 10_1101-2021_02_08_430275 1 59 Center Center NNP 10_1101-2021_02_08_430275 1 60 , , , 10_1101-2021_02_08_430275 1 61 455 455 CD 10_1101-2021_02_08_430275 1 62 Research Research NNP 10_1101-2021_02_08_430275 1 63 Drive Drive NNP 10_1101-2021_02_08_430275 1 64 , , , 10_1101-2021_02_08_430275 1 65 Mills Mills NNP 10_1101-2021_02_08_430275 1 66 River River NNP 10_1101-2021_02_08_430275 1 67 , , , 10_1101-2021_02_08_430275 1 68 NC NC NNP 10_1101-2021_02_08_430275 1 69 28759 28759 CD 10_1101-2021_02_08_430275 1 70 , , , 10_1101-2021_02_08_430275 1 71 USA USA NNP 10_1101-2021_02_08_430275 1 72 This this DT 10_1101-2021_02_08_430275 1 73 manuscript manuscript NN 10_1101-2021_02_08_430275 1 74 was be VBD 10_1101-2021_02_08_430275 1 75 compiled compile VBN 10_1101-2021_02_08_430275 1 76 on on IN 10_1101-2021_02_08_430275 1 77 November November NNP 10_1101-2021_02_08_430275 1 78 24 24 CD 10_1101-2021_02_08_430275 1 79 , , , 10_1101-2021_02_08_430275 1 80 2020 2020 CD 10_1101-2021_02_08_430275 1 81 The the DT 10_1101-2021_02_08_430275 1 82 genomic genomic JJ 10_1101-2021_02_08_430275 1 83 region(s region(s NN 10_1101-2021_02_08_430275 1 84 ) ) -RRB- 10_1101-2021_02_08_430275 1 85 that that WDT 10_1101-2021_02_08_430275 1 86 controls control VBZ 10_1101-2021_02_08_430275 1 87 a a DT 10_1101-2021_02_08_430275 1 88 trait trait NN 10_1101-2021_02_08_430275 1 89 of of IN 10_1101-2021_02_08_430275 1 90 interest interest NN 10_1101-2021_02_08_430275 1 91 can can MD 10_1101-2021_02_08_430275 1 92 be be VB 10_1101-2021_02_08_430275 1 93 rapidly rapidly RB 10_1101-2021_02_08_430275 1 94 identified identify VBN 10_1101-2021_02_08_430275 1 95 using use VBG 10_1101-2021_02_08_430275 1 96 BSA BSA NNP 10_1101-2021_02_08_430275 1 97 - - HYPH 10_1101-2021_02_08_430275 1 98 Seq Seq NNP 10_1101-2021_02_08_430275 1 99 , , , 10_1101-2021_02_08_430275 1 100 a a DT 10_1101-2021_02_08_430275 1 101 technology technology NN 10_1101-2021_02_08_430275 1 102 in in IN 10_1101-2021_02_08_430275 1 103 which which WDT 10_1101-2021_02_08_430275 1 104 next next JJ 10_1101-2021_02_08_430275 1 105 - - HYPH 10_1101-2021_02_08_430275 1 106 generation generation NN 10_1101-2021_02_08_430275 1 107 se- se- NN 10_1101-2021_02_08_430275 1 108 quencing quencing NN 10_1101-2021_02_08_430275 1 109 ( ( -LRB- 10_1101-2021_02_08_430275 1 110 NGS NGS NNP 10_1101-2021_02_08_430275 1 111 ) ) -RRB- 10_1101-2021_02_08_430275 1 112 is be VBZ 10_1101-2021_02_08_430275 1 113 applied apply VBN 10_1101-2021_02_08_430275 1 114 to to IN 10_1101-2021_02_08_430275 1 115 bulked bulk VBN 10_1101-2021_02_08_430275 1 116 segregant segregant JJ 10_1101-2021_02_08_430275 1 117 analysis analysis NN 10_1101-2021_02_08_430275 1 118 ( ( -LRB- 10_1101-2021_02_08_430275 1 119 BSA BSA NNP 10_1101-2021_02_08_430275 1 120 ) ) -RRB- 10_1101-2021_02_08_430275 1 121 . . . 10_1101-2021_02_08_430275 2 1 We -PRON- PRP 10_1101-2021_02_08_430275 2 2 recently recently RB 10_1101-2021_02_08_430275 2 3 developed develop VBD 10_1101-2021_02_08_430275 2 4 the the DT 10_1101-2021_02_08_430275 2 5 significant significant JJ 10_1101-2021_02_08_430275 2 6 structural structural JJ 10_1101-2021_02_08_430275 2 7 variant variant JJ 10_1101-2021_02_08_430275 2 8 method method NN 10_1101-2021_02_08_430275 2 9 for for IN 10_1101-2021_02_08_430275 2 10 BSA- BSA- NNP 10_1101-2021_02_08_430275 2 11 Seq Seq NNP 10_1101-2021_02_08_430275 2 12 data data NN 10_1101-2021_02_08_430275 2 13 analysis analysis NN 10_1101-2021_02_08_430275 2 14 that that WDT 10_1101-2021_02_08_430275 2 15 exhibits exhibit VBZ 10_1101-2021_02_08_430275 2 16 higher high JJR 10_1101-2021_02_08_430275 2 17 detection detection NN 10_1101-2021_02_08_430275 2 18 power power NN 10_1101-2021_02_08_430275 2 19 than than IN 10_1101-2021_02_08_430275 2 20 standard standard JJ 10_1101-2021_02_08_430275 2 21 BSA BSA NNP 10_1101-2021_02_08_430275 2 22 - - HYPH 10_1101-2021_02_08_430275 2 23 Seq Seq NNP 10_1101-2021_02_08_430275 2 24 analysis analysis NN 10_1101-2021_02_08_430275 2 25 methods method NNS 10_1101-2021_02_08_430275 2 26 . . . 10_1101-2021_02_08_430275 3 1 Our -PRON- PRP$ 10_1101-2021_02_08_430275 3 2 original original JJ 10_1101-2021_02_08_430275 3 3 algorithm algorithm NN 10_1101-2021_02_08_430275 3 4 was be VBD 10_1101-2021_02_08_430275 3 5 developed develop VBN 10_1101-2021_02_08_430275 3 6 to to TO 10_1101-2021_02_08_430275 3 7 analyze analyze VB 10_1101-2021_02_08_430275 3 8 BSA BSA NNP 10_1101-2021_02_08_430275 3 9 - - HYPH 10_1101-2021_02_08_430275 3 10 Seq seq NN 10_1101-2021_02_08_430275 3 11 data datum NNS 10_1101-2021_02_08_430275 3 12 in in IN 10_1101-2021_02_08_430275 3 13 which which WDT 10_1101-2021_02_08_430275 3 14 genome genome JJ 10_1101-2021_02_08_430275 3 15 sequences sequence NNS 10_1101-2021_02_08_430275 3 16 of of IN 10_1101-2021_02_08_430275 3 17 one one CD 10_1101-2021_02_08_430275 3 18 par- par- NN 10_1101-2021_02_08_430275 3 19 ent ent NN 10_1101-2021_02_08_430275 3 20 served serve VBD 10_1101-2021_02_08_430275 3 21 as as IN 10_1101-2021_02_08_430275 3 22 the the DT 10_1101-2021_02_08_430275 3 23 reference reference NN 10_1101-2021_02_08_430275 3 24 sequences sequence VBZ 10_1101-2021_02_08_430275 3 25 in in IN 10_1101-2021_02_08_430275 3 26 genotype genotype NN 10_1101-2021_02_08_430275 3 27 calling calling NN 10_1101-2021_02_08_430275 3 28 , , , 10_1101-2021_02_08_430275 3 29 and and CC 10_1101-2021_02_08_430275 3 30 thus thus RB 10_1101-2021_02_08_430275 3 31 required require VBD 10_1101-2021_02_08_430275 3 32 the the DT 10_1101-2021_02_08_430275 3 33 availability availability NN 10_1101-2021_02_08_430275 3 34 of of IN 10_1101-2021_02_08_430275 3 35 high high JJ 10_1101-2021_02_08_430275 3 36 - - HYPH 10_1101-2021_02_08_430275 3 37 quality quality NN 10_1101-2021_02_08_430275 3 38 assembled assemble VBN 10_1101-2021_02_08_430275 3 39 parental parental JJ 10_1101-2021_02_08_430275 3 40 genome genome NN 10_1101-2021_02_08_430275 3 41 sequences sequence NNS 10_1101-2021_02_08_430275 3 42 . . . 10_1101-2021_02_08_430275 4 1 Here here RB 10_1101-2021_02_08_430275 4 2 we -PRON- PRP 10_1101-2021_02_08_430275 4 3 modified modify VBD 10_1101-2021_02_08_430275 4 4 the the DT 10_1101-2021_02_08_430275 4 5 original original JJ 10_1101-2021_02_08_430275 4 6 script script NN 10_1101-2021_02_08_430275 4 7 to to TO 10_1101-2021_02_08_430275 4 8 allow allow VB 10_1101-2021_02_08_430275 4 9 for for IN 10_1101-2021_02_08_430275 4 10 the the DT 10_1101-2021_02_08_430275 4 11 ef- ef- NN 10_1101-2021_02_08_430275 4 12 fective fective JJ 10_1101-2021_02_08_430275 4 13 detection detection NN 10_1101-2021_02_08_430275 4 14 of of IN 10_1101-2021_02_08_430275 4 15 the the DT 10_1101-2021_02_08_430275 4 16 genomic genomic JJ 10_1101-2021_02_08_430275 4 17 region region NN 10_1101-2021_02_08_430275 4 18 - - HYPH 10_1101-2021_02_08_430275 4 19 trait trait NN 10_1101-2021_02_08_430275 4 20 associations association NNS 10_1101-2021_02_08_430275 4 21 using use VBG 10_1101-2021_02_08_430275 4 22 only only JJ 10_1101-2021_02_08_430275 4 23 bulk bulk JJ 10_1101-2021_02_08_430275 4 24 genome genome JJ 10_1101-2021_02_08_430275 4 25 sequences sequence NNS 10_1101-2021_02_08_430275 4 26 . . . 10_1101-2021_02_08_430275 5 1 We -PRON- PRP 10_1101-2021_02_08_430275 5 2 analyzed analyze VBD 10_1101-2021_02_08_430275 5 3 a a DT 10_1101-2021_02_08_430275 5 4 public public JJ 10_1101-2021_02_08_430275 5 5 BSA BSA NNP 10_1101-2021_02_08_430275 5 6 - - HYPH 10_1101-2021_02_08_430275 5 7 Seq Seq NNP 10_1101-2021_02_08_430275 5 8 dataset dataset NN 10_1101-2021_02_08_430275 5 9 us- us- XX 10_1101-2021_02_08_430275 5 10 ing e VBG 10_1101-2021_02_08_430275 5 11 our -PRON- PRP$ 10_1101-2021_02_08_430275 5 12 modified modify VBN 10_1101-2021_02_08_430275 5 13 method method NN 10_1101-2021_02_08_430275 5 14 and and CC 10_1101-2021_02_08_430275 5 15 the the DT 10_1101-2021_02_08_430275 5 16 standard standard JJ 10_1101-2021_02_08_430275 5 17 allele allele NNP 10_1101-2021_02_08_430275 5 18 frequency frequency NN 10_1101-2021_02_08_430275 5 19 and and CC 10_1101-2021_02_08_430275 5 20 G- g- JJ 10_1101-2021_02_08_430275 5 21 statistic statistic JJ 10_1101-2021_02_08_430275 5 22 methods method NNS 10_1101-2021_02_08_430275 5 23 with with IN 10_1101-2021_02_08_430275 5 24 and and CC 10_1101-2021_02_08_430275 5 25 without without IN 10_1101-2021_02_08_430275 5 26 the the DT 10_1101-2021_02_08_430275 5 27 aid aid NN 10_1101-2021_02_08_430275 5 28 of of IN 10_1101-2021_02_08_430275 5 29 the the DT 10_1101-2021_02_08_430275 5 30 parental parental JJ 10_1101-2021_02_08_430275 5 31 genome genome JJ 10_1101-2021_02_08_430275 5 32 sequences sequence NNS 10_1101-2021_02_08_430275 5 33 . . . 10_1101-2021_02_08_430275 6 1 Our -PRON- PRP$ 10_1101-2021_02_08_430275 6 2 results result NNS 10_1101-2021_02_08_430275 6 3 demonstrate demonstrate VBP 10_1101-2021_02_08_430275 6 4 that that IN 10_1101-2021_02_08_430275 6 5 the the DT 10_1101-2021_02_08_430275 6 6 genomic genomic NN 10_1101-2021_02_08_430275 6 7 region(s region(s NNP 10_1101-2021_02_08_430275 6 8 ) ) -RRB- 10_1101-2021_02_08_430275 6 9 as- as- NNP 10_1101-2021_02_08_430275 6 10 sociated sociate VBD 10_1101-2021_02_08_430275 6 11 with with IN 10_1101-2021_02_08_430275 6 12 the the DT 10_1101-2021_02_08_430275 6 13 trait trait NN 10_1101-2021_02_08_430275 6 14 of of IN 10_1101-2021_02_08_430275 6 15 interest interest NN 10_1101-2021_02_08_430275 6 16 could could MD 10_1101-2021_02_08_430275 6 17 be be VB 10_1101-2021_02_08_430275 6 18 reliably reliably RB 10_1101-2021_02_08_430275 6 19 identified identify VBN 10_1101-2021_02_08_430275 6 20 only only RB 10_1101-2021_02_08_430275 6 21 via via IN 10_1101-2021_02_08_430275 6 22 the the DT 10_1101-2021_02_08_430275 6 23 significant significant JJ 10_1101-2021_02_08_430275 6 24 structural structural JJ 10_1101-2021_02_08_430275 6 25 variant variant JJ 10_1101-2021_02_08_430275 6 26 method method NN 10_1101-2021_02_08_430275 6 27 without without IN 10_1101-2021_02_08_430275 6 28 using use VBG 10_1101-2021_02_08_430275 6 29 the the DT 10_1101-2021_02_08_430275 6 30 parental parental JJ 10_1101-2021_02_08_430275 6 31 genome genome JJ 10_1101-2021_02_08_430275 6 32 sequences sequence NNS 10_1101-2021_02_08_430275 6 33 . . . 10_1101-2021_02_08_430275 7 1 1 1 CD 10_1101-2021_02_08_430275 7 2 2 2 CD 10_1101-2021_02_08_430275 7 3 3 3 CD 10_1101-2021_02_08_430275 7 4 4 4 CD 10_1101-2021_02_08_430275 7 5 5 5 CD 10_1101-2021_02_08_430275 7 6 6 6 CD 10_1101-2021_02_08_430275 7 7 7 7 CD 10_1101-2021_02_08_430275 7 8 8 8 CD 10_1101-2021_02_08_430275 7 9 9 9 CD 10_1101-2021_02_08_430275 7 10 10 10 CD 10_1101-2021_02_08_430275 7 11 11 11 CD 10_1101-2021_02_08_430275 7 12 12 12 CD 10_1101-2021_02_08_430275 7 13 13 13 CD 10_1101-2021_02_08_430275 7 14 14 14 CD 10_1101-2021_02_08_430275 7 15 15 15 CD 10_1101-2021_02_08_430275 7 16 16 16 CD 10_1101-2021_02_08_430275 7 17 17 17 CD 10_1101-2021_02_08_430275 7 18 18 18 CD 10_1101-2021_02_08_430275 7 19 BSA BSA NNP 10_1101-2021_02_08_430275 7 20 - - HYPH 10_1101-2021_02_08_430275 7 21 Seq Seq NNP 10_1101-2021_02_08_430275 7 22 | | NNP 10_1101-2021_02_08_430275 7 23 PyBSASeq pybsaseq CD 10_1101-2021_02_08_430275 7 24 | | NNP 10_1101-2021_02_08_430275 7 25 QTL QTL NNP 10_1101-2021_02_08_430275 7 26 | | CD 10_1101-2021_02_08_430275 7 27 genomic genomic JJ 10_1101-2021_02_08_430275 7 28 region region NN 10_1101-2021_02_08_430275 7 29 - - HYPH 10_1101-2021_02_08_430275 7 30 trait trait NN 10_1101-2021_02_08_430275 7 31 association association NN 10_1101-2021_02_08_430275 7 32 Bulked bulked JJ 10_1101-2021_02_08_430275 7 33 segregant segregant JJ 10_1101-2021_02_08_430275 7 34 analysis analysis NN 10_1101-2021_02_08_430275 7 35 ( ( -LRB- 10_1101-2021_02_08_430275 7 36 BSA BSA NNP 10_1101-2021_02_08_430275 7 37 ) ) -RRB- 10_1101-2021_02_08_430275 7 38 was be VBD 10_1101-2021_02_08_430275 7 39 developed develop VBN 10_1101-2021_02_08_430275 7 40 for for IN 10_1101-2021_02_08_430275 7 41 the1 the1 DT 10_1101-2021_02_08_430275 7 42 quick quick JJ 10_1101-2021_02_08_430275 7 43 identification identification NN 10_1101-2021_02_08_430275 7 44 of of IN 10_1101-2021_02_08_430275 7 45 genetic genetic JJ 10_1101-2021_02_08_430275 7 46 markers marker NNS 10_1101-2021_02_08_430275 7 47 associated associate VBN 10_1101-2021_02_08_430275 7 48 with with IN 10_1101-2021_02_08_430275 7 49 a2 a2 NNP 10_1101-2021_02_08_430275 7 50 trait trait NN 10_1101-2021_02_08_430275 7 51 of of IN 10_1101-2021_02_08_430275 7 52 interest interest NN 10_1101-2021_02_08_430275 7 53 ( ( -LRB- 10_1101-2021_02_08_430275 7 54 1 1 CD 10_1101-2021_02_08_430275 7 55 , , , 10_1101-2021_02_08_430275 7 56 2 2 CD 10_1101-2021_02_08_430275 7 57 ) ) -RRB- 10_1101-2021_02_08_430275 7 58 . . . 10_1101-2021_02_08_430275 8 1 For for IN 10_1101-2021_02_08_430275 8 2 a a DT 10_1101-2021_02_08_430275 8 3 particular particular JJ 10_1101-2021_02_08_430275 8 4 trait trait NN 10_1101-2021_02_08_430275 8 5 , , , 10_1101-2021_02_08_430275 8 6 two two CD 10_1101-2021_02_08_430275 8 7 groups group NNS 10_1101-2021_02_08_430275 8 8 of3 of3 VBP 10_1101-2021_02_08_430275 8 9 individuals individual NNS 10_1101-2021_02_08_430275 8 10 with with IN 10_1101-2021_02_08_430275 8 11 contrasting contrast VBG 10_1101-2021_02_08_430275 8 12 phenotypes phenotype NNS 10_1101-2021_02_08_430275 8 13 are be VBP 10_1101-2021_02_08_430275 8 14 selected select VBN 10_1101-2021_02_08_430275 8 15 from from IN 10_1101-2021_02_08_430275 8 16 a4 a4 NN 10_1101-2021_02_08_430275 8 17 segregating segregate VBG 10_1101-2021_02_08_430275 8 18 population population NN 10_1101-2021_02_08_430275 8 19 . . . 10_1101-2021_02_08_430275 9 1 Equal equal JJ 10_1101-2021_02_08_430275 9 2 amounts amount NNS 10_1101-2021_02_08_430275 9 3 of of IN 10_1101-2021_02_08_430275 9 4 DNA dna NN 10_1101-2021_02_08_430275 9 5 are be VBP 10_1101-2021_02_08_430275 9 6 pooled5 pooled5 NN 10_1101-2021_02_08_430275 9 7 from from IN 10_1101-2021_02_08_430275 9 8 each each DT 10_1101-2021_02_08_430275 9 9 individual individual NN 10_1101-2021_02_08_430275 9 10 within within IN 10_1101-2021_02_08_430275 9 11 a a DT 10_1101-2021_02_08_430275 9 12 group group NN 10_1101-2021_02_08_430275 9 13 . . . 10_1101-2021_02_08_430275 10 1 The the DT 10_1101-2021_02_08_430275 10 2 pooled pool VBN 10_1101-2021_02_08_430275 10 3 DNA dna NN 10_1101-2021_02_08_430275 10 4 samples6 samples6 NN 10_1101-2021_02_08_430275 10 5 are be VBP 10_1101-2021_02_08_430275 10 6 then then RB 10_1101-2021_02_08_430275 10 7 subjected subject VBN 10_1101-2021_02_08_430275 10 8 to to IN 10_1101-2021_02_08_430275 10 9 analysis analysis NN 10_1101-2021_02_08_430275 10 10 , , , 10_1101-2021_02_08_430275 10 11 such such JJ 10_1101-2021_02_08_430275 10 12 as as IN 10_1101-2021_02_08_430275 10 13 restriction restriction NN 10_1101-2021_02_08_430275 10 14 fragment7 fragment7 VBZ 10_1101-2021_02_08_430275 10 15 length length NN 10_1101-2021_02_08_430275 10 16 polymorphism polymorphism NN 10_1101-2021_02_08_430275 10 17 ( ( -LRB- 10_1101-2021_02_08_430275 10 18 RFLP RFLP NNP 10_1101-2021_02_08_430275 10 19 ) ) -RRB- 10_1101-2021_02_08_430275 10 20 or or CC 10_1101-2021_02_08_430275 10 21 random random JJ 10_1101-2021_02_08_430275 10 22 amplification amplification NN 10_1101-2021_02_08_430275 10 23 of of IN 10_1101-2021_02_08_430275 10 24 poly-8 poly-8 NNP 10_1101-2021_02_08_430275 10 25 morphic morphic JJ 10_1101-2021_02_08_430275 10 26 DNA DNA NNP 10_1101-2021_02_08_430275 10 27 ( ( -LRB- 10_1101-2021_02_08_430275 10 28 RAPD RAPD NNP 10_1101-2021_02_08_430275 10 29 ) ) -RRB- 10_1101-2021_02_08_430275 10 30 . . . 10_1101-2021_02_08_430275 11 1 Fragments fragment VBZ 10_1101-2021_02_08_430275 11 2 unique unique JJ 10_1101-2021_02_08_430275 11 3 to to IN 10_1101-2021_02_08_430275 11 4 either either DT 10_1101-2021_02_08_430275 11 5 group group NN 10_1101-2021_02_08_430275 11 6 are9 are9 JJ 10_1101-2021_02_08_430275 11 7 potential potential JJ 10_1101-2021_02_08_430275 11 8 genetic genetic JJ 10_1101-2021_02_08_430275 11 9 markers marker NNS 10_1101-2021_02_08_430275 11 10 that that WDT 10_1101-2021_02_08_430275 11 11 may may MD 10_1101-2021_02_08_430275 11 12 link link VB 10_1101-2021_02_08_430275 11 13 to to IN 10_1101-2021_02_08_430275 11 14 the the DT 10_1101-2021_02_08_430275 11 15 gene(s gene(s NNP 10_1101-2021_02_08_430275 11 16 ) ) -RRB- 10_1101-2021_02_08_430275 11 17 that10 that10 NNP 10_1101-2021_02_08_430275 11 18 control control NNP 10_1101-2021_02_08_430275 11 19 phenotypic phenotypic NNP 10_1101-2021_02_08_430275 11 20 expression expression NN 10_1101-2021_02_08_430275 11 21 for for IN 10_1101-2021_02_08_430275 11 22 the the DT 10_1101-2021_02_08_430275 11 23 trait trait NN 10_1101-2021_02_08_430275 11 24 of of IN 10_1101-2021_02_08_430275 11 25 interest interest NN 10_1101-2021_02_08_430275 11 26 . . . 10_1101-2021_02_08_430275 12 1 Can-11 can-11 JJ 10_1101-2021_02_08_430275 12 2 didate didate NN 10_1101-2021_02_08_430275 12 3 markers marker NNS 10_1101-2021_02_08_430275 12 4 are be VBP 10_1101-2021_02_08_430275 12 5 further further RB 10_1101-2021_02_08_430275 12 6 tested test VBN 10_1101-2021_02_08_430275 12 7 against against IN 10_1101-2021_02_08_430275 12 8 the the DT 10_1101-2021_02_08_430275 12 9 population population NN 10_1101-2021_02_08_430275 12 10 to12 to12 NNP 10_1101-2021_02_08_430275 12 11 verify verify VBP 10_1101-2021_02_08_430275 12 12 the the DT 10_1101-2021_02_08_430275 12 13 marker marker NN 10_1101-2021_02_08_430275 12 14 - - HYPH 10_1101-2021_02_08_430275 12 15 trait trait NN 10_1101-2021_02_08_430275 12 16 associations association NNS 10_1101-2021_02_08_430275 12 17 . . . 10_1101-2021_02_08_430275 13 1 With with IN 10_1101-2021_02_08_430275 13 2 the the DT 10_1101-2021_02_08_430275 13 3 recent recent JJ 10_1101-2021_02_08_430275 13 4 dramatic13 dramatic13 JJ 10_1101-2021_02_08_430275 13 5 reductions reduction NNS 10_1101-2021_02_08_430275 13 6 in in IN 10_1101-2021_02_08_430275 13 7 cost cost NN 10_1101-2021_02_08_430275 13 8 , , , 10_1101-2021_02_08_430275 13 9 next next JJ 10_1101-2021_02_08_430275 13 10 - - HYPH 10_1101-2021_02_08_430275 13 11 generation generation NN 10_1101-2021_02_08_430275 13 12 sequencing sequencing NN 10_1101-2021_02_08_430275 13 13 ( ( -LRB- 10_1101-2021_02_08_430275 13 14 NGS NGS NNP 10_1101-2021_02_08_430275 13 15 ) ) -RRB- 10_1101-2021_02_08_430275 13 16 has have VBZ 10_1101-2021_02_08_430275 13 17 been14 been14 VBN 10_1101-2021_02_08_430275 13 18 applied apply VBN 10_1101-2021_02_08_430275 13 19 to to IN 10_1101-2021_02_08_430275 13 20 more more JJR 10_1101-2021_02_08_430275 13 21 and and CC 10_1101-2021_02_08_430275 13 22 more more JJR 10_1101-2021_02_08_430275 13 23 BSA BSA NNP 10_1101-2021_02_08_430275 13 24 studies study NNS 10_1101-2021_02_08_430275 13 25 ( ( -LRB- 10_1101-2021_02_08_430275 13 26 3–7 3–7 CD 10_1101-2021_02_08_430275 13 27 ) ) -RRB- 10_1101-2021_02_08_430275 13 28 . . . 10_1101-2021_02_08_430275 14 1 This this DT 10_1101-2021_02_08_430275 14 2 new new JJ 10_1101-2021_02_08_430275 14 3 tech-15 tech-15 NN 10_1101-2021_02_08_430275 14 4 nology nology NN 10_1101-2021_02_08_430275 14 5 is be VBZ 10_1101-2021_02_08_430275 14 6 referred refer VBN 10_1101-2021_02_08_430275 14 7 to to IN 10_1101-2021_02_08_430275 14 8 as as IN 10_1101-2021_02_08_430275 14 9 BSA BSA NNP 10_1101-2021_02_08_430275 14 10 - - HYPH 10_1101-2021_02_08_430275 14 11 Seq seq NN 10_1101-2021_02_08_430275 14 12 . . . 10_1101-2021_02_08_430275 15 1 In in IN 10_1101-2021_02_08_430275 15 2 BSA BSA NNP 10_1101-2021_02_08_430275 15 3 - - HYPH 10_1101-2021_02_08_430275 15 4 Seq Seq NNP 10_1101-2021_02_08_430275 15 5 , , , 10_1101-2021_02_08_430275 15 6 pooled pool VBN 10_1101-2021_02_08_430275 15 7 DNA16 DNA16 NNP 10_1101-2021_02_08_430275 15 8 samples sample NNS 10_1101-2021_02_08_430275 15 9 are be VBP 10_1101-2021_02_08_430275 15 10 not not RB 10_1101-2021_02_08_430275 15 11 subjected subject VBN 10_1101-2021_02_08_430275 15 12 to to IN 10_1101-2021_02_08_430275 15 13 RFLP RFLP NNP 10_1101-2021_02_08_430275 15 14 / / SYM 10_1101-2021_02_08_430275 15 15 RAPD RAPD NNP 10_1101-2021_02_08_430275 15 16 analysis analysis NN 10_1101-2021_02_08_430275 15 17 , , , 10_1101-2021_02_08_430275 15 18 but but CC 10_1101-2021_02_08_430275 15 19 are17 are17 ADD 10_1101-2021_02_08_430275 15 20 directly directly RB 10_1101-2021_02_08_430275 15 21 sequenced sequence VBN 10_1101-2021_02_08_430275 15 22 instead instead RB 10_1101-2021_02_08_430275 15 23 . . . 10_1101-2021_02_08_430275 16 1 Genome genome JJ 10_1101-2021_02_08_430275 16 2 - - HYPH 10_1101-2021_02_08_430275 16 3 wide wide JJ 10_1101-2021_02_08_430275 16 4 structural structural JJ 10_1101-2021_02_08_430275 16 5 variants18 variants18 NNS 10_1101-2021_02_08_430275 16 6 between between IN 10_1101-2021_02_08_430275 16 7 bulks bulk NNS 10_1101-2021_02_08_430275 16 8 , , , 10_1101-2021_02_08_430275 16 9 such such JJ 10_1101-2021_02_08_430275 16 10 as as IN 10_1101-2021_02_08_430275 16 11 single single JJ 10_1101-2021_02_08_430275 16 12 nucleotide nucleotide JJ 10_1101-2021_02_08_430275 16 13 polymorphisms polymorphism NNS 10_1101-2021_02_08_430275 16 14 ( ( -LRB- 10_1101-2021_02_08_430275 16 15 SNP)19 snp)19 NN 10_1101-2021_02_08_430275 16 16 and and CC 10_1101-2021_02_08_430275 16 17 small small JJ 10_1101-2021_02_08_430275 16 18 insertions insertion NNS 10_1101-2021_02_08_430275 16 19 / / SYM 10_1101-2021_02_08_430275 16 20 deletions deletion NNS 10_1101-2021_02_08_430275 16 21 ( ( -LRB- 10_1101-2021_02_08_430275 16 22 InDel InDel NNP 10_1101-2021_02_08_430275 16 23 ) ) -RRB- 10_1101-2021_02_08_430275 16 24 , , , 10_1101-2021_02_08_430275 16 25 are be VBP 10_1101-2021_02_08_430275 16 26 identified identify VBN 10_1101-2021_02_08_430275 16 27 based20 based20 FW 10_1101-2021_02_08_430275 16 28 on on IN 10_1101-2021_02_08_430275 16 29 the the DT 10_1101-2021_02_08_430275 16 30 sequencing sequencing NN 10_1101-2021_02_08_430275 16 31 data datum NNS 10_1101-2021_02_08_430275 16 32 . . . 10_1101-2021_02_08_430275 17 1 Genomic genomic JJ 10_1101-2021_02_08_430275 17 2 regions region NNS 10_1101-2021_02_08_430275 17 3 linked link VBN 10_1101-2021_02_08_430275 17 4 to to IN 10_1101-2021_02_08_430275 17 5 the the DT 10_1101-2021_02_08_430275 17 6 trait-21 trait-21 NN 10_1101-2021_02_08_430275 17 7 controlling control VBG 10_1101-2021_02_08_430275 17 8 gene(s gene(s NNP 10_1101-2021_02_08_430275 17 9 ) ) -RRB- 10_1101-2021_02_08_430275 17 10 are be VBP 10_1101-2021_02_08_430275 17 11 then then RB 10_1101-2021_02_08_430275 17 12 identified identify VBN 10_1101-2021_02_08_430275 17 13 based base VBN 10_1101-2021_02_08_430275 17 14 on on IN 10_1101-2021_02_08_430275 17 15 the the DT 10_1101-2021_02_08_430275 17 16 enrichment22 enrichment22 NN 10_1101-2021_02_08_430275 17 17 of of IN 10_1101-2021_02_08_430275 17 18 the the DT 10_1101-2021_02_08_430275 17 19 SNP SNP NNP 10_1101-2021_02_08_430275 17 20 / / SYM 10_1101-2021_02_08_430275 17 21 InDel InDel NNP 10_1101-2021_02_08_430275 17 22 alleles allele NNS 10_1101-2021_02_08_430275 17 23 in in IN 10_1101-2021_02_08_430275 17 24 those those DT 10_1101-2021_02_08_430275 17 25 regions region NNS 10_1101-2021_02_08_430275 17 26 in in IN 10_1101-2021_02_08_430275 17 27 each each DT 10_1101-2021_02_08_430275 17 28 bulk bulk NN 10_1101-2021_02_08_430275 17 29 . . . 10_1101-2021_02_08_430275 18 1 The23 the23 NN 10_1101-2021_02_08_430275 18 2 time time NN 10_1101-2021_02_08_430275 18 3 - - HYPH 10_1101-2021_02_08_430275 18 4 consuming consume VBG 10_1101-2021_02_08_430275 18 5 and and CC 10_1101-2021_02_08_430275 18 6 labor labor NN 10_1101-2021_02_08_430275 18 7 - - HYPH 10_1101-2021_02_08_430275 18 8 intensive intensive JJ 10_1101-2021_02_08_430275 18 9 marker marker NN 10_1101-2021_02_08_430275 18 10 development development NN 10_1101-2021_02_08_430275 18 11 and24 and24 JJ 10_1101-2021_02_08_430275 18 12 genetic genetic JJ 10_1101-2021_02_08_430275 18 13 mapping mapping NN 10_1101-2021_02_08_430275 18 14 steps step NNS 10_1101-2021_02_08_430275 18 15 are be VBP 10_1101-2021_02_08_430275 18 16 eliminated eliminate VBN 10_1101-2021_02_08_430275 18 17 in in IN 10_1101-2021_02_08_430275 18 18 the the DT 10_1101-2021_02_08_430275 18 19 BSA BSA NNP 10_1101-2021_02_08_430275 18 20 - - HYPH 10_1101-2021_02_08_430275 18 21 Seq Seq NNP 10_1101-2021_02_08_430275 18 22 method.25 method.25 NNP 10_1101-2021_02_08_430275 18 23 Moreover moreover RB 10_1101-2021_02_08_430275 18 24 , , , 10_1101-2021_02_08_430275 18 25 SNPs SNPs NNPS 10_1101-2021_02_08_430275 18 26 / / SYM 10_1101-2021_02_08_430275 18 27 InDels indel NNS 10_1101-2021_02_08_430275 18 28 can can MD 10_1101-2021_02_08_430275 18 29 be be VB 10_1101-2021_02_08_430275 18 30 detected detect VBN 10_1101-2021_02_08_430275 18 31 genome genome NN 10_1101-2021_02_08_430275 18 32 - - HYPH 10_1101-2021_02_08_430275 18 33 wide wide JJ 10_1101-2021_02_08_430275 18 34 via via IN 10_1101-2021_02_08_430275 18 35 NGS,26 ngs,26 CD 10_1101-2021_02_08_430275 18 36 which which WDT 10_1101-2021_02_08_430275 18 37 allows allow VBZ 10_1101-2021_02_08_430275 18 38 for for IN 10_1101-2021_02_08_430275 18 39 the the DT 10_1101-2021_02_08_430275 18 40 reliable reliable JJ 10_1101-2021_02_08_430275 18 41 identification identification NN 10_1101-2021_02_08_430275 18 42 of of IN 10_1101-2021_02_08_430275 18 43 trait trait NN 10_1101-2021_02_08_430275 18 44 - - HYPH 10_1101-2021_02_08_430275 18 45 associated27 associated27 VBN 10_1101-2021_02_08_430275 18 46 genomic genomic JJ 10_1101-2021_02_08_430275 18 47 regions region NNS 10_1101-2021_02_08_430275 18 48 across across IN 10_1101-2021_02_08_430275 18 49 the the DT 10_1101-2021_02_08_430275 18 50 entire entire JJ 10_1101-2021_02_08_430275 18 51 genome.28 genome.28 NNP 10_1101-2021_02_08_430275 18 52 For for IN 10_1101-2021_02_08_430275 18 53 each each DT 10_1101-2021_02_08_430275 18 54 SNP SNP NNP 10_1101-2021_02_08_430275 18 55 / / SYM 10_1101-2021_02_08_430275 18 56 InDel InDel NNP 10_1101-2021_02_08_430275 18 57 in in IN 10_1101-2021_02_08_430275 18 58 a a DT 10_1101-2021_02_08_430275 18 59 BSA BSA NNP 10_1101-2021_02_08_430275 18 60 - - HYPH 10_1101-2021_02_08_430275 18 61 Seq Seq NNP 10_1101-2021_02_08_430275 18 62 dataset dataset NN 10_1101-2021_02_08_430275 18 63 , , , 10_1101-2021_02_08_430275 18 64 the the DT 10_1101-2021_02_08_430275 18 65 base base NN 10_1101-2021_02_08_430275 18 66 ( ( -LRB- 10_1101-2021_02_08_430275 18 67 or29 or29 NNP 10_1101-2021_02_08_430275 18 68 oligo oligo NNS 10_1101-2021_02_08_430275 18 69 in in IN 10_1101-2021_02_08_430275 18 70 the the DT 10_1101-2021_02_08_430275 18 71 case case NN 10_1101-2021_02_08_430275 18 72 of of IN 10_1101-2021_02_08_430275 18 73 an an DT 10_1101-2021_02_08_430275 18 74 InDel InDel NNP 10_1101-2021_02_08_430275 18 75 ) ) -RRB- 10_1101-2021_02_08_430275 18 76 that that DT 10_1101-2021_02_08_430275 18 77 is be VBZ 10_1101-2021_02_08_430275 18 78 the the DT 10_1101-2021_02_08_430275 18 79 same same JJ 10_1101-2021_02_08_430275 18 80 as as IN 10_1101-2021_02_08_430275 18 81 in in IN 10_1101-2021_02_08_430275 18 82 the the DT 10_1101-2021_02_08_430275 18 83 reference30 reference30 NNP 10_1101-2021_02_08_430275 18 84 genome genome NNP 10_1101-2021_02_08_430275 18 85 is be VBZ 10_1101-2021_02_08_430275 18 86 termed term VBN 10_1101-2021_02_08_430275 18 87 the the DT 10_1101-2021_02_08_430275 18 88 reference reference NN 10_1101-2021_02_08_430275 18 89 base base NN 10_1101-2021_02_08_430275 18 90 ( ( -LRB- 10_1101-2021_02_08_430275 18 91 REF REF NNP 10_1101-2021_02_08_430275 18 92 ) ) -RRB- 10_1101-2021_02_08_430275 18 93 , , , 10_1101-2021_02_08_430275 18 94 and and CC 10_1101-2021_02_08_430275 18 95 the the DT 10_1101-2021_02_08_430275 18 96 other31 other31 NNP 10_1101-2021_02_08_430275 18 97 base base NN 10_1101-2021_02_08_430275 18 98 is be VBZ 10_1101-2021_02_08_430275 18 99 termed term VBN 10_1101-2021_02_08_430275 18 100 the the DT 10_1101-2021_02_08_430275 18 101 alternative alternative JJ 10_1101-2021_02_08_430275 18 102 base base NN 10_1101-2021_02_08_430275 18 103 ( ( -LRB- 10_1101-2021_02_08_430275 18 104 ALT ALT NNP 10_1101-2021_02_08_430275 18 105 ) ) -RRB- 10_1101-2021_02_08_430275 18 106 . . . 10_1101-2021_02_08_430275 19 1 Because because IN 10_1101-2021_02_08_430275 19 2 each each DT 10_1101-2021_02_08_430275 19 3 bulk bulk NN 10_1101-2021_02_08_430275 19 4 32 32 CD 10_1101-2021_02_08_430275 19 5 contains contain VBZ 10_1101-2021_02_08_430275 19 6 many many JJ 10_1101-2021_02_08_430275 19 7 individuals individual NNS 10_1101-2021_02_08_430275 19 8 , , , 10_1101-2021_02_08_430275 19 9 the the DT 10_1101-2021_02_08_430275 19 10 vast vast JJ 10_1101-2021_02_08_430275 19 11 majority majority NN 10_1101-2021_02_08_430275 19 12 of of IN 10_1101-2021_02_08_430275 19 13 SNP SNP NNP 10_1101-2021_02_08_430275 19 14 loci loci NN 10_1101-2021_02_08_430275 19 15 in in IN 10_1101-2021_02_08_430275 19 16 33 33 CD 10_1101-2021_02_08_430275 19 17 the the DT 10_1101-2021_02_08_430275 19 18 dataset dataset NN 10_1101-2021_02_08_430275 19 19 have have VBP 10_1101-2021_02_08_430275 19 20 both both DT 10_1101-2021_02_08_430275 19 21 REF REF NNP 10_1101-2021_02_08_430275 19 22 and and CC 10_1101-2021_02_08_430275 19 23 ALT ALT NNP 10_1101-2021_02_08_430275 19 24 bases basis NNS 10_1101-2021_02_08_430275 19 25 . . . 10_1101-2021_02_08_430275 20 1 For for IN 10_1101-2021_02_08_430275 20 2 each each DT 10_1101-2021_02_08_430275 20 3 SNP SNP NNP 10_1101-2021_02_08_430275 20 4 , , , 10_1101-2021_02_08_430275 20 5 34 34 CD 10_1101-2021_02_08_430275 20 6 the the DT 10_1101-2021_02_08_430275 20 7 number number NN 10_1101-2021_02_08_430275 20 8 of of IN 10_1101-2021_02_08_430275 20 9 reads read NNS 10_1101-2021_02_08_430275 20 10 of of IN 10_1101-2021_02_08_430275 20 11 its -PRON- PRP$ 10_1101-2021_02_08_430275 20 12 REF REF NNP 10_1101-2021_02_08_430275 20 13 / / SYM 10_1101-2021_02_08_430275 20 14 ALT ALT NNP 10_1101-2021_02_08_430275 20 15 alleles allele NNS 10_1101-2021_02_08_430275 20 16 is be VBZ 10_1101-2021_02_08_430275 20 17 termed term VBN 10_1101-2021_02_08_430275 20 18 allele allele NNP 10_1101-2021_02_08_430275 20 19 35 35 CD 10_1101-2021_02_08_430275 20 20 depth depth NN 10_1101-2021_02_08_430275 20 21 ( ( -LRB- 10_1101-2021_02_08_430275 20 22 AD ad NN 10_1101-2021_02_08_430275 20 23 ) ) -RRB- 10_1101-2021_02_08_430275 20 24 . . . 10_1101-2021_02_08_430275 21 1 Because because IN 10_1101-2021_02_08_430275 21 2 of of IN 10_1101-2021_02_08_430275 21 3 the the DT 10_1101-2021_02_08_430275 21 4 phenotypic phenotypic NN 10_1101-2021_02_08_430275 21 5 selection selection NN 10_1101-2021_02_08_430275 21 6 via via IN 10_1101-2021_02_08_430275 21 7 bulking bulk VBG 10_1101-2021_02_08_430275 21 8 , , , 10_1101-2021_02_08_430275 21 9 36 36 CD 10_1101-2021_02_08_430275 21 10 for for IN 10_1101-2021_02_08_430275 21 11 trait trait NN 10_1101-2021_02_08_430275 21 12 - - HYPH 10_1101-2021_02_08_430275 21 13 associated associate VBN 10_1101-2021_02_08_430275 21 14 SNPs snp NNS 10_1101-2021_02_08_430275 21 15 , , , 10_1101-2021_02_08_430275 21 16 the the DT 10_1101-2021_02_08_430275 21 17 ALT ALT NNP 10_1101-2021_02_08_430275 21 18 allele allele NNS 10_1101-2021_02_08_430275 21 19 should should MD 10_1101-2021_02_08_430275 21 20 be be VB 10_1101-2021_02_08_430275 21 21 enriched enrich VBN 10_1101-2021_02_08_430275 21 22 37 37 CD 10_1101-2021_02_08_430275 21 23 in in IN 10_1101-2021_02_08_430275 21 24 one one CD 10_1101-2021_02_08_430275 21 25 bulk bulk NN 10_1101-2021_02_08_430275 21 26 while while IN 10_1101-2021_02_08_430275 21 27 the the DT 10_1101-2021_02_08_430275 21 28 REF REF NNP 10_1101-2021_02_08_430275 21 29 allele allele NNS 10_1101-2021_02_08_430275 21 30 should should MD 10_1101-2021_02_08_430275 21 31 be be VB 10_1101-2021_02_08_430275 21 32 enriched enrich VBN 10_1101-2021_02_08_430275 21 33 in in IN 10_1101-2021_02_08_430275 21 34 the the DT 10_1101-2021_02_08_430275 21 35 38 38 CD 10_1101-2021_02_08_430275 21 36 other other JJ 10_1101-2021_02_08_430275 21 37 . . . 10_1101-2021_02_08_430275 22 1 However however RB 10_1101-2021_02_08_430275 22 2 , , , 10_1101-2021_02_08_430275 22 3 for for IN 10_1101-2021_02_08_430275 22 4 SNPs snp NNS 10_1101-2021_02_08_430275 22 5 not not RB 10_1101-2021_02_08_430275 22 6 associated associate VBN 10_1101-2021_02_08_430275 22 7 with with IN 10_1101-2021_02_08_430275 22 8 the the DT 10_1101-2021_02_08_430275 22 9 trait trait NN 10_1101-2021_02_08_430275 22 10 , , , 10_1101-2021_02_08_430275 22 11 both both CC 10_1101-2021_02_08_430275 22 12 39 39 CD 10_1101-2021_02_08_430275 22 13 ALT ALT NNP 10_1101-2021_02_08_430275 22 14 and and CC 10_1101-2021_02_08_430275 22 15 REF REF NNP 10_1101-2021_02_08_430275 22 16 alleles allele NNS 10_1101-2021_02_08_430275 22 17 would would MD 10_1101-2021_02_08_430275 22 18 be be VB 10_1101-2021_02_08_430275 22 19 randomly randomly RB 10_1101-2021_02_08_430275 22 20 segregated segregate VBN 10_1101-2021_02_08_430275 22 21 in in IN 10_1101-2021_02_08_430275 22 22 both both DT 10_1101-2021_02_08_430275 22 23 40 40 CD 10_1101-2021_02_08_430275 22 24 bulks bulk NNS 10_1101-2021_02_08_430275 22 25 , , , 10_1101-2021_02_08_430275 22 26 and and CC 10_1101-2021_02_08_430275 22 27 neither neither DT 10_1101-2021_02_08_430275 22 28 enriched enrich VBN 10_1101-2021_02_08_430275 22 29 in in IN 10_1101-2021_02_08_430275 22 30 either either DT 10_1101-2021_02_08_430275 22 31 bulk bulk NN 10_1101-2021_02_08_430275 22 32 . . . 10_1101-2021_02_08_430275 23 1 Hence hence RB 10_1101-2021_02_08_430275 23 2 these these DT 10_1101-2021_02_08_430275 23 3 four four CD 10_1101-2021_02_08_430275 23 4 41 41 CD 10_1101-2021_02_08_430275 23 5 AD ad NN 10_1101-2021_02_08_430275 23 6 values value NNS 10_1101-2021_02_08_430275 23 7 can can MD 10_1101-2021_02_08_430275 23 8 be be VB 10_1101-2021_02_08_430275 23 9 used use VBN 10_1101-2021_02_08_430275 23 10 to to TO 10_1101-2021_02_08_430275 23 11 assess assess VB 10_1101-2021_02_08_430275 23 12 how how WRB 10_1101-2021_02_08_430275 23 13 likely likely RB 10_1101-2021_02_08_430275 23 14 a a DT 10_1101-2021_02_08_430275 23 15 SNP SNP NNP 10_1101-2021_02_08_430275 23 16 / / SYM 10_1101-2021_02_08_430275 23 17 InDel InDel NNP 10_1101-2021_02_08_430275 23 18 is be VBZ 10_1101-2021_02_08_430275 23 19 42 42 CD 10_1101-2021_02_08_430275 23 20 associated associate VBN 10_1101-2021_02_08_430275 23 21 with with IN 10_1101-2021_02_08_430275 23 22 the the DT 10_1101-2021_02_08_430275 23 23 trait trait NN 10_1101-2021_02_08_430275 23 24 . . . 10_1101-2021_02_08_430275 24 1 43 43 CD 10_1101-2021_02_08_430275 24 2 We -PRON- PRP 10_1101-2021_02_08_430275 24 3 have have VBP 10_1101-2021_02_08_430275 24 4 previously previously RB 10_1101-2021_02_08_430275 24 5 developed develop VBN 10_1101-2021_02_08_430275 24 6 the the DT 10_1101-2021_02_08_430275 24 7 significant significant JJ 10_1101-2021_02_08_430275 24 8 structural structural JJ 10_1101-2021_02_08_430275 24 9 44 44 CD 10_1101-2021_02_08_430275 24 10 variant variant JJ 10_1101-2021_02_08_430275 24 11 method method NN 10_1101-2021_02_08_430275 24 12 for for IN 10_1101-2021_02_08_430275 24 13 BSA BSA NNP 10_1101-2021_02_08_430275 24 14 - - HYPH 10_1101-2021_02_08_430275 24 15 Seq Seq NNP 10_1101-2021_02_08_430275 24 16 data data NN 10_1101-2021_02_08_430275 24 17 analysis analysis NN 10_1101-2021_02_08_430275 24 18 ( ( -LRB- 10_1101-2021_02_08_430275 24 19 8) 8) CD 10_1101-2021_02_08_430275 24 20 . . . 10_1101-2021_02_08_430275 25 1 In in IN 10_1101-2021_02_08_430275 25 2 this this DT 10_1101-2021_02_08_430275 25 3 method method NN 10_1101-2021_02_08_430275 25 4 , , , 10_1101-2021_02_08_430275 25 5 45 45 CD 10_1101-2021_02_08_430275 25 6 a a DT 10_1101-2021_02_08_430275 25 7 SNP SNP NNP 10_1101-2021_02_08_430275 25 8 / / SYM 10_1101-2021_02_08_430275 25 9 InDel InDel NNP 10_1101-2021_02_08_430275 25 10 is be VBZ 10_1101-2021_02_08_430275 25 11 assessed assess VBN 10_1101-2021_02_08_430275 25 12 with with IN 10_1101-2021_02_08_430275 25 13 Fisher Fisher NNP 10_1101-2021_02_08_430275 25 14 ’s ’s POS 10_1101-2021_02_08_430275 25 15 exact exact JJ 10_1101-2021_02_08_430275 25 16 test test NN 10_1101-2021_02_08_430275 25 17 using use VBG 10_1101-2021_02_08_430275 25 18 the the DT 10_1101-2021_02_08_430275 25 19 AD ad NN 10_1101-2021_02_08_430275 25 20 46 46 CD 10_1101-2021_02_08_430275 25 21 values value NNS 10_1101-2021_02_08_430275 25 22 of of IN 10_1101-2021_02_08_430275 25 23 both both DT 10_1101-2021_02_08_430275 25 24 bulks bulk NNS 10_1101-2021_02_08_430275 25 25 . . . 10_1101-2021_02_08_430275 26 1 A a DT 10_1101-2021_02_08_430275 26 2 SNP SNP NNP 10_1101-2021_02_08_430275 26 3 / / SYM 10_1101-2021_02_08_430275 26 4 InDel InDel NNP 10_1101-2021_02_08_430275 26 5 is be VBZ 10_1101-2021_02_08_430275 26 6 considered consider VBN 10_1101-2021_02_08_430275 26 7 significant significant JJ 10_1101-2021_02_08_430275 26 8 47 47 CD 10_1101-2021_02_08_430275 26 9 if if IN 10_1101-2021_02_08_430275 26 10 the the DT 10_1101-2021_02_08_430275 26 11 P p NN 10_1101-2021_02_08_430275 26 12 - - HYPH 10_1101-2021_02_08_430275 26 13 value value NN 10_1101-2021_02_08_430275 26 14 of of IN 10_1101-2021_02_08_430275 26 15 Fisher Fisher NNP 10_1101-2021_02_08_430275 26 16 ’s ’s POS 10_1101-2021_02_08_430275 26 17 exact exact JJ 10_1101-2021_02_08_430275 26 18 test test NN 10_1101-2021_02_08_430275 26 19 is be VBZ 10_1101-2021_02_08_430275 26 20 lower low JJR 10_1101-2021_02_08_430275 26 21 than than IN 10_1101-2021_02_08_430275 26 22 a a DT 10_1101-2021_02_08_430275 26 23 specific specific JJ 10_1101-2021_02_08_430275 26 24 48 48 CD 10_1101-2021_02_08_430275 26 25 cut cut VBN 10_1101-2021_02_08_430275 26 26 - - HYPH 10_1101-2021_02_08_430275 26 27 off off RP 10_1101-2021_02_08_430275 26 28 value value NN 10_1101-2021_02_08_430275 26 29 , , , 10_1101-2021_02_08_430275 26 30 e.g. e.g. RB 10_1101-2021_02_08_430275 26 31 , , , 10_1101-2021_02_08_430275 26 32 0.01 0.01 CD 10_1101-2021_02_08_430275 26 33 . . . 10_1101-2021_02_08_430275 27 1 A a DT 10_1101-2021_02_08_430275 27 2 genomic genomic JJ 10_1101-2021_02_08_430275 27 3 region region NN 10_1101-2021_02_08_430275 27 4 normally normally RB 10_1101-2021_02_08_430275 27 5 contains contain VBZ 10_1101-2021_02_08_430275 27 6 49 49 CD 10_1101-2021_02_08_430275 27 7 many many JJ 10_1101-2021_02_08_430275 27 8 SNPs snp NNS 10_1101-2021_02_08_430275 27 9 / / SYM 10_1101-2021_02_08_430275 27 10 InDels indel NNS 10_1101-2021_02_08_430275 27 11 . . . 10_1101-2021_02_08_430275 28 1 The the DT 10_1101-2021_02_08_430275 28 2 ratio ratio NN 10_1101-2021_02_08_430275 28 3 of of IN 10_1101-2021_02_08_430275 28 4 the the DT 10_1101-2021_02_08_430275 28 5 significant significant JJ 10_1101-2021_02_08_430275 28 6 structural structural JJ 10_1101-2021_02_08_430275 28 7 50 50 CD 10_1101-2021_02_08_430275 28 8 variants variant NNS 10_1101-2021_02_08_430275 28 9 to to IN 10_1101-2021_02_08_430275 28 10 the the DT 10_1101-2021_02_08_430275 28 11 total total JJ 10_1101-2021_02_08_430275 28 12 structural structural JJ 10_1101-2021_02_08_430275 28 13 variants variant NNS 10_1101-2021_02_08_430275 28 14 is be VBZ 10_1101-2021_02_08_430275 28 15 used use VBN 10_1101-2021_02_08_430275 28 16 to to TO 10_1101-2021_02_08_430275 28 17 judge judge VB 10_1101-2021_02_08_430275 28 18 if if IN 10_1101-2021_02_08_430275 28 19 51 51 CD 10_1101-2021_02_08_430275 28 20 this this DT 10_1101-2021_02_08_430275 28 21 genomic genomic JJ 10_1101-2021_02_08_430275 28 22 region region NN 10_1101-2021_02_08_430275 28 23 is be VBZ 10_1101-2021_02_08_430275 28 24 associated associate VBN 10_1101-2021_02_08_430275 28 25 with with IN 10_1101-2021_02_08_430275 28 26 the the DT 10_1101-2021_02_08_430275 28 27 trait trait NN 10_1101-2021_02_08_430275 28 28 of of IN 10_1101-2021_02_08_430275 28 29 interest interest NN 10_1101-2021_02_08_430275 28 30 . . . 10_1101-2021_02_08_430275 29 1 52 52 CD 10_1101-2021_02_08_430275 29 2 We -PRON- PRP 10_1101-2021_02_08_430275 29 3 tested test VBD 10_1101-2021_02_08_430275 29 4 this this DT 10_1101-2021_02_08_430275 29 5 method method NN 10_1101-2021_02_08_430275 29 6 using use VBG 10_1101-2021_02_08_430275 29 7 the the DT 10_1101-2021_02_08_430275 29 8 BSA BSA NNP 10_1101-2021_02_08_430275 29 9 - - HYPH 10_1101-2021_02_08_430275 29 10 Seq Seq NNP 10_1101-2021_02_08_430275 29 11 data datum NNS 10_1101-2021_02_08_430275 29 12 of of IN 10_1101-2021_02_08_430275 29 13 a a DT 10_1101-2021_02_08_430275 29 14 rice rice NN 10_1101-2021_02_08_430275 29 15 cold- cold- NN 10_1101-2021_02_08_430275 29 16 53 53 CD 10_1101-2021_02_08_430275 29 17 tolerance tolerance NN 10_1101-2021_02_08_430275 29 18 study study NN 10_1101-2021_02_08_430275 29 19 ( ( -LRB- 10_1101-2021_02_08_430275 29 20 9 9 CD 10_1101-2021_02_08_430275 29 21 ) ) -RRB- 10_1101-2021_02_08_430275 29 22 . . . 10_1101-2021_02_08_430275 30 1 One one CD 10_1101-2021_02_08_430275 30 2 of of IN 10_1101-2021_02_08_430275 30 3 the the DT 10_1101-2021_02_08_430275 30 4 parents parent NNS 10_1101-2021_02_08_430275 30 5 in in IN 10_1101-2021_02_08_430275 30 6 this this DT 10_1101-2021_02_08_430275 30 7 study study NN 10_1101-2021_02_08_430275 30 8 was be VBD 10_1101-2021_02_08_430275 30 9 rice rice NN 10_1101-2021_02_08_430275 30 10 54 54 CD 10_1101-2021_02_08_430275 30 11 cultivar cultivar JJ 10_1101-2021_02_08_430275 30 12 Oryza Oryza NNPS 10_1101-2021_02_08_430275 30 13 sativa sativa NN 10_1101-2021_02_08_430275 30 14 ssp ssp VB 10_1101-2021_02_08_430275 30 15 . . . 10_1101-2021_02_08_430275 31 1 japonica japonica NNP 10_1101-2021_02_08_430275 31 2 cv cv NNP 10_1101-2021_02_08_430275 31 3 . . . 10_1101-2021_02_08_430275 32 1 Nipponbare nipponbare NN 10_1101-2021_02_08_430275 32 2 . . . 