id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-286854-0s7oq0uv	Jin, Xi	Virus strain from a mild COVID-19 patient in Hangzhou represents a new trend in SARS-CoV-2 evolution potentially related to Furin cleavage site	2020-07-03		.txt	text/plain	6012	322	54	title: Virus strain from a mild COVID-19 patient in Hangzhou represents a new trend in SARS-CoV-2 evolution potentially related to Furin cleavage site The evolutionary pattern of SARS-CoV-2 towards FCS formation may result in its clinical symptom becoming closer to HKU-1 and OC43 caused mild flu-like symptoms, further showing its potential in differentiating into mild COVID-19 subtypes. Sequence alignment analysis indicated 38 mutation sites for ZJ01 compared with other SARS-CoV-2 family members ( Figure 2(A) ). Further comparative alignment analysis of GZ02 (SARS viral strain), Wuhan-Hu-1 (the earliest sequenced SARS-CoV-2), RaTG13, HKU9-1 (the potential ancestor of SARS and SARS-CoV-2), HKU-1 and OC43 showed that the variation of FCS sequence had certain regularity in coronavirus evolution ( Figure 4(B) ). We speculated that, despite the gene similarity between ZJ01 and Wuhan-Hu-1, the mutation near the FCS changed the protein structure conformation and surface electrostatic potential of ZJ01, which further influenced its binding capacity with Furin.	./cache/cord-286854-0s7oq0uv.txt	./txt/cord-286854-0s7oq0uv.txt