10_1101-2021_02_08_430275 33 1 Its -PRON- PRP$ 10_1101-2021_02_08_430275 33 2 high- high- JJ 10_1101-2021_02_08_430275 33 3 55 55 CD 10_1101-2021_02_08_430275 33 4 quality quality NN 10_1101-2021_02_08_430275 33 5 assembled assemble VBN 10_1101-2021_02_08_430275 33 6 genome genome JJ 10_1101-2021_02_08_430275 33 7 sequences sequence NNS 10_1101-2021_02_08_430275 33 8 were be VBD 10_1101-2021_02_08_430275 33 9 used use VBN 10_1101-2021_02_08_430275 33 10 as as IN 10_1101-2021_02_08_430275 33 11 the the DT 10_1101-2021_02_08_430275 33 12 reference reference NN 10_1101-2021_02_08_430275 33 13 56 56 CD 10_1101-2021_02_08_430275 33 14 sequences sequence NNS 10_1101-2021_02_08_430275 33 15 for for IN 10_1101-2021_02_08_430275 33 16 SNP SNP NNP 10_1101-2021_02_08_430275 33 17 / / SYM 10_1101-2021_02_08_430275 33 18 InDel InDel NNP 10_1101-2021_02_08_430275 33 19 calling call VBG 10_1101-2021_02_08_430275 33 20 as as RB 10_1101-2021_02_08_430275 33 21 well well RB 10_1101-2021_02_08_430275 33 22 , , , 10_1101-2021_02_08_430275 33 23 which which WDT 10_1101-2021_02_08_430275 33 24 makes make VBZ 10_1101-2021_02_08_430275 33 25 the the DT 10_1101-2021_02_08_430275 33 26 57 57 CD 10_1101-2021_02_08_430275 33 27 genotype genotype NN 10_1101-2021_02_08_430275 33 28 calling calling NN 10_1101-2021_02_08_430275 33 29 and and CC 10_1101-2021_02_08_430275 33 30 SNP SNP NNP 10_1101-2021_02_08_430275 33 31 / / SYM 10_1101-2021_02_08_430275 33 32 InDel InDel NNP 10_1101-2021_02_08_430275 33 33 filtering filter VBG 10_1101-2021_02_08_430275 33 34 very very RB 10_1101-2021_02_08_430275 33 35 straightforward straightforward RB 10_1101-2021_02_08_430275 33 36 : : : 10_1101-2021_02_08_430275 33 37 58 58 CD 10_1101-2021_02_08_430275 33 38 any any DT 10_1101-2021_02_08_430275 33 39 locus locus NN 10_1101-2021_02_08_430275 33 40 in in IN 10_1101-2021_02_08_430275 33 41 any any DT 10_1101-2021_02_08_430275 33 42 bulk bulk NN 10_1101-2021_02_08_430275 33 43 that that WDT 10_1101-2021_02_08_430275 33 44 is be VBZ 10_1101-2021_02_08_430275 33 45 different different JJ 10_1101-2021_02_08_430275 33 46 from from IN 10_1101-2021_02_08_430275 33 47 the the DT 10_1101-2021_02_08_430275 33 48 REF REF NNP 10_1101-2021_02_08_430275 33 49 allele allele NNP 10_1101-2021_02_08_430275 33 50 is be VBZ 10_1101-2021_02_08_430275 33 51 59 59 CD 10_1101-2021_02_08_430275 33 52 a a DT 10_1101-2021_02_08_430275 33 53 valid valid JJ 10_1101-2021_02_08_430275 33 54 SNP SNP NNP 10_1101-2021_02_08_430275 33 55 / / SYM 10_1101-2021_02_08_430275 33 56 InDel InDel NNP 10_1101-2021_02_08_430275 33 57 ( ( -LRB- 10_1101-2021_02_08_430275 33 58 8) 8) CD 10_1101-2021_02_08_430275 33 59 . . . 10_1101-2021_02_08_430275 34 1 60 60 CD 10_1101-2021_02_08_430275 34 2 Only only JJ 10_1101-2021_02_08_430275 34 3 high high JJ 10_1101-2021_02_08_430275 34 4 - - HYPH 10_1101-2021_02_08_430275 34 5 quality quality NN 10_1101-2021_02_08_430275 34 6 assembled assemble VBN 10_1101-2021_02_08_430275 34 7 genome genome JJ 10_1101-2021_02_08_430275 34 8 sequences sequence NNS 10_1101-2021_02_08_430275 34 9 can can MD 10_1101-2021_02_08_430275 34 10 serve serve VB 10_1101-2021_02_08_430275 34 11 as as IN 10_1101-2021_02_08_430275 34 12 61 61 CD 10_1101-2021_02_08_430275 34 13 the the DT 10_1101-2021_02_08_430275 34 14 reference reference NN 10_1101-2021_02_08_430275 34 15 sequences sequence NNS 10_1101-2021_02_08_430275 34 16 in in IN 10_1101-2021_02_08_430275 34 17 genotype genotype NN 10_1101-2021_02_08_430275 34 18 calling calling NN 10_1101-2021_02_08_430275 34 19 , , , 10_1101-2021_02_08_430275 34 20 an an DT 10_1101-2021_02_08_430275 34 21 essential essential JJ 10_1101-2021_02_08_430275 34 22 step step NN 10_1101-2021_02_08_430275 34 23 62 62 CD 10_1101-2021_02_08_430275 34 24 in in IN 10_1101-2021_02_08_430275 34 25 BSA BSA NNP 10_1101-2021_02_08_430275 34 26 - - HYPH 10_1101-2021_02_08_430275 34 27 Seq Seq NNP 10_1101-2021_02_08_430275 34 28 data data NN 10_1101-2021_02_08_430275 34 29 analysis analysis NN 10_1101-2021_02_08_430275 34 30 . . . 10_1101-2021_02_08_430275 35 1 For for IN 10_1101-2021_02_08_430275 35 2 most most JJS 10_1101-2021_02_08_430275 35 3 species specie NNS 10_1101-2021_02_08_430275 35 4 , , , 10_1101-2021_02_08_430275 35 5 however however RB 10_1101-2021_02_08_430275 35 6 , , , 10_1101-2021_02_08_430275 35 7 such such JJ 10_1101-2021_02_08_430275 35 8 63 63 CD 10_1101-2021_02_08_430275 35 9 sequences sequence NNS 10_1101-2021_02_08_430275 35 10 are be VBP 10_1101-2021_02_08_430275 35 11 available available JJ 10_1101-2021_02_08_430275 35 12 for for IN 10_1101-2021_02_08_430275 35 13 only only RB 10_1101-2021_02_08_430275 35 14 a a DT 10_1101-2021_02_08_430275 35 15 single single JJ 10_1101-2021_02_08_430275 35 16 or or CC 10_1101-2021_02_08_430275 35 17 limited limited JJ 10_1101-2021_02_08_430275 35 18 number number NN 10_1101-2021_02_08_430275 35 19 of of IN 10_1101-2021_02_08_430275 35 20 64 64 CD 10_1101-2021_02_08_430275 35 21 lines line NNS 10_1101-2021_02_08_430275 35 22 . . . 10_1101-2021_02_08_430275 36 1 If if IN 10_1101-2021_02_08_430275 36 2 lines line NNS 10_1101-2021_02_08_430275 36 3 without without IN 10_1101-2021_02_08_430275 36 4 high high JJ 10_1101-2021_02_08_430275 36 5 - - HYPH 10_1101-2021_02_08_430275 36 6 quality quality NN 10_1101-2021_02_08_430275 36 7 assembled assemble VBN 10_1101-2021_02_08_430275 36 8 genome genome JJ 10_1101-2021_02_08_430275 36 9 sequences sequence NNS 10_1101-2021_02_08_430275 36 10 65 65 CD 10_1101-2021_02_08_430275 36 11 are be VBP 10_1101-2021_02_08_430275 36 12 used use VBN 10_1101-2021_02_08_430275 36 13 as as IN 10_1101-2021_02_08_430275 36 14 the the DT 10_1101-2021_02_08_430275 36 15 parents parent NNS 10_1101-2021_02_08_430275 36 16 in in IN 10_1101-2021_02_08_430275 36 17 BSA BSA NNP 10_1101-2021_02_08_430275 36 18 - - HYPH 10_1101-2021_02_08_430275 36 19 Seq Seq NNP 10_1101-2021_02_08_430275 36 20 studies study NNS 10_1101-2021_02_08_430275 36 21 , , , 10_1101-2021_02_08_430275 36 22 the the DT 10_1101-2021_02_08_430275 36 23 parental parental JJ 10_1101-2021_02_08_430275 36 24 66 66 CD 10_1101-2021_02_08_430275 36 25 genomes genome NNS 10_1101-2021_02_08_430275 36 26 are be VBP 10_1101-2021_02_08_430275 36 27 often often RB 10_1101-2021_02_08_430275 36 28 sequenced sequence VBN 10_1101-2021_02_08_430275 36 29 via via IN 10_1101-2021_02_08_430275 36 30 NGS NGS NNP 10_1101-2021_02_08_430275 36 31 for for IN 10_1101-2021_02_08_430275 36 32 the the DT 10_1101-2021_02_08_430275 36 33 determination determination NN 10_1101-2021_02_08_430275 36 34 67 67 CD 10_1101-2021_02_08_430275 36 35 Significance Significance NNP 10_1101-2021_02_08_430275 36 36 Statement Statement NNP 10_1101-2021_02_08_430275 36 37 BSA BSA NNP 10_1101-2021_02_08_430275 36 38 - - HYPH 10_1101-2021_02_08_430275 36 39 Seq Seq NNP 10_1101-2021_02_08_430275 36 40 can can MD 10_1101-2021_02_08_430275 36 41 be be VB 10_1101-2021_02_08_430275 36 42 utilized utilize VBN 10_1101-2021_02_08_430275 36 43 to to TO 10_1101-2021_02_08_430275 36 44 rapidly rapidly RB 10_1101-2021_02_08_430275 36 45 identify identify VB 10_1101-2021_02_08_430275 36 46 structural structural JJ 10_1101-2021_02_08_430275 36 47 variant- variant- NNP 10_1101-2021_02_08_430275 36 48 trait trait NNP 10_1101-2021_02_08_430275 36 49 associations association NNS 10_1101-2021_02_08_430275 36 50 , , , 10_1101-2021_02_08_430275 36 51 and and CC 10_1101-2021_02_08_430275 36 52 our -PRON- PRP$ 10_1101-2021_02_08_430275 36 53 modified modified JJ 10_1101-2021_02_08_430275 36 54 significant significant JJ 10_1101-2021_02_08_430275 36 55 structural structural JJ 10_1101-2021_02_08_430275 36 56 variant variant JJ 10_1101-2021_02_08_430275 36 57 method method NN 10_1101-2021_02_08_430275 36 58 allows allow VBZ 10_1101-2021_02_08_430275 36 59 the the DT 10_1101-2021_02_08_430275 36 60 detection detection NN 10_1101-2021_02_08_430275 36 61 of of IN 10_1101-2021_02_08_430275 36 62 such such JJ 10_1101-2021_02_08_430275 36 63 associations association NNS 10_1101-2021_02_08_430275 36 64 without without IN 10_1101-2021_02_08_430275 36 65 se- se- NN 10_1101-2021_02_08_430275 36 66 quencing quence VBG 10_1101-2021_02_08_430275 36 67 the the DT 10_1101-2021_02_08_430275 36 68 parental parental JJ 10_1101-2021_02_08_430275 36 69 genomes genome NNS 10_1101-2021_02_08_430275 36 70 , , , 10_1101-2021_02_08_430275 36 71 leading lead VBG 10_1101-2021_02_08_430275 36 72 to to IN 10_1101-2021_02_08_430275 36 73 further further RB 10_1101-2021_02_08_430275 36 74 lower lower VB 10_1101-2021_02_08_430275 36 75 the the DT 10_1101-2021_02_08_430275 36 76 sequencing sequencing NN 10_1101-2021_02_08_430275 36 77 cost cost NN 10_1101-2021_02_08_430275 36 78 and and CC 10_1101-2021_02_08_430275 36 79 making make VBG 10_1101-2021_02_08_430275 36 80 BSA BSA NNP 10_1101-2021_02_08_430275 36 81 - - HYPH 10_1101-2021_02_08_430275 36 82 Seq seq NN 10_1101-2021_02_08_430275 36 83 more more RBR 10_1101-2021_02_08_430275 36 84 accessible accessible JJ 10_1101-2021_02_08_430275 36 85 to to IN 10_1101-2021_02_08_430275 36 86 the the DT 10_1101-2021_02_08_430275 36 87 research research NN 10_1101-2021_02_08_430275 36 88 community community NN 10_1101-2021_02_08_430275 36 89 and and CC 10_1101-2021_02_08_430275 36 90 more more RBR 10_1101-2021_02_08_430275 36 91 applicable applicable JJ 10_1101-2021_02_08_430275 36 92 to to IN 10_1101-2021_02_08_430275 36 93 the the DT 10_1101-2021_02_08_430275 36 94 species specie NNS 10_1101-2021_02_08_430275 36 95 with with IN 10_1101-2021_02_08_430275 36 96 a a DT 10_1101-2021_02_08_430275 36 97 large large JJ 10_1101-2021_02_08_430275 36 98 genome genome NN 10_1101-2021_02_08_430275 36 99 . . . 10_1101-2021_02_08_430275 37 1 Author author NN 10_1101-2021_02_08_430275 37 2 contributions contribution NNS 10_1101-2021_02_08_430275 37 3 : : : 10_1101-2021_02_08_430275 37 4 JZ JZ NNP 10_1101-2021_02_08_430275 37 5 and and CC 10_1101-2021_02_08_430275 37 6 DRP DRP NNP 10_1101-2021_02_08_430275 37 7 conceived conceive VBD 10_1101-2021_02_08_430275 37 8 the the DT 10_1101-2021_02_08_430275 37 9 study study NN 10_1101-2021_02_08_430275 37 10 . . . 10_1101-2021_02_08_430275 38 1 JZ JZ NNP 10_1101-2021_02_08_430275 38 2 developed develop VBD 10_1101-2021_02_08_430275 38 3 the the DT 10_1101-2021_02_08_430275 38 4 algorithm algorithm NNP 10_1101-2021_02_08_430275 38 5 , , , 10_1101-2021_02_08_430275 38 6 wrote write VBD 10_1101-2021_02_08_430275 38 7 the the DT 10_1101-2021_02_08_430275 38 8 Python Python NNP 10_1101-2021_02_08_430275 38 9 code code NN 10_1101-2021_02_08_430275 38 10 , , , 10_1101-2021_02_08_430275 38 11 analyzed analyze VBD 10_1101-2021_02_08_430275 38 12 the the DT 10_1101-2021_02_08_430275 38 13 data datum NNS 10_1101-2021_02_08_430275 38 14 , , , 10_1101-2021_02_08_430275 38 15 and and CC 10_1101-2021_02_08_430275 38 16 wrote write VBD 10_1101-2021_02_08_430275 38 17 and and CC 10_1101-2021_02_08_430275 38 18 edited edit VBD 10_1101-2021_02_08_430275 38 19 the the DT 10_1101-2021_02_08_430275 38 20 manuscript manuscript NN 10_1101-2021_02_08_430275 38 21 . . . 10_1101-2021_02_08_430275 39 1 DRP DRP NNP 10_1101-2021_02_08_430275 39 2 edited edit VBD 10_1101-2021_02_08_430275 39 3 the the DT 10_1101-2021_02_08_430275 39 4 manuscript manuscript NN 10_1101-2021_02_08_430275 39 5 and and CC 10_1101-2021_02_08_430275 39 6 supervised supervise VBD 10_1101-2021_02_08_430275 39 7 the the DT 10_1101-2021_02_08_430275 39 8 project project NN 10_1101-2021_02_08_430275 39 9 . . . 10_1101-2021_02_08_430275 40 1 The the DT 10_1101-2021_02_08_430275 40 2 authors author NNS 10_1101-2021_02_08_430275 40 3 declare declare VBP 10_1101-2021_02_08_430275 40 4 no no DT 10_1101-2021_02_08_430275 40 5 conflict conflict NN 10_1101-2021_02_08_430275 40 6 of of IN 10_1101-2021_02_08_430275 40 7 interest interest NN 10_1101-2021_02_08_430275 40 8 . . . 10_1101-2021_02_08_430275 41 1 � � , 10_1101-2021_02_08_430275 41 2 To to IN 10_1101-2021_02_08_430275 41 3 whom whom WP 10_1101-2021_02_08_430275 41 4 correspondence correspondence NN 10_1101-2021_02_08_430275 41 5 should should MD 10_1101-2021_02_08_430275 41 6 be be VB 10_1101-2021_02_08_430275 41 7 addressed address VBN 10_1101-2021_02_08_430275 41 8 . . . 10_1101-2021_02_08_430275 42 1 E e NN 10_1101-2021_02_08_430275 42 2 - - NN 10_1101-2021_02_08_430275 42 3 mail mail NN 10_1101-2021_02_08_430275 42 4 : : : 10_1101-2021_02_08_430275 42 5 dilip_panthee@ncsu.edu dilip_panthee@ncsu.edu NNS 10_1101-2021_02_08_430275 42 6 or or CC 10_1101-2021_02_08_430275 42 7 zhang.jianbo@gmail.com zhang.jianbo@gmail.com ADD 10_1101-2021_02_08_430275 42 8 https://doi.org/10.1101/654137 https://doi.org/10.1101/654137 NNP 10_1101-2021_02_08_430275 42 9 bioRχiv bioRχiv NNP 10_1101-2021_02_08_430275 42 10 | | NNP 10_1101-2021_02_08_430275 42 11 November November NNP 10_1101-2021_02_08_430275 42 12 24 24 CD 10_1101-2021_02_08_430275 42 13 , , , 10_1101-2021_02_08_430275 42 14 2020 2020 CD 10_1101-2021_02_08_430275 42 15 | | SYM 10_1101-2021_02_08_430275 42 16 1 1 CD 10_1101-2021_02_08_430275 42 17 .CC .CC : 10_1101-2021_02_08_430275 42 18 - - HYPH 10_1101-2021_02_08_430275 42 19 BY by IN 10_1101-2021_02_08_430275 42 20 - - HYPH 10_1101-2021_02_08_430275 42 21 NC NC NNP 10_1101-2021_02_08_430275 42 22 - - HYPH 10_1101-2021_02_08_430275 42 23 ND ND NNP 10_1101-2021_02_08_430275 42 24 4.0 4.0 CD 10_1101-2021_02_08_430275 42 25 International International NNP 10_1101-2021_02_08_430275 42 26 licenseavailable licenseavailable NN 10_1101-2021_02_08_430275 42 27 under under IN 10_1101-2021_02_08_430275 42 28 a a DT 10_1101-2021_02_08_430275 42 29 ( ( -LRB- 10_1101-2021_02_08_430275 42 30 which which WDT 10_1101-2021_02_08_430275 42 31 was be VBD 10_1101-2021_02_08_430275 42 32 not not RB 10_1101-2021_02_08_430275 42 33 certified certify VBN 10_1101-2021_02_08_430275 42 34 by by IN 10_1101-2021_02_08_430275 42 35 peer peer NN 10_1101-2021_02_08_430275 42 36 review review NN 10_1101-2021_02_08_430275 42 37 ) ) -RRB- 10_1101-2021_02_08_430275 42 38 is be VBZ 10_1101-2021_02_08_430275 42 39 the the DT 10_1101-2021_02_08_430275 42 40 author author NN 10_1101-2021_02_08_430275 42 41 / / SYM 10_1101-2021_02_08_430275 42 42 funder funder NN 10_1101-2021_02_08_430275 42 43 , , , 10_1101-2021_02_08_430275 42 44 who who WP 10_1101-2021_02_08_430275 42 45 has have VBZ 10_1101-2021_02_08_430275 42 46 granted grant VBN 10_1101-2021_02_08_430275 42 47 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 42 48 a a DT 10_1101-2021_02_08_430275 42 49 license license NN 10_1101-2021_02_08_430275 42 50 to to TO 10_1101-2021_02_08_430275 42 51 display display VB 10_1101-2021_02_08_430275 42 52 the the DT 10_1101-2021_02_08_430275 42 53 preprint preprint NN 10_1101-2021_02_08_430275 42 54 in in IN 10_1101-2021_02_08_430275 42 55 perpetuity perpetuity NN 10_1101-2021_02_08_430275 42 56 . . . 10_1101-2021_02_08_430275 43 1 It -PRON- PRP 10_1101-2021_02_08_430275 43 2 is be VBZ 10_1101-2021_02_08_430275 43 3 made make VBN 10_1101-2021_02_08_430275 43 4 The the DT 10_1101-2021_02_08_430275 43 5 copyright copyright NN 10_1101-2021_02_08_430275 43 6 holder holder NN 10_1101-2021_02_08_430275 43 7 for for IN 10_1101-2021_02_08_430275 43 8 this this DT 10_1101-2021_02_08_430275 43 9 preprintthis preprintthis NN 10_1101-2021_02_08_430275 43 10 version version NN 10_1101-2021_02_08_430275 43 11 posted post VBD 10_1101-2021_02_08_430275 43 12 February February NNP 10_1101-2021_02_08_430275 43 13 10 10 CD 10_1101-2021_02_08_430275 43 14 , , , 10_1101-2021_02_08_430275 43 15 2021 2021 CD 10_1101-2021_02_08_430275 43 16 . . . 10_1101-2021_02_08_430275 43 17 ; ; : 10_1101-2021_02_08_430275 43 18 https://doi.org/10.1101/2021.02.08.430275doi https://doi.org/10.1101/2021.02.08.430275doi ADD 10_1101-2021_02_08_430275 43 19 : : : 10_1101-2021_02_08_430275 43 20 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 43 21 preprint preprint NN 10_1101-2021_02_08_430275 43 22 https://doi.org/10.1101/654137 https://doi.org/10.1101/654137 VBZ 10_1101-2021_02_08_430275 43 23 https://doi.org/10.1101/2021.02.08.430275 https://doi.org/10.1101/2021.02.08.430275 NNP 10_1101-2021_02_08_430275 43 24 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_02_08_430275 43 25 DR DR NNP 10_1101-2021_02_08_430275 43 26 AF AF NNP 10_1101-2021_02_08_430275 43 27 T T NNP 10_1101-2021_02_08_430275 43 28 of of IN 10_1101-2021_02_08_430275 43 29 the the DT 10_1101-2021_02_08_430275 43 30 parental parental JJ 10_1101-2021_02_08_430275 43 31 origin origin NN 10_1101-2021_02_08_430275 43 32 of of IN 10_1101-2021_02_08_430275 43 33 SNP SNP NNP 10_1101-2021_02_08_430275 43 34 alleles allele NNS 10_1101-2021_02_08_430275 43 35 and and CC 10_1101-2021_02_08_430275 43 36 the the DT 10_1101-2021_02_08_430275 43 37 identification68 identification68 NNS 10_1101-2021_02_08_430275 43 38 of of IN 10_1101-2021_02_08_430275 43 39 parental parental JJ 10_1101-2021_02_08_430275 43 40 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 43 41 SNPs snp NNS 10_1101-2021_02_08_430275 43 42 . . . 10_1101-2021_02_08_430275 44 1 Modification modification NN 10_1101-2021_02_08_430275 44 2 of of IN 10_1101-2021_02_08_430275 44 3 our -PRON- PRP$ 10_1101-2021_02_08_430275 44 4 original69 original69 NN 10_1101-2021_02_08_430275 44 5 method method NN 10_1101-2021_02_08_430275 44 6 to to TO 10_1101-2021_02_08_430275 44 7 allow allow VB 10_1101-2021_02_08_430275 44 8 the the DT 10_1101-2021_02_08_430275 44 9 analysis analysis NN 10_1101-2021_02_08_430275 44 10 of of IN 10_1101-2021_02_08_430275 44 11 BSA BSA NNP 10_1101-2021_02_08_430275 44 12 - - HYPH 10_1101-2021_02_08_430275 44 13 Seq Seq NNP 10_1101-2021_02_08_430275 44 14 data datum NNS 10_1101-2021_02_08_430275 44 15 in in IN 10_1101-2021_02_08_430275 44 16 the the DT 10_1101-2021_02_08_430275 44 17 absence absence NN 10_1101-2021_02_08_430275 44 18 of70 of70 NNS 10_1101-2021_02_08_430275 44 19 assembled assemble VBN 10_1101-2021_02_08_430275 44 20 or or CC 10_1101-2021_02_08_430275 44 21 NGS NGS NNP 10_1101-2021_02_08_430275 44 22 - - HYPH 10_1101-2021_02_08_430275 44 23 generated generate VBN 10_1101-2021_02_08_430275 44 24 parental parental JJ 10_1101-2021_02_08_430275 44 25 genome genome NN 10_1101-2021_02_08_430275 44 26 sequences sequence NNS 10_1101-2021_02_08_430275 44 27 would71 would71 JJS 10_1101-2021_02_08_430275 44 28 provide provide VBP 10_1101-2021_02_08_430275 44 29 greater great JJR 10_1101-2021_02_08_430275 44 30 flexibility flexibility NN 10_1101-2021_02_08_430275 44 31 and and CC 10_1101-2021_02_08_430275 44 32 significantly significantly RB 10_1101-2021_02_08_430275 44 33 reduce reduce VB 10_1101-2021_02_08_430275 44 34 sequencing72 sequencing72 NNP 10_1101-2021_02_08_430275 44 35 costs cost NNS 10_1101-2021_02_08_430275 44 36 . . . 10_1101-2021_02_08_430275 45 1 Hence hence RB 10_1101-2021_02_08_430275 45 2 , , , 10_1101-2021_02_08_430275 45 3 we -PRON- PRP 10_1101-2021_02_08_430275 45 4 modified modify VBD 10_1101-2021_02_08_430275 45 5 our -PRON- PRP$ 10_1101-2021_02_08_430275 45 6 original original JJ 10_1101-2021_02_08_430275 45 7 script script NN 10_1101-2021_02_08_430275 45 8 to to TO 10_1101-2021_02_08_430275 45 9 allow allow VB 10_1101-2021_02_08_430275 45 10 for73 for73 JJR 10_1101-2021_02_08_430275 45 11 the the DT 10_1101-2021_02_08_430275 45 12 identification identification NN 10_1101-2021_02_08_430275 45 13 of of IN 10_1101-2021_02_08_430275 45 14 the the DT 10_1101-2021_02_08_430275 45 15 false false JJ 10_1101-2021_02_08_430275 45 16 - - HYPH 10_1101-2021_02_08_430275 45 17 positive positive JJ 10_1101-2021_02_08_430275 45 18 SNPs snp NNS 10_1101-2021_02_08_430275 45 19 / / SYM 10_1101-2021_02_08_430275 45 20 InDels indel NNS 10_1101-2021_02_08_430275 45 21 and and CC 10_1101-2021_02_08_430275 45 22 part74 part74 NNP 10_1101-2021_02_08_430275 45 23 of of IN 10_1101-2021_02_08_430275 45 24 the the DT 10_1101-2021_02_08_430275 45 25 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 45 26 loci loci NN 10_1101-2021_02_08_430275 45 27 in in IN 10_1101-2021_02_08_430275 45 28 the the DT 10_1101-2021_02_08_430275 45 29 parents parent NNS 10_1101-2021_02_08_430275 45 30 without without IN 10_1101-2021_02_08_430275 45 31 the the DT 10_1101-2021_02_08_430275 45 32 aid aid NN 10_1101-2021_02_08_430275 45 33 of75 of75 NNP 10_1101-2021_02_08_430275 45 34 the the DT 10_1101-2021_02_08_430275 45 35 parental parental JJ 10_1101-2021_02_08_430275 45 36 genome genome JJ 10_1101-2021_02_08_430275 45 37 sequences sequence NNS 10_1101-2021_02_08_430275 45 38 . . . 10_1101-2021_02_08_430275 46 1 Using use VBG 10_1101-2021_02_08_430275 46 2 the the DT 10_1101-2021_02_08_430275 46 3 modified modify VBN 10_1101-2021_02_08_430275 46 4 script,76 script,76 NN 10_1101-2021_02_08_430275 46 5 along along IN 10_1101-2021_02_08_430275 46 6 with with IN 10_1101-2021_02_08_430275 46 7 the the DT 10_1101-2021_02_08_430275 46 8 scripts script NNS 10_1101-2021_02_08_430275 46 9 for for IN 10_1101-2021_02_08_430275 46 10 the the DT 10_1101-2021_02_08_430275 46 11 standard standard JJ 10_1101-2021_02_08_430275 46 12 G g NN 10_1101-2021_02_08_430275 46 13 - - HYPH 10_1101-2021_02_08_430275 46 14 statistic statistic NNP 10_1101-2021_02_08_430275 46 15 and and CC 10_1101-2021_02_08_430275 46 16 allele77 allele77 NNP 10_1101-2021_02_08_430275 46 17 frequency frequency NN 10_1101-2021_02_08_430275 46 18 methods method NNS 10_1101-2021_02_08_430275 46 19 ( ( -LRB- 10_1101-2021_02_08_430275 46 20 10 10 CD 10_1101-2021_02_08_430275 46 21 , , , 10_1101-2021_02_08_430275 46 22 11 11 CD 10_1101-2021_02_08_430275 46 23 ) ) -RRB- 10_1101-2021_02_08_430275 46 24 , , , 10_1101-2021_02_08_430275 46 25 we -PRON- PRP 10_1101-2021_02_08_430275 46 26 analyzed analyze VBD 10_1101-2021_02_08_430275 46 27 a a DT 10_1101-2021_02_08_430275 46 28 public public JJ 10_1101-2021_02_08_430275 46 29 BSA BSA NNP 10_1101-2021_02_08_430275 46 30 - - HYPH 10_1101-2021_02_08_430275 46 31 Seq78 Seq78 NNP 10_1101-2021_02_08_430275 46 32 dataset dataset NN 10_1101-2021_02_08_430275 46 33 using use VBG 10_1101-2021_02_08_430275 46 34 either either CC 10_1101-2021_02_08_430275 46 35 the the DT 10_1101-2021_02_08_430275 46 36 genome genome JJ 10_1101-2021_02_08_430275 46 37 sequences sequence NNS 10_1101-2021_02_08_430275 46 38 of of IN 10_1101-2021_02_08_430275 46 39 both both CC 10_1101-2021_02_08_430275 46 40 the the DT 10_1101-2021_02_08_430275 46 41 parents79 parents79 NNPS 10_1101-2021_02_08_430275 46 42 and and CC 10_1101-2021_02_08_430275 46 43 the the DT 10_1101-2021_02_08_430275 46 44 bulks bulk NNS 10_1101-2021_02_08_430275 46 45 , , , 10_1101-2021_02_08_430275 46 46 or or CC 10_1101-2021_02_08_430275 46 47 the the DT 10_1101-2021_02_08_430275 46 48 bulk bulk JJ 10_1101-2021_02_08_430275 46 49 genome genome JJ 10_1101-2021_02_08_430275 46 50 sequences sequence NNS 10_1101-2021_02_08_430275 46 51 alone alone RB 10_1101-2021_02_08_430275 46 52 . . . 10_1101-2021_02_08_430275 47 1 The80 the80 NN 10_1101-2021_02_08_430275 47 2 results result NNS 10_1101-2021_02_08_430275 47 3 revealed reveal VBD 10_1101-2021_02_08_430275 47 4 that that IN 10_1101-2021_02_08_430275 47 5 reliable reliable JJ 10_1101-2021_02_08_430275 47 6 detection detection NN 10_1101-2021_02_08_430275 47 7 of of IN 10_1101-2021_02_08_430275 47 8 genomic genomic JJ 10_1101-2021_02_08_430275 47 9 region region NN 10_1101-2021_02_08_430275 47 10 - - HYPH 10_1101-2021_02_08_430275 47 11 trait81 trait81 NN 10_1101-2021_02_08_430275 47 12 associations association NNS 10_1101-2021_02_08_430275 47 13 can can MD 10_1101-2021_02_08_430275 47 14 be be VB 10_1101-2021_02_08_430275 47 15 achieved achieve VBN 10_1101-2021_02_08_430275 47 16 only only RB 10_1101-2021_02_08_430275 47 17 via via IN 10_1101-2021_02_08_430275 47 18 our -PRON- PRP$ 10_1101-2021_02_08_430275 47 19 modified modify VBN 10_1101-2021_02_08_430275 47 20 script script NN 10_1101-2021_02_08_430275 47 21 when82 when82 NNPS 10_1101-2021_02_08_430275 47 22 using use VBG 10_1101-2021_02_08_430275 47 23 only only RB 10_1101-2021_02_08_430275 47 24 the the DT 10_1101-2021_02_08_430275 47 25 bulk bulk NN 10_1101-2021_02_08_430275 47 26 genome genome JJ 10_1101-2021_02_08_430275 47 27 sequences.83 sequences.83 NNP 10_1101-2021_02_08_430275 47 28 Materials Materials NNP 10_1101-2021_02_08_430275 47 29 and and CC 10_1101-2021_02_08_430275 47 30 Methods84 Methods84 NNP 10_1101-2021_02_08_430275 47 31 The the DT 10_1101-2021_02_08_430275 47 32 sequencing sequence VBG 10_1101-2021_02_08_430275 47 33 data datum NNS 10_1101-2021_02_08_430275 47 34 used use VBN 10_1101-2021_02_08_430275 47 35 in in IN 10_1101-2021_02_08_430275 47 36 this this DT 10_1101-2021_02_08_430275 47 37 study study NN 10_1101-2021_02_08_430275 47 38 were be VBD 10_1101-2021_02_08_430275 47 39 generated generate VBN 10_1101-2021_02_08_430275 47 40 by by IN 10_1101-2021_02_08_430275 47 41 Lahari Lahari NNP 10_1101-2021_02_08_430275 47 42 et85 et85 NNP 10_1101-2021_02_08_430275 47 43 al al NNP 10_1101-2021_02_08_430275 47 44 . . . 10_1101-2021_02_08_430275 48 1 ( ( -LRB- 10_1101-2021_02_08_430275 48 2 12 12 CD 10_1101-2021_02_08_430275 48 3 ) ) -RRB- 10_1101-2021_02_08_430275 48 4 . . . 10_1101-2021_02_08_430275 49 1 Using use VBG 10_1101-2021_02_08_430275 49 2 the the DT 10_1101-2021_02_08_430275 49 3 allele allele NNP 10_1101-2021_02_08_430275 49 4 frequency frequency NN 10_1101-2021_02_08_430275 49 5 method method NN 10_1101-2021_02_08_430275 49 6 , , , 10_1101-2021_02_08_430275 49 7 the the DT 10_1101-2021_02_08_430275 49 8 authors author NNS 10_1101-2021_02_08_430275 49 9 identified86 identified86 VBZ 10_1101-2021_02_08_430275 49 10 a a DT 10_1101-2021_02_08_430275 49 11 single single JJ 10_1101-2021_02_08_430275 49 12 locus locus NN 10_1101-2021_02_08_430275 49 13 for for IN 10_1101-2021_02_08_430275 49 14 root root NN 10_1101-2021_02_08_430275 49 15 - - HYPH 10_1101-2021_02_08_430275 49 16 knot knot NN 10_1101-2021_02_08_430275 49 17 nematode nematode NNP 10_1101-2021_02_08_430275 49 18 resistance resistance NNP 10_1101-2021_02_08_430275 49 19 in in IN 10_1101-2021_02_08_430275 49 20 rice rice NN 10_1101-2021_02_08_430275 49 21 . . . 10_1101-2021_02_08_430275 50 1 In in IN 10_1101-2021_02_08_430275 50 2 that87 that87 NNP 10_1101-2021_02_08_430275 50 3 study study NN 10_1101-2021_02_08_430275 50 4 , , , 10_1101-2021_02_08_430275 50 5 the the DT 10_1101-2021_02_08_430275 50 6 parents parent NNS 10_1101-2021_02_08_430275 50 7 of of IN 10_1101-2021_02_08_430275 50 8 the the DT 10_1101-2021_02_08_430275 50 9 F2 f2 NN 10_1101-2021_02_08_430275 50 10 population population NN 10_1101-2021_02_08_430275 50 11 were be VBD 10_1101-2021_02_08_430275 50 12 LD24 LD24 NNP 10_1101-2021_02_08_430275 50 13 and and CC 10_1101-2021_02_08_430275 50 14 VialoneNano,88 VialoneNano,88 NNP 10_1101-2021_02_08_430275 50 15 yielding yield VBG 10_1101-2021_02_08_430275 50 16 an an DT 10_1101-2021_02_08_430275 50 17 F2 f2 NN 10_1101-2021_02_08_430275 50 18 population population NN 10_1101-2021_02_08_430275 50 19 size size NN 10_1101-2021_02_08_430275 50 20 of of IN 10_1101-2021_02_08_430275 50 21 178 178 CD 10_1101-2021_02_08_430275 50 22 ( ( -LRB- 10_1101-2021_02_08_430275 50 23 plants plant NNS 10_1101-2021_02_08_430275 50 24 ) ) -RRB- 10_1101-2021_02_08_430275 50 25 , , , 10_1101-2021_02_08_430275 50 26 and and CC 10_1101-2021_02_08_430275 50 27 both both CC 10_1101-2021_02_08_430275 50 28 the the DT 10_1101-2021_02_08_430275 50 29 resistant89 resistant89 NNP 10_1101-2021_02_08_430275 50 30 bulk bulk NN 10_1101-2021_02_08_430275 50 31 and and CC 10_1101-2021_02_08_430275 50 32 the the DT 10_1101-2021_02_08_430275 50 33 susceptible susceptible JJ 10_1101-2021_02_08_430275 50 34 bulk bulk NN 10_1101-2021_02_08_430275 50 35 contained contain VBD 10_1101-2021_02_08_430275 50 36 23 23 CD 10_1101-2021_02_08_430275 50 37 plants plant NNS 10_1101-2021_02_08_430275 50 38 each each DT 10_1101-2021_02_08_430275 50 39 . . . 10_1101-2021_02_08_430275 51 1 The the DT 10_1101-2021_02_08_430275 51 2 DNA90 dna90 JJ 10_1101-2021_02_08_430275 51 3 samples sample NNS 10_1101-2021_02_08_430275 51 4 of of IN 10_1101-2021_02_08_430275 51 5 both both PDT 10_1101-2021_02_08_430275 51 6 the the DT 10_1101-2021_02_08_430275 51 7 parents parent NNS 10_1101-2021_02_08_430275 51 8 and and CC 10_1101-2021_02_08_430275 51 9 the the DT 10_1101-2021_02_08_430275 51 10 bulks bulk NNS 10_1101-2021_02_08_430275 51 11 were be VBD 10_1101-2021_02_08_430275 51 12 sequenced sequence VBN 10_1101-2021_02_08_430275 51 13 using91 using91 NNP 10_1101-2021_02_08_430275 51 14 Illumina Illumina NNP 10_1101-2021_02_08_430275 51 15 MiSeq MiSeq NNP 10_1101-2021_02_08_430275 51 16 Sequencing Sequencing NNP 10_1101-2021_02_08_430275 51 17 System System NNP 10_1101-2021_02_08_430275 51 18 and and CC 10_1101-2021_02_08_430275 51 19 MiSeq MiSeq NNP 10_1101-2021_02_08_430275 51 20 v3 v3 NN 10_1101-2021_02_08_430275 51 21 chemistry.92 chemistry.92 NNP 10_1101-2021_02_08_430275 51 22 The the DT 10_1101-2021_02_08_430275 51 23 BSA BSA NNP 10_1101-2021_02_08_430275 51 24 - - HYPH 10_1101-2021_02_08_430275 51 25 Seq Seq NNP 10_1101-2021_02_08_430275 51 26 sequencing sequencing NN 10_1101-2021_02_08_430275 51 27 data datum NNS 10_1101-2021_02_08_430275 51 28 ( ( -LRB- 10_1101-2021_02_08_430275 51 29 ERR2696318 ERR2696318 NNP 10_1101-2021_02_08_430275 51 30 : : : 10_1101-2021_02_08_430275 51 31 parent parent NN 10_1101-2021_02_08_430275 51 32 LD24;93 ld24;93 CD 10_1101-2021_02_08_430275 51 33 ERR2696319 err2696319 NN 10_1101-2021_02_08_430275 51 34 : : : 10_1101-2021_02_08_430275 51 35 parent parent NN 10_1101-2021_02_08_430275 51 36 VialoneNano VialoneNano NNP 10_1101-2021_02_08_430275 51 37 ; ; : 10_1101-2021_02_08_430275 51 38 ERR2696321 ERR2696321 NNP 10_1101-2021_02_08_430275 51 39 : : : 10_1101-2021_02_08_430275 51 40 the the DT 10_1101-2021_02_08_430275 51 41 resistant resistant JJ 10_1101-2021_02_08_430275 51 42 bulk94 bulk94 NN 10_1101-2021_02_08_430275 51 43 from from IN 10_1101-2021_02_08_430275 51 44 the the DT 10_1101-2021_02_08_430275 51 45 F2 F2 NNP 10_1101-2021_02_08_430275 51 46 population population NN 10_1101-2021_02_08_430275 51 47 ; ; : 10_1101-2021_02_08_430275 51 48 ERR2696322 ERR2696322 NNP 10_1101-2021_02_08_430275 51 49 : : : 10_1101-2021_02_08_430275 51 50 the the DT 10_1101-2021_02_08_430275 51 51 susceptible susceptible JJ 10_1101-2021_02_08_430275 51 52 bulk bulk NN 10_1101-2021_02_08_430275 51 53 from95 from95 VBD 10_1101-2021_02_08_430275 51 54 the the DT 10_1101-2021_02_08_430275 51 55 F2 F2 NNP 10_1101-2021_02_08_430275 51 56 population population NN 10_1101-2021_02_08_430275 51 57 ) ) -RRB- 10_1101-2021_02_08_430275 51 58 were be VBD 10_1101-2021_02_08_430275 51 59 downloaded download VBN 10_1101-2021_02_08_430275 51 60 from from IN 10_1101-2021_02_08_430275 51 61 the the DT 10_1101-2021_02_08_430275 51 62 European European NNP 10_1101-2021_02_08_430275 51 63 Nucleotide96 Nucleotide96 NNP 10_1101-2021_02_08_430275 51 64 Archive Archive NNP 10_1101-2021_02_08_430275 51 65 ( ( -LRB- 10_1101-2021_02_08_430275 51 66 ENA ENA NNP 10_1101-2021_02_08_430275 51 67 ) ) -RRB- 10_1101-2021_02_08_430275 51 68 using use VBG 10_1101-2021_02_08_430275 51 69 the the DT 10_1101-2021_02_08_430275 51 70 Linux Linux NNP 10_1101-2021_02_08_430275 51 71 program program NN 10_1101-2021_02_08_430275 51 72 wget wget NN 10_1101-2021_02_08_430275 51 73 , , , 10_1101-2021_02_08_430275 51 74 and and CC 10_1101-2021_02_08_430275 51 75 the the DT 10_1101-2021_02_08_430275 51 76 rice rice NN 10_1101-2021_02_08_430275 51 77 reference97 reference97 NN 10_1101-2021_02_08_430275 51 78 sequence sequence NN 10_1101-2021_02_08_430275 51 79 ( ( -LRB- 10_1101-2021_02_08_430275 51 80 Release Release NNP 10_1101-2021_02_08_430275 51 81 47 47 CD 10_1101-2021_02_08_430275 51 82 ) ) -RRB- 10_1101-2021_02_08_430275 51 83 was be VBD 10_1101-2021_02_08_430275 51 84 downloaded download VBN 10_1101-2021_02_08_430275 51 85 from from IN 10_1101-2021_02_08_430275 51 86 https://plants.ensembl.98 https://plants.ensembl.98 CD 10_1101-2021_02_08_430275 51 87 org org NN 10_1101-2021_02_08_430275 51 88 / / SYM 10_1101-2021_02_08_430275 51 89 Oryza_sativa Oryza_sativa NNP 10_1101-2021_02_08_430275 51 90 / / SYM 10_1101-2021_02_08_430275 51 91 Info Info NNP 10_1101-2021_02_08_430275 51 92 / / SYM 10_1101-2021_02_08_430275 51 93 Index Index NNP 10_1101-2021_02_08_430275 51 94 . . . 10_1101-2021_02_08_430275 52 1 Sequencing sequence VBG 10_1101-2021_02_08_430275 52 2 data datum NNS 10_1101-2021_02_08_430275 52 3 preprocessing preprocesse VBG 10_1101-2021_02_08_430275 52 4 and and CC 10_1101-2021_02_08_430275 52 5 SNP99 snp99 JJ 10_1101-2021_02_08_430275 52 6 calling calling NN 10_1101-2021_02_08_430275 52 7 were be VBD 10_1101-2021_02_08_430275 52 8 performed perform VBN 10_1101-2021_02_08_430275 52 9 as as IN 10_1101-2021_02_08_430275 52 10 described describe VBN 10_1101-2021_02_08_430275 52 11 previously previously RB 10_1101-2021_02_08_430275 52 12 ( ( -LRB- 10_1101-2021_02_08_430275 52 13 8) 8) CD 10_1101-2021_02_08_430275 52 14 . . . 10_1101-2021_02_08_430275 53 1 When when WRB 10_1101-2021_02_08_430275 53 2 analyzing100 analyzing100 NNP 10_1101-2021_02_08_430275 53 3 the the DT 10_1101-2021_02_08_430275 53 4 BSA BSA NNP 10_1101-2021_02_08_430275 53 5 - - HYPH 10_1101-2021_02_08_430275 53 6 Seq seq NN 10_1101-2021_02_08_430275 53 7 data datum NNS 10_1101-2021_02_08_430275 53 8 with with IN 10_1101-2021_02_08_430275 53 9 the the DT 10_1101-2021_02_08_430275 53 10 genome genome JJ 10_1101-2021_02_08_430275 53 11 sequences sequence NNS 10_1101-2021_02_08_430275 53 12 of of IN 10_1101-2021_02_08_430275 53 13 both both CC 10_1101-2021_02_08_430275 53 14 the the DT 10_1101-2021_02_08_430275 53 15 parents101 parents101 NNP 10_1101-2021_02_08_430275 53 16 and and CC 10_1101-2021_02_08_430275 53 17 the the DT 10_1101-2021_02_08_430275 53 18 bulks bulk NNS 10_1101-2021_02_08_430275 53 19 , , , 10_1101-2021_02_08_430275 53 20 bulk bulk NN 10_1101-2021_02_08_430275 53 21 / / SYM 10_1101-2021_02_08_430275 53 22 parent parent NN 10_1101-2021_02_08_430275 53 23 SNP SNP NNP 10_1101-2021_02_08_430275 53 24 calling calling NN 10_1101-2021_02_08_430275 53 25 was be VBD 10_1101-2021_02_08_430275 53 26 performed perform VBN 10_1101-2021_02_08_430275 53 27 separately.102 separately.102 NNP 10_1101-2021_02_08_430275 53 28 The the DT 10_1101-2021_02_08_430275 53 29 common common JJ 10_1101-2021_02_08_430275 53 30 SNPs snp NNS 10_1101-2021_02_08_430275 53 31 of of IN 10_1101-2021_02_08_430275 53 32 the the DT 10_1101-2021_02_08_430275 53 33 two two CD 10_1101-2021_02_08_430275 53 34 SNP SNP NNP 10_1101-2021_02_08_430275 53 35 datasets dataset NNS 10_1101-2021_02_08_430275 53 36 were be VBD 10_1101-2021_02_08_430275 53 37 used use VBN 10_1101-2021_02_08_430275 53 38 for for IN 10_1101-2021_02_08_430275 53 39 the103 the103 JJ 10_1101-2021_02_08_430275 53 40 downstream downstream JJ 10_1101-2021_02_08_430275 53 41 analysis.104 analysis.104 NNP 10_1101-2021_02_08_430275 53 42 The the DT 10_1101-2021_02_08_430275 53 43 SNP SNP NNP 10_1101-2021_02_08_430275 53 44 dataset dataset NN 10_1101-2021_02_08_430275 53 45 generated generate VBD 10_1101-2021_02_08_430275 53 46 via via IN 10_1101-2021_02_08_430275 53 47 SNP SNP NNP 10_1101-2021_02_08_430275 53 48 calling call VBG 10_1101-2021_02_08_430275 53 49 was be VBD 10_1101-2021_02_08_430275 53 50 processed process VBN 10_1101-2021_02_08_430275 53 51 with105 with105 NNP 10_1101-2021_02_08_430275 53 52 our -PRON- PRP$ 10_1101-2021_02_08_430275 53 53 Python Python NNP 10_1101-2021_02_08_430275 53 54 script script NN 10_1101-2021_02_08_430275 53 55 to to TO 10_1101-2021_02_08_430275 53 56 identify identify VB 10_1101-2021_02_08_430275 53 57 significant significant JJ 10_1101-2021_02_08_430275 53 58 SNP SNP NNP 10_1101-2021_02_08_430275 53 59 - - HYPH 10_1101-2021_02_08_430275 53 60 trait trait NN 10_1101-2021_02_08_430275 53 61 associations.106 associations.106 NNP 10_1101-2021_02_08_430275 53 62 A a DT 10_1101-2021_02_08_430275 53 63 single single JJ 10_1101-2021_02_08_430275 53 64 script script NN 10_1101-2021_02_08_430275 53 65 containing contain VBG 10_1101-2021_02_08_430275 53 66 all all PDT 10_1101-2021_02_08_430275 53 67 the the DT 10_1101-2021_02_08_430275 53 68 three three CD 10_1101-2021_02_08_430275 53 69 methods method NNS 10_1101-2021_02_08_430275 53 70 is be VBZ 10_1101-2021_02_08_430275 53 71 available available JJ 10_1101-2021_02_08_430275 53 72 on107 on107 NN 10_1101-2021_02_08_430275 53 73 the the DT 10_1101-2021_02_08_430275 53 74 website website NN 10_1101-2021_02_08_430275 53 75 https://github.com/dblhlx/PyBSASeq https://github.com/dblhlx/pybsaseq NN 10_1101-2021_02_08_430275 53 76 . . . 10_1101-2021_02_08_430275 54 1 The the DT 10_1101-2021_02_08_430275 54 2 workflow workflow NN 10_1101-2021_02_08_430275 54 3 of of IN 10_1101-2021_02_08_430275 54 4 the108 the108 NNS 10_1101-2021_02_08_430275 54 5 scripts script NNS 10_1101-2021_02_08_430275 54 6 is be VBZ 10_1101-2021_02_08_430275 54 7 as as IN 10_1101-2021_02_08_430275 54 8 follows:109 follows:109 NNP 10_1101-2021_02_08_430275 54 9 1 1 CD 10_1101-2021_02_08_430275 54 10 . . . 10_1101-2021_02_08_430275 55 1 Read read VB 10_1101-2021_02_08_430275 55 2 the the DT 10_1101-2021_02_08_430275 55 3 .tsv .tsv CD 10_1101-2021_02_08_430275 55 4 input input NN 10_1101-2021_02_08_430275 55 5 file file NN 10_1101-2021_02_08_430275 55 6 generated generate VBN 10_1101-2021_02_08_430275 55 7 via via IN 10_1101-2021_02_08_430275 55 8 SNP SNP NNP 10_1101-2021_02_08_430275 55 9 calling call VBG 10_1101-2021_02_08_430275 55 10 into into IN 10_1101-2021_02_08_430275 55 11 a a DT 10_1101-2021_02_08_430275 55 12 Pandas110 Pandas110 NNP 10_1101-2021_02_08_430275 55 13 DataFrame.111 DataFrame.111 NNP 10_1101-2021_02_08_430275 55 14 2 2 CD 10_1101-2021_02_08_430275 55 15 . . . 10_1101-2021_02_08_430275 56 1 Perform perform VB 10_1101-2021_02_08_430275 56 2 SNP SNP NNP 10_1101-2021_02_08_430275 56 3 filtering filter VBG 10_1101-2021_02_08_430275 56 4 on on IN 10_1101-2021_02_08_430275 56 5 the the DT 10_1101-2021_02_08_430275 56 6 Pandas Pandas NNP 10_1101-2021_02_08_430275 56 7 DataFrame.112 DataFrame.112 NNP 10_1101-2021_02_08_430275 56 8 3 3 CD 10_1101-2021_02_08_430275 56 9 . . . 10_1101-2021_02_08_430275 57 1 Identify identify VB 10_1101-2021_02_08_430275 57 2 the the DT 10_1101-2021_02_08_430275 57 3 significant significant JJ 10_1101-2021_02_08_430275 57 4 SNPs snp NNS 10_1101-2021_02_08_430275 57 5 ( ( -LRB- 10_1101-2021_02_08_430275 57 6 sSNPs ssnps NN 10_1101-2021_02_08_430275 57 7 ) ) -RRB- 10_1101-2021_02_08_430275 57 8 via via IN 10_1101-2021_02_08_430275 57 9 Fisher Fisher NNP 10_1101-2021_02_08_430275 57 10 ’s ’s POS 10_1101-2021_02_08_430275 57 11 exact exact JJ 10_1101-2021_02_08_430275 57 12 test113 test113 FW 10_1101-2021_02_08_430275 57 13 ( ( -LRB- 10_1101-2021_02_08_430275 57 14 the the DT 10_1101-2021_02_08_430275 57 15 significant significant JJ 10_1101-2021_02_08_430275 57 16 structural structural JJ 10_1101-2021_02_08_430275 57 17 variant variant JJ 10_1101-2021_02_08_430275 57 18 method method NN 10_1101-2021_02_08_430275 57 19 ) ) -RRB- 10_1101-2021_02_08_430275 57 20 , , , 10_1101-2021_02_08_430275 57 21 calculate calculate VB 10_1101-2021_02_08_430275 57 22 the the DT 10_1101-2021_02_08_430275 57 23 ΔAF114 ΔAF114 NNP 10_1101-2021_02_08_430275 57 24 ( ( -LRB- 10_1101-2021_02_08_430275 57 25 allele allele NNP 10_1101-2021_02_08_430275 57 26 frequency frequency NN 10_1101-2021_02_08_430275 57 27 difference difference NN 10_1101-2021_02_08_430275 57 28 between between IN 10_1101-2021_02_08_430275 57 29 bulks bulk NNS 10_1101-2021_02_08_430275 57 30 ) ) -RRB- 10_1101-2021_02_08_430275 57 31 values value NNS 10_1101-2021_02_08_430275 57 32 ( ( -LRB- 10_1101-2021_02_08_430275 57 33 the the DT 10_1101-2021_02_08_430275 57 34 allele115 allele115 NNP 10_1101-2021_02_08_430275 57 35 frequency frequency NN 10_1101-2021_02_08_430275 57 36 method method NN 10_1101-2021_02_08_430275 57 37 ) ) -RRB- 10_1101-2021_02_08_430275 57 38 , , , 10_1101-2021_02_08_430275 57 39 or or CC 10_1101-2021_02_08_430275 57 40 calculate calculate VB 10_1101-2021_02_08_430275 57 41 the the DT 10_1101-2021_02_08_430275 57 42 G g NN 10_1101-2021_02_08_430275 57 43 - - HYPH 10_1101-2021_02_08_430275 57 44 statistic statistic NN 10_1101-2021_02_08_430275 57 45 values value NNS 10_1101-2021_02_08_430275 57 46 ( ( -LRB- 10_1101-2021_02_08_430275 57 47 the116 the116 NNP 10_1101-2021_02_08_430275 57 48 G g NN 10_1101-2021_02_08_430275 57 49 - - HYPH 10_1101-2021_02_08_430275 57 50 statistic statistic NN 10_1101-2021_02_08_430275 57 51 method method NN 10_1101-2021_02_08_430275 57 52 ) ) -RRB- 10_1101-2021_02_08_430275 57 53 using use VBG 10_1101-2021_02_08_430275 57 54 the the DT 10_1101-2021_02_08_430275 57 55 four four CD 10_1101-2021_02_08_430275 57 56 AD ad NN 10_1101-2021_02_08_430275 57 57 values value NNS 10_1101-2021_02_08_430275 57 58 ( ( -LRB- 10_1101-2021_02_08_430275 57 59 ADref1 ADref1 NNP 10_1101-2021_02_08_430275 57 60 and117 and117 NNP 10_1101-2021_02_08_430275 57 61 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 57 62 of of IN 10_1101-2021_02_08_430275 57 63 bulk bulk NNP 10_1101-2021_02_08_430275 57 64 1 1 CD 10_1101-2021_02_08_430275 57 65 and and CC 10_1101-2021_02_08_430275 57 66 ADref2 ADref2 NNP 10_1101-2021_02_08_430275 57 67 and and CC 10_1101-2021_02_08_430275 57 68 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 57 69 of of IN 10_1101-2021_02_08_430275 57 70 bulk bulk NNP 10_1101-2021_02_08_430275 57 71 2 2 CD 10_1101-2021_02_08_430275 57 72 ) ) -RRB- 10_1101-2021_02_08_430275 57 73 of of IN 10_1101-2021_02_08_430275 57 74 each118 each118 NNP 10_1101-2021_02_08_430275 57 75 SNP SNP NNP 10_1101-2021_02_08_430275 57 76 in in IN 10_1101-2021_02_08_430275 57 77 the the DT 10_1101-2021_02_08_430275 57 78 filtered filter VBN 10_1101-2021_02_08_430275 57 79 Pandas Pandas NNP 10_1101-2021_02_08_430275 57 80 DataFrame.119 DataFrame.119 NNP 10_1101-2021_02_08_430275 57 81 4 4 CD 10_1101-2021_02_08_430275 57 82 . . . 10_1101-2021_02_08_430275 58 1 Use use VB 10_1101-2021_02_08_430275 58 2 the the DT 10_1101-2021_02_08_430275 58 3 sliding slide VBG 10_1101-2021_02_08_430275 58 4 window window NN 10_1101-2021_02_08_430275 58 5 algorithm algorithm NN 10_1101-2021_02_08_430275 58 6 to to TO 10_1101-2021_02_08_430275 58 7 plot plot VB 10_1101-2021_02_08_430275 58 8 the the DT 10_1101-2021_02_08_430275 58 9 sSNP ssnp NN 10_1101-2021_02_08_430275 58 10 / / SYM 10_1101-2021_02_08_430275 58 11 totalSNP120 totalSNP120 NNP 10_1101-2021_02_08_430275 58 12 ratios ratio NNS 10_1101-2021_02_08_430275 58 13 , , , 10_1101-2021_02_08_430275 58 14 the the DT 10_1101-2021_02_08_430275 58 15 ΔAF δaf NN 10_1101-2021_02_08_430275 58 16 values value NNS 10_1101-2021_02_08_430275 58 17 , , , 10_1101-2021_02_08_430275 58 18 or or CC 10_1101-2021_02_08_430275 58 19 the the DT 10_1101-2021_02_08_430275 58 20 G g NN 10_1101-2021_02_08_430275 58 21 - - HYPH 10_1101-2021_02_08_430275 58 22 statistic statistic NN 10_1101-2021_02_08_430275 58 23 values value NNS 10_1101-2021_02_08_430275 58 24 against against IN 10_1101-2021_02_08_430275 58 25 their121 their121 NNP 10_1101-2021_02_08_430275 58 26 genomic genomic JJ 10_1101-2021_02_08_430275 58 27 positions.122 positions.122 NNP 10_1101-2021_02_08_430275 58 28 5 5 CD 10_1101-2021_02_08_430275 58 29 . . . 10_1101-2021_02_08_430275 59 1 Estimate estimate VB 10_1101-2021_02_08_430275 59 2 the the DT 10_1101-2021_02_08_430275 59 3 threshold threshold NN 10_1101-2021_02_08_430275 59 4 of of IN 10_1101-2021_02_08_430275 59 5 the the DT 10_1101-2021_02_08_430275 59 6 sSNP ssnp NN 10_1101-2021_02_08_430275 59 7 / / SYM 10_1101-2021_02_08_430275 59 8 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 59 9 ratio ratio NN 10_1101-2021_02_08_430275 59 10 , , , 10_1101-2021_02_08_430275 59 11 the the DT 10_1101-2021_02_08_430275 59 12 ΔAF,123 ΔAF,123 NNP 10_1101-2021_02_08_430275 59 13 or or CC 10_1101-2021_02_08_430275 59 14 the the DT 10_1101-2021_02_08_430275 59 15 G g NN 10_1101-2021_02_08_430275 59 16 - - HYPH 10_1101-2021_02_08_430275 59 17 statistic statistic NN 10_1101-2021_02_08_430275 59 18 via via IN 10_1101-2021_02_08_430275 59 19 simulation simulation NN 10_1101-2021_02_08_430275 59 20 . . . 10_1101-2021_02_08_430275 60 1 The the DT 10_1101-2021_02_08_430275 60 2 thresholds threshold NNS 10_1101-2021_02_08_430275 60 3 are be VBP 10_1101-2021_02_08_430275 60 4 used use VBN 10_1101-2021_02_08_430275 60 5 to124 to124 NN 10_1101-2021_02_08_430275 60 6 identify identify VB 10_1101-2021_02_08_430275 60 7 the the DT 10_1101-2021_02_08_430275 60 8 significant significant JJ 10_1101-2021_02_08_430275 60 9 peaks peak NNS 10_1101-2021_02_08_430275 60 10 / / SYM 10_1101-2021_02_08_430275 60 11 valleys valley NNS 10_1101-2021_02_08_430275 60 12 in in IN 10_1101-2021_02_08_430275 60 13 the the DT 10_1101-2021_02_08_430275 60 14 plots plot NNS 10_1101-2021_02_08_430275 60 15 generated generate VBD 10_1101-2021_02_08_430275 60 16 in125 in125 NNP 10_1101-2021_02_08_430275 60 17 step step NN 10_1101-2021_02_08_430275 60 18 4.126 4.126 CD 10_1101-2021_02_08_430275 60 19 Identification Identification NNP 10_1101-2021_02_08_430275 60 20 of of IN 10_1101-2021_02_08_430275 60 21 the the DT 10_1101-2021_02_08_430275 60 22 sSNPs ssnps CD 10_1101-2021_02_08_430275 60 23 , , , 10_1101-2021_02_08_430275 60 24 calculation calculation NN 10_1101-2021_02_08_430275 60 25 of of IN 10_1101-2021_02_08_430275 60 26 the the DT 10_1101-2021_02_08_430275 60 27 sSNP ssnp NN 10_1101-2021_02_08_430275 60 28 / / SYM 10_1101-2021_02_08_430275 60 29 totalSNP127 totalSNP127 NNP 10_1101-2021_02_08_430275 60 30 ratios ratio NNS 10_1101-2021_02_08_430275 60 31 , , , 10_1101-2021_02_08_430275 60 32 the the DT 10_1101-2021_02_08_430275 60 33 G g NN 10_1101-2021_02_08_430275 60 34 - - HYPH 10_1101-2021_02_08_430275 60 35 statistic statistic NN 10_1101-2021_02_08_430275 60 36 values value NNS 10_1101-2021_02_08_430275 60 37 , , , 10_1101-2021_02_08_430275 60 38 or or CC 10_1101-2021_02_08_430275 60 39 the the DT 10_1101-2021_02_08_430275 60 40 ΔAF δaf NN 10_1101-2021_02_08_430275 60 41 values value NNS 10_1101-2021_02_08_430275 60 42 , , , 10_1101-2021_02_08_430275 60 43 and and CC 10_1101-2021_02_08_430275 60 44 estimation128 estimation128 NN 10_1101-2021_02_08_430275 60 45 of of IN 10_1101-2021_02_08_430275 60 46 their -PRON- PRP$ 10_1101-2021_02_08_430275 60 47 thresholds threshold NNS 10_1101-2021_02_08_430275 60 48 were be VBD 10_1101-2021_02_08_430275 60 49 carried carry VBN 10_1101-2021_02_08_430275 60 50 out out RP 10_1101-2021_02_08_430275 60 51 as as IN 10_1101-2021_02_08_430275 60 52 described describe VBN 10_1101-2021_02_08_430275 60 53 previously previously RB 10_1101-2021_02_08_430275 60 54 ( ( -LRB- 10_1101-2021_02_08_430275 60 55 8).129 8).129 CD 10_1101-2021_02_08_430275 60 56 The the DT 10_1101-2021_02_08_430275 60 57 99.5th 99.5th NNP 10_1101-2021_02_08_430275 60 58 percentile percentile NN 10_1101-2021_02_08_430275 60 59 of of IN 10_1101-2021_02_08_430275 60 60 10 10 CD 10_1101-2021_02_08_430275 60 61 000 000 CD 10_1101-2021_02_08_430275 60 62 simulated simulate VBN 10_1101-2021_02_08_430275 60 63 sSNP ssnp NN 10_1101-2021_02_08_430275 60 64 / / SYM 10_1101-2021_02_08_430275 60 65 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 60 66 ratios130 ratios130 IN 10_1101-2021_02_08_430275 60 67 or or CC 10_1101-2021_02_08_430275 60 68 G g NN 10_1101-2021_02_08_430275 60 69 - - HYPH 10_1101-2021_02_08_430275 60 70 statistic statistic NN 10_1101-2021_02_08_430275 60 71 values value NNS 10_1101-2021_02_08_430275 60 72 was be VBD 10_1101-2021_02_08_430275 60 73 used use VBN 10_1101-2021_02_08_430275 60 74 as as IN 10_1101-2021_02_08_430275 60 75 the the DT 10_1101-2021_02_08_430275 60 76 threshold threshold NN 10_1101-2021_02_08_430275 60 77 for for IN 10_1101-2021_02_08_430275 60 78 the the DT 10_1101-2021_02_08_430275 60 79 significant131 significant131 NNP 10_1101-2021_02_08_430275 60 80 structural structural JJ 10_1101-2021_02_08_430275 60 81 variant variant JJ 10_1101-2021_02_08_430275 60 82 method method NN 10_1101-2021_02_08_430275 60 83 or or CC 10_1101-2021_02_08_430275 60 84 the the DT 10_1101-2021_02_08_430275 60 85 G g NN 10_1101-2021_02_08_430275 60 86 - - HYPH 10_1101-2021_02_08_430275 60 87 statistic statistic NN 10_1101-2021_02_08_430275 60 88 method method NN 10_1101-2021_02_08_430275 60 89 , , , 10_1101-2021_02_08_430275 60 90 and and CC 10_1101-2021_02_08_430275 60 91 the the DT 10_1101-2021_02_08_430275 60 92 99%132 99%132 CD 10_1101-2021_02_08_430275 60 93 confidence confidence NN 10_1101-2021_02_08_430275 60 94 interval interval NN 10_1101-2021_02_08_430275 60 95 of of IN 10_1101-2021_02_08_430275 60 96 10 10 CD 10_1101-2021_02_08_430275 60 97 000 000 CD 10_1101-2021_02_08_430275 60 98 simulated simulate VBN 10_1101-2021_02_08_430275 60 99 ΔAF δaf NN 10_1101-2021_02_08_430275 60 100 values value NNS 10_1101-2021_02_08_430275 60 101 was be VBD 10_1101-2021_02_08_430275 60 102 used use VBN 10_1101-2021_02_08_430275 60 103 as133 as133 CC 10_1101-2021_02_08_430275 60 104 the the DT 10_1101-2021_02_08_430275 60 105 threshold threshold NN 10_1101-2021_02_08_430275 60 106 for for IN 10_1101-2021_02_08_430275 60 107 the the DT 10_1101-2021_02_08_430275 60 108 allele allele NNP 10_1101-2021_02_08_430275 60 109 frequency frequency NN 10_1101-2021_02_08_430275 60 110 method method NN 10_1101-2021_02_08_430275 60 111 . . . 10_1101-2021_02_08_430275 61 1 For for IN 10_1101-2021_02_08_430275 61 2 all all DT 10_1101-2021_02_08_430275 61 3 methods,134 methods,134 NN 10_1101-2021_02_08_430275 61 4 the the DT 10_1101-2021_02_08_430275 61 5 size size NN 10_1101-2021_02_08_430275 61 6 of of IN 10_1101-2021_02_08_430275 61 7 the the DT 10_1101-2021_02_08_430275 61 8 sliding slide VBG 10_1101-2021_02_08_430275 61 9 windows window NNS 10_1101-2021_02_08_430275 61 10 is be VBZ 10_1101-2021_02_08_430275 61 11 2 2 CD 10_1101-2021_02_08_430275 61 12 Mb mb NN 10_1101-2021_02_08_430275 61 13 and and CC 10_1101-2021_02_08_430275 61 14 the the DT 10_1101-2021_02_08_430275 61 15 incremental incremental JJ 10_1101-2021_02_08_430275 61 16 step step NN 10_1101-2021_02_08_430275 61 17 is135 is135 NNP 10_1101-2021_02_08_430275 61 18 10 10 CD 10_1101-2021_02_08_430275 61 19 kb kb NN 10_1101-2021_02_08_430275 61 20 . . . 10_1101-2021_02_08_430275 62 1 In in IN 10_1101-2021_02_08_430275 62 2 our -PRON- PRP$ 10_1101-2021_02_08_430275 62 3 previous previous JJ 10_1101-2021_02_08_430275 62 4 work work NN 10_1101-2021_02_08_430275 62 5 , , , 10_1101-2021_02_08_430275 62 6 a a DT 10_1101-2021_02_08_430275 62 7 parent parent NN 10_1101-2021_02_08_430275 62 8 was be VBD 10_1101-2021_02_08_430275 62 9 the the DT 10_1101-2021_02_08_430275 62 10 japonica japonica NNP 10_1101-2021_02_08_430275 62 11 rice rice NNP 10_1101-2021_02_08_430275 62 12 cultivar136 cultivar136 NNP 10_1101-2021_02_08_430275 62 13 nipponbare nipponbare NN 10_1101-2021_02_08_430275 62 14 , , , 10_1101-2021_02_08_430275 62 15 and and CC 10_1101-2021_02_08_430275 62 16 its -PRON- PRP$ 10_1101-2021_02_08_430275 62 17 genome genome JJ 10_1101-2021_02_08_430275 62 18 sequences sequence NNS 10_1101-2021_02_08_430275 62 19 were be VBD 10_1101-2021_02_08_430275 62 20 used use VBN 10_1101-2021_02_08_430275 62 21 as as IN 10_1101-2021_02_08_430275 62 22 the the DT 10_1101-2021_02_08_430275 62 23 reference reference NN 10_1101-2021_02_08_430275 62 24 137 137 CD 10_1101-2021_02_08_430275 62 25 sequences sequence NNS 10_1101-2021_02_08_430275 62 26 for for IN 10_1101-2021_02_08_430275 62 27 SNP SNP NNP 10_1101-2021_02_08_430275 62 28 / / SYM 10_1101-2021_02_08_430275 62 29 InDel InDel NNP 10_1101-2021_02_08_430275 62 30 calling call VBG 10_1101-2021_02_08_430275 62 31 . . . 10_1101-2021_02_08_430275 63 1 In in IN 10_1101-2021_02_08_430275 63 2 the the DT 10_1101-2021_02_08_430275 63 3 current current JJ 10_1101-2021_02_08_430275 63 4 dataset dataset NN 10_1101-2021_02_08_430275 63 5 , , , 10_1101-2021_02_08_430275 63 6 the the DT 10_1101-2021_02_08_430275 63 7 parents parent NNS 10_1101-2021_02_08_430275 63 8 138 138 CD 10_1101-2021_02_08_430275 63 9 were be VBD 10_1101-2021_02_08_430275 63 10 LD24 LD24 NNP 10_1101-2021_02_08_430275 63 11 and and CC 10_1101-2021_02_08_430275 63 12 VialoneNano VialoneNano NNP 10_1101-2021_02_08_430275 63 13 ; ; : 10_1101-2021_02_08_430275 63 14 many many JJ 10_1101-2021_02_08_430275 63 15 false false JJ 10_1101-2021_02_08_430275 63 16 - - HYPH 10_1101-2021_02_08_430275 63 17 positive positive JJ 10_1101-2021_02_08_430275 63 18 SNPs snp NNS 10_1101-2021_02_08_430275 63 19 / / SYM 10_1101-2021_02_08_430275 63 20 InDels indel NNS 10_1101-2021_02_08_430275 63 21 and and CC 10_1101-2021_02_08_430275 63 22 139 139 CD 10_1101-2021_02_08_430275 63 23 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 63 24 loci loci NN 10_1101-2021_02_08_430275 63 25 in in IN 10_1101-2021_02_08_430275 63 26 the the DT 10_1101-2021_02_08_430275 63 27 parents parent NNS 10_1101-2021_02_08_430275 63 28 would would MD 10_1101-2021_02_08_430275 63 29 be be VB 10_1101-2021_02_08_430275 63 30 included include VBN 10_1101-2021_02_08_430275 63 31 in in IN 10_1101-2021_02_08_430275 63 32 the the DT 10_1101-2021_02_08_430275 63 33 dataset dataset NN 10_1101-2021_02_08_430275 63 34 if if IN 10_1101-2021_02_08_430275 63 35 140 140 CD 10_1101-2021_02_08_430275 63 36 analyzing analyze VBG 10_1101-2021_02_08_430275 63 37 the the DT 10_1101-2021_02_08_430275 63 38 BSA BSA NNP 10_1101-2021_02_08_430275 63 39 - - HYPH 10_1101-2021_02_08_430275 63 40 Seq Seq NNP 10_1101-2021_02_08_430275 63 41 data datum NNS 10_1101-2021_02_08_430275 63 42 using use VBG 10_1101-2021_02_08_430275 63 43 the the DT 10_1101-2021_02_08_430275 63 44 original original JJ 10_1101-2021_02_08_430275 63 45 script script NN 10_1101-2021_02_08_430275 63 46 . . . 10_1101-2021_02_08_430275 64 1 Hence hence RB 10_1101-2021_02_08_430275 64 2 , , , 10_1101-2021_02_08_430275 64 3 SNP SNP NNP 10_1101-2021_02_08_430275 64 4 141 141 CD 10_1101-2021_02_08_430275 64 5 filtering filtering NN 10_1101-2021_02_08_430275 64 6 is be VBZ 10_1101-2021_02_08_430275 64 7 carried carry VBN 10_1101-2021_02_08_430275 64 8 out out RP 10_1101-2021_02_08_430275 64 9 a a DT 10_1101-2021_02_08_430275 64 10 little little JJ 10_1101-2021_02_08_430275 64 11 differently differently RB 10_1101-2021_02_08_430275 64 12 from from IN 10_1101-2021_02_08_430275 64 13 previously previously RB 10_1101-2021_02_08_430275 64 14 described describe VBN 10_1101-2021_02_08_430275 64 15 142 142 CD 10_1101-2021_02_08_430275 64 16 ( ( -LRB- 10_1101-2021_02_08_430275 64 17 8) 8) CD 10_1101-2021_02_08_430275 64 18 , , , 10_1101-2021_02_08_430275 64 19 and and CC 10_1101-2021_02_08_430275 64 20 its -PRON- PRP$ 10_1101-2021_02_08_430275 64 21 details detail NNS 10_1101-2021_02_08_430275 64 22 are be VBP 10_1101-2021_02_08_430275 64 23 below below RB 10_1101-2021_02_08_430275 64 24 ( ( -LRB- 10_1101-2021_02_08_430275 64 25 see see VB 10_1101-2021_02_08_430275 64 26 Table table NN 10_1101-2021_02_08_430275 64 27 S1 S1 NNS 10_1101-2021_02_08_430275 64 28 for for IN 10_1101-2021_02_08_430275 64 29 examples example NNS 10_1101-2021_02_08_430275 64 30 ) ) -RRB- 10_1101-2021_02_08_430275 64 31 : : : 10_1101-2021_02_08_430275 64 32 143 143 CD 10_1101-2021_02_08_430275 64 33 • • NN 10_1101-2021_02_08_430275 64 34 Unmapped unmapped JJ 10_1101-2021_02_08_430275 64 35 SNPs snp NNS 10_1101-2021_02_08_430275 64 36 or or CC 10_1101-2021_02_08_430275 64 37 SNPs snp NNS 10_1101-2021_02_08_430275 64 38 mapped map VBN 10_1101-2021_02_08_430275 64 39 to to IN 10_1101-2021_02_08_430275 64 40 the the DT 10_1101-2021_02_08_430275 64 41 mitochondrial mitochondrial NN 10_1101-2021_02_08_430275 64 42 or or CC 10_1101-2021_02_08_430275 64 43 144 144 CD 10_1101-2021_02_08_430275 64 44 chloroplast chloroplast NN 10_1101-2021_02_08_430275 64 45 genome genome NN 10_1101-2021_02_08_430275 64 46 145 145 CD 10_1101-2021_02_08_430275 64 47 • • NN 10_1101-2021_02_08_430275 64 48 SNPs snp NNS 10_1101-2021_02_08_430275 64 49 with with IN 10_1101-2021_02_08_430275 64 50 an an DT 10_1101-2021_02_08_430275 64 51 ‘ ' `` 10_1101-2021_02_08_430275 64 52 NA NA NNP 10_1101-2021_02_08_430275 64 53 ’ ' '' 10_1101-2021_02_08_430275 64 54 value value NN 10_1101-2021_02_08_430275 64 55 in in IN 10_1101-2021_02_08_430275 64 56 any any DT 10_1101-2021_02_08_430275 64 57 column column NN 10_1101-2021_02_08_430275 64 58 of of IN 10_1101-2021_02_08_430275 64 59 the the DT 10_1101-2021_02_08_430275 64 60 DataFrame DataFrame NNP 10_1101-2021_02_08_430275 64 61 146 146 CD 10_1101-2021_02_08_430275 64 62 • • NNP 10_1101-2021_02_08_430275 64 63 SNPs SNPs NNPS 10_1101-2021_02_08_430275 64 64 with with IN 10_1101-2021_02_08_430275 64 65 zero zero CD 10_1101-2021_02_08_430275 64 66 REF REF NNP 10_1101-2021_02_08_430275 64 67 read read NN 10_1101-2021_02_08_430275 64 68 and and CC 10_1101-2021_02_08_430275 64 69 a a DT 10_1101-2021_02_08_430275 64 70 single single JJ 10_1101-2021_02_08_430275 64 71 ALT ALT NNP 10_1101-2021_02_08_430275 64 72 allele allele NN 10_1101-2021_02_08_430275 64 73 in in IN 10_1101-2021_02_08_430275 64 74 both both DT 10_1101-2021_02_08_430275 64 75 147 147 CD 10_1101-2021_02_08_430275 64 76 bulks bulk NNS 10_1101-2021_02_08_430275 64 77 / / SYM 10_1101-2021_02_08_430275 64 78 parents parent NNS 10_1101-2021_02_08_430275 64 79 148 148 CD 10_1101-2021_02_08_430275 64 80 • • NN 10_1101-2021_02_08_430275 64 81 SNPs snp NNS 10_1101-2021_02_08_430275 64 82 with with IN 10_1101-2021_02_08_430275 64 83 three three CD 10_1101-2021_02_08_430275 64 84 or or CC 10_1101-2021_02_08_430275 64 85 more more JJR 10_1101-2021_02_08_430275 64 86 ALT ALT NNP 10_1101-2021_02_08_430275 64 87 alleles allele NNS 10_1101-2021_02_08_430275 64 88 in in IN 10_1101-2021_02_08_430275 64 89 any any DT 10_1101-2021_02_08_430275 64 90 bulk bulk NN 10_1101-2021_02_08_430275 64 91 / / SYM 10_1101-2021_02_08_430275 64 92 parent parent NN 10_1101-2021_02_08_430275 64 93 149 149 CD 10_1101-2021_02_08_430275 64 94 • • NNP 10_1101-2021_02_08_430275 64 95 SNPs SNPs NNPS 10_1101-2021_02_08_430275 64 96 with with IN 10_1101-2021_02_08_430275 64 97 two two CD 10_1101-2021_02_08_430275 64 98 ALT ALT NNP 10_1101-2021_02_08_430275 64 99 alleles allele NNS 10_1101-2021_02_08_430275 64 100 and and CC 10_1101-2021_02_08_430275 64 101 its -PRON- PRP$ 10_1101-2021_02_08_430275 64 102 REF REF NNP 10_1101-2021_02_08_430275 64 103 read read NN 10_1101-2021_02_08_430275 64 104 is be VBZ 10_1101-2021_02_08_430275 64 105 not not RB 10_1101-2021_02_08_430275 64 106 zero zero CD 10_1101-2021_02_08_430275 64 107 in in IN 10_1101-2021_02_08_430275 64 108 150 150 CD 10_1101-2021_02_08_430275 64 109 any any DT 10_1101-2021_02_08_430275 64 110 bulk bulk NN 10_1101-2021_02_08_430275 64 111 / / SYM 10_1101-2021_02_08_430275 64 112 parent parent NN 10_1101-2021_02_08_430275 64 113 151 151 CD 10_1101-2021_02_08_430275 64 114 • • NNP 10_1101-2021_02_08_430275 64 115 SNPs SNPs NNPS 10_1101-2021_02_08_430275 64 116 in in IN 10_1101-2021_02_08_430275 64 117 which which WDT 10_1101-2021_02_08_430275 64 118 the the DT 10_1101-2021_02_08_430275 64 119 bulk bulk NN 10_1101-2021_02_08_430275 64 120 / / SYM 10_1101-2021_02_08_430275 64 121 parent parent NN 10_1101-2021_02_08_430275 64 122 genotypes genotype NNS 10_1101-2021_02_08_430275 64 123 do do VBP 10_1101-2021_02_08_430275 64 124 not not RB 10_1101-2021_02_08_430275 64 125 agree agree VB 10_1101-2021_02_08_430275 64 126 with with IN 10_1101-2021_02_08_430275 64 127 152 152 CD 10_1101-2021_02_08_430275 64 128 the the DT 10_1101-2021_02_08_430275 64 129 REF REF NNP 10_1101-2021_02_08_430275 64 130 / / SYM 10_1101-2021_02_08_430275 64 131 ALT ALT NNP 10_1101-2021_02_08_430275 64 132 bases basis NNS 10_1101-2021_02_08_430275 64 133 153 153 CD 10_1101-2021_02_08_430275 64 134 • • NN 10_1101-2021_02_08_430275 64 135 SNPs snp NNS 10_1101-2021_02_08_430275 64 136 in in IN 10_1101-2021_02_08_430275 64 137 which which WDT 10_1101-2021_02_08_430275 64 138 the the DT 10_1101-2021_02_08_430275 64 139 bulk bulk NN 10_1101-2021_02_08_430275 64 140 / / SYM 10_1101-2021_02_08_430275 64 141 parent parent NN 10_1101-2021_02_08_430275 64 142 genotypes genotype NNS 10_1101-2021_02_08_430275 64 143 are be VBP 10_1101-2021_02_08_430275 64 144 not not RB 10_1101-2021_02_08_430275 64 145 consistent consistent JJ 10_1101-2021_02_08_430275 64 146 154 154 CD 10_1101-2021_02_08_430275 64 147 with with IN 10_1101-2021_02_08_430275 64 148 the the DT 10_1101-2021_02_08_430275 64 149 AD ad NN 10_1101-2021_02_08_430275 64 150 values value NNS 10_1101-2021_02_08_430275 64 151 155 155 CD 10_1101-2021_02_08_430275 64 152 • • VBP 10_1101-2021_02_08_430275 64 153 SNPs snp NNS 10_1101-2021_02_08_430275 64 154 with with IN 10_1101-2021_02_08_430275 64 155 a a DT 10_1101-2021_02_08_430275 64 156 genotype genotype NN 10_1101-2021_02_08_430275 64 157 quality quality NN 10_1101-2021_02_08_430275 64 158 ( ( -LRB- 10_1101-2021_02_08_430275 64 159 GQ GQ NNP 10_1101-2021_02_08_430275 64 160 ) ) -RRB- 10_1101-2021_02_08_430275 64 161 score score VBP 10_1101-2021_02_08_430275 64 162 less less JJR 10_1101-2021_02_08_430275 64 163 than than IN 10_1101-2021_02_08_430275 64 164 20 20 CD 10_1101-2021_02_08_430275 64 165 in in IN 10_1101-2021_02_08_430275 64 166 any any DT 10_1101-2021_02_08_430275 64 167 156 156 CD 10_1101-2021_02_08_430275 64 168 bulk bulk NN 10_1101-2021_02_08_430275 64 169 157 157 CD 10_1101-2021_02_08_430275 64 170 • • NN 10_1101-2021_02_08_430275 64 171 SNPs snp NNS 10_1101-2021_02_08_430275 64 172 with with IN 10_1101-2021_02_08_430275 64 173 very very RB 10_1101-2021_02_08_430275 64 174 high high JJ 10_1101-2021_02_08_430275 64 175 reads read NNS 10_1101-2021_02_08_430275 64 176 158 158 CD 10_1101-2021_02_08_430275 64 177 • • NN 10_1101-2021_02_08_430275 64 178 SNPs snp NNS 10_1101-2021_02_08_430275 64 179 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 64 180 in in IN 10_1101-2021_02_08_430275 64 181 any any DT 10_1101-2021_02_08_430275 64 182 parent parent NN 10_1101-2021_02_08_430275 64 183 when when WRB 10_1101-2021_02_08_430275 64 184 parental parental JJ 10_1101-2021_02_08_430275 64 185 genome genome NN 10_1101-2021_02_08_430275 64 186 se- se- VBP 10_1101-2021_02_08_430275 64 187 159 159 CD 10_1101-2021_02_08_430275 64 188 quences quence NNS 10_1101-2021_02_08_430275 64 189 are be VBP 10_1101-2021_02_08_430275 64 190 available available JJ 10_1101-2021_02_08_430275 64 191 160 160 CD 10_1101-2021_02_08_430275 64 192 Additionally additionally RB 10_1101-2021_02_08_430275 64 193 , , , 10_1101-2021_02_08_430275 64 194 for for IN 10_1101-2021_02_08_430275 64 195 SNPs snp NNS 10_1101-2021_02_08_430275 64 196 with with IN 10_1101-2021_02_08_430275 64 197 two two CD 10_1101-2021_02_08_430275 64 198 ALT ALT NNP 10_1101-2021_02_08_430275 64 199 alleles allele NNS 10_1101-2021_02_08_430275 64 200 and and CC 10_1101-2021_02_08_430275 64 201 zero zero CD 10_1101-2021_02_08_430275 64 202 REF REF NNP 10_1101-2021_02_08_430275 64 203 read read VBD 10_1101-2021_02_08_430275 64 204 161 161 CD 10_1101-2021_02_08_430275 64 205 in in IN 10_1101-2021_02_08_430275 64 206 both both DT 10_1101-2021_02_08_430275 64 207 bulks bulk NNS 10_1101-2021_02_08_430275 64 208 / / SYM 10_1101-2021_02_08_430275 64 209 parents parent NNS 10_1101-2021_02_08_430275 64 210 , , , 10_1101-2021_02_08_430275 64 211 the the DT 10_1101-2021_02_08_430275 64 212 REF REF NNP 10_1101-2021_02_08_430275 64 213 allele allele NNP 10_1101-2021_02_08_430275 64 214 is be VBZ 10_1101-2021_02_08_430275 64 215 replaced replace VBN 10_1101-2021_02_08_430275 64 216 with with IN 10_1101-2021_02_08_430275 64 217 the the DT 10_1101-2021_02_08_430275 64 218 first first JJ 10_1101-2021_02_08_430275 64 219 allele allele NNP 10_1101-2021_02_08_430275 64 220 162 162 CD 10_1101-2021_02_08_430275 64 221 in in IN 10_1101-2021_02_08_430275 64 222 the the DT 10_1101-2021_02_08_430275 64 223 ‘ ' `` 10_1101-2021_02_08_430275 64 224 ALT ALT NNP 10_1101-2021_02_08_430275 64 225 ’ ' '' 10_1101-2021_02_08_430275 64 226 field field NN 10_1101-2021_02_08_430275 64 227 , , , 10_1101-2021_02_08_430275 64 228 its -PRON- PRP$ 10_1101-2021_02_08_430275 64 229 ALT ALT NNP 10_1101-2021_02_08_430275 64 230 allele allele NNP 10_1101-2021_02_08_430275 64 231 is be VBZ 10_1101-2021_02_08_430275 64 232 replaced replace VBN 10_1101-2021_02_08_430275 64 233 with with IN 10_1101-2021_02_08_430275 64 234 the the DT 10_1101-2021_02_08_430275 64 235 second second JJ 10_1101-2021_02_08_430275 64 236 allele allele NNP 10_1101-2021_02_08_430275 64 237 163 163 CD 10_1101-2021_02_08_430275 64 238 in in IN 10_1101-2021_02_08_430275 64 239 the the DT 10_1101-2021_02_08_430275 64 240 original original JJ 10_1101-2021_02_08_430275 64 241 ‘ ' `` 10_1101-2021_02_08_430275 64 242 ALT ALT NNP 10_1101-2021_02_08_430275 64 243 ’ ' '' 10_1101-2021_02_08_430275 64 244 field field NN 10_1101-2021_02_08_430275 64 245 . . . 10_1101-2021_02_08_430275 65 1 The the DT 10_1101-2021_02_08_430275 65 2 REF REF NNP 10_1101-2021_02_08_430275 65 3 read read VBD 10_1101-2021_02_08_430275 65 4 , , , 10_1101-2021_02_08_430275 65 5 and and CC 10_1101-2021_02_08_430275 65 6 a a DT 10_1101-2021_02_08_430275 65 7 comma comma NN 10_1101-2021_02_08_430275 65 8 after after IN 10_1101-2021_02_08_430275 65 9 it -PRON- PRP 10_1101-2021_02_08_430275 65 10 , , , 10_1101-2021_02_08_430275 65 11 164 164 CD 10_1101-2021_02_08_430275 65 12 are be VBP 10_1101-2021_02_08_430275 65 13 removed remove VBN 10_1101-2021_02_08_430275 65 14 from from IN 10_1101-2021_02_08_430275 65 15 both both CC 10_1101-2021_02_08_430275 65 16 the the DT 10_1101-2021_02_08_430275 65 17 allele allele NNP 10_1101-2021_02_08_430275 65 18 depth depth NN 10_1101-2021_02_08_430275 65 19 ( ( -LRB- 10_1101-2021_02_08_430275 65 20 AD ad NN 10_1101-2021_02_08_430275 65 21 ) ) -RRB- 10_1101-2021_02_08_430275 65 22 fields field NNS 10_1101-2021_02_08_430275 65 23 ( ( -LRB- 10_1101-2021_02_08_430275 65 24 one one CD 10_1101-2021_02_08_430275 65 25 for for IN 10_1101-2021_02_08_430275 65 26 each each DT 10_1101-2021_02_08_430275 65 27 165 165 CD 10_1101-2021_02_08_430275 65 28 bulk bulk NN 10_1101-2021_02_08_430275 65 29 / / SYM 10_1101-2021_02_08_430275 65 30 parent parent NN 10_1101-2021_02_08_430275 65 31 ) ) -RRB- 10_1101-2021_02_08_430275 65 32 . . . 10_1101-2021_02_08_430275 66 1 This this DT 10_1101-2021_02_08_430275 66 2 step step NN 10_1101-2021_02_08_430275 66 3 is be VBZ 10_1101-2021_02_08_430275 66 4 carried carry VBN 10_1101-2021_02_08_430275 66 5 out out RP 10_1101-2021_02_08_430275 66 6 before before IN 10_1101-2021_02_08_430275 66 7 checking check VBG 10_1101-2021_02_08_430275 66 8 the the DT 10_1101-2021_02_08_430275 66 9 genotype genotype NN 10_1101-2021_02_08_430275 66 10 166 166 CD 10_1101-2021_02_08_430275 66 11 agreement agreement NN 10_1101-2021_02_08_430275 66 12 between between IN 10_1101-2021_02_08_430275 66 13 bulks bulk NNS 10_1101-2021_02_08_430275 66 14 and and CC 10_1101-2021_02_08_430275 66 15 the the DT 10_1101-2021_02_08_430275 66 16 REF REF NNP 10_1101-2021_02_08_430275 66 17 / / SYM 10_1101-2021_02_08_430275 66 18 ALT ALT NNP 10_1101-2021_02_08_430275 66 19 fields field NNS 10_1101-2021_02_08_430275 66 20 . . . 10_1101-2021_02_08_430275 67 1 When when WRB 10_1101-2021_02_08_430275 67 2 parental parental JJ 10_1101-2021_02_08_430275 67 3 167 167 CD 10_1101-2021_02_08_430275 67 4 genome genome JJ 10_1101-2021_02_08_430275 67 5 sequences sequence NNS 10_1101-2021_02_08_430275 67 6 are be VBP 10_1101-2021_02_08_430275 67 7 involved involve VBN 10_1101-2021_02_08_430275 67 8 , , , 10_1101-2021_02_08_430275 67 9 the the DT 10_1101-2021_02_08_430275 67 10 common common JJ 10_1101-2021_02_08_430275 67 11 SNP SNP NNP 10_1101-2021_02_08_430275 67 12 set set NN 10_1101-2021_02_08_430275 67 13 is be VBZ 10_1101-2021_02_08_430275 67 14 identified identify VBN 10_1101-2021_02_08_430275 67 15 168 168 CD 10_1101-2021_02_08_430275 67 16 before before IN 10_1101-2021_02_08_430275 67 17 filtering filter VBG 10_1101-2021_02_08_430275 67 18 out out RP 10_1101-2021_02_08_430275 67 19 the the DT 10_1101-2021_02_08_430275 67 20 SNPs snp NNS 10_1101-2021_02_08_430275 67 21 with with IN 10_1101-2021_02_08_430275 67 22 a a DT 10_1101-2021_02_08_430275 67 23 low low JJ 10_1101-2021_02_08_430275 67 24 GQ GQ NNP 10_1101-2021_02_08_430275 67 25 score score NN 10_1101-2021_02_08_430275 67 26 in in IN 10_1101-2021_02_08_430275 67 27 the the DT 10_1101-2021_02_08_430275 67 28 parental parental JJ 10_1101-2021_02_08_430275 67 29 169 169 CD 10_1101-2021_02_08_430275 67 30 SNP SNP NNP 10_1101-2021_02_08_430275 67 31 dataset dataset NN 10_1101-2021_02_08_430275 67 32 . . . 10_1101-2021_02_08_430275 68 1 170 170 CD 10_1101-2021_02_08_430275 68 2 The the DT 10_1101-2021_02_08_430275 68 3 tightly tightly RB 10_1101-2021_02_08_430275 68 4 linked link VBN 10_1101-2021_02_08_430275 68 5 SNP SNP NNP 10_1101-2021_02_08_430275 68 6 alleles allele NNS 10_1101-2021_02_08_430275 68 7 from from IN 10_1101-2021_02_08_430275 68 8 the the DT 10_1101-2021_02_08_430275 68 9 same same JJ 10_1101-2021_02_08_430275 68 10 parent parent NN 10_1101-2021_02_08_430275 68 11 tend tend VB 10_1101-2021_02_08_430275 68 12 to to IN 10_1101-2021_02_08_430275 68 13 171 171 CD 10_1101-2021_02_08_430275 68 14 segregate segregate NN 10_1101-2021_02_08_430275 68 15 together together RB 10_1101-2021_02_08_430275 68 16 and and CC 10_1101-2021_02_08_430275 68 17 should should MD 10_1101-2021_02_08_430275 68 18 have have VB 10_1101-2021_02_08_430275 68 19 a a DT 10_1101-2021_02_08_430275 68 20 similar similar JJ 10_1101-2021_02_08_430275 68 21 extent extent NN 10_1101-2021_02_08_430275 68 22 of of IN 10_1101-2021_02_08_430275 68 23 allele allele NNP 10_1101-2021_02_08_430275 68 24 enrich- enrich- VBG 10_1101-2021_02_08_430275 68 25 172 172 CD 10_1101-2021_02_08_430275 68 26 ment ment NN 10_1101-2021_02_08_430275 68 27 , , , 10_1101-2021_02_08_430275 68 28 and and CC 10_1101-2021_02_08_430275 68 29 thus thus RB 10_1101-2021_02_08_430275 68 30 similar similar JJ 10_1101-2021_02_08_430275 68 31 AD ad NN 10_1101-2021_02_08_430275 68 32 values value NNS 10_1101-2021_02_08_430275 68 33 . . . 10_1101-2021_02_08_430275 69 1 In in IN 10_1101-2021_02_08_430275 69 2 a a DT 10_1101-2021_02_08_430275 69 3 SNP SNP NNP 10_1101-2021_02_08_430275 69 4 dataset dataset NN 10_1101-2021_02_08_430275 69 5 , , , 10_1101-2021_02_08_430275 69 6 the the DT 10_1101-2021_02_08_430275 69 7 genotypes genotype NNS 10_1101-2021_02_08_430275 69 8 173 173 CD 10_1101-2021_02_08_430275 69 9 of of IN 10_1101-2021_02_08_430275 69 10 each each DT 10_1101-2021_02_08_430275 69 11 bulk bulk NN 10_1101-2021_02_08_430275 69 12 / / SYM 10_1101-2021_02_08_430275 69 13 parent parent NN 10_1101-2021_02_08_430275 69 14 are be VBP 10_1101-2021_02_08_430275 69 15 represented represent VBN 10_1101-2021_02_08_430275 69 16 as as IN 10_1101-2021_02_08_430275 69 17 ‘ ' `` 10_1101-2021_02_08_430275 69 18 GTref GTref NNP 10_1101-2021_02_08_430275 69 19 / / SYM 10_1101-2021_02_08_430275 69 20 GTalt GTalt NNP 10_1101-2021_02_08_430275 69 21 ’ ' '' 10_1101-2021_02_08_430275 69 22 when when WRB 10_1101-2021_02_08_430275 69 23 a a DT 10_1101-2021_02_08_430275 69 24 SNP SNP NNP 10_1101-2021_02_08_430275 69 25 174 174 CD 10_1101-2021_02_08_430275 69 26 contains contain VBZ 10_1101-2021_02_08_430275 69 27 both both CC 10_1101-2021_02_08_430275 69 28 the the DT 10_1101-2021_02_08_430275 69 29 REF REF NNP 10_1101-2021_02_08_430275 69 30 base base NN 10_1101-2021_02_08_430275 69 31 and and CC 10_1101-2021_02_08_430275 69 32 the the DT 10_1101-2021_02_08_430275 69 33 ALT ALT NNP 10_1101-2021_02_08_430275 69 34 base base NN 10_1101-2021_02_08_430275 69 35 in in IN 10_1101-2021_02_08_430275 69 36 the the DT 10_1101-2021_02_08_430275 69 37 genotype genotype NN 10_1101-2021_02_08_430275 69 38 175 175 CD 10_1101-2021_02_08_430275 69 39 ( ( -LRB- 10_1101-2021_02_08_430275 69 40 GT GT NNP 10_1101-2021_02_08_430275 69 41 ) ) -RRB- 10_1101-2021_02_08_430275 69 42 field field NN 10_1101-2021_02_08_430275 69 43 , , , 10_1101-2021_02_08_430275 69 44 and and CC 10_1101-2021_02_08_430275 69 45 the the DT 10_1101-2021_02_08_430275 69 46 AD ad NN 10_1101-2021_02_08_430275 69 47 values value NNS 10_1101-2021_02_08_430275 69 48 in in IN 10_1101-2021_02_08_430275 69 49 each each DT 10_1101-2021_02_08_430275 69 50 bulk bulk NN 10_1101-2021_02_08_430275 69 51 / / SYM 10_1101-2021_02_08_430275 69 52 parent parent NN 10_1101-2021_02_08_430275 69 53 is be VBZ 10_1101-2021_02_08_430275 69 54 represented represent VBN 10_1101-2021_02_08_430275 69 55 as as IN 10_1101-2021_02_08_430275 69 56 176 176 CD 10_1101-2021_02_08_430275 69 57 ‘ ' `` 10_1101-2021_02_08_430275 69 58 ADref ADref NNP 10_1101-2021_02_08_430275 69 59 , , , 10_1101-2021_02_08_430275 69 60 ADalt ADalt NNP 10_1101-2021_02_08_430275 69 61 ’ ' '' 10_1101-2021_02_08_430275 69 62 . . . 10_1101-2021_02_08_430275 70 1 The the DT 10_1101-2021_02_08_430275 70 2 genotype genotype NN 10_1101-2021_02_08_430275 70 3 and and CC 10_1101-2021_02_08_430275 70 4 the the DT 10_1101-2021_02_08_430275 70 5 AD ad NN 10_1101-2021_02_08_430275 70 6 value value NN 10_1101-2021_02_08_430275 70 7 of of IN 10_1101-2021_02_08_430275 70 8 the the DT 10_1101-2021_02_08_430275 70 9 REF REF NNP 10_1101-2021_02_08_430275 70 10 allele allele NNP 10_1101-2021_02_08_430275 70 11 are be VBP 10_1101-2021_02_08_430275 70 12 177 177 CD 10_1101-2021_02_08_430275 70 13 always always RB 10_1101-2021_02_08_430275 70 14 placed place VBN 10_1101-2021_02_08_430275 70 15 first first RB 10_1101-2021_02_08_430275 70 16 in in IN 10_1101-2021_02_08_430275 70 17 both both DT 10_1101-2021_02_08_430275 70 18 fields field NNS 10_1101-2021_02_08_430275 70 19 . . . 10_1101-2021_02_08_430275 71 1 For for IN 10_1101-2021_02_08_430275 71 2 a a DT 10_1101-2021_02_08_430275 71 3 SNP SNP NNP 10_1101-2021_02_08_430275 71 4 locus locus NN 10_1101-2021_02_08_430275 71 5 in in IN 10_1101-2021_02_08_430275 71 6 the the DT 10_1101-2021_02_08_430275 71 7 .tsv .tsv CD 10_1101-2021_02_08_430275 71 8 input input NN 10_1101-2021_02_08_430275 71 9 178 178 CD 10_1101-2021_02_08_430275 71 10 file file NN 10_1101-2021_02_08_430275 71 11 , , , 10_1101-2021_02_08_430275 71 12 the the DT 10_1101-2021_02_08_430275 71 13 allele allele NNP 10_1101-2021_02_08_430275 71 14 having have VBG 10_1101-2021_02_08_430275 71 15 the the DT 10_1101-2021_02_08_430275 71 16 same same JJ 10_1101-2021_02_08_430275 71 17 genotype genotype NN 10_1101-2021_02_08_430275 71 18 as as IN 10_1101-2021_02_08_430275 71 19 that that DT 10_1101-2021_02_08_430275 71 20 in in IN 10_1101-2021_02_08_430275 71 21 the the DT 10_1101-2021_02_08_430275 71 22 reference reference NN 10_1101-2021_02_08_430275 71 23 179 179 CD 10_1101-2021_02_08_430275 71 24 genome genome NN 10_1101-2021_02_08_430275 71 25 is be VBZ 10_1101-2021_02_08_430275 71 26 defined define VBN 10_1101-2021_02_08_430275 71 27 as as IN 10_1101-2021_02_08_430275 71 28 the the DT 10_1101-2021_02_08_430275 71 29 REF REF NNP 10_1101-2021_02_08_430275 71 30 allele allele NNP 10_1101-2021_02_08_430275 71 31 . . . 10_1101-2021_02_08_430275 72 1 However however RB 10_1101-2021_02_08_430275 72 2 , , , 10_1101-2021_02_08_430275 72 3 it -PRON- PRP 10_1101-2021_02_08_430275 72 4 is be VBZ 10_1101-2021_02_08_430275 72 5 highly highly RB 10_1101-2021_02_08_430275 72 6 unlikely unlikely JJ 10_1101-2021_02_08_430275 72 7 180 180 CD 10_1101-2021_02_08_430275 72 8 that that IN 10_1101-2021_02_08_430275 72 9 all all DT 10_1101-2021_02_08_430275 72 10 of of IN 10_1101-2021_02_08_430275 72 11 the the DT 10_1101-2021_02_08_430275 72 12 SNP SNP NNP 10_1101-2021_02_08_430275 72 13 alleles allele NNS 10_1101-2021_02_08_430275 72 14 in in IN 10_1101-2021_02_08_430275 72 15 a a DT 10_1101-2021_02_08_430275 72 16 parent parent NN 10_1101-2021_02_08_430275 72 17 are be VBP 10_1101-2021_02_08_430275 72 18 the the DT 10_1101-2021_02_08_430275 72 19 same same JJ 10_1101-2021_02_08_430275 72 20 as as IN 10_1101-2021_02_08_430275 72 21 those those DT 10_1101-2021_02_08_430275 72 22 in in IN 10_1101-2021_02_08_430275 72 23 181 181 CD 10_1101-2021_02_08_430275 72 24 the the DT 10_1101-2021_02_08_430275 72 25 reference reference NN 10_1101-2021_02_08_430275 72 26 genome genome NN 10_1101-2021_02_08_430275 72 27 , , , 10_1101-2021_02_08_430275 72 28 except except IN 10_1101-2021_02_08_430275 72 29 in in IN 10_1101-2021_02_08_430275 72 30 instances instance NNS 10_1101-2021_02_08_430275 72 31 where where WRB 10_1101-2021_02_08_430275 72 32 reference reference NN 10_1101-2021_02_08_430275 72 33 genome genome NN 10_1101-2021_02_08_430275 72 34 182 182 CD 10_1101-2021_02_08_430275 72 35 sequences sequence NNS 10_1101-2021_02_08_430275 72 36 used use VBN 10_1101-2021_02_08_430275 72 37 in in IN 10_1101-2021_02_08_430275 72 38 SNP SNP NNP 10_1101-2021_02_08_430275 72 39 calling call VBG 10_1101-2021_02_08_430275 72 40 are be VBP 10_1101-2021_02_08_430275 72 41 from from IN 10_1101-2021_02_08_430275 72 42 one one CD 10_1101-2021_02_08_430275 72 43 of of IN 10_1101-2021_02_08_430275 72 44 the the DT 10_1101-2021_02_08_430275 72 45 parents parent NNS 10_1101-2021_02_08_430275 72 46 as as IN 10_1101-2021_02_08_430275 72 47 in in IN 10_1101-2021_02_08_430275 72 48 183 183 CD 10_1101-2021_02_08_430275 72 49 the the DT 10_1101-2021_02_08_430275 72 50 case case NN 10_1101-2021_02_08_430275 72 51 of of IN 10_1101-2021_02_08_430275 72 52 the the DT 10_1101-2021_02_08_430275 72 53 cold cold JJ 10_1101-2021_02_08_430275 72 54 - - HYPH 10_1101-2021_02_08_430275 72 55 tolerance tolerance NN 10_1101-2021_02_08_430275 72 56 study study NN 10_1101-2021_02_08_430275 72 57 as as IN 10_1101-2021_02_08_430275 72 58 mentioned mention VBN 10_1101-2021_02_08_430275 72 59 above above RB 10_1101-2021_02_08_430275 72 60 ( ( -LRB- 10_1101-2021_02_08_430275 72 61 9 9 CD 10_1101-2021_02_08_430275 72 62 ) ) -RRB- 10_1101-2021_02_08_430275 72 63 . . . 10_1101-2021_02_08_430275 73 1 It -PRON- PRP 10_1101-2021_02_08_430275 73 2 is be VBZ 10_1101-2021_02_08_430275 73 3 184 184 CD 10_1101-2021_02_08_430275 73 4 necessary necessary JJ 10_1101-2021_02_08_430275 73 5 to to TO 10_1101-2021_02_08_430275 73 6 place place VB 10_1101-2021_02_08_430275 73 7 the the DT 10_1101-2021_02_08_430275 73 8 genotypes genotype NNS 10_1101-2021_02_08_430275 73 9 and and CC 10_1101-2021_02_08_430275 73 10 AD ad NN 10_1101-2021_02_08_430275 73 11 values value NNS 10_1101-2021_02_08_430275 73 12 of of IN 10_1101-2021_02_08_430275 73 13 all all DT 10_1101-2021_02_08_430275 73 14 SNP SNP NNP 10_1101-2021_02_08_430275 73 15 alleles allele VBZ 10_1101-2021_02_08_430275 73 16 185 185 CD 10_1101-2021_02_08_430275 73 17 from from IN 10_1101-2021_02_08_430275 73 18 one one CD 10_1101-2021_02_08_430275 73 19 parent parent NN 10_1101-2021_02_08_430275 73 20 ( ( -LRB- 10_1101-2021_02_08_430275 73 21 e.g. e.g. RB 10_1101-2021_02_08_430275 73 22 , , , 10_1101-2021_02_08_430275 73 23 LD24 LD24 NNP 10_1101-2021_02_08_430275 73 24 ) ) -RRB- 10_1101-2021_02_08_430275 73 25 in in IN 10_1101-2021_02_08_430275 73 26 the the DT 10_1101-2021_02_08_430275 73 27 REF REF NNP 10_1101-2021_02_08_430275 73 28 position position NN 10_1101-2021_02_08_430275 73 29 , , , 10_1101-2021_02_08_430275 73 30 and and CC 10_1101-2021_02_08_430275 73 31 those those DT 10_1101-2021_02_08_430275 73 32 from from IN 10_1101-2021_02_08_430275 73 33 186 186 CD 10_1101-2021_02_08_430275 73 34 the the DT 10_1101-2021_02_08_430275 73 35 other other JJ 10_1101-2021_02_08_430275 73 36 parent parent NN 10_1101-2021_02_08_430275 73 37 ( ( -LRB- 10_1101-2021_02_08_430275 73 38 e.g. e.g. RB 10_1101-2021_02_08_430275 73 39 , , , 10_1101-2021_02_08_430275 73 40 VialoneNano VialoneNano NNP 10_1101-2021_02_08_430275 73 41 ) ) -RRB- 10_1101-2021_02_08_430275 73 42 to to IN 10_1101-2021_02_08_430275 73 43 the the DT 10_1101-2021_02_08_430275 73 44 ALT ALT NNP 10_1101-2021_02_08_430275 73 45 position position NN 10_1101-2021_02_08_430275 73 46 in in IN 10_1101-2021_02_08_430275 73 47 the the DT 10_1101-2021_02_08_430275 73 48 187 187 CD 10_1101-2021_02_08_430275 73 49 GT GT NNP 10_1101-2021_02_08_430275 73 50 and and CC 10_1101-2021_02_08_430275 73 51 AD ad NN 10_1101-2021_02_08_430275 73 52 fields field NNS 10_1101-2021_02_08_430275 73 53 to to TO 10_1101-2021_02_08_430275 73 54 make make VB 10_1101-2021_02_08_430275 73 55 the the DT 10_1101-2021_02_08_430275 73 56 bulk bulk JJ 10_1101-2021_02_08_430275 73 57 dataset dataset NN 10_1101-2021_02_08_430275 73 58 consistent consistent NNP 10_1101-2021_02_08_430275 73 59 . . . 10_1101-2021_02_08_430275 74 1 Thus thus RB 10_1101-2021_02_08_430275 74 2 , , , 10_1101-2021_02_08_430275 74 3 for for IN 10_1101-2021_02_08_430275 74 4 188 188 CD 10_1101-2021_02_08_430275 74 5 a a DT 10_1101-2021_02_08_430275 74 6 particular particular JJ 10_1101-2021_02_08_430275 74 7 SNP snp NN 10_1101-2021_02_08_430275 74 8 , , , 10_1101-2021_02_08_430275 74 9 if if IN 10_1101-2021_02_08_430275 74 10 the the DT 10_1101-2021_02_08_430275 74 11 REF REF NNP 10_1101-2021_02_08_430275 74 12 base base NN 10_1101-2021_02_08_430275 74 13 in in IN 10_1101-2021_02_08_430275 74 14 the the DT 10_1101-2021_02_08_430275 74 15 .tsv .tsv CD 10_1101-2021_02_08_430275 74 16 file file NN 10_1101-2021_02_08_430275 74 17 is be VBZ 10_1101-2021_02_08_430275 74 18 different different JJ 10_1101-2021_02_08_430275 74 19 from from IN 10_1101-2021_02_08_430275 74 20 189 189 CD 10_1101-2021_02_08_430275 74 21 the the DT 10_1101-2021_02_08_430275 74 22 genotype genotype NN 10_1101-2021_02_08_430275 74 23 of of IN 10_1101-2021_02_08_430275 74 24 LD24 LD24 NNP 10_1101-2021_02_08_430275 74 25 ( ( -LRB- 10_1101-2021_02_08_430275 74 26 either either CC 10_1101-2021_02_08_430275 74 27 parent parent NN 10_1101-2021_02_08_430275 74 28 will will MD 10_1101-2021_02_08_430275 74 29 work work VB 10_1101-2021_02_08_430275 74 30 ) ) -RRB- 10_1101-2021_02_08_430275 74 31 , , , 10_1101-2021_02_08_430275 74 32 its -PRON- PRP$ 10_1101-2021_02_08_430275 74 33 GT GT NNP 10_1101-2021_02_08_430275 74 34 / / , 10_1101-2021_02_08_430275 74 35 AD ad NN 10_1101-2021_02_08_430275 74 36 values value NNS 10_1101-2021_02_08_430275 74 37 190 190 CD 10_1101-2021_02_08_430275 74 38 would would MD 10_1101-2021_02_08_430275 74 39 be be VB 10_1101-2021_02_08_430275 74 40 swapped swap VBN 10_1101-2021_02_08_430275 74 41 , , , 10_1101-2021_02_08_430275 74 42 e.g. e.g. RB 10_1101-2021_02_08_430275 74 43 , , , 10_1101-2021_02_08_430275 74 44 ‘ ' `` 10_1101-2021_02_08_430275 74 45 G g NN 10_1101-2021_02_08_430275 74 46 / / , 10_1101-2021_02_08_430275 74 47 A A NNP 10_1101-2021_02_08_430275 74 48 ’ ' '' 10_1101-2021_02_08_430275 74 49 to to TO 10_1101-2021_02_08_430275 74 50 ‘ ' `` 10_1101-2021_02_08_430275 74 51 A a NN 10_1101-2021_02_08_430275 74 52 / / SYM 10_1101-2021_02_08_430275 74 53 G g NN 10_1101-2021_02_08_430275 74 54 ’ ' '' 10_1101-2021_02_08_430275 74 55 and and CC 10_1101-2021_02_08_430275 74 56 ‘ ' `` 10_1101-2021_02_08_430275 74 57 19,9 19,9 NNS 10_1101-2021_02_08_430275 74 58 ’ ' '' 10_1101-2021_02_08_430275 74 59 to to IN 10_1101-2021_02_08_430275 74 60 ‘ ' `` 10_1101-2021_02_08_430275 74 61 9,19 9,19 CD 10_1101-2021_02_08_430275 74 62 ’ ' '' 10_1101-2021_02_08_430275 74 63 . . . 10_1101-2021_02_08_430275 75 1 AD ad NN 10_1101-2021_02_08_430275 75 2 / / SYM 10_1101-2021_02_08_430275 75 3 GT GT NNP 10_1101-2021_02_08_430275 75 4 191 191 CD 10_1101-2021_02_08_430275 75 5 swapping swap VBG 10_1101-2021_02_08_430275 75 6 is be VBZ 10_1101-2021_02_08_430275 75 7 performed perform VBN 10_1101-2021_02_08_430275 75 8 following follow VBG 10_1101-2021_02_08_430275 75 9 SNP SNP NNP 10_1101-2021_02_08_430275 75 10 filtering filtering NN 10_1101-2021_02_08_430275 75 11 and and CC 10_1101-2021_02_08_430275 75 12 is be VBZ 10_1101-2021_02_08_430275 75 13 performed perform VBN 10_1101-2021_02_08_430275 75 14 only only RB 10_1101-2021_02_08_430275 75 15 192 192 CD 10_1101-2021_02_08_430275 75 16 when when WRB 10_1101-2021_02_08_430275 75 17 the the DT 10_1101-2021_02_08_430275 75 18 parental parental JJ 10_1101-2021_02_08_430275 75 19 genome genome NN 10_1101-2021_02_08_430275 75 20 sequences sequence NNS 10_1101-2021_02_08_430275 75 21 are be VBP 10_1101-2021_02_08_430275 75 22 used use VBN 10_1101-2021_02_08_430275 75 23 to to TO 10_1101-2021_02_08_430275 75 24 aid aid VB 10_1101-2021_02_08_430275 75 25 BSA BSA NNP 10_1101-2021_02_08_430275 75 26 - - HYPH 10_1101-2021_02_08_430275 75 27 Seq Seq NNP 10_1101-2021_02_08_430275 75 28 data datum NNS 10_1101-2021_02_08_430275 75 29 193 193 CD 10_1101-2021_02_08_430275 75 30 analysis analysis NN 10_1101-2021_02_08_430275 75 31 . . . 10_1101-2021_02_08_430275 76 1 Equation equation NN 10_1101-2021_02_08_430275 76 2 1 1 CD 10_1101-2021_02_08_430275 76 3 is be VBZ 10_1101-2021_02_08_430275 76 4 used use VBN 10_1101-2021_02_08_430275 76 5 for for IN 10_1101-2021_02_08_430275 76 6 ΔAF δaf NN 10_1101-2021_02_08_430275 76 7 calculation calculation NN 10_1101-2021_02_08_430275 76 8 . . . 10_1101-2021_02_08_430275 77 1 AD ad NN 10_1101-2021_02_08_430275 77 2 swapping swap VBG 10_1101-2021_02_08_430275 77 3 194 194 CD 10_1101-2021_02_08_430275 77 4 ensures ensure NNS 10_1101-2021_02_08_430275 77 5 that that IN 10_1101-2021_02_08_430275 77 6 adjacent adjacent JJ 10_1101-2021_02_08_430275 77 7 SNPs snp NNS 10_1101-2021_02_08_430275 77 8 have have VBP 10_1101-2021_02_08_430275 77 9 similar similar JJ 10_1101-2021_02_08_430275 77 10 ΔAF δaf NN 10_1101-2021_02_08_430275 77 11 values value NNS 10_1101-2021_02_08_430275 77 12 . . . 10_1101-2021_02_08_430275 78 1 195 195 CD 10_1101-2021_02_08_430275 78 2 ∆AF ∆AF NNP 10_1101-2021_02_08_430275 78 3 = = SYM 10_1101-2021_02_08_430275 78 4 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 78 5 ADref ADref NNP 10_1101-2021_02_08_430275 78 6 2 2 CD 10_1101-2021_02_08_430275 78 7 + + NNP 10_1101-2021_02_08_430275 78 8 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 78 9 − − NNP 10_1101-2021_02_08_430275 78 10 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 78 11 ADref ADref NNP 10_1101-2021_02_08_430275 78 12 1 1 CD 10_1101-2021_02_08_430275 78 13 + + SYM 10_1101-2021_02_08_430275 78 14 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 78 15 [ [ -LRB- 10_1101-2021_02_08_430275 78 16 1 1 CD 10_1101-2021_02_08_430275 78 17 ] ] -RRB- 10_1101-2021_02_08_430275 78 18 196 196 CD 10_1101-2021_02_08_430275 78 19 197 197 CD 10_1101-2021_02_08_430275 78 20 Results result NNS 10_1101-2021_02_08_430275 78 21 198 198 CD 10_1101-2021_02_08_430275 78 22 The the DT 10_1101-2021_02_08_430275 78 23 original original JJ 10_1101-2021_02_08_430275 78 24 sequence sequence NN 10_1101-2021_02_08_430275 78 25 reads read VBZ 10_1101-2021_02_08_430275 78 26 were be VBD 10_1101-2021_02_08_430275 78 27 3.9 3.9 CD 10_1101-2021_02_08_430275 78 28 G g NN 10_1101-2021_02_08_430275 78 29 , , , 10_1101-2021_02_08_430275 78 30 3.8 3.8 CD 10_1101-2021_02_08_430275 78 31 G g NN 10_1101-2021_02_08_430275 78 32 , , , 10_1101-2021_02_08_430275 78 33 3.4 3.4 CD 10_1101-2021_02_08_430275 78 34 G g NN 10_1101-2021_02_08_430275 78 35 , , , 10_1101-2021_02_08_430275 78 36 and and CC 10_1101-2021_02_08_430275 78 37 3.5 3.5 CD 10_1101-2021_02_08_430275 78 38 G g NN 10_1101-2021_02_08_430275 78 39 ; ; : 10_1101-2021_02_08_430275 78 40 199 199 CD 10_1101-2021_02_08_430275 78 41 they -PRON- PRP 10_1101-2021_02_08_430275 78 42 became become VBD 10_1101-2021_02_08_430275 78 43 3.8 3.8 CD 10_1101-2021_02_08_430275 78 44 G g NN 10_1101-2021_02_08_430275 78 45 , , , 10_1101-2021_02_08_430275 78 46 3.6 3.6 CD 10_1101-2021_02_08_430275 78 47 G g NN 10_1101-2021_02_08_430275 78 48 , , , 10_1101-2021_02_08_430275 78 49 3.3 3.3 CD 10_1101-2021_02_08_430275 78 50 G g NN 10_1101-2021_02_08_430275 78 51 , , , 10_1101-2021_02_08_430275 78 52 and and CC 10_1101-2021_02_08_430275 78 53 3.4 3.4 CD 10_1101-2021_02_08_430275 78 54 G g NN 10_1101-2021_02_08_430275 78 55 after after IN 10_1101-2021_02_08_430275 78 56 quality quality NN 10_1101-2021_02_08_430275 78 57 con- con- NN 10_1101-2021_02_08_430275 78 58 200 200 CD 10_1101-2021_02_08_430275 78 59 trol trol NN 10_1101-2021_02_08_430275 78 60 , , , 10_1101-2021_02_08_430275 78 61 respectively respectively RB 10_1101-2021_02_08_430275 78 62 , , , 10_1101-2021_02_08_430275 78 63 in in IN 10_1101-2021_02_08_430275 78 64 ERR2696318 ERR2696318 NNP 10_1101-2021_02_08_430275 78 65 ( ( -LRB- 10_1101-2021_02_08_430275 78 66 parent parent NN 10_1101-2021_02_08_430275 78 67 LD24 LD24 NNP 10_1101-2021_02_08_430275 78 68 ) ) -RRB- 10_1101-2021_02_08_430275 78 69 , , , 10_1101-2021_02_08_430275 78 70 ERR2696319 ERR2696319 NNP 10_1101-2021_02_08_430275 78 71 201 201 CD 10_1101-2021_02_08_430275 78 72 ( ( -LRB- 10_1101-2021_02_08_430275 78 73 parent parent NN 10_1101-2021_02_08_430275 78 74 VialoneNano VialoneNano NNP 10_1101-2021_02_08_430275 78 75 ) ) -RRB- 10_1101-2021_02_08_430275 78 76 , , , 10_1101-2021_02_08_430275 78 77 ERR2696321 ERR2696321 NNP 10_1101-2021_02_08_430275 78 78 ( ( -LRB- 10_1101-2021_02_08_430275 78 79 the the DT 10_1101-2021_02_08_430275 78 80 resistant resistant JJ 10_1101-2021_02_08_430275 78 81 bulk bulk NN 10_1101-2021_02_08_430275 78 82 ) ) -RRB- 10_1101-2021_02_08_430275 78 83 , , , 10_1101-2021_02_08_430275 78 84 and and CC 10_1101-2021_02_08_430275 78 85 202 202 CD 10_1101-2021_02_08_430275 78 86 ERR2696322 ERR2696322 NNP 10_1101-2021_02_08_430275 78 87 ( ( -LRB- 10_1101-2021_02_08_430275 78 88 the the DT 10_1101-2021_02_08_430275 78 89 susceptible susceptible JJ 10_1101-2021_02_08_430275 78 90 bulk bulk NN 10_1101-2021_02_08_430275 78 91 ) ) -RRB- 10_1101-2021_02_08_430275 78 92 , , , 10_1101-2021_02_08_430275 78 93 which which WDT 10_1101-2021_02_08_430275 78 94 correspond correspond VBP 10_1101-2021_02_08_430275 78 95 to to IN 10_1101-2021_02_08_430275 78 96 8.8× 8.8× CD 10_1101-2021_02_08_430275 78 97 , , , 10_1101-2021_02_08_430275 78 98 203 203 CD 10_1101-2021_02_08_430275 78 99 8.5× 8.5× CD 10_1101-2021_02_08_430275 78 100 , , , 10_1101-2021_02_08_430275 78 101 7.6× 7.6× CD 10_1101-2021_02_08_430275 78 102 , , , 10_1101-2021_02_08_430275 78 103 and and CC 10_1101-2021_02_08_430275 78 104 7.9× 7.9× CD 10_1101-2021_02_08_430275 78 105 coverage coverage NN 10_1101-2021_02_08_430275 78 106 , , , 10_1101-2021_02_08_430275 78 107 respectively respectively RB 10_1101-2021_02_08_430275 78 108 ( ( -LRB- 10_1101-2021_02_08_430275 78 109 12 12 CD 10_1101-2021_02_08_430275 78 110 ) ) -RRB- 10_1101-2021_02_08_430275 78 111 . . . 10_1101-2021_02_08_430275 79 1 The the DT 10_1101-2021_02_08_430275 79 2 prepro- prepro- NN 10_1101-2021_02_08_430275 79 3 204 204 CD 10_1101-2021_02_08_430275 79 4 cessed cessed JJ 10_1101-2021_02_08_430275 79 5 sequences sequence NNS 10_1101-2021_02_08_430275 79 6 were be VBD 10_1101-2021_02_08_430275 79 7 used use VBN 10_1101-2021_02_08_430275 79 8 for for IN 10_1101-2021_02_08_430275 79 9 SNP SNP NNP 10_1101-2021_02_08_430275 79 10 calling call VBG 10_1101-2021_02_08_430275 79 11 to to TO 10_1101-2021_02_08_430275 79 12 generate generate VB 10_1101-2021_02_08_430275 79 13 a a DT 10_1101-2021_02_08_430275 79 14 SNP SNP NNP 10_1101-2021_02_08_430275 79 15 205 205 CD 10_1101-2021_02_08_430275 79 16 2 2 CD 10_1101-2021_02_08_430275 79 17 | | NNP 10_1101-2021_02_08_430275 79 18 https://doi.org/10.1101/654137 https://doi.org/10.1101/654137 NN 10_1101-2021_02_08_430275 79 19 Zhang Zhang NNP 10_1101-2021_02_08_430275 79 20 et et NNP 10_1101-2021_02_08_430275 79 21 al al NNP 10_1101-2021_02_08_430275 79 22 . . . 10_1101-2021_02_08_430275 80 1 .CC .CC NFP 10_1101-2021_02_08_430275 80 2 - - : 10_1101-2021_02_08_430275 80 3 BY by IN 10_1101-2021_02_08_430275 80 4 - - HYPH 10_1101-2021_02_08_430275 80 5 NC NC NNP 10_1101-2021_02_08_430275 80 6 - - HYPH 10_1101-2021_02_08_430275 80 7 ND ND NNP 10_1101-2021_02_08_430275 80 8 4.0 4.0 CD 10_1101-2021_02_08_430275 80 9 International International NNP 10_1101-2021_02_08_430275 80 10 licenseavailable licenseavailable NN 10_1101-2021_02_08_430275 80 11 under under IN 10_1101-2021_02_08_430275 80 12 a a DT 10_1101-2021_02_08_430275 80 13 ( ( -LRB- 10_1101-2021_02_08_430275 80 14 which which WDT 10_1101-2021_02_08_430275 80 15 was be VBD 10_1101-2021_02_08_430275 80 16 not not RB 10_1101-2021_02_08_430275 80 17 certified certify VBN 10_1101-2021_02_08_430275 80 18 by by IN 10_1101-2021_02_08_430275 80 19 peer peer NN 10_1101-2021_02_08_430275 80 20 review review NN 10_1101-2021_02_08_430275 80 21 ) ) -RRB- 10_1101-2021_02_08_430275 80 22 is be VBZ 10_1101-2021_02_08_430275 80 23 the the DT 10_1101-2021_02_08_430275 80 24 author author NN 10_1101-2021_02_08_430275 80 25 / / SYM 10_1101-2021_02_08_430275 80 26 funder funder NN 10_1101-2021_02_08_430275 80 27 , , , 10_1101-2021_02_08_430275 80 28 who who WP 10_1101-2021_02_08_430275 80 29 has have VBZ 10_1101-2021_02_08_430275 80 30 granted grant VBN 10_1101-2021_02_08_430275 80 31 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 80 32 a a DT 10_1101-2021_02_08_430275 80 33 license license NN 10_1101-2021_02_08_430275 80 34 to to TO 10_1101-2021_02_08_430275 80 35 display display VB 10_1101-2021_02_08_430275 80 36 the the DT 10_1101-2021_02_08_430275 80 37 preprint preprint NN 10_1101-2021_02_08_430275 80 38 in in IN 10_1101-2021_02_08_430275 80 39 perpetuity perpetuity NN 10_1101-2021_02_08_430275 80 40 . . . 10_1101-2021_02_08_430275 81 1 It -PRON- PRP 10_1101-2021_02_08_430275 81 2 is be VBZ 10_1101-2021_02_08_430275 81 3 made make VBN 10_1101-2021_02_08_430275 81 4 The the DT 10_1101-2021_02_08_430275 81 5 copyright copyright NN 10_1101-2021_02_08_430275 81 6 holder holder NN 10_1101-2021_02_08_430275 81 7 for for IN 10_1101-2021_02_08_430275 81 8 this this DT 10_1101-2021_02_08_430275 81 9 preprintthis preprintthis NN 10_1101-2021_02_08_430275 81 10 version version NN 10_1101-2021_02_08_430275 81 11 posted post VBD 10_1101-2021_02_08_430275 81 12 February February NNP 10_1101-2021_02_08_430275 81 13 10 10 CD 10_1101-2021_02_08_430275 81 14 , , , 10_1101-2021_02_08_430275 81 15 2021 2021 CD 10_1101-2021_02_08_430275 81 16 . . . 10_1101-2021_02_08_430275 81 17 ; ; : 10_1101-2021_02_08_430275 81 18 https://doi.org/10.1101/2021.02.08.430275doi https://doi.org/10.1101/2021.02.08.430275doi ADD 10_1101-2021_02_08_430275 81 19 : : : 10_1101-2021_02_08_430275 81 20 bioRxiv biorxiv VB 10_1101-2021_02_08_430275 81 21 preprint preprint JJ 10_1101-2021_02_08_430275 81 22 https://plants.ensembl.org/Oryza_sativa/Info/Index https://plants.ensembl.org/oryza_sativa/info/index JJ 10_1101-2021_02_08_430275 81 23 https://plants.ensembl.org/Oryza_sativa/Info/Index https://plants.ensembl.org/Oryza_sativa/Info/Index NNP 10_1101-2021_02_08_430275 81 24 https://plants.ensembl.org/Oryza_sativa/Info/Index https://plants.ensembl.org/Oryza_sativa/Info/Index NNP 10_1101-2021_02_08_430275 81 25 https://github.com/dblhlx/PyBSASeq https://github.com/dblhlx/PyBSASeq NNP 10_1101-2021_02_08_430275 81 26 https://doi.org/10.1101/654137 https://doi.org/10.1101/654137 NNP 10_1101-2021_02_08_430275 81 27 https://doi.org/10.1101/2021.02.08.430275 https://doi.org/10.1101/2021.02.08.430275 NNP 10_1101-2021_02_08_430275 81 28 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_02_08_430275 81 29 DR DR NNP 10_1101-2021_02_08_430275 81 30 AF AF NNP 10_1101-2021_02_08_430275 81 31 T T NNP 10_1101-2021_02_08_430275 81 32 dataset dataset NN 10_1101-2021_02_08_430275 81 33 , , , 10_1101-2021_02_08_430275 81 34 which which WDT 10_1101-2021_02_08_430275 81 35 was be VBD 10_1101-2021_02_08_430275 81 36 analyzed analyze VBN 10_1101-2021_02_08_430275 81 37 using use VBG 10_1101-2021_02_08_430275 81 38 the the DT 10_1101-2021_02_08_430275 81 39 modified modify VBN 10_1101-2021_02_08_430275 81 40 significant206 significant206 NNP 10_1101-2021_02_08_430275 81 41 structural structural JJ 10_1101-2021_02_08_430275 81 42 variant variant JJ 10_1101-2021_02_08_430275 81 43 method method NN 10_1101-2021_02_08_430275 81 44 , , , 10_1101-2021_02_08_430275 81 45 the the DT 10_1101-2021_02_08_430275 81 46 G g NN 10_1101-2021_02_08_430275 81 47 - - HYPH 10_1101-2021_02_08_430275 81 48 statistic statistic NN 10_1101-2021_02_08_430275 81 49 method method NN 10_1101-2021_02_08_430275 81 50 , , , 10_1101-2021_02_08_430275 81 51 and and CC 10_1101-2021_02_08_430275 81 52 the207 the207 CD 10_1101-2021_02_08_430275 81 53 allele allele NNP 10_1101-2021_02_08_430275 81 54 frequency frequency NN 10_1101-2021_02_08_430275 81 55 method method NN 10_1101-2021_02_08_430275 81 56 with with IN 10_1101-2021_02_08_430275 81 57 or or CC 10_1101-2021_02_08_430275 81 58 without without IN 10_1101-2021_02_08_430275 81 59 the the DT 10_1101-2021_02_08_430275 81 60 aid aid NN 10_1101-2021_02_08_430275 81 61 of of IN 10_1101-2021_02_08_430275 81 62 the the DT 10_1101-2021_02_08_430275 81 63 parental208 parental208 NNP 10_1101-2021_02_08_430275 81 64 genome genome NNP 10_1101-2021_02_08_430275 81 65 sequences.209 sequences.209 IN 10_1101-2021_02_08_430275 81 66 BSA BSA NNP 10_1101-2021_02_08_430275 81 67 - - HYPH 10_1101-2021_02_08_430275 81 68 Seq seq NN 10_1101-2021_02_08_430275 81 69 data data NN 10_1101-2021_02_08_430275 81 70 analysis analysis NN 10_1101-2021_02_08_430275 81 71 using use VBG 10_1101-2021_02_08_430275 81 72 the the DT 10_1101-2021_02_08_430275 81 73 genome genome NNP 10_1101-2021_02_08_430275 81 74 sequences sequence NNS 10_1101-2021_02_08_430275 81 75 of210 of210 NN 10_1101-2021_02_08_430275 81 76 both both CC 10_1101-2021_02_08_430275 81 77 the the DT 10_1101-2021_02_08_430275 81 78 parents parent NNS 10_1101-2021_02_08_430275 81 79 and and CC 10_1101-2021_02_08_430275 81 80 the the DT 10_1101-2021_02_08_430275 81 81 bulks bulk NNS 10_1101-2021_02_08_430275 81 82 . . . 10_1101-2021_02_08_430275 82 1 The the DT 10_1101-2021_02_08_430275 82 2 SNP SNP NNP 10_1101-2021_02_08_430275 82 3 calling call VBG 10_1101-2021_02_08_430275 82 4 - - HYPH 10_1101-2021_02_08_430275 82 5 generated211 generated211 NNP 10_1101-2021_02_08_430275 82 6 parent parent NN 10_1101-2021_02_08_430275 82 7 / / SYM 10_1101-2021_02_08_430275 82 8 bulk bulk NNP 10_1101-2021_02_08_430275 82 9 SNP SNP NNP 10_1101-2021_02_08_430275 82 10 dataset dataset NN 10_1101-2021_02_08_430275 82 11 was be VBD 10_1101-2021_02_08_430275 82 12 processed process VBN 10_1101-2021_02_08_430275 82 13 with with IN 10_1101-2021_02_08_430275 82 14 the the DT 10_1101-2021_02_08_430275 82 15 Python212 Python212 NNP 10_1101-2021_02_08_430275 82 16 script script NN 10_1101-2021_02_08_430275 82 17 PyBSASeq_WP.py pybsaseq_wp.py NN 10_1101-2021_02_08_430275 82 18 . . . 10_1101-2021_02_08_430275 83 1 SNP SNP NNP 10_1101-2021_02_08_430275 83 2 filtering filtering NN 10_1101-2021_02_08_430275 83 3 was be VBD 10_1101-2021_02_08_430275 83 4 performed perform VBN 10_1101-2021_02_08_430275 83 5 as213 as213 NN 10_1101-2021_02_08_430275 83 6 described describe VBN 10_1101-2021_02_08_430275 83 7 in in IN 10_1101-2021_02_08_430275 83 8 the the DT 10_1101-2021_02_08_430275 83 9 Materials Materials NNPS 10_1101-2021_02_08_430275 83 10 and and CC 10_1101-2021_02_08_430275 83 11 Methods Methods NNPS 10_1101-2021_02_08_430275 83 12 section section NN 10_1101-2021_02_08_430275 83 13 . . . 10_1101-2021_02_08_430275 84 1 The the DT 10_1101-2021_02_08_430275 84 2 parental214 parental214 NNP 10_1101-2021_02_08_430275 84 3 SNP SNP NNP 10_1101-2021_02_08_430275 84 4 dataset dataset NN 10_1101-2021_02_08_430275 84 5 was be VBD 10_1101-2021_02_08_430275 84 6 processed process VBN 10_1101-2021_02_08_430275 84 7 first first RB 10_1101-2021_02_08_430275 84 8 , , , 10_1101-2021_02_08_430275 84 9 and and CC 10_1101-2021_02_08_430275 84 10 the the DT 10_1101-2021_02_08_430275 84 11 SNPs SNPs NNPS 10_1101-2021_02_08_430275 84 12 heterozygous215 heterozygous215 NNP 10_1101-2021_02_08_430275 84 13 in in IN 10_1101-2021_02_08_430275 84 14 any any DT 10_1101-2021_02_08_430275 84 15 parent parent NN 10_1101-2021_02_08_430275 84 16 were be VBD 10_1101-2021_02_08_430275 84 17 eliminated eliminate VBN 10_1101-2021_02_08_430275 84 18 because because IN 10_1101-2021_02_08_430275 84 19 all all DT 10_1101-2021_02_08_430275 84 20 algorithms algorithm NNS 10_1101-2021_02_08_430275 84 21 assume216 assume216 NNP 10_1101-2021_02_08_430275 84 22 all all DT 10_1101-2021_02_08_430275 84 23 SNP SNP NNP 10_1101-2021_02_08_430275 84 24 loci loci NN 10_1101-2021_02_08_430275 84 25 are be VBP 10_1101-2021_02_08_430275 84 26 homozygous homozygous JJ 10_1101-2021_02_08_430275 84 27 in in IN 10_1101-2021_02_08_430275 84 28 the the DT 10_1101-2021_02_08_430275 84 29 parental parental JJ 10_1101-2021_02_08_430275 84 30 lines line NNS 10_1101-2021_02_08_430275 84 31 . . . 10_1101-2021_02_08_430275 85 1 Threshold217 threshold217 NN 10_1101-2021_02_08_430275 85 2 estimation estimation NN 10_1101-2021_02_08_430275 85 3 is be VBZ 10_1101-2021_02_08_430275 85 4 based base VBN 10_1101-2021_02_08_430275 85 5 on on IN 10_1101-2021_02_08_430275 85 6 this this DT 10_1101-2021_02_08_430275 85 7 assumption assumption NN 10_1101-2021_02_08_430275 85 8 . . . 10_1101-2021_02_08_430275 86 1 Although although IN 10_1101-2021_02_08_430275 86 2 most most JJS 10_1101-2021_02_08_430275 86 3 rice218 rice218 NNP 10_1101-2021_02_08_430275 86 4 breeding breeding NN 10_1101-2021_02_08_430275 86 5 lines line NNS 10_1101-2021_02_08_430275 86 6 should should MD 10_1101-2021_02_08_430275 86 7 be be VB 10_1101-2021_02_08_430275 86 8 homozygous homozygous JJ 10_1101-2021_02_08_430275 86 9 in in IN 10_1101-2021_02_08_430275 86 10 most most JJS 10_1101-2021_02_08_430275 86 11 loci loci NN 10_1101-2021_02_08_430275 86 12 , , , 10_1101-2021_02_08_430275 86 13 more219 more219 NNP 10_1101-2021_02_08_430275 86 14 than than IN 10_1101-2021_02_08_430275 86 15 7 7 CD 10_1101-2021_02_08_430275 86 16 % % NN 10_1101-2021_02_08_430275 86 17 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 86 18 SNP SNP NNP 10_1101-2021_02_08_430275 86 19 loci loci NN 10_1101-2021_02_08_430275 86 20 ( ( -LRB- 10_1101-2021_02_08_430275 86 21 2 2 CD 10_1101-2021_02_08_430275 86 22 011 011 CD 10_1101-2021_02_08_430275 86 23 062 062 CD 10_1101-2021_02_08_430275 86 24 homozygous homozygous JJ 10_1101-2021_02_08_430275 86 25 and220 and220 NNP 10_1101-2021_02_08_430275 86 26 153 153 CD 10_1101-2021_02_08_430275 86 27 000 000 CD 10_1101-2021_02_08_430275 86 28 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 86 29 ) ) -RRB- 10_1101-2021_02_08_430275 86 30 were be VBD 10_1101-2021_02_08_430275 86 31 identified identify VBN 10_1101-2021_02_08_430275 86 32 in in IN 10_1101-2021_02_08_430275 86 33 the the DT 10_1101-2021_02_08_430275 86 34 parental parental JJ 10_1101-2021_02_08_430275 86 35 SNP221 SNP221 NNP 10_1101-2021_02_08_430275 86 36 dataset dataset NN 10_1101-2021_02_08_430275 86 37 . . . 10_1101-2021_02_08_430275 87 1 However however RB 10_1101-2021_02_08_430275 87 2 , , , 10_1101-2021_02_08_430275 87 3 the the DT 10_1101-2021_02_08_430275 87 4 GATK GATK NNP 10_1101-2021_02_08_430275 87 5 ’s ’s POS 10_1101-2021_02_08_430275 87 6 variant variant JJ 10_1101-2021_02_08_430275 87 7 calling calling NN 10_1101-2021_02_08_430275 87 8 tools tool NNS 10_1101-2021_02_08_430275 87 9 are222 are222 CD 10_1101-2021_02_08_430275 87 10 designed design VBN 10_1101-2021_02_08_430275 87 11 to to TO 10_1101-2021_02_08_430275 87 12 be be VB 10_1101-2021_02_08_430275 87 13 very very RB 10_1101-2021_02_08_430275 87 14 lenient lenient JJ 10_1101-2021_02_08_430275 87 15 in in IN 10_1101-2021_02_08_430275 87 16 order order NN 10_1101-2021_02_08_430275 87 17 to to TO 10_1101-2021_02_08_430275 87 18 achieve achieve VB 10_1101-2021_02_08_430275 87 19 a a DT 10_1101-2021_02_08_430275 87 20 high high JJ 10_1101-2021_02_08_430275 87 21 degree223 degree223 NN 10_1101-2021_02_08_430275 87 22 of of IN 10_1101-2021_02_08_430275 87 23 sensitivity sensitivity NN 10_1101-2021_02_08_430275 87 24 ( ( -LRB- 10_1101-2021_02_08_430275 87 25 https://gatk.broadinstitute.org/hc/en-us/articles/224 https://gatk.broadinstitute.org/hc/en-us/articles/224 CD 10_1101-2021_02_08_430275 87 26 360035535932-Germline 360035535932-germline CD 10_1101-2021_02_08_430275 87 27 - - HYPH 10_1101-2021_02_08_430275 87 28 short short JJ 10_1101-2021_02_08_430275 87 29 - - HYPH 10_1101-2021_02_08_430275 87 30 variant variant JJ 10_1101-2021_02_08_430275 87 31 - - HYPH 10_1101-2021_02_08_430275 87 32 discovery discovery NN 10_1101-2021_02_08_430275 87 33 - - HYPH 10_1101-2021_02_08_430275 87 34 SNPs snps NN 10_1101-2021_02_08_430275 87 35 - - HYPH 10_1101-2021_02_08_430275 87 36 Indels-),225 indels-),225 NN 10_1101-2021_02_08_430275 87 37 we -PRON- PRP 10_1101-2021_02_08_430275 87 38 can can MD 10_1101-2021_02_08_430275 87 39 not not RB 10_1101-2021_02_08_430275 87 40 rule rule VB 10_1101-2021_02_08_430275 87 41 out out RP 10_1101-2021_02_08_430275 87 42 the the DT 10_1101-2021_02_08_430275 87 43 possibility possibility NN 10_1101-2021_02_08_430275 87 44 that that IN 10_1101-2021_02_08_430275 87 45 some some DT 10_1101-2021_02_08_430275 87 46 of of IN 10_1101-2021_02_08_430275 87 47 the the DT 10_1101-2021_02_08_430275 87 48 heterozy-226 heterozy-226 NNP 10_1101-2021_02_08_430275 87 49 gous gous JJ 10_1101-2021_02_08_430275 87 50 loci loci NN 10_1101-2021_02_08_430275 87 51 were be VBD 10_1101-2021_02_08_430275 87 52 caused cause VBN 10_1101-2021_02_08_430275 87 53 by by IN 10_1101-2021_02_08_430275 87 54 sequencing sequence VBG 10_1101-2021_02_08_430275 87 55 artifacts artifact NNS 10_1101-2021_02_08_430275 87 56 . . . 10_1101-2021_02_08_430275 88 1 The the DT 10_1101-2021_02_08_430275 88 2 bulk bulk JJ 10_1101-2021_02_08_430275 88 3 SNP227 SNP227 NNP 10_1101-2021_02_08_430275 88 4 dataset dataset NN 10_1101-2021_02_08_430275 88 5 was be VBD 10_1101-2021_02_08_430275 88 6 processed process VBN 10_1101-2021_02_08_430275 88 7 second second JJ 10_1101-2021_02_08_430275 88 8 . . . 10_1101-2021_02_08_430275 89 1 The the DT 10_1101-2021_02_08_430275 89 2 SNPs snp NNS 10_1101-2021_02_08_430275 89 3 with with IN 10_1101-2021_02_08_430275 89 4 the the DT 10_1101-2021_02_08_430275 89 5 same228 same228 JJ 10_1101-2021_02_08_430275 89 6 chromosome chromosome NN 10_1101-2021_02_08_430275 89 7 ID ID NNP 10_1101-2021_02_08_430275 89 8 and and CC 10_1101-2021_02_08_430275 89 9 the the DT 10_1101-2021_02_08_430275 89 10 same same JJ 10_1101-2021_02_08_430275 89 11 genomic genomic JJ 10_1101-2021_02_08_430275 89 12 coordinate coordinate NN 10_1101-2021_02_08_430275 89 13 in in IN 10_1101-2021_02_08_430275 89 14 both229 both229 NNP 10_1101-2021_02_08_430275 89 15 datasets dataset NNS 10_1101-2021_02_08_430275 89 16 were be VBD 10_1101-2021_02_08_430275 89 17 considered consider VBN 10_1101-2021_02_08_430275 89 18 common common JJ 10_1101-2021_02_08_430275 89 19 SNPs snp NNS 10_1101-2021_02_08_430275 89 20 . . . 10_1101-2021_02_08_430275 90 1 Common common JJ 10_1101-2021_02_08_430275 90 2 SNPs snp NNS 10_1101-2021_02_08_430275 90 3 in230 in230 VBP 10_1101-2021_02_08_430275 90 4 the the DT 10_1101-2021_02_08_430275 90 5 bulk bulk JJ 10_1101-2021_02_08_430275 90 6 dataset dataset NN 10_1101-2021_02_08_430275 90 7 were be VBD 10_1101-2021_02_08_430275 90 8 used use VBN 10_1101-2021_02_08_430275 90 9 to to TO 10_1101-2021_02_08_430275 90 10 detect detect VB 10_1101-2021_02_08_430275 90 11 SNP SNP NNP 10_1101-2021_02_08_430275 90 12 - - HYPH 10_1101-2021_02_08_430275 90 13 trait trait NN 10_1101-2021_02_08_430275 90 14 associations231 associations231 NNP 10_1101-2021_02_08_430275 90 15 for for IN 10_1101-2021_02_08_430275 90 16 all all DT 10_1101-2021_02_08_430275 90 17 three three CD 10_1101-2021_02_08_430275 90 18 methods.232 methods.232 NNP 10_1101-2021_02_08_430275 90 19 Table table NN 10_1101-2021_02_08_430275 90 20 1 1 CD 10_1101-2021_02_08_430275 90 21 . . . 10_1101-2021_02_08_430275 91 1 Chromosomal chromosomal JJ 10_1101-2021_02_08_430275 91 2 distribution distribution NN 10_1101-2021_02_08_430275 91 3 of of IN 10_1101-2021_02_08_430275 91 4 SNPs snp NNS 10_1101-2021_02_08_430275 91 5 - - , 10_1101-2021_02_08_430275 91 6 using use VBG 10_1101-2021_02_08_430275 91 7 the the DT 10_1101-2021_02_08_430275 91 8 genome genome JJ 10_1101-2021_02_08_430275 91 9 sequences sequence NNS 10_1101-2021_02_08_430275 91 10 of of IN 10_1101-2021_02_08_430275 91 11 both both PDT 10_1101-2021_02_08_430275 91 12 the the DT 10_1101-2021_02_08_430275 91 13 parents parent NNS 10_1101-2021_02_08_430275 91 14 and and CC 10_1101-2021_02_08_430275 91 15 the the DT 10_1101-2021_02_08_430275 91 16 bulks bulk NNS 10_1101-2021_02_08_430275 91 17 Chromosome Chromosome NNP 10_1101-2021_02_08_430275 91 18 sSNPs ssnps CD 10_1101-2021_02_08_430275 91 19 TotalSNPs totalsnps RB 10_1101-2021_02_08_430275 91 20 sSNP ssnp NN 10_1101-2021_02_08_430275 91 21 / / SYM 10_1101-2021_02_08_430275 91 22 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 91 23 1 1 CD 10_1101-2021_02_08_430275 91 24 1170 1170 CD 10_1101-2021_02_08_430275 91 25 139 139 CD 10_1101-2021_02_08_430275 91 26 910 910 CD 10_1101-2021_02_08_430275 91 27 0.0084 0.0084 CD 10_1101-2021_02_08_430275 91 28 2 2 CD 10_1101-2021_02_08_430275 91 29 310 310 CD 10_1101-2021_02_08_430275 91 30 125 125 CD 10_1101-2021_02_08_430275 91 31 129 129 CD 10_1101-2021_02_08_430275 91 32 0.0025 0.0025 CD 10_1101-2021_02_08_430275 91 33 3 3 CD 10_1101-2021_02_08_430275 91 34 459 459 CD 10_1101-2021_02_08_430275 91 35 102 102 CD 10_1101-2021_02_08_430275 91 36 331 331 CD 10_1101-2021_02_08_430275 91 37 0.0045 0.0045 CD 10_1101-2021_02_08_430275 91 38 4 4 CD 10_1101-2021_02_08_430275 91 39 330 330 CD 10_1101-2021_02_08_430275 91 40 89 89 CD 10_1101-2021_02_08_430275 91 41 577 577 CD 10_1101-2021_02_08_430275 91 42 0.0037 0.0037 CD 10_1101-2021_02_08_430275 91 43 5 5 CD 10_1101-2021_02_08_430275 91 44 372 372 CD 10_1101-2021_02_08_430275 91 45 84 84 CD 10_1101-2021_02_08_430275 91 46 706 706 CD 10_1101-2021_02_08_430275 91 47 0.0044 0.0044 CD 10_1101-2021_02_08_430275 91 48 6 6 CD 10_1101-2021_02_08_430275 91 49 1581 1581 CD 10_1101-2021_02_08_430275 91 50 83 83 CD 10_1101-2021_02_08_430275 91 51 605 605 CD 10_1101-2021_02_08_430275 91 52 0.0189 0.0189 CD 10_1101-2021_02_08_430275 91 53 7 7 CD 10_1101-2021_02_08_430275 91 54 378 378 CD 10_1101-2021_02_08_430275 91 55 94 94 CD 10_1101-2021_02_08_430275 91 56 371 371 CD 10_1101-2021_02_08_430275 91 57 0.0040 0.0040 CD 10_1101-2021_02_08_430275 91 58 8 8 CD 10_1101-2021_02_08_430275 91 59 258 258 CD 10_1101-2021_02_08_430275 91 60 80 80 CD 10_1101-2021_02_08_430275 91 61 617 617 CD 10_1101-2021_02_08_430275 91 62 0.0032 0.0032 CD 10_1101-2021_02_08_430275 91 63 9 9 CD 10_1101-2021_02_08_430275 91 64 1292 1292 CD 10_1101-2021_02_08_430275 91 65 67 67 CD 10_1101-2021_02_08_430275 91 66 157 157 CD 10_1101-2021_02_08_430275 91 67 0.0192 0.0192 CD 10_1101-2021_02_08_430275 91 68 10 10 CD 10_1101-2021_02_08_430275 91 69 363 363 CD 10_1101-2021_02_08_430275 91 70 56 56 CD 10_1101-2021_02_08_430275 91 71 681 681 CD 10_1101-2021_02_08_430275 91 72 0.0064 0.0064 CD 10_1101-2021_02_08_430275 91 73 11 11 CD 10_1101-2021_02_08_430275 91 74 2765 2765 CD 10_1101-2021_02_08_430275 91 75 88 88 CD 10_1101-2021_02_08_430275 91 76 287 287 CD 10_1101-2021_02_08_430275 91 77 0.0313 0.0313 CD 10_1101-2021_02_08_430275 91 78 12 12 CD 10_1101-2021_02_08_430275 91 79 241 241 CD 10_1101-2021_02_08_430275 91 80 87 87 CD 10_1101-2021_02_08_430275 91 81 145 145 CD 10_1101-2021_02_08_430275 91 82 0.0028 0.0028 CD 10_1101-2021_02_08_430275 91 83 Genome genome NN 10_1101-2021_02_08_430275 91 84 - - HYPH 10_1101-2021_02_08_430275 91 85 wide wide JJ 10_1101-2021_02_08_430275 91 86 9519 9519 CD 10_1101-2021_02_08_430275 91 87 1 1 CD 10_1101-2021_02_08_430275 91 88 099 099 CD 10_1101-2021_02_08_430275 91 89 516 516 CD 10_1101-2021_02_08_430275 91 90 0.0087 0.0087 CD 10_1101-2021_02_08_430275 91 91 The the DT 10_1101-2021_02_08_430275 91 92 significant significant JJ 10_1101-2021_02_08_430275 91 93 structural structural JJ 10_1101-2021_02_08_430275 91 94 variant variant JJ 10_1101-2021_02_08_430275 91 95 method method NN 10_1101-2021_02_08_430275 91 96 : : : 10_1101-2021_02_08_430275 91 97 Each each DT 10_1101-2021_02_08_430275 91 98 SNP SNP NNP 10_1101-2021_02_08_430275 91 99 in in IN 10_1101-2021_02_08_430275 91 100 the233 the233 DT 10_1101-2021_02_08_430275 91 101 dataset dataset NN 10_1101-2021_02_08_430275 91 102 was be VBD 10_1101-2021_02_08_430275 91 103 tested test VBN 10_1101-2021_02_08_430275 91 104 via via IN 10_1101-2021_02_08_430275 91 105 Fisher Fisher NNP 10_1101-2021_02_08_430275 91 106 ’s ’s POS 10_1101-2021_02_08_430275 91 107 exact exact JJ 10_1101-2021_02_08_430275 91 108 test test NN 10_1101-2021_02_08_430275 91 109 using use VBG 10_1101-2021_02_08_430275 91 110 its -PRON- PRP$ 10_1101-2021_02_08_430275 91 111 four four CD 10_1101-2021_02_08_430275 91 112 AD234 AD234 NNP 10_1101-2021_02_08_430275 91 113 values value NNS 10_1101-2021_02_08_430275 91 114 , , , 10_1101-2021_02_08_430275 91 115 and and CC 10_1101-2021_02_08_430275 91 116 SNPs SNPs NNPS 10_1101-2021_02_08_430275 91 117 with with IN 10_1101-2021_02_08_430275 91 118 P p NN 10_1101-2021_02_08_430275 91 119 - - HYPH 10_1101-2021_02_08_430275 91 120 values value NNS 10_1101-2021_02_08_430275 91 121 less less JJR 10_1101-2021_02_08_430275 91 122 than than IN 10_1101-2021_02_08_430275 91 123 0.01 0.01 CD 10_1101-2021_02_08_430275 91 124 were be VBD 10_1101-2021_02_08_430275 91 125 defined235 defined235 NNP 10_1101-2021_02_08_430275 91 126 as as IN 10_1101-2021_02_08_430275 91 127 sSNPs ssnps CD 10_1101-2021_02_08_430275 91 128 . . . 10_1101-2021_02_08_430275 92 1 The the DT 10_1101-2021_02_08_430275 92 2 chromosomal chromosomal JJ 10_1101-2021_02_08_430275 92 3 distributions distribution NNS 10_1101-2021_02_08_430275 92 4 of of IN 10_1101-2021_02_08_430275 92 5 the the DT 10_1101-2021_02_08_430275 92 6 sSNPs ssnps CD 10_1101-2021_02_08_430275 92 7 and236 and236 SYM 10_1101-2021_02_08_430275 92 8 the the DT 10_1101-2021_02_08_430275 92 9 total total JJ 10_1101-2021_02_08_430275 92 10 SNPs snp NNS 10_1101-2021_02_08_430275 92 11 are be VBP 10_1101-2021_02_08_430275 92 12 summarized summarize VBN 10_1101-2021_02_08_430275 92 13 in in IN 10_1101-2021_02_08_430275 92 14 Table table NN 10_1101-2021_02_08_430275 92 15 1 1 CD 10_1101-2021_02_08_430275 92 16 . . . 10_1101-2021_02_08_430275 93 1 Using use VBG 10_1101-2021_02_08_430275 93 2 the the DT 10_1101-2021_02_08_430275 93 3 sliding237 sliding237 NNP 10_1101-2021_02_08_430275 93 4 window window NN 10_1101-2021_02_08_430275 93 5 algorithm algorithm NN 10_1101-2021_02_08_430275 93 6 , , , 10_1101-2021_02_08_430275 93 7 the the DT 10_1101-2021_02_08_430275 93 8 genomic genomic JJ 10_1101-2021_02_08_430275 93 9 distribution distribution NN 10_1101-2021_02_08_430275 93 10 of of IN 10_1101-2021_02_08_430275 93 11 the the DT 10_1101-2021_02_08_430275 93 12 sSNPs ssnps CD 10_1101-2021_02_08_430275 93 13 , , , 10_1101-2021_02_08_430275 93 14 the238 the238 DT 10_1101-2021_02_08_430275 93 15 total total JJ 10_1101-2021_02_08_430275 93 16 SNPs snp NNS 10_1101-2021_02_08_430275 93 17 , , , 10_1101-2021_02_08_430275 93 18 and and CC 10_1101-2021_02_08_430275 93 19 the the DT 10_1101-2021_02_08_430275 93 20 sSNP ssnp NN 10_1101-2021_02_08_430275 93 21 / / SYM 10_1101-2021_02_08_430275 93 22 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 93 23 ratios ratio NNS 10_1101-2021_02_08_430275 93 24 of of IN 10_1101-2021_02_08_430275 93 25 sliding slide VBG 10_1101-2021_02_08_430275 93 26 windows239 windows239 NNP 10_1101-2021_02_08_430275 93 27 were be VBD 10_1101-2021_02_08_430275 93 28 plotted plot VBN 10_1101-2021_02_08_430275 93 29 against against IN 10_1101-2021_02_08_430275 93 30 their -PRON- PRP$ 10_1101-2021_02_08_430275 93 31 genomic genomic JJ 10_1101-2021_02_08_430275 93 32 position position NN 10_1101-2021_02_08_430275 93 33 ( ( -LRB- 10_1101-2021_02_08_430275 93 34 Figure figure NN 10_1101-2021_02_08_430275 93 35 1a 1a NNP 10_1101-2021_02_08_430275 93 36 and240 and240 NNP 10_1101-2021_02_08_430275 93 37 Figure Figure NNP 10_1101-2021_02_08_430275 93 38 1b 1b NN 10_1101-2021_02_08_430275 93 39 ) ) -RRB- 10_1101-2021_02_08_430275 93 40 . . . 10_1101-2021_02_08_430275 94 1 A a DT 10_1101-2021_02_08_430275 94 2 genome genome JJ 10_1101-2021_02_08_430275 94 3 - - HYPH 10_1101-2021_02_08_430275 94 4 wide wide JJ 10_1101-2021_02_08_430275 94 5 threshold threshold NN 10_1101-2021_02_08_430275 94 6 was be VBD 10_1101-2021_02_08_430275 94 7 estimated estimate VBN 10_1101-2021_02_08_430275 94 8 as as IN 10_1101-2021_02_08_430275 94 9 0.0538241 0.0538241 CD 10_1101-2021_02_08_430275 94 10 via via IN 10_1101-2021_02_08_430275 94 11 simulation simulation NN 10_1101-2021_02_08_430275 94 12 as as IN 10_1101-2021_02_08_430275 94 13 described describe VBN 10_1101-2021_02_08_430275 94 14 previously previously RB 10_1101-2021_02_08_430275 94 15 ( ( -LRB- 10_1101-2021_02_08_430275 94 16 8) 8) CD 10_1101-2021_02_08_430275 94 17 . . . 10_1101-2021_02_08_430275 95 1 Two two CD 10_1101-2021_02_08_430275 95 2 peaks peak VBZ 10_1101-2021_02_08_430275 95 3 above242 above242 PRP 10_1101-2021_02_08_430275 95 4 the the DT 10_1101-2021_02_08_430275 95 5 threshold threshold NN 10_1101-2021_02_08_430275 95 6 were be VBD 10_1101-2021_02_08_430275 95 7 identified identify VBN 10_1101-2021_02_08_430275 95 8 : : : 10_1101-2021_02_08_430275 95 9 a a DT 10_1101-2021_02_08_430275 95 10 minor minor JJ 10_1101-2021_02_08_430275 95 11 one one NN 10_1101-2021_02_08_430275 95 12 on on IN 10_1101-2021_02_08_430275 95 13 chromosome243 chromosome243 NNP 10_1101-2021_02_08_430275 95 14 9 9 CD 10_1101-2021_02_08_430275 95 15 and and CC 10_1101-2021_02_08_430275 95 16 a a DT 10_1101-2021_02_08_430275 95 17 major major JJ 10_1101-2021_02_08_430275 95 18 one one CD 10_1101-2021_02_08_430275 95 19 on on IN 10_1101-2021_02_08_430275 95 20 chromosome chromosome NN 10_1101-2021_02_08_430275 95 21 11 11 CD 10_1101-2021_02_08_430275 95 22 . . . 10_1101-2021_02_08_430275 96 1 The the DT 10_1101-2021_02_08_430275 96 2 position position NN 10_1101-2021_02_08_430275 96 3 of of IN 10_1101-2021_02_08_430275 96 4 the244 the244 NNP 10_1101-2021_02_08_430275 96 5 peak peak NN 10_1101-2021_02_08_430275 96 6 on on IN 10_1101-2021_02_08_430275 96 7 chromosome chromosome NN 10_1101-2021_02_08_430275 96 8 9 9 CD 10_1101-2021_02_08_430275 96 9 was be VBD 10_1101-2021_02_08_430275 96 10 at at IN 10_1101-2021_02_08_430275 96 11 1.11 1.11 CD 10_1101-2021_02_08_430275 96 12 Mb Mb NNS 10_1101-2021_02_08_430275 96 13 , , , 10_1101-2021_02_08_430275 96 14 the the DT 10_1101-2021_02_08_430275 96 15 sliding slide VBG 10_1101-2021_02_08_430275 96 16 window245 window245 NNP 10_1101-2021_02_08_430275 96 17 contained contain VBD 10_1101-2021_02_08_430275 96 18 230 230 CD 10_1101-2021_02_08_430275 96 19 sSNPs ssnps CD 10_1101-2021_02_08_430275 96 20 and and CC 10_1101-2021_02_08_430275 96 21 3738 3738 CD 10_1101-2021_02_08_430275 96 22 total total JJ 10_1101-2021_02_08_430275 96 23 SNPs snp NNS 10_1101-2021_02_08_430275 96 24 , , , 10_1101-2021_02_08_430275 96 25 corresponding246 corresponding246 VBD 10_1101-2021_02_08_430275 96 26 to to IN 10_1101-2021_02_08_430275 96 27 an an DT 10_1101-2021_02_08_430275 96 28 sSNP ssnp NN 10_1101-2021_02_08_430275 96 29 / / SYM 10_1101-2021_02_08_430275 96 30 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 96 31 ratio ratio NN 10_1101-2021_02_08_430275 96 32 of of IN 10_1101-2021_02_08_430275 96 33 0.0615 0.0615 CD 10_1101-2021_02_08_430275 96 34 ; ; : 10_1101-2021_02_08_430275 96 35 the the DT 10_1101-2021_02_08_430275 96 36 position position NN 10_1101-2021_02_08_430275 96 37 of of IN 10_1101-2021_02_08_430275 96 38 the247 the247 CD 10_1101-2021_02_08_430275 96 39 peak peak VB 10_1101-2021_02_08_430275 96 40 on on IN 10_1101-2021_02_08_430275 96 41 chromosome chromosome NN 10_1101-2021_02_08_430275 96 42 11 11 CD 10_1101-2021_02_08_430275 96 43 was be VBD 10_1101-2021_02_08_430275 96 44 at at IN 10_1101-2021_02_08_430275 96 45 26.44 26.44 CD 10_1101-2021_02_08_430275 96 46 Mb Mb NNP 10_1101-2021_02_08_430275 96 47 , , , 10_1101-2021_02_08_430275 96 48 the the DT 10_1101-2021_02_08_430275 96 49 sliding slide VBG 10_1101-2021_02_08_430275 96 50 window248 window248 NNP 10_1101-2021_02_08_430275 96 51 contained contain VBD 10_1101-2021_02_08_430275 96 52 675 675 CD 10_1101-2021_02_08_430275 96 53 sSNPs ssnps CD 10_1101-2021_02_08_430275 96 54 and and CC 10_1101-2021_02_08_430275 96 55 1139 1139 CD 10_1101-2021_02_08_430275 96 56 total total JJ 10_1101-2021_02_08_430275 96 57 SNPs snp NNS 10_1101-2021_02_08_430275 96 58 , , , 10_1101-2021_02_08_430275 96 59 corresponding correspond VBG 10_1101-2021_02_08_430275 96 60 to to IN 10_1101-2021_02_08_430275 96 61 an249 an249 NNP 10_1101-2021_02_08_430275 96 62 sSNP ssnp NN 10_1101-2021_02_08_430275 96 63 / / SYM 10_1101-2021_02_08_430275 96 64 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 96 65 ratio ratio NN 10_1101-2021_02_08_430275 96 66 of of IN 10_1101-2021_02_08_430275 96 67 0.5926 0.5926 CD 10_1101-2021_02_08_430275 96 68 . . . 10_1101-2021_02_08_430275 97 1 The the DT 10_1101-2021_02_08_430275 97 2 sliding slide VBG 10_1101-2021_02_08_430275 97 3 window window NN 10_1101-2021_02_08_430275 97 4 - - HYPH 10_1101-2021_02_08_430275 97 5 specific specific JJ 10_1101-2021_02_08_430275 97 6 250 250 CD 10_1101-2021_02_08_430275 97 7 threshold threshold NN 10_1101-2021_02_08_430275 97 8 was be VBD 10_1101-2021_02_08_430275 97 9 estimated estimate VBN 10_1101-2021_02_08_430275 97 10 for for IN 10_1101-2021_02_08_430275 97 11 each each DT 10_1101-2021_02_08_430275 97 12 peak peak NN 10_1101-2021_02_08_430275 97 13 via via IN 10_1101-2021_02_08_430275 97 14 simulation simulation NN 10_1101-2021_02_08_430275 97 15 , , , 10_1101-2021_02_08_430275 97 16 and and CC 10_1101-2021_02_08_430275 97 17 251 251 CD 10_1101-2021_02_08_430275 97 18 the the DT 10_1101-2021_02_08_430275 97 19 values value NNS 10_1101-2021_02_08_430275 97 20 were be VBD 10_1101-2021_02_08_430275 97 21 0.0551 0.0551 CD 10_1101-2021_02_08_430275 97 22 and and CC 10_1101-2021_02_08_430275 97 23 0.0623 0.0623 CD 10_1101-2021_02_08_430275 97 24 , , , 10_1101-2021_02_08_430275 97 25 respectively respectively RB 10_1101-2021_02_08_430275 97 26 , , , 10_1101-2021_02_08_430275 97 27 indicating indicate VBG 10_1101-2021_02_08_430275 97 28 252 252 CD 10_1101-2021_02_08_430275 97 29 both both DT 10_1101-2021_02_08_430275 97 30 peaks peak NNS 10_1101-2021_02_08_430275 97 31 were be VBD 10_1101-2021_02_08_430275 97 32 significant significant JJ 10_1101-2021_02_08_430275 97 33 . . . 10_1101-2021_02_08_430275 98 1 Both both DT 10_1101-2021_02_08_430275 98 2 values value NNS 10_1101-2021_02_08_430275 98 3 are be VBP 10_1101-2021_02_08_430275 98 4 higher high JJR 10_1101-2021_02_08_430275 98 5 than than IN 10_1101-2021_02_08_430275 98 6 the the DT 10_1101-2021_02_08_430275 98 7 253 253 CD 10_1101-2021_02_08_430275 98 8 genome genome NN 10_1101-2021_02_08_430275 98 9 - - HYPH 10_1101-2021_02_08_430275 98 10 wide wide JJ 10_1101-2021_02_08_430275 98 11 threshold threshold NN 10_1101-2021_02_08_430275 98 12 , , , 10_1101-2021_02_08_430275 98 13 probably probably RB 10_1101-2021_02_08_430275 98 14 due due IN 10_1101-2021_02_08_430275 98 15 to to IN 10_1101-2021_02_08_430275 98 16 the the DT 10_1101-2021_02_08_430275 98 17 lower low JJR 10_1101-2021_02_08_430275 98 18 amounts amount NNS 10_1101-2021_02_08_430275 98 19 of of IN 10_1101-2021_02_08_430275 98 20 254 254 CD 10_1101-2021_02_08_430275 98 21 total total JJ 10_1101-2021_02_08_430275 98 22 SNPs snp NNS 10_1101-2021_02_08_430275 98 23 in in IN 10_1101-2021_02_08_430275 98 24 these these DT 10_1101-2021_02_08_430275 98 25 sliding slide VBG 10_1101-2021_02_08_430275 98 26 windows window NNS 10_1101-2021_02_08_430275 98 27 . . . 10_1101-2021_02_08_430275 99 1 The the DT 10_1101-2021_02_08_430275 99 2 average average JJ 10_1101-2021_02_08_430275 99 3 SNPs snp NNS 10_1101-2021_02_08_430275 99 4 per per IN 10_1101-2021_02_08_430275 99 5 255 255 CD 10_1101-2021_02_08_430275 99 6 sliding slide VBG 10_1101-2021_02_08_430275 99 7 window window NN 10_1101-2021_02_08_430275 99 8 was be VBD 10_1101-2021_02_08_430275 99 9 5893 5893 CD 10_1101-2021_02_08_430275 99 10 . . . 10_1101-2021_02_08_430275 100 1 256 256 CD 10_1101-2021_02_08_430275 100 2 The the DT 10_1101-2021_02_08_430275 100 3 G g NN 10_1101-2021_02_08_430275 100 4 - - HYPH 10_1101-2021_02_08_430275 100 5 statistic statistic NN 10_1101-2021_02_08_430275 100 6 method method NN 10_1101-2021_02_08_430275 100 7 : : : 10_1101-2021_02_08_430275 100 8 The the DT 10_1101-2021_02_08_430275 100 9 G g NN 10_1101-2021_02_08_430275 100 10 - - HYPH 10_1101-2021_02_08_430275 100 11 statistic statistic NN 10_1101-2021_02_08_430275 100 12 value value NN 10_1101-2021_02_08_430275 100 13 of of IN 10_1101-2021_02_08_430275 100 14 each each DT 10_1101-2021_02_08_430275 100 15 SNP SNP NNP 10_1101-2021_02_08_430275 100 16 257 257 CD 10_1101-2021_02_08_430275 100 17 in in IN 10_1101-2021_02_08_430275 100 18 the the DT 10_1101-2021_02_08_430275 100 19 dataset dataset NN 10_1101-2021_02_08_430275 100 20 was be VBD 10_1101-2021_02_08_430275 100 21 calculated calculate VBN 10_1101-2021_02_08_430275 100 22 , , , 10_1101-2021_02_08_430275 100 23 and and CC 10_1101-2021_02_08_430275 100 24 its -PRON- PRP$ 10_1101-2021_02_08_430275 100 25 threshold threshold NN 10_1101-2021_02_08_430275 100 26 was be VBD 10_1101-2021_02_08_430275 100 27 estimated estimate VBN 10_1101-2021_02_08_430275 100 28 258 258 CD 10_1101-2021_02_08_430275 100 29 via via IN 10_1101-2021_02_08_430275 100 30 simulation simulation NN 10_1101-2021_02_08_430275 100 31 as as IN 10_1101-2021_02_08_430275 100 32 described describe VBN 10_1101-2021_02_08_430275 100 33 previously previously RB 10_1101-2021_02_08_430275 100 34 ( ( -LRB- 10_1101-2021_02_08_430275 100 35 8) 8) CD 10_1101-2021_02_08_430275 100 36 . . . 10_1101-2021_02_08_430275 101 1 Using use VBG 10_1101-2021_02_08_430275 101 2 the the DT 10_1101-2021_02_08_430275 101 3 sliding slide VBG 10_1101-2021_02_08_430275 101 4 259 259 CD 10_1101-2021_02_08_430275 101 5 window window NN 10_1101-2021_02_08_430275 101 6 algorithm algorithm NN 10_1101-2021_02_08_430275 101 7 , , , 10_1101-2021_02_08_430275 101 8 the the DT 10_1101-2021_02_08_430275 101 9 G g NN 10_1101-2021_02_08_430275 101 10 - - HYPH 10_1101-2021_02_08_430275 101 11 statistic statistic NN 10_1101-2021_02_08_430275 101 12 value value NN 10_1101-2021_02_08_430275 101 13 of of IN 10_1101-2021_02_08_430275 101 14 each each DT 10_1101-2021_02_08_430275 101 15 sliding slide VBG 10_1101-2021_02_08_430275 101 16 win- win- XX 10_1101-2021_02_08_430275 101 17 260 260 CD 10_1101-2021_02_08_430275 101 18 dow dow NNP 10_1101-2021_02_08_430275 101 19 , , , 10_1101-2021_02_08_430275 101 20 the the DT 10_1101-2021_02_08_430275 101 21 average average JJ 10_1101-2021_02_08_430275 101 22 G g NN 10_1101-2021_02_08_430275 101 23 - - HYPH 10_1101-2021_02_08_430275 101 24 statistic statistic NN 10_1101-2021_02_08_430275 101 25 values value NNS 10_1101-2021_02_08_430275 101 26 of of IN 10_1101-2021_02_08_430275 101 27 all all DT 10_1101-2021_02_08_430275 101 28 SNPs snp NNS 10_1101-2021_02_08_430275 101 29 in in IN 10_1101-2021_02_08_430275 101 30 that that DT 10_1101-2021_02_08_430275 101 31 sliding slide VBG 10_1101-2021_02_08_430275 101 32 261 261 CD 10_1101-2021_02_08_430275 101 33 window window NN 10_1101-2021_02_08_430275 101 34 , , , 10_1101-2021_02_08_430275 101 35 was be VBD 10_1101-2021_02_08_430275 101 36 plotted plot VBN 10_1101-2021_02_08_430275 101 37 against against IN 10_1101-2021_02_08_430275 101 38 its -PRON- PRP$ 10_1101-2021_02_08_430275 101 39 genomic genomic JJ 10_1101-2021_02_08_430275 101 40 position position NN 10_1101-2021_02_08_430275 101 41 ( ( -LRB- 10_1101-2021_02_08_430275 101 42 Figure Figure NNP 10_1101-2021_02_08_430275 101 43 1c 1c NNP 10_1101-2021_02_08_430275 101 44 ) ) -RRB- 10_1101-2021_02_08_430275 101 45 , , , 10_1101-2021_02_08_430275 101 46 262 262 CD 10_1101-2021_02_08_430275 101 47 and and CC 10_1101-2021_02_08_430275 101 48 the the DT 10_1101-2021_02_08_430275 101 49 curve curve NN 10_1101-2021_02_08_430275 101 50 pattern pattern NN 10_1101-2021_02_08_430275 101 51 was be VBD 10_1101-2021_02_08_430275 101 52 very very RB 10_1101-2021_02_08_430275 101 53 similar similar JJ 10_1101-2021_02_08_430275 101 54 to to IN 10_1101-2021_02_08_430275 101 55 that that DT 10_1101-2021_02_08_430275 101 56 in in IN 10_1101-2021_02_08_430275 101 57 Figure Figure NNP 10_1101-2021_02_08_430275 101 58 1b 1b NN 10_1101-2021_02_08_430275 101 59 . . . 10_1101-2021_02_08_430275 102 1 A a DT 10_1101-2021_02_08_430275 102 2 263 263 CD 10_1101-2021_02_08_430275 102 3 significant significant JJ 10_1101-2021_02_08_430275 102 4 peak peak NN 10_1101-2021_02_08_430275 102 5 was be VBD 10_1101-2021_02_08_430275 102 6 identified identify VBN 10_1101-2021_02_08_430275 102 7 on on IN 10_1101-2021_02_08_430275 102 8 chromosome chromosome NN 10_1101-2021_02_08_430275 102 9 11 11 CD 10_1101-2021_02_08_430275 102 10 ; ; : 10_1101-2021_02_08_430275 102 11 its -PRON- PRP$ 10_1101-2021_02_08_430275 102 12 position position NN 10_1101-2021_02_08_430275 102 13 264 264 CD 10_1101-2021_02_08_430275 102 14 was be VBD 10_1101-2021_02_08_430275 102 15 at at IN 10_1101-2021_02_08_430275 102 16 26.49 26.49 CD 10_1101-2021_02_08_430275 102 17 Mb Mb NNS 10_1101-2021_02_08_430275 102 18 , , , 10_1101-2021_02_08_430275 102 19 its -PRON- PRP$ 10_1101-2021_02_08_430275 102 20 G g NN 10_1101-2021_02_08_430275 102 21 - - HYPH 10_1101-2021_02_08_430275 102 22 statistic statistic NN 10_1101-2021_02_08_430275 102 23 value value NN 10_1101-2021_02_08_430275 102 24 was be VBD 10_1101-2021_02_08_430275 102 25 12.8120 12.8120 CD 10_1101-2021_02_08_430275 102 26 , , , 10_1101-2021_02_08_430275 102 27 well well RB 10_1101-2021_02_08_430275 102 28 above above IN 10_1101-2021_02_08_430275 102 29 265 265 CD 10_1101-2021_02_08_430275 102 30 the the DT 10_1101-2021_02_08_430275 102 31 threshold threshold NN 10_1101-2021_02_08_430275 102 32 9.0224 9.0224 CD 10_1101-2021_02_08_430275 102 33 ( ( -LRB- 10_1101-2021_02_08_430275 102 34 99.5th 99.5th NN 10_1101-2021_02_08_430275 102 35 percentile percentile NN 10_1101-2021_02_08_430275 102 36 ) ) -RRB- 10_1101-2021_02_08_430275 102 37 . . . 10_1101-2021_02_08_430275 103 1 266 266 CD 10_1101-2021_02_08_430275 103 2 The the DT 10_1101-2021_02_08_430275 103 3 allele allele NN 10_1101-2021_02_08_430275 103 4 frequency frequency NN 10_1101-2021_02_08_430275 103 5 method method NN 10_1101-2021_02_08_430275 103 6 : : : 10_1101-2021_02_08_430275 103 7 The the DT 10_1101-2021_02_08_430275 103 8 ΔAF δaf NN 10_1101-2021_02_08_430275 103 9 value value NN 10_1101-2021_02_08_430275 103 10 of of IN 10_1101-2021_02_08_430275 103 11 each each DT 10_1101-2021_02_08_430275 103 12 SNP SNP NNP 10_1101-2021_02_08_430275 103 13 in in IN 10_1101-2021_02_08_430275 103 14 267 267 CD 10_1101-2021_02_08_430275 103 15 the the DT 10_1101-2021_02_08_430275 103 16 dataset dataset NN 10_1101-2021_02_08_430275 103 17 was be VBD 10_1101-2021_02_08_430275 103 18 calculated calculate VBN 10_1101-2021_02_08_430275 103 19 , , , 10_1101-2021_02_08_430275 103 20 and and CC 10_1101-2021_02_08_430275 103 21 the the DT 10_1101-2021_02_08_430275 103 22 ΔAF δaf NN 10_1101-2021_02_08_430275 103 23 threshold threshold NN 10_1101-2021_02_08_430275 103 24 of of IN 10_1101-2021_02_08_430275 103 25 the the DT 10_1101-2021_02_08_430275 103 26 SNP SNP NNP 10_1101-2021_02_08_430275 103 27 268 268 CD 10_1101-2021_02_08_430275 103 28 was be VBD 10_1101-2021_02_08_430275 103 29 estimated estimate VBN 10_1101-2021_02_08_430275 103 30 via via IN 10_1101-2021_02_08_430275 103 31 simulation simulation NN 10_1101-2021_02_08_430275 103 32 as as IN 10_1101-2021_02_08_430275 103 33 described describe VBN 10_1101-2021_02_08_430275 103 34 previously previously RB 10_1101-2021_02_08_430275 103 35 ( ( -LRB- 10_1101-2021_02_08_430275 103 36 8) 8) CD 10_1101-2021_02_08_430275 103 37 . . . 10_1101-2021_02_08_430275 104 1 Using use VBG 10_1101-2021_02_08_430275 104 2 269 269 CD 10_1101-2021_02_08_430275 104 3 the the DT 10_1101-2021_02_08_430275 104 4 sliding slide VBG 10_1101-2021_02_08_430275 104 5 window window NN 10_1101-2021_02_08_430275 104 6 algorithm algorithm NN 10_1101-2021_02_08_430275 104 7 , , , 10_1101-2021_02_08_430275 104 8 the the DT 10_1101-2021_02_08_430275 104 9 ΔAF δaf NN 10_1101-2021_02_08_430275 104 10 value value NN 10_1101-2021_02_08_430275 104 11 of of IN 10_1101-2021_02_08_430275 104 12 each each DT 10_1101-2021_02_08_430275 104 13 sliding slide VBG 10_1101-2021_02_08_430275 104 14 270 270 CD 10_1101-2021_02_08_430275 104 15 window window NN 10_1101-2021_02_08_430275 104 16 , , , 10_1101-2021_02_08_430275 104 17 the the DT 10_1101-2021_02_08_430275 104 18 average average JJ 10_1101-2021_02_08_430275 104 19 ΔAF δaf NN 10_1101-2021_02_08_430275 104 20 values value NNS 10_1101-2021_02_08_430275 104 21 of of IN 10_1101-2021_02_08_430275 104 22 all all DT 10_1101-2021_02_08_430275 104 23 SNPs snp NNS 10_1101-2021_02_08_430275 104 24 in in IN 10_1101-2021_02_08_430275 104 25 that that DT 10_1101-2021_02_08_430275 104 26 sliding slide VBG 10_1101-2021_02_08_430275 104 27 271 271 CD 10_1101-2021_02_08_430275 104 28 window window NN 10_1101-2021_02_08_430275 104 29 , , , 10_1101-2021_02_08_430275 104 30 was be VBD 10_1101-2021_02_08_430275 104 31 plotted plot VBN 10_1101-2021_02_08_430275 104 32 against against IN 10_1101-2021_02_08_430275 104 33 its -PRON- PRP$ 10_1101-2021_02_08_430275 104 34 genomic genomic JJ 10_1101-2021_02_08_430275 104 35 position position NN 10_1101-2021_02_08_430275 104 36 ( ( -LRB- 10_1101-2021_02_08_430275 104 37 Figure Figure NNP 10_1101-2021_02_08_430275 104 38 1d 1d CD 10_1101-2021_02_08_430275 104 39 ) ) -RRB- 10_1101-2021_02_08_430275 104 40 . . . 10_1101-2021_02_08_430275 105 1 272 272 CD 10_1101-2021_02_08_430275 105 2 A a DT 10_1101-2021_02_08_430275 105 3 significant significant JJ 10_1101-2021_02_08_430275 105 4 peak peak NN 10_1101-2021_02_08_430275 105 5 on on IN 10_1101-2021_02_08_430275 105 6 chromosome chromosome NN 10_1101-2021_02_08_430275 105 7 11 11 CD 10_1101-2021_02_08_430275 105 8 was be VBD 10_1101-2021_02_08_430275 105 9 identified identify VBN 10_1101-2021_02_08_430275 105 10 , , , 10_1101-2021_02_08_430275 105 11 the the DT 10_1101-2021_02_08_430275 105 12 peak peak NN 10_1101-2021_02_08_430275 105 13 273 273 CD 10_1101-2021_02_08_430275 105 14 position position NN 10_1101-2021_02_08_430275 105 15 was be VBD 10_1101-2021_02_08_430275 105 16 located locate VBN 10_1101-2021_02_08_430275 105 17 at at IN 10_1101-2021_02_08_430275 105 18 26.45 26.45 CD 10_1101-2021_02_08_430275 105 19 Mb Mb NNP 10_1101-2021_02_08_430275 105 20 , , , 10_1101-2021_02_08_430275 105 21 its -PRON- PRP$ 10_1101-2021_02_08_430275 105 22 ΔAF δaf NN 10_1101-2021_02_08_430275 105 23 value value NN 10_1101-2021_02_08_430275 105 24 was be VBD 10_1101-2021_02_08_430275 105 25 0.7173 0.7173 CD 10_1101-2021_02_08_430275 105 26 , , , 10_1101-2021_02_08_430275 105 27 274 274 CD 10_1101-2021_02_08_430275 105 28 and and CC 10_1101-2021_02_08_430275 105 29 the the DT 10_1101-2021_02_08_430275 105 30 99 99 CD 10_1101-2021_02_08_430275 105 31 % % NN 10_1101-2021_02_08_430275 105 32 confidence confidence NN 10_1101-2021_02_08_430275 105 33 interval interval NN 10_1101-2021_02_08_430275 105 34 was be VBD 10_1101-2021_02_08_430275 105 35 −0.6508 −0.6508 NNP 10_1101-2021_02_08_430275 105 36 to to IN 10_1101-2021_02_08_430275 105 37 0.6497 0.6497 CD 10_1101-2021_02_08_430275 105 38 . . . 10_1101-2021_02_08_430275 106 1 275 275 CD 10_1101-2021_02_08_430275 106 2 BSA BSA NNP 10_1101-2021_02_08_430275 106 3 - - HYPH 10_1101-2021_02_08_430275 106 4 Seq seq NN 10_1101-2021_02_08_430275 106 5 data data NN 10_1101-2021_02_08_430275 106 6 analysis analysis NN 10_1101-2021_02_08_430275 106 7 using use VBG 10_1101-2021_02_08_430275 106 8 only only RB 10_1101-2021_02_08_430275 106 9 the the DT 10_1101-2021_02_08_430275 106 10 bulk bulk JJ 10_1101-2021_02_08_430275 106 11 genome genome NN 10_1101-2021_02_08_430275 106 12 se- se- NNP 10_1101-2021_02_08_430275 106 13 276 276 CD 10_1101-2021_02_08_430275 106 14 quences quence NNS 10_1101-2021_02_08_430275 106 15 . . . 10_1101-2021_02_08_430275 107 1 The the DT 10_1101-2021_02_08_430275 107 2 SNP SNP NNP 10_1101-2021_02_08_430275 107 3 calling call VBG 10_1101-2021_02_08_430275 107 4 - - HYPH 10_1101-2021_02_08_430275 107 5 generated generate VBN 10_1101-2021_02_08_430275 107 6 bulk bulk JJ 10_1101-2021_02_08_430275 107 7 SNP SNP NNP 10_1101-2021_02_08_430275 107 8 dataset dataset NN 10_1101-2021_02_08_430275 107 9 was be VBD 10_1101-2021_02_08_430275 107 10 277 277 CD 10_1101-2021_02_08_430275 107 11 processed process VBN 10_1101-2021_02_08_430275 107 12 with with IN 10_1101-2021_02_08_430275 107 13 the the DT 10_1101-2021_02_08_430275 107 14 Python Python NNP 10_1101-2021_02_08_430275 107 15 script script NN 10_1101-2021_02_08_430275 107 16 PyBSASeq.py pybsaseq.py NN 10_1101-2021_02_08_430275 107 17 . . . 10_1101-2021_02_08_430275 108 1 All all PDT 10_1101-2021_02_08_430275 108 2 the the DT 10_1101-2021_02_08_430275 108 3 meth- meth- JJ 10_1101-2021_02_08_430275 108 4 278 278 CD 10_1101-2021_02_08_430275 108 5 ods od NNS 10_1101-2021_02_08_430275 108 6 and and CC 10_1101-2021_02_08_430275 108 7 parameters parameter NNS 10_1101-2021_02_08_430275 108 8 were be VBD 10_1101-2021_02_08_430275 108 9 the the DT 10_1101-2021_02_08_430275 108 10 same same JJ 10_1101-2021_02_08_430275 108 11 as as IN 10_1101-2021_02_08_430275 108 12 above above RB 10_1101-2021_02_08_430275 108 13 ; ; : 10_1101-2021_02_08_430275 108 14 the the DT 10_1101-2021_02_08_430275 108 15 only only JJ 10_1101-2021_02_08_430275 108 16 difference difference NN 10_1101-2021_02_08_430275 108 17 279 279 CD 10_1101-2021_02_08_430275 108 18 was be VBD 10_1101-2021_02_08_430275 108 19 that that IN 10_1101-2021_02_08_430275 108 20 the the DT 10_1101-2021_02_08_430275 108 21 parental parental JJ 10_1101-2021_02_08_430275 108 22 SNP SNP NNP 10_1101-2021_02_08_430275 108 23 dataset dataset NN 10_1101-2021_02_08_430275 108 24 was be VBD 10_1101-2021_02_08_430275 108 25 not not RB 10_1101-2021_02_08_430275 108 26 used use VBN 10_1101-2021_02_08_430275 108 27 . . . 10_1101-2021_02_08_430275 109 1 280 280 CD 10_1101-2021_02_08_430275 109 2 The the DT 10_1101-2021_02_08_430275 109 3 significant significant JJ 10_1101-2021_02_08_430275 109 4 structural structural JJ 10_1101-2021_02_08_430275 109 5 variant variant JJ 10_1101-2021_02_08_430275 109 6 method method NN 10_1101-2021_02_08_430275 109 7 : : : 10_1101-2021_02_08_430275 109 8 The the DT 10_1101-2021_02_08_430275 109 9 chromoso- chromoso- JJ 10_1101-2021_02_08_430275 109 10 281 281 CD 10_1101-2021_02_08_430275 109 11 mal mal JJ 10_1101-2021_02_08_430275 109 12 distribution distribution NN 10_1101-2021_02_08_430275 109 13 of of IN 10_1101-2021_02_08_430275 109 14 the the DT 10_1101-2021_02_08_430275 109 15 sSNPs ssnps NN 10_1101-2021_02_08_430275 109 16 and and CC 10_1101-2021_02_08_430275 109 17 total total JJ 10_1101-2021_02_08_430275 109 18 SNPs snp NNS 10_1101-2021_02_08_430275 109 19 are be VBP 10_1101-2021_02_08_430275 109 20 summarized summarize VBN 10_1101-2021_02_08_430275 109 21 282 282 CD 10_1101-2021_02_08_430275 109 22 in in IN 10_1101-2021_02_08_430275 109 23 Table table NN 10_1101-2021_02_08_430275 109 24 2 2 CD 10_1101-2021_02_08_430275 109 25 . . . 10_1101-2021_02_08_430275 110 1 The the DT 10_1101-2021_02_08_430275 110 2 total total JJ 10_1101-2021_02_08_430275 110 3 number number NN 10_1101-2021_02_08_430275 110 4 of of IN 10_1101-2021_02_08_430275 110 5 SNPs snp NNS 10_1101-2021_02_08_430275 110 6 was be VBD 10_1101-2021_02_08_430275 110 7 1 1 CD 10_1101-2021_02_08_430275 110 8 346 346 CD 10_1101-2021_02_08_430275 110 9 185 185 CD 10_1101-2021_02_08_430275 110 10 here here RB 10_1101-2021_02_08_430275 110 11 , , , 10_1101-2021_02_08_430275 110 12 283 283 CD 10_1101-2021_02_08_430275 110 13 much much RB 10_1101-2021_02_08_430275 110 14 higher high JJR 10_1101-2021_02_08_430275 110 15 than than IN 10_1101-2021_02_08_430275 110 16 the the DT 10_1101-2021_02_08_430275 110 17 above above JJ 10_1101-2021_02_08_430275 110 18 , , , 10_1101-2021_02_08_430275 110 19 which which WDT 10_1101-2021_02_08_430275 110 20 was be VBD 10_1101-2021_02_08_430275 110 21 1 1 CD 10_1101-2021_02_08_430275 110 22 099 099 CD 10_1101-2021_02_08_430275 110 23 516 516 CD 10_1101-2021_02_08_430275 110 24 . . . 10_1101-2021_02_08_430275 111 1 The the DT 10_1101-2021_02_08_430275 111 2 ge- ge- NN 10_1101-2021_02_08_430275 111 3 284 284 CD 10_1101-2021_02_08_430275 111 4 nomic nomic JJ 10_1101-2021_02_08_430275 111 5 distribution distribution NN 10_1101-2021_02_08_430275 111 6 of of IN 10_1101-2021_02_08_430275 111 7 the the DT 10_1101-2021_02_08_430275 111 8 sSNPs ssnps CD 10_1101-2021_02_08_430275 111 9 , , , 10_1101-2021_02_08_430275 111 10 the the DT 10_1101-2021_02_08_430275 111 11 total total JJ 10_1101-2021_02_08_430275 111 12 SNPs snp NNS 10_1101-2021_02_08_430275 111 13 , , , 10_1101-2021_02_08_430275 111 14 and and CC 10_1101-2021_02_08_430275 111 15 the the DT 10_1101-2021_02_08_430275 111 16 285 285 CD 10_1101-2021_02_08_430275 111 17 sSNP ssnp NN 10_1101-2021_02_08_430275 111 18 / / SYM 10_1101-2021_02_08_430275 111 19 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 111 20 ratios ratio NNS 10_1101-2021_02_08_430275 111 21 of of IN 10_1101-2021_02_08_430275 111 22 the the DT 10_1101-2021_02_08_430275 111 23 sliding slide VBG 10_1101-2021_02_08_430275 111 24 windows window NNS 10_1101-2021_02_08_430275 111 25 are be VBP 10_1101-2021_02_08_430275 111 26 presented present VBN 10_1101-2021_02_08_430275 111 27 286 286 CD 10_1101-2021_02_08_430275 111 28 in in IN 10_1101-2021_02_08_430275 111 29 Figure Figure NNP 10_1101-2021_02_08_430275 111 30 2a 2a NNP 10_1101-2021_02_08_430275 111 31 and and CC 10_1101-2021_02_08_430275 111 32 Figure Figure NNP 10_1101-2021_02_08_430275 111 33 2b 2b NNS 10_1101-2021_02_08_430275 111 34 . . . 10_1101-2021_02_08_430275 112 1 The the DT 10_1101-2021_02_08_430275 112 2 patterns pattern NNS 10_1101-2021_02_08_430275 112 3 of of IN 10_1101-2021_02_08_430275 112 4 the the DT 10_1101-2021_02_08_430275 112 5 curves curve NNS 10_1101-2021_02_08_430275 112 6 were be VBD 10_1101-2021_02_08_430275 112 7 287 287 CD 10_1101-2021_02_08_430275 112 8 very very RB 10_1101-2021_02_08_430275 112 9 similar similar JJ 10_1101-2021_02_08_430275 112 10 to to IN 10_1101-2021_02_08_430275 112 11 those those DT 10_1101-2021_02_08_430275 112 12 in in IN 10_1101-2021_02_08_430275 112 13 Figure Figure NNP 10_1101-2021_02_08_430275 112 14 1a 1a NNP 10_1101-2021_02_08_430275 112 15 and and CC 10_1101-2021_02_08_430275 112 16 Figure Figure NNP 10_1101-2021_02_08_430275 112 17 1b 1b NN 10_1101-2021_02_08_430275 112 18 . . . 10_1101-2021_02_08_430275 113 1 One one CD 10_1101-2021_02_08_430275 113 2 of of IN 10_1101-2021_02_08_430275 113 3 the the DT 10_1101-2021_02_08_430275 113 4 288 288 CD 10_1101-2021_02_08_430275 113 5 obvious obvious JJ 10_1101-2021_02_08_430275 113 6 differences difference NNS 10_1101-2021_02_08_430275 113 7 was be VBD 10_1101-2021_02_08_430275 113 8 that that DT 10_1101-2021_02_08_430275 113 9 sSNP ssnp NN 10_1101-2021_02_08_430275 113 10 / / SYM 10_1101-2021_02_08_430275 113 11 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 113 12 ratios ratio NNS 10_1101-2021_02_08_430275 113 13 of of IN 10_1101-2021_02_08_430275 113 14 the the DT 10_1101-2021_02_08_430275 113 15 slid- slid- NN 10_1101-2021_02_08_430275 113 16 289 289 CD 10_1101-2021_02_08_430275 113 17 ing e VBG 10_1101-2021_02_08_430275 113 18 windows window NNS 10_1101-2021_02_08_430275 113 19 were be VBD 10_1101-2021_02_08_430275 113 20 much much RB 10_1101-2021_02_08_430275 113 21 lower low JJR 10_1101-2021_02_08_430275 113 22 than than IN 10_1101-2021_02_08_430275 113 23 those those DT 10_1101-2021_02_08_430275 113 24 in in IN 10_1101-2021_02_08_430275 113 25 Figure Figure NNP 10_1101-2021_02_08_430275 113 26 1b 1b NN 10_1101-2021_02_08_430275 113 27 , , , 10_1101-2021_02_08_430275 113 28 leading lead VBG 10_1101-2021_02_08_430275 113 29 290 290 CD 10_1101-2021_02_08_430275 113 30 to to IN 10_1101-2021_02_08_430275 113 31 missing miss VBG 10_1101-2021_02_08_430275 113 32 the the DT 10_1101-2021_02_08_430275 113 33 minor minor JJ 10_1101-2021_02_08_430275 113 34 locus locus NN 10_1101-2021_02_08_430275 113 35 on on IN 10_1101-2021_02_08_430275 113 36 chromosome chromosome NN 10_1101-2021_02_08_430275 113 37 9 9 CD 10_1101-2021_02_08_430275 113 38 . . . 10_1101-2021_02_08_430275 114 1 Only only RB 10_1101-2021_02_08_430275 114 2 the the DT 10_1101-2021_02_08_430275 114 3 peak peak NN 10_1101-2021_02_08_430275 114 4 291 291 CD 10_1101-2021_02_08_430275 114 5 on on IN 10_1101-2021_02_08_430275 114 6 chromosome chromosome NN 10_1101-2021_02_08_430275 114 7 11 11 CD 10_1101-2021_02_08_430275 114 8 was be VBD 10_1101-2021_02_08_430275 114 9 significant significant JJ 10_1101-2021_02_08_430275 114 10 ; ; : 10_1101-2021_02_08_430275 114 11 it -PRON- PRP 10_1101-2021_02_08_430275 114 12 was be VBD 10_1101-2021_02_08_430275 114 13 located locate VBN 10_1101-2021_02_08_430275 114 14 at at IN 10_1101-2021_02_08_430275 114 15 26.96 26.96 CD 10_1101-2021_02_08_430275 114 16 Mb Mb NNP 10_1101-2021_02_08_430275 114 17 , , , 10_1101-2021_02_08_430275 114 18 292 292 CD 10_1101-2021_02_08_430275 114 19 a a DT 10_1101-2021_02_08_430275 114 20 520 520 CD 10_1101-2021_02_08_430275 114 21 kb kb NN 10_1101-2021_02_08_430275 114 22 shift shift NN 10_1101-2021_02_08_430275 114 23 compared compare VBN 10_1101-2021_02_08_430275 114 24 to to IN 10_1101-2021_02_08_430275 114 25 Figure figure NN 10_1101-2021_02_08_430275 114 26 1b 1b NN 10_1101-2021_02_08_430275 114 27 . . . 10_1101-2021_02_08_430275 115 1 The the DT 10_1101-2021_02_08_430275 115 2 sliding slide VBG 10_1101-2021_02_08_430275 115 3 window window NN 10_1101-2021_02_08_430275 115 4 293 293 CD 10_1101-2021_02_08_430275 115 5 contained contain VBD 10_1101-2021_02_08_430275 115 6 1122 1122 CD 10_1101-2021_02_08_430275 115 7 sSNPs ssnps CD 10_1101-2021_02_08_430275 115 8 and and CC 10_1101-2021_02_08_430275 115 9 2945 2945 CD 10_1101-2021_02_08_430275 115 10 total total JJ 10_1101-2021_02_08_430275 115 11 SNPs snp NNS 10_1101-2021_02_08_430275 115 12 , , , 10_1101-2021_02_08_430275 115 13 corresponding correspond VBG 10_1101-2021_02_08_430275 115 14 to to IN 10_1101-2021_02_08_430275 115 15 294 294 CD 10_1101-2021_02_08_430275 115 16 a a DT 10_1101-2021_02_08_430275 115 17 0.3810 0.3810 CD 10_1101-2021_02_08_430275 115 18 sSNP ssnp NN 10_1101-2021_02_08_430275 115 19 / / SYM 10_1101-2021_02_08_430275 115 20 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 115 21 ratio ratio NN 10_1101-2021_02_08_430275 115 22 , , , 10_1101-2021_02_08_430275 115 23 well well RB 10_1101-2021_02_08_430275 115 24 above above IN 10_1101-2021_02_08_430275 115 25 the the DT 10_1101-2021_02_08_430275 115 26 genome genome NN 10_1101-2021_02_08_430275 115 27 - - HYPH 10_1101-2021_02_08_430275 115 28 wide wide JJ 10_1101-2021_02_08_430275 115 29 295 295 CD 10_1101-2021_02_08_430275 115 30 threshold threshold NN 10_1101-2021_02_08_430275 115 31 ( ( -LRB- 10_1101-2021_02_08_430275 115 32 0.0535 0.0535 CD 10_1101-2021_02_08_430275 115 33 ) ) -RRB- 10_1101-2021_02_08_430275 115 34 and and CC 10_1101-2021_02_08_430275 115 35 the the DT 10_1101-2021_02_08_430275 115 36 sliding slide VBG 10_1101-2021_02_08_430275 115 37 window window NN 10_1101-2021_02_08_430275 115 38 specific specific NN 10_1101-2021_02_08_430275 115 39 threshold threshold NN 10_1101-2021_02_08_430275 115 40 296 296 CD 10_1101-2021_02_08_430275 115 41 ( ( -LRB- 10_1101-2021_02_08_430275 115 42 0.0601 0.0601 CD 10_1101-2021_02_08_430275 115 43 ) ) -RRB- 10_1101-2021_02_08_430275 115 44 . . . 10_1101-2021_02_08_430275 116 1 The the DT 10_1101-2021_02_08_430275 116 2 average average JJ 10_1101-2021_02_08_430275 116 3 SNPs snp NNS 10_1101-2021_02_08_430275 116 4 per per IN 10_1101-2021_02_08_430275 116 5 sliding slide VBG 10_1101-2021_02_08_430275 116 6 window window NN 10_1101-2021_02_08_430275 116 7 was be VBD 10_1101-2021_02_08_430275 116 8 7215 7215 CD 10_1101-2021_02_08_430275 116 9 . . . 10_1101-2021_02_08_430275 117 1 297 297 CD 10_1101-2021_02_08_430275 117 2 The the DT 10_1101-2021_02_08_430275 117 3 G g NN 10_1101-2021_02_08_430275 117 4 - - HYPH 10_1101-2021_02_08_430275 117 5 statistic statistic NN 10_1101-2021_02_08_430275 117 6 method method NN 10_1101-2021_02_08_430275 117 7 : : : 10_1101-2021_02_08_430275 117 8 The the DT 10_1101-2021_02_08_430275 117 9 patterns pattern NNS 10_1101-2021_02_08_430275 117 10 of of IN 10_1101-2021_02_08_430275 117 11 the the DT 10_1101-2021_02_08_430275 117 12 G g NN 10_1101-2021_02_08_430275 117 13 - - HYPH 10_1101-2021_02_08_430275 117 14 statistic statistic NNP 10_1101-2021_02_08_430275 117 15 298 298 CD 10_1101-2021_02_08_430275 117 16 value value NN 10_1101-2021_02_08_430275 117 17 plot plot NN 10_1101-2021_02_08_430275 117 18 ( ( -LRB- 10_1101-2021_02_08_430275 117 19 Figure Figure NNP 10_1101-2021_02_08_430275 117 20 2c 2c NN 10_1101-2021_02_08_430275 117 21 ) ) -RRB- 10_1101-2021_02_08_430275 117 22 were be VBD 10_1101-2021_02_08_430275 117 23 very very RB 10_1101-2021_02_08_430275 117 24 similar similar JJ 10_1101-2021_02_08_430275 117 25 to to IN 10_1101-2021_02_08_430275 117 26 that that DT 10_1101-2021_02_08_430275 117 27 in in IN 10_1101-2021_02_08_430275 117 28 Figure Figure NNP 10_1101-2021_02_08_430275 117 29 1c 1c CD 10_1101-2021_02_08_430275 117 30 , , , 10_1101-2021_02_08_430275 117 31 299 299 CD 10_1101-2021_02_08_430275 117 32 but but CC 10_1101-2021_02_08_430275 117 33 the the DT 10_1101-2021_02_08_430275 117 34 G g NN 10_1101-2021_02_08_430275 117 35 - - HYPH 10_1101-2021_02_08_430275 117 36 statistic statistic NN 10_1101-2021_02_08_430275 117 37 values value NNS 10_1101-2021_02_08_430275 117 38 were be VBD 10_1101-2021_02_08_430275 117 39 significantly significantly RB 10_1101-2021_02_08_430275 117 40 lower low JJR 10_1101-2021_02_08_430275 117 41 than than IN 10_1101-2021_02_08_430275 117 42 those those DT 10_1101-2021_02_08_430275 117 43 300 300 CD 10_1101-2021_02_08_430275 117 44 in in IN 10_1101-2021_02_08_430275 117 45 Figure Figure NNP 10_1101-2021_02_08_430275 117 46 1c 1c NNP 10_1101-2021_02_08_430275 117 47 , , , 10_1101-2021_02_08_430275 117 48 and and CC 10_1101-2021_02_08_430275 117 49 the the DT 10_1101-2021_02_08_430275 117 50 threshold threshold NN 10_1101-2021_02_08_430275 117 51 did do VBD 10_1101-2021_02_08_430275 117 52 not not RB 10_1101-2021_02_08_430275 117 53 change change VB 10_1101-2021_02_08_430275 117 54 much much JJ 10_1101-2021_02_08_430275 117 55 . . . 10_1101-2021_02_08_430275 118 1 Only only RB 10_1101-2021_02_08_430275 118 2 301 301 CD 10_1101-2021_02_08_430275 118 3 a a DT 10_1101-2021_02_08_430275 118 4 single single JJ 10_1101-2021_02_08_430275 118 5 sliding slide VBG 10_1101-2021_02_08_430275 118 6 window window NN 10_1101-2021_02_08_430275 118 7 was be VBD 10_1101-2021_02_08_430275 118 8 above above IN 10_1101-2021_02_08_430275 118 9 the the DT 10_1101-2021_02_08_430275 118 10 threshold threshold NN 10_1101-2021_02_08_430275 118 11 ( ( -LRB- 10_1101-2021_02_08_430275 118 12 8.8953 8.8953 CD 10_1101-2021_02_08_430275 118 13 ) ) -RRB- 10_1101-2021_02_08_430275 118 14 , , , 10_1101-2021_02_08_430275 118 15 its -PRON- PRP$ 10_1101-2021_02_08_430275 118 16 302 302 CD 10_1101-2021_02_08_430275 118 17 position position NN 10_1101-2021_02_08_430275 118 18 was be VBD 10_1101-2021_02_08_430275 118 19 at at IN 10_1101-2021_02_08_430275 118 20 29.96 29.96 CD 10_1101-2021_02_08_430275 118 21 Mb Mb NNP 10_1101-2021_02_08_430275 118 22 , , , 10_1101-2021_02_08_430275 118 23 and and CC 10_1101-2021_02_08_430275 118 24 its -PRON- PRP$ 10_1101-2021_02_08_430275 118 25 G g NN 10_1101-2021_02_08_430275 118 26 - - HYPH 10_1101-2021_02_08_430275 118 27 statistic statistic NN 10_1101-2021_02_08_430275 118 28 value value NN 10_1101-2021_02_08_430275 118 29 was be VBD 10_1101-2021_02_08_430275 118 30 8.9060 8.9060 CD 10_1101-2021_02_08_430275 118 31 . . . 10_1101-2021_02_08_430275 119 1 303 303 CD 10_1101-2021_02_08_430275 119 2 The the DT 10_1101-2021_02_08_430275 119 3 allele allele NN 10_1101-2021_02_08_430275 119 4 frequency frequency NN 10_1101-2021_02_08_430275 119 5 method method NN 10_1101-2021_02_08_430275 119 6 : : : 10_1101-2021_02_08_430275 119 7 Without without IN 10_1101-2021_02_08_430275 119 8 the the DT 10_1101-2021_02_08_430275 119 9 aid aid NN 10_1101-2021_02_08_430275 119 10 of of IN 10_1101-2021_02_08_430275 119 11 the the DT 10_1101-2021_02_08_430275 119 12 304 304 CD 10_1101-2021_02_08_430275 119 13 parental parental JJ 10_1101-2021_02_08_430275 119 14 genome genome NN 10_1101-2021_02_08_430275 119 15 sequences sequence NNS 10_1101-2021_02_08_430275 119 16 , , , 10_1101-2021_02_08_430275 119 17 the the DT 10_1101-2021_02_08_430275 119 18 pattern pattern NN 10_1101-2021_02_08_430275 119 19 of of IN 10_1101-2021_02_08_430275 119 20 the the DT 10_1101-2021_02_08_430275 119 21 ΔAF δaf NN 10_1101-2021_02_08_430275 119 22 curve curve NN 10_1101-2021_02_08_430275 119 23 305 305 CD 10_1101-2021_02_08_430275 119 24 of of IN 10_1101-2021_02_08_430275 119 25 chromosome chromosome NN 10_1101-2021_02_08_430275 119 26 11 11 CD 10_1101-2021_02_08_430275 119 27 ( ( -LRB- 10_1101-2021_02_08_430275 119 28 Figure Figure NNP 10_1101-2021_02_08_430275 119 29 2d 2d NNP 10_1101-2021_02_08_430275 119 30 ) ) -RRB- 10_1101-2021_02_08_430275 119 31 , , , 10_1101-2021_02_08_430275 119 32 especially especially RB 10_1101-2021_02_08_430275 119 33 the the DT 10_1101-2021_02_08_430275 119 34 genomic genomic JJ 10_1101-2021_02_08_430275 119 35 region region NN 10_1101-2021_02_08_430275 119 36 306 306 CD 10_1101-2021_02_08_430275 119 37 associated associate VBN 10_1101-2021_02_08_430275 119 38 with with IN 10_1101-2021_02_08_430275 119 39 the the DT 10_1101-2021_02_08_430275 119 40 trait trait NN 10_1101-2021_02_08_430275 119 41 , , , 10_1101-2021_02_08_430275 119 42 was be VBD 10_1101-2021_02_08_430275 119 43 drastically drastically RB 10_1101-2021_02_08_430275 119 44 different different JJ 10_1101-2021_02_08_430275 119 45 from from IN 10_1101-2021_02_08_430275 119 46 that that DT 10_1101-2021_02_08_430275 119 47 in in IN 10_1101-2021_02_08_430275 119 48 307 307 CD 10_1101-2021_02_08_430275 119 49 Figure Figure NNP 10_1101-2021_02_08_430275 119 50 1d 1d NN 10_1101-2021_02_08_430275 119 51 . . . 10_1101-2021_02_08_430275 120 1 Differences difference NNS 10_1101-2021_02_08_430275 120 2 in in IN 10_1101-2021_02_08_430275 120 3 the the DT 10_1101-2021_02_08_430275 120 4 curve curve NN 10_1101-2021_02_08_430275 120 5 patterns pattern NNS 10_1101-2021_02_08_430275 120 6 were be VBD 10_1101-2021_02_08_430275 120 7 observed observe VBN 10_1101-2021_02_08_430275 120 8 in in IN 10_1101-2021_02_08_430275 120 9 308 308 CD 10_1101-2021_02_08_430275 120 10 other other JJ 10_1101-2021_02_08_430275 120 11 chromosomes chromosome NNS 10_1101-2021_02_08_430275 120 12 as as RB 10_1101-2021_02_08_430275 120 13 well well RB 10_1101-2021_02_08_430275 120 14 , , , 10_1101-2021_02_08_430275 120 15 but but CC 10_1101-2021_02_08_430275 120 16 they -PRON- PRP 10_1101-2021_02_08_430275 120 17 were be VBD 10_1101-2021_02_08_430275 120 18 relatively relatively RB 10_1101-2021_02_08_430275 120 19 minor minor JJ 10_1101-2021_02_08_430275 120 20 . . . 10_1101-2021_02_08_430275 121 1 All all DT 10_1101-2021_02_08_430275 121 2 309 309 CD 10_1101-2021_02_08_430275 121 3 ΔAF δaf NN 10_1101-2021_02_08_430275 121 4 values value NNS 10_1101-2021_02_08_430275 121 5 were be VBD 10_1101-2021_02_08_430275 121 6 within within IN 10_1101-2021_02_08_430275 121 7 the the DT 10_1101-2021_02_08_430275 121 8 99 99 CD 10_1101-2021_02_08_430275 121 9 % % NN 10_1101-2021_02_08_430275 121 10 confidence confidence NN 10_1101-2021_02_08_430275 121 11 interval interval NN 10_1101-2021_02_08_430275 121 12 , , , 10_1101-2021_02_08_430275 121 13 although although IN 10_1101-2021_02_08_430275 121 14 310 310 CD 10_1101-2021_02_08_430275 121 15 Zhang Zhang NNP 10_1101-2021_02_08_430275 121 16 et et NNP 10_1101-2021_02_08_430275 121 17 al al NNP 10_1101-2021_02_08_430275 121 18 . . . 10_1101-2021_02_08_430275 122 1 bioRχiv bioRχiv NNP 10_1101-2021_02_08_430275 122 2 | | NNP 10_1101-2021_02_08_430275 122 3 November November NNP 10_1101-2021_02_08_430275 122 4 24 24 CD 10_1101-2021_02_08_430275 122 5 , , , 10_1101-2021_02_08_430275 122 6 2020 2020 CD 10_1101-2021_02_08_430275 122 7 | | SYM 10_1101-2021_02_08_430275 122 8 3 3 CD 10_1101-2021_02_08_430275 122 9 .CC .CC : 10_1101-2021_02_08_430275 122 10 - - HYPH 10_1101-2021_02_08_430275 122 11 BY by IN 10_1101-2021_02_08_430275 122 12 - - HYPH 10_1101-2021_02_08_430275 122 13 NC NC NNP 10_1101-2021_02_08_430275 122 14 - - HYPH 10_1101-2021_02_08_430275 122 15 ND ND NNP 10_1101-2021_02_08_430275 122 16 4.0 4.0 CD 10_1101-2021_02_08_430275 122 17 International International NNP 10_1101-2021_02_08_430275 122 18 licenseavailable licenseavailable NN 10_1101-2021_02_08_430275 122 19 under under IN 10_1101-2021_02_08_430275 122 20 a a DT 10_1101-2021_02_08_430275 122 21 ( ( -LRB- 10_1101-2021_02_08_430275 122 22 which which WDT 10_1101-2021_02_08_430275 122 23 was be VBD 10_1101-2021_02_08_430275 122 24 not not RB 10_1101-2021_02_08_430275 122 25 certified certify VBN 10_1101-2021_02_08_430275 122 26 by by IN 10_1101-2021_02_08_430275 122 27 peer peer NN 10_1101-2021_02_08_430275 122 28 review review NN 10_1101-2021_02_08_430275 122 29 ) ) -RRB- 10_1101-2021_02_08_430275 122 30 is be VBZ 10_1101-2021_02_08_430275 122 31 the the DT 10_1101-2021_02_08_430275 122 32 author author NN 10_1101-2021_02_08_430275 122 33 / / SYM 10_1101-2021_02_08_430275 122 34 funder funder NN 10_1101-2021_02_08_430275 122 35 , , , 10_1101-2021_02_08_430275 122 36 who who WP 10_1101-2021_02_08_430275 122 37 has have VBZ 10_1101-2021_02_08_430275 122 38 granted grant VBN 10_1101-2021_02_08_430275 122 39 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 122 40 a a DT 10_1101-2021_02_08_430275 122 41 license license NN 10_1101-2021_02_08_430275 122 42 to to TO 10_1101-2021_02_08_430275 122 43 display display VB 10_1101-2021_02_08_430275 122 44 the the DT 10_1101-2021_02_08_430275 122 45 preprint preprint NN 10_1101-2021_02_08_430275 122 46 in in IN 10_1101-2021_02_08_430275 122 47 perpetuity perpetuity NN 10_1101-2021_02_08_430275 122 48 . . . 10_1101-2021_02_08_430275 123 1 It -PRON- PRP 10_1101-2021_02_08_430275 123 2 is be VBZ 10_1101-2021_02_08_430275 123 3 made make VBN 10_1101-2021_02_08_430275 123 4 The the DT 10_1101-2021_02_08_430275 123 5 copyright copyright NN 10_1101-2021_02_08_430275 123 6 holder holder NN 10_1101-2021_02_08_430275 123 7 for for IN 10_1101-2021_02_08_430275 123 8 this this DT 10_1101-2021_02_08_430275 123 9 preprintthis preprintthis NN 10_1101-2021_02_08_430275 123 10 version version NN 10_1101-2021_02_08_430275 123 11 posted post VBD 10_1101-2021_02_08_430275 123 12 February February NNP 10_1101-2021_02_08_430275 123 13 10 10 CD 10_1101-2021_02_08_430275 123 14 , , , 10_1101-2021_02_08_430275 123 15 2021 2021 CD 10_1101-2021_02_08_430275 123 16 . . . 10_1101-2021_02_08_430275 123 17 ; ; : 10_1101-2021_02_08_430275 123 18 https://doi.org/10.1101/2021.02.08.430275doi https://doi.org/10.1101/2021.02.08.430275doi ADD 10_1101-2021_02_08_430275 123 19 : : : 10_1101-2021_02_08_430275 123 20 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 123 21 preprint preprint NN 10_1101-2021_02_08_430275 123 22 https://github.com/dblhlx/PyBSASeq/blob/master/PyBSASeq_WP.py https://github.com/dblhlx/pybsaseq/blob/master/pybsaseq_wp.py ADD 10_1101-2021_02_08_430275 123 23 https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels- https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels- NNPS 10_1101-2021_02_08_430275 123 24 https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels- https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels- NNPS 10_1101-2021_02_08_430275 123 25 https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels- https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels- NNPS 10_1101-2021_02_08_430275 123 26 https://github.com/dblhlx/PyBSASeq/blob/master/PyBSASeq.py https://github.com/dblhlx/pybsaseq/blob/master/pybsaseq.py FW 10_1101-2021_02_08_430275 123 27 https://doi.org/10.1101/2021.02.08.430275 https://doi.org/10.1101/2021.02.08.430275 NNP 10_1101-2021_02_08_430275 123 28 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_02_08_430275 123 29 DR DR NNP 10_1101-2021_02_08_430275 123 30 AF AF NNP 10_1101-2021_02_08_430275 123 31 T t NN 10_1101-2021_02_08_430275 123 32 0 0 CD 10_1101-2021_02_08_430275 123 33 2000 2000 CD 10_1101-2021_02_08_430275 123 34 4000 4000 CD 10_1101-2021_02_08_430275 123 35 6000 6000 CD 10_1101-2021_02_08_430275 123 36 8000 8000 CD 10_1101-2021_02_08_430275 123 37 10000 10000 CD 10_1101-2021_02_08_430275 123 38 N n CD 10_1101-2021_02_08_430275 123 39 um um UH 10_1101-2021_02_08_430275 123 40 be be VB 10_1101-2021_02_08_430275 123 41 r r NN 10_1101-2021_02_08_430275 123 42 o o NN 10_1101-2021_02_08_430275 123 43 f f NN 10_1101-2021_02_08_430275 123 44 S s NN 10_1101-2021_02_08_430275 123 45 N N NNP 10_1101-2021_02_08_430275 123 46 P P NNP 10_1101-2021_02_08_430275 123 47 s s NN 10_1101-2021_02_08_430275 123 48 Chr1 Chr1 NNPS 10_1101-2021_02_08_430275 123 49 Chr2 Chr2 NNP 10_1101-2021_02_08_430275 123 50 Chr3 chr3 NN 10_1101-2021_02_08_430275 123 51 Chr4 chr4 NN 10_1101-2021_02_08_430275 123 52 Chr5 Chr5 VBZ 10_1101-2021_02_08_430275 123 53 Chr6 Chr6 NNP 10_1101-2021_02_08_430275 123 54 Chr7 Chr7 NNP 10_1101-2021_02_08_430275 123 55 Chr8 Chr8 NNP 10_1101-2021_02_08_430275 123 56 Chr9 Chr9 NNP 10_1101-2021_02_08_430275 123 57 Chr10 Chr10 NNP 10_1101-2021_02_08_430275 123 58 Chr11 Chr11 NNP 10_1101-2021_02_08_430275 123 59 Chr12 Chr12 NNP 10_1101-2021_02_08_430275 123 60 0.0 0.0 CD 10_1101-2021_02_08_430275 123 61 0.2 0.2 CD 10_1101-2021_02_08_430275 123 62 0.4 0.4 CD 10_1101-2021_02_08_430275 123 63 0.6 0.6 CD 10_1101-2021_02_08_430275 123 64 sS ss NN 10_1101-2021_02_08_430275 123 65 N N NNP 10_1101-2021_02_08_430275 123 66 P P NNP 10_1101-2021_02_08_430275 123 67 /to /to : 10_1101-2021_02_08_430275 123 68 ta ta ADD 10_1101-2021_02_08_430275 123 69 lS lS NNP 10_1101-2021_02_08_430275 123 70 N n CC 10_1101-2021_02_08_430275 123 71 P P NNP 10_1101-2021_02_08_430275 123 72 0 0 CD 10_1101-2021_02_08_430275 123 73 5 5 CD 10_1101-2021_02_08_430275 123 74 10 10 CD 10_1101-2021_02_08_430275 123 75 G g NN 10_1101-2021_02_08_430275 123 76 -s -s : 10_1101-2021_02_08_430275 123 77 ta ta NNP 10_1101-2021_02_08_430275 123 78 tis tis NNP 10_1101-2021_02_08_430275 123 79 tic tic NNP 10_1101-2021_02_08_430275 123 80 0 0 CD 10_1101-2021_02_08_430275 123 81 1 1 CD 10_1101-2021_02_08_430275 123 82 2 2 CD 10_1101-2021_02_08_430275 123 83 3 3 CD 10_1101-2021_02_08_430275 123 84 4 4 CD 10_1101-2021_02_08_430275 123 85 0.5 0.5 CD 10_1101-2021_02_08_430275 123 86 0.0 0.0 CD 10_1101-2021_02_08_430275 123 87 0.5 0.5 CD 10_1101-2021_02_08_430275 123 88 A a NN 10_1101-2021_02_08_430275 123 89 F f NN 10_1101-2021_02_08_430275 123 90 0 0 CD 10_1101-2021_02_08_430275 123 91 1 1 CD 10_1101-2021_02_08_430275 123 92 2 2 CD 10_1101-2021_02_08_430275 123 93 3 3 CD 10_1101-2021_02_08_430275 123 94 0 0 CD 10_1101-2021_02_08_430275 123 95 1 1 CD 10_1101-2021_02_08_430275 123 96 2 2 CD 10_1101-2021_02_08_430275 123 97 3 3 CD 10_1101-2021_02_08_430275 123 98 0 0 CD 10_1101-2021_02_08_430275 123 99 1 1 CD 10_1101-2021_02_08_430275 123 100 2 2 CD 10_1101-2021_02_08_430275 123 101 3 3 CD 10_1101-2021_02_08_430275 123 102 0 0 CD 10_1101-2021_02_08_430275 123 103 1 1 CD 10_1101-2021_02_08_430275 123 104 2 2 CD 10_1101-2021_02_08_430275 123 105 0 0 CD 10_1101-2021_02_08_430275 123 106 1 1 CD 10_1101-2021_02_08_430275 123 107 2 2 CD 10_1101-2021_02_08_430275 123 108 0 0 CD 10_1101-2021_02_08_430275 123 109 1 1 CD 10_1101-2021_02_08_430275 123 110 2 2 CD 10_1101-2021_02_08_430275 123 111 0 0 CD 10_1101-2021_02_08_430275 123 112 1 1 CD 10_1101-2021_02_08_430275 123 113 2 2 CD 10_1101-2021_02_08_430275 123 114 0 0 CD 10_1101-2021_02_08_430275 123 115 1 1 CD 10_1101-2021_02_08_430275 123 116 2 2 CD 10_1101-2021_02_08_430275 123 117 0 0 CD 10_1101-2021_02_08_430275 123 118 1 1 CD 10_1101-2021_02_08_430275 123 119 2 2 CD 10_1101-2021_02_08_430275 123 120 0 0 CD 10_1101-2021_02_08_430275 123 121 1 1 CD 10_1101-2021_02_08_430275 123 122 2 2 CD 10_1101-2021_02_08_430275 123 123 0 0 CD 10_1101-2021_02_08_430275 123 124 1 1 CD 10_1101-2021_02_08_430275 123 125 2 2 CD 10_1101-2021_02_08_430275 123 126 Genomic genomic JJ 10_1101-2021_02_08_430275 123 127 position position NN 10_1101-2021_02_08_430275 123 128 ( ( -LRB- 10_1101-2021_02_08_430275 123 129 ×10 ×10 NFP 10_1101-2021_02_08_430275 123 130 Mb mb NN 10_1101-2021_02_08_430275 123 131 ) ) -RRB- 10_1101-2021_02_08_430275 123 132 A A NNP 10_1101-2021_02_08_430275 123 133 B b NN 10_1101-2021_02_08_430275 123 134 C c NN 10_1101-2021_02_08_430275 123 135 D d NN 10_1101-2021_02_08_430275 123 136 Figure figure NN 10_1101-2021_02_08_430275 123 137 1 1 CD 10_1101-2021_02_08_430275 123 138 . . . 10_1101-2021_02_08_430275 124 1 BSA BSA NNP 10_1101-2021_02_08_430275 124 2 - - HYPH 10_1101-2021_02_08_430275 124 3 Seq seq NN 10_1101-2021_02_08_430275 124 4 data data NN 10_1101-2021_02_08_430275 124 5 analysis analysis NN 10_1101-2021_02_08_430275 124 6 using use VBG 10_1101-2021_02_08_430275 124 7 the the DT 10_1101-2021_02_08_430275 124 8 genome genome JJ 10_1101-2021_02_08_430275 124 9 sequences sequence NNS 10_1101-2021_02_08_430275 124 10 of of IN 10_1101-2021_02_08_430275 124 11 both both PDT 10_1101-2021_02_08_430275 124 12 the the DT 10_1101-2021_02_08_430275 124 13 parents parent NNS 10_1101-2021_02_08_430275 124 14 and and CC 10_1101-2021_02_08_430275 124 15 the the DT 10_1101-2021_02_08_430275 124 16 bulks bulk NNS 10_1101-2021_02_08_430275 124 17 . . . 10_1101-2021_02_08_430275 125 1 The the DT 10_1101-2021_02_08_430275 125 2 red red JJ 10_1101-2021_02_08_430275 125 3 lines line NNS 10_1101-2021_02_08_430275 125 4 / / SYM 10_1101-2021_02_08_430275 125 5 curves curve NNS 10_1101-2021_02_08_430275 125 6 are be VBP 10_1101-2021_02_08_430275 125 7 the the DT 10_1101-2021_02_08_430275 125 8 thresholds threshold NNS 10_1101-2021_02_08_430275 125 9 . . . 10_1101-2021_02_08_430275 126 1 ( ( -LRB- 10_1101-2021_02_08_430275 126 2 A a DT 10_1101-2021_02_08_430275 126 3 ) ) -RRB- 10_1101-2021_02_08_430275 126 4 Genomic genomic JJ 10_1101-2021_02_08_430275 126 5 distributions distribution NNS 10_1101-2021_02_08_430275 126 6 of of IN 10_1101-2021_02_08_430275 126 7 sSNPs ssnps CD 10_1101-2021_02_08_430275 126 8 ( ( -LRB- 10_1101-2021_02_08_430275 126 9 blue blue NNP 10_1101-2021_02_08_430275 126 10 ) ) -RRB- 10_1101-2021_02_08_430275 126 11 and and CC 10_1101-2021_02_08_430275 126 12 totalSNPs totalsnps RB 10_1101-2021_02_08_430275 126 13 ( ( -LRB- 10_1101-2021_02_08_430275 126 14 black black JJ 10_1101-2021_02_08_430275 126 15 ) ) -RRB- 10_1101-2021_02_08_430275 126 16 . . . 10_1101-2021_02_08_430275 127 1 ( ( -LRB- 10_1101-2021_02_08_430275 127 2 B b NN 10_1101-2021_02_08_430275 127 3 ) ) -RRB- 10_1101-2021_02_08_430275 127 4 Genomic genomic JJ 10_1101-2021_02_08_430275 127 5 distributions distribution NNS 10_1101-2021_02_08_430275 127 6 of of IN 10_1101-2021_02_08_430275 127 7 sSNP ssnp NN 10_1101-2021_02_08_430275 127 8 / / SYM 10_1101-2021_02_08_430275 127 9 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 127 10 ratios ratio NNS 10_1101-2021_02_08_430275 127 11 . . . 10_1101-2021_02_08_430275 128 1 ( ( -LRB- 10_1101-2021_02_08_430275 128 2 C C NNP 10_1101-2021_02_08_430275 128 3 ) ) -RRB- 10_1101-2021_02_08_430275 128 4 Genomic genomic JJ 10_1101-2021_02_08_430275 128 5 distributions distribution NNS 10_1101-2021_02_08_430275 128 6 of of IN 10_1101-2021_02_08_430275 128 7 G g NN 10_1101-2021_02_08_430275 128 8 - - HYPH 10_1101-2021_02_08_430275 128 9 statistic statistic NN 10_1101-2021_02_08_430275 128 10 values value NNS 10_1101-2021_02_08_430275 128 11 . . . 10_1101-2021_02_08_430275 129 1 ( ( -LRB- 10_1101-2021_02_08_430275 129 2 D d NN 10_1101-2021_02_08_430275 129 3 ) ) -RRB- 10_1101-2021_02_08_430275 129 4 Genomic genomic JJ 10_1101-2021_02_08_430275 129 5 distributions distribution NNS 10_1101-2021_02_08_430275 129 6 of of IN 10_1101-2021_02_08_430275 129 7 ΔAF δaf NN 10_1101-2021_02_08_430275 129 8 values value NNS 10_1101-2021_02_08_430275 129 9 . . . 10_1101-2021_02_08_430275 130 1 Table table NN 10_1101-2021_02_08_430275 130 2 2 2 CD 10_1101-2021_02_08_430275 130 3 . . . 10_1101-2021_02_08_430275 131 1 Chromosomal chromosomal JJ 10_1101-2021_02_08_430275 131 2 distribution distribution NN 10_1101-2021_02_08_430275 131 3 of of IN 10_1101-2021_02_08_430275 131 4 SNPs snp NNS 10_1101-2021_02_08_430275 131 5 - - : 10_1101-2021_02_08_430275 131 6 using use VBG 10_1101-2021_02_08_430275 131 7 only only RB 10_1101-2021_02_08_430275 131 8 the the DT 10_1101-2021_02_08_430275 131 9 bulk bulk JJ 10_1101-2021_02_08_430275 131 10 genome genome NN 10_1101-2021_02_08_430275 131 11 sequences sequence NNS 10_1101-2021_02_08_430275 131 12 Chromosome Chromosome NNP 10_1101-2021_02_08_430275 131 13 sSNPs ssnps CD 10_1101-2021_02_08_430275 131 14 TotalSNPs totalsnps RB 10_1101-2021_02_08_430275 131 15 sSNP ssnp NN 10_1101-2021_02_08_430275 131 16 / / SYM 10_1101-2021_02_08_430275 131 17 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 131 18 1 1 CD 10_1101-2021_02_08_430275 131 19 1335 1335 CD 10_1101-2021_02_08_430275 131 20 163 163 CD 10_1101-2021_02_08_430275 131 21 260 260 CD 10_1101-2021_02_08_430275 131 22 0.0082 0.0082 CD 10_1101-2021_02_08_430275 131 23 2 2 CD 10_1101-2021_02_08_430275 131 24 391 391 CD 10_1101-2021_02_08_430275 131 25 146 146 CD 10_1101-2021_02_08_430275 131 26 877 877 CD 10_1101-2021_02_08_430275 131 27 0.0027 0.0027 CD 10_1101-2021_02_08_430275 131 28 3 3 CD 10_1101-2021_02_08_430275 131 29 578 578 CD 10_1101-2021_02_08_430275 131 30 120 120 CD 10_1101-2021_02_08_430275 131 31 319 319 CD 10_1101-2021_02_08_430275 131 32 0.0048 0.0048 CD 10_1101-2021_02_08_430275 131 33 4 4 CD 10_1101-2021_02_08_430275 131 34 442 442 CD 10_1101-2021_02_08_430275 131 35 110 110 CD 10_1101-2021_02_08_430275 131 36 952 952 CD 10_1101-2021_02_08_430275 131 37 0.0040 0.0040 CD 10_1101-2021_02_08_430275 131 38 5 5 CD 10_1101-2021_02_08_430275 131 39 481 481 CD 10_1101-2021_02_08_430275 131 40 103 103 CD 10_1101-2021_02_08_430275 131 41 362 362 CD 10_1101-2021_02_08_430275 131 42 0.0047 0.0047 CD 10_1101-2021_02_08_430275 131 43 6 6 CD 10_1101-2021_02_08_430275 131 44 1724 1724 CD 10_1101-2021_02_08_430275 131 45 103 103 CD 10_1101-2021_02_08_430275 131 46 416 416 CD 10_1101-2021_02_08_430275 131 47 0.0167 0.0167 CD 10_1101-2021_02_08_430275 131 48 7 7 CD 10_1101-2021_02_08_430275 131 49 459 459 CD 10_1101-2021_02_08_430275 131 50 114 114 CD 10_1101-2021_02_08_430275 131 51 564 564 CD 10_1101-2021_02_08_430275 131 52 0.0040 0.0040 CD 10_1101-2021_02_08_430275 131 53 8 8 CD 10_1101-2021_02_08_430275 131 54 373 373 CD 10_1101-2021_02_08_430275 131 55 103 103 CD 10_1101-2021_02_08_430275 131 56 385 385 CD 10_1101-2021_02_08_430275 131 57 0.0036 0.0036 CD 10_1101-2021_02_08_430275 131 58 9 9 CD 10_1101-2021_02_08_430275 131 59 1410 1410 CD 10_1101-2021_02_08_430275 131 60 82 82 CD 10_1101-2021_02_08_430275 131 61 744 744 CD 10_1101-2021_02_08_430275 131 62 0.0170 0.0170 CD 10_1101-2021_02_08_430275 131 63 10 10 CD 10_1101-2021_02_08_430275 131 64 572 572 CD 10_1101-2021_02_08_430275 131 65 78 78 CD 10_1101-2021_02_08_430275 131 66 206 206 CD 10_1101-2021_02_08_430275 131 67 0.0073 0.0073 CD 10_1101-2021_02_08_430275 131 68 11 11 CD 10_1101-2021_02_08_430275 131 69 3120 3120 CD 10_1101-2021_02_08_430275 131 70 112 112 CD 10_1101-2021_02_08_430275 131 71 719 719 CD 10_1101-2021_02_08_430275 131 72 0.0277 0.0277 CD 10_1101-2021_02_08_430275 131 73 12 12 CD 10_1101-2021_02_08_430275 131 74 281 281 CD 10_1101-2021_02_08_430275 131 75 106 106 CD 10_1101-2021_02_08_430275 131 76 381 381 CD 10_1101-2021_02_08_430275 131 77 0.0026 0.0026 CD 10_1101-2021_02_08_430275 131 78 Genome Genome NNP 10_1101-2021_02_08_430275 131 79 - - HYPH 10_1101-2021_02_08_430275 131 80 wide wide JJ 10_1101-2021_02_08_430275 131 81 11 11 CD 10_1101-2021_02_08_430275 131 82 166 166 CD 10_1101-2021_02_08_430275 131 83 1 1 CD 10_1101-2021_02_08_430275 131 84 346 346 CD 10_1101-2021_02_08_430275 131 85 185 185 CD 10_1101-2021_02_08_430275 131 86 0.0083 0.0083 CD 10_1101-2021_02_08_430275 131 87 AD ad NN 10_1101-2021_02_08_430275 131 88 swapping swapping NN 10_1101-2021_02_08_430275 131 89 was be VBD 10_1101-2021_02_08_430275 131 90 performed perform VBN 10_1101-2021_02_08_430275 131 91 on on IN 10_1101-2021_02_08_430275 131 92 only only RB 10_1101-2021_02_08_430275 131 93 67 67 CD 10_1101-2021_02_08_430275 131 94 396 396 CD 10_1101-2021_02_08_430275 131 95 SNPs snp NNS 10_1101-2021_02_08_430275 131 96 , , , 10_1101-2021_02_08_430275 131 97 6.1 6.1 CD 10_1101-2021_02_08_430275 131 98 % % NN 10_1101-2021_02_08_430275 131 99 of of IN 10_1101-2021_02_08_430275 131 100 311 311 CD 10_1101-2021_02_08_430275 131 101 total total JJ 10_1101-2021_02_08_430275 131 102 SNPs snp NNS 10_1101-2021_02_08_430275 131 103 . . . 10_1101-2021_02_08_430275 132 1 312 312 CD 10_1101-2021_02_08_430275 132 2 Discussion discussion NN 10_1101-2021_02_08_430275 132 3 313 313 CD 10_1101-2021_02_08_430275 132 4 We -PRON- PRP 10_1101-2021_02_08_430275 132 5 tested test VBD 10_1101-2021_02_08_430275 132 6 how how WRB 10_1101-2021_02_08_430275 132 7 parental parental JJ 10_1101-2021_02_08_430275 132 8 genome genome JJ 10_1101-2021_02_08_430275 132 9 sequences sequence NNS 10_1101-2021_02_08_430275 132 10 affected affect VBD 10_1101-2021_02_08_430275 132 11 the the DT 10_1101-2021_02_08_430275 132 12 detec- detec- NN 10_1101-2021_02_08_430275 132 13 314 314 CD 10_1101-2021_02_08_430275 132 14 tion tion NN 10_1101-2021_02_08_430275 132 15 of of IN 10_1101-2021_02_08_430275 132 16 SNP SNP NNP 10_1101-2021_02_08_430275 132 17 - - HYPH 10_1101-2021_02_08_430275 132 18 trait trait NN 10_1101-2021_02_08_430275 132 19 associations association NNS 10_1101-2021_02_08_430275 132 20 via via IN 10_1101-2021_02_08_430275 132 21 BSA BSA NNP 10_1101-2021_02_08_430275 132 22 - - HYPH 10_1101-2021_02_08_430275 132 23 Seq Seq NNP 10_1101-2021_02_08_430275 132 24 using use VBG 10_1101-2021_02_08_430275 132 25 a a DT 10_1101-2021_02_08_430275 132 26 dataset dataset JJ 10_1101-2021_02_08_430275 132 27 315 315 CD 10_1101-2021_02_08_430275 132 28 of of IN 10_1101-2021_02_08_430275 132 29 the the DT 10_1101-2021_02_08_430275 132 30 rice rice NN 10_1101-2021_02_08_430275 132 31 root root NN 10_1101-2021_02_08_430275 132 32 - - HYPH 10_1101-2021_02_08_430275 132 33 knot knot NN 10_1101-2021_02_08_430275 132 34 nematode nematode NNP 10_1101-2021_02_08_430275 132 35 resistance resistance NNP 10_1101-2021_02_08_430275 132 36 . . . 10_1101-2021_02_08_430275 133 1 Using use VBG 10_1101-2021_02_08_430275 133 2 the the DT 10_1101-2021_02_08_430275 133 3 genome genome JJ 10_1101-2021_02_08_430275 133 4 316 316 CD 10_1101-2021_02_08_430275 133 5 sequences sequence NNS 10_1101-2021_02_08_430275 133 6 of of IN 10_1101-2021_02_08_430275 133 7 both both CC 10_1101-2021_02_08_430275 133 8 the the DT 10_1101-2021_02_08_430275 133 9 parents parent NNS 10_1101-2021_02_08_430275 133 10 and and CC 10_1101-2021_02_08_430275 133 11 bulks bulk NNS 10_1101-2021_02_08_430275 133 12 , , , 10_1101-2021_02_08_430275 133 13 a a DT 10_1101-2021_02_08_430275 133 14 major major JJ 10_1101-2021_02_08_430275 133 15 locus locus NN 10_1101-2021_02_08_430275 133 16 on on IN 10_1101-2021_02_08_430275 133 17 317 317 CD 10_1101-2021_02_08_430275 133 18 chromosome chromosome NN 10_1101-2021_02_08_430275 133 19 11 11 CD 10_1101-2021_02_08_430275 133 20 and and CC 10_1101-2021_02_08_430275 133 21 a a DT 10_1101-2021_02_08_430275 133 22 minor minor JJ 10_1101-2021_02_08_430275 133 23 locus locus NN 10_1101-2021_02_08_430275 133 24 on on IN 10_1101-2021_02_08_430275 133 25 chromosome chromosome NN 10_1101-2021_02_08_430275 133 26 9 9 CD 10_1101-2021_02_08_430275 133 27 were be VBD 10_1101-2021_02_08_430275 133 28 de- de- RB 10_1101-2021_02_08_430275 133 29 318 318 CD 10_1101-2021_02_08_430275 133 30 tected tecte VBN 10_1101-2021_02_08_430275 133 31 via via IN 10_1101-2021_02_08_430275 133 32 the the DT 10_1101-2021_02_08_430275 133 33 significant significant JJ 10_1101-2021_02_08_430275 133 34 structural structural JJ 10_1101-2021_02_08_430275 133 35 variant variant JJ 10_1101-2021_02_08_430275 133 36 method method NN 10_1101-2021_02_08_430275 133 37 . . . 10_1101-2021_02_08_430275 134 1 However however RB 10_1101-2021_02_08_430275 134 2 , , , 10_1101-2021_02_08_430275 134 3 319 319 CD 10_1101-2021_02_08_430275 134 4 only only RB 10_1101-2021_02_08_430275 134 5 the the DT 10_1101-2021_02_08_430275 134 6 major major JJ 10_1101-2021_02_08_430275 134 7 locus locus NN 10_1101-2021_02_08_430275 134 8 was be VBD 10_1101-2021_02_08_430275 134 9 detected detect VBN 10_1101-2021_02_08_430275 134 10 via via IN 10_1101-2021_02_08_430275 134 11 the the DT 10_1101-2021_02_08_430275 134 12 G g NN 10_1101-2021_02_08_430275 134 13 - - HYPH 10_1101-2021_02_08_430275 134 14 statistic statistic NN 10_1101-2021_02_08_430275 134 15 method method NN 10_1101-2021_02_08_430275 134 16 320 320 CD 10_1101-2021_02_08_430275 134 17 and and CC 10_1101-2021_02_08_430275 134 18 the the DT 10_1101-2021_02_08_430275 134 19 allele allele NNP 10_1101-2021_02_08_430275 134 20 frequency frequency NN 10_1101-2021_02_08_430275 134 21 method method NN 10_1101-2021_02_08_430275 134 22 . . . 10_1101-2021_02_08_430275 135 1 The the DT 10_1101-2021_02_08_430275 135 2 positions position NNS 10_1101-2021_02_08_430275 135 3 of of IN 10_1101-2021_02_08_430275 135 4 the the DT 10_1101-2021_02_08_430275 135 5 peaks peak NNS 10_1101-2021_02_08_430275 135 6 321 321 CD 10_1101-2021_02_08_430275 135 7 detected detect VBD 10_1101-2021_02_08_430275 135 8 via via IN 10_1101-2021_02_08_430275 135 9 different different JJ 10_1101-2021_02_08_430275 135 10 methods method NNS 10_1101-2021_02_08_430275 135 11 were be VBD 10_1101-2021_02_08_430275 135 12 not not RB 10_1101-2021_02_08_430275 135 13 the the DT 10_1101-2021_02_08_430275 135 14 same same JJ 10_1101-2021_02_08_430275 135 15 , , , 10_1101-2021_02_08_430275 135 16 but but CC 10_1101-2021_02_08_430275 135 17 they -PRON- PRP 10_1101-2021_02_08_430275 135 18 322 322 CD 10_1101-2021_02_08_430275 135 19 were be VBD 10_1101-2021_02_08_430275 135 20 very very RB 10_1101-2021_02_08_430275 135 21 close close JJ 10_1101-2021_02_08_430275 135 22 to to IN 10_1101-2021_02_08_430275 135 23 each each DT 10_1101-2021_02_08_430275 135 24 other other JJ 10_1101-2021_02_08_430275 135 25 . . . 10_1101-2021_02_08_430275 136 1 Using use VBG 10_1101-2021_02_08_430275 136 2 only only RB 10_1101-2021_02_08_430275 136 3 the the DT 10_1101-2021_02_08_430275 136 4 bulk bulk JJ 10_1101-2021_02_08_430275 136 5 genome genome NNP 10_1101-2021_02_08_430275 136 6 323 323 CD 10_1101-2021_02_08_430275 136 7 sequences sequence NNS 10_1101-2021_02_08_430275 136 8 , , , 10_1101-2021_02_08_430275 136 9 the the DT 10_1101-2021_02_08_430275 136 10 major major JJ 10_1101-2021_02_08_430275 136 11 locus locus NN 10_1101-2021_02_08_430275 136 12 can can MD 10_1101-2021_02_08_430275 136 13 be be VB 10_1101-2021_02_08_430275 136 14 detected detect VBN 10_1101-2021_02_08_430275 136 15 via via IN 10_1101-2021_02_08_430275 136 16 only only RB 10_1101-2021_02_08_430275 136 17 the the DT 10_1101-2021_02_08_430275 136 18 signif- signif- JJ 10_1101-2021_02_08_430275 136 19 324 324 CD 10_1101-2021_02_08_430275 136 20 icant icant NN 10_1101-2021_02_08_430275 136 21 structural structural JJ 10_1101-2021_02_08_430275 136 22 variant variant NN 10_1101-2021_02_08_430275 136 23 and and CC 10_1101-2021_02_08_430275 136 24 G g NN 10_1101-2021_02_08_430275 136 25 - - HYPH 10_1101-2021_02_08_430275 136 26 statistic statistic NN 10_1101-2021_02_08_430275 136 27 methods method NNS 10_1101-2021_02_08_430275 136 28 . . . 10_1101-2021_02_08_430275 137 1 The the DT 10_1101-2021_02_08_430275 137 2 allele allele JJ 10_1101-2021_02_08_430275 137 3 325 325 CD 10_1101-2021_02_08_430275 137 4 frequency frequency NN 10_1101-2021_02_08_430275 137 5 method method NN 10_1101-2021_02_08_430275 137 6 uses use VBZ 10_1101-2021_02_08_430275 137 7 the the DT 10_1101-2021_02_08_430275 137 8 ΔAF δaf NN 10_1101-2021_02_08_430275 137 9 value value NN 10_1101-2021_02_08_430275 137 10 of of IN 10_1101-2021_02_08_430275 137 11 a a DT 10_1101-2021_02_08_430275 137 12 SNP SNP NNP 10_1101-2021_02_08_430275 137 13 to to TO 10_1101-2021_02_08_430275 137 14 measure measure VB 10_1101-2021_02_08_430275 137 15 326 326 CD 10_1101-2021_02_08_430275 137 16 allele allele NNS 10_1101-2021_02_08_430275 137 17 ( ( -LRB- 10_1101-2021_02_08_430275 137 18 REF REF NNP 10_1101-2021_02_08_430275 137 19 / / SYM 10_1101-2021_02_08_430275 137 20 ALT ALT NNP 10_1101-2021_02_08_430275 137 21 ) ) -RRB- 10_1101-2021_02_08_430275 137 22 enrichment enrichment NN 10_1101-2021_02_08_430275 137 23 in in IN 10_1101-2021_02_08_430275 137 24 the the DT 10_1101-2021_02_08_430275 137 25 SNP SNP NNP 10_1101-2021_02_08_430275 137 26 locus locus NN 10_1101-2021_02_08_430275 137 27 , , , 10_1101-2021_02_08_430275 137 28 and and CC 10_1101-2021_02_08_430275 137 29 the the DT 10_1101-2021_02_08_430275 137 30 G- G- NNP 10_1101-2021_02_08_430275 137 31 327 327 CD 10_1101-2021_02_08_430275 137 32 statistic statistic NN 10_1101-2021_02_08_430275 137 33 method method NN 10_1101-2021_02_08_430275 137 34 uses use VBZ 10_1101-2021_02_08_430275 137 35 the the DT 10_1101-2021_02_08_430275 137 36 G g NN 10_1101-2021_02_08_430275 137 37 - - HYPH 10_1101-2021_02_08_430275 137 38 statistic statistic NN 10_1101-2021_02_08_430275 137 39 value value NN 10_1101-2021_02_08_430275 137 40 of of IN 10_1101-2021_02_08_430275 137 41 a a DT 10_1101-2021_02_08_430275 137 42 SNP SNP NNP 10_1101-2021_02_08_430275 137 43 to to TO 10_1101-2021_02_08_430275 137 44 measure measure VB 10_1101-2021_02_08_430275 137 45 328 328 CD 10_1101-2021_02_08_430275 137 46 the the DT 10_1101-2021_02_08_430275 137 47 allele allele NNP 10_1101-2021_02_08_430275 137 48 enrichment enrichment NN 10_1101-2021_02_08_430275 137 49 ; ; : 10_1101-2021_02_08_430275 137 50 ΔAF ΔAF NNP 10_1101-2021_02_08_430275 137 51 and and CC 10_1101-2021_02_08_430275 137 52 G G NNP 10_1101-2021_02_08_430275 137 53 - - HYPH 10_1101-2021_02_08_430275 137 54 statistic statistic NN 10_1101-2021_02_08_430275 137 55 are be VBP 10_1101-2021_02_08_430275 137 56 parameters parameter NNS 10_1101-2021_02_08_430275 137 57 329 329 CD 10_1101-2021_02_08_430275 137 58 at at IN 10_1101-2021_02_08_430275 137 59 the the DT 10_1101-2021_02_08_430275 137 60 SNP SNP NNP 10_1101-2021_02_08_430275 137 61 level level NN 10_1101-2021_02_08_430275 137 62 , , , 10_1101-2021_02_08_430275 137 63 therefore therefore RB 10_1101-2021_02_08_430275 137 64 , , , 10_1101-2021_02_08_430275 137 65 both both DT 10_1101-2021_02_08_430275 137 66 methods method NNS 10_1101-2021_02_08_430275 137 67 use use VBP 10_1101-2021_02_08_430275 137 68 a a DT 10_1101-2021_02_08_430275 137 69 SNP SNP NNP 10_1101-2021_02_08_430275 137 70 level level NN 10_1101-2021_02_08_430275 137 71 330 330 CD 10_1101-2021_02_08_430275 137 72 parameter parameter NN 10_1101-2021_02_08_430275 137 73 to to TO 10_1101-2021_02_08_430275 137 74 identify identify VB 10_1101-2021_02_08_430275 137 75 significant significant JJ 10_1101-2021_02_08_430275 137 76 sliding slide VBG 10_1101-2021_02_08_430275 137 77 windows window NNS 10_1101-2021_02_08_430275 137 78 for for IN 10_1101-2021_02_08_430275 137 79 the the DT 10_1101-2021_02_08_430275 137 80 detec- detec- JJ 10_1101-2021_02_08_430275 137 81 331 331 CD 10_1101-2021_02_08_430275 137 82 tion tion NN 10_1101-2021_02_08_430275 137 83 of of IN 10_1101-2021_02_08_430275 137 84 the the DT 10_1101-2021_02_08_430275 137 85 genomic genomic JJ 10_1101-2021_02_08_430275 137 86 region region NN 10_1101-2021_02_08_430275 137 87 - - HYPH 10_1101-2021_02_08_430275 137 88 trait trait NN 10_1101-2021_02_08_430275 137 89 associations association NNS 10_1101-2021_02_08_430275 137 90 . . . 10_1101-2021_02_08_430275 138 1 The the DT 10_1101-2021_02_08_430275 138 2 significant significant JJ 10_1101-2021_02_08_430275 138 3 332 332 CD 10_1101-2021_02_08_430275 138 4 structural structural JJ 10_1101-2021_02_08_430275 138 5 variant variant JJ 10_1101-2021_02_08_430275 138 6 method method NN 10_1101-2021_02_08_430275 138 7 , , , 10_1101-2021_02_08_430275 138 8 however however RB 10_1101-2021_02_08_430275 138 9 , , , 10_1101-2021_02_08_430275 138 10 uses use VBZ 10_1101-2021_02_08_430275 138 11 the the DT 10_1101-2021_02_08_430275 138 12 sSNP ssnp NN 10_1101-2021_02_08_430275 138 13 / / SYM 10_1101-2021_02_08_430275 138 14 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 138 15 333 333 CD 10_1101-2021_02_08_430275 138 16 ratio ratio NN 10_1101-2021_02_08_430275 138 17 , , , 10_1101-2021_02_08_430275 138 18 a a DT 10_1101-2021_02_08_430275 138 19 parameter parameter NN 10_1101-2021_02_08_430275 138 20 at at IN 10_1101-2021_02_08_430275 138 21 the the DT 10_1101-2021_02_08_430275 138 22 sliding slide VBG 10_1101-2021_02_08_430275 138 23 window window NN 10_1101-2021_02_08_430275 138 24 level level NN 10_1101-2021_02_08_430275 138 25 , , , 10_1101-2021_02_08_430275 138 26 to to TO 10_1101-2021_02_08_430275 138 27 measure measure VB 10_1101-2021_02_08_430275 138 28 the the DT 10_1101-2021_02_08_430275 138 29 334 334 CD 10_1101-2021_02_08_430275 138 30 sSNP ssnp NN 10_1101-2021_02_08_430275 138 31 enrichment enrichment NN 10_1101-2021_02_08_430275 138 32 in in IN 10_1101-2021_02_08_430275 138 33 a a DT 10_1101-2021_02_08_430275 138 34 sliding slide VBG 10_1101-2021_02_08_430275 138 35 window window NN 10_1101-2021_02_08_430275 138 36 for for IN 10_1101-2021_02_08_430275 138 37 the the DT 10_1101-2021_02_08_430275 138 38 identification identification NN 10_1101-2021_02_08_430275 138 39 of of IN 10_1101-2021_02_08_430275 138 40 335 335 CD 10_1101-2021_02_08_430275 138 41 the the DT 10_1101-2021_02_08_430275 138 42 trait trait NN 10_1101-2021_02_08_430275 138 43 - - HYPH 10_1101-2021_02_08_430275 138 44 associated associate VBN 10_1101-2021_02_08_430275 138 45 genomic genomic JJ 10_1101-2021_02_08_430275 138 46 regions region NNS 10_1101-2021_02_08_430275 138 47 . . . 10_1101-2021_02_08_430275 139 1 A a DT 10_1101-2021_02_08_430275 139 2 SNP SNP NNP 10_1101-2021_02_08_430275 139 3 normally normally RB 10_1101-2021_02_08_430275 139 4 has have VBZ 10_1101-2021_02_08_430275 139 5 less less JJR 10_1101-2021_02_08_430275 139 6 336 336 CD 10_1101-2021_02_08_430275 139 7 than than IN 10_1101-2021_02_08_430275 139 8 100 100 CD 10_1101-2021_02_08_430275 139 9 reads read NNS 10_1101-2021_02_08_430275 139 10 because because IN 10_1101-2021_02_08_430275 139 11 of of IN 10_1101-2021_02_08_430275 139 12 the the DT 10_1101-2021_02_08_430275 139 13 cost cost NN 10_1101-2021_02_08_430275 139 14 concern concern NN 10_1101-2021_02_08_430275 139 15 , , , 10_1101-2021_02_08_430275 139 16 while while IN 10_1101-2021_02_08_430275 139 17 a a DT 10_1101-2021_02_08_430275 139 18 sliding slide VBG 10_1101-2021_02_08_430275 139 19 337 337 CD 10_1101-2021_02_08_430275 139 20 4 4 CD 10_1101-2021_02_08_430275 139 21 | | CD 10_1101-2021_02_08_430275 139 22 https://doi.org/10.1101/654137 https://doi.org/10.1101/654137 NN 10_1101-2021_02_08_430275 139 23 Zhang Zhang NNP 10_1101-2021_02_08_430275 139 24 et et NNP 10_1101-2021_02_08_430275 139 25 al al NNP 10_1101-2021_02_08_430275 139 26 . . . 10_1101-2021_02_08_430275 140 1 .CC .CC NFP 10_1101-2021_02_08_430275 140 2 - - : 10_1101-2021_02_08_430275 140 3 BY by IN 10_1101-2021_02_08_430275 140 4 - - HYPH 10_1101-2021_02_08_430275 140 5 NC NC NNP 10_1101-2021_02_08_430275 140 6 - - HYPH 10_1101-2021_02_08_430275 140 7 ND ND NNP 10_1101-2021_02_08_430275 140 8 4.0 4.0 CD 10_1101-2021_02_08_430275 140 9 International International NNP 10_1101-2021_02_08_430275 140 10 licenseavailable licenseavailable NN 10_1101-2021_02_08_430275 140 11 under under IN 10_1101-2021_02_08_430275 140 12 a a DT 10_1101-2021_02_08_430275 140 13 ( ( -LRB- 10_1101-2021_02_08_430275 140 14 which which WDT 10_1101-2021_02_08_430275 140 15 was be VBD 10_1101-2021_02_08_430275 140 16 not not RB 10_1101-2021_02_08_430275 140 17 certified certify VBN 10_1101-2021_02_08_430275 140 18 by by IN 10_1101-2021_02_08_430275 140 19 peer peer NN 10_1101-2021_02_08_430275 140 20 review review NN 10_1101-2021_02_08_430275 140 21 ) ) -RRB- 10_1101-2021_02_08_430275 140 22 is be VBZ 10_1101-2021_02_08_430275 140 23 the the DT 10_1101-2021_02_08_430275 140 24 author author NN 10_1101-2021_02_08_430275 140 25 / / SYM 10_1101-2021_02_08_430275 140 26 funder funder NN 10_1101-2021_02_08_430275 140 27 , , , 10_1101-2021_02_08_430275 140 28 who who WP 10_1101-2021_02_08_430275 140 29 has have VBZ 10_1101-2021_02_08_430275 140 30 granted grant VBN 10_1101-2021_02_08_430275 140 31 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 140 32 a a DT 10_1101-2021_02_08_430275 140 33 license license NN 10_1101-2021_02_08_430275 140 34 to to TO 10_1101-2021_02_08_430275 140 35 display display VB 10_1101-2021_02_08_430275 140 36 the the DT 10_1101-2021_02_08_430275 140 37 preprint preprint NN 10_1101-2021_02_08_430275 140 38 in in IN 10_1101-2021_02_08_430275 140 39 perpetuity perpetuity NN 10_1101-2021_02_08_430275 140 40 . . . 10_1101-2021_02_08_430275 141 1 It -PRON- PRP 10_1101-2021_02_08_430275 141 2 is be VBZ 10_1101-2021_02_08_430275 141 3 made make VBN 10_1101-2021_02_08_430275 141 4 The the DT 10_1101-2021_02_08_430275 141 5 copyright copyright NN 10_1101-2021_02_08_430275 141 6 holder holder NN 10_1101-2021_02_08_430275 141 7 for for IN 10_1101-2021_02_08_430275 141 8 this this DT 10_1101-2021_02_08_430275 141 9 preprintthis preprintthis NN 10_1101-2021_02_08_430275 141 10 version version NN 10_1101-2021_02_08_430275 141 11 posted post VBD 10_1101-2021_02_08_430275 141 12 February February NNP 10_1101-2021_02_08_430275 141 13 10 10 CD 10_1101-2021_02_08_430275 141 14 , , , 10_1101-2021_02_08_430275 141 15 2021 2021 CD 10_1101-2021_02_08_430275 141 16 . . . 10_1101-2021_02_08_430275 141 17 ; ; : 10_1101-2021_02_08_430275 141 18 https://doi.org/10.1101/2021.02.08.430275doi https://doi.org/10.1101/2021.02.08.430275doi ADD 10_1101-2021_02_08_430275 141 19 : : : 10_1101-2021_02_08_430275 141 20 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 141 21 preprint preprint NN 10_1101-2021_02_08_430275 141 22 https://doi.org/10.1101/654137 https://doi.org/10.1101/654137 VBZ 10_1101-2021_02_08_430275 141 23 https://doi.org/10.1101/2021.02.08.430275 https://doi.org/10.1101/2021.02.08.430275 NNP 10_1101-2021_02_08_430275 141 24 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_02_08_430275 141 25 DR DR NNP 10_1101-2021_02_08_430275 141 26 AF AF NNP 10_1101-2021_02_08_430275 141 27 T T NNP 10_1101-2021_02_08_430275 141 28 0 0 CD 10_1101-2021_02_08_430275 141 29 2500 2500 CD 10_1101-2021_02_08_430275 141 30 5000 5000 CD 10_1101-2021_02_08_430275 141 31 7500 7500 CD 10_1101-2021_02_08_430275 141 32 10000 10000 CD 10_1101-2021_02_08_430275 141 33 12500 12500 CD 10_1101-2021_02_08_430275 141 34 N N NNP 10_1101-2021_02_08_430275 141 35 um um UH 10_1101-2021_02_08_430275 141 36 be be VB 10_1101-2021_02_08_430275 141 37 r r NN 10_1101-2021_02_08_430275 141 38 o o NN 10_1101-2021_02_08_430275 141 39 f f NN 10_1101-2021_02_08_430275 141 40 S s NN 10_1101-2021_02_08_430275 141 41 N N NNP 10_1101-2021_02_08_430275 141 42 P P NNP 10_1101-2021_02_08_430275 141 43 s s NN 10_1101-2021_02_08_430275 141 44 Chr1 Chr1 NNPS 10_1101-2021_02_08_430275 141 45 Chr2 Chr2 NNP 10_1101-2021_02_08_430275 141 46 Chr3 chr3 NN 10_1101-2021_02_08_430275 141 47 Chr4 chr4 NN 10_1101-2021_02_08_430275 141 48 Chr5 Chr5 VBZ 10_1101-2021_02_08_430275 141 49 Chr6 Chr6 NNP 10_1101-2021_02_08_430275 141 50 Chr7 Chr7 NNP 10_1101-2021_02_08_430275 141 51 Chr8 Chr8 NNP 10_1101-2021_02_08_430275 141 52 Chr9 Chr9 NNP 10_1101-2021_02_08_430275 141 53 Chr10 Chr10 NNP 10_1101-2021_02_08_430275 141 54 Chr11 Chr11 NNP 10_1101-2021_02_08_430275 141 55 Chr12 Chr12 NNP 10_1101-2021_02_08_430275 141 56 0.0 0.0 CD 10_1101-2021_02_08_430275 141 57 0.2 0.2 CD 10_1101-2021_02_08_430275 141 58 0.4 0.4 CD 10_1101-2021_02_08_430275 141 59 sS ss NN 10_1101-2021_02_08_430275 141 60 N N NNP 10_1101-2021_02_08_430275 141 61 P P NNP 10_1101-2021_02_08_430275 141 62 /to /to : 10_1101-2021_02_08_430275 141 63 ta ta ADD 10_1101-2021_02_08_430275 141 64 lS lS NNP 10_1101-2021_02_08_430275 141 65 N N NNP 10_1101-2021_02_08_430275 141 66 P P NNP 10_1101-2021_02_08_430275 141 67 2.5 2.5 CD 10_1101-2021_02_08_430275 141 68 5.0 5.0 CD 10_1101-2021_02_08_430275 141 69 7.5 7.5 CD 10_1101-2021_02_08_430275 141 70 G g NN 10_1101-2021_02_08_430275 141 71 -s -s : 10_1101-2021_02_08_430275 141 72 ta ta ADD 10_1101-2021_02_08_430275 141 73 tis tis NNP 10_1101-2021_02_08_430275 141 74 tic tic NNP 10_1101-2021_02_08_430275 141 75 0 0 CD 10_1101-2021_02_08_430275 141 76 1 1 CD 10_1101-2021_02_08_430275 141 77 2 2 CD 10_1101-2021_02_08_430275 141 78 3 3 CD 10_1101-2021_02_08_430275 141 79 4 4 CD 10_1101-2021_02_08_430275 141 80 0.5 0.5 CD 10_1101-2021_02_08_430275 141 81 0.0 0.0 CD 10_1101-2021_02_08_430275 141 82 0.5 0.5 CD 10_1101-2021_02_08_430275 141 83 A a NN 10_1101-2021_02_08_430275 141 84 F f NN 10_1101-2021_02_08_430275 141 85 0 0 CD 10_1101-2021_02_08_430275 141 86 1 1 CD 10_1101-2021_02_08_430275 141 87 2 2 CD 10_1101-2021_02_08_430275 141 88 3 3 CD 10_1101-2021_02_08_430275 141 89 0 0 CD 10_1101-2021_02_08_430275 141 90 1 1 CD 10_1101-2021_02_08_430275 141 91 2 2 CD 10_1101-2021_02_08_430275 141 92 3 3 CD 10_1101-2021_02_08_430275 141 93 0 0 CD 10_1101-2021_02_08_430275 141 94 1 1 CD 10_1101-2021_02_08_430275 141 95 2 2 CD 10_1101-2021_02_08_430275 141 96 3 3 CD 10_1101-2021_02_08_430275 141 97 0 0 CD 10_1101-2021_02_08_430275 141 98 1 1 CD 10_1101-2021_02_08_430275 141 99 2 2 CD 10_1101-2021_02_08_430275 141 100 0 0 CD 10_1101-2021_02_08_430275 141 101 1 1 CD 10_1101-2021_02_08_430275 141 102 2 2 CD 10_1101-2021_02_08_430275 141 103 0 0 CD 10_1101-2021_02_08_430275 141 104 1 1 CD 10_1101-2021_02_08_430275 141 105 2 2 CD 10_1101-2021_02_08_430275 141 106 0 0 CD 10_1101-2021_02_08_430275 141 107 1 1 CD 10_1101-2021_02_08_430275 141 108 2 2 CD 10_1101-2021_02_08_430275 141 109 0 0 CD 10_1101-2021_02_08_430275 141 110 1 1 CD 10_1101-2021_02_08_430275 141 111 2 2 CD 10_1101-2021_02_08_430275 141 112 0 0 CD 10_1101-2021_02_08_430275 141 113 1 1 CD 10_1101-2021_02_08_430275 141 114 2 2 CD 10_1101-2021_02_08_430275 141 115 0 0 CD 10_1101-2021_02_08_430275 141 116 1 1 CD 10_1101-2021_02_08_430275 141 117 2 2 CD 10_1101-2021_02_08_430275 141 118 0 0 CD 10_1101-2021_02_08_430275 141 119 1 1 CD 10_1101-2021_02_08_430275 141 120 2 2 CD 10_1101-2021_02_08_430275 141 121 Genomic genomic JJ 10_1101-2021_02_08_430275 141 122 position position NN 10_1101-2021_02_08_430275 141 123 ( ( -LRB- 10_1101-2021_02_08_430275 141 124 ×10 ×10 NFP 10_1101-2021_02_08_430275 141 125 Mb mb NN 10_1101-2021_02_08_430275 141 126 ) ) -RRB- 10_1101-2021_02_08_430275 141 127 A A NNP 10_1101-2021_02_08_430275 141 128 B b NN 10_1101-2021_02_08_430275 141 129 C c NN 10_1101-2021_02_08_430275 141 130 D d NN 10_1101-2021_02_08_430275 141 131 Figure figure NN 10_1101-2021_02_08_430275 141 132 2 2 CD 10_1101-2021_02_08_430275 141 133 . . . 10_1101-2021_02_08_430275 142 1 BSA BSA NNP 10_1101-2021_02_08_430275 142 2 - - HYPH 10_1101-2021_02_08_430275 142 3 Seq seq NN 10_1101-2021_02_08_430275 142 4 data data NN 10_1101-2021_02_08_430275 142 5 analysis analysis NN 10_1101-2021_02_08_430275 142 6 using use VBG 10_1101-2021_02_08_430275 142 7 only only RB 10_1101-2021_02_08_430275 142 8 the the DT 10_1101-2021_02_08_430275 142 9 bulk bulk JJ 10_1101-2021_02_08_430275 142 10 genome genome JJ 10_1101-2021_02_08_430275 142 11 sequences sequence NNS 10_1101-2021_02_08_430275 142 12 . . . 10_1101-2021_02_08_430275 143 1 The the DT 10_1101-2021_02_08_430275 143 2 red red JJ 10_1101-2021_02_08_430275 143 3 lines line NNS 10_1101-2021_02_08_430275 143 4 / / SYM 10_1101-2021_02_08_430275 143 5 curves curve NNS 10_1101-2021_02_08_430275 143 6 are be VBP 10_1101-2021_02_08_430275 143 7 the the DT 10_1101-2021_02_08_430275 143 8 thresholds threshold NNS 10_1101-2021_02_08_430275 143 9 . . . 10_1101-2021_02_08_430275 144 1 ( ( -LRB- 10_1101-2021_02_08_430275 144 2 A a DT 10_1101-2021_02_08_430275 144 3 ) ) -RRB- 10_1101-2021_02_08_430275 144 4 Genomic genomic JJ 10_1101-2021_02_08_430275 144 5 distributions distribution NNS 10_1101-2021_02_08_430275 144 6 of of IN 10_1101-2021_02_08_430275 144 7 sSNPs ssnps CD 10_1101-2021_02_08_430275 144 8 ( ( -LRB- 10_1101-2021_02_08_430275 144 9 blue blue NNP 10_1101-2021_02_08_430275 144 10 ) ) -RRB- 10_1101-2021_02_08_430275 144 11 and and CC 10_1101-2021_02_08_430275 144 12 totalSNPs totalsnps RB 10_1101-2021_02_08_430275 144 13 ( ( -LRB- 10_1101-2021_02_08_430275 144 14 black black JJ 10_1101-2021_02_08_430275 144 15 ) ) -RRB- 10_1101-2021_02_08_430275 144 16 . . . 10_1101-2021_02_08_430275 145 1 ( ( -LRB- 10_1101-2021_02_08_430275 145 2 B b NN 10_1101-2021_02_08_430275 145 3 ) ) -RRB- 10_1101-2021_02_08_430275 145 4 Genomic genomic JJ 10_1101-2021_02_08_430275 145 5 distributions distribution NNS 10_1101-2021_02_08_430275 145 6 of of IN 10_1101-2021_02_08_430275 145 7 sSNP ssnp NN 10_1101-2021_02_08_430275 145 8 / / SYM 10_1101-2021_02_08_430275 145 9 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 145 10 ratios ratio NNS 10_1101-2021_02_08_430275 145 11 . . . 10_1101-2021_02_08_430275 146 1 ( ( -LRB- 10_1101-2021_02_08_430275 146 2 C C NNP 10_1101-2021_02_08_430275 146 3 ) ) -RRB- 10_1101-2021_02_08_430275 146 4 Genomic genomic JJ 10_1101-2021_02_08_430275 146 5 distributions distribution NNS 10_1101-2021_02_08_430275 146 6 of of IN 10_1101-2021_02_08_430275 146 7 G g NN 10_1101-2021_02_08_430275 146 8 - - HYPH 10_1101-2021_02_08_430275 146 9 statistic statistic NN 10_1101-2021_02_08_430275 146 10 values value NNS 10_1101-2021_02_08_430275 146 11 . . . 10_1101-2021_02_08_430275 147 1 ( ( -LRB- 10_1101-2021_02_08_430275 147 2 D d NN 10_1101-2021_02_08_430275 147 3 ) ) -RRB- 10_1101-2021_02_08_430275 147 4 Genomic genomic JJ 10_1101-2021_02_08_430275 147 5 distributions distribution NNS 10_1101-2021_02_08_430275 147 6 of of IN 10_1101-2021_02_08_430275 147 7 ΔAF δaf NN 10_1101-2021_02_08_430275 147 8 values value NNS 10_1101-2021_02_08_430275 147 9 . . . 10_1101-2021_02_08_430275 148 1 window window NN 10_1101-2021_02_08_430275 148 2 normally normally RB 10_1101-2021_02_08_430275 148 3 contains contain VBZ 10_1101-2021_02_08_430275 148 4 thousands thousand NNS 10_1101-2021_02_08_430275 148 5 of of IN 10_1101-2021_02_08_430275 148 6 SNPs snp NNS 10_1101-2021_02_08_430275 148 7 . . . 10_1101-2021_02_08_430275 149 1 Thus thus RB 10_1101-2021_02_08_430275 149 2 , , , 10_1101-2021_02_08_430275 149 3 the the DT 10_1101-2021_02_08_430275 149 4 sig-338 sig-338 JJ 10_1101-2021_02_08_430275 149 5 nificant nificant JJ 10_1101-2021_02_08_430275 149 6 structural structural JJ 10_1101-2021_02_08_430275 149 7 variant variant JJ 10_1101-2021_02_08_430275 149 8 method method NN 10_1101-2021_02_08_430275 149 9 has have VBZ 10_1101-2021_02_08_430275 149 10 much much RB 10_1101-2021_02_08_430275 149 11 higher high JJR 10_1101-2021_02_08_430275 149 12 statistical339 statistical339 NNP 10_1101-2021_02_08_430275 149 13 power power NN 10_1101-2021_02_08_430275 149 14 , , , 10_1101-2021_02_08_430275 149 15 which which WDT 10_1101-2021_02_08_430275 149 16 is be VBZ 10_1101-2021_02_08_430275 149 17 consistent consistent JJ 10_1101-2021_02_08_430275 149 18 with with IN 10_1101-2021_02_08_430275 149 19 our -PRON- PRP$ 10_1101-2021_02_08_430275 149 20 observation observation NN 10_1101-2021_02_08_430275 149 21 . . . 10_1101-2021_02_08_430275 150 1 Our -PRON- PRP$ 10_1101-2021_02_08_430275 150 2 results340 results340 NNP 10_1101-2021_02_08_430275 150 3 revealed reveal VBD 10_1101-2021_02_08_430275 150 4 that that IN 10_1101-2021_02_08_430275 150 5 the the DT 10_1101-2021_02_08_430275 150 6 parental parental JJ 10_1101-2021_02_08_430275 150 7 genome genome NN 10_1101-2021_02_08_430275 150 8 sequences sequence NNS 10_1101-2021_02_08_430275 150 9 did do VBD 10_1101-2021_02_08_430275 150 10 not not RB 10_1101-2021_02_08_430275 150 11 much341 much341 RB 10_1101-2021_02_08_430275 150 12 affect affect VB 10_1101-2021_02_08_430275 150 13 the the DT 10_1101-2021_02_08_430275 150 14 plot plot NN 10_1101-2021_02_08_430275 150 15 patterns pattern NNS 10_1101-2021_02_08_430275 150 16 of of IN 10_1101-2021_02_08_430275 150 17 the the DT 10_1101-2021_02_08_430275 150 18 sSNP ssnp NN 10_1101-2021_02_08_430275 150 19 / / SYM 10_1101-2021_02_08_430275 150 20 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 150 21 ratios ratio NNS 10_1101-2021_02_08_430275 150 22 and and CC 10_1101-2021_02_08_430275 150 23 the342 the342 NNP 10_1101-2021_02_08_430275 150 24 G g NN 10_1101-2021_02_08_430275 150 25 - - HYPH 10_1101-2021_02_08_430275 150 26 statistic statistic NN 10_1101-2021_02_08_430275 150 27 values value NNS 10_1101-2021_02_08_430275 150 28 . . . 10_1101-2021_02_08_430275 151 1 However however RB 10_1101-2021_02_08_430275 151 2 , , , 10_1101-2021_02_08_430275 151 3 the the DT 10_1101-2021_02_08_430275 151 4 plot plot NN 10_1101-2021_02_08_430275 151 5 patterns pattern NNS 10_1101-2021_02_08_430275 151 6 of of IN 10_1101-2021_02_08_430275 151 7 the the DT 10_1101-2021_02_08_430275 151 8 ΔAF343 ΔAF343 NNP 10_1101-2021_02_08_430275 151 9 value value NN 10_1101-2021_02_08_430275 151 10 of of IN 10_1101-2021_02_08_430275 151 11 chromosome chromosome NN 10_1101-2021_02_08_430275 151 12 11 11 CD 10_1101-2021_02_08_430275 151 13 were be VBD 10_1101-2021_02_08_430275 151 14 altered alter VBN 10_1101-2021_02_08_430275 151 15 dramatically dramatically RB 10_1101-2021_02_08_430275 151 16 when when WRB 10_1101-2021_02_08_430275 151 17 the344 the344 , 10_1101-2021_02_08_430275 151 18 parental parental JJ 10_1101-2021_02_08_430275 151 19 genome genome NN 10_1101-2021_02_08_430275 151 20 sequences sequence NNS 10_1101-2021_02_08_430275 151 21 were be VBD 10_1101-2021_02_08_430275 151 22 not not RB 10_1101-2021_02_08_430275 151 23 used.345 used.345 NNP 10_1101-2021_02_08_430275 151 24 The the DT 10_1101-2021_02_08_430275 151 25 significant significant JJ 10_1101-2021_02_08_430275 151 26 structural structural JJ 10_1101-2021_02_08_430275 151 27 variant variant JJ 10_1101-2021_02_08_430275 151 28 method method NN 10_1101-2021_02_08_430275 151 29 assesses assess VBZ 10_1101-2021_02_08_430275 151 30 if if IN 10_1101-2021_02_08_430275 151 31 a a DT 10_1101-2021_02_08_430275 151 32 SNP346 SNP346 NNP 10_1101-2021_02_08_430275 151 33 is be VBZ 10_1101-2021_02_08_430275 151 34 likely likely RB 10_1101-2021_02_08_430275 151 35 associated associate VBN 10_1101-2021_02_08_430275 151 36 with with IN 10_1101-2021_02_08_430275 151 37 the the DT 10_1101-2021_02_08_430275 151 38 trait trait NN 10_1101-2021_02_08_430275 151 39 via via IN 10_1101-2021_02_08_430275 151 40 Fisher Fisher NNP 10_1101-2021_02_08_430275 151 41 ’s ’s POS 10_1101-2021_02_08_430275 151 42 exact exact JJ 10_1101-2021_02_08_430275 151 43 test test NN 10_1101-2021_02_08_430275 151 44 . . . 10_1101-2021_02_08_430275 152 1 The347 the347 LS 10_1101-2021_02_08_430275 152 2 greater great JJR 10_1101-2021_02_08_430275 152 3 the the DT 10_1101-2021_02_08_430275 152 4 ALT ALT NNP 10_1101-2021_02_08_430275 152 5 proportion proportion NN 10_1101-2021_02_08_430275 152 6 differences difference NNS 10_1101-2021_02_08_430275 152 7 between between IN 10_1101-2021_02_08_430275 152 8 the the DT 10_1101-2021_02_08_430275 152 9 bulks bulk NNS 10_1101-2021_02_08_430275 152 10 , , , 10_1101-2021_02_08_430275 152 11 the348 the348 , 10_1101-2021_02_08_430275 152 12 less less JJR 10_1101-2021_02_08_430275 152 13 the the DT 10_1101-2021_02_08_430275 152 14 P p NN 10_1101-2021_02_08_430275 152 15 - - HYPH 10_1101-2021_02_08_430275 152 16 value value NN 10_1101-2021_02_08_430275 152 17 of of IN 10_1101-2021_02_08_430275 152 18 the the DT 10_1101-2021_02_08_430275 152 19 Fisher Fisher NNP 10_1101-2021_02_08_430275 152 20 ’s ’s POS 10_1101-2021_02_08_430275 152 21 exact exact JJ 10_1101-2021_02_08_430275 152 22 test test NN 10_1101-2021_02_08_430275 152 23 , , , 10_1101-2021_02_08_430275 152 24 and and CC 10_1101-2021_02_08_430275 152 25 the the DT 10_1101-2021_02_08_430275 152 26 more more RBR 10_1101-2021_02_08_430275 152 27 likely349 likely349 NNP 10_1101-2021_02_08_430275 152 28 the the DT 10_1101-2021_02_08_430275 152 29 SNP SNP NNP 10_1101-2021_02_08_430275 152 30 is be VBZ 10_1101-2021_02_08_430275 152 31 associated associate VBN 10_1101-2021_02_08_430275 152 32 with with IN 10_1101-2021_02_08_430275 152 33 the the DT 10_1101-2021_02_08_430275 152 34 trait trait NN 10_1101-2021_02_08_430275 152 35 . . . 10_1101-2021_02_08_430275 153 1 Fisher Fisher NNP 10_1101-2021_02_08_430275 153 2 ’s ’s POS 10_1101-2021_02_08_430275 153 3 exact exact JJ 10_1101-2021_02_08_430275 153 4 test test NN 10_1101-2021_02_08_430275 153 5 takes take VBZ 10_1101-2021_02_08_430275 153 6 a350 a350 JJ 10_1101-2021_02_08_430275 153 7 numpy numpy JJ 10_1101-2021_02_08_430275 153 8 array array NN 10_1101-2021_02_08_430275 153 9 or or CC 10_1101-2021_02_08_430275 153 10 a a DT 10_1101-2021_02_08_430275 153 11 Python Python NNP 10_1101-2021_02_08_430275 153 12 list list NN 10_1101-2021_02_08_430275 153 13 as as IN 10_1101-2021_02_08_430275 153 14 its -PRON- PRP$ 10_1101-2021_02_08_430275 153 15 input input NN 10_1101-2021_02_08_430275 153 16 , , , 10_1101-2021_02_08_430275 153 17 the the DT 10_1101-2021_02_08_430275 153 18 same same JJ 10_1101-2021_02_08_430275 153 19 P p NN 10_1101-2021_02_08_430275 153 20 - - HYPH 10_1101-2021_02_08_430275 153 21 value value NN 10_1101-2021_02_08_430275 153 22 will351 will351 NN 10_1101-2021_02_08_430275 153 23 be be VB 10_1101-2021_02_08_430275 153 24 obtained obtain VBN 10_1101-2021_02_08_430275 153 25 with with IN 10_1101-2021_02_08_430275 153 26 either either DT 10_1101-2021_02_08_430275 153 27 [ [ -LRB- 10_1101-2021_02_08_430275 153 28 [ [ -LRB- 10_1101-2021_02_08_430275 153 29 ADref1 ADref1 NNP 10_1101-2021_02_08_430275 153 30 , , , 10_1101-2021_02_08_430275 153 31 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 153 32 ] ] -RRB- 10_1101-2021_02_08_430275 153 33 , , , 10_1101-2021_02_08_430275 153 34 [ [ -LRB- 10_1101-2021_02_08_430275 153 35 ADref2 ADref2 NNP 10_1101-2021_02_08_430275 153 36 , , , 10_1101-2021_02_08_430275 153 37 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 153 38 ] ] -RRB- 10_1101-2021_02_08_430275 153 39 ] ] -RRB- 10_1101-2021_02_08_430275 153 40 or352 or352 NN 10_1101-2021_02_08_430275 153 41 [ [ -LRB- 10_1101-2021_02_08_430275 153 42 [ [ -LRB- 10_1101-2021_02_08_430275 153 43 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 153 44 , , , 10_1101-2021_02_08_430275 153 45 ADref1 ADref1 NNP 10_1101-2021_02_08_430275 153 46 ] ] -RRB- 10_1101-2021_02_08_430275 153 47 , , , 10_1101-2021_02_08_430275 153 48 [ [ -LRB- 10_1101-2021_02_08_430275 153 49 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 153 50 , , , 10_1101-2021_02_08_430275 153 51 ADref2 ADref2 NNP 10_1101-2021_02_08_430275 153 52 ] ] -RRB- 10_1101-2021_02_08_430275 153 53 ] ] -RRB- 10_1101-2021_02_08_430275 153 54 as as IN 10_1101-2021_02_08_430275 153 55 its -PRON- PRP$ 10_1101-2021_02_08_430275 153 56 input input NN 10_1101-2021_02_08_430275 153 57 . . . 10_1101-2021_02_08_430275 154 1 The the DT 10_1101-2021_02_08_430275 154 2 G G NNP 10_1101-2021_02_08_430275 154 3 - - HYPH 10_1101-2021_02_08_430275 154 4 statistic353 statistic353 NNP 10_1101-2021_02_08_430275 154 5 method method NN 10_1101-2021_02_08_430275 154 6 assesses assess VBZ 10_1101-2021_02_08_430275 154 7 if if IN 10_1101-2021_02_08_430275 154 8 a a DT 10_1101-2021_02_08_430275 154 9 SNP SNP NNP 10_1101-2021_02_08_430275 154 10 is be VBZ 10_1101-2021_02_08_430275 154 11 likely likely RB 10_1101-2021_02_08_430275 154 12 associated associate VBN 10_1101-2021_02_08_430275 154 13 with with IN 10_1101-2021_02_08_430275 154 14 the the DT 10_1101-2021_02_08_430275 154 15 trait354 trait354 NN 10_1101-2021_02_08_430275 154 16 via via IN 10_1101-2021_02_08_430275 154 17 the the DT 10_1101-2021_02_08_430275 154 18 G g NN 10_1101-2021_02_08_430275 154 19 - - HYPH 10_1101-2021_02_08_430275 154 20 test test NN 10_1101-2021_02_08_430275 154 21 ; ; : 10_1101-2021_02_08_430275 154 22 the the DT 10_1101-2021_02_08_430275 154 23 greater great JJR 10_1101-2021_02_08_430275 154 24 the the DT 10_1101-2021_02_08_430275 154 25 G g NN 10_1101-2021_02_08_430275 154 26 - - HYPH 10_1101-2021_02_08_430275 154 27 statistic statistic NN 10_1101-2021_02_08_430275 154 28 value value NN 10_1101-2021_02_08_430275 154 29 of of IN 10_1101-2021_02_08_430275 154 30 a a DT 10_1101-2021_02_08_430275 154 31 SNP SNP NNP 10_1101-2021_02_08_430275 154 32 , , , 10_1101-2021_02_08_430275 154 33 the355 the355 PRP 10_1101-2021_02_08_430275 154 34 more more RBR 10_1101-2021_02_08_430275 154 35 likely likely JJ 10_1101-2021_02_08_430275 154 36 it -PRON- PRP 10_1101-2021_02_08_430275 154 37 contributes contribute VBZ 10_1101-2021_02_08_430275 154 38 to to IN 10_1101-2021_02_08_430275 154 39 the the DT 10_1101-2021_02_08_430275 154 40 trait trait NN 10_1101-2021_02_08_430275 154 41 phenotype phenotype NN 10_1101-2021_02_08_430275 154 42 ( ( -LRB- 10_1101-2021_02_08_430275 154 43 11 11 CD 10_1101-2021_02_08_430275 154 44 ) ) -RRB- 10_1101-2021_02_08_430275 154 45 . . . 10_1101-2021_02_08_430275 155 1 The the DT 10_1101-2021_02_08_430275 155 2 G-356 G-356 NNP 10_1101-2021_02_08_430275 155 3 statistic statistic NN 10_1101-2021_02_08_430275 155 4 values value NNS 10_1101-2021_02_08_430275 155 5 are be VBP 10_1101-2021_02_08_430275 155 6 the the DT 10_1101-2021_02_08_430275 155 7 same same JJ 10_1101-2021_02_08_430275 155 8 with with IN 10_1101-2021_02_08_430275 155 9 either either DT 10_1101-2021_02_08_430275 155 10 input input NN 10_1101-2021_02_08_430275 155 11 [ [ -LRB- 10_1101-2021_02_08_430275 155 12 [ [ -LRB- 10_1101-2021_02_08_430275 155 13 ADref1 ADref1 NNP 10_1101-2021_02_08_430275 155 14 , , , 10_1101-2021_02_08_430275 155 15 ADalt1],357 ADalt1],357 NNP 10_1101-2021_02_08_430275 155 16 [ [ -LRB- 10_1101-2021_02_08_430275 155 17 ADref2 ADref2 NNP 10_1101-2021_02_08_430275 155 18 , , , 10_1101-2021_02_08_430275 155 19 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 155 20 ] ] -RRB- 10_1101-2021_02_08_430275 155 21 ] ] -RRB- 10_1101-2021_02_08_430275 155 22 or or CC 10_1101-2021_02_08_430275 155 23 [ [ -LRB- 10_1101-2021_02_08_430275 155 24 [ [ -LRB- 10_1101-2021_02_08_430275 155 25 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 155 26 , , , 10_1101-2021_02_08_430275 155 27 ADref1 ADref1 NNP 10_1101-2021_02_08_430275 155 28 ] ] -RRB- 10_1101-2021_02_08_430275 155 29 , , , 10_1101-2021_02_08_430275 155 30 [ [ -LRB- 10_1101-2021_02_08_430275 155 31 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 155 32 , , , 10_1101-2021_02_08_430275 155 33 ADref2 ADref2 NNP 10_1101-2021_02_08_430275 155 34 ] ] -RRB- 10_1101-2021_02_08_430275 155 35 ] ] -RRB- 10_1101-2021_02_08_430275 155 36 . . . 10_1101-2021_02_08_430275 156 1 The358 the358 CD 10_1101-2021_02_08_430275 156 2 order order NN 10_1101-2021_02_08_430275 156 3 of of IN 10_1101-2021_02_08_430275 156 4 the the DT 10_1101-2021_02_08_430275 156 5 AD ad NN 10_1101-2021_02_08_430275 156 6 values value NNS 10_1101-2021_02_08_430275 156 7 ( ( -LRB- 10_1101-2021_02_08_430275 156 8 REF REF NNP 10_1101-2021_02_08_430275 156 9 / / SYM 10_1101-2021_02_08_430275 156 10 ALT ALT NNP 10_1101-2021_02_08_430275 156 11 reads read VBZ 10_1101-2021_02_08_430275 156 12 ) ) -RRB- 10_1101-2021_02_08_430275 156 13 in in IN 10_1101-2021_02_08_430275 156 14 bulks bulk NNS 10_1101-2021_02_08_430275 156 15 does do VBZ 10_1101-2021_02_08_430275 156 16 not359 not359 RB 10_1101-2021_02_08_430275 156 17 affect affect VB 10_1101-2021_02_08_430275 156 18 the the DT 10_1101-2021_02_08_430275 156 19 P p NN 10_1101-2021_02_08_430275 156 20 - - HYPH 10_1101-2021_02_08_430275 156 21 value value NN 10_1101-2021_02_08_430275 156 22 of of IN 10_1101-2021_02_08_430275 156 23 Fisher Fisher NNP 10_1101-2021_02_08_430275 156 24 ’s ’s POS 10_1101-2021_02_08_430275 156 25 exact exact JJ 10_1101-2021_02_08_430275 156 26 test test NN 10_1101-2021_02_08_430275 156 27 or or CC 10_1101-2021_02_08_430275 156 28 the the DT 10_1101-2021_02_08_430275 156 29 G g NN 10_1101-2021_02_08_430275 156 30 - - HYPH 10_1101-2021_02_08_430275 156 31 statistic statistic NN 10_1101-2021_02_08_430275 156 32 value360 value360 NN 10_1101-2021_02_08_430275 156 33 of of IN 10_1101-2021_02_08_430275 156 34 G g NN 10_1101-2021_02_08_430275 156 35 - - HYPH 10_1101-2021_02_08_430275 156 36 test test NN 10_1101-2021_02_08_430275 156 37 , , , 10_1101-2021_02_08_430275 156 38 which which WDT 10_1101-2021_02_08_430275 156 39 is be VBZ 10_1101-2021_02_08_430275 156 40 why why WRB 10_1101-2021_02_08_430275 156 41 the the DT 10_1101-2021_02_08_430275 156 42 parental parental JJ 10_1101-2021_02_08_430275 156 43 genome genome NNP 10_1101-2021_02_08_430275 156 44 sequences sequences NNPS 10_1101-2021_02_08_430275 156 45 - - HYPH 10_1101-2021_02_08_430275 156 46 guided361 guided361 NNP 10_1101-2021_02_08_430275 156 47 AD ad NN 10_1101-2021_02_08_430275 156 48 swapping swapping NN 10_1101-2021_02_08_430275 156 49 does do VBZ 10_1101-2021_02_08_430275 156 50 not not RB 10_1101-2021_02_08_430275 156 51 alter alter VB 10_1101-2021_02_08_430275 156 52 the the DT 10_1101-2021_02_08_430275 156 53 curve curve NN 10_1101-2021_02_08_430275 156 54 patterns pattern NNS 10_1101-2021_02_08_430275 156 55 of of IN 10_1101-2021_02_08_430275 156 56 both both DT 10_1101-2021_02_08_430275 156 57 methods.362 methods.362 NNP 10_1101-2021_02_08_430275 156 58 Therefore therefore RB 10_1101-2021_02_08_430275 156 59 , , , 10_1101-2021_02_08_430275 156 60 theoretically theoretically RB 10_1101-2021_02_08_430275 156 61 , , , 10_1101-2021_02_08_430275 156 62 parental parental JJ 10_1101-2021_02_08_430275 156 63 genome genome NN 10_1101-2021_02_08_430275 156 64 sequences sequence NNS 10_1101-2021_02_08_430275 156 65 are be VBP 10_1101-2021_02_08_430275 156 66 not363 not363 RB 10_1101-2021_02_08_430275 156 67 required require VBN 10_1101-2021_02_08_430275 156 68 to to TO 10_1101-2021_02_08_430275 156 69 identify identify VB 10_1101-2021_02_08_430275 156 70 genomic genomic JJ 10_1101-2021_02_08_430275 156 71 region region NN 10_1101-2021_02_08_430275 156 72 - - HYPH 10_1101-2021_02_08_430275 156 73 trait trait NN 10_1101-2021_02_08_430275 156 74 associations association NNS 10_1101-2021_02_08_430275 156 75 in in IN 10_1101-2021_02_08_430275 156 76 either364 either364 NNP 10_1101-2021_02_08_430275 156 77 the the DT 10_1101-2021_02_08_430275 156 78 significant significant JJ 10_1101-2021_02_08_430275 156 79 structural structural JJ 10_1101-2021_02_08_430275 156 80 variant variant JJ 10_1101-2021_02_08_430275 156 81 method method NN 10_1101-2021_02_08_430275 156 82 or or CC 10_1101-2021_02_08_430275 156 83 the the DT 10_1101-2021_02_08_430275 156 84 G G NNP 10_1101-2021_02_08_430275 156 85 - - HYPH 10_1101-2021_02_08_430275 156 86 statistic365 statistic365 NNP 10_1101-2021_02_08_430275 156 87 method method NN 10_1101-2021_02_08_430275 156 88 . . . 10_1101-2021_02_08_430275 157 1 366 366 CD 10_1101-2021_02_08_430275 157 2 When when WRB 10_1101-2021_02_08_430275 157 3 the the DT 10_1101-2021_02_08_430275 157 4 parental parental JJ 10_1101-2021_02_08_430275 157 5 genome genome JJ 10_1101-2021_02_08_430275 157 6 sequences sequence NNS 10_1101-2021_02_08_430275 157 7 were be VBD 10_1101-2021_02_08_430275 157 8 used use VBN 10_1101-2021_02_08_430275 157 9 , , , 10_1101-2021_02_08_430275 157 10 AD ad NN 10_1101-2021_02_08_430275 157 11 value value NN 10_1101-2021_02_08_430275 157 12 367 367 CD 10_1101-2021_02_08_430275 157 13 swapping swapping NN 10_1101-2021_02_08_430275 157 14 was be VBD 10_1101-2021_02_08_430275 157 15 performed perform VBN 10_1101-2021_02_08_430275 157 16 for for IN 10_1101-2021_02_08_430275 157 17 the the DT 10_1101-2021_02_08_430275 157 18 SNPs snp NNS 10_1101-2021_02_08_430275 157 19 in in IN 10_1101-2021_02_08_430275 157 20 which which WDT 10_1101-2021_02_08_430275 157 21 the the DT 10_1101-2021_02_08_430275 157 22 genotype genotype NN 10_1101-2021_02_08_430275 157 23 of of IN 10_1101-2021_02_08_430275 157 24 368 368 CD 10_1101-2021_02_08_430275 157 25 LD24 LD24 NNP 10_1101-2021_02_08_430275 157 26 was be VBD 10_1101-2021_02_08_430275 157 27 different different JJ 10_1101-2021_02_08_430275 157 28 from from IN 10_1101-2021_02_08_430275 157 29 the the DT 10_1101-2021_02_08_430275 157 30 REF REF NNP 10_1101-2021_02_08_430275 157 31 base base NN 10_1101-2021_02_08_430275 157 32 , , , 10_1101-2021_02_08_430275 157 33 and and CC 10_1101-2021_02_08_430275 157 34 the the DT 10_1101-2021_02_08_430275 157 35 ΔAF δaf NN 10_1101-2021_02_08_430275 157 36 values value NNS 10_1101-2021_02_08_430275 157 37 of of IN 10_1101-2021_02_08_430275 157 38 369 369 CD 10_1101-2021_02_08_430275 157 39 these these DT 10_1101-2021_02_08_430275 157 40 SNPs snp NNS 10_1101-2021_02_08_430275 157 41 were be VBD 10_1101-2021_02_08_430275 157 42 calculated calculate VBN 10_1101-2021_02_08_430275 157 43 based base VBN 10_1101-2021_02_08_430275 157 44 on on IN 10_1101-2021_02_08_430275 157 45 the the DT 10_1101-2021_02_08_430275 157 46 swapped swap VBN 10_1101-2021_02_08_430275 157 47 AD ad NN 10_1101-2021_02_08_430275 157 48 values value NNS 10_1101-2021_02_08_430275 157 49 370 370 CD 10_1101-2021_02_08_430275 157 50 using use VBG 10_1101-2021_02_08_430275 157 51 equation equation NN 10_1101-2021_02_08_430275 157 52 1 1 CD 10_1101-2021_02_08_430275 157 53 . . . 10_1101-2021_02_08_430275 158 1 AD ad NN 10_1101-2021_02_08_430275 158 2 swapping swapping NN 10_1101-2021_02_08_430275 158 3 makes make VBZ 10_1101-2021_02_08_430275 158 4 the the DT 10_1101-2021_02_08_430275 158 5 adjacent adjacent JJ 10_1101-2021_02_08_430275 158 6 SNP SNP NNP 10_1101-2021_02_08_430275 158 7 alleles allele VBZ 10_1101-2021_02_08_430275 158 8 371 371 CD 10_1101-2021_02_08_430275 158 9 from from IN 10_1101-2021_02_08_430275 158 10 the the DT 10_1101-2021_02_08_430275 158 11 same same JJ 10_1101-2021_02_08_430275 158 12 parent parent NN 10_1101-2021_02_08_430275 158 13 have have VBP 10_1101-2021_02_08_430275 158 14 similar similar JJ 10_1101-2021_02_08_430275 158 15 AD ad NN 10_1101-2021_02_08_430275 158 16 values value NNS 10_1101-2021_02_08_430275 158 17 and and CC 10_1101-2021_02_08_430275 158 18 similar similar JJ 10_1101-2021_02_08_430275 158 19 ΔAF δaf NN 10_1101-2021_02_08_430275 158 20 372 372 CD 10_1101-2021_02_08_430275 158 21 values value NNS 10_1101-2021_02_08_430275 158 22 . . . 10_1101-2021_02_08_430275 159 1 The the DT 10_1101-2021_02_08_430275 159 2 ΔAF δaf NN 10_1101-2021_02_08_430275 159 3 values value NNS 10_1101-2021_02_08_430275 159 4 of of IN 10_1101-2021_02_08_430275 159 5 such such JJ 10_1101-2021_02_08_430275 159 6 SNPs snp NNS 10_1101-2021_02_08_430275 159 7 were be VBD 10_1101-2021_02_08_430275 159 8 calculated calculate VBN 10_1101-2021_02_08_430275 159 9 using use VBG 10_1101-2021_02_08_430275 159 10 373 373 CD 10_1101-2021_02_08_430275 159 11 equation equation NN 10_1101-2021_02_08_430275 159 12 2 2 CD 10_1101-2021_02_08_430275 159 13 if if IN 10_1101-2021_02_08_430275 159 14 not not RB 10_1101-2021_02_08_430275 159 15 performing perform VBG 10_1101-2021_02_08_430275 159 16 AD ad NN 10_1101-2021_02_08_430275 159 17 swapping swapping NN 10_1101-2021_02_08_430275 159 18 . . . 10_1101-2021_02_08_430275 160 1 Equation equation NN 10_1101-2021_02_08_430275 160 2 2 2 CD 10_1101-2021_02_08_430275 160 3 can can MD 10_1101-2021_02_08_430275 160 4 374 374 CD 10_1101-2021_02_08_430275 160 5 be be VB 10_1101-2021_02_08_430275 160 6 converted convert VBN 10_1101-2021_02_08_430275 160 7 to to IN 10_1101-2021_02_08_430275 160 8 equation equation NN 10_1101-2021_02_08_430275 160 9 3 3 CD 10_1101-2021_02_08_430275 160 10 , , , 10_1101-2021_02_08_430275 160 11 which which WDT 10_1101-2021_02_08_430275 160 12 produces produce VBZ 10_1101-2021_02_08_430275 160 13 an an DT 10_1101-2021_02_08_430275 160 14 opposite opposite JJ 10_1101-2021_02_08_430275 160 15 value value NN 10_1101-2021_02_08_430275 160 16 375 375 CD 10_1101-2021_02_08_430275 160 17 relative relative JJ 10_1101-2021_02_08_430275 160 18 to to IN 10_1101-2021_02_08_430275 160 19 that that DT 10_1101-2021_02_08_430275 160 20 produced produce VBN 10_1101-2021_02_08_430275 160 21 by by IN 10_1101-2021_02_08_430275 160 22 equation equation NN 10_1101-2021_02_08_430275 160 23 1 1 CD 10_1101-2021_02_08_430275 160 24 . . . 10_1101-2021_02_08_430275 161 1 For for IN 10_1101-2021_02_08_430275 161 2 two two CD 10_1101-2021_02_08_430275 161 3 adjacent adjacent JJ 10_1101-2021_02_08_430275 161 4 376 376 CD 10_1101-2021_02_08_430275 161 5 SNPs snp NNS 10_1101-2021_02_08_430275 161 6 in in IN 10_1101-2021_02_08_430275 161 7 LD24 LD24 NNP 10_1101-2021_02_08_430275 161 8 , , , 10_1101-2021_02_08_430275 161 9 where where WRB 10_1101-2021_02_08_430275 161 10 one one CD 10_1101-2021_02_08_430275 161 11 SNP SNP NNP 10_1101-2021_02_08_430275 161 12 has have VBZ 10_1101-2021_02_08_430275 161 13 the the DT 10_1101-2021_02_08_430275 161 14 same same JJ 10_1101-2021_02_08_430275 161 15 genotype genotype NN 10_1101-2021_02_08_430275 161 16 as as IN 10_1101-2021_02_08_430275 161 17 the the DT 10_1101-2021_02_08_430275 161 18 377 377 CD 10_1101-2021_02_08_430275 161 19 REF REF NNP 10_1101-2021_02_08_430275 161 20 base base NN 10_1101-2021_02_08_430275 161 21 while while IN 10_1101-2021_02_08_430275 161 22 the the DT 10_1101-2021_02_08_430275 161 23 other other JJ 10_1101-2021_02_08_430275 161 24 has have VBZ 10_1101-2021_02_08_430275 161 25 the the DT 10_1101-2021_02_08_430275 161 26 same same JJ 10_1101-2021_02_08_430275 161 27 genotype genotype NN 10_1101-2021_02_08_430275 161 28 as as IN 10_1101-2021_02_08_430275 161 29 the the DT 10_1101-2021_02_08_430275 161 30 ALT ALT NNP 10_1101-2021_02_08_430275 161 31 378 378 CD 10_1101-2021_02_08_430275 161 32 base base NN 10_1101-2021_02_08_430275 161 33 , , , 10_1101-2021_02_08_430275 161 34 they -PRON- PRP 10_1101-2021_02_08_430275 161 35 would would MD 10_1101-2021_02_08_430275 161 36 have have VB 10_1101-2021_02_08_430275 161 37 opposite opposite JJ 10_1101-2021_02_08_430275 161 38 ΔAF δaf NN 10_1101-2021_02_08_430275 161 39 values value NNS 10_1101-2021_02_08_430275 161 40 if if IN 10_1101-2021_02_08_430275 161 41 AD ad NN 10_1101-2021_02_08_430275 161 42 swapping swap VBG 10_1101-2021_02_08_430275 161 43 379 379 CD 10_1101-2021_02_08_430275 161 44 is be VBZ 10_1101-2021_02_08_430275 161 45 not not RB 10_1101-2021_02_08_430275 161 46 performed perform VBN 10_1101-2021_02_08_430275 161 47 . . . 10_1101-2021_02_08_430275 162 1 For for IN 10_1101-2021_02_08_430275 162 2 the the DT 10_1101-2021_02_08_430275 162 3 SNPs snp NNS 10_1101-2021_02_08_430275 162 4 that that WDT 10_1101-2021_02_08_430275 162 5 do do VBP 10_1101-2021_02_08_430275 162 6 not not RB 10_1101-2021_02_08_430275 162 7 contribute contribute VB 10_1101-2021_02_08_430275 162 8 to to IN 10_1101-2021_02_08_430275 162 9 380 380 CD 10_1101-2021_02_08_430275 162 10 the the DT 10_1101-2021_02_08_430275 162 11 trait trait NN 10_1101-2021_02_08_430275 162 12 phenotype phenotype NN 10_1101-2021_02_08_430275 162 13 and and CC 10_1101-2021_02_08_430275 162 14 are be VBP 10_1101-2021_02_08_430275 162 15 not not RB 10_1101-2021_02_08_430275 162 16 linked link VBN 10_1101-2021_02_08_430275 162 17 to to IN 10_1101-2021_02_08_430275 162 18 any any DT 10_1101-2021_02_08_430275 162 19 trait trait NN 10_1101-2021_02_08_430275 162 20 - - HYPH 10_1101-2021_02_08_430275 162 21 associated associate VBN 10_1101-2021_02_08_430275 162 22 381 381 CD 10_1101-2021_02_08_430275 162 23 genomic genomic JJ 10_1101-2021_02_08_430275 162 24 regions region NNS 10_1101-2021_02_08_430275 162 25 , , , 10_1101-2021_02_08_430275 162 26 their -PRON- PRP$ 10_1101-2021_02_08_430275 162 27 ΔAF δaf NN 10_1101-2021_02_08_430275 162 28 value value NN 10_1101-2021_02_08_430275 162 29 should should MD 10_1101-2021_02_08_430275 162 30 fluctuate fluctuate VB 10_1101-2021_02_08_430275 162 31 around around RB 10_1101-2021_02_08_430275 162 32 382 382 CD 10_1101-2021_02_08_430275 162 33 zero zero CD 10_1101-2021_02_08_430275 162 34 . . . 10_1101-2021_02_08_430275 163 1 The the DT 10_1101-2021_02_08_430275 163 2 parental parental JJ 10_1101-2021_02_08_430275 163 3 genome genome JJ 10_1101-2021_02_08_430275 163 4 sequences sequence NNS 10_1101-2021_02_08_430275 163 5 will will MD 10_1101-2021_02_08_430275 163 6 have have VB 10_1101-2021_02_08_430275 163 7 less less JJR 10_1101-2021_02_08_430275 163 8 effect effect NN 10_1101-2021_02_08_430275 163 9 383 383 CD 10_1101-2021_02_08_430275 163 10 on on IN 10_1101-2021_02_08_430275 163 11 the the DT 10_1101-2021_02_08_430275 163 12 ΔAF δaf NN 10_1101-2021_02_08_430275 163 13 value value NN 10_1101-2021_02_08_430275 163 14 of of IN 10_1101-2021_02_08_430275 163 15 the the DT 10_1101-2021_02_08_430275 163 16 sliding slide VBG 10_1101-2021_02_08_430275 163 17 windows window NNS 10_1101-2021_02_08_430275 163 18 containing contain VBG 10_1101-2021_02_08_430275 163 19 such such JJ 10_1101-2021_02_08_430275 163 20 384 384 CD 10_1101-2021_02_08_430275 163 21 SNPs snp NNS 10_1101-2021_02_08_430275 163 22 . . . 10_1101-2021_02_08_430275 164 1 However however RB 10_1101-2021_02_08_430275 164 2 , , , 10_1101-2021_02_08_430275 164 3 for for IN 10_1101-2021_02_08_430275 164 4 trait trait NN 10_1101-2021_02_08_430275 164 5 - - HYPH 10_1101-2021_02_08_430275 164 6 associated associate VBN 10_1101-2021_02_08_430275 164 7 SNPs snp NNS 10_1101-2021_02_08_430275 164 8 , , , 10_1101-2021_02_08_430275 164 9 adjacent adjacent JJ 10_1101-2021_02_08_430275 164 10 SNPs snp NNS 10_1101-2021_02_08_430275 164 11 385 385 CD 10_1101-2021_02_08_430275 164 12 with with IN 10_1101-2021_02_08_430275 164 13 opposite opposite JJ 10_1101-2021_02_08_430275 164 14 ΔAF δaf NN 10_1101-2021_02_08_430275 164 15 values value NNS 10_1101-2021_02_08_430275 164 16 would would MD 10_1101-2021_02_08_430275 164 17 cancel cancel VB 10_1101-2021_02_08_430275 164 18 each each DT 10_1101-2021_02_08_430275 164 19 other other JJ 10_1101-2021_02_08_430275 164 20 out out RP 10_1101-2021_02_08_430275 164 21 and and CC 10_1101-2021_02_08_430275 164 22 386 386 CD 10_1101-2021_02_08_430275 164 23 lower low JJR 10_1101-2021_02_08_430275 164 24 the the DT 10_1101-2021_02_08_430275 164 25 ΔAF δaf NN 10_1101-2021_02_08_430275 164 26 value value NN 10_1101-2021_02_08_430275 164 27 of of IN 10_1101-2021_02_08_430275 164 28 the the DT 10_1101-2021_02_08_430275 164 29 sliding slide VBG 10_1101-2021_02_08_430275 164 30 window window NN 10_1101-2021_02_08_430275 164 31 significantly significantly RB 10_1101-2021_02_08_430275 164 32 , , , 10_1101-2021_02_08_430275 164 33 which which WDT 10_1101-2021_02_08_430275 164 34 387 387 CD 10_1101-2021_02_08_430275 164 35 is be VBZ 10_1101-2021_02_08_430275 164 36 the the DT 10_1101-2021_02_08_430275 164 37 case case NN 10_1101-2021_02_08_430275 164 38 observed observe VBN 10_1101-2021_02_08_430275 164 39 on on IN 10_1101-2021_02_08_430275 164 40 chromosome chromosome NN 10_1101-2021_02_08_430275 164 41 11 11 CD 10_1101-2021_02_08_430275 164 42 in in IN 10_1101-2021_02_08_430275 164 43 Figure Figure NNP 10_1101-2021_02_08_430275 164 44 2d 2d NNP 10_1101-2021_02_08_430275 164 45 . . . 10_1101-2021_02_08_430275 165 1 388 388 CD 10_1101-2021_02_08_430275 165 2 ∆AF ∆AF NNP 10_1101-2021_02_08_430275 165 3 = = SYM 10_1101-2021_02_08_430275 165 4 ADref ADref NNP 10_1101-2021_02_08_430275 165 5 2 2 CD 10_1101-2021_02_08_430275 165 6 ADref ADref NNP 10_1101-2021_02_08_430275 165 7 2 2 CD 10_1101-2021_02_08_430275 165 8 + + CC 10_1101-2021_02_08_430275 165 9 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 165 10 − − NNP 10_1101-2021_02_08_430275 165 11 ADref ADref NNP 10_1101-2021_02_08_430275 165 12 1 1 CD 10_1101-2021_02_08_430275 165 13 ADref ADref NNP 10_1101-2021_02_08_430275 165 14 1 1 CD 10_1101-2021_02_08_430275 165 15 + + SYM 10_1101-2021_02_08_430275 165 16 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 165 17 [ [ -LRB- 10_1101-2021_02_08_430275 165 18 2 2 CD 10_1101-2021_02_08_430275 165 19 ] ] -RRB- 10_1101-2021_02_08_430275 165 20 389 389 CD 10_1101-2021_02_08_430275 165 21 Zhang Zhang NNP 10_1101-2021_02_08_430275 165 22 et et FW 10_1101-2021_02_08_430275 165 23 al al NNP 10_1101-2021_02_08_430275 165 24 . . . 10_1101-2021_02_08_430275 166 1 bioRχiv bioRχiv NNP 10_1101-2021_02_08_430275 166 2 | | NNP 10_1101-2021_02_08_430275 166 3 November November NNP 10_1101-2021_02_08_430275 166 4 24 24 CD 10_1101-2021_02_08_430275 166 5 , , , 10_1101-2021_02_08_430275 166 6 2020 2020 CD 10_1101-2021_02_08_430275 166 7 | | CD 10_1101-2021_02_08_430275 166 8 5 5 CD 10_1101-2021_02_08_430275 166 9 .CC .CC , 10_1101-2021_02_08_430275 166 10 - - HYPH 10_1101-2021_02_08_430275 166 11 BY by IN 10_1101-2021_02_08_430275 166 12 - - HYPH 10_1101-2021_02_08_430275 166 13 NC NC NNP 10_1101-2021_02_08_430275 166 14 - - HYPH 10_1101-2021_02_08_430275 166 15 ND ND NNP 10_1101-2021_02_08_430275 166 16 4.0 4.0 CD 10_1101-2021_02_08_430275 166 17 International International NNP 10_1101-2021_02_08_430275 166 18 licenseavailable licenseavailable NN 10_1101-2021_02_08_430275 166 19 under under IN 10_1101-2021_02_08_430275 166 20 a a DT 10_1101-2021_02_08_430275 166 21 ( ( -LRB- 10_1101-2021_02_08_430275 166 22 which which WDT 10_1101-2021_02_08_430275 166 23 was be VBD 10_1101-2021_02_08_430275 166 24 not not RB 10_1101-2021_02_08_430275 166 25 certified certify VBN 10_1101-2021_02_08_430275 166 26 by by IN 10_1101-2021_02_08_430275 166 27 peer peer NN 10_1101-2021_02_08_430275 166 28 review review NN 10_1101-2021_02_08_430275 166 29 ) ) -RRB- 10_1101-2021_02_08_430275 166 30 is be VBZ 10_1101-2021_02_08_430275 166 31 the the DT 10_1101-2021_02_08_430275 166 32 author author NN 10_1101-2021_02_08_430275 166 33 / / SYM 10_1101-2021_02_08_430275 166 34 funder funder NN 10_1101-2021_02_08_430275 166 35 , , , 10_1101-2021_02_08_430275 166 36 who who WP 10_1101-2021_02_08_430275 166 37 has have VBZ 10_1101-2021_02_08_430275 166 38 granted grant VBN 10_1101-2021_02_08_430275 166 39 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 166 40 a a DT 10_1101-2021_02_08_430275 166 41 license license NN 10_1101-2021_02_08_430275 166 42 to to TO 10_1101-2021_02_08_430275 166 43 display display VB 10_1101-2021_02_08_430275 166 44 the the DT 10_1101-2021_02_08_430275 166 45 preprint preprint NN 10_1101-2021_02_08_430275 166 46 in in IN 10_1101-2021_02_08_430275 166 47 perpetuity perpetuity NN 10_1101-2021_02_08_430275 166 48 . . . 10_1101-2021_02_08_430275 167 1 It -PRON- PRP 10_1101-2021_02_08_430275 167 2 is be VBZ 10_1101-2021_02_08_430275 167 3 made make VBN 10_1101-2021_02_08_430275 167 4 The the DT 10_1101-2021_02_08_430275 167 5 copyright copyright NN 10_1101-2021_02_08_430275 167 6 holder holder NN 10_1101-2021_02_08_430275 167 7 for for IN 10_1101-2021_02_08_430275 167 8 this this DT 10_1101-2021_02_08_430275 167 9 preprintthis preprintthis NN 10_1101-2021_02_08_430275 167 10 version version NN 10_1101-2021_02_08_430275 167 11 posted post VBD 10_1101-2021_02_08_430275 167 12 February February NNP 10_1101-2021_02_08_430275 167 13 10 10 CD 10_1101-2021_02_08_430275 167 14 , , , 10_1101-2021_02_08_430275 167 15 2021 2021 CD 10_1101-2021_02_08_430275 167 16 . . . 10_1101-2021_02_08_430275 167 17 ; ; : 10_1101-2021_02_08_430275 167 18 https://doi.org/10.1101/2021.02.08.430275doi https://doi.org/10.1101/2021.02.08.430275doi ADD 10_1101-2021_02_08_430275 167 19 : : : 10_1101-2021_02_08_430275 167 20 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 167 21 preprint preprint NN 10_1101-2021_02_08_430275 167 22 https://doi.org/10.1101/2021.02.08.430275 https://doi.org/10.1101/2021.02.08.430275 NNP 10_1101-2021_02_08_430275 167 23 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_02_08_430275 167 24 DR DR NNP 10_1101-2021_02_08_430275 167 25 AF AF NNP 10_1101-2021_02_08_430275 167 26 T T NNP 10_1101-2021_02_08_430275 167 27 ∆AF ∆AF NNP 10_1101-2021_02_08_430275 167 28 = = SYM 10_1101-2021_02_08_430275 167 29 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 167 30 ADref ADref NNP 10_1101-2021_02_08_430275 167 31 1 1 CD 10_1101-2021_02_08_430275 167 32 + + SYM 10_1101-2021_02_08_430275 167 33 ADalt1 ADalt1 NNP 10_1101-2021_02_08_430275 167 34 − − NNP 10_1101-2021_02_08_430275 167 35 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 167 36 ADref ADref NNP 10_1101-2021_02_08_430275 167 37 2 2 CD 10_1101-2021_02_08_430275 167 38 + + SYM 10_1101-2021_02_08_430275 167 39 ADalt2 ADalt2 NNP 10_1101-2021_02_08_430275 167 40 [ [ -LRB- 10_1101-2021_02_08_430275 167 41 3]390 3]390 CD 10_1101-2021_02_08_430275 167 42 When when WRB 10_1101-2021_02_08_430275 167 43 the the DT 10_1101-2021_02_08_430275 167 44 parental parental JJ 10_1101-2021_02_08_430275 167 45 genome genome NN 10_1101-2021_02_08_430275 167 46 sequences sequence NNS 10_1101-2021_02_08_430275 167 47 were be VBD 10_1101-2021_02_08_430275 167 48 not not RB 10_1101-2021_02_08_430275 167 49 used use VBN 10_1101-2021_02_08_430275 167 50 , , , 10_1101-2021_02_08_430275 167 51 the391 the391 LS 10_1101-2021_02_08_430275 167 52 sSNP ssnp NN 10_1101-2021_02_08_430275 167 53 / / SYM 10_1101-2021_02_08_430275 167 54 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 167 55 ratios ratio NNS 10_1101-2021_02_08_430275 167 56 and and CC 10_1101-2021_02_08_430275 167 57 the the DT 10_1101-2021_02_08_430275 167 58 G g NN 10_1101-2021_02_08_430275 167 59 - - HYPH 10_1101-2021_02_08_430275 167 60 statistic statistic NN 10_1101-2021_02_08_430275 167 61 values value NNS 10_1101-2021_02_08_430275 167 62 were be VBD 10_1101-2021_02_08_430275 167 63 signifi-392 signifi-392 JJ 10_1101-2021_02_08_430275 167 64 cantly cantly RB 10_1101-2021_02_08_430275 167 65 lower low JJR 10_1101-2021_02_08_430275 167 66 . . . 10_1101-2021_02_08_430275 168 1 The the DT 10_1101-2021_02_08_430275 168 2 peak peak NN 10_1101-2021_02_08_430275 168 3 sSNP ssnp NN 10_1101-2021_02_08_430275 168 4 / / SYM 10_1101-2021_02_08_430275 168 5 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 168 6 ratio ratio NN 10_1101-2021_02_08_430275 168 7 on on IN 10_1101-2021_02_08_430275 168 8 chromosome393 chromosome393 NNP 10_1101-2021_02_08_430275 168 9 11 11 CD 10_1101-2021_02_08_430275 168 10 was be VBD 10_1101-2021_02_08_430275 168 11 0.5926 0.5926 CD 10_1101-2021_02_08_430275 168 12 in in IN 10_1101-2021_02_08_430275 168 13 Figure figure NN 10_1101-2021_02_08_430275 168 14 1b 1b NN 10_1101-2021_02_08_430275 168 15 , , , 10_1101-2021_02_08_430275 168 16 while while IN 10_1101-2021_02_08_430275 168 17 it -PRON- PRP 10_1101-2021_02_08_430275 168 18 was be VBD 10_1101-2021_02_08_430275 168 19 0.3810 0.3810 CD 10_1101-2021_02_08_430275 168 20 in in IN 10_1101-2021_02_08_430275 168 21 Figure Figure NNP 10_1101-2021_02_08_430275 168 22 2b 2b NNS 10_1101-2021_02_08_430275 168 23 ; ; : 10_1101-2021_02_08_430275 168 24 it394 it394 NNP 10_1101-2021_02_08_430275 168 25 was be VBD 10_1101-2021_02_08_430275 168 26 similar similar JJ 10_1101-2021_02_08_430275 168 27 for for IN 10_1101-2021_02_08_430275 168 28 the the DT 10_1101-2021_02_08_430275 168 29 peak peak NN 10_1101-2021_02_08_430275 168 30 G g NN 10_1101-2021_02_08_430275 168 31 - - HYPH 10_1101-2021_02_08_430275 168 32 statistic statistic NN 10_1101-2021_02_08_430275 168 33 values value NNS 10_1101-2021_02_08_430275 168 34 . . . 10_1101-2021_02_08_430275 169 1 The the DT 10_1101-2021_02_08_430275 169 2 decreasing decrease VBG 10_1101-2021_02_08_430275 169 3 of395 of395 NNP 10_1101-2021_02_08_430275 169 4 sSNP ssnp NN 10_1101-2021_02_08_430275 169 5 / / SYM 10_1101-2021_02_08_430275 169 6 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 169 7 ratio ratio NN 10_1101-2021_02_08_430275 169 8 and and CC 10_1101-2021_02_08_430275 169 9 the the DT 10_1101-2021_02_08_430275 169 10 G g NN 10_1101-2021_02_08_430275 169 11 - - HYPH 10_1101-2021_02_08_430275 169 12 statistic statistic NN 10_1101-2021_02_08_430275 169 13 value value NN 10_1101-2021_02_08_430275 169 14 is be VBZ 10_1101-2021_02_08_430275 169 15 likely likely JJ 10_1101-2021_02_08_430275 169 16 caused396 caused396 NN 10_1101-2021_02_08_430275 169 17 by by IN 10_1101-2021_02_08_430275 169 18 sequencing sequence VBG 10_1101-2021_02_08_430275 169 19 artifacts artifact NNS 10_1101-2021_02_08_430275 169 20 and and CC 10_1101-2021_02_08_430275 169 21 heterozygosity heterozygosity NN 10_1101-2021_02_08_430275 169 22 in in IN 10_1101-2021_02_08_430275 169 23 the the DT 10_1101-2021_02_08_430275 169 24 parental397 parental397 NN 10_1101-2021_02_08_430275 169 25 lines line NNS 10_1101-2021_02_08_430275 169 26 . . . 10_1101-2021_02_08_430275 170 1 There there EX 10_1101-2021_02_08_430275 170 2 were be VBD 10_1101-2021_02_08_430275 170 3 1 1 CD 10_1101-2021_02_08_430275 170 4 345 345 CD 10_1101-2021_02_08_430275 170 5 185 185 CD 10_1101-2021_02_08_430275 170 6 SNPs snp NNS 10_1101-2021_02_08_430275 170 7 in in IN 10_1101-2021_02_08_430275 170 8 the the DT 10_1101-2021_02_08_430275 170 9 bulk bulk NN 10_1101-2021_02_08_430275 170 10 dataset dataset NN 10_1101-2021_02_08_430275 170 11 when398 when398 PRP 10_1101-2021_02_08_430275 170 12 not not RB 10_1101-2021_02_08_430275 170 13 using use VBG 10_1101-2021_02_08_430275 170 14 the the DT 10_1101-2021_02_08_430275 170 15 parental parental JJ 10_1101-2021_02_08_430275 170 16 genome genome JJ 10_1101-2021_02_08_430275 170 17 sequences sequence NNS 10_1101-2021_02_08_430275 170 18 , , , 10_1101-2021_02_08_430275 170 19 while while IN 10_1101-2021_02_08_430275 170 20 there there EX 10_1101-2021_02_08_430275 170 21 were399 were399 NNP 10_1101-2021_02_08_430275 170 22 1 1 CD 10_1101-2021_02_08_430275 170 23 099 099 CD 10_1101-2021_02_08_430275 170 24 516 516 CD 10_1101-2021_02_08_430275 170 25 SNPs snp NNS 10_1101-2021_02_08_430275 170 26 in in IN 10_1101-2021_02_08_430275 170 27 the the DT 10_1101-2021_02_08_430275 170 28 dataset dataset NN 10_1101-2021_02_08_430275 170 29 with with IN 10_1101-2021_02_08_430275 170 30 the the DT 10_1101-2021_02_08_430275 170 31 aid aid NN 10_1101-2021_02_08_430275 170 32 of of IN 10_1101-2021_02_08_430275 170 33 the the DT 10_1101-2021_02_08_430275 170 34 parental400 parental400 NNP 10_1101-2021_02_08_430275 170 35 genome genome NNP 10_1101-2021_02_08_430275 170 36 sequences sequence NNS 10_1101-2021_02_08_430275 170 37 . . . 10_1101-2021_02_08_430275 171 1 Comparison comparison NN 10_1101-2021_02_08_430275 171 2 of of IN 10_1101-2021_02_08_430275 171 3 the the DT 10_1101-2021_02_08_430275 171 4 two two CD 10_1101-2021_02_08_430275 171 5 SNP SNP NNP 10_1101-2021_02_08_430275 171 6 dataset dataset NN 10_1101-2021_02_08_430275 171 7 re-401 re-401 NNP 10_1101-2021_02_08_430275 171 8 vealed veale VBD 10_1101-2021_02_08_430275 171 9 that that IN 10_1101-2021_02_08_430275 171 10 109 109 CD 10_1101-2021_02_08_430275 171 11 445 445 CD 10_1101-2021_02_08_430275 171 12 SNPs snp NNS 10_1101-2021_02_08_430275 171 13 were be VBD 10_1101-2021_02_08_430275 171 14 unique unique JJ 10_1101-2021_02_08_430275 171 15 to to IN 10_1101-2021_02_08_430275 171 16 the the DT 10_1101-2021_02_08_430275 171 17 bulks bulk NNS 10_1101-2021_02_08_430275 171 18 . . . 10_1101-2021_02_08_430275 172 1 Because402 because402 VB 10_1101-2021_02_08_430275 172 2 all all PDT 10_1101-2021_02_08_430275 172 3 the the DT 10_1101-2021_02_08_430275 172 4 SNPs snp NNS 10_1101-2021_02_08_430275 172 5 in in IN 10_1101-2021_02_08_430275 172 6 the the DT 10_1101-2021_02_08_430275 172 7 bulks bulk NNS 10_1101-2021_02_08_430275 172 8 are be VBP 10_1101-2021_02_08_430275 172 9 derived derive VBN 10_1101-2021_02_08_430275 172 10 from from IN 10_1101-2021_02_08_430275 172 11 the the DT 10_1101-2021_02_08_430275 172 12 parental parental JJ 10_1101-2021_02_08_430275 172 13 lines,403 lines,403 NNP 10_1101-2021_02_08_430275 172 14 crossing crossing NN 10_1101-2021_02_08_430275 172 15 should should MD 10_1101-2021_02_08_430275 172 16 not not RB 10_1101-2021_02_08_430275 172 17 generate generate VB 10_1101-2021_02_08_430275 172 18 new new JJ 10_1101-2021_02_08_430275 172 19 SNPs snp NNS 10_1101-2021_02_08_430275 172 20 ; ; : 10_1101-2021_02_08_430275 172 21 thus thus RB 10_1101-2021_02_08_430275 172 22 this this DT 10_1101-2021_02_08_430275 172 23 category404 category404 NNP 10_1101-2021_02_08_430275 172 24 of of IN 10_1101-2021_02_08_430275 172 25 SNPs SNPs NNP 10_1101-2021_02_08_430275 172 26 was be VBD 10_1101-2021_02_08_430275 172 27 most most RBS 10_1101-2021_02_08_430275 172 28 likely likely RB 10_1101-2021_02_08_430275 172 29 caused cause VBN 10_1101-2021_02_08_430275 172 30 by by IN 10_1101-2021_02_08_430275 172 31 sequencing sequence VBG 10_1101-2021_02_08_430275 172 32 artifacts artifact NNS 10_1101-2021_02_08_430275 172 33 . . . 10_1101-2021_02_08_430275 173 1 The405 the405 CD 10_1101-2021_02_08_430275 173 2 sequencing sequencing NN 10_1101-2021_02_08_430275 173 3 coverage coverage NN 10_1101-2021_02_08_430275 173 4 in in IN 10_1101-2021_02_08_430275 173 5 the the DT 10_1101-2021_02_08_430275 173 6 bulk bulk NN 10_1101-2021_02_08_430275 173 7 was be VBD 10_1101-2021_02_08_430275 173 8 less less JJR 10_1101-2021_02_08_430275 173 9 than than IN 10_1101-2021_02_08_430275 173 10 eight eight CD 10_1101-2021_02_08_430275 173 11 , , , 10_1101-2021_02_08_430275 173 12 which which WDT 10_1101-2021_02_08_430275 173 13 is406 is406 NNP 10_1101-2021_02_08_430275 173 14 very very RB 10_1101-2021_02_08_430275 173 15 low low JJ 10_1101-2021_02_08_430275 173 16 . . . 10_1101-2021_02_08_430275 174 1 Higher high JJR 10_1101-2021_02_08_430275 174 2 sequencing sequencing NN 10_1101-2021_02_08_430275 174 3 coverage coverage NN 10_1101-2021_02_08_430275 174 4 would would MD 10_1101-2021_02_08_430275 174 5 help help VB 10_1101-2021_02_08_430275 174 6 decrease decrease VB 10_1101-2021_02_08_430275 174 7 the407 the407 NN 10_1101-2021_02_08_430275 174 8 number number NN 10_1101-2021_02_08_430275 174 9 of of IN 10_1101-2021_02_08_430275 174 10 SNPs snp NNS 10_1101-2021_02_08_430275 174 11 derived derive VBN 10_1101-2021_02_08_430275 174 12 from from IN 10_1101-2021_02_08_430275 174 13 sequence sequence NN 10_1101-2021_02_08_430275 174 14 artifacts artifact NNS 10_1101-2021_02_08_430275 174 15 . . . 10_1101-2021_02_08_430275 175 1 Additionally,408 Additionally,408 NNP 10_1101-2021_02_08_430275 175 2 137 137 CD 10_1101-2021_02_08_430275 175 3 224 224 CD 10_1101-2021_02_08_430275 175 4 SNP SNP NNP 10_1101-2021_02_08_430275 175 5 were be VBD 10_1101-2021_02_08_430275 175 6 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 175 7 in in IN 10_1101-2021_02_08_430275 175 8 the the DT 10_1101-2021_02_08_430275 175 9 parental parental JJ 10_1101-2021_02_08_430275 175 10 lines line NNS 10_1101-2021_02_08_430275 175 11 . . . 10_1101-2021_02_08_430275 176 1 Without409 Without409 NNP 10_1101-2021_02_08_430275 176 2 the the DT 10_1101-2021_02_08_430275 176 3 parental parental JJ 10_1101-2021_02_08_430275 176 4 genome genome JJ 10_1101-2021_02_08_430275 176 5 sequences sequence NNS 10_1101-2021_02_08_430275 176 6 , , , 10_1101-2021_02_08_430275 176 7 this this DT 10_1101-2021_02_08_430275 176 8 category category NN 10_1101-2021_02_08_430275 176 9 of of IN 10_1101-2021_02_08_430275 176 10 SNPs SNPs NNPS 10_1101-2021_02_08_430275 176 11 could410 could410 NNP 10_1101-2021_02_08_430275 176 12 not not RB 10_1101-2021_02_08_430275 176 13 be be VB 10_1101-2021_02_08_430275 176 14 filtered filter VBN 10_1101-2021_02_08_430275 176 15 out out RP 10_1101-2021_02_08_430275 176 16 from from IN 10_1101-2021_02_08_430275 176 17 the the DT 10_1101-2021_02_08_430275 176 18 bulk bulk JJ 10_1101-2021_02_08_430275 176 19 SNP SNP NNP 10_1101-2021_02_08_430275 176 20 dataset dataset NN 10_1101-2021_02_08_430275 176 21 . . . 10_1101-2021_02_08_430275 177 1 However however RB 10_1101-2021_02_08_430275 177 2 , , , 10_1101-2021_02_08_430275 177 3 these411 these411 NNP 10_1101-2021_02_08_430275 177 4 SNPs snp NNS 10_1101-2021_02_08_430275 177 5 can can MD 10_1101-2021_02_08_430275 177 6 be be VB 10_1101-2021_02_08_430275 177 7 decreased decrease VBN 10_1101-2021_02_08_430275 177 8 via via IN 10_1101-2021_02_08_430275 177 9 selfing selfe VBG 10_1101-2021_02_08_430275 177 10 the the DT 10_1101-2021_02_08_430275 177 11 parental parental JJ 10_1101-2021_02_08_430275 177 12 line line NN 10_1101-2021_02_08_430275 177 13 more more JJR 10_1101-2021_02_08_430275 177 14 gener-412 gener-412 JJ 10_1101-2021_02_08_430275 177 15 ations ation NNS 10_1101-2021_02_08_430275 177 16 : : : 10_1101-2021_02_08_430275 177 17 five five CD 10_1101-2021_02_08_430275 177 18 - - HYPH 10_1101-2021_02_08_430275 177 19 generations generation NNS 10_1101-2021_02_08_430275 177 20 selfing selfing NN 10_1101-2021_02_08_430275 177 21 can can MD 10_1101-2021_02_08_430275 177 22 decrease decrease VB 10_1101-2021_02_08_430275 177 23 the the DT 10_1101-2021_02_08_430275 177 24 heterozygosity413 heterozygosity413 NNP 10_1101-2021_02_08_430275 177 25 of of IN 10_1101-2021_02_08_430275 177 26 both both CC 10_1101-2021_02_08_430275 177 27 parental parental JJ 10_1101-2021_02_08_430275 177 28 lines line NNS 10_1101-2021_02_08_430275 177 29 to to IN 10_1101-2021_02_08_430275 177 30 a a DT 10_1101-2021_02_08_430275 177 31 maximum maximum NN 10_1101-2021_02_08_430275 177 32 of of IN 10_1101-2021_02_08_430275 177 33 6.25%.414 6.25%.414 CD 10_1101-2021_02_08_430275 177 34 To to TO 10_1101-2021_02_08_430275 177 35 determine determine VB 10_1101-2021_02_08_430275 177 36 how how WRB 10_1101-2021_02_08_430275 177 37 parental parental JJ 10_1101-2021_02_08_430275 177 38 heterozygosity heterozygosity NN 10_1101-2021_02_08_430275 177 39 and and CC 10_1101-2021_02_08_430275 177 40 sequenc-415 sequenc-415 JJ 10_1101-2021_02_08_430275 177 41 ing ing NNP 10_1101-2021_02_08_430275 177 42 artifacts artifact NNS 10_1101-2021_02_08_430275 177 43 affected affect VBD 10_1101-2021_02_08_430275 177 44 the the DT 10_1101-2021_02_08_430275 177 45 detection detection NN 10_1101-2021_02_08_430275 177 46 of of IN 10_1101-2021_02_08_430275 177 47 genomic genomic JJ 10_1101-2021_02_08_430275 177 48 region region NN 10_1101-2021_02_08_430275 177 49 - - HYPH 10_1101-2021_02_08_430275 177 50 trait416 trait416 NNP 10_1101-2021_02_08_430275 177 51 associations association NNS 10_1101-2021_02_08_430275 177 52 , , , 10_1101-2021_02_08_430275 177 53 we -PRON- PRP 10_1101-2021_02_08_430275 177 54 removed remove VBD 10_1101-2021_02_08_430275 177 55 the the DT 10_1101-2021_02_08_430275 177 56 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 177 57 SNPs snp NNS 10_1101-2021_02_08_430275 177 58 or or CC 10_1101-2021_02_08_430275 177 59 the the DT 10_1101-2021_02_08_430275 177 60 bulk-417 bulk-417 NN 10_1101-2021_02_08_430275 177 61 specific specific JJ 10_1101-2021_02_08_430275 177 62 SNPs snp NNS 10_1101-2021_02_08_430275 177 63 from from IN 10_1101-2021_02_08_430275 177 64 the the DT 10_1101-2021_02_08_430275 177 65 bulk bulk JJ 10_1101-2021_02_08_430275 177 66 SNP SNP NNP 10_1101-2021_02_08_430275 177 67 dataset dataset NN 10_1101-2021_02_08_430275 177 68 , , , 10_1101-2021_02_08_430275 177 69 and and CC 10_1101-2021_02_08_430275 177 70 analyzed analyze VBD 10_1101-2021_02_08_430275 177 71 the418 the418 JJ 10_1101-2021_02_08_430275 177 72 data datum NNS 10_1101-2021_02_08_430275 177 73 separately separately RB 10_1101-2021_02_08_430275 177 74 . . . 10_1101-2021_02_08_430275 178 1 By by IN 10_1101-2021_02_08_430275 178 2 removing remove VBG 10_1101-2021_02_08_430275 178 3 the the DT 10_1101-2021_02_08_430275 178 4 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 178 5 SNPs snp NNS 10_1101-2021_02_08_430275 178 6 , , , 10_1101-2021_02_08_430275 178 7 the419 the419 ADD 10_1101-2021_02_08_430275 178 8 peak peak NN 10_1101-2021_02_08_430275 178 9 on on IN 10_1101-2021_02_08_430275 178 10 chromosome chromosome NN 10_1101-2021_02_08_430275 178 11 11 11 CD 10_1101-2021_02_08_430275 178 12 was be VBD 10_1101-2021_02_08_430275 178 13 shifted shift VBN 10_1101-2021_02_08_430275 178 14 to to IN 10_1101-2021_02_08_430275 178 15 26.28 26.28 CD 10_1101-2021_02_08_430275 178 16 Mb Mb NNP 10_1101-2021_02_08_430275 178 17 for for IN 10_1101-2021_02_08_430275 178 18 both420 both420 NNP 10_1101-2021_02_08_430275 178 19 the the DT 10_1101-2021_02_08_430275 178 20 sSNP ssnp NN 10_1101-2021_02_08_430275 178 21 / / SYM 10_1101-2021_02_08_430275 178 22 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 178 23 ratio ratio NN 10_1101-2021_02_08_430275 178 24 and and CC 10_1101-2021_02_08_430275 178 25 the the DT 10_1101-2021_02_08_430275 178 26 G g NN 10_1101-2021_02_08_430275 178 27 - - HYPH 10_1101-2021_02_08_430275 178 28 statistic statistic NN 10_1101-2021_02_08_430275 178 29 value value NN 10_1101-2021_02_08_430275 178 30 , , , 10_1101-2021_02_08_430275 178 31 and and CC 10_1101-2021_02_08_430275 178 32 the421 the421 NN 10_1101-2021_02_08_430275 178 33 sSNP ssnp NN 10_1101-2021_02_08_430275 178 34 / / SYM 10_1101-2021_02_08_430275 178 35 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 178 36 ratio ratio NN 10_1101-2021_02_08_430275 178 37 of of IN 10_1101-2021_02_08_430275 178 38 the the DT 10_1101-2021_02_08_430275 178 39 peak peak NN 10_1101-2021_02_08_430275 178 40 was be VBD 10_1101-2021_02_08_430275 178 41 increased increase VBN 10_1101-2021_02_08_430275 178 42 to to IN 10_1101-2021_02_08_430275 178 43 0.4835,422 0.4835,422 CD 10_1101-2021_02_08_430275 178 44 well well RB 10_1101-2021_02_08_430275 178 45 above above IN 10_1101-2021_02_08_430275 178 46 the the DT 10_1101-2021_02_08_430275 178 47 sliding slide VBG 10_1101-2021_02_08_430275 178 48 window window NN 10_1101-2021_02_08_430275 178 49 - - HYPH 10_1101-2021_02_08_430275 178 50 specific specific JJ 10_1101-2021_02_08_430275 178 51 threshold threshold NN 10_1101-2021_02_08_430275 178 52 0.0603 0.0603 CD 10_1101-2021_02_08_430275 178 53 . . . 10_1101-2021_02_08_430275 179 1 The423 the423 ADD 10_1101-2021_02_08_430275 179 2 G g NN 10_1101-2021_02_08_430275 179 3 - - HYPH 10_1101-2021_02_08_430275 179 4 statistic statistic NN 10_1101-2021_02_08_430275 179 5 value value NN 10_1101-2021_02_08_430275 179 6 of of IN 10_1101-2021_02_08_430275 179 7 the the DT 10_1101-2021_02_08_430275 179 8 peak peak NN 10_1101-2021_02_08_430275 179 9 was be VBD 10_1101-2021_02_08_430275 179 10 10.8411 10.8411 CD 10_1101-2021_02_08_430275 179 11 , , , 10_1101-2021_02_08_430275 179 12 significantly significantly RB 10_1101-2021_02_08_430275 179 13 higher424 higher424 NNP 10_1101-2021_02_08_430275 179 14 than than IN 10_1101-2021_02_08_430275 179 15 the the DT 10_1101-2021_02_08_430275 179 16 threshold threshold NN 10_1101-2021_02_08_430275 179 17 8.9532 8.9532 CD 10_1101-2021_02_08_430275 179 18 as as RB 10_1101-2021_02_08_430275 179 19 well well RB 10_1101-2021_02_08_430275 179 20 . . . 10_1101-2021_02_08_430275 180 1 By by IN 10_1101-2021_02_08_430275 180 2 removing remove VBG 10_1101-2021_02_08_430275 180 3 bulk bulk NN 10_1101-2021_02_08_430275 180 4 - - HYPH 10_1101-2021_02_08_430275 180 5 specific425 specific425 NN 10_1101-2021_02_08_430275 180 6 SNPs snp NNS 10_1101-2021_02_08_430275 180 7 , , , 10_1101-2021_02_08_430275 180 8 the the DT 10_1101-2021_02_08_430275 180 9 peak peak NN 10_1101-2021_02_08_430275 180 10 on on IN 10_1101-2021_02_08_430275 180 11 chromosome chromosome NN 10_1101-2021_02_08_430275 180 12 11 11 CD 10_1101-2021_02_08_430275 180 13 shifted shift VBD 10_1101-2021_02_08_430275 180 14 to to IN 10_1101-2021_02_08_430275 180 15 26.49 26.49 CD 10_1101-2021_02_08_430275 180 16 Mb Mb NNS 10_1101-2021_02_08_430275 180 17 for426 for426 NNP 10_1101-2021_02_08_430275 180 18 both both CC 10_1101-2021_02_08_430275 180 19 the the DT 10_1101-2021_02_08_430275 180 20 sSNP ssnp NN 10_1101-2021_02_08_430275 180 21 / / SYM 10_1101-2021_02_08_430275 180 22 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 180 23 ratio ratio NN 10_1101-2021_02_08_430275 180 24 and and CC 10_1101-2021_02_08_430275 180 25 the the DT 10_1101-2021_02_08_430275 180 26 G g NN 10_1101-2021_02_08_430275 180 27 - - HYPH 10_1101-2021_02_08_430275 180 28 statistic statistic NN 10_1101-2021_02_08_430275 180 29 value value NN 10_1101-2021_02_08_430275 180 30 . . . 10_1101-2021_02_08_430275 181 1 The427 the427 ADD 10_1101-2021_02_08_430275 181 2 sSNP sSNP NNP 10_1101-2021_02_08_430275 181 3 / / SYM 10_1101-2021_02_08_430275 181 4 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 181 5 ratio ratio NN 10_1101-2021_02_08_430275 181 6 of of IN 10_1101-2021_02_08_430275 181 7 the the DT 10_1101-2021_02_08_430275 181 8 peak peak NN 10_1101-2021_02_08_430275 181 9 and and CC 10_1101-2021_02_08_430275 181 10 the the DT 10_1101-2021_02_08_430275 181 11 sliding slide VBG 10_1101-2021_02_08_430275 181 12 window-428 window-428 NNP 10_1101-2021_02_08_430275 181 13 specific specific JJ 10_1101-2021_02_08_430275 181 14 threshold threshold NN 10_1101-2021_02_08_430275 181 15 were be VBD 10_1101-2021_02_08_430275 181 16 0.4302 0.4302 CD 10_1101-2021_02_08_430275 181 17 and and CC 10_1101-2021_02_08_430275 181 18 0.0637 0.0637 CD 10_1101-2021_02_08_430275 181 19 , , , 10_1101-2021_02_08_430275 181 20 respectively respectively RB 10_1101-2021_02_08_430275 181 21 , , , 10_1101-2021_02_08_430275 181 22 and429 and429 NNP 10_1101-2021_02_08_430275 181 23 the the DT 10_1101-2021_02_08_430275 181 24 G g NN 10_1101-2021_02_08_430275 181 25 - - HYPH 10_1101-2021_02_08_430275 181 26 statistic statistic NN 10_1101-2021_02_08_430275 181 27 value value NN 10_1101-2021_02_08_430275 181 28 of of IN 10_1101-2021_02_08_430275 181 29 the the DT 10_1101-2021_02_08_430275 181 30 peak peak NN 10_1101-2021_02_08_430275 181 31 and and CC 10_1101-2021_02_08_430275 181 32 the the DT 10_1101-2021_02_08_430275 181 33 threshold threshold NN 10_1101-2021_02_08_430275 181 34 were be VBD 10_1101-2021_02_08_430275 181 35 9.7591430 9.7591430 CD 10_1101-2021_02_08_430275 181 36 and and CC 10_1101-2021_02_08_430275 181 37 8.9092 8.9092 CD 10_1101-2021_02_08_430275 181 38 , , , 10_1101-2021_02_08_430275 181 39 respectively respectively RB 10_1101-2021_02_08_430275 181 40 . . . 10_1101-2021_02_08_430275 182 1 Although although IN 10_1101-2021_02_08_430275 182 2 both both DT 10_1101-2021_02_08_430275 182 3 the the DT 10_1101-2021_02_08_430275 182 4 sSNP ssnp NN 10_1101-2021_02_08_430275 182 5 / / SYM 10_1101-2021_02_08_430275 182 6 totalSNP431 totalSNP431 NNP 10_1101-2021_02_08_430275 182 7 ratio ratio NN 10_1101-2021_02_08_430275 182 8 and and CC 10_1101-2021_02_08_430275 182 9 the the DT 10_1101-2021_02_08_430275 182 10 G g NN 10_1101-2021_02_08_430275 182 11 - - HYPH 10_1101-2021_02_08_430275 182 12 statistic statistic NN 10_1101-2021_02_08_430275 182 13 value value NN 10_1101-2021_02_08_430275 182 14 were be VBD 10_1101-2021_02_08_430275 182 15 lower low JJR 10_1101-2021_02_08_430275 182 16 than than IN 10_1101-2021_02_08_430275 182 17 above above RB 10_1101-2021_02_08_430275 182 18 , , , 10_1101-2021_02_08_430275 182 19 they432 they432 NN 10_1101-2021_02_08_430275 182 20 were be VBD 10_1101-2021_02_08_430275 182 21 still still RB 10_1101-2021_02_08_430275 182 22 higher high JJR 10_1101-2021_02_08_430275 182 23 than than IN 10_1101-2021_02_08_430275 182 24 their -PRON- PRP$ 10_1101-2021_02_08_430275 182 25 corresponding correspond VBG 10_1101-2021_02_08_430275 182 26 thresholds threshold NNS 10_1101-2021_02_08_430275 182 27 . . . 10_1101-2021_02_08_430275 183 1 While433 While433 NNP 10_1101-2021_02_08_430275 183 2 seemed seem VBD 10_1101-2021_02_08_430275 183 3 the the DT 10_1101-2021_02_08_430275 183 4 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 183 5 SNPs snp NNS 10_1101-2021_02_08_430275 183 6 affected affect VBD 10_1101-2021_02_08_430275 183 7 the the DT 10_1101-2021_02_08_430275 183 8 sSNP ssnp NN 10_1101-2021_02_08_430275 183 9 / / SYM 10_1101-2021_02_08_430275 183 10 totalSNP434 totalSNP434 NNP 10_1101-2021_02_08_430275 183 11 ratio ratio NN 10_1101-2021_02_08_430275 183 12 and and CC 10_1101-2021_02_08_430275 183 13 the the DT 10_1101-2021_02_08_430275 183 14 G g NN 10_1101-2021_02_08_430275 183 15 - - HYPH 10_1101-2021_02_08_430275 183 16 statistic statistic NN 10_1101-2021_02_08_430275 183 17 value value NN 10_1101-2021_02_08_430275 183 18 a a DT 10_1101-2021_02_08_430275 183 19 little little JJ 10_1101-2021_02_08_430275 183 20 more more JJR 10_1101-2021_02_08_430275 183 21 than than IN 10_1101-2021_02_08_430275 183 22 the the DT 10_1101-2021_02_08_430275 183 23 bulk-435 bulk-435 JJ 10_1101-2021_02_08_430275 183 24 specific specific JJ 10_1101-2021_02_08_430275 183 25 SNPs snp NNS 10_1101-2021_02_08_430275 183 26 , , , 10_1101-2021_02_08_430275 183 27 it -PRON- PRP 10_1101-2021_02_08_430275 183 28 is be VBZ 10_1101-2021_02_08_430275 183 29 more more RBR 10_1101-2021_02_08_430275 183 30 likely likely JJ 10_1101-2021_02_08_430275 183 31 that that IN 10_1101-2021_02_08_430275 183 32 both both DT 10_1101-2021_02_08_430275 183 33 produced produce VBD 10_1101-2021_02_08_430275 183 34 similar436 similar436 NN 10_1101-2021_02_08_430275 183 35 levels level NNS 10_1101-2021_02_08_430275 183 36 of of IN 10_1101-2021_02_08_430275 183 37 noise noise NN 10_1101-2021_02_08_430275 183 38 for for IN 10_1101-2021_02_08_430275 183 39 the the DT 10_1101-2021_02_08_430275 183 40 sSNP ssnp NN 10_1101-2021_02_08_430275 183 41 / / SYM 10_1101-2021_02_08_430275 183 42 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 183 43 ratio ratio NN 10_1101-2021_02_08_430275 183 44 and and CC 10_1101-2021_02_08_430275 183 45 the the DT 10_1101-2021_02_08_430275 183 46 G G NNP 10_1101-2021_02_08_430275 183 47 - - HYPH 10_1101-2021_02_08_430275 183 48 statistic437 statistic437 NNP 10_1101-2021_02_08_430275 183 49 value value NN 10_1101-2021_02_08_430275 183 50 considering consider VBG 10_1101-2021_02_08_430275 183 51 that that IN 10_1101-2021_02_08_430275 183 52 the the DT 10_1101-2021_02_08_430275 183 53 former former JJ 10_1101-2021_02_08_430275 183 54 was be VBD 10_1101-2021_02_08_430275 183 55 27 27 CD 10_1101-2021_02_08_430275 183 56 779 779 CD 10_1101-2021_02_08_430275 183 57 greater great JJR 10_1101-2021_02_08_430275 183 58 than438 than438 XX 10_1101-2021_02_08_430275 183 59 the the DT 10_1101-2021_02_08_430275 183 60 latter latter JJ 10_1101-2021_02_08_430275 183 61 . . . 10_1101-2021_02_08_430275 184 1 When when WRB 10_1101-2021_02_08_430275 184 2 using use VBG 10_1101-2021_02_08_430275 184 3 only only RB 10_1101-2021_02_08_430275 184 4 the the DT 10_1101-2021_02_08_430275 184 5 bulk bulk JJ 10_1101-2021_02_08_430275 184 6 genome genome JJ 10_1101-2021_02_08_430275 184 7 sequences sequence NNS 10_1101-2021_02_08_430275 184 8 , , , 10_1101-2021_02_08_430275 184 9 the439 the439 JJS 10_1101-2021_02_08_430275 184 10 sSNP ssnp NN 10_1101-2021_02_08_430275 184 11 / / SYM 10_1101-2021_02_08_430275 184 12 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 184 13 peak peak NN 10_1101-2021_02_08_430275 184 14 position position NN 10_1101-2021_02_08_430275 184 15 on on IN 10_1101-2021_02_08_430275 184 16 chromosome chromosome NN 10_1101-2021_02_08_430275 184 17 11 11 CD 10_1101-2021_02_08_430275 184 18 was be VBD 10_1101-2021_02_08_430275 184 19 shifted440 shifted440 NNP 10_1101-2021_02_08_430275 184 20 0.52 0.52 CD 10_1101-2021_02_08_430275 184 21 Mb Mb NNP 10_1101-2021_02_08_430275 184 22 ( ( -LRB- 10_1101-2021_02_08_430275 184 23 26.44 26.44 CD 10_1101-2021_02_08_430275 184 24 Mb mb NN 10_1101-2021_02_08_430275 184 25 to to IN 10_1101-2021_02_08_430275 184 26 26.96 26.96 CD 10_1101-2021_02_08_430275 184 27 Mb Mb NNS 10_1101-2021_02_08_430275 184 28 ) ) -RRB- 10_1101-2021_02_08_430275 184 29 due due IN 10_1101-2021_02_08_430275 184 30 to to IN 10_1101-2021_02_08_430275 184 31 the the DT 10_1101-2021_02_08_430275 184 32 presence presence NN 10_1101-2021_02_08_430275 184 33 of of IN 10_1101-2021_02_08_430275 184 34 the441 the441 JJ 10_1101-2021_02_08_430275 184 35 bulk bulk JJ 10_1101-2021_02_08_430275 184 36 - - HYPH 10_1101-2021_02_08_430275 184 37 specific specific JJ 10_1101-2021_02_08_430275 184 38 SNPs snp NNS 10_1101-2021_02_08_430275 184 39 and and CC 10_1101-2021_02_08_430275 184 40 the the DT 10_1101-2021_02_08_430275 184 41 heterozygous heterozygous JJ 10_1101-2021_02_08_430275 184 42 SNPs snp NNS 10_1101-2021_02_08_430275 184 43 in in IN 10_1101-2021_02_08_430275 184 44 the the DT 10_1101-2021_02_08_430275 184 45 dataset,442 dataset,442 NN 10_1101-2021_02_08_430275 184 46 but but CC 10_1101-2021_02_08_430275 184 47 this this DT 10_1101-2021_02_08_430275 184 48 is be VBZ 10_1101-2021_02_08_430275 184 49 a a DT 10_1101-2021_02_08_430275 184 50 very very RB 10_1101-2021_02_08_430275 184 51 short short JJ 10_1101-2021_02_08_430275 184 52 distance distance NN 10_1101-2021_02_08_430275 184 53 for for IN 10_1101-2021_02_08_430275 184 54 genetic genetic JJ 10_1101-2021_02_08_430275 184 55 mapping mapping NN 10_1101-2021_02_08_430275 184 56 . . . 10_1101-2021_02_08_430275 185 1 Although443 Although443 NNP 10_1101-2021_02_08_430275 185 2 only only RB 10_1101-2021_02_08_430275 185 3 a a DT 10_1101-2021_02_08_430275 185 4 single single JJ 10_1101-2021_02_08_430275 185 5 dataset dataset NN 10_1101-2021_02_08_430275 185 6 was be VBD 10_1101-2021_02_08_430275 185 7 examined examine VBN 10_1101-2021_02_08_430275 185 8 here here RB 10_1101-2021_02_08_430275 185 9 , , , 10_1101-2021_02_08_430275 185 10 the the DT 10_1101-2021_02_08_430275 185 11 genome genome NN 10_1101-2021_02_08_430275 185 12 - - HYPH 10_1101-2021_02_08_430275 185 13 wide444 wide444 NN 10_1101-2021_02_08_430275 185 14 similarity similarity NN 10_1101-2021_02_08_430275 185 15 of of IN 10_1101-2021_02_08_430275 185 16 the the DT 10_1101-2021_02_08_430275 185 17 sSNP ssnp NN 10_1101-2021_02_08_430275 185 18 / / SYM 10_1101-2021_02_08_430275 185 19 totalSNP totalsnp JJ 10_1101-2021_02_08_430275 185 20 curve curve NN 10_1101-2021_02_08_430275 185 21 patterns pattern NNS 10_1101-2021_02_08_430275 185 22 in in IN 10_1101-2021_02_08_430275 185 23 Figure figure NN 10_1101-2021_02_08_430275 185 24 1b445 1b445 CD 10_1101-2021_02_08_430275 185 25 and and CC 10_1101-2021_02_08_430275 185 26 Figure Figure NNP 10_1101-2021_02_08_430275 185 27 2b 2b NNP 10_1101-2021_02_08_430275 185 28 suggests suggest VBZ 10_1101-2021_02_08_430275 185 29 that that IN 10_1101-2021_02_08_430275 185 30 the the DT 10_1101-2021_02_08_430275 185 31 significant significant JJ 10_1101-2021_02_08_430275 185 32 structural structural JJ 10_1101-2021_02_08_430275 185 33 method446 method446 NNP 10_1101-2021_02_08_430275 185 34 is be VBZ 10_1101-2021_02_08_430275 185 35 highly highly RB 10_1101-2021_02_08_430275 185 36 reproducible reproducible JJ 10_1101-2021_02_08_430275 185 37 using use VBG 10_1101-2021_02_08_430275 185 38 only only RB 10_1101-2021_02_08_430275 185 39 the the DT 10_1101-2021_02_08_430275 185 40 bulk bulk NN 10_1101-2021_02_08_430275 185 41 genome genome NN 10_1101-2021_02_08_430275 185 42 sequences.447 sequences.447 NNP 10_1101-2021_02_08_430275 185 43 Conclusions Conclusions NNPS 10_1101-2021_02_08_430275 185 44 448 448 CD 10_1101-2021_02_08_430275 185 45 The the DT 10_1101-2021_02_08_430275 185 46 plotting plot VBG 10_1101-2021_02_08_430275 185 47 pattern pattern NN 10_1101-2021_02_08_430275 185 48 of of IN 10_1101-2021_02_08_430275 185 49 the the DT 10_1101-2021_02_08_430275 185 50 ΔAF δaf NN 10_1101-2021_02_08_430275 185 51 values value NNS 10_1101-2021_02_08_430275 185 52 in in IN 10_1101-2021_02_08_430275 185 53 the the DT 10_1101-2021_02_08_430275 185 54 trait trait NN 10_1101-2021_02_08_430275 185 55 - - HYPH 10_1101-2021_02_08_430275 185 56 associated associate VBN 10_1101-2021_02_08_430275 185 57 449 449 CD 10_1101-2021_02_08_430275 185 58 genomic genomic JJ 10_1101-2021_02_08_430275 185 59 region region NN 10_1101-2021_02_08_430275 185 60 was be VBD 10_1101-2021_02_08_430275 185 61 very very RB 10_1101-2021_02_08_430275 185 62 different different JJ 10_1101-2021_02_08_430275 185 63 when when WRB 10_1101-2021_02_08_430275 185 64 using use VBG 10_1101-2021_02_08_430275 185 65 only only RB 10_1101-2021_02_08_430275 185 66 the the DT 10_1101-2021_02_08_430275 185 67 bulk bulk JJ 10_1101-2021_02_08_430275 185 68 450 450 CD 10_1101-2021_02_08_430275 185 69 genome genome JJ 10_1101-2021_02_08_430275 185 70 sequences sequence NNS 10_1101-2021_02_08_430275 185 71 . . . 10_1101-2021_02_08_430275 186 1 Without without IN 10_1101-2021_02_08_430275 186 2 the the DT 10_1101-2021_02_08_430275 186 3 aid aid NN 10_1101-2021_02_08_430275 186 4 of of IN 10_1101-2021_02_08_430275 186 5 the the DT 10_1101-2021_02_08_430275 186 6 parental parental JJ 10_1101-2021_02_08_430275 186 7 genome genome NNP 10_1101-2021_02_08_430275 186 8 451 451 CD 10_1101-2021_02_08_430275 186 9 sequences sequence NNS 10_1101-2021_02_08_430275 186 10 , , , 10_1101-2021_02_08_430275 186 11 the the DT 10_1101-2021_02_08_430275 186 12 ΔAF δaf NN 10_1101-2021_02_08_430275 186 13 values value NNS 10_1101-2021_02_08_430275 186 14 of of IN 10_1101-2021_02_08_430275 186 15 the the DT 10_1101-2021_02_08_430275 186 16 sliding slide VBG 10_1101-2021_02_08_430275 186 17 windows window NNS 10_1101-2021_02_08_430275 186 18 could could MD 10_1101-2021_02_08_430275 186 19 not not RB 10_1101-2021_02_08_430275 186 20 452 452 CD 10_1101-2021_02_08_430275 186 21 be be VB 10_1101-2021_02_08_430275 186 22 correctly correctly RB 10_1101-2021_02_08_430275 186 23 calculated calculate VBN 10_1101-2021_02_08_430275 186 24 ; ; : 10_1101-2021_02_08_430275 186 25 thus thus RB 10_1101-2021_02_08_430275 186 26 , , , 10_1101-2021_02_08_430275 186 27 the the DT 10_1101-2021_02_08_430275 186 28 allele allele NNP 10_1101-2021_02_08_430275 186 29 frequency frequency NN 10_1101-2021_02_08_430275 186 30 method method NN 10_1101-2021_02_08_430275 186 31 453 453 CD 10_1101-2021_02_08_430275 186 32 can can MD 10_1101-2021_02_08_430275 186 33 not not RB 10_1101-2021_02_08_430275 186 34 be be VB 10_1101-2021_02_08_430275 186 35 used use VBN 10_1101-2021_02_08_430275 186 36 to to TO 10_1101-2021_02_08_430275 186 37 identify identify VB 10_1101-2021_02_08_430275 186 38 SNP SNP NNP 10_1101-2021_02_08_430275 186 39 - - HYPH 10_1101-2021_02_08_430275 186 40 trait trait NNP 10_1101-2021_02_08_430275 186 41 association association NNP 10_1101-2021_02_08_430275 186 42 . . . 10_1101-2021_02_08_430275 187 1 In in IN 10_1101-2021_02_08_430275 187 2 contrast contrast NN 10_1101-2021_02_08_430275 187 3 , , , 10_1101-2021_02_08_430275 187 4 454 454 CD 10_1101-2021_02_08_430275 187 5 the the DT 10_1101-2021_02_08_430275 187 6 parental parental JJ 10_1101-2021_02_08_430275 187 7 genome genome JJ 10_1101-2021_02_08_430275 187 8 sequence sequence NN 10_1101-2021_02_08_430275 187 9 does do VBZ 10_1101-2021_02_08_430275 187 10 not not RB 10_1101-2021_02_08_430275 187 11 affect affect VB 10_1101-2021_02_08_430275 187 12 the the DT 10_1101-2021_02_08_430275 187 13 plotting plot VBG 10_1101-2021_02_08_430275 187 14 455 455 CD 10_1101-2021_02_08_430275 187 15 patterns pattern NNS 10_1101-2021_02_08_430275 187 16 of of IN 10_1101-2021_02_08_430275 187 17 both both CC 10_1101-2021_02_08_430275 187 18 the the DT 10_1101-2021_02_08_430275 187 19 significant significant JJ 10_1101-2021_02_08_430275 187 20 structural structural JJ 10_1101-2021_02_08_430275 187 21 variant variant JJ 10_1101-2021_02_08_430275 187 22 method method NN 10_1101-2021_02_08_430275 187 23 and and CC 10_1101-2021_02_08_430275 187 24 456 456 CD 10_1101-2021_02_08_430275 187 25 the the DT 10_1101-2021_02_08_430275 187 26 G g NN 10_1101-2021_02_08_430275 187 27 - - HYPH 10_1101-2021_02_08_430275 187 28 statistic statistic NN 10_1101-2021_02_08_430275 187 29 method method NN 10_1101-2021_02_08_430275 187 30 , , , 10_1101-2021_02_08_430275 187 31 but but CC 10_1101-2021_02_08_430275 187 32 the the DT 10_1101-2021_02_08_430275 187 33 sSNP ssnp NN 10_1101-2021_02_08_430275 187 34 / / SYM 10_1101-2021_02_08_430275 187 35 totalSNP totalSNP NNP 10_1101-2021_02_08_430275 187 36 ratios ratio NNS 10_1101-2021_02_08_430275 187 37 and and CC 10_1101-2021_02_08_430275 187 38 the the DT 10_1101-2021_02_08_430275 187 39 457 457 CD 10_1101-2021_02_08_430275 187 40 G g NN 10_1101-2021_02_08_430275 187 41 - - HYPH 10_1101-2021_02_08_430275 187 42 statistic statistic NN 10_1101-2021_02_08_430275 187 43 values value NNS 10_1101-2021_02_08_430275 187 44 decreased decrease VBD 10_1101-2021_02_08_430275 187 45 significantly significantly RB 10_1101-2021_02_08_430275 187 46 due due IN 10_1101-2021_02_08_430275 187 47 to to IN 10_1101-2021_02_08_430275 187 48 sequencing sequence VBG 10_1101-2021_02_08_430275 187 49 458 458 CD 10_1101-2021_02_08_430275 187 50 artifacts artifact NNS 10_1101-2021_02_08_430275 187 51 and/or and/or CC 10_1101-2021_02_08_430275 187 52 heterozygosity heterozygosity NN 10_1101-2021_02_08_430275 187 53 of of IN 10_1101-2021_02_08_430275 187 54 the the DT 10_1101-2021_02_08_430275 187 55 parental parental JJ 10_1101-2021_02_08_430275 187 56 lines line NNS 10_1101-2021_02_08_430275 187 57 . . . 10_1101-2021_02_08_430275 188 1 Because because IN 10_1101-2021_02_08_430275 188 2 459 459 CD 10_1101-2021_02_08_430275 188 3 of of IN 10_1101-2021_02_08_430275 188 4 its -PRON- PRP$ 10_1101-2021_02_08_430275 188 5 high high JJ 10_1101-2021_02_08_430275 188 6 detection detection NN 10_1101-2021_02_08_430275 188 7 power power NN 10_1101-2021_02_08_430275 188 8 , , , 10_1101-2021_02_08_430275 188 9 major major JJ 10_1101-2021_02_08_430275 188 10 SNP SNP NNP 10_1101-2021_02_08_430275 188 11 - - HYPH 10_1101-2021_02_08_430275 188 12 trait trait NN 10_1101-2021_02_08_430275 188 13 associations association NNS 10_1101-2021_02_08_430275 188 14 can can MD 10_1101-2021_02_08_430275 188 15 460 460 CD 10_1101-2021_02_08_430275 188 16 still still RB 10_1101-2021_02_08_430275 188 17 be be VB 10_1101-2021_02_08_430275 188 18 reliably reliably RB 10_1101-2021_02_08_430275 188 19 detected detect VBN 10_1101-2021_02_08_430275 188 20 via via IN 10_1101-2021_02_08_430275 188 21 the the DT 10_1101-2021_02_08_430275 188 22 significant significant JJ 10_1101-2021_02_08_430275 188 23 structural structural JJ 10_1101-2021_02_08_430275 188 24 variant variant JJ 10_1101-2021_02_08_430275 188 25 461 461 CD 10_1101-2021_02_08_430275 188 26 method method NN 10_1101-2021_02_08_430275 188 27 even even RB 10_1101-2021_02_08_430275 188 28 the the DT 10_1101-2021_02_08_430275 188 29 sequence sequence NN 10_1101-2021_02_08_430275 188 30 coverage coverage NN 10_1101-2021_02_08_430275 188 31 was be VBD 10_1101-2021_02_08_430275 188 32 very very RB 10_1101-2021_02_08_430275 188 33 low low JJ 10_1101-2021_02_08_430275 188 34 . . . 10_1101-2021_02_08_430275 189 1 462 462 CD 10_1101-2021_02_08_430275 189 2 Acknowledgments acknowledgment NNS 10_1101-2021_02_08_430275 189 3 . . . 10_1101-2021_02_08_430275 190 1 JZ JZ NNP 10_1101-2021_02_08_430275 190 2 was be VBD 10_1101-2021_02_08_430275 190 3 supported support VBN 10_1101-2021_02_08_430275 190 4 by by IN 10_1101-2021_02_08_430275 190 5 the the DT 10_1101-2021_02_08_430275 190 6 National National NNP 10_1101-2021_02_08_430275 190 7 Science Science NNP 10_1101-2021_02_08_430275 190 8 463 463 CD 10_1101-2021_02_08_430275 190 9 Foundation Foundation NNP 10_1101-2021_02_08_430275 190 10 grant grant NN 10_1101-2021_02_08_430275 190 11 [ [ -LRB- 10_1101-2021_02_08_430275 190 12 IOS-1546625 IOS-1546625 NNP 10_1101-2021_02_08_430275 190 13 to to IN 10_1101-2021_02_08_430275 190 14 DRP DRP NNP 10_1101-2021_02_08_430275 190 15 ] ] -RRB- 10_1101-2021_02_08_430275 190 16 . . . 10_1101-2021_02_08_430275 191 1 We -PRON- PRP 10_1101-2021_02_08_430275 191 2 are be VBP 10_1101-2021_02_08_430275 191 3 grateful grateful JJ 10_1101-2021_02_08_430275 191 4 to to IN 10_1101-2021_02_08_430275 191 5 Lahari Lahari NNP 10_1101-2021_02_08_430275 191 6 464 464 CD 10_1101-2021_02_08_430275 191 7 et et FW 10_1101-2021_02_08_430275 191 8 al al NNP 10_1101-2021_02_08_430275 191 9 . . . 10_1101-2021_02_08_430275 192 1 for for IN 10_1101-2021_02_08_430275 192 2 generating generate VBG 10_1101-2021_02_08_430275 192 3 the the DT 10_1101-2021_02_08_430275 192 4 sequencing sequence VBG 10_1101-2021_02_08_430275 192 5 data datum NNS 10_1101-2021_02_08_430275 192 6 and and CC 10_1101-2021_02_08_430275 192 7 making make VBG 10_1101-2021_02_08_430275 192 8 it -PRON- PRP 10_1101-2021_02_08_430275 192 9 available available JJ 10_1101-2021_02_08_430275 192 10 465 465 CD 10_1101-2021_02_08_430275 192 11 to to IN 10_1101-2021_02_08_430275 192 12 the the DT 10_1101-2021_02_08_430275 192 13 public public NN 10_1101-2021_02_08_430275 192 14 . . . 10_1101-2021_02_08_430275 193 1 We -PRON- PRP 10_1101-2021_02_08_430275 193 2 thank thank VBP 10_1101-2021_02_08_430275 193 3 Irene Irene NNP 10_1101-2021_02_08_430275 193 4 E. E. NNP 10_1101-2021_02_08_430275 193 5 Palmer Palmer NNP 10_1101-2021_02_08_430275 193 6 for for IN 10_1101-2021_02_08_430275 193 7 critical critical JJ 10_1101-2021_02_08_430275 193 8 review review NN 10_1101-2021_02_08_430275 193 9 and and CC 10_1101-2021_02_08_430275 193 10 466 466 CD 10_1101-2021_02_08_430275 193 11 thank thank VBP 10_1101-2021_02_08_430275 193 12 Nathan Nathan NNP 10_1101-2021_02_08_430275 193 13 Lynch Lynch NNP 10_1101-2021_02_08_430275 193 14 for for IN 10_1101-2021_02_08_430275 193 15 valuable valuable JJ 10_1101-2021_02_08_430275 193 16 comments comment NNS 10_1101-2021_02_08_430275 193 17 . . . 10_1101-2021_02_08_430275 194 1 The the DT 10_1101-2021_02_08_430275 194 2 manuscript manuscript NN 10_1101-2021_02_08_430275 194 3 was be VBD 10_1101-2021_02_08_430275 194 4 467 467 CD 10_1101-2021_02_08_430275 194 5 prepared prepare VBN 10_1101-2021_02_08_430275 194 6 using use VBG 10_1101-2021_02_08_430275 194 7 a a DT 10_1101-2021_02_08_430275 194 8 modified modify VBN 10_1101-2021_02_08_430275 194 9 version version NN 10_1101-2021_02_08_430275 194 10 of of IN 10_1101-2021_02_08_430275 194 11 the the DT 10_1101-2021_02_08_430275 194 12 PNAS PNAS NNP 10_1101-2021_02_08_430275 194 13 LATEX LATEX NNP 10_1101-2021_02_08_430275 194 14 template template NN 10_1101-2021_02_08_430275 194 15 . . . 10_1101-2021_02_08_430275 195 1 468 468 CD 10_1101-2021_02_08_430275 195 2 Bibliography Bibliography NNP 10_1101-2021_02_08_430275 195 3 469 469 CD 10_1101-2021_02_08_430275 195 4 1 1 CD 10_1101-2021_02_08_430275 195 5 . . . 10_1101-2021_02_08_430275 196 1 RW RW NNP 10_1101-2021_02_08_430275 196 2 Michelmore Michelmore NNP 10_1101-2021_02_08_430275 196 3 , , , 10_1101-2021_02_08_430275 196 4 I -PRON- PRP 10_1101-2021_02_08_430275 196 5 Paran Paran NNP 10_1101-2021_02_08_430275 196 6 , , , 10_1101-2021_02_08_430275 196 7 RV RV NNP 10_1101-2021_02_08_430275 196 8 Kesseli Kesseli NNP 10_1101-2021_02_08_430275 196 9 , , , 10_1101-2021_02_08_430275 196 10 Identification identification NN 10_1101-2021_02_08_430275 196 11 of of IN 10_1101-2021_02_08_430275 196 12 markers marker NNS 10_1101-2021_02_08_430275 196 13 linked link VBN 10_1101-2021_02_08_430275 196 14 to to IN 10_1101-2021_02_08_430275 196 15 disease disease NN 10_1101-2021_02_08_430275 196 16 - - HYPH 10_1101-2021_02_08_430275 196 17 resistance resistance NN 10_1101-2021_02_08_430275 196 18 470 470 CD 10_1101-2021_02_08_430275 196 19 genes gene NNS 10_1101-2021_02_08_430275 196 20 by by IN 10_1101-2021_02_08_430275 196 21 bulked bulk VBN 10_1101-2021_02_08_430275 196 22 segregant segregant NNP 10_1101-2021_02_08_430275 196 23 analysis analysis NNP 10_1101-2021_02_08_430275 196 24 : : : 10_1101-2021_02_08_430275 196 25 a a DT 10_1101-2021_02_08_430275 196 26 rapid rapid JJ 10_1101-2021_02_08_430275 196 27 method method NN 10_1101-2021_02_08_430275 196 28 to to TO 10_1101-2021_02_08_430275 196 29 detect detect VB 10_1101-2021_02_08_430275 196 30 markers marker NNS 10_1101-2021_02_08_430275 196 31 in in IN 10_1101-2021_02_08_430275 196 32 specific specific JJ 10_1101-2021_02_08_430275 196 33 genomic genomic NN 10_1101-2021_02_08_430275 196 34 re- re- XX 10_1101-2021_02_08_430275 196 35 471 471 CD 10_1101-2021_02_08_430275 196 36 gions gion NNS 10_1101-2021_02_08_430275 196 37 by by IN 10_1101-2021_02_08_430275 196 38 using use VBG 10_1101-2021_02_08_430275 196 39 segregating segregating NN 10_1101-2021_02_08_430275 196 40 populations population NNS 10_1101-2021_02_08_430275 196 41 . . . 10_1101-2021_02_08_430275 197 1 Proc Proc NNP 10_1101-2021_02_08_430275 197 2 . . . 10_1101-2021_02_08_430275 198 1 Natl Natl NNP 10_1101-2021_02_08_430275 198 2 . . . 10_1101-2021_02_08_430275 199 1 Acad Acad NNS 10_1101-2021_02_08_430275 199 2 . . . 10_1101-2021_02_08_430275 200 1 Sci Sci NNP 10_1101-2021_02_08_430275 200 2 . . . 10_1101-2021_02_08_430275 201 1 U.S.A. U.S.A. NNP 10_1101-2021_02_08_430275 201 2 88 88 CD 10_1101-2021_02_08_430275 201 3 , , , 10_1101-2021_02_08_430275 201 4 9828–9832 9828–9832 CD 10_1101-2021_02_08_430275 201 5 ( ( -LRB- 10_1101-2021_02_08_430275 201 6 1991 1991 CD 10_1101-2021_02_08_430275 201 7 ) ) -RRB- 10_1101-2021_02_08_430275 201 8 . . . 10_1101-2021_02_08_430275 202 1 472 472 CD 10_1101-2021_02_08_430275 202 2 2 2 CD 10_1101-2021_02_08_430275 202 3 . . . 10_1101-2021_02_08_430275 203 1 JJ JJ NNP 10_1101-2021_02_08_430275 203 2 Giovannoni Giovannoni NNP 10_1101-2021_02_08_430275 203 3 , , , 10_1101-2021_02_08_430275 203 4 RA RA NNP 10_1101-2021_02_08_430275 203 5 Wing Wing NNP 10_1101-2021_02_08_430275 203 6 , , , 10_1101-2021_02_08_430275 203 7 MW MW NNP 10_1101-2021_02_08_430275 203 8 Ganal Ganal NNP 10_1101-2021_02_08_430275 203 9 , , , 10_1101-2021_02_08_430275 203 10 SD SD NNP 10_1101-2021_02_08_430275 203 11 Tanksley Tanksley NNP 10_1101-2021_02_08_430275 203 12 , , , 10_1101-2021_02_08_430275 203 13 Isolation isolation NN 10_1101-2021_02_08_430275 203 14 of of IN 10_1101-2021_02_08_430275 203 15 molecular molecular JJ 10_1101-2021_02_08_430275 203 16 markers marker NNS 10_1101-2021_02_08_430275 203 17 from from IN 10_1101-2021_02_08_430275 203 18 spe- spe- RB 10_1101-2021_02_08_430275 203 19 473 473 CD 10_1101-2021_02_08_430275 203 20 cific cific JJ 10_1101-2021_02_08_430275 203 21 chromosomal chromosomal NN 10_1101-2021_02_08_430275 203 22 intervals interval NNS 10_1101-2021_02_08_430275 203 23 using use VBG 10_1101-2021_02_08_430275 203 24 DNA dna NN 10_1101-2021_02_08_430275 203 25 pools pool NNS 10_1101-2021_02_08_430275 203 26 from from IN 10_1101-2021_02_08_430275 203 27 existing exist VBG 10_1101-2021_02_08_430275 203 28 mapping mapping NN 10_1101-2021_02_08_430275 203 29 populations population NNS 10_1101-2021_02_08_430275 203 30 . . . 10_1101-2021_02_08_430275 204 1 Nucleic Nucleic NNP 10_1101-2021_02_08_430275 204 2 474 474 CD 10_1101-2021_02_08_430275 204 3 Acids Acids NNPS 10_1101-2021_02_08_430275 204 4 Res Res NNP 10_1101-2021_02_08_430275 204 5 19 19 CD 10_1101-2021_02_08_430275 204 6 , , , 10_1101-2021_02_08_430275 204 7 6553–6568 6553–6568 CD 10_1101-2021_02_08_430275 204 8 ( ( -LRB- 10_1101-2021_02_08_430275 204 9 1991 1991 CD 10_1101-2021_02_08_430275 204 10 ) ) -RRB- 10_1101-2021_02_08_430275 204 11 . . . 10_1101-2021_02_08_430275 205 1 475 475 CD 10_1101-2021_02_08_430275 205 2 3 3 CD 10_1101-2021_02_08_430275 205 3 . . . 10_1101-2021_02_08_430275 206 1 I -PRON- PRP 10_1101-2021_02_08_430275 206 2 Imerovski Imerovski NNP 10_1101-2021_02_08_430275 206 3 , , , 10_1101-2021_02_08_430275 206 4 et et NNP 10_1101-2021_02_08_430275 206 5 al al NNP 10_1101-2021_02_08_430275 206 6 . . NNP 10_1101-2021_02_08_430275 206 7 , , , 10_1101-2021_02_08_430275 206 8 BSA BSA NNP 10_1101-2021_02_08_430275 206 9 - - HYPH 10_1101-2021_02_08_430275 206 10 seq seq NN 10_1101-2021_02_08_430275 206 11 mapping mapping NN 10_1101-2021_02_08_430275 206 12 reveals reveal VBZ 10_1101-2021_02_08_430275 206 13 major major JJ 10_1101-2021_02_08_430275 206 14 QTL QTL NNP 10_1101-2021_02_08_430275 206 15 for for IN 10_1101-2021_02_08_430275 206 16 broomrape broomrape JJ 10_1101-2021_02_08_430275 206 17 resistance resistance NN 10_1101-2021_02_08_430275 206 18 in in IN 10_1101-2021_02_08_430275 206 19 four four CD 10_1101-2021_02_08_430275 206 20 476 476 CD 10_1101-2021_02_08_430275 206 21 sunflower sunflower NN 10_1101-2021_02_08_430275 206 22 lines line NNS 10_1101-2021_02_08_430275 206 23 . . . 10_1101-2021_02_08_430275 207 1 Mol Mol NNP 10_1101-2021_02_08_430275 207 2 Breed Breed NNP 10_1101-2021_02_08_430275 207 3 . . . 10_1101-2021_02_08_430275 208 1 39 39 CD 10_1101-2021_02_08_430275 208 2 , , , 10_1101-2021_02_08_430275 208 3 41 41 CD 10_1101-2021_02_08_430275 208 4 ( ( -LRB- 10_1101-2021_02_08_430275 208 5 2019 2019 CD 10_1101-2021_02_08_430275 208 6 ) ) -RRB- 10_1101-2021_02_08_430275 208 7 . . . 10_1101-2021_02_08_430275 209 1 477 477 CD 10_1101-2021_02_08_430275 209 2 4 4 CD 10_1101-2021_02_08_430275 209 3 . . . 10_1101-2021_02_08_430275 210 1 S S NNP 10_1101-2021_02_08_430275 210 2 Arikit Arikit NNP 10_1101-2021_02_08_430275 210 3 , , , 10_1101-2021_02_08_430275 210 4 et et NNP 10_1101-2021_02_08_430275 210 5 al al NNP 10_1101-2021_02_08_430275 210 6 . . NNP 10_1101-2021_02_08_430275 210 7 , , , 10_1101-2021_02_08_430275 210 8 QTL QTL NNP 10_1101-2021_02_08_430275 210 9 - - HYPH 10_1101-2021_02_08_430275 210 10 seq seq NN 10_1101-2021_02_08_430275 210 11 identifies identifie NNS 10_1101-2021_02_08_430275 210 12 cooked cook VBN 10_1101-2021_02_08_430275 210 13 grain grain NN 10_1101-2021_02_08_430275 210 14 elongation elongation NN 10_1101-2021_02_08_430275 210 15 QTLs qtl NNS 10_1101-2021_02_08_430275 210 16 near near IN 10_1101-2021_02_08_430275 210 17 soluble soluble JJ 10_1101-2021_02_08_430275 210 18 starch starch NN 10_1101-2021_02_08_430275 210 19 synthase synthase NN 10_1101-2021_02_08_430275 210 20 478 478 CD 10_1101-2021_02_08_430275 210 21 and and CC 10_1101-2021_02_08_430275 210 22 starch starch NN 10_1101-2021_02_08_430275 210 23 branching branching NN 10_1101-2021_02_08_430275 210 24 enzymes enzyme NNS 10_1101-2021_02_08_430275 210 25 in in IN 10_1101-2021_02_08_430275 210 26 rice rice NN 10_1101-2021_02_08_430275 210 27 ( ( -LRB- 10_1101-2021_02_08_430275 210 28 Oryza Oryza NNP 10_1101-2021_02_08_430275 210 29 sativa sativa NNP 10_1101-2021_02_08_430275 210 30 L. L. NNP 10_1101-2021_02_08_430275 210 31 ) ) -RRB- 10_1101-2021_02_08_430275 210 32 . . . 10_1101-2021_02_08_430275 211 1 Sci Sci NNP 10_1101-2021_02_08_430275 211 2 Rep Rep NNP 10_1101-2021_02_08_430275 211 3 9 9 CD 10_1101-2021_02_08_430275 211 4 , , , 10_1101-2021_02_08_430275 211 5 1–10 1–10 CD 10_1101-2021_02_08_430275 211 6 ( ( -LRB- 10_1101-2021_02_08_430275 211 7 2019 2019 CD 10_1101-2021_02_08_430275 211 8 ) ) -RRB- 10_1101-2021_02_08_430275 211 9 . . . 10_1101-2021_02_08_430275 212 1 479 479 CD 10_1101-2021_02_08_430275 212 2 5 5 CD 10_1101-2021_02_08_430275 212 3 . . . 10_1101-2021_02_08_430275 213 1 Q Q NNP 10_1101-2021_02_08_430275 213 2 Chen Chen NNP 10_1101-2021_02_08_430275 213 3 , , , 10_1101-2021_02_08_430275 213 4 et et NNP 10_1101-2021_02_08_430275 213 5 al al NNP 10_1101-2021_02_08_430275 213 6 . . NNP 10_1101-2021_02_08_430275 213 7 , , , 10_1101-2021_02_08_430275 213 8 Identification identification NN 10_1101-2021_02_08_430275 213 9 and and CC 10_1101-2021_02_08_430275 213 10 genetic genetic JJ 10_1101-2021_02_08_430275 213 11 mapping mapping NN 10_1101-2021_02_08_430275 213 12 for for IN 10_1101-2021_02_08_430275 213 13 rht rht NN 10_1101-2021_02_08_430275 213 14 - - HYPH 10_1101-2021_02_08_430275 213 15 DM DM NNP 10_1101-2021_02_08_430275 213 16 , , , 10_1101-2021_02_08_430275 213 17 a a DT 10_1101-2021_02_08_430275 213 18 dominant dominant JJ 10_1101-2021_02_08_430275 213 19 dwarfing dwarf VBG 10_1101-2021_02_08_430275 213 20 gene gene NN 10_1101-2021_02_08_430275 213 21 in in IN 10_1101-2021_02_08_430275 213 22 480 480 CD 10_1101-2021_02_08_430275 213 23 mutant mutant JJ 10_1101-2021_02_08_430275 213 24 semi semi JJ 10_1101-2021_02_08_430275 213 25 - - JJ 10_1101-2021_02_08_430275 213 26 dwarf dwarf JJ 10_1101-2021_02_08_430275 213 27 maize maize NN 10_1101-2021_02_08_430275 213 28 using use VBG 10_1101-2021_02_08_430275 213 29 QTL QTL NNP 10_1101-2021_02_08_430275 213 30 - - HYPH 10_1101-2021_02_08_430275 213 31 seq seq NN 10_1101-2021_02_08_430275 213 32 approach approach NN 10_1101-2021_02_08_430275 213 33 . . . 10_1101-2021_02_08_430275 214 1 Genes Genes NNP 10_1101-2021_02_08_430275 214 2 Genomics Genomics NNP 10_1101-2021_02_08_430275 214 3 40 40 CD 10_1101-2021_02_08_430275 214 4 , , , 10_1101-2021_02_08_430275 214 5 1091–1099 1091–1099 CD 10_1101-2021_02_08_430275 214 6 ( ( -LRB- 10_1101-2021_02_08_430275 214 7 2018 2018 CD 10_1101-2021_02_08_430275 214 8 ) ) -RRB- 10_1101-2021_02_08_430275 214 9 . . . 10_1101-2021_02_08_430275 215 1 481 481 CD 10_1101-2021_02_08_430275 215 2 6 6 CD 10_1101-2021_02_08_430275 215 3 . . . 10_1101-2021_02_08_430275 216 1 J J NNP 10_1101-2021_02_08_430275 216 2 Clevenger Clevenger NNP 10_1101-2021_02_08_430275 216 3 , , , 10_1101-2021_02_08_430275 216 4 et et NNP 10_1101-2021_02_08_430275 216 5 al al NNP 10_1101-2021_02_08_430275 216 6 . . NNP 10_1101-2021_02_08_430275 216 7 , , , 10_1101-2021_02_08_430275 216 8 Mapping Mapping NNP 10_1101-2021_02_08_430275 216 9 Late Late NNP 10_1101-2021_02_08_430275 216 10 Leaf Leaf NNP 10_1101-2021_02_08_430275 216 11 Spot Spot NNP 10_1101-2021_02_08_430275 216 12 Resistance Resistance NNP 10_1101-2021_02_08_430275 216 13 in in IN 10_1101-2021_02_08_430275 216 14 Peanut Peanut NNP 10_1101-2021_02_08_430275 216 15 ( ( -LRB- 10_1101-2021_02_08_430275 216 16 Arachis Arachis NNP 10_1101-2021_02_08_430275 216 17 hypogaea hypogaea XX 10_1101-2021_02_08_430275 216 18 ) ) -RRB- 10_1101-2021_02_08_430275 216 19 Using use VBG 10_1101-2021_02_08_430275 216 20 482 482 CD 10_1101-2021_02_08_430275 216 21 QTL qtl NN 10_1101-2021_02_08_430275 216 22 - - HYPH 10_1101-2021_02_08_430275 216 23 seq seq NN 10_1101-2021_02_08_430275 216 24 Reveals Reveals NNP 10_1101-2021_02_08_430275 216 25 Markers Markers NNPS 10_1101-2021_02_08_430275 216 26 for for IN 10_1101-2021_02_08_430275 216 27 Marker Marker NNP 10_1101-2021_02_08_430275 216 28 - - HYPH 10_1101-2021_02_08_430275 216 29 Assisted Assisted NNP 10_1101-2021_02_08_430275 216 30 Selection Selection NNP 10_1101-2021_02_08_430275 216 31 . . . 10_1101-2021_02_08_430275 217 1 Front Front NNP 10_1101-2021_02_08_430275 217 2 Plant Plant NNP 10_1101-2021_02_08_430275 217 3 Sci Sci NNP 10_1101-2021_02_08_430275 217 4 9 9 CD 10_1101-2021_02_08_430275 217 5 , , , 10_1101-2021_02_08_430275 217 6 83 83 CD 10_1101-2021_02_08_430275 217 7 ( ( -LRB- 10_1101-2021_02_08_430275 217 8 2018 2018 CD 10_1101-2021_02_08_430275 217 9 ) ) -RRB- 10_1101-2021_02_08_430275 217 10 . . . 10_1101-2021_02_08_430275 218 1 483 483 CD 10_1101-2021_02_08_430275 218 2 7 7 CD 10_1101-2021_02_08_430275 218 3 . . . 10_1101-2021_02_08_430275 219 1 F F NNP 10_1101-2021_02_08_430275 219 2 Duveau Duveau NNP 10_1101-2021_02_08_430275 219 3 , , , 10_1101-2021_02_08_430275 219 4 et et NNP 10_1101-2021_02_08_430275 219 5 al al NNP 10_1101-2021_02_08_430275 219 6 . . NNP 10_1101-2021_02_08_430275 219 7 , , , 10_1101-2021_02_08_430275 219 8 Mapping map VBG 10_1101-2021_02_08_430275 219 9 small small JJ 10_1101-2021_02_08_430275 219 10 effect effect NN 10_1101-2021_02_08_430275 219 11 mutations mutation NNS 10_1101-2021_02_08_430275 219 12 in in IN 10_1101-2021_02_08_430275 219 13 Saccharomyces saccharomyce NNS 10_1101-2021_02_08_430275 219 14 cerevisiae cerevisiae VBZ 10_1101-2021_02_08_430275 219 15 : : : 10_1101-2021_02_08_430275 219 16 impacts impact NNS 10_1101-2021_02_08_430275 219 17 of of IN 10_1101-2021_02_08_430275 219 18 484 484 CD 10_1101-2021_02_08_430275 219 19 experimental experimental JJ 10_1101-2021_02_08_430275 219 20 design design NN 10_1101-2021_02_08_430275 219 21 and and CC 10_1101-2021_02_08_430275 219 22 mutational mutational JJ 10_1101-2021_02_08_430275 219 23 properties property NNS 10_1101-2021_02_08_430275 219 24 . . . 10_1101-2021_02_08_430275 220 1 G3 G3 NNP 10_1101-2021_02_08_430275 220 2 ( ( -LRB- 10_1101-2021_02_08_430275 220 3 Bethesda Bethesda NNP 10_1101-2021_02_08_430275 220 4 ) ) -RRB- 10_1101-2021_02_08_430275 220 5 4 4 CD 10_1101-2021_02_08_430275 220 6 , , , 10_1101-2021_02_08_430275 220 7 1205–1216 1205–1216 CD 10_1101-2021_02_08_430275 220 8 ( ( -LRB- 10_1101-2021_02_08_430275 220 9 2014 2014 CD 10_1101-2021_02_08_430275 220 10 ) ) -RRB- 10_1101-2021_02_08_430275 220 11 . . . 10_1101-2021_02_08_430275 221 1 485 485 CD 10_1101-2021_02_08_430275 221 2 8 8 CD 10_1101-2021_02_08_430275 221 3 . . . 10_1101-2021_02_08_430275 222 1 J J NNP 10_1101-2021_02_08_430275 222 2 Zhang Zhang NNP 10_1101-2021_02_08_430275 222 3 , , , 10_1101-2021_02_08_430275 222 4 DR DR NNP 10_1101-2021_02_08_430275 222 5 Panthee Panthee NNP 10_1101-2021_02_08_430275 222 6 , , , 10_1101-2021_02_08_430275 222 7 PyBSASeq pybsaseq XX 10_1101-2021_02_08_430275 222 8 : : : 10_1101-2021_02_08_430275 222 9 a a DT 10_1101-2021_02_08_430275 222 10 simple simple JJ 10_1101-2021_02_08_430275 222 11 and and CC 10_1101-2021_02_08_430275 222 12 effective effective JJ 10_1101-2021_02_08_430275 222 13 algorithm algorithm NN 10_1101-2021_02_08_430275 222 14 for for IN 10_1101-2021_02_08_430275 222 15 bulked bulked NNP 10_1101-2021_02_08_430275 222 16 segregant segregant NNP 10_1101-2021_02_08_430275 222 17 486 486 CD 10_1101-2021_02_08_430275 222 18 analysis analysis NN 10_1101-2021_02_08_430275 222 19 with with IN 10_1101-2021_02_08_430275 222 20 whole whole RB 10_1101-2021_02_08_430275 222 21 - - HYPH 10_1101-2021_02_08_430275 222 22 genome genome RB 10_1101-2021_02_08_430275 222 23 sequencing sequencing NN 10_1101-2021_02_08_430275 222 24 data datum NNS 10_1101-2021_02_08_430275 222 25 . . . 10_1101-2021_02_08_430275 223 1 BMC BMC NNP 10_1101-2021_02_08_430275 223 2 Bioinforma Bioinforma NNP 10_1101-2021_02_08_430275 223 3 . . . 10_1101-2021_02_08_430275 224 1 21 21 CD 10_1101-2021_02_08_430275 224 2 , , , 10_1101-2021_02_08_430275 224 3 99 99 CD 10_1101-2021_02_08_430275 224 4 ( ( -LRB- 10_1101-2021_02_08_430275 224 5 2020 2020 CD 10_1101-2021_02_08_430275 224 6 ) ) -RRB- 10_1101-2021_02_08_430275 224 7 . . . 10_1101-2021_02_08_430275 225 1 487 487 CD 10_1101-2021_02_08_430275 225 2 9 9 CD 10_1101-2021_02_08_430275 225 3 . . . 10_1101-2021_02_08_430275 226 1 Z Z NNP 10_1101-2021_02_08_430275 226 2 Yang Yang NNP 10_1101-2021_02_08_430275 226 3 , , , 10_1101-2021_02_08_430275 226 4 et et NNP 10_1101-2021_02_08_430275 226 5 al al NNP 10_1101-2021_02_08_430275 226 6 . . NNP 10_1101-2021_02_08_430275 226 7 , , , 10_1101-2021_02_08_430275 226 8 Mapping Mapping NNP 10_1101-2021_02_08_430275 226 9 of of IN 10_1101-2021_02_08_430275 226 10 Quantitative Quantitative NNP 10_1101-2021_02_08_430275 226 11 Trait Trait NNP 10_1101-2021_02_08_430275 226 12 Loci Loci NNP 10_1101-2021_02_08_430275 226 13 Underlying Underlying NNP 10_1101-2021_02_08_430275 226 14 Cold Cold NNP 10_1101-2021_02_08_430275 226 15 Tolerance Tolerance NNP 10_1101-2021_02_08_430275 226 16 in in IN 10_1101-2021_02_08_430275 226 17 Rice Rice NNP 10_1101-2021_02_08_430275 226 18 Seedlings Seedlings NNP 10_1101-2021_02_08_430275 226 19 488 488 CD 10_1101-2021_02_08_430275 226 20 via via IN 10_1101-2021_02_08_430275 226 21 High High NNP 10_1101-2021_02_08_430275 226 22 - - HYPH 10_1101-2021_02_08_430275 226 23 Throughput throughput NN 10_1101-2021_02_08_430275 226 24 Sequencing sequencing NN 10_1101-2021_02_08_430275 226 25 of of IN 10_1101-2021_02_08_430275 226 26 Pooled pool VBN 10_1101-2021_02_08_430275 226 27 Extremes Extremes NNPS 10_1101-2021_02_08_430275 226 28 . . . 10_1101-2021_02_08_430275 227 1 PLOS PLOS NNP 10_1101-2021_02_08_430275 227 2 ONE one CD 10_1101-2021_02_08_430275 227 3 8 8 CD 10_1101-2021_02_08_430275 227 4 , , , 10_1101-2021_02_08_430275 227 5 e68433 e68433 NN 10_1101-2021_02_08_430275 227 6 ( ( -LRB- 10_1101-2021_02_08_430275 227 7 2013 2013 CD 10_1101-2021_02_08_430275 227 8 ) ) -RRB- 10_1101-2021_02_08_430275 227 9 . . . 10_1101-2021_02_08_430275 228 1 489 489 CD 10_1101-2021_02_08_430275 228 2 10 10 CD 10_1101-2021_02_08_430275 228 3 . . . 10_1101-2021_02_08_430275 229 1 H H NNP 10_1101-2021_02_08_430275 229 2 Takagi Takagi NNP 10_1101-2021_02_08_430275 229 3 , , , 10_1101-2021_02_08_430275 229 4 et et NNP 10_1101-2021_02_08_430275 229 5 al al NNP 10_1101-2021_02_08_430275 229 6 . . NNP 10_1101-2021_02_08_430275 229 7 , , , 10_1101-2021_02_08_430275 229 8 QTL QTL NNP 10_1101-2021_02_08_430275 229 9 - - HYPH 10_1101-2021_02_08_430275 229 10 seq seq NN 10_1101-2021_02_08_430275 229 11 : : : 10_1101-2021_02_08_430275 229 12 rapid rapid JJ 10_1101-2021_02_08_430275 229 13 mapping mapping NN 10_1101-2021_02_08_430275 229 14 of of IN 10_1101-2021_02_08_430275 229 15 quantitative quantitative JJ 10_1101-2021_02_08_430275 229 16 trait trait NNP 10_1101-2021_02_08_430275 229 17 loci loci NNP 10_1101-2021_02_08_430275 229 18 in in IN 10_1101-2021_02_08_430275 229 19 rice rice NN 10_1101-2021_02_08_430275 229 20 by by IN 10_1101-2021_02_08_430275 229 21 whole whole JJ 10_1101-2021_02_08_430275 229 22 genome genome NN 10_1101-2021_02_08_430275 229 23 490 490 CD 10_1101-2021_02_08_430275 229 24 resequencing resequencing NN 10_1101-2021_02_08_430275 229 25 of of IN 10_1101-2021_02_08_430275 229 26 DNA dna NN 10_1101-2021_02_08_430275 229 27 from from IN 10_1101-2021_02_08_430275 229 28 two two CD 10_1101-2021_02_08_430275 229 29 bulked bulked JJ 10_1101-2021_02_08_430275 229 30 populations population NNS 10_1101-2021_02_08_430275 229 31 . . . 10_1101-2021_02_08_430275 230 1 Plant Plant NNP 10_1101-2021_02_08_430275 230 2 J. J. NNP 10_1101-2021_02_08_430275 231 1 74 74 CD 10_1101-2021_02_08_430275 231 2 , , , 10_1101-2021_02_08_430275 231 3 174–183 174–183 CD 10_1101-2021_02_08_430275 231 4 ( ( -LRB- 10_1101-2021_02_08_430275 231 5 2013 2013 CD 10_1101-2021_02_08_430275 231 6 ) ) -RRB- 10_1101-2021_02_08_430275 231 7 . . . 10_1101-2021_02_08_430275 232 1 491 491 CD 10_1101-2021_02_08_430275 232 2 11 11 CD 10_1101-2021_02_08_430275 232 3 . . . 10_1101-2021_02_08_430275 233 1 PM PM NNP 10_1101-2021_02_08_430275 233 2 Magwene Magwene NNP 10_1101-2021_02_08_430275 233 3 , , , 10_1101-2021_02_08_430275 233 4 JH JH NNP 10_1101-2021_02_08_430275 233 5 Willis Willis NNP 10_1101-2021_02_08_430275 233 6 , , , 10_1101-2021_02_08_430275 233 7 JK JK NNP 10_1101-2021_02_08_430275 233 8 Kelly Kelly NNP 10_1101-2021_02_08_430275 233 9 , , , 10_1101-2021_02_08_430275 233 10 The the DT 10_1101-2021_02_08_430275 233 11 Statistics Statistics NNPS 10_1101-2021_02_08_430275 233 12 of of IN 10_1101-2021_02_08_430275 233 13 Bulk Bulk NNP 10_1101-2021_02_08_430275 233 14 Segregant Segregant NNP 10_1101-2021_02_08_430275 233 15 Analysis Analysis NNP 10_1101-2021_02_08_430275 233 16 Using use VBG 10_1101-2021_02_08_430275 233 17 Next Next NNP 10_1101-2021_02_08_430275 233 18 Gen- Gen- NNP 10_1101-2021_02_08_430275 233 19 492 492 CD 10_1101-2021_02_08_430275 233 20 eration eration NN 10_1101-2021_02_08_430275 233 21 Sequencing sequencing NN 10_1101-2021_02_08_430275 233 22 . . . 10_1101-2021_02_08_430275 234 1 PLOS PLOS NNP 10_1101-2021_02_08_430275 234 2 Comput comput NN 10_1101-2021_02_08_430275 234 3 . . . 10_1101-2021_02_08_430275 235 1 Biol Biol NNP 10_1101-2021_02_08_430275 235 2 . . . 10_1101-2021_02_08_430275 236 1 7 7 CD 10_1101-2021_02_08_430275 236 2 , , , 10_1101-2021_02_08_430275 236 3 e1002255 e1002255 NNP 10_1101-2021_02_08_430275 236 4 ( ( -LRB- 10_1101-2021_02_08_430275 236 5 2011 2011 CD 10_1101-2021_02_08_430275 236 6 ) ) -RRB- 10_1101-2021_02_08_430275 236 7 . . . 10_1101-2021_02_08_430275 237 1 493 493 CD 10_1101-2021_02_08_430275 237 2 12 12 CD 10_1101-2021_02_08_430275 237 3 . . . 10_1101-2021_02_08_430275 238 1 Z Z NNP 10_1101-2021_02_08_430275 238 2 Lahari Lahari NNP 10_1101-2021_02_08_430275 238 3 , , , 10_1101-2021_02_08_430275 238 4 et et NNP 10_1101-2021_02_08_430275 238 5 al al NNP 10_1101-2021_02_08_430275 238 6 . . NNP 10_1101-2021_02_08_430275 238 7 , , , 10_1101-2021_02_08_430275 238 8 QTL QTL NNP 10_1101-2021_02_08_430275 238 9 - - HYPH 10_1101-2021_02_08_430275 238 10 seq seq NN 10_1101-2021_02_08_430275 238 11 reveals reveal VBZ 10_1101-2021_02_08_430275 238 12 a a DT 10_1101-2021_02_08_430275 238 13 major major JJ 10_1101-2021_02_08_430275 238 14 root root NN 10_1101-2021_02_08_430275 238 15 - - HYPH 10_1101-2021_02_08_430275 238 16 knot knot NN 10_1101-2021_02_08_430275 238 17 nematode nematode NNP 10_1101-2021_02_08_430275 238 18 resistance resistance NNP 10_1101-2021_02_08_430275 238 19 locus locus NN 10_1101-2021_02_08_430275 238 20 on on IN 10_1101-2021_02_08_430275 238 21 chromo- chromo- NN 10_1101-2021_02_08_430275 238 22 494 494 CD 10_1101-2021_02_08_430275 238 23 some some DT 10_1101-2021_02_08_430275 238 24 11 11 CD 10_1101-2021_02_08_430275 238 25 in in IN 10_1101-2021_02_08_430275 238 26 rice rice NN 10_1101-2021_02_08_430275 238 27 ( ( -LRB- 10_1101-2021_02_08_430275 238 28 Oryza Oryza NNP 10_1101-2021_02_08_430275 238 29 sativa sativa NNP 10_1101-2021_02_08_430275 238 30 L. L. NNP 10_1101-2021_02_08_430275 238 31 ) ) -RRB- 10_1101-2021_02_08_430275 238 32 . . . 10_1101-2021_02_08_430275 239 1 Euphytica Euphytica NNP 10_1101-2021_02_08_430275 239 2 215 215 CD 10_1101-2021_02_08_430275 239 3 , , , 10_1101-2021_02_08_430275 239 4 117 117 CD 10_1101-2021_02_08_430275 239 5 ( ( -LRB- 10_1101-2021_02_08_430275 239 6 2019 2019 CD 10_1101-2021_02_08_430275 239 7 ) ) -RRB- 10_1101-2021_02_08_430275 239 8 . . . 10_1101-2021_02_08_430275 240 1 495 495 CD 10_1101-2021_02_08_430275 240 2 6 6 CD 10_1101-2021_02_08_430275 240 3 | | CD 10_1101-2021_02_08_430275 240 4 https://doi.org/10.1101/654137 https://doi.org/10.1101/654137 NN 10_1101-2021_02_08_430275 240 5 Zhang Zhang NNP 10_1101-2021_02_08_430275 240 6 et et NNP 10_1101-2021_02_08_430275 240 7 al al NNP 10_1101-2021_02_08_430275 240 8 . . . 10_1101-2021_02_08_430275 241 1 .CC .CC NFP 10_1101-2021_02_08_430275 241 2 - - : 10_1101-2021_02_08_430275 241 3 BY by IN 10_1101-2021_02_08_430275 241 4 - - HYPH 10_1101-2021_02_08_430275 241 5 NC NC NNP 10_1101-2021_02_08_430275 241 6 - - HYPH 10_1101-2021_02_08_430275 241 7 ND ND NNP 10_1101-2021_02_08_430275 241 8 4.0 4.0 CD 10_1101-2021_02_08_430275 241 9 International International NNP 10_1101-2021_02_08_430275 241 10 licenseavailable licenseavailable NN 10_1101-2021_02_08_430275 241 11 under under IN 10_1101-2021_02_08_430275 241 12 a a DT 10_1101-2021_02_08_430275 241 13 ( ( -LRB- 10_1101-2021_02_08_430275 241 14 which which WDT 10_1101-2021_02_08_430275 241 15 was be VBD 10_1101-2021_02_08_430275 241 16 not not RB 10_1101-2021_02_08_430275 241 17 certified certify VBN 10_1101-2021_02_08_430275 241 18 by by IN 10_1101-2021_02_08_430275 241 19 peer peer NN 10_1101-2021_02_08_430275 241 20 review review NN 10_1101-2021_02_08_430275 241 21 ) ) -RRB- 10_1101-2021_02_08_430275 241 22 is be VBZ 10_1101-2021_02_08_430275 241 23 the the DT 10_1101-2021_02_08_430275 241 24 author author NN 10_1101-2021_02_08_430275 241 25 / / SYM 10_1101-2021_02_08_430275 241 26 funder funder NN 10_1101-2021_02_08_430275 241 27 , , , 10_1101-2021_02_08_430275 241 28 who who WP 10_1101-2021_02_08_430275 241 29 has have VBZ 10_1101-2021_02_08_430275 241 30 granted grant VBN 10_1101-2021_02_08_430275 241 31 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 241 32 a a DT 10_1101-2021_02_08_430275 241 33 license license NN 10_1101-2021_02_08_430275 241 34 to to TO 10_1101-2021_02_08_430275 241 35 display display VB 10_1101-2021_02_08_430275 241 36 the the DT 10_1101-2021_02_08_430275 241 37 preprint preprint NN 10_1101-2021_02_08_430275 241 38 in in IN 10_1101-2021_02_08_430275 241 39 perpetuity perpetuity NN 10_1101-2021_02_08_430275 241 40 . . . 10_1101-2021_02_08_430275 242 1 It -PRON- PRP 10_1101-2021_02_08_430275 242 2 is be VBZ 10_1101-2021_02_08_430275 242 3 made make VBN 10_1101-2021_02_08_430275 242 4 The the DT 10_1101-2021_02_08_430275 242 5 copyright copyright NN 10_1101-2021_02_08_430275 242 6 holder holder NN 10_1101-2021_02_08_430275 242 7 for for IN 10_1101-2021_02_08_430275 242 8 this this DT 10_1101-2021_02_08_430275 242 9 preprintthis preprintthis NN 10_1101-2021_02_08_430275 242 10 version version NN 10_1101-2021_02_08_430275 242 11 posted post VBD 10_1101-2021_02_08_430275 242 12 February February NNP 10_1101-2021_02_08_430275 242 13 10 10 CD 10_1101-2021_02_08_430275 242 14 , , , 10_1101-2021_02_08_430275 242 15 2021 2021 CD 10_1101-2021_02_08_430275 242 16 . . . 10_1101-2021_02_08_430275 242 17 ; ; : 10_1101-2021_02_08_430275 242 18 https://doi.org/10.1101/2021.02.08.430275doi https://doi.org/10.1101/2021.02.08.430275doi ADD 10_1101-2021_02_08_430275 242 19 : : : 10_1101-2021_02_08_430275 242 20 bioRxiv biorxiv IN 10_1101-2021_02_08_430275 242 21 preprint preprint NN 10_1101-2021_02_08_430275 242 22 https://doi.org/10.1101/654137 https://doi.org/10.1101/654137 VBZ 10_1101-2021_02_08_430275 242 23 https://doi.org/10.1101/2021.02.08.430275 https://doi.org/10.1101/2021.02.08.430275 NNP 10_1101-2021_02_08_430275 242 24 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP