id sid tid token lemma pos cord-104239-xxlcdbqi 1 1 key key NN cord-104239-xxlcdbqi 1 2 : : : cord-104239-xxlcdbqi 2 1 cord-104239-xxlcdbqi cord-104239-xxlcdbqi NNP cord-104239-xxlcdbqi 2 2 authors author NNS cord-104239-xxlcdbqi 2 3 : : : cord-104239-xxlcdbqi 3 1 nan nan NNP cord-104239-xxlcdbqi 3 2 title title NN cord-104239-xxlcdbqi 3 3 : : : cord-104239-xxlcdbqi 4 1 The the DT cord-104239-xxlcdbqi 4 2 organization organization NN cord-104239-xxlcdbqi 4 3 of of IN cord-104239-xxlcdbqi 4 4 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 4 5 reticulum reticulum NN cord-104239-xxlcdbqi 4 6 export export NN cord-104239-xxlcdbqi 4 7 complexes complex NNS cord-104239-xxlcdbqi 4 8 date date NN cord-104239-xxlcdbqi 4 9 : : : cord-104239-xxlcdbqi 4 10 1996 1996 CD cord-104239-xxlcdbqi 4 11 - - SYM cord-104239-xxlcdbqi 4 12 10 10 CD cord-104239-xxlcdbqi 4 13 - - HYPH cord-104239-xxlcdbqi 4 14 01 01 CD cord-104239-xxlcdbqi 4 15 journal journal NN cord-104239-xxlcdbqi 4 16 : : : cord-104239-xxlcdbqi 4 17 J J NNP cord-104239-xxlcdbqi 4 18 Cell Cell NNP cord-104239-xxlcdbqi 4 19 Biol Biol NNP cord-104239-xxlcdbqi 4 20 DOI DOI NNP cord-104239-xxlcdbqi 4 21 : : : cord-104239-xxlcdbqi 5 1 nan nan NNP cord-104239-xxlcdbqi 5 2 sha sha NNP cord-104239-xxlcdbqi 5 3 : : : cord-104239-xxlcdbqi 5 4 077d5a9302762a66842c6bdf13912433954ca723 077d5a9302762a66842c6bdf13912433954ca723 CD cord-104239-xxlcdbqi 5 5 doc_id doc_id CD cord-104239-xxlcdbqi 5 6 : : : cord-104239-xxlcdbqi 6 1 104239 104239 CD cord-104239-xxlcdbqi 7 1 cord_uid cord_uid NN cord-104239-xxlcdbqi 7 2 : : : cord-104239-xxlcdbqi 8 1 xxlcdbqi xxlcdbqi NNP cord-104239-xxlcdbqi 8 2 Export Export NNP cord-104239-xxlcdbqi 8 3 of of IN cord-104239-xxlcdbqi 8 4 cargo cargo NN cord-104239-xxlcdbqi 8 5 from from IN cord-104239-xxlcdbqi 8 6 the the DT cord-104239-xxlcdbqi 8 7 ER ER NNP cord-104239-xxlcdbqi 8 8 occurs occur VBZ cord-104239-xxlcdbqi 8 9 through through IN cord-104239-xxlcdbqi 8 10 the the DT cord-104239-xxlcdbqi 8 11 formation formation NN cord-104239-xxlcdbqi 8 12 of of IN cord-104239-xxlcdbqi 8 13 60 60 CD cord-104239-xxlcdbqi 8 14 - - SYM cord-104239-xxlcdbqi 8 15 70 70 CD cord-104239-xxlcdbqi 8 16 nm nm NNP cord-104239-xxlcdbqi 8 17 COPII COPII NNP cord-104239-xxlcdbqi 8 18 - - HYPH cord-104239-xxlcdbqi 8 19 coated coat VBN cord-104239-xxlcdbqi 8 20 vesicular vesicular JJ cord-104239-xxlcdbqi 8 21 carriers carrier NNS cord-104239-xxlcdbqi 8 22 . . . cord-104239-xxlcdbqi 9 1 We -PRON- PRP cord-104239-xxlcdbqi 9 2 have have VBP cord-104239-xxlcdbqi 9 3 applied apply VBN cord-104239-xxlcdbqi 9 4 serial serial JJ cord-104239-xxlcdbqi 9 5 - - HYPH cord-104239-xxlcdbqi 9 6 thin thin JJ cord-104239-xxlcdbqi 9 7 sectioning sectioning NN cord-104239-xxlcdbqi 9 8 and and CC cord-104239-xxlcdbqi 9 9 stereology stereology NN cord-104239-xxlcdbqi 9 10 to to TO cord-104239-xxlcdbqi 9 11 quantitatively quantitatively RB cord-104239-xxlcdbqi 9 12 characterize characterize VB cord-104239-xxlcdbqi 9 13 the the DT cord-104239-xxlcdbqi 9 14 three three CD cord-104239-xxlcdbqi 9 15 - - HYPH cord-104239-xxlcdbqi 9 16 dimensional dimensional JJ cord-104239-xxlcdbqi 9 17 organization organization NN cord-104239-xxlcdbqi 9 18 of of IN cord-104239-xxlcdbqi 9 19 ER ER NNP cord-104239-xxlcdbqi 9 20 export export NN cord-104239-xxlcdbqi 9 21 sites site NNS cord-104239-xxlcdbqi 9 22 in in IN cord-104239-xxlcdbqi 9 23 vivo vivo NN cord-104239-xxlcdbqi 9 24 and and CC cord-104239-xxlcdbqi 9 25 in in IN cord-104239-xxlcdbqi 9 26 vitro vitro FW cord-104239-xxlcdbqi 9 27 . . . cord-104239-xxlcdbqi 10 1 We -PRON- PRP cord-104239-xxlcdbqi 10 2 find find VBP cord-104239-xxlcdbqi 10 3 that that IN cord-104239-xxlcdbqi 10 4 ER ER NNP cord-104239-xxlcdbqi 10 5 buds bud NNS cord-104239-xxlcdbqi 10 6 in in IN cord-104239-xxlcdbqi 10 7 vivo vivo NN cord-104239-xxlcdbqi 10 8 are be VBP cord-104239-xxlcdbqi 10 9 nonrandomly nonrandomly RB cord-104239-xxlcdbqi 10 10 distributed distribute VBN cord-104239-xxlcdbqi 10 11 , , , cord-104239-xxlcdbqi 10 12 being be VBG cord-104239-xxlcdbqi 10 13 concentrated concentrate VBN cord-104239-xxlcdbqi 10 14 in in IN cord-104239-xxlcdbqi 10 15 regional regional JJ cord-104239-xxlcdbqi 10 16 foci focus NNS cord-104239-xxlcdbqi 10 17 we -PRON- PRP cord-104239-xxlcdbqi 10 18 refer refer VBP cord-104239-xxlcdbqi 10 19 to to IN cord-104239-xxlcdbqi 10 20 as as IN cord-104239-xxlcdbqi 10 21 export export NN cord-104239-xxlcdbqi 10 22 complexes complex NNS cord-104239-xxlcdbqi 10 23 . . . cord-104239-xxlcdbqi 11 1 The the DT cord-104239-xxlcdbqi 11 2 basic basic JJ cord-104239-xxlcdbqi 11 3 organization organization NN cord-104239-xxlcdbqi 11 4 of of IN cord-104239-xxlcdbqi 11 5 an an DT cord-104239-xxlcdbqi 11 6 export export NN cord-104239-xxlcdbqi 11 7 complex complex NN cord-104239-xxlcdbqi 11 8 can can MD cord-104239-xxlcdbqi 11 9 be be VB cord-104239-xxlcdbqi 11 10 divided divide VBN cord-104239-xxlcdbqi 11 11 into into IN cord-104239-xxlcdbqi 11 12 an an DT cord-104239-xxlcdbqi 11 13 active active JJ cord-104239-xxlcdbqi 11 14 COPII COPII NNP cord-104239-xxlcdbqi 11 15 - - HYPH cord-104239-xxlcdbqi 11 16 containing contain VBG cord-104239-xxlcdbqi 11 17 budding bud VBG cord-104239-xxlcdbqi 11 18 zone zone NN cord-104239-xxlcdbqi 11 19 on on IN cord-104239-xxlcdbqi 11 20 a a DT cord-104239-xxlcdbqi 11 21 single single JJ cord-104239-xxlcdbqi 11 22 ER ER NNP cord-104239-xxlcdbqi 11 23 cisterna cisterna NN cord-104239-xxlcdbqi 11 24 , , , cord-104239-xxlcdbqi 11 25 which which WDT cord-104239-xxlcdbqi 11 26 is be VBZ cord-104239-xxlcdbqi 11 27 adjacent adjacent JJ cord-104239-xxlcdbqi 11 28 to to IN cord-104239-xxlcdbqi 11 29 budding budding JJ cord-104239-xxlcdbqi 11 30 zones zone NNS cord-104239-xxlcdbqi 11 31 found find VBN cord-104239-xxlcdbqi 11 32 on on IN cord-104239-xxlcdbqi 11 33 distantly distantly RB cord-104239-xxlcdbqi 11 34 connected connect VBN cord-104239-xxlcdbqi 11 35 ER ER NNP cord-104239-xxlcdbqi 11 36 cisternae cisternae NNP cord-104239-xxlcdbqi 11 37 . . . cord-104239-xxlcdbqi 12 1 These these DT cord-104239-xxlcdbqi 12 2 budding bud VBG cord-104239-xxlcdbqi 12 3 foci foci NN cord-104239-xxlcdbqi 12 4 surround surround NN cord-104239-xxlcdbqi 12 5 and and CC cord-104239-xxlcdbqi 12 6 face face VBP cord-104239-xxlcdbqi 12 7 a a DT cord-104239-xxlcdbqi 12 8 central central JJ cord-104239-xxlcdbqi 12 9 cluster cluster NN cord-104239-xxlcdbqi 12 10 of of IN cord-104239-xxlcdbqi 12 11 morphologically morphologically RB cord-104239-xxlcdbqi 12 12 independent independent JJ cord-104239-xxlcdbqi 12 13 vesicular vesicular JJ cord-104239-xxlcdbqi 12 14 - - HYPH cord-104239-xxlcdbqi 12 15 tubular tubular JJ cord-104239-xxlcdbqi 12 16 elements element NNS cord-104239-xxlcdbqi 12 17 that that WDT cord-104239-xxlcdbqi 12 18 contain contain VBP cord-104239-xxlcdbqi 12 19 COPI COPI NNP cord-104239-xxlcdbqi 12 20 coats coat NNS cord-104239-xxlcdbqi 12 21 involved involve VBN cord-104239-xxlcdbqi 12 22 in in IN cord-104239-xxlcdbqi 12 23 retrograde retrograde JJ cord-104239-xxlcdbqi 12 24 transport transport NN cord-104239-xxlcdbqi 12 25 . . . cord-104239-xxlcdbqi 13 1 Vesicles vesicle NNS cord-104239-xxlcdbqi 13 2 within within IN cord-104239-xxlcdbqi 13 3 these these DT cord-104239-xxlcdbqi 13 4 export export NN cord-104239-xxlcdbqi 13 5 complexes complex NNS cord-104239-xxlcdbqi 13 6 contain contain VBP cord-104239-xxlcdbqi 13 7 concentrated concentrated JJ cord-104239-xxlcdbqi 13 8 cargo cargo NN cord-104239-xxlcdbqi 13 9 molecules molecule NNS cord-104239-xxlcdbqi 13 10 . . . cord-104239-xxlcdbqi 14 1 The the DT cord-104239-xxlcdbqi 14 2 structure structure NN cord-104239-xxlcdbqi 14 3 of of IN cord-104239-xxlcdbqi 14 4 vesicular vesicular JJ cord-104239-xxlcdbqi 14 5 - - HYPH cord-104239-xxlcdbqi 14 6 tubular tubular JJ cord-104239-xxlcdbqi 14 7 clusters cluster NNS cord-104239-xxlcdbqi 14 8 in in IN cord-104239-xxlcdbqi 14 9 export export NN cord-104239-xxlcdbqi 14 10 complexes complex NNS cord-104239-xxlcdbqi 14 11 is be VBZ cord-104239-xxlcdbqi 14 12 particularly particularly RB cord-104239-xxlcdbqi 14 13 striking strike VBG cord-104239-xxlcdbqi 14 14 in in IN cord-104239-xxlcdbqi 14 15 replicas replica NNS cord-104239-xxlcdbqi 14 16 generated generate VBN cord-104239-xxlcdbqi 14 17 using use VBG cord-104239-xxlcdbqi 14 18 a a DT cord-104239-xxlcdbqi 14 19 quick quick JJ cord-104239-xxlcdbqi 14 20 - - HYPH cord-104239-xxlcdbqi 14 21 freeze freeze JJ cord-104239-xxlcdbqi 14 22 , , , cord-104239-xxlcdbqi 14 23 deep deep JJ cord-104239-xxlcdbqi 14 24 - - HYPH cord-104239-xxlcdbqi 14 25 etch etch NN cord-104239-xxlcdbqi 14 26 approach approach NN cord-104239-xxlcdbqi 14 27 to to TO cord-104239-xxlcdbqi 14 28 visualize visualize VB cord-104239-xxlcdbqi 14 29 for for IN cord-104239-xxlcdbqi 14 30 the the DT cord-104239-xxlcdbqi 14 31 first first JJ cord-104239-xxlcdbqi 14 32 time time NN cord-104239-xxlcdbqi 14 33 their -PRON- PRP$ cord-104239-xxlcdbqi 14 34 three three CD cord-104239-xxlcdbqi 14 35 - - HYPH cord-104239-xxlcdbqi 14 36 dimensional dimensional JJ cord-104239-xxlcdbqi 14 37 organization organization NN cord-104239-xxlcdbqi 14 38 and and CC cord-104239-xxlcdbqi 14 39 cargo cargo NN cord-104239-xxlcdbqi 14 40 composition composition NN cord-104239-xxlcdbqi 14 41 . . . cord-104239-xxlcdbqi 15 1 We -PRON- PRP cord-104239-xxlcdbqi 15 2 conclude conclude VBP cord-104239-xxlcdbqi 15 3 that that IN cord-104239-xxlcdbqi 15 4 budding bud VBG cord-104239-xxlcdbqi 15 5 from from IN cord-104239-xxlcdbqi 15 6 the the DT cord-104239-xxlcdbqi 15 7 ER ER NNP cord-104239-xxlcdbqi 15 8 through through IN cord-104239-xxlcdbqi 15 9 recruitment recruitment NN cord-104239-xxlcdbqi 15 10 of of IN cord-104239-xxlcdbqi 15 11 COPII COPII NNP cord-104239-xxlcdbqi 15 12 is be VBZ cord-104239-xxlcdbqi 15 13 confined confine VBN cord-104239-xxlcdbqi 15 14 to to IN cord-104239-xxlcdbqi 15 15 highly highly RB cord-104239-xxlcdbqi 15 16 specialized specialized JJ cord-104239-xxlcdbqi 15 17 export export NN cord-104239-xxlcdbqi 15 18 complexes complex NNS cord-104239-xxlcdbqi 15 19 that that WDT cord-104239-xxlcdbqi 15 20 topologically topologically RB cord-104239-xxlcdbqi 15 21 restrict restrict VBP cord-104239-xxlcdbqi 15 22 anterograde anterograde JJ cord-104239-xxlcdbqi 15 23 transport transport NN cord-104239-xxlcdbqi 15 24 to to IN cord-104239-xxlcdbqi 15 25 regional regional JJ cord-104239-xxlcdbqi 15 26 foci focus NNS cord-104239-xxlcdbqi 15 27 to to TO cord-104239-xxlcdbqi 15 28 facilitate facilitate VB cord-104239-xxlcdbqi 15 29 efficient efficient JJ cord-104239-xxlcdbqi 15 30 coupling coupling NN cord-104239-xxlcdbqi 15 31 to to IN cord-104239-xxlcdbqi 15 32 retrograde retrograde JJ cord-104239-xxlcdbqi 15 33 recycling recycling NN cord-104239-xxlcdbqi 15 34 by by IN cord-104239-xxlcdbqi 15 35 COPI COPI NNP cord-104239-xxlcdbqi 15 36 . . . cord-104239-xxlcdbqi 16 1 Abstract Abstract NNP cord-104239-xxlcdbqi 16 2 . . . cord-104239-xxlcdbqi 17 1 Export export NN cord-104239-xxlcdbqi 17 2 of of IN cord-104239-xxlcdbqi 17 3 cargo cargo NN cord-104239-xxlcdbqi 17 4 from from IN cord-104239-xxlcdbqi 17 5 the the DT cord-104239-xxlcdbqi 17 6 ER ER NNP cord-104239-xxlcdbqi 17 7 occurs occur VBZ cord-104239-xxlcdbqi 17 8 through through IN cord-104239-xxlcdbqi 17 9 the the DT cord-104239-xxlcdbqi 17 10 formation formation NN cord-104239-xxlcdbqi 17 11 of of IN cord-104239-xxlcdbqi 17 12 60 60 CD cord-104239-xxlcdbqi 17 13 - - HYPH cord-104239-xxlcdbqi 17 14 70-nm 70-nm CD cord-104239-xxlcdbqi 17 15 COPII COPII NNP cord-104239-xxlcdbqi 17 16 - - HYPH cord-104239-xxlcdbqi 17 17 coated coat VBN cord-104239-xxlcdbqi 17 18 vesicular vesicular JJ cord-104239-xxlcdbqi 17 19 carriers carrier NNS cord-104239-xxlcdbqi 17 20 . . . cord-104239-xxlcdbqi 18 1 We -PRON- PRP cord-104239-xxlcdbqi 18 2 have have VBP cord-104239-xxlcdbqi 18 3 applied apply VBN cord-104239-xxlcdbqi 18 4 serial serial JJ cord-104239-xxlcdbqi 18 5 - - HYPH cord-104239-xxlcdbqi 18 6 thin thin JJ cord-104239-xxlcdbqi 18 7 sectioning sectioning NN cord-104239-xxlcdbqi 18 8 and and CC cord-104239-xxlcdbqi 18 9 stereology stereology NN cord-104239-xxlcdbqi 18 10 to to TO cord-104239-xxlcdbqi 18 11 quantitatively quantitatively RB cord-104239-xxlcdbqi 18 12 characterize characterize VB cord-104239-xxlcdbqi 18 13 the the DT cord-104239-xxlcdbqi 18 14 three three CD cord-104239-xxlcdbqi 18 15 - - HYPH cord-104239-xxlcdbqi 18 16 dimensional dimensional JJ cord-104239-xxlcdbqi 18 17 organization organization NN cord-104239-xxlcdbqi 18 18 of of IN cord-104239-xxlcdbqi 18 19 ER ER NNP cord-104239-xxlcdbqi 18 20 export export NN cord-104239-xxlcdbqi 18 21 sites site NNS cord-104239-xxlcdbqi 18 22 in in IN cord-104239-xxlcdbqi 18 23 vivo vivo NN cord-104239-xxlcdbqi 18 24 and and CC cord-104239-xxlcdbqi 18 25 in in IN cord-104239-xxlcdbqi 18 26 vitro vitro FW cord-104239-xxlcdbqi 18 27 . . . cord-104239-xxlcdbqi 19 1 We -PRON- PRP cord-104239-xxlcdbqi 19 2 find find VBP cord-104239-xxlcdbqi 19 3 that that IN cord-104239-xxlcdbqi 19 4 ER ER NNP cord-104239-xxlcdbqi 19 5 buds bud NNS cord-104239-xxlcdbqi 19 6 in in IN cord-104239-xxlcdbqi 19 7 vivo vivo NN cord-104239-xxlcdbqi 19 8 are be VBP cord-104239-xxlcdbqi 19 9 nonrandomly nonrandomly RB cord-104239-xxlcdbqi 19 10 distributed distribute VBN cord-104239-xxlcdbqi 19 11 , , , cord-104239-xxlcdbqi 19 12 being be VBG cord-104239-xxlcdbqi 19 13 concentrated concentrate VBN cord-104239-xxlcdbqi 19 14 in in IN cord-104239-xxlcdbqi 19 15 regional regional JJ cord-104239-xxlcdbqi 19 16 foci focus NNS cord-104239-xxlcdbqi 19 17 we -PRON- PRP cord-104239-xxlcdbqi 19 18 refer refer VBP cord-104239-xxlcdbqi 19 19 to to IN cord-104239-xxlcdbqi 19 20 as as IN cord-104239-xxlcdbqi 19 21 export export NN cord-104239-xxlcdbqi 19 22 complexes complex NNS cord-104239-xxlcdbqi 19 23 . . . cord-104239-xxlcdbqi 20 1 The the DT cord-104239-xxlcdbqi 20 2 basic basic JJ cord-104239-xxlcdbqi 20 3 organization organization NN cord-104239-xxlcdbqi 20 4 of of IN cord-104239-xxlcdbqi 20 5 an an DT cord-104239-xxlcdbqi 20 6 export export NN cord-104239-xxlcdbqi 20 7 complex complex NN cord-104239-xxlcdbqi 20 8 can can MD cord-104239-xxlcdbqi 20 9 be be VB cord-104239-xxlcdbqi 20 10 divided divide VBN cord-104239-xxlcdbqi 20 11 into into IN cord-104239-xxlcdbqi 20 12 an an DT cord-104239-xxlcdbqi 20 13 active active JJ cord-104239-xxlcdbqi 20 14 COPIIcontaining copiicontaining NN cord-104239-xxlcdbqi 20 15 budding bud VBG cord-104239-xxlcdbqi 20 16 zone zone NN cord-104239-xxlcdbqi 20 17 on on IN cord-104239-xxlcdbqi 20 18 a a DT cord-104239-xxlcdbqi 20 19 single single JJ cord-104239-xxlcdbqi 20 20 ER ER NNP cord-104239-xxlcdbqi 20 21 cisterna cisterna NN cord-104239-xxlcdbqi 20 22 , , , cord-104239-xxlcdbqi 20 23 which which WDT cord-104239-xxlcdbqi 20 24 is be VBZ cord-104239-xxlcdbqi 20 25 adjacent adjacent JJ cord-104239-xxlcdbqi 20 26 to to IN cord-104239-xxlcdbqi 20 27 budding budding JJ cord-104239-xxlcdbqi 20 28 zones zone NNS cord-104239-xxlcdbqi 20 29 found find VBN cord-104239-xxlcdbqi 20 30 on on IN cord-104239-xxlcdbqi 20 31 distantly distantly RB cord-104239-xxlcdbqi 20 32 connected connect VBN cord-104239-xxlcdbqi 20 33 ER ER NNP cord-104239-xxlcdbqi 20 34 cisternae cisternae NNP cord-104239-xxlcdbqi 20 35 . . . cord-104239-xxlcdbqi 21 1 These these DT cord-104239-xxlcdbqi 21 2 budding bud VBG cord-104239-xxlcdbqi 21 3 foci foci NN cord-104239-xxlcdbqi 21 4 surround surround NN cord-104239-xxlcdbqi 21 5 and and CC cord-104239-xxlcdbqi 21 6 face face VBP cord-104239-xxlcdbqi 21 7 a a DT cord-104239-xxlcdbqi 21 8 central central JJ cord-104239-xxlcdbqi 21 9 cluster cluster NN cord-104239-xxlcdbqi 21 10 of of IN cord-104239-xxlcdbqi 21 11 morphologically morphologically RB cord-104239-xxlcdbqi 21 12 independent independent JJ cord-104239-xxlcdbqi 21 13 vesicular vesicular JJ cord-104239-xxlcdbqi 21 14 - - HYPH cord-104239-xxlcdbqi 21 15 tubular tubular JJ cord-104239-xxlcdbqi 21 16 elements element NNS cord-104239-xxlcdbqi 21 17 that that WDT cord-104239-xxlcdbqi 21 18 contain contain VBP cord-104239-xxlcdbqi 21 19 COPI COPI NNP cord-104239-xxlcdbqi 21 20 coats coat NNS cord-104239-xxlcdbqi 21 21 involved involve VBN cord-104239-xxlcdbqi 21 22 in in IN cord-104239-xxlcdbqi 21 23 retrograde retrograde JJ cord-104239-xxlcdbqi 21 24 transport transport NN cord-104239-xxlcdbqi 21 25 . . . cord-104239-xxlcdbqi 22 1 Vesicles vesicle NNS cord-104239-xxlcdbqi 22 2 within within IN cord-104239-xxlcdbqi 22 3 these these DT cord-104239-xxlcdbqi 22 4 export export NN cord-104239-xxlcdbqi 22 5 complexes complex NNS cord-104239-xxlcdbqi 22 6 contain contain VBP cord-104239-xxlcdbqi 22 7 concentrated concentrated JJ cord-104239-xxlcdbqi 22 8 cargo cargo NN cord-104239-xxlcdbqi 22 9 molecules molecule NNS cord-104239-xxlcdbqi 22 10 . . . cord-104239-xxlcdbqi 23 1 The the DT cord-104239-xxlcdbqi 23 2 structure structure NN cord-104239-xxlcdbqi 23 3 of of IN cord-104239-xxlcdbqi 23 4 vesicular vesicular JJ cord-104239-xxlcdbqi 23 5 - - HYPH cord-104239-xxlcdbqi 23 6 tubular tubular JJ cord-104239-xxlcdbqi 23 7 clusters cluster NNS cord-104239-xxlcdbqi 23 8 in in IN cord-104239-xxlcdbqi 23 9 export export NN cord-104239-xxlcdbqi 23 10 complexes complex NNS cord-104239-xxlcdbqi 23 11 is be VBZ cord-104239-xxlcdbqi 23 12 particularly particularly RB cord-104239-xxlcdbqi 23 13 striking strike VBG cord-104239-xxlcdbqi 23 14 in in IN cord-104239-xxlcdbqi 23 15 replicas replica NNS cord-104239-xxlcdbqi 23 16 generated generate VBN cord-104239-xxlcdbqi 23 17 using use VBG cord-104239-xxlcdbqi 23 18 a a DT cord-104239-xxlcdbqi 23 19 quick quick JJ cord-104239-xxlcdbqi 23 20 - - HYPH cord-104239-xxlcdbqi 23 21 freeze freeze JJ cord-104239-xxlcdbqi 23 22 , , , cord-104239-xxlcdbqi 23 23 deep deep JJ cord-104239-xxlcdbqi 23 24 - - HYPH cord-104239-xxlcdbqi 23 25 etch etch NN cord-104239-xxlcdbqi 23 26 approach approach NN cord-104239-xxlcdbqi 23 27 to to TO cord-104239-xxlcdbqi 23 28 visualize visualize VB cord-104239-xxlcdbqi 23 29 for for IN cord-104239-xxlcdbqi 23 30 the the DT cord-104239-xxlcdbqi 23 31 first first JJ cord-104239-xxlcdbqi 23 32 time time NN cord-104239-xxlcdbqi 23 33 their -PRON- PRP$ cord-104239-xxlcdbqi 23 34 three three CD cord-104239-xxlcdbqi 23 35 - - HYPH cord-104239-xxlcdbqi 23 36 dimensional dimensional JJ cord-104239-xxlcdbqi 23 37 organization organization NN cord-104239-xxlcdbqi 23 38 and and CC cord-104239-xxlcdbqi 23 39 cargo cargo NN cord-104239-xxlcdbqi 23 40 composition composition NN cord-104239-xxlcdbqi 23 41 . . . cord-104239-xxlcdbqi 24 1 We -PRON- PRP cord-104239-xxlcdbqi 24 2 conclude conclude VBP cord-104239-xxlcdbqi 24 3 that that IN cord-104239-xxlcdbqi 24 4 budding bud VBG cord-104239-xxlcdbqi 24 5 from from IN cord-104239-xxlcdbqi 24 6 the the DT cord-104239-xxlcdbqi 24 7 ER ER NNP cord-104239-xxlcdbqi 24 8 through through IN cord-104239-xxlcdbqi 24 9 recruitment recruitment NN cord-104239-xxlcdbqi 24 10 of of IN cord-104239-xxlcdbqi 24 11 COPI COPI NNP cord-104239-xxlcdbqi 24 12 ! ! . cord-104239-xxlcdbqi 25 1 is be VBZ cord-104239-xxlcdbqi 25 2 confined confine VBN cord-104239-xxlcdbqi 25 3 to to IN cord-104239-xxlcdbqi 25 4 highly highly RB cord-104239-xxlcdbqi 25 5 specialized specialized JJ cord-104239-xxlcdbqi 25 6 export export NN cord-104239-xxlcdbqi 25 7 complexes complex NNS cord-104239-xxlcdbqi 25 8 that that WDT cord-104239-xxlcdbqi 25 9 topologically topologically RB cord-104239-xxlcdbqi 25 10 restrict restrict VBP cord-104239-xxlcdbqi 25 11 anterograde anterograde JJ cord-104239-xxlcdbqi 25 12 transport transport NN cord-104239-xxlcdbqi 25 13 to to IN cord-104239-xxlcdbqi 25 14 regional regional JJ cord-104239-xxlcdbqi 25 15 foci focus NNS cord-104239-xxlcdbqi 25 16 to to TO cord-104239-xxlcdbqi 25 17 facilitate facilitate VB cord-104239-xxlcdbqi 25 18 efficient efficient JJ cord-104239-xxlcdbqi 25 19 coupling coupling NN cord-104239-xxlcdbqi 25 20 to to IN cord-104239-xxlcdbqi 25 21 retrograde retrograde JJ cord-104239-xxlcdbqi 25 22 recycling recycling NN cord-104239-xxlcdbqi 25 23 by by IN cord-104239-xxlcdbqi 25 24 COPI COPI NNP cord-104239-xxlcdbqi 25 25 . . . cord-104239-xxlcdbqi 26 1 XPORT XPORT NNP cord-104239-xxlcdbqi 26 2 of of IN cord-104239-xxlcdbqi 26 3 protein protein NN cord-104239-xxlcdbqi 26 4 from from IN cord-104239-xxlcdbqi 26 5 the the DT cord-104239-xxlcdbqi 26 6 ER ER NNP cord-104239-xxlcdbqi 26 7 is be VBZ cord-104239-xxlcdbqi 26 8 the the DT cord-104239-xxlcdbqi 26 9 first first JJ cord-104239-xxlcdbqi 26 10 step step NN cord-104239-xxlcdbqi 26 11 in in IN cord-104239-xxlcdbqi 26 12 the the DT cord-104239-xxlcdbqi 26 13 vectorial vectorial JJ cord-104239-xxlcdbqi 26 14 movement movement NN cord-104239-xxlcdbqi 26 15 of of IN cord-104239-xxlcdbqi 26 16 cargo cargo NN cord-104239-xxlcdbqi 26 17 through through IN cord-104239-xxlcdbqi 26 18 compartments compartment NNS cord-104239-xxlcdbqi 26 19 of of IN cord-104239-xxlcdbqi 26 20 the the DT cord-104239-xxlcdbqi 26 21 secretory secretory JJ cord-104239-xxlcdbqi 26 22 pathway pathway NN cord-104239-xxlcdbqi 26 23 of of IN cord-104239-xxlcdbqi 26 24 eukaryotic eukaryotic JJ cord-104239-xxlcdbqi 26 25 cells cell NNS cord-104239-xxlcdbqi 26 26 . . . cord-104239-xxlcdbqi 27 1 Pioneering pioneering JJ cord-104239-xxlcdbqi 27 2 studies study NNS cord-104239-xxlcdbqi 27 3 by by IN cord-104239-xxlcdbqi 27 4 Palade Palade NNP cord-104239-xxlcdbqi 27 5 ( ( -LRB- cord-104239-xxlcdbqi 27 6 1975 1975 CD cord-104239-xxlcdbqi 27 7 ) ) -RRB- cord-104239-xxlcdbqi 27 8 established establish VBD cord-104239-xxlcdbqi 27 9 that that IN cord-104239-xxlcdbqi 27 10 the the DT cord-104239-xxlcdbqi 27 11 site site NN cord-104239-xxlcdbqi 27 12 of of IN cord-104239-xxlcdbqi 27 13 exit exit NN cord-104239-xxlcdbqi 27 14 from from IN cord-104239-xxlcdbqi 27 15 the the DT cord-104239-xxlcdbqi 27 16 RER RER NNP cord-104239-xxlcdbqi 27 17 in in IN cord-104239-xxlcdbqi 27 18 pancreatic pancreatic JJ cord-104239-xxlcdbqi 27 19 acinar acinar NN cord-104239-xxlcdbqi 27 20 cells cell NNS cord-104239-xxlcdbqi 27 21 is be VBZ cord-104239-xxlcdbqi 27 22 through through IN cord-104239-xxlcdbqi 27 23 transitional transitional JJ cord-104239-xxlcdbqi 27 24 elements element NNS cord-104239-xxlcdbqi 27 25 , , , cord-104239-xxlcdbqi 27 26 a a DT cord-104239-xxlcdbqi 27 27 region region NN cord-104239-xxlcdbqi 27 28 of of IN cord-104239-xxlcdbqi 27 29 partly partly RB cord-104239-xxlcdbqi 27 30 rough rough JJ cord-104239-xxlcdbqi 27 31 , , , cord-104239-xxlcdbqi 27 32 partly partly RB cord-104239-xxlcdbqi 27 33 smooth smooth JJ cord-104239-xxlcdbqi 27 34 tubular tubular JJ cord-104239-xxlcdbqi 27 35 ER er NN cord-104239-xxlcdbqi 27 36 juxtaposed juxtapose VBN cord-104239-xxlcdbqi 27 37 to to IN cord-104239-xxlcdbqi 27 38 the the DT cord-104239-xxlcdbqi 27 39 cis cis NNP cord-104239-xxlcdbqi 27 40 face face NN cord-104239-xxlcdbqi 27 41 of of IN cord-104239-xxlcdbqi 27 42 the the DT cord-104239-xxlcdbqi 27 43 Golgi Golgi NNP cord-104239-xxlcdbqi 27 44 stack stack NN cord-104239-xxlcdbqi 27 45 . . . cord-104239-xxlcdbqi 28 1 Morphologically Morphologically NNP cord-104239-xxlcdbqi 28 2 , , , cord-104239-xxlcdbqi 28 3 ER ER NNP cord-104239-xxlcdbqi 28 4 - - HYPH cord-104239-xxlcdbqi 28 5 derived derive VBN cord-104239-xxlcdbqi 28 6 vesicles vesicle NNS cord-104239-xxlcdbqi 28 7 are be VBP cord-104239-xxlcdbqi 28 8 60 60 CD cord-104239-xxlcdbqi 28 9 - - SYM cord-104239-xxlcdbqi 28 10 70 70 CD cord-104239-xxlcdbqi 28 11 nm nm NN cord-104239-xxlcdbqi 28 12 in in IN cord-104239-xxlcdbqi 28 13 diameter diameter NN cord-104239-xxlcdbqi 28 14 and and CC cord-104239-xxlcdbqi 28 15 contain contain VB cord-104239-xxlcdbqi 28 16 an an DT cord-104239-xxlcdbqi 28 17 electron electron NN cord-104239-xxlcdbqi 28 18 - - HYPH cord-104239-xxlcdbqi 28 19 dense dense JJ cord-104239-xxlcdbqi 28 20 coat coat NN cord-104239-xxlcdbqi 28 21 when when WRB cord-104239-xxlcdbqi 28 22 viewed view VBN cord-104239-xxlcdbqi 28 23 using use VBG cord-104239-xxlcdbqi 28 24 transmission transmission NN cord-104239-xxlcdbqi 28 25 electron electron NN cord-104239-xxlcdbqi 28 26 microscopy microscopy NN cord-104239-xxlcdbqi 28 27 ( ( -LRB- cord-104239-xxlcdbqi 28 28 Ziegel Ziegel NNP cord-104239-xxlcdbqi 28 29 and and CC cord-104239-xxlcdbqi 28 30 Dalton Dalton NNP cord-104239-xxlcdbqi 28 31 , , , cord-104239-xxlcdbqi 28 32 1962 1962 CD cord-104239-xxlcdbqi 28 33 ) ) -RRB- cord-104239-xxlcdbqi 28 34 . . . cord-104239-xxlcdbqi 29 1 This this DT cord-104239-xxlcdbqi 29 2 characteristic characteristic JJ cord-104239-xxlcdbqi 29 3 coat coat NN cord-104239-xxlcdbqi 29 4 contains contain VBZ cord-104239-xxlcdbqi 29 5 COPII COPII NNP cord-104239-xxlcdbqi 29 6 components component NNS cord-104239-xxlcdbqi 29 7 that that WDT cord-104239-xxlcdbqi 29 8 are be VBP cord-104239-xxlcdbqi 29 9 assembled assemble VBN cord-104239-xxlcdbqi 29 10 in in IN cord-104239-xxlcdbqi 29 11 response response NN cord-104239-xxlcdbqi 29 12 to to IN cord-104239-xxlcdbqi 29 13 the the DT cord-104239-xxlcdbqi 29 14 activation activation NN cord-104239-xxlcdbqi 29 15 of of IN cord-104239-xxlcdbqi 29 16 the the DT cord-104239-xxlcdbqi 29 17 Sarl Sarl NNP cord-104239-xxlcdbqi 29 18 GTPase GTPase NNP cord-104239-xxlcdbqi 29 19 , , , cord-104239-xxlcdbqi 29 20 a a DT cord-104239-xxlcdbqi 29 21 machinery machinery NN cord-104239-xxlcdbqi 29 22 now now RB cord-104239-xxlcdbqi 29 23 recognized recognize VBN cord-104239-xxlcdbqi 29 24 to to TO cord-104239-xxlcdbqi 29 25 be be VB cord-104239-xxlcdbqi 29 26 evolutionarily evolutionarily RB cord-104239-xxlcdbqi 29 27 conserved conserve VBN cord-104239-xxlcdbqi 29 28 in in IN cord-104239-xxlcdbqi 29 29 yeast yeast NN cord-104239-xxlcdbqi 29 30 and and CC cord-104239-xxlcdbqi 29 31 mammalian mammalian JJ cord-104239-xxlcdbqi 29 32 cells cell NNS cord-104239-xxlcdbqi 29 33 ( ( -LRB- cord-104239-xxlcdbqi 29 34 for for IN cord-104239-xxlcdbqi 29 35 review review NN cord-104239-xxlcdbqi 29 36 see see VBP cord-104239-xxlcdbqi 29 37 Barlowe Barlowe NNP cord-104239-xxlcdbqi 29 38 , , , cord-104239-xxlcdbqi 29 39 1995 1995 CD cord-104239-xxlcdbqi 29 40 ) ) -RRB- cord-104239-xxlcdbqi 29 41 . . . cord-104239-xxlcdbqi 30 1 While while IN cord-104239-xxlcdbqi 30 2 the the DT cord-104239-xxlcdbqi 30 3 formation formation NN cord-104239-xxlcdbqi 30 4 of of IN cord-104239-xxlcdbqi 30 5 ER ER NNP cord-104239-xxlcdbqi 30 6 to to IN cord-104239-xxlcdbqi 30 7 Golgi Golgi NNP cord-104239-xxlcdbqi 30 8 carrier carrier NN cord-104239-xxlcdbqi 30 9 vesicles vesicle NNS cord-104239-xxlcdbqi 30 10 in in IN cord-104239-xxlcdbqi 30 11 secretory secretory JJ cord-104239-xxlcdbqi 30 12 tissues tissue NNS cord-104239-xxlcdbqi 30 13 is be VBZ cord-104239-xxlcdbqi 30 14 largely largely RB cord-104239-xxlcdbqi 30 15 confined confine VBN cord-104239-xxlcdbqi 30 16 to to IN cord-104239-xxlcdbqi 30 17 the the DT cord-104239-xxlcdbqi 30 18 transitional transitional JJ cord-104239-xxlcdbqi 30 19 region region NN cord-104239-xxlcdbqi 30 20 facing face VBG cord-104239-xxlcdbqi 30 21 the the DT cord-104239-xxlcdbqi 30 22 juxtanuclear juxtanuclear NNP cord-104239-xxlcdbqi 30 23 Golgi Golgi NNP cord-104239-xxlcdbqi 30 24 apparatus apparatus NN cord-104239-xxlcdbqi 30 25 ( ( -LRB- cord-104239-xxlcdbqi 30 26 Palade Palade NNP cord-104239-xxlcdbqi 30 27 , , , cord-104239-xxlcdbqi 30 28 1975 1975 CD cord-104239-xxlcdbqi 30 29 ) ) -RRB- cord-104239-xxlcdbqi 30 30 , , , cord-104239-xxlcdbqi 30 31 studies study NNS cord-104239-xxlcdbqi 30 32 in in IN cord-104239-xxlcdbqi 30 33 other other JJ cord-104239-xxlcdbqi 30 34 cell cell NN cord-104239-xxlcdbqi 30 35 lines line NNS cord-104239-xxlcdbqi 30 36 have have VBP cord-104239-xxlcdbqi 30 37 shown show VBN cord-104239-xxlcdbqi 30 38 that that DT cord-104239-xxlcdbqi 30 39 export export NN cord-104239-xxlcdbqi 30 40 from from IN cord-104239-xxlcdbqi 30 41 the the DT cord-104239-xxlcdbqi 30 42 ER ER NNP cord-104239-xxlcdbqi 30 43 can can MD cord-104239-xxlcdbqi 30 44 originate originate VB cord-104239-xxlcdbqi 30 45 from from IN cord-104239-xxlcdbqi 30 46 multiple multiple JJ cord-104239-xxlcdbqi 30 47 sites site NNS cord-104239-xxlcdbqi 30 48 that that WDT cord-104239-xxlcdbqi 30 49 appear appear VBP cord-104239-xxlcdbqi 30 50 randomly randomly RB cord-104239-xxlcdbqi 30 51 distributed distribute VBN cord-104239-xxlcdbqi 30 52 throughout throughout IN cord-104239-xxlcdbqi 30 53 the the DT cord-104239-xxlcdbqi 30 54 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 30 55 and and CC cord-104239-xxlcdbqi 30 56 , , , cord-104239-xxlcdbqi 30 57 in in IN cord-104239-xxlcdbqi 30 58 most most JJS cord-104239-xxlcdbqi 30 59 instances instance NNS cord-104239-xxlcdbqi 30 60 , , , cord-104239-xxlcdbqi 30 61 distant distant JJ cord-104239-xxlcdbqi 30 62 from from IN cord-104239-xxlcdbqi 30 63 the the DT cord-104239-xxlcdbqi 30 64 Golgi Golgi NNP cord-104239-xxlcdbqi 30 65 complex complex NN cord-104239-xxlcdbqi 30 66 . . . cord-104239-xxlcdbqi 31 1 The the DT cord-104239-xxlcdbqi 31 2 relationship relationship NN cord-104239-xxlcdbqi 31 3 of of IN cord-104239-xxlcdbqi 31 4 these these DT cord-104239-xxlcdbqi 31 5 peripheral peripheral JJ cord-104239-xxlcdbqi 31 6 sites site NNS cord-104239-xxlcdbqi 31 7 to to IN cord-104239-xxlcdbqi 31 8 the the DT cord-104239-xxlcdbqi 31 9 transitional transitional JJ cord-104239-xxlcdbqi 31 10 region region NN cord-104239-xxlcdbqi 31 11 found find VBN cord-104239-xxlcdbqi 31 12 in in IN cord-104239-xxlcdbqi 31 13 secretory secretory JJ cord-104239-xxlcdbqi 31 14 cells cell NNS cord-104239-xxlcdbqi 31 15 is be VBZ cord-104239-xxlcdbqi 31 16 unknown unknown JJ cord-104239-xxlcdbqi 31 17 , , , cord-104239-xxlcdbqi 31 18 although although IN cord-104239-xxlcdbqi 31 19 they -PRON- PRP cord-104239-xxlcdbqi 31 20 are be VBP cord-104239-xxlcdbqi 31 21 now now RB cord-104239-xxlcdbqi 31 22 recognized recognize VBN cord-104239-xxlcdbqi 31 23 to to TO cord-104239-xxlcdbqi 31 24 consist consist VB cord-104239-xxlcdbqi 31 25 of of IN cord-104239-xxlcdbqi 31 26 clusters cluster NNS cord-104239-xxlcdbqi 31 27 of of IN cord-104239-xxlcdbqi 31 28 small small JJ cord-104239-xxlcdbqi 31 29 vesicles vesicle NNS cord-104239-xxlcdbqi 31 30 and and CC cord-104239-xxlcdbqi 31 31 tubular tubular JJ cord-104239-xxlcdbqi 31 32 elements element NNS cord-104239-xxlcdbqi 31 33 ( ( -LRB- cord-104239-xxlcdbqi 31 34 Saraste Saraste NNP cord-104239-xxlcdbqi 31 35 and and CC cord-104239-xxlcdbqi 31 36 Kuismanen Kuismanen NNP cord-104239-xxlcdbqi 31 37 , , , cord-104239-xxlcdbqi 31 38 1984 1984 CD cord-104239-xxlcdbqi 31 39 ; ; : cord-104239-xxlcdbqi 31 40 Schweizer Schweizer NNP cord-104239-xxlcdbqi 31 41 et et NNP cord-104239-xxlcdbqi 31 42 al al NNP cord-104239-xxlcdbqi 31 43 . . NNP cord-104239-xxlcdbqi 31 44 , , , cord-104239-xxlcdbqi 31 45 1990 1990 CD cord-104239-xxlcdbqi 31 46 ; ; : cord-104239-xxlcdbqi 31 47 Saraste Saraste NNP cord-104239-xxlcdbqi 31 48 and and CC cord-104239-xxlcdbqi 31 49 Svensson Svensson NNP cord-104239-xxlcdbqi 31 50 , , , cord-104239-xxlcdbqi 31 51 1991 1991 CD cord-104239-xxlcdbqi 31 52 ; ; : cord-104239-xxlcdbqi 31 53 Lotti Lotti NNP cord-104239-xxlcdbqi 31 54 et et NNP cord-104239-xxlcdbqi 31 55 al al NNP cord-104239-xxlcdbqi 31 56 . . NNP cord-104239-xxlcdbqi 31 57 , , , cord-104239-xxlcdbqi 31 58 1992 1992 CD cord-104239-xxlcdbqi 31 59 ) ) -RRB- cord-104239-xxlcdbqi 32 1 re re VBG cord-104239-xxlcdbqi 32 2 - - VBN cord-104239-xxlcdbqi 32 3 ferred ferre VBN cord-104239-xxlcdbqi 32 4 to to IN cord-104239-xxlcdbqi 32 5 as as RB cord-104239-xxlcdbqi 32 6 vesicular vesicular JJ cord-104239-xxlcdbqi 32 7 tubular tubular JJ cord-104239-xxlcdbqi 32 8 clusters cluster NNS cord-104239-xxlcdbqi 32 9 ( ( -LRB- cord-104239-xxlcdbqi 32 10 VTCs VTCs NNP cord-104239-xxlcdbqi 32 11 ) ) -RRB- cord-104239-xxlcdbqi 32 12 1 1 CD cord-104239-xxlcdbqi 32 13 . . . cord-104239-xxlcdbqi 33 1 While while IN cord-104239-xxlcdbqi 33 2 VTCs vtc NNS cord-104239-xxlcdbqi 33 3 are be VBP cord-104239-xxlcdbqi 33 4 readily readily RB cord-104239-xxlcdbqi 33 5 detectable detectable JJ cord-104239-xxlcdbqi 33 6 at at IN cord-104239-xxlcdbqi 33 7 37 37 CD cord-104239-xxlcdbqi 33 8 ° ° NN cord-104239-xxlcdbqi 33 9 C c NN cord-104239-xxlcdbqi 33 10 in in IN cord-104239-xxlcdbqi 33 11 vivo vivo NN cord-104239-xxlcdbqi 33 12 ( ( -LRB- cord-104239-xxlcdbqi 33 13 Saraste Saraste NNP cord-104239-xxlcdbqi 33 14 and and CC cord-104239-xxlcdbqi 33 15 Kuismanen Kuismanen NNP cord-104239-xxlcdbqi 33 16 , , , cord-104239-xxlcdbqi 33 17 1984 1984 CD cord-104239-xxlcdbqi 33 18 ; ; : cord-104239-xxlcdbqi 33 19 Saraste Saraste NNP cord-104239-xxlcdbqi 33 20 and and CC cord-104239-xxlcdbqi 33 21 Svensson Svensson NNP cord-104239-xxlcdbqi 33 22 , , , cord-104239-xxlcdbqi 33 23 1991 1991 CD cord-104239-xxlcdbqi 33 24 ) ) -RRB- cord-104239-xxlcdbqi 33 25 and and CC cord-104239-xxlcdbqi 33 26 at at IN cord-104239-xxlcdbqi 33 27 32 32 CD cord-104239-xxlcdbqi 33 28 ° ° NN cord-104239-xxlcdbqi 33 29 C c NN cord-104239-xxlcdbqi 33 30 in in IN cord-104239-xxlcdbqi 33 31 vitro vitro FW cord-104239-xxlcdbqi 34 1 ( ( -LRB- cord-104239-xxlcdbqi 34 2 Plutner Plutner NNP cord-104239-xxlcdbqi 34 3 et et NNP cord-104239-xxlcdbqi 34 4 al al NNP cord-104239-xxlcdbqi 34 5 . . NNP cord-104239-xxlcdbqi 34 6 , , , cord-104239-xxlcdbqi 34 7 1992 1992 CD cord-104239-xxlcdbqi 35 1 ; ; : cord-104239-xxlcdbqi 35 2 Pind Pind NNP cord-104239-xxlcdbqi 35 3 et et NNP cord-104239-xxlcdbqi 35 4 al al NNP cord-104239-xxlcdbqi 35 5 . . NNP cord-104239-xxlcdbqi 35 6 , , , cord-104239-xxlcdbqi 35 7 1994a 1994a CD cord-104239-xxlcdbqi 35 8 ) ) -RRB- cord-104239-xxlcdbqi 35 9 , , , cord-104239-xxlcdbqi 35 10 visualization visualization NN cord-104239-xxlcdbqi 35 11 of of IN cord-104239-xxlcdbqi 35 12 these these DT cord-104239-xxlcdbqi 35 13 structures structure NNS cord-104239-xxlcdbqi 35 14 can can MD cord-104239-xxlcdbqi 35 15 be be VB cord-104239-xxlcdbqi 35 16 markedly markedly RB cord-104239-xxlcdbqi 35 17 enhanced enhance VBN cord-104239-xxlcdbqi 35 18 by by IN cord-104239-xxlcdbqi 35 19 incubation incubation NN cord-104239-xxlcdbqi 35 20 of of IN cord-104239-xxlcdbqi 35 21 cells cell NNS cord-104239-xxlcdbqi 35 22 at at IN cord-104239-xxlcdbqi 35 23 reduced reduce VBN cord-104239-xxlcdbqi 35 24 temperature temperature NN cord-104239-xxlcdbqi 35 25 ( ( -LRB- cord-104239-xxlcdbqi 35 26 15 15 CD cord-104239-xxlcdbqi 35 27 ° ° NN cord-104239-xxlcdbqi 35 28 -16 -16 NNP cord-104239-xxlcdbqi 35 29 ° ° NN cord-104239-xxlcdbqi 35 30 C c NN cord-104239-xxlcdbqi 35 31 ) ) -RRB- cord-104239-xxlcdbqi 35 32 ( ( -LRB- cord-104239-xxlcdbqi 35 33 Saraste Saraste NNP cord-104239-xxlcdbqi 35 34 and and CC cord-104239-xxlcdbqi 35 35 Kuismanen Kuismanen NNP cord-104239-xxlcdbqi 35 36 , , , cord-104239-xxlcdbqi 35 37 1984 1984 CD cord-104239-xxlcdbqi 35 38 ) ) -RRB- cord-104239-xxlcdbqi 35 39 , , , cord-104239-xxlcdbqi 35 40 presumably presumably RB cord-104239-xxlcdbqi 35 41 due due IN cord-104239-xxlcdbqi 35 42 to to IN cord-104239-xxlcdbqi 35 43 a a DT cord-104239-xxlcdbqi 35 44 rate rate NN cord-104239-xxlcdbqi 35 45 - - HYPH cord-104239-xxlcdbqi 35 46 limiting limit VBG cord-104239-xxlcdbqi 35 47 step step NN cord-104239-xxlcdbqi 35 48 in in IN cord-104239-xxlcdbqi 35 49 membrane membrane NN cord-104239-xxlcdbqi 35 50 flow flow NN cord-104239-xxlcdbqi 35 51 through through IN cord-104239-xxlcdbqi 35 52 these these DT cord-104239-xxlcdbqi 35 53 intermediates intermediate NNS cord-104239-xxlcdbqi 35 54 . . . cord-104239-xxlcdbqi 36 1 Elements element NNS cord-104239-xxlcdbqi 36 2 of of IN cord-104239-xxlcdbqi 36 3 VTCs vtc NNS cord-104239-xxlcdbqi 36 4 lack lack VBP cord-104239-xxlcdbqi 36 5 luminal luminal JJ cord-104239-xxlcdbqi 36 6 continuity continuity NN cord-104239-xxlcdbqi 36 7 with with IN cord-104239-xxlcdbqi 36 8 the the DT cord-104239-xxlcdbqi 36 9 ER ER NNP cord-104239-xxlcdbqi 36 10 ( ( -LRB- cord-104239-xxlcdbqi 36 11 Saraste Saraste NNP cord-104239-xxlcdbqi 36 12 and and CC cord-104239-xxlcdbqi 36 13 Svensson Svensson NNP cord-104239-xxlcdbqi 36 14 , , , cord-104239-xxlcdbqi 36 15 1991 1991 CD cord-104239-xxlcdbqi 36 16 ; ; : cord-104239-xxlcdbqi 36 17 Balch Balch NNP cord-104239-xxlcdbqi 36 18 et et NNP cord-104239-xxlcdbqi 36 19 al al NNP cord-104239-xxlcdbqi 36 20 . . NNP cord-104239-xxlcdbqi 36 21 , , , cord-104239-xxlcdbqi 36 22 1994 1994 CD cord-104239-xxlcdbqi 36 23 ; ; . cord-104239-xxlcdbqi 37 1 Connolly Connolly NNP cord-104239-xxlcdbqi 37 2 et et NNP cord-104239-xxlcdbqi 37 3 al al NNP cord-104239-xxlcdbqi 37 4 . . NNP cord-104239-xxlcdbqi 37 5 , , , cord-104239-xxlcdbqi 37 6 1994 1994 CD cord-104239-xxlcdbqi 37 7 ) ) -RRB- cord-104239-xxlcdbqi 37 8 , , , cord-104239-xxlcdbqi 37 9 although although IN cord-104239-xxlcdbqi 37 10 tubular tubular JJ cord-104239-xxlcdbqi 37 11 extensions extension NNS cord-104239-xxlcdbqi 37 12 of of IN cord-104239-xxlcdbqi 37 13 ER ER NNP cord-104239-xxlcdbqi 37 14 into into IN cord-104239-xxlcdbqi 37 15 these these DT cord-104239-xxlcdbqi 37 16 structures structure NNS cord-104239-xxlcdbqi 37 17 have have VBP cord-104239-xxlcdbqi 37 18 been be VBN cord-104239-xxlcdbqi 37 19 observed observe VBN cord-104239-xxlcdbqi 38 1 ( ( -LRB- cord-104239-xxlcdbqi 38 2 Stinchcombe Stinchcombe NNP cord-104239-xxlcdbqi 38 3 et et NNP cord-104239-xxlcdbqi 38 4 al al NNP cord-104239-xxlcdbqi 38 5 . . NNP cord-104239-xxlcdbqi 38 6 , , , cord-104239-xxlcdbqi 38 7 1995 1995 CD cord-104239-xxlcdbqi 38 8 ) ) -RRB- cord-104239-xxlcdbqi 38 9 , , , cord-104239-xxlcdbqi 38 10 particularly particularly RB cord-104239-xxlcdbqi 38 11 in in IN cord-104239-xxlcdbqi 38 12 cells cell NNS cord-104239-xxlcdbqi 38 13 infected infect VBN cord-104239-xxlcdbqi 38 14 with with IN cord-104239-xxlcdbqi 38 15 certain certain JJ cord-104239-xxlcdbqi 38 16 viruses virus NNS cord-104239-xxlcdbqi 38 17 ( ( -LRB- cord-104239-xxlcdbqi 38 18 Tooze Tooze NNP cord-104239-xxlcdbqi 38 19 et et NNP cord-104239-xxlcdbqi 38 20 al al NNP cord-104239-xxlcdbqi 38 21 . . NNP cord-104239-xxlcdbqi 38 22 , , , cord-104239-xxlcdbqi 38 23 1984 1984 CD cord-104239-xxlcdbqi 38 24 ; ; : cord-104239-xxlcdbqi 38 25 Krijnse Krijnse NNP cord-104239-xxlcdbqi 38 26 - - HYPH cord-104239-xxlcdbqi 38 27 Locker Locker NNP cord-104239-xxlcdbqi 38 28 et et FW cord-104239-xxlcdbqi 38 29 al al NNP cord-104239-xxlcdbqi 38 30 . . NNP cord-104239-xxlcdbqi 38 31 , , , cord-104239-xxlcdbqi 38 32 1994 1994 CD cord-104239-xxlcdbqi 38 33 ) ) -RRB- cord-104239-xxlcdbqi 38 34 , , , cord-104239-xxlcdbqi 38 35 reinforcing reinforce VBG cord-104239-xxlcdbqi 38 36 their -PRON- PRP$ cord-104239-xxlcdbqi 38 37 close close JJ cord-104239-xxlcdbqi 38 38 relationship relationship NN cord-104239-xxlcdbqi 38 39 to to IN cord-104239-xxlcdbqi 38 40 ER ER NNP cord-104239-xxlcdbqi 38 41 export export NN cord-104239-xxlcdbqi 38 42 . . . cord-104239-xxlcdbqi 39 1 VTCs VTCs NNP cord-104239-xxlcdbqi 39 2 are be VBP cord-104239-xxlcdbqi 39 3 dynamic dynamic JJ cord-104239-xxlcdbqi 39 4 structures structure NNS cord-104239-xxlcdbqi 39 5 with with IN cord-104239-xxlcdbqi 39 6 varied varied JJ cord-104239-xxlcdbqi 39 7 morphology morphology NN cord-104239-xxlcdbqi 39 8 in in IN cord-104239-xxlcdbqi 39 9 different different JJ cord-104239-xxlcdbqi 39 10 cell cell NN cord-104239-xxlcdbqi 39 11 types type NNS cord-104239-xxlcdbqi 39 12 . . . cord-104239-xxlcdbqi 40 1 In in IN cord-104239-xxlcdbqi 40 2 the the DT cord-104239-xxlcdbqi 40 3 past past NN cord-104239-xxlcdbqi 40 4 , , , cord-104239-xxlcdbqi 40 5 VTCs vtc NNS cord-104239-xxlcdbqi 40 6 have have VBP cord-104239-xxlcdbqi 40 7 been be VBN cord-104239-xxlcdbqi 40 8 suggested suggest VBN cord-104239-xxlcdbqi 40 9 to to TO cord-104239-xxlcdbqi 40 10 be be VB cord-104239-xxlcdbqi 40 11 the the DT cord-104239-xxlcdbqi 40 12 site site NN cord-104239-xxlcdbqi 40 13 of of IN cord-104239-xxlcdbqi 40 14 O o NN cord-104239-xxlcdbqi 40 15 - - HYPH cord-104239-xxlcdbqi 40 16 glycosylation glycosylation NN cord-104239-xxlcdbqi 41 1 ( ( -LRB- cord-104239-xxlcdbqi 41 2 Tooze Tooze NNP cord-104239-xxlcdbqi 41 3 et et NNP cord-104239-xxlcdbqi 41 4 al al NNP cord-104239-xxlcdbqi 41 5 . . NNP cord-104239-xxlcdbqi 41 6 , , , cord-104239-xxlcdbqi 41 7 1988 1988 CD cord-104239-xxlcdbqi 41 8 ) ) -RRB- cord-104239-xxlcdbqi 41 9 , , , cord-104239-xxlcdbqi 41 10 acylation acylation NN cord-104239-xxlcdbqi 41 11 ( ( -LRB- cord-104239-xxlcdbqi 41 12 Rizzolo Rizzolo NNP cord-104239-xxlcdbqi 41 13 et et FW cord-104239-xxlcdbqi 41 14 al al NNP cord-104239-xxlcdbqi 41 15 . . NNP cord-104239-xxlcdbqi 41 16 , , , cord-104239-xxlcdbqi 41 17 1985 1985 CD cord-104239-xxlcdbqi 41 18 ) ) -RRB- cord-104239-xxlcdbqi 41 19 , , , cord-104239-xxlcdbqi 41 20 and and CC cord-104239-xxlcdbqi 41 21 generation generation NN cord-104239-xxlcdbqi 41 22 of of IN cord-104239-xxlcdbqi 41 23 the the DT cord-104239-xxlcdbqi 41 24 mannose-6-phosphate mannose-6-phosphate NNP cord-104239-xxlcdbqi 41 25 signal signal NN cord-104239-xxlcdbqi 41 26 for for IN cord-104239-xxlcdbqi 41 27 lysosomal lysosomal JJ cord-104239-xxlcdbqi 41 28 protein protein NN cord-104239-xxlcdbqi 41 29 targeting target VBG cord-104239-xxlcdbqi 41 30 ( ( -LRB- cord-104239-xxlcdbqi 41 31 Pelham Pelham NNP cord-104239-xxlcdbqi 41 32 , , , cord-104239-xxlcdbqi 41 33 1988 1988 CD cord-104239-xxlcdbqi 41 34 ) ) -RRB- cord-104239-xxlcdbqi 41 35 . . . cord-104239-xxlcdbqi 42 1 Several several JJ cord-104239-xxlcdbqi 42 2 endogenous endogenous JJ cord-104239-xxlcdbqi 42 3 proteins protein NNS cord-104239-xxlcdbqi 42 4 serve serve VBP cord-104239-xxlcdbqi 42 5 as as IN cord-104239-xxlcdbqi 42 6 useful useful JJ cord-104239-xxlcdbqi 42 7 markers marker NNS cord-104239-xxlcdbqi 42 8 for for IN cord-104239-xxlcdbqi 42 9 VTCs vtc NNS cord-104239-xxlcdbqi 42 10 . . . cord-104239-xxlcdbqi 43 1 These these DT cord-104239-xxlcdbqi 43 2 include include VBP cord-104239-xxlcdbqi 43 3 the the DT cord-104239-xxlcdbqi 43 4 small small JJ cord-104239-xxlcdbqi 43 5 GTPase GTPase NNP cord-104239-xxlcdbqi 43 6 Rab2 Rab2 NNP cord-104239-xxlcdbqi 43 7 ( ( -LRB- cord-104239-xxlcdbqi 43 8 Chavrier Chavrier NNP cord-104239-xxlcdbqi 43 9 et et NNP cord-104239-xxlcdbqi 43 10 al al NNP cord-104239-xxlcdbqi 43 11 . . NNP cord-104239-xxlcdbqi 43 12 , , , cord-104239-xxlcdbqi 43 13 1990 1990 CD cord-104239-xxlcdbqi 43 14 ) ) -RRB- cord-104239-xxlcdbqi 43 15 , , , cord-104239-xxlcdbqi 43 16 the the DT cord-104239-xxlcdbqi 43 17 transmembrane transmembrane NNP cord-104239-xxlcdbqi 43 18 protein protein NN cord-104239-xxlcdbqi 43 19 p58 p58 NN cord-104239-xxlcdbqi 43 20 in in IN cord-104239-xxlcdbqi 43 21 rat rat NN cord-104239-xxlcdbqi 43 22 cells cell NNS cord-104239-xxlcdbqi 43 23 or or CC cord-104239-xxlcdbqi 43 24 its -PRON- PRP$ cord-104239-xxlcdbqi 43 25 human human JJ cord-104239-xxlcdbqi 43 26 homologue homologue NN cord-104239-xxlcdbqi 43 27 p53 p53 NN cord-104239-xxlcdbqi 43 28 ( ( -LRB- cord-104239-xxlcdbqi 43 29 Schweizer Schweizer NNP cord-104239-xxlcdbqi 43 30 et et NNP cord-104239-xxlcdbqi 43 31 al al NNP cord-104239-xxlcdbqi 43 32 . . NNP cord-104239-xxlcdbqi 43 33 , , , cord-104239-xxlcdbqi 43 34 1988 1988 CD cord-104239-xxlcdbqi 43 35 ; ; : cord-104239-xxlcdbqi 43 36 Saraste Saraste NNP cord-104239-xxlcdbqi 43 37 and and CC cord-104239-xxlcdbqi 43 38 Svensson Svensson NNP cord-104239-xxlcdbqi 43 39 , , , cord-104239-xxlcdbqi 43 40 1991 1991 CD cord-104239-xxlcdbqi 43 41 ) ) -RRB- cord-104239-xxlcdbqi 43 42 , , , cord-104239-xxlcdbqi 43 43 which which WDT cord-104239-xxlcdbqi 43 44 actively actively RB cord-104239-xxlcdbqi 43 45 cycle cycle VBP cord-104239-xxlcdbqi 43 46 between between IN cord-104239-xxlcdbqi 43 47 the the DT cord-104239-xxlcdbqi 43 48 ER ER NNP cord-104239-xxlcdbqi 43 49 and and CC cord-104239-xxlcdbqi 43 50 VTCs vtc NNS cord-104239-xxlcdbqi 43 51 , , , cord-104239-xxlcdbqi 43 52 and and CC cord-104239-xxlcdbqi 43 53 the the DT cord-104239-xxlcdbqi 43 54 COPI COPI NNP cord-104239-xxlcdbqi 43 55 subunit subunit NN cord-104239-xxlcdbqi 43 56 13-COP 13-cop CD cord-104239-xxlcdbqi 44 1 ( ( -LRB- cord-104239-xxlcdbqi 44 2 Oprins Oprins NNP cord-104239-xxlcdbqi 44 3 et et NNP cord-104239-xxlcdbqi 44 4 al al NNP cord-104239-xxlcdbqi 44 5 . . . cord-104239-xxlcdbqi 45 1 , , , cord-104239-xxlcdbqi 45 2 1993 1993 CD cord-104239-xxlcdbqi 45 3 ; ; : cord-104239-xxlcdbqi 45 4 Pepperkok Pepperkok NNP cord-104239-xxlcdbqi 45 5 et et NNP cord-104239-xxlcdbqi 45 6 al al NNP cord-104239-xxlcdbqi 45 7 . . . cord-104239-xxlcdbqi 46 1 , , , cord-104239-xxlcdbqi 46 2 1993 1993 CD cord-104239-xxlcdbqi 46 3 ; ; : cord-104239-xxlcdbqi 46 4 Pind Pind NNP cord-104239-xxlcdbqi 46 5 et et NNP cord-104239-xxlcdbqi 46 6 al al NNP cord-104239-xxlcdbqi 46 7 . . . cord-104239-xxlcdbqi 47 1 , , , cord-104239-xxlcdbqi 47 2 1994a 1994a CD cord-104239-xxlcdbqi 47 3 ; ; : cord-104239-xxlcdbqi 47 4 Aridor Aridor NNP cord-104239-xxlcdbqi 47 5 et et NNP cord-104239-xxlcdbqi 47 6 al al NNP cord-104239-xxlcdbqi 47 7 . . NNP cord-104239-xxlcdbqi 47 8 , , , cord-104239-xxlcdbqi 47 9 1995 1995 CD cord-104239-xxlcdbqi 47 10 ) ) -RRB- cord-104239-xxlcdbqi 47 11 . . . cord-104239-xxlcdbqi 48 1 VTCs vtc NNS cord-104239-xxlcdbqi 48 2 play play VBP cord-104239-xxlcdbqi 48 3 a a DT cord-104239-xxlcdbqi 48 4 pivotal pivotal JJ cord-104239-xxlcdbqi 48 5 role role NN cord-104239-xxlcdbqi 48 6 in in IN cord-104239-xxlcdbqi 48 7 the the DT cord-104239-xxlcdbqi 48 8 segregation segregation NN cord-104239-xxlcdbqi 48 9 of of IN cord-104239-xxlcdbqi 48 10 anterograde anterograde JJ cord-104239-xxlcdbqi 48 11 and and CC cord-104239-xxlcdbqi 48 12 retrograde retrograde JJ cord-104239-xxlcdbqi 48 13 transported transport VBN cord-104239-xxlcdbqi 48 14 proteins protein NNS cord-104239-xxlcdbqi 49 1 ( ( -LRB- cord-104239-xxlcdbqi 49 2 Aridor Aridor NNP cord-104239-xxlcdbqi 49 3 et et NNP cord-104239-xxlcdbqi 49 4 al al NNP cord-104239-xxlcdbqi 49 5 . . . cord-104239-xxlcdbqi 50 1 , , , cord-104239-xxlcdbqi 50 2 1995 1995 CD cord-104239-xxlcdbqi 50 3 ; ; : cord-104239-xxlcdbqi 50 4 Tang Tang NNP cord-104239-xxlcdbqi 50 5 et et NNP cord-104239-xxlcdbqi 50 6 al al NNP cord-104239-xxlcdbqi 50 7 . . NNP cord-104239-xxlcdbqi 50 8 , , , cord-104239-xxlcdbqi 50 9 1995 1995 CD cord-104239-xxlcdbqi 50 10 ) ) -RRB- cord-104239-xxlcdbqi 50 11 . . . cord-104239-xxlcdbqi 51 1 Segregation segregation NN cord-104239-xxlcdbqi 51 2 is be VBZ cord-104239-xxlcdbqi 51 3 believed believe VBN cord-104239-xxlcdbqi 51 4 to to TO cord-104239-xxlcdbqi 51 5 involve involve VB cord-104239-xxlcdbqi 51 6 the the DT cord-104239-xxlcdbqi 51 7 COPI COPI NNP cord-104239-xxlcdbqi 51 8 coat coat NN cord-104239-xxlcdbqi 51 9 complex complex NN cord-104239-xxlcdbqi 51 10 whose whose WP$ cord-104239-xxlcdbqi 51 11 assembly assembly NN cord-104239-xxlcdbqi 51 12 is be VBZ cord-104239-xxlcdbqi 51 13 driven drive VBN cord-104239-xxlcdbqi 51 14 through through IN cord-104239-xxlcdbqi 51 15 activation activation NN cord-104239-xxlcdbqi 51 16 of of IN cord-104239-xxlcdbqi 51 17 the the DT cord-104239-xxlcdbqi 51 18 ARF1 ARF1 NNP cord-104239-xxlcdbqi 51 19 GTPase GTPase NNP cord-104239-xxlcdbqi 52 1 ( ( -LRB- cord-104239-xxlcdbqi 52 2 Aridor Aridor NNP cord-104239-xxlcdbqi 52 3 et et NNP cord-104239-xxlcdbqi 52 4 al al NNP cord-104239-xxlcdbqi 52 5 . . . cord-104239-xxlcdbqi 53 1 , , , cord-104239-xxlcdbqi 53 2 1995 1995 CD cord-104239-xxlcdbqi 53 3 ; ; : cord-104239-xxlcdbqi 53 4 Letourneur Letourneur NNP cord-104239-xxlcdbqi 53 5 et et NNP cord-104239-xxlcdbqi 53 6 al al NNP cord-104239-xxlcdbqi 53 7 . . NNP cord-104239-xxlcdbqi 53 8 , , , cord-104239-xxlcdbqi 53 9 1995 1995 CD cord-104239-xxlcdbqi 53 10 ) ) -RRB- cord-104239-xxlcdbqi 53 11 . . . cord-104239-xxlcdbqi 54 1 While while IN cord-104239-xxlcdbqi 54 2 VTC VTC NNP cord-104239-xxlcdbqi 54 3 composition composition NN cord-104239-xxlcdbqi 54 4 and and CC cord-104239-xxlcdbqi 54 5 function function NN cord-104239-xxlcdbqi 54 6 have have VBP cord-104239-xxlcdbqi 54 7 been be VBN cord-104239-xxlcdbqi 54 8 described describe VBN cord-104239-xxlcdbqi 54 9 qualitatively qualitatively RB cord-104239-xxlcdbqi 54 10 through through IN cord-104239-xxlcdbqi 54 11 use use NN cord-104239-xxlcdbqi 54 12 of of IN cord-104239-xxlcdbqi 54 13 immunofluorescence immunofluorescence NN cord-104239-xxlcdbqi 54 14 ( ( -LRB- cord-104239-xxlcdbqi 54 15 Lotti Lotti NNP cord-104239-xxlcdbqi 54 16 et et NNP cord-104239-xxlcdbqi 54 17 al al NNP cord-104239-xxlcdbqi 54 18 . . NNP cord-104239-xxlcdbqi 54 19 , , , cord-104239-xxlcdbqi 54 20 1992 1992 CD cord-104239-xxlcdbqi 54 21 ; ; . cord-104239-xxlcdbqi 55 1 Aridor Aridor NNP cord-104239-xxlcdbqi 55 2 et et NNP cord-104239-xxlcdbqi 55 3 al al NNP cord-104239-xxlcdbqi 55 4 . . . cord-104239-xxlcdbqi 56 1 , , , cord-104239-xxlcdbqi 56 2 1995 1995 CD cord-104239-xxlcdbqi 56 3 ; ; : cord-104239-xxlcdbqi 56 4 Lippincott Lippincott NNP cord-104239-xxlcdbqi 56 5 - - HYPH cord-104239-xxlcdbqi 56 6 Schwartz Schwartz NNP cord-104239-xxlcdbqi 56 7 et et NNP cord-104239-xxlcdbqi 56 8 al al NNP cord-104239-xxlcdbqi 56 9 . . NNP cord-104239-xxlcdbqi 56 10 , , , cord-104239-xxlcdbqi 56 11 1995 1995 CD cord-104239-xxlcdbqi 56 12 ) ) -RRB- cord-104239-xxlcdbqi 56 13 and and CC cord-104239-xxlcdbqi 56 14 immunoperoxidase immunoperoxidase NNP cord-104239-xxlcdbqi 57 1 ( ( -LRB- cord-104239-xxlcdbqi 57 2 Connolly Connolly NNP cord-104239-xxlcdbqi 57 3 et et NNP cord-104239-xxlcdbqi 57 4 al al NNP cord-104239-xxlcdbqi 57 5 . . NNP cord-104239-xxlcdbqi 57 6 , , , cord-104239-xxlcdbqi 57 7 1994 1994 CD cord-104239-xxlcdbqi 58 1 ; ; : cord-104239-xxlcdbqi 58 2 Stinchcombe Stinchcombe NNP cord-104239-xxlcdbqi 58 3 et et NNP cord-104239-xxlcdbqi 58 4 al al NNP cord-104239-xxlcdbqi 58 5 . . NNP cord-104239-xxlcdbqi 58 6 , , , cord-104239-xxlcdbqi 58 7 1995 1995 CD cord-104239-xxlcdbqi 58 8 ) ) -RRB- cord-104239-xxlcdbqi 58 9 approaches approach VBZ cord-104239-xxlcdbqi 58 10 , , , cord-104239-xxlcdbqi 58 11 the the DT cord-104239-xxlcdbqi 58 12 topology topology NN cord-104239-xxlcdbqi 58 13 of of IN cord-104239-xxlcdbqi 58 14 ER ER NNP cord-104239-xxlcdbqi 58 15 budding budding NN cord-104239-xxlcdbqi 58 16 sites site NNS cord-104239-xxlcdbqi 58 17 and and CC cord-104239-xxlcdbqi 58 18 , , , cord-104239-xxlcdbqi 58 19 in in IN cord-104239-xxlcdbqi 58 20 particular particular JJ cord-104239-xxlcdbqi 58 21 , , , cord-104239-xxlcdbqi 58 22 their -PRON- PRP$ cord-104239-xxlcdbqi 58 23 relationship relationship NN cord-104239-xxlcdbqi 58 24 to to IN cord-104239-xxlcdbqi 58 25 VTCs vtc NNS cord-104239-xxlcdbqi 58 26 have have VBP cord-104239-xxlcdbqi 58 27 not not RB cord-104239-xxlcdbqi 58 28 been be VBN cord-104239-xxlcdbqi 58 29 studied study VBN cord-104239-xxlcdbqi 58 30 quantitatively quantitatively RB cord-104239-xxlcdbqi 58 31 at at IN cord-104239-xxlcdbqi 58 32 high high JJ cord-104239-xxlcdbqi 58 33 resolution resolution NN cord-104239-xxlcdbqi 58 34 . . . cord-104239-xxlcdbqi 59 1 In in IN cord-104239-xxlcdbqi 59 2 the the DT cord-104239-xxlcdbqi 59 3 present present JJ cord-104239-xxlcdbqi 59 4 paper paper NN cord-104239-xxlcdbqi 59 5 , , , cord-104239-xxlcdbqi 59 6 we -PRON- PRP cord-104239-xxlcdbqi 59 7 use use VBP cord-104239-xxlcdbqi 59 8 both both DT cord-104239-xxlcdbqi 59 9 transmission transmission NN cord-104239-xxlcdbqi 59 10 TEM tem NN cord-104239-xxlcdbqi 59 11 and and CC cord-104239-xxlcdbqi 59 12 immunoelectron immunoelectron NN cord-104239-xxlcdbqi 59 13 microscopy microscopy NN cord-104239-xxlcdbqi 59 14 in in IN cord-104239-xxlcdbqi 59 15 conjunction conjunction NN cord-104239-xxlcdbqi 59 16 with with IN cord-104239-xxlcdbqi 59 17 quick quick JJ cord-104239-xxlcdbqi 59 18 - - HYPH cord-104239-xxlcdbqi 59 19 freeze freeze JJ cord-104239-xxlcdbqi 59 20 , , , cord-104239-xxlcdbqi 59 21 deep deep JJ cord-104239-xxlcdbqi 59 22 - - HYPH cord-104239-xxlcdbqi 59 23 etch etch NN cord-104239-xxlcdbqi 59 24 methods method NNS cord-104239-xxlcdbqi 59 25 to to TO cord-104239-xxlcdbqi 59 26 reconstruct reconstruct VB cord-104239-xxlcdbqi 59 27 these these DT cord-104239-xxlcdbqi 59 28 relationships relationship NNS cord-104239-xxlcdbqi 59 29 morphometrically morphometrically RB cord-104239-xxlcdbqi 59 30 and and CC cord-104239-xxlcdbqi 59 31 to to TO cord-104239-xxlcdbqi 59 32 confirm confirm VB cord-104239-xxlcdbqi 59 33 that that IN cord-104239-xxlcdbqi 59 34 cargo cargo NN cord-104239-xxlcdbqi 59 35 is be VBZ cord-104239-xxlcdbqi 59 36 concentrated concentrate VBN cord-104239-xxlcdbqi 59 37 during during IN cord-104239-xxlcdbqi 59 38 vesicle vesicle NN cord-104239-xxlcdbqi 59 39 budding bud VBG cord-104239-xxlcdbqi 59 40 . . . cord-104239-xxlcdbqi 60 1 We -PRON- PRP cord-104239-xxlcdbqi 60 2 find find VBP cord-104239-xxlcdbqi 60 3 that that IN cord-104239-xxlcdbqi 60 4 almost almost RB cord-104239-xxlcdbqi 60 5 all all DT cord-104239-xxlcdbqi 60 6 ER ER NNP cord-104239-xxlcdbqi 60 7 buds bud NNS cord-104239-xxlcdbqi 60 8 detected detect VBN cord-104239-xxlcdbqi 60 9 in in IN cord-104239-xxlcdbqi 60 10 the the DT cord-104239-xxlcdbqi 60 11 cell cell NN cord-104239-xxlcdbqi 60 12 were be VBD cord-104239-xxlcdbqi 60 13 concentrated concentrate VBN cord-104239-xxlcdbqi 60 14 in in IN cord-104239-xxlcdbqi 60 15 regional regional JJ cord-104239-xxlcdbqi 60 16 loci locus NNS cord-104239-xxlcdbqi 60 17 facing face VBG cord-104239-xxlcdbqi 60 18 VTCs vtc NNS cord-104239-xxlcdbqi 60 19 , , , cord-104239-xxlcdbqi 60 20 which which WDT cord-104239-xxlcdbqi 60 21 had have VBD cord-104239-xxlcdbqi 60 22 a a DT cord-104239-xxlcdbqi 60 23 characteristic characteristic JJ cord-104239-xxlcdbqi 60 24 diameter diameter NN cord-104239-xxlcdbqi 60 25 and and CC cord-104239-xxlcdbqi 60 26 vesicular vesicular JJ cord-104239-xxlcdbqi 60 27 - - HYPH cord-104239-xxlcdbqi 60 28 tubular tubular JJ cord-104239-xxlcdbqi 60 29 composition composition NN cord-104239-xxlcdbqi 60 30 . . . cord-104239-xxlcdbqi 61 1 As as IN cord-104239-xxlcdbqi 61 2 such such JJ cord-104239-xxlcdbqi 61 3 , , , cord-104239-xxlcdbqi 61 4 the the DT cord-104239-xxlcdbqi 61 5 topological topological JJ cord-104239-xxlcdbqi 61 6 organization organization NN cord-104239-xxlcdbqi 61 7 of of IN cord-104239-xxlcdbqi 61 8 buds bud NNS cord-104239-xxlcdbqi 61 9 and and CC cord-104239-xxlcdbqi 61 10 VTCs vtc NNS cord-104239-xxlcdbqi 61 11 defines define VBZ cord-104239-xxlcdbqi 61 12 a a DT cord-104239-xxlcdbqi 61 13 morphological morphological JJ cord-104239-xxlcdbqi 61 14 unit unit NN cord-104239-xxlcdbqi 61 15 of of IN cord-104239-xxlcdbqi 61 16 function function NN cord-104239-xxlcdbqi 61 17 , , , cord-104239-xxlcdbqi 61 18 which which WDT cord-104239-xxlcdbqi 61 19 we -PRON- PRP cord-104239-xxlcdbqi 61 20 refer refer VBP cord-104239-xxlcdbqi 61 21 to to IN cord-104239-xxlcdbqi 61 22 as as IN cord-104239-xxlcdbqi 61 23 export export NN cord-104239-xxlcdbqi 61 24 complexes complex NNS cord-104239-xxlcdbqi 61 25 . . . cord-104239-xxlcdbqi 62 1 We -PRON- PRP cord-104239-xxlcdbqi 62 2 conclude conclude VBP cord-104239-xxlcdbqi 62 3 that that IN cord-104239-xxlcdbqi 62 4 budding bud VBG cord-104239-xxlcdbqi 62 5 from from IN cord-104239-xxlcdbqi 62 6 the the DT cord-104239-xxlcdbqi 62 7 ER ER NNP cord-104239-xxlcdbqi 62 8 is be VBZ cord-104239-xxlcdbqi 62 9 not not RB cord-104239-xxlcdbqi 62 10 evenly evenly RB cord-104239-xxlcdbqi 62 11 distributed distribute VBN cord-104239-xxlcdbqi 62 12 along along IN cord-104239-xxlcdbqi 62 13 the the DT cord-104239-xxlcdbqi 62 14 membrane membrane NN cord-104239-xxlcdbqi 62 15 , , , cord-104239-xxlcdbqi 62 16 but but CC cord-104239-xxlcdbqi 62 17 is be VBZ cord-104239-xxlcdbqi 62 18 confined confine VBN cord-104239-xxlcdbqi 62 19 to to IN cord-104239-xxlcdbqi 62 20 specific specific JJ cord-104239-xxlcdbqi 62 21 export export NN cord-104239-xxlcdbqi 62 22 sites site NNS cord-104239-xxlcdbqi 62 23 . . . cord-104239-xxlcdbqi 63 1 These these DT cord-104239-xxlcdbqi 63 2 are be VBP cord-104239-xxlcdbqi 63 3 highly highly RB cord-104239-xxlcdbqi 63 4 enriched enrich VBN cord-104239-xxlcdbqi 63 5 in in IN cord-104239-xxlcdbqi 63 6 COPII COPII NNP cord-104239-xxlcdbqi 63 7 and and CC cord-104239-xxlcdbqi 63 8 COPI COPI NNP cord-104239-xxlcdbqi 63 9 transport transport NN cord-104239-xxlcdbqi 63 10 components component NNS cord-104239-xxlcdbqi 63 11 and and CC cord-104239-xxlcdbqi 63 12 are be VBP cord-104239-xxlcdbqi 63 13 likely likely JJ cord-104239-xxlcdbqi 63 14 to to TO cord-104239-xxlcdbqi 63 15 promote promote VB cord-104239-xxlcdbqi 63 16 the the DT cord-104239-xxlcdbqi 63 17 coupling coupling NN cord-104239-xxlcdbqi 63 18 of of IN cord-104239-xxlcdbqi 63 19 anterograde anterograde JJ cord-104239-xxlcdbqi 63 20 and and CC cord-104239-xxlcdbqi 63 21 retrograde retrograde JJ cord-104239-xxlcdbqi 63 22 transport transport NN cord-104239-xxlcdbqi 63 23 . . . cord-104239-xxlcdbqi 64 1 A a DT cord-104239-xxlcdbqi 64 2 polyclonal polyclonal JJ cord-104239-xxlcdbqi 64 3 antibody antibody NN cord-104239-xxlcdbqi 64 4 specific specific JJ cord-104239-xxlcdbqi 64 5 for for IN cord-104239-xxlcdbqi 64 6 yeast yeast NN cord-104239-xxlcdbqi 64 7 Sec23p Sec23p NNP cord-104239-xxlcdbqi 64 8 that that WDT cord-104239-xxlcdbqi 64 9 cross cross NN cord-104239-xxlcdbqi 64 10 - - VBZ cord-104239-xxlcdbqi 64 11 reacts react VBZ cord-104239-xxlcdbqi 64 12 with with IN cord-104239-xxlcdbqi 64 13 a a DT cord-104239-xxlcdbqi 64 14 mammalian mammalian JJ cord-104239-xxlcdbqi 64 15 homologue homologue NN cord-104239-xxlcdbqi 64 16 ( ( -LRB- cord-104239-xxlcdbqi 64 17 Orci Orci NNP cord-104239-xxlcdbqi 64 18 et et NNP cord-104239-xxlcdbqi 64 19 al al NNP cord-104239-xxlcdbqi 64 20 . . NNP cord-104239-xxlcdbqi 64 21 , , , cord-104239-xxlcdbqi 64 22 1991 1991 CD cord-104239-xxlcdbqi 64 23 ) ) -RRB- cord-104239-xxlcdbqi 64 24 was be VBD cord-104239-xxlcdbqi 64 25 obtained obtain VBN cord-104239-xxlcdbqi 64 26 from from IN cord-104239-xxlcdbqi 64 27 R. R. NNP cord-104239-xxlcdbqi 64 28 Schekman Schekman NNP cord-104239-xxlcdbqi 64 29 ( ( -LRB- cord-104239-xxlcdbqi 64 30 University University NNP cord-104239-xxlcdbqi 64 31 of of IN cord-104239-xxlcdbqi 64 32 California California NNP cord-104239-xxlcdbqi 64 33 , , , cord-104239-xxlcdbqi 64 34 Berkeley Berkeley NNP cord-104239-xxlcdbqi 64 35 ) ) -RRB- cord-104239-xxlcdbqi 64 36 . . . cord-104239-xxlcdbqi 65 1 Affinity affinity NN cord-104239-xxlcdbqi 65 2 - - HYPH cord-104239-xxlcdbqi 65 3 purified purify VBN cord-104239-xxlcdbqi 65 4 antibodies antibody NNS cord-104239-xxlcdbqi 65 5 specific specific JJ cord-104239-xxlcdbqi 65 6 for for IN cord-104239-xxlcdbqi 65 7 yeast yeast NN cord-104239-xxlcdbqi 65 8 Secl3p Secl3p NNP cord-104239-xxlcdbqi 65 9 that that DT cord-104239-xxlcdbqi 65 10 cross cross NN cord-104239-xxlcdbqi 65 11 - - JJ cord-104239-xxlcdbqi 65 12 reacts react VBZ cord-104239-xxlcdbqi 65 13 with with IN cord-104239-xxlcdbqi 65 14 a a DT cord-104239-xxlcdbqi 65 15 mammalian mammalian JJ cord-104239-xxlcdbqi 65 16 homologue homologue NN cord-104239-xxlcdbqi 65 17 ( ( -LRB- cord-104239-xxlcdbqi 65 18 Shaywitz Shaywitz NNP cord-104239-xxlcdbqi 65 19 et et FW cord-104239-xxlcdbqi 65 20 al al NNP cord-104239-xxlcdbqi 65 21 . . NNP cord-104239-xxlcdbqi 65 22 , , , cord-104239-xxlcdbqi 65 23 1995 1995 CD cord-104239-xxlcdbqi 65 24 ) ) -RRB- cord-104239-xxlcdbqi 65 25 were be VBD cord-104239-xxlcdbqi 65 26 a a DT cord-104239-xxlcdbqi 65 27 generous generous JJ cord-104239-xxlcdbqi 65 28 gift gift NN cord-104239-xxlcdbqi 65 29 of of IN cord-104239-xxlcdbqi 65 30 C. C. NNP cord-104239-xxlcdbqi 65 31 Kaiser Kaiser NNP cord-104239-xxlcdbqi 65 32 ( ( -LRB- cord-104239-xxlcdbqi 65 33 Massachusetts Massachusetts NNP cord-104239-xxlcdbqi 65 34 Institute Institute NNP cord-104239-xxlcdbqi 65 35 of of IN cord-104239-xxlcdbqi 65 36 Technology Technology NNP cord-104239-xxlcdbqi 65 37 , , , cord-104239-xxlcdbqi 65 38 Cambridge Cambridge NNP cord-104239-xxlcdbqi 65 39 , , , cord-104239-xxlcdbqi 65 40 MA MA NNP cord-104239-xxlcdbqi 65 41 ) ) -RRB- cord-104239-xxlcdbqi 65 42 . . . cord-104239-xxlcdbqi 66 1 A a DT cord-104239-xxlcdbqi 66 2 hybridoma hybridoma NN cord-104239-xxlcdbqi 66 3 cell cell NN cord-104239-xxlcdbqi 66 4 line line NN cord-104239-xxlcdbqi 66 5 expressing express VBG cord-104239-xxlcdbqi 66 6 an an DT cord-104239-xxlcdbqi 66 7 mAb mab NN cord-104239-xxlcdbqi 66 8 specific specific NN cord-104239-xxlcdbqi 66 9 for for IN cord-104239-xxlcdbqi 66 10 the the DT cord-104239-xxlcdbqi 66 11 carboxyl carboxyl NN cord-104239-xxlcdbqi 66 12 terminus terminus NN cord-104239-xxlcdbqi 66 13 of of IN cord-104239-xxlcdbqi 66 14 vesicular vesicular JJ cord-104239-xxlcdbqi 66 15 stomafitis stomafitis NNP cord-104239-xxlcdbqi 66 16 virus virus NN cord-104239-xxlcdbqi 66 17 glycoprotein glycoprotein NN cord-104239-xxlcdbqi 66 18 ( ( -LRB- cord-104239-xxlcdbqi 66 19 VSV VSV NNP cord-104239-xxlcdbqi 66 20 - - HYPH cord-104239-xxlcdbqi 66 21 G G NNP cord-104239-xxlcdbqi 66 22 ) ) -RRB- cord-104239-xxlcdbqi 66 23 ( ( -LRB- cord-104239-xxlcdbqi 66 24 P5D4 P5D4 NNP cord-104239-xxlcdbqi 66 25 ) ) -RRB- cord-104239-xxlcdbqi 66 26 was be VBD cord-104239-xxlcdbqi 66 27 provided provide VBN cord-104239-xxlcdbqi 66 28 by by IN cord-104239-xxlcdbqi 66 29 T. T. NNP cord-104239-xxlcdbqi 66 30 Kreis Kreis NNP cord-104239-xxlcdbqi 66 31 ( ( -LRB- cord-104239-xxlcdbqi 66 32 University University NNP cord-104239-xxlcdbqi 66 33 of of IN cord-104239-xxlcdbqi 66 34 Geneva Geneva NNP cord-104239-xxlcdbqi 66 35 , , , cord-104239-xxlcdbqi 66 36 Switzerland Switzerland NNP cord-104239-xxlcdbqi 66 37 ) ) -RRB- cord-104239-xxlcdbqi 66 38 ( ( -LRB- cord-104239-xxlcdbqi 66 39 Kreis Kreis NNP cord-104239-xxlcdbqi 66 40 , , , cord-104239-xxlcdbqi 66 41 1986 1986 CD cord-104239-xxlcdbqi 66 42 ) ) -RRB- cord-104239-xxlcdbqi 66 43 . . . cord-104239-xxlcdbqi 67 1 Secondary secondary JJ cord-104239-xxlcdbqi 67 2 antibodies antibody NNS cord-104239-xxlcdbqi 67 3 were be VBD cord-104239-xxlcdbqi 67 4 obtained obtain VBN cord-104239-xxlcdbqi 67 5 from from IN cord-104239-xxlcdbqi 67 6 the the DT cord-104239-xxlcdbqi 67 7 following follow VBG cord-104239-xxlcdbqi 67 8 sources source NNS cord-104239-xxlcdbqi 67 9 : : : cord-104239-xxlcdbqi 67 10 Texas Texas NNP cord-104239-xxlcdbqi 67 11 red red JJ cord-104239-xxlcdbqi 67 12 -- -- : cord-104239-xxlcdbqi 67 13 conjugated conjugated JJ cord-104239-xxlcdbqi 67 14 goat goat NN cord-104239-xxlcdbqi 67 15 anti anti JJ cord-104239-xxlcdbqi 67 16 - - JJ cord-104239-xxlcdbqi 67 17 mouse mouse JJ cord-104239-xxlcdbqi 67 18 IgG igg NN cord-104239-xxlcdbqi 67 19 from from IN cord-104239-xxlcdbqi 67 20 Zymed Zymed NNP cord-104239-xxlcdbqi 67 21 Laboratories Laboratories NNP cord-104239-xxlcdbqi 67 22 ( ( -LRB- cord-104239-xxlcdbqi 67 23 South South NNP cord-104239-xxlcdbqi 67 24 San San NNP cord-104239-xxlcdbqi 67 25 Francisco Francisco NNP cord-104239-xxlcdbqi 67 26 , , , cord-104239-xxlcdbqi 67 27 CA CA NNP cord-104239-xxlcdbqi 67 28 ) ) -RRB- cord-104239-xxlcdbqi 67 29 , , , cord-104239-xxlcdbqi 67 30 6 6 CD cord-104239-xxlcdbqi 67 31 nm nm NN cord-104239-xxlcdbqi 67 32 gold gold NN cord-104239-xxlcdbqi 67 33 -- -- : cord-104239-xxlcdbqi 67 34 conjugated conjugate VBN cord-104239-xxlcdbqi 67 35 goat goat NN cord-104239-xxlcdbqi 67 36 anti anti JJ cord-104239-xxlcdbqi 67 37 - - JJ cord-104239-xxlcdbqi 67 38 mouse mouse JJ cord-104239-xxlcdbqi 67 39 and and CC cord-104239-xxlcdbqi 67 40 anti anti JJ cord-104239-xxlcdbqi 67 41 - - JJ cord-104239-xxlcdbqi 67 42 rabbit rabbit JJ cord-104239-xxlcdbqi 67 43 antibodies antibody NNS cord-104239-xxlcdbqi 67 44 from from IN cord-104239-xxlcdbqi 67 45 Jackson Jackson NNP cord-104239-xxlcdbqi 67 46 Immunoresearch Immunoresearch NNP cord-104239-xxlcdbqi 67 47 Laboratories Laboratories NNP cord-104239-xxlcdbqi 67 48 ( ( -LRB- cord-104239-xxlcdbqi 67 49 West West NNP cord-104239-xxlcdbqi 67 50 Groven Groven NNP cord-104239-xxlcdbqi 67 51 , , , cord-104239-xxlcdbqi 67 52 PA PA NNP cord-104239-xxlcdbqi 67 53 ) ) -RRB- cord-104239-xxlcdbqi 67 54 , , , cord-104239-xxlcdbqi 67 55 and and CC cord-104239-xxlcdbqi 67 56 10 10 CD cord-104239-xxlcdbqi 67 57 nm nm NN cord-104239-xxlcdbqi 67 58 gold gold NN cord-104239-xxlcdbqi 67 59 -- -- : cord-104239-xxlcdbqi 67 60 conjugated conjugate VBN cord-104239-xxlcdbqi 67 61 goat goat NN cord-104239-xxlcdbqi 67 62 anti anti JJ cord-104239-xxlcdbqi 67 63 - - JJ cord-104239-xxlcdbqi 67 64 mouse mouse JJ cord-104239-xxlcdbqi 67 65 antibodies antibody NNS cord-104239-xxlcdbqi 67 66 from from IN cord-104239-xxlcdbqi 67 67 Amersham Amersham NNP cord-104239-xxlcdbqi 67 68 Corp. Corp. NNP cord-104239-xxlcdbqi 67 69 ( ( -LRB- cord-104239-xxlcdbqi 67 70 Arlington Arlington NNP cord-104239-xxlcdbqi 67 71 Heights Heights NNP cord-104239-xxlcdbqi 67 72 , , , cord-104239-xxlcdbqi 67 73 IL IL NNP cord-104239-xxlcdbqi 67 74 ) ) -RRB- cord-104239-xxlcdbqi 67 75 . . . cord-104239-xxlcdbqi 68 1 Sarl Sarl NNP cord-104239-xxlcdbqi 68 2 wild wild JJ cord-104239-xxlcdbqi 68 3 type type NN cord-104239-xxlcdbqi 68 4 and and CC cord-104239-xxlcdbqi 68 5 the the DT cord-104239-xxlcdbqi 68 6 H79 H79 NNP cord-104239-xxlcdbqi 68 7 G G NNP cord-104239-xxlcdbqi 68 8 and and CC cord-104239-xxlcdbqi 68 9 T39N t39n RB cord-104239-xxlcdbqi 68 10 mutant mutant JJ cord-104239-xxlcdbqi 68 11 proteins protein NNS cord-104239-xxlcdbqi 68 12 were be VBD cord-104239-xxlcdbqi 68 13 prepared prepare VBN cord-104239-xxlcdbqi 68 14 as as IN cord-104239-xxlcdbqi 68 15 described describe VBN cord-104239-xxlcdbqi 68 16 ( ( -LRB- cord-104239-xxlcdbqi 68 17 Aridor Aridor NNP cord-104239-xxlcdbqi 68 18 et et NNP cord-104239-xxlcdbqi 68 19 al al NNP cord-104239-xxlcdbqi 68 20 . . NNP cord-104239-xxlcdbqi 68 21 , , , cord-104239-xxlcdbqi 68 22 1995 1995 CD cord-104239-xxlcdbqi 68 23 ) ) -RRB- cord-104239-xxlcdbqi 68 24 . . . cord-104239-xxlcdbqi 69 1 All all DT cord-104239-xxlcdbqi 69 2 other other JJ cord-104239-xxlcdbqi 69 3 reagents reagent NNS cord-104239-xxlcdbqi 69 4 , , , cord-104239-xxlcdbqi 69 5 except except IN cord-104239-xxlcdbqi 69 6 where where WRB cord-104239-xxlcdbqi 69 7 indicated indicate VBN cord-104239-xxlcdbqi 69 8 , , , cord-104239-xxlcdbqi 69 9 were be VBD cord-104239-xxlcdbqi 69 10 obtained obtain VBN cord-104239-xxlcdbqi 69 11 from from IN cord-104239-xxlcdbqi 69 12 Sigma Sigma NNP cord-104239-xxlcdbqi 69 13 Chemical Chemical NNP cord-104239-xxlcdbqi 69 14 Co. Co. NNP cord-104239-xxlcdbqi 69 15 ( ( -LRB- cord-104239-xxlcdbqi 69 16 St. St. NNP cord-104239-xxlcdbqi 69 17 Louis Louis NNP cord-104239-xxlcdbqi 69 18 , , , cord-104239-xxlcdbqi 69 19 MO MO NNP cord-104239-xxlcdbqi 69 20 ) ) -RRB- cord-104239-xxlcdbqi 69 21 . . . cord-104239-xxlcdbqi 70 1 Normal normal JJ cord-104239-xxlcdbqi 70 2 rat rat NN cord-104239-xxlcdbqi 70 3 kidney kidney NN cord-104239-xxlcdbqi 70 4 ( ( -LRB- cord-104239-xxlcdbqi 70 5 NRK NRK NNP cord-104239-xxlcdbqi 70 6 ) ) -RRB- cord-104239-xxlcdbqi 70 7 and and CC cord-104239-xxlcdbqi 70 8 rat rat NNP cord-104239-xxlcdbqi 70 9 basophilic basophilic NNP cord-104239-xxlcdbqi 70 10 leukemia leukemia NN cord-104239-xxlcdbqi 70 11 ( ( -LRB- cord-104239-xxlcdbqi 70 12 RBL RBL NNP cord-104239-xxlcdbqi 70 13 ) ) -RRB- cord-104239-xxlcdbqi 70 14 cells cell NNS cord-104239-xxlcdbqi 70 15 ( ( -LRB- cord-104239-xxlcdbqi 70 16 RBL-2H3 rbl-2h3 NN cord-104239-xxlcdbqi 70 17 ) ) -RRB- cord-104239-xxlcdbqi 70 18 were be VBD cord-104239-xxlcdbqi 70 19 maintained maintain VBN cord-104239-xxlcdbqi 70 20 in in IN cord-104239-xxlcdbqi 70 21 monolayer monolayer NN cord-104239-xxlcdbqi 70 22 culture culture NN cord-104239-xxlcdbqi 70 23 in in IN cord-104239-xxlcdbqi 70 24 a a DT cord-104239-xxlcdbqi 70 25 - - HYPH cord-104239-xxlcdbqi 70 26 MEM mem NN cord-104239-xxlcdbqi 70 27 supplemented supplement VBN cord-104239-xxlcdbqi 70 28 with with IN cord-104239-xxlcdbqi 70 29 penicillin penicillin NN cord-104239-xxlcdbqi 70 30 , , , cord-104239-xxlcdbqi 70 31 streptomycin streptomycin NNS cord-104239-xxlcdbqi 70 32 , , , cord-104239-xxlcdbqi 70 33 and and CC cord-104239-xxlcdbqi 70 34 7 7 CD cord-104239-xxlcdbqi 70 35 % % NN cord-104239-xxlcdbqi 70 36 ( ( -LRB- cord-104239-xxlcdbqi 70 37 NRK NRK NNP cord-104239-xxlcdbqi 70 38 ) ) -RRB- cord-104239-xxlcdbqi 70 39 or or CC cord-104239-xxlcdbqi 70 40 16 16 CD cord-104239-xxlcdbqi 70 41 % % NN cord-104239-xxlcdbqi 70 42 ( ( -LRB- cord-104239-xxlcdbqi 70 43 RBL RBL NNP cord-104239-xxlcdbqi 70 44 ) ) -RRB- cord-104239-xxlcdbqi 70 45 FBS FBS NNP cord-104239-xxlcdbqi 70 46 ( ( -LRB- cord-104239-xxlcdbqi 70 47 Gemini Gemini NNP cord-104239-xxlcdbqi 70 48 Bioproducts Bioproducts NNP cord-104239-xxlcdbqi 70 49 , , , cord-104239-xxlcdbqi 70 50 Calabasas Calabasas NNP cord-104239-xxlcdbqi 70 51 , , , cord-104239-xxlcdbqi 70 52 CA CA NNP cord-104239-xxlcdbqi 70 53 ) ) -RRB- cord-104239-xxlcdbqi 70 54 as as IN cord-104239-xxlcdbqi 70 55 described describe VBN cord-104239-xxlcdbqi 70 56 ( ( -LRB- cord-104239-xxlcdbqi 70 57 Plutner Plutner NNP cord-104239-xxlcdbqi 70 58 eta eta NNP cord-104239-xxlcdbqi 70 59 ] ] -RRB- cord-104239-xxlcdbqi 70 60 . . . cord-104239-xxlcdbqi 71 1 , , , cord-104239-xxlcdbqi 71 2 1992 1992 CD cord-104239-xxlcdbqi 71 3 ) ) -RRB- cord-104239-xxlcdbqi 71 4 . . . cord-104239-xxlcdbqi 72 1 Ts045-VSV Ts045-VSV NNPS cord-104239-xxlcdbqi 73 1 ( ( -LRB- cord-104239-xxlcdbqi 73 2 Lafay Lafay NNP cord-104239-xxlcdbqi 73 3 , , , cord-104239-xxlcdbqi 73 4 1974 1974 CD cord-104239-xxlcdbqi 73 5 ) ) -RRB- cord-104239-xxlcdbqi 73 6 was be VBD cord-104239-xxlcdbqi 73 7 propagated propagate VBN cord-104239-xxlcdbqi 73 8 in in IN cord-104239-xxlcdbqi 73 9 BHK-21 BHK-21 NNP cord-104239-xxlcdbqi 73 10 ceils ceil NNS cord-104239-xxlcdbqi 73 11 as as IN cord-104239-xxlcdbqi 73 12 described describe VBN cord-104239-xxlcdbqi 73 13 ( ( -LRB- cord-104239-xxlcdbqi 73 14 Beckers Beckers NNP cord-104239-xxlcdbqi 73 15 et et NNP cord-104239-xxlcdbqi 73 16 al al NNP cord-104239-xxlcdbqi 73 17 . . NNP cord-104239-xxlcdbqi 73 18 , , , cord-104239-xxlcdbqi 73 19 1987 1987 CD cord-104239-xxlcdbqi 73 20 ) ) -RRB- cord-104239-xxlcdbqi 73 21 . . . cord-104239-xxlcdbqi 74 1 Cells cell NNS cord-104239-xxlcdbqi 74 2 were be VBD cord-104239-xxlcdbqi 74 3 infected infect VBN cord-104239-xxlcdbqi 74 4 with with IN cord-104239-xxlcdbqi 74 5 ts045-VSV ts045-VSV NNS cord-104239-xxlcdbqi 74 6 at at IN cord-104239-xxlcdbqi 74 7 a a DT cord-104239-xxlcdbqi 74 8 multiplicity multiplicity NN cord-104239-xxlcdbqi 74 9 of of IN cord-104239-xxlcdbqi 74 10 10 10 CD cord-104239-xxlcdbqi 74 11 - - SYM cord-104239-xxlcdbqi 74 12 20 20 CD cord-104239-xxlcdbqi 74 13 plaque plaque NN cord-104239-xxlcdbqi 74 14 - - HYPH cord-104239-xxlcdbqi 74 15 forming form VBG cord-104239-xxlcdbqi 74 16 units unit NNS cord-104239-xxlcdbqi 74 17 per per IN cord-104239-xxlcdbqi 74 18 cell cell NN cord-104239-xxlcdbqi 74 19 as as IN cord-104239-xxlcdbqi 74 20 described describe VBN cord-104239-xxlcdbqi 74 21 ( ( -LRB- cord-104239-xxlcdbqi 74 22 Davidson Davidson NNP cord-104239-xxlcdbqi 74 23 and and CC cord-104239-xxlcdbqi 74 24 Balch Balch NNP cord-104239-xxlcdbqi 74 25 , , , cord-104239-xxlcdbqi 74 26 1993 1993 CD cord-104239-xxlcdbqi 74 27 ) ) -RRB- cord-104239-xxlcdbqi 74 28 . . . cord-104239-xxlcdbqi 75 1 Cells cell NNS cord-104239-xxlcdbqi 75 2 were be VBD cord-104239-xxlcdbqi 75 3 fixed fix VBN cord-104239-xxlcdbqi 75 4 in in IN cord-104239-xxlcdbqi 75 5 2.5 2.5 CD cord-104239-xxlcdbqi 75 6 % % NN cord-104239-xxlcdbqi 75 7 glutaraldehyde glutaraldehyde NN cord-104239-xxlcdbqi 75 8 ( ( -LRB- cord-104239-xxlcdbqi 75 9 GA GA NNP cord-104239-xxlcdbqi 75 10 ) ) -RRB- cord-104239-xxlcdbqi 75 11 in in IN cord-104239-xxlcdbqi 75 12 PBS PBS NNP cord-104239-xxlcdbqi 75 13 for for IN cord-104239-xxlcdbqi 75 14 1 1 CD cord-104239-xxlcdbqi 75 15 h h NN cord-104239-xxlcdbqi 75 16 at at IN cord-104239-xxlcdbqi 75 17 room room NN cord-104239-xxlcdbqi 75 18 temperature temperature NN cord-104239-xxlcdbqi 75 19 ( ( -LRB- cord-104239-xxlcdbqi 75 20 rt rt NNP cord-104239-xxlcdbqi 75 21 ) ) -RRB- cord-104239-xxlcdbqi 75 22 , , , cord-104239-xxlcdbqi 75 23 scraped scrape VBN cord-104239-xxlcdbqi 75 24 in in IN cord-104239-xxlcdbqi 75 25 GA GA NNP cord-104239-xxlcdbqi 75 26 , , , cord-104239-xxlcdbqi 75 27 and and CC cord-104239-xxlcdbqi 75 28 pelleted pellete VBD cord-104239-xxlcdbqi 75 29 at at IN cord-104239-xxlcdbqi 75 30 15,000 15,000 CD cord-104239-xxlcdbqi 75 31 g g NN cord-104239-xxlcdbqi 75 32 in in IN cord-104239-xxlcdbqi 75 33 a a DT cord-104239-xxlcdbqi 75 34 microcentrifuge microcentrifuge NN cord-104239-xxlcdbqi 75 35 for for IN cord-104239-xxlcdbqi 75 36 10 10 CD cord-104239-xxlcdbqi 75 37 rain rain NN cord-104239-xxlcdbqi 75 38 . . . cord-104239-xxlcdbqi 76 1 The the DT cord-104239-xxlcdbqi 76 2 tight tight JJ cord-104239-xxlcdbqi 76 3 pellet pellet NN cord-104239-xxlcdbqi 76 4 was be VBD cord-104239-xxlcdbqi 76 5 washed wash VBN cord-104239-xxlcdbqi 76 6 in in IN cord-104239-xxlcdbqi 76 7 veronal veronal JJ cord-104239-xxlcdbqi 76 8 - - HYPH cord-104239-xxlcdbqi 76 9 acetate acetate NN cord-104239-xxlcdbqi 76 10 buffer buffer NN cord-104239-xxlcdbqi 76 11 ( ( -LRB- cord-104239-xxlcdbqi 76 12 pH pH NNP cord-104239-xxlcdbqi 76 13 6.0 6.0 CD cord-104239-xxlcdbqi 76 14 ) ) -RRB- cord-104239-xxlcdbqi 76 15 and and CC cord-104239-xxlcdbqi 76 16 stained stain VBN cord-104239-xxlcdbqi 76 17 in in IN cord-104239-xxlcdbqi 76 18 1 1 CD cord-104239-xxlcdbqi 76 19 % % NN cord-104239-xxlcdbqi 76 20 buffered buffer VBN cord-104239-xxlcdbqi 76 21 OsO4 oso4 RB cord-104239-xxlcdbqi 76 22 for for IN cord-104239-xxlcdbqi 76 23 1 1 CD cord-104239-xxlcdbqi 76 24 h h NN cord-104239-xxlcdbqi 76 25 at at IN cord-104239-xxlcdbqi 76 26 rt rt NNP cord-104239-xxlcdbqi 76 27 . . . cord-104239-xxlcdbqi 77 1 After after IN cord-104239-xxlcdbqi 77 2 washing wash VBG cord-104239-xxlcdbqi 77 3 in in IN cord-104239-xxlcdbqi 77 4 veronalacetate veronalacetate NN cord-104239-xxlcdbqi 77 5 buffer buffer NN cord-104239-xxlcdbqi 77 6 , , , cord-104239-xxlcdbqi 77 7 pellets pellet NNS cord-104239-xxlcdbqi 77 8 were be VBD cord-104239-xxlcdbqi 77 9 stained stain VBN cord-104239-xxlcdbqi 77 10 en en IN cord-104239-xxlcdbqi 77 11 bloc bloc NN cord-104239-xxlcdbqi 77 12 with with IN cord-104239-xxlcdbqi 77 13 2 2 CD cord-104239-xxlcdbqi 77 14 % % NN cord-104239-xxlcdbqi 77 15 uranyl uranyl NN cord-104239-xxlcdbqi 77 16 acetate acetate NN cord-104239-xxlcdbqi 77 17 in in IN cord-104239-xxlcdbqi 77 18 veronal veronal JJ cord-104239-xxlcdbqi 77 19 - - HYPH cord-104239-xxlcdbqi 77 20 acetate acetate NN cord-104239-xxlcdbqi 77 21 buffer buffer NN cord-104239-xxlcdbqi 77 22 , , , cord-104239-xxlcdbqi 77 23 dehydrated dehydrate VBN cord-104239-xxlcdbqi 77 24 in in IN cord-104239-xxlcdbqi 77 25 alcohol alcohol NN cord-104239-xxlcdbqi 77 26 and and CC cord-104239-xxlcdbqi 77 27 acetone acetone NN cord-104239-xxlcdbqi 77 28 , , , cord-104239-xxlcdbqi 77 29 and and CC cord-104239-xxlcdbqi 77 30 embedded embed VBN cord-104239-xxlcdbqi 77 31 in in IN cord-104239-xxlcdbqi 77 32 Epon Epon NNP cord-104239-xxlcdbqi 77 33 812 812 CD cord-104239-xxlcdbqi 78 1 ( ( -LRB- cord-104239-xxlcdbqi 78 2 Electron Electron NNP cord-104239-xxlcdbqi 78 3 Microscopy Microscopy NNP cord-104239-xxlcdbqi 78 4 , , , cord-104239-xxlcdbqi 78 5 Sciences Sciences NNPS cord-104239-xxlcdbqi 78 6 , , , cord-104239-xxlcdbqi 78 7 Fort Fort NNP cord-104239-xxlcdbqi 78 8 Washington Washington NNP cord-104239-xxlcdbqi 78 9 , , , cord-104239-xxlcdbqi 78 10 PA PA NNP cord-104239-xxlcdbqi 78 11 ) ) -RRB- cord-104239-xxlcdbqi 78 12 . . . cord-104239-xxlcdbqi 79 1 For for IN cord-104239-xxlcdbqi 79 2 serial serial JJ cord-104239-xxlcdbqi 79 3 sectioning sectioning NN cord-104239-xxlcdbqi 79 4 , , , cord-104239-xxlcdbqi 79 5 the the DT cord-104239-xxlcdbqi 79 6 cell cell NN cord-104239-xxlcdbqi 79 7 pellet pellet NN cord-104239-xxlcdbqi 79 8 was be VBD cord-104239-xxlcdbqi 79 9 cut cut VBN cord-104239-xxlcdbqi 79 10 with with IN cord-104239-xxlcdbqi 79 11 a a DT cord-104239-xxlcdbqi 79 12 glass glass NN cord-104239-xxlcdbqi 79 13 knife knife NN cord-104239-xxlcdbqi 79 14 followed follow VBN cord-104239-xxlcdbqi 79 15 by by IN cord-104239-xxlcdbqi 79 16 trimming trim VBG cord-104239-xxlcdbqi 79 17 of of IN cord-104239-xxlcdbqi 79 18 the the DT cord-104239-xxlcdbqi 79 19 resulting result VBG cord-104239-xxlcdbqi 79 20 block block NN cord-104239-xxlcdbqi 79 21 to to TO cord-104239-xxlcdbqi 79 22 obtain obtain VB cord-104239-xxlcdbqi 79 23 an an DT cord-104239-xxlcdbqi 79 24 ~40 ~40 XX cord-104239-xxlcdbqi 79 25 ×300 ×300 NFP cord-104239-xxlcdbqi 79 26 ~xm ~xm NNP cord-104239-xxlcdbqi 79 27 pyramid pyramid NN cord-104239-xxlcdbqi 79 28 . . . cord-104239-xxlcdbqi 80 1 A a DT cord-104239-xxlcdbqi 80 2 ribbon ribbon NN cord-104239-xxlcdbqi 80 3 of of IN cord-104239-xxlcdbqi 80 4 25 25 CD cord-104239-xxlcdbqi 80 5 - - SYM cord-104239-xxlcdbqi 80 6 35 35 CD cord-104239-xxlcdbqi 80 7 consecutive consecutive JJ cord-104239-xxlcdbqi 80 8 sections section NNS cord-104239-xxlcdbqi 80 9 of of IN cord-104239-xxlcdbqi 80 10 65 65 CD cord-104239-xxlcdbqi 80 11 nm nm NN cord-104239-xxlcdbqi 80 12 in in IN cord-104239-xxlcdbqi 80 13 thickness thickness NN cord-104239-xxlcdbqi 80 14 was be VBD cord-104239-xxlcdbqi 80 15 cut cut VBN cord-104239-xxlcdbqi 80 16 with with IN cord-104239-xxlcdbqi 80 17 a a DT cord-104239-xxlcdbqi 80 18 diamond diamond NN cord-104239-xxlcdbqi 80 19 knife knife NN cord-104239-xxlcdbqi 80 20 on on IN cord-104239-xxlcdbqi 80 21 Reichert Reichert NNP cord-104239-xxlcdbqi 80 22 ultramicrotome ultramicrotome NNP cord-104239-xxlcdbqi 80 23 2E 2E NNP cord-104239-xxlcdbqi 80 24 and and CC cord-104239-xxlcdbqi 80 25 transferred transfer VBN cord-104239-xxlcdbqi 80 26 to to IN cord-104239-xxlcdbqi 80 27 a a DT cord-104239-xxlcdbqi 80 28 single single JJ cord-104239-xxlcdbqi 80 29 2 2 CD cord-104239-xxlcdbqi 80 30 × × NN cord-104239-xxlcdbqi 80 31 1.5 1.5 CD cord-104239-xxlcdbqi 80 32 mm mm NN cord-104239-xxlcdbqi 80 33 slot slot NN cord-104239-xxlcdbqi 80 34 grid grid NN cord-104239-xxlcdbqi 80 35 ( ( -LRB- cord-104239-xxlcdbqi 80 36 Electron Electron NNP cord-104239-xxlcdbqi 80 37 Microscopy Microscopy NNP cord-104239-xxlcdbqi 80 38 Sciences Sciences NNPS cord-104239-xxlcdbqi 80 39 ) ) -RRB- cord-104239-xxlcdbqi 80 40 precoated precoate VBD cord-104239-xxlcdbqi 80 41 with with IN cord-104239-xxlcdbqi 80 42 a a DT cord-104239-xxlcdbqi 80 43 Formvar Formvar NNP cord-104239-xxlcdbqi 80 44 / / SYM cord-104239-xxlcdbqi 80 45 carbon carbon NN cord-104239-xxlcdbqi 80 46 film film NN cord-104239-xxlcdbqi 80 47 . . . cord-104239-xxlcdbqi 81 1 Sections section NNS cord-104239-xxlcdbqi 81 2 were be VBD cord-104239-xxlcdbqi 81 3 counterstained counterstaine VBN cord-104239-xxlcdbqi 81 4 with with IN cord-104239-xxlcdbqi 81 5 a a DT cord-104239-xxlcdbqi 81 6 saturated saturated JJ cord-104239-xxlcdbqi 81 7 solution solution NN cord-104239-xxlcdbqi 81 8 of of IN cord-104239-xxlcdbqi 81 9 uranyl uranyl NN cord-104239-xxlcdbqi 81 10 acetate acetate NN cord-104239-xxlcdbqi 81 11 in in IN cord-104239-xxlcdbqi 81 12 methanol methanol NN cord-104239-xxlcdbqi 81 13 for for IN cord-104239-xxlcdbqi 81 14 10 10 CD cord-104239-xxlcdbqi 81 15 rain rain NN cord-104239-xxlcdbqi 81 16 at at IN cord-104239-xxlcdbqi 81 17 rt rt NNP cord-104239-xxlcdbqi 81 18 and and CC cord-104239-xxlcdbqi 81 19 Reynold Reynold NNP cord-104239-xxlcdbqi 81 20 's 's POS cord-104239-xxlcdbqi 81 21 lead lead NN cord-104239-xxlcdbqi 81 22 citrate citrate NN cord-104239-xxlcdbqi 81 23 for for IN cord-104239-xxlcdbqi 81 24 10 10 CD cord-104239-xxlcdbqi 81 25 min min NN cord-104239-xxlcdbqi 81 26 . . . cord-104239-xxlcdbqi 82 1 Budding bud VBG cord-104239-xxlcdbqi 82 2 structures structure NNS cord-104239-xxlcdbqi 82 3 on on IN cord-104239-xxlcdbqi 82 4 the the DT cord-104239-xxlcdbqi 82 5 ER er NN cord-104239-xxlcdbqi 82 6 and and CC cord-104239-xxlcdbqi 82 7 VTCs vtc NNS cord-104239-xxlcdbqi 82 8 were be VBD cord-104239-xxlcdbqi 82 9 followed follow VBN cord-104239-xxlcdbqi 82 10 in in IN cord-104239-xxlcdbqi 82 11 consecutive consecutive JJ cord-104239-xxlcdbqi 82 12 sections section NNS cord-104239-xxlcdbqi 82 13 , , , cord-104239-xxlcdbqi 82 14 and and CC cord-104239-xxlcdbqi 82 15 images image NNS cord-104239-xxlcdbqi 82 16 were be VBD cord-104239-xxlcdbqi 82 17 overlaid overlay VBN cord-104239-xxlcdbqi 82 18 to to TO cord-104239-xxlcdbqi 82 19 reconstruct reconstruct VB cord-104239-xxlcdbqi 82 20 continuity continuity NN cord-104239-xxlcdbqi 82 21 between between IN cord-104239-xxlcdbqi 82 22 structures structure NNS cord-104239-xxlcdbqi 82 23 . . . cord-104239-xxlcdbqi 83 1 Stereological Stereological NNP cord-104239-xxlcdbqi 83 2 Parameters parameter NNS cord-104239-xxlcdbqi 83 3 . . . cord-104239-xxlcdbqi 84 1 An an DT cord-104239-xxlcdbqi 84 2 estimation estimation NN cord-104239-xxlcdbqi 84 3 of of IN cord-104239-xxlcdbqi 84 4 the the DT cord-104239-xxlcdbqi 84 5 mean mean JJ cord-104239-xxlcdbqi 84 6 volume volume NN cord-104239-xxlcdbqi 84 7 of of IN cord-104239-xxlcdbqi 84 8 the the DT cord-104239-xxlcdbqi 84 9 cells cell NNS cord-104239-xxlcdbqi 84 10 was be VBD cord-104239-xxlcdbqi 84 11 performed perform VBN cord-104239-xxlcdbqi 84 12 by by IN cord-104239-xxlcdbqi 84 13 two two CD cord-104239-xxlcdbqi 84 14 independent independent JJ cord-104239-xxlcdbqi 84 15 methods method NNS cord-104239-xxlcdbqi 84 16 ( ( -LRB- cord-104239-xxlcdbqi 84 17 Baddeley Baddeley NNP cord-104239-xxlcdbqi 84 18 et et NNP cord-104239-xxlcdbqi 84 19 al al NNP cord-104239-xxlcdbqi 84 20 . . NNP cord-104239-xxlcdbqi 84 21 , , , cord-104239-xxlcdbqi 84 22 1985 1985 CD cord-104239-xxlcdbqi 84 23 ; ; : cord-104239-xxlcdbqi 84 24 Griffiths Griffiths NNP cord-104239-xxlcdbqi 84 25 et et NNP cord-104239-xxlcdbqi 84 26 al al NNP cord-104239-xxlcdbqi 84 27 . . NNP cord-104239-xxlcdbqi 84 28 , , , cord-104239-xxlcdbqi 84 29 1989 1989 CD cord-104239-xxlcdbqi 84 30 ) ) -RRB- cord-104239-xxlcdbqi 84 31 . . . cord-104239-xxlcdbqi 85 1 In in IN cord-104239-xxlcdbqi 85 2 the the DT cord-104239-xxlcdbqi 85 3 first first JJ cord-104239-xxlcdbqi 85 4 case case NN cord-104239-xxlcdbqi 85 5 , , , cord-104239-xxlcdbqi 85 6 RBL rbl NN cord-104239-xxlcdbqi 85 7 cells cell NNS cord-104239-xxlcdbqi 85 8 were be VBD cord-104239-xxlcdbqi 85 9 grown grow VBN cord-104239-xxlcdbqi 85 10 on on IN cord-104239-xxlcdbqi 85 11 glass glass NN cord-104239-xxlcdbqi 85 12 coverslips coverslip NNS cord-104239-xxlcdbqi 85 13 . . . cord-104239-xxlcdbqi 86 1 Cells cell NNS cord-104239-xxlcdbqi 86 2 were be VBD cord-104239-xxlcdbqi 86 3 fixed fix VBN cord-104239-xxlcdbqi 86 4 with with IN cord-104239-xxlcdbqi 86 5 2.5 2.5 CD cord-104239-xxlcdbqi 86 6 % % NN cord-104239-xxlcdbqi 86 7 GA GA NNP cord-104239-xxlcdbqi 86 8 in in IN cord-104239-xxlcdbqi 86 9 PBS PBS NNP cord-104239-xxlcdbqi 86 10 , , , cord-104239-xxlcdbqi 86 11 pH pH NNP cord-104239-xxlcdbqi 86 12 7.4 7.4 CD cord-104239-xxlcdbqi 86 13 , , , cord-104239-xxlcdbqi 86 14 overnight overnight RB cord-104239-xxlcdbqi 86 15 . . . cord-104239-xxlcdbqi 87 1 The the DT cord-104239-xxlcdbqi 87 2 cells cell NNS cord-104239-xxlcdbqi 87 3 were be VBD cord-104239-xxlcdbqi 87 4 then then RB cord-104239-xxlcdbqi 87 5 viewed view VBN cord-104239-xxlcdbqi 87 6 and and CC cord-104239-xxlcdbqi 87 7 photographed photograph VBN cord-104239-xxlcdbqi 87 8 under under IN cord-104239-xxlcdbqi 87 9 phase phase NN cord-104239-xxlcdbqi 87 10 contrast contrast NN cord-104239-xxlcdbqi 87 11 with with IN cord-104239-xxlcdbqi 87 12 an an DT cord-104239-xxlcdbqi 87 13 Axiophot Axiophot NNP cord-104239-xxlcdbqi 87 14 ( ( -LRB- cord-104239-xxlcdbqi 87 15 Zeiss Zeiss NNP cord-104239-xxlcdbqi 87 16 , , , cord-104239-xxlcdbqi 87 17 Oberkochen Oberkochen NNP cord-104239-xxlcdbqi 87 18 , , , cord-104239-xxlcdbqi 87 19 Germany Germany NNP cord-104239-xxlcdbqi 87 20 ) ) -RRB- cord-104239-xxlcdbqi 87 21 with with IN cord-104239-xxlcdbqi 87 22 a a DT cord-104239-xxlcdbqi 87 23 ×20 ×20 CD cord-104239-xxlcdbqi 87 24 objective objective JJ cord-104239-xxlcdbqi 87 25 lens lens NN cord-104239-xxlcdbqi 87 26 . . . cord-104239-xxlcdbqi 88 1 The the DT cord-104239-xxlcdbqi 88 2 images image NNS cord-104239-xxlcdbqi 88 3 were be VBD cord-104239-xxlcdbqi 88 4 enlarged enlarge VBN cord-104239-xxlcdbqi 88 5 photographically photographically RB cord-104239-xxlcdbqi 88 6 10 10 CD cord-104239-xxlcdbqi 88 7 times time NNS cord-104239-xxlcdbqi 88 8 , , , cord-104239-xxlcdbqi 88 9 and and CC cord-104239-xxlcdbqi 88 10 the the DT cord-104239-xxlcdbqi 88 11 mean mean JJ cord-104239-xxlcdbqi 88 12 surface surface NN cord-104239-xxlcdbqi 88 13 of of IN cord-104239-xxlcdbqi 88 14 the the DT cord-104239-xxlcdbqi 88 15 cell cell NN cord-104239-xxlcdbqi 88 16 projection projection NN cord-104239-xxlcdbqi 88 17 was be VBD cord-104239-xxlcdbqi 88 18 determined determine VBN cord-104239-xxlcdbqi 88 19 by by IN cord-104239-xxlcdbqi 88 20 the the DT cord-104239-xxlcdbqi 88 21 point point NN cord-104239-xxlcdbqi 88 22 - - HYPH cord-104239-xxlcdbqi 88 23 counting counting NN cord-104239-xxlcdbqi 88 24 method method NN cord-104239-xxlcdbqi 88 25 ( ( -LRB- cord-104239-xxlcdbqi 88 26 Weibel Weibel NNP cord-104239-xxlcdbqi 88 27 , , , cord-104239-xxlcdbqi 88 28 1979 1979 CD cord-104239-xxlcdbqi 88 29 ) ) -RRB- cord-104239-xxlcdbqi 88 30 using use VBG cord-104239-xxlcdbqi 88 31 a a DT cord-104239-xxlcdbqi 88 32 5-ram 5-ram JJ cord-104239-xxlcdbqi 88 33 square square JJ cord-104239-xxlcdbqi 88 34 lattice lattice NN cord-104239-xxlcdbqi 88 35 grid grid NN cord-104239-xxlcdbqi 88 36 . . . cord-104239-xxlcdbqi 89 1 Approximately approximately RB cord-104239-xxlcdbqi 89 2 10,000 10,000 CD cord-104239-xxlcdbqi 89 3 RBL rbl NN cord-104239-xxlcdbqi 89 4 cells cell NNS cord-104239-xxlcdbqi 89 5 were be VBD cord-104239-xxlcdbqi 89 6 used use VBN cord-104239-xxlcdbqi 89 7 for for IN cord-104239-xxlcdbqi 89 8 the the DT cord-104239-xxlcdbqi 89 9 estimation estimation NN cord-104239-xxlcdbqi 89 10 of of IN cord-104239-xxlcdbqi 89 11 the the DT cord-104239-xxlcdbqi 89 12 mean mean JJ cord-104239-xxlcdbqi 89 13 surface surface NN cord-104239-xxlcdbqi 89 14 of of IN cord-104239-xxlcdbqi 89 15 the the DT cord-104239-xxlcdbqi 89 16 cell cell NN cord-104239-xxlcdbqi 89 17 projection projection NN cord-104239-xxlcdbqi 89 18 onto onto IN cord-104239-xxlcdbqi 89 19 a a DT cord-104239-xxlcdbqi 89 20 planar planar JJ cord-104239-xxlcdbqi 89 21 surface surface NN cord-104239-xxlcdbqi 89 22 . . . cord-104239-xxlcdbqi 90 1 The the DT cord-104239-xxlcdbqi 90 2 cells cell NNS cord-104239-xxlcdbqi 90 3 on on IN cord-104239-xxlcdbqi 90 4 coverslips coverslip NNS cord-104239-xxlcdbqi 90 5 were be VBD cord-104239-xxlcdbqi 90 6 stained stain VBN cord-104239-xxlcdbqi 90 7 and and CC cord-104239-xxlcdbqi 90 8 embedded embed VBN cord-104239-xxlcdbqi 90 9 in in IN cord-104239-xxlcdbqi 90 10 Epon Epon NNP cord-104239-xxlcdbqi 90 11 as as IN cord-104239-xxlcdbqi 90 12 described describe VBN cord-104239-xxlcdbqi 90 13 above above RB cord-104239-xxlcdbqi 90 14 . . . cord-104239-xxlcdbqi 91 1 After after IN cord-104239-xxlcdbqi 91 2 polymerization polymerization NN cord-104239-xxlcdbqi 91 3 , , , cord-104239-xxlcdbqi 91 4 the the DT cord-104239-xxlcdbqi 91 5 coverslips coverslip NNS cord-104239-xxlcdbqi 91 6 were be VBD cord-104239-xxlcdbqi 91 7 detached detach VBN cord-104239-xxlcdbqi 91 8 from from IN cord-104239-xxlcdbqi 91 9 the the DT cord-104239-xxlcdbqi 91 10 resin resin NN cord-104239-xxlcdbqi 91 11 by by IN cord-104239-xxlcdbqi 91 12 plunging plunge VBG cord-104239-xxlcdbqi 91 13 into into IN cord-104239-xxlcdbqi 91 14 liquid liquid JJ cord-104239-xxlcdbqi 91 15 nitrogen nitrogen NN cord-104239-xxlcdbqi 91 16 . . . cord-104239-xxlcdbqi 92 1 Thin thin JJ cord-104239-xxlcdbqi 92 2 - - HYPH cord-104239-xxlcdbqi 92 3 strips strip NNS cord-104239-xxlcdbqi 92 4 of of IN cord-104239-xxlcdbqi 92 5 embedded embed VBN cord-104239-xxlcdbqi 92 6 cells cell NNS cord-104239-xxlcdbqi 92 7 in in IN cord-104239-xxlcdbqi 92 8 resin resin NN cord-104239-xxlcdbqi 92 9 were be VBD cord-104239-xxlcdbqi 92 10 then then RB cord-104239-xxlcdbqi 92 11 combined combine VBN cord-104239-xxlcdbqi 92 12 face face NN cord-104239-xxlcdbqi 92 13 to to IN cord-104239-xxlcdbqi 92 14 face face NN cord-104239-xxlcdbqi 92 15 and and CC cord-104239-xxlcdbqi 92 16 reembedded reembedde VBN cord-104239-xxlcdbqi 92 17 on on IN cord-104239-xxlcdbqi 92 18 top top NN cord-104239-xxlcdbqi 92 19 of of IN cord-104239-xxlcdbqi 92 20 an an DT cord-104239-xxlcdbqi 92 21 Epon Epon NNP cord-104239-xxlcdbqi 92 22 block block NN cord-104239-xxlcdbqi 92 23 to to TO cord-104239-xxlcdbqi 92 24 produce produce VB cord-104239-xxlcdbqi 92 25 " " `` cord-104239-xxlcdbqi 92 26 vertical vertical JJ cord-104239-xxlcdbqi 92 27 " " '' cord-104239-xxlcdbqi 92 28 sections section NNS cord-104239-xxlcdbqi 92 29 ( ( -LRB- cord-104239-xxlcdbqi 92 30 Griffiths Griffiths NNP cord-104239-xxlcdbqi 92 31 et et NNP cord-104239-xxlcdbqi 92 32 al al NNP cord-104239-xxlcdbqi 92 33 . . NNP cord-104239-xxlcdbqi 92 34 , , , cord-104239-xxlcdbqi 92 35 1984 1984 CD cord-104239-xxlcdbqi 92 36 ) ) -RRB- cord-104239-xxlcdbqi 92 37 . . . cord-104239-xxlcdbqi 93 1 Images image NNS cord-104239-xxlcdbqi 93 2 of of IN cord-104239-xxlcdbqi 93 3 cells cell NNS cord-104239-xxlcdbqi 93 4 were be VBD cord-104239-xxlcdbqi 93 5 taken take VBN cord-104239-xxlcdbqi 93 6 with with IN cord-104239-xxlcdbqi 93 7 a a DT cord-104239-xxlcdbqi 93 8 transmission transmission NN cord-104239-xxlcdbqi 93 9 electron electron NN cord-104239-xxlcdbqi 93 10 microscope microscope NN cord-104239-xxlcdbqi 93 11 ( ( -LRB- cord-104239-xxlcdbqi 93 12 1200EX 1200ex CD cord-104239-xxlcdbqi 93 13 ; ; : cord-104239-xxlcdbqi 93 14 JEOL JEOL NNP cord-104239-xxlcdbqi 93 15 USA USA NNP cord-104239-xxlcdbqi 93 16 , , , cord-104239-xxlcdbqi 93 17 Peabody Peabody NNP cord-104239-xxlcdbqi 93 18 , , , cord-104239-xxlcdbqi 93 19 MA MA NNP cord-104239-xxlcdbqi 93 20 ) ) -RRB- cord-104239-xxlcdbqi 93 21 and and CC cord-104239-xxlcdbqi 93 22 photoenlarged photoenlarged JJ cord-104239-xxlcdbqi 93 23 ( ( -LRB- cord-104239-xxlcdbqi 93 24 ×3,500 ×3,500 CD cord-104239-xxlcdbqi 93 25 total total JJ cord-104239-xxlcdbqi 93 26 magnification magnification NN cord-104239-xxlcdbqi 93 27 ) ) -RRB- cord-104239-xxlcdbqi 93 28 , , , cord-104239-xxlcdbqi 93 29 and and CC cord-104239-xxlcdbqi 93 30 the the DT cord-104239-xxlcdbqi 93 31 mean mean JJ cord-104239-xxlcdbqi 93 32 height height NN cord-104239-xxlcdbqi 93 33 of of IN cord-104239-xxlcdbqi 93 34 ~250 ~250 JJ cord-104239-xxlcdbqi 93 35 cells cell NNS cord-104239-xxlcdbqi 93 36 in in IN cord-104239-xxlcdbqi 93 37 a a DT cord-104239-xxlcdbqi 93 38 monolayer monolayer NN cord-104239-xxlcdbqi 93 39 was be VBD cord-104239-xxlcdbqi 93 40 measured measure VBN cord-104239-xxlcdbqi 93 41 by by IN cord-104239-xxlcdbqi 93 42 the the DT cord-104239-xxlcdbqi 93 43 point point NN cord-104239-xxlcdbqi 93 44 - - HYPH cord-104239-xxlcdbqi 93 45 counting counting NN cord-104239-xxlcdbqi 93 46 method method NN cord-104239-xxlcdbqi 93 47 ( ( -LRB- cord-104239-xxlcdbqi 93 48 Weibel Weibel NNP cord-104239-xxlcdbqi 93 49 , , , cord-104239-xxlcdbqi 93 50 1979 1979 CD cord-104239-xxlcdbqi 93 51 ) ) -RRB- cord-104239-xxlcdbqi 93 52 . . . cord-104239-xxlcdbqi 94 1 The the DT cord-104239-xxlcdbqi 94 2 mean mean JJ cord-104239-xxlcdbqi 94 3 cell cell NN cord-104239-xxlcdbqi 94 4 volume volume NN cord-104239-xxlcdbqi 94 5 ( ( -LRB- cord-104239-xxlcdbqi 94 6 Vc.r Vc.r NNP cord-104239-xxlcdbqi 94 7 . . . cord-104239-xxlcdbqi 94 8 ) ) -RRB- cord-104239-xxlcdbqi 94 9 was be VBD cord-104239-xxlcdbqi 94 10 then then RB cord-104239-xxlcdbqi 94 11 determined determine VBN cord-104239-xxlcdbqi 94 12 according accord VBG cord-104239-xxlcdbqi 94 13 to to IN cord-104239-xxlcdbqi 94 14 formula formula NN cord-104239-xxlcdbqi 94 15 : : : cord-104239-xxlcdbqi 94 16 where where WRB cord-104239-xxlcdbqi 94 17 Sc.p Sc.p NNP cord-104239-xxlcdbqi 94 18 . . . cord-104239-xxlcdbqi 94 19 is be VBZ cord-104239-xxlcdbqi 94 20 the the DT cord-104239-xxlcdbqi 94 21 mean mean JJ cord-104239-xxlcdbqi 94 22 surface surface NN cord-104239-xxlcdbqi 94 23 of of IN cord-104239-xxlcdbqi 94 24 the the DT cord-104239-xxlcdbqi 94 25 cell cell NN cord-104239-xxlcdbqi 94 26 projection projection NN cord-104239-xxlcdbqi 94 27 ( ( -LRB- cord-104239-xxlcdbqi 94 28 determined determine VBN cord-104239-xxlcdbqi 94 29 by by IN cord-104239-xxlcdbqi 94 30 the the DT cord-104239-xxlcdbqi 94 31 phase phase NN cord-104239-xxlcdbqi 94 32 - - HYPH cord-104239-xxlcdbqi 94 33 contrast contrast NN cord-104239-xxlcdbqi 94 34 light light JJ cord-104239-xxlcdbqi 94 35 microscopy microscopy NNS cord-104239-xxlcdbqi 94 36 ) ) -RRB- cord-104239-xxlcdbqi 94 37 , , , cord-104239-xxlcdbqi 94 38 and and CC cord-104239-xxlcdbqi 94 39 Hc Hc NNP cord-104239-xxlcdbqi 94 40 . . . cord-104239-xxlcdbqi 94 41 is be VBZ cord-104239-xxlcdbqi 94 42 the the DT cord-104239-xxlcdbqi 94 43 mean mean JJ cord-104239-xxlcdbqi 94 44 height height NN cord-104239-xxlcdbqi 94 45 of of IN cord-104239-xxlcdbqi 94 46 the the DT cord-104239-xxlcdbqi 94 47 cell cell NN cord-104239-xxlcdbqi 94 48 ( ( -LRB- cord-104239-xxlcdbqi 94 49 determined determine VBN cord-104239-xxlcdbqi 94 50 by by IN cord-104239-xxlcdbqi 94 51 TEM TEM NNP cord-104239-xxlcdbqi 94 52 ) ) -RRB- cord-104239-xxlcdbqi 94 53 . . . cord-104239-xxlcdbqi 95 1 For for IN cord-104239-xxlcdbqi 95 2 estimation estimation NN cord-104239-xxlcdbqi 95 3 of of IN cord-104239-xxlcdbqi 95 4 the the DT cord-104239-xxlcdbqi 95 5 mean mean JJ cord-104239-xxlcdbqi 95 6 cell cell NN cord-104239-xxlcdbqi 95 7 volume volume NN cord-104239-xxlcdbqi 95 8 using use VBG cord-104239-xxlcdbqi 95 9 the the DT cord-104239-xxlcdbqi 95 10 second second JJ cord-104239-xxlcdbqi 95 11 method method NN cord-104239-xxlcdbqi 95 12 , , , cord-104239-xxlcdbqi 95 13 NRK NRK NNP cord-104239-xxlcdbqi 95 14 and and CC cord-104239-xxlcdbqi 95 15 RBL rbl NN cord-104239-xxlcdbqi 95 16 cells cell NNS cord-104239-xxlcdbqi 95 17 were be VBD cord-104239-xxlcdbqi 95 18 grown grow VBN cord-104239-xxlcdbqi 95 19 on on IN cord-104239-xxlcdbqi 95 20 35-mm 35-mm CD cord-104239-xxlcdbqi 95 21 tissue tissue NN cord-104239-xxlcdbqi 95 22 - - HYPH cord-104239-xxlcdbqi 95 23 culture culture NN cord-104239-xxlcdbqi 95 24 dishes dish NNS cord-104239-xxlcdbqi 95 25 ( ( -LRB- cord-104239-xxlcdbqi 95 26 Costar Costar NNP cord-104239-xxlcdbqi 95 27 Corp. Corp. NNP cord-104239-xxlcdbqi 95 28 , , , cord-104239-xxlcdbqi 95 29 Cambridge Cambridge NNP cord-104239-xxlcdbqi 95 30 , , , cord-104239-xxlcdbqi 95 31 MA MA NNP cord-104239-xxlcdbqi 95 32 ) ) -RRB- cord-104239-xxlcdbqi 95 33 , , , cord-104239-xxlcdbqi 95 34 fixed fix VBN cord-104239-xxlcdbqi 95 35 in in IN cord-104239-xxlcdbqi 95 36 the the DT cord-104239-xxlcdbqi 95 37 dish dish NN cord-104239-xxlcdbqi 95 38 in in IN cord-104239-xxlcdbqi 95 39 2.5 2.5 CD cord-104239-xxlcdbqi 95 40 % % NN cord-104239-xxlcdbqi 95 41 GA GA NNP cord-104239-xxlcdbqi 95 42 in in IN cord-104239-xxlcdbqi 95 43 PBS PBS NNP cord-104239-xxlcdbqi 95 44 for for IN cord-104239-xxlcdbqi 95 45 1 1 CD cord-104239-xxlcdbqi 95 46 h h NN cord-104239-xxlcdbqi 95 47 at at IN cord-104239-xxlcdbqi 95 48 rt rt NNP cord-104239-xxlcdbqi 95 49 , , , cord-104239-xxlcdbqi 95 50 and and CC cord-104239-xxlcdbqi 95 51 processed process VBN cord-104239-xxlcdbqi 95 52 for for IN cord-104239-xxlcdbqi 95 53 TEM TEM NNP cord-104239-xxlcdbqi 95 54 as as IN cord-104239-xxlcdbqi 95 55 described describe VBN cord-104239-xxlcdbqi 95 56 above above RB cord-104239-xxlcdbqi 95 57 . . . cord-104239-xxlcdbqi 96 1 The the DT cord-104239-xxlcdbqi 96 2 cell cell NN cord-104239-xxlcdbqi 96 3 pellet pellet NN cord-104239-xxlcdbqi 96 4 was be VBD cord-104239-xxlcdbqi 96 5 cut cut VBN cord-104239-xxlcdbqi 96 6 with with IN cord-104239-xxlcdbqi 96 7 a a DT cord-104239-xxlcdbqi 96 8 diamond diamond NN cord-104239-xxlcdbqi 96 9 knife knife NN cord-104239-xxlcdbqi 96 10 on on IN cord-104239-xxlcdbqi 96 11 a a DT cord-104239-xxlcdbqi 96 12 Reichert Reichert NNP cord-104239-xxlcdbqi 96 13 ultramicrotome ultramicrotome VBD cord-104239-xxlcdbqi 96 14 2E. 2e. CD cord-104239-xxlcdbqi 96 15 60 60 CD cord-104239-xxlcdbqi 96 16 - - HYPH cord-104239-xxlcdbqi 96 17 65-nm 65-nm CD cord-104239-xxlcdbqi 96 18 sections section NNS cord-104239-xxlcdbqi 96 19 were be VBD cord-104239-xxlcdbqi 96 20 counterstained counterstaine VBN cord-104239-xxlcdbqi 96 21 as as IN cord-104239-xxlcdbqi 96 22 described describe VBN cord-104239-xxlcdbqi 96 23 above above RB cord-104239-xxlcdbqi 96 24 . . . cord-104239-xxlcdbqi 97 1 Approximately approximately RB cord-104239-xxlcdbqi 97 2 1,200 1,200 CD cord-104239-xxlcdbqi 97 3 RBL RBL NNP cord-104239-xxlcdbqi 97 4 and and CC cord-104239-xxlcdbqi 97 5 800 800 CD cord-104239-xxlcdbqi 97 6 NRK NRK NNP cord-104239-xxlcdbqi 97 7 cells cell NNS cord-104239-xxlcdbqi 97 8 were be VBD cord-104239-xxlcdbqi 97 9 randomly randomly RB cord-104239-xxlcdbqi 97 10 chosen choose VBN cord-104239-xxlcdbqi 97 11 and and CC cord-104239-xxlcdbqi 97 12 photoenlarged photoenlarge VBN cord-104239-xxlcdbqi 97 13 to to IN cord-104239-xxlcdbqi 97 14 a a DT cord-104239-xxlcdbqi 97 15 magnification magnification NN cord-104239-xxlcdbqi 97 16 of of IN cord-104239-xxlcdbqi 97 17 14,000 14,000 CD cord-104239-xxlcdbqi 97 18 . . . cord-104239-xxlcdbqi 98 1 The the DT cord-104239-xxlcdbqi 98 2 mean mean JJ cord-104239-xxlcdbqi 98 3 surface surface NN cord-104239-xxlcdbqi 98 4 of of IN cord-104239-xxlcdbqi 98 5 the the DT cord-104239-xxlcdbqi 98 6 cells cell NNS cord-104239-xxlcdbqi 98 7 on on IN cord-104239-xxlcdbqi 98 8 sections section NNS cord-104239-xxlcdbqi 98 9 were be VBD cord-104239-xxlcdbqi 98 10 measured measure VBN cord-104239-xxlcdbqi 98 11 by by IN cord-104239-xxlcdbqi 98 12 the the DT cord-104239-xxlcdbqi 98 13 point point NN cord-104239-xxlcdbqi 98 14 - - HYPH cord-104239-xxlcdbqi 98 15 counting counting NN cord-104239-xxlcdbqi 98 16 method method NN cord-104239-xxlcdbqi 98 17 ( ( -LRB- cord-104239-xxlcdbqi 98 18 Weibel Weibel NNP cord-104239-xxlcdbqi 98 19 , , , cord-104239-xxlcdbqi 98 20 1979 1979 CD cord-104239-xxlcdbqi 98 21 ) ) -RRB- cord-104239-xxlcdbqi 98 22 . . . cord-104239-xxlcdbqi 99 1 The the DT cord-104239-xxlcdbqi 99 2 mean mean JJ cord-104239-xxlcdbqi 99 3 cell cell NN cord-104239-xxlcdbqi 99 4 volume volume NN cord-104239-xxlcdbqi 99 5 was be VBD cord-104239-xxlcdbqi 99 6 found find VBN cord-104239-xxlcdbqi 99 7 according accord VBG cord-104239-xxlcdbqi 99 8 to to IN cord-104239-xxlcdbqi 99 9 formula formula NN cord-104239-xxlcdbqi 99 10 ( ( -LRB- cord-104239-xxlcdbqi 99 11 Weibel Weibel NNP cord-104239-xxlcdbqi 99 12 and and CC cord-104239-xxlcdbqi 99 13 Gomez Gomez NNP cord-104239-xxlcdbqi 99 14 , , , cord-104239-xxlcdbqi 99 15 1962 1962 CD cord-104239-xxlcdbqi 99 16 ) ) -RRB- cord-104239-xxlcdbqi 99 17 : : : cord-104239-xxlcdbqi 99 18 where where WRB cord-104239-xxlcdbqi 99 19 Sc.s Sc.s NNP cord-104239-xxlcdbqi 99 20 . . . cord-104239-xxlcdbqi 99 21 is be VBZ cord-104239-xxlcdbqi 99 22 the the DT cord-104239-xxlcdbqi 99 23 mean mean JJ cord-104239-xxlcdbqi 99 24 cell cell NN cord-104239-xxlcdbqi 99 25 surface surface NN cord-104239-xxlcdbqi 99 26 on on IN cord-104239-xxlcdbqi 99 27 thin thin JJ cord-104239-xxlcdbqi 99 28 - - HYPH cord-104239-xxlcdbqi 99 29 section section NN cord-104239-xxlcdbqi 99 30 , , , cord-104239-xxlcdbqi 99 31 The the DT cord-104239-xxlcdbqi 99 32 surface surface NN cord-104239-xxlcdbqi 99 33 to to IN cord-104239-xxlcdbqi 99 34 volume volume NN cord-104239-xxlcdbqi 99 35 ratio ratio NN cord-104239-xxlcdbqi 99 36 was be VBD cord-104239-xxlcdbqi 99 37 found find VBN cord-104239-xxlcdbqi 99 38 according accord VBG cord-104239-xxlcdbqi 99 39 to to IN cord-104239-xxlcdbqi 99 40 formula formula NN cord-104239-xxlcdbqi 100 1 : : : cord-104239-xxlcdbqi 100 2 where where WRB cord-104239-xxlcdbqi 100 3 I -PRON- PRP cord-104239-xxlcdbqi 100 4 is be VBZ cord-104239-xxlcdbqi 100 5 the the DT cord-104239-xxlcdbqi 100 6 number number NN cord-104239-xxlcdbqi 100 7 of of IN cord-104239-xxlcdbqi 100 8 intersections intersection NNS cord-104239-xxlcdbqi 100 9 on on IN cord-104239-xxlcdbqi 100 10 a a DT cord-104239-xxlcdbqi 100 11 grid grid NN cord-104239-xxlcdbqi 100 12 , , , cord-104239-xxlcdbqi 100 13 P p NN cord-104239-xxlcdbqi 100 14 is be VBZ cord-104239-xxlcdbqi 100 15 the the DT cord-104239-xxlcdbqi 100 16 number number NN cord-104239-xxlcdbqi 100 17 of of IN cord-104239-xxlcdbqi 100 18 points point NNS cord-104239-xxlcdbqi 100 19 on on IN cord-104239-xxlcdbqi 100 20 the the DT cord-104239-xxlcdbqi 100 21 grid grid NN cord-104239-xxlcdbqi 100 22 , , , cord-104239-xxlcdbqi 100 23 and and CC cord-104239-xxlcdbqi 100 24 d d NN cord-104239-xxlcdbqi 100 25 is be VBZ cord-104239-xxlcdbqi 100 26 the the DT cord-104239-xxlcdbqi 100 27 distance distance NN cord-104239-xxlcdbqi 100 28 between between IN cord-104239-xxlcdbqi 100 29 the the DT cord-104239-xxlcdbqi 100 30 points point NNS cord-104239-xxlcdbqi 100 31 ( ( -LRB- cord-104239-xxlcdbqi 100 32 Weibel Weibel NNP cord-104239-xxlcdbqi 100 33 , , , cord-104239-xxlcdbqi 100 34 1979 1979 CD cord-104239-xxlcdbqi 100 35 ) ) -RRB- cord-104239-xxlcdbqi 100 36 . . . cord-104239-xxlcdbqi 101 1 Both both DT cord-104239-xxlcdbqi 101 2 procedures procedure NNS cord-104239-xxlcdbqi 101 3 yielded yield VBD cord-104239-xxlcdbqi 101 4 nearly nearly RB cord-104239-xxlcdbqi 101 5 identical identical JJ cord-104239-xxlcdbqi 101 6 results result NNS cord-104239-xxlcdbqi 101 7 for for IN cord-104239-xxlcdbqi 101 8 RBL rbl NN cord-104239-xxlcdbqi 101 9 cells cell NNS cord-104239-xxlcdbqi 101 10 , , , cord-104239-xxlcdbqi 101 11 The the DT cord-104239-xxlcdbqi 101 12 values value NNS cord-104239-xxlcdbqi 101 13 obtained obtain VBN cord-104239-xxlcdbqi 101 14 from from IN cord-104239-xxlcdbqi 101 15 the the DT cord-104239-xxlcdbqi 101 16 second second JJ cord-104239-xxlcdbqi 101 17 procedure procedure NN cord-104239-xxlcdbqi 101 18 are be VBP cord-104239-xxlcdbqi 101 19 reported report VBN cord-104239-xxlcdbqi 101 20 in in IN cord-104239-xxlcdbqi 101 21 Table Table NNP cord-104239-xxlcdbqi 101 22 I -PRON- PRP cord-104239-xxlcdbqi 101 23 . . . cord-104239-xxlcdbqi 102 1 The the DT cord-104239-xxlcdbqi 102 2 stereological stereological JJ cord-104239-xxlcdbqi 102 3 parameters parameter NNS cord-104239-xxlcdbqi 102 4 of of IN cord-104239-xxlcdbqi 102 5 nuclei nucleus NNS cord-104239-xxlcdbqi 102 6 , , , cord-104239-xxlcdbqi 102 7 ER ER NNP cord-104239-xxlcdbqi 102 8 , , , cord-104239-xxlcdbqi 102 9 and and CC cord-104239-xxlcdbqi 102 10 Golgi Golgi NNP cord-104239-xxlcdbqi 102 11 apparatus apparatus NN cord-104239-xxlcdbqi 102 12 were be VBD cord-104239-xxlcdbqi 102 13 determined determine VBN cord-104239-xxlcdbqi 102 14 using use VBG cord-104239-xxlcdbqi 102 15 the the DT cord-104239-xxlcdbqi 102 16 same same JJ cord-104239-xxlcdbqi 102 17 sections section NNS cord-104239-xxlcdbqi 102 18 . . . cord-104239-xxlcdbqi 103 1 Randomly randomly RB cord-104239-xxlcdbqi 103 2 taken take VBN cord-104239-xxlcdbqi 103 3 cell cell NN cord-104239-xxlcdbqi 103 4 contours contour NNS cord-104239-xxlcdbqi 103 5 were be VBD cord-104239-xxlcdbqi 103 6 enlarged enlarge VBN cord-104239-xxlcdbqi 103 7 to to IN cord-104239-xxlcdbqi 103 8 a a DT cord-104239-xxlcdbqi 103 9 total total JJ cord-104239-xxlcdbqi 103 10 magnification magnification NN cord-104239-xxlcdbqi 103 11 of of IN cord-104239-xxlcdbqi 103 12 14,000 14,000 CD cord-104239-xxlcdbqi 103 13 as as IN cord-104239-xxlcdbqi 103 14 described describe VBN cord-104239-xxlcdbqi 103 15 above above RB cord-104239-xxlcdbqi 103 16 . . . cord-104239-xxlcdbqi 104 1 Using use VBG cord-104239-xxlcdbqi 104 2 the the DT cord-104239-xxlcdbqi 104 3 point point NN cord-104239-xxlcdbqi 104 4 - - HYPH cord-104239-xxlcdbqi 104 5 counting counting NN cord-104239-xxlcdbqi 104 6 method method NN cord-104239-xxlcdbqi 104 7 , , , cord-104239-xxlcdbqi 104 8 we -PRON- PRP cord-104239-xxlcdbqi 104 9 determined determine VBD cord-104239-xxlcdbqi 104 10 the the DT cord-104239-xxlcdbqi 104 11 nucleus nucleus JJ cord-104239-xxlcdbqi 104 12 volume volume NN cord-104239-xxlcdbqi 104 13 to to IN cord-104239-xxlcdbqi 104 14 cell cell NN cord-104239-xxlcdbqi 104 15 volume volume NN cord-104239-xxlcdbqi 104 16 ratio ratio NN cord-104239-xxlcdbqi 104 17 and and CC cord-104239-xxlcdbqi 104 18 a a DT cord-104239-xxlcdbqi 104 19 nucleus nucleus NN cord-104239-xxlcdbqi 104 20 surface surface NN cord-104239-xxlcdbqi 104 21 to to IN cord-104239-xxlcdbqi 104 22 nucleus nucleus NN cord-104239-xxlcdbqi 104 23 volume volume NN cord-104239-xxlcdbqi 104 24 ratio ratio NN cord-104239-xxlcdbqi 104 25 as as IN cord-104239-xxlcdbqi 104 26 described describe VBN cord-104239-xxlcdbqi 104 27 ( ( -LRB- cord-104239-xxlcdbqi 104 28 Weibel Weibel NNP cord-104239-xxlcdbqi 104 29 , , , cord-104239-xxlcdbqi 104 30 1979 1979 CD cord-104239-xxlcdbqi 104 31 ) ) -RRB- cord-104239-xxlcdbqi 104 32 . . . cord-104239-xxlcdbqi 105 1 Randomly randomly RB cord-104239-xxlcdbqi 105 2 chosen choose VBN cord-104239-xxlcdbqi 105 3 fields field NNS cord-104239-xxlcdbqi 105 4 of of IN cord-104239-xxlcdbqi 105 5 cells cell NNS cord-104239-xxlcdbqi 105 6 containing contain VBG cord-104239-xxlcdbqi 105 7 ER ER NNP cord-104239-xxlcdbqi 105 8 or or CC cord-104239-xxlcdbqi 105 9 Golgi Golgi NNP cord-104239-xxlcdbqi 105 10 membranes membrane NNS cord-104239-xxlcdbqi 105 11 were be VBD cord-104239-xxlcdbqi 105 12 magnified magnify VBN cord-104239-xxlcdbqi 105 13 to to IN cord-104239-xxlcdbqi 105 14 31,000 31,000 CD cord-104239-xxlcdbqi 105 15 to to TO cord-104239-xxlcdbqi 105 16 establish establish VB cord-104239-xxlcdbqi 105 17 the the DT cord-104239-xxlcdbqi 105 18 ER(Golgi ER(Golgi NNP cord-104239-xxlcdbqi 105 19 ) ) -RRB- cord-104239-xxlcdbqi 105 20 volume volume NN cord-104239-xxlcdbqi 105 21 to to IN cord-104239-xxlcdbqi 105 22 cell cell NN cord-104239-xxlcdbqi 105 23 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 105 24 volume volume NN cord-104239-xxlcdbqi 105 25 ratio ratio NN cord-104239-xxlcdbqi 105 26 by by IN cord-104239-xxlcdbqi 105 27 the the DT cord-104239-xxlcdbqi 105 28 point point NN cord-104239-xxlcdbqi 105 29 - - HYPH cord-104239-xxlcdbqi 105 30 counting counting NN cord-104239-xxlcdbqi 105 31 method method NN cord-104239-xxlcdbqi 105 32 ( ( -LRB- cord-104239-xxlcdbqi 105 33 Weibel Weibel NNP cord-104239-xxlcdbqi 105 34 , , , cord-104239-xxlcdbqi 105 35 1979 1979 CD cord-104239-xxlcdbqi 105 36 ) ) -RRB- cord-104239-xxlcdbqi 105 37 . . . cord-104239-xxlcdbqi 106 1 Some some DT cord-104239-xxlcdbqi 106 2 of of IN cord-104239-xxlcdbqi 106 3 these these DT cord-104239-xxlcdbqi 106 4 images image NNS cord-104239-xxlcdbqi 106 5 were be VBD cord-104239-xxlcdbqi 106 6 photoenlarged photoenlarge VBN cord-104239-xxlcdbqi 106 7 to to IN cord-104239-xxlcdbqi 106 8 120,000 120,000 CD cord-104239-xxlcdbqi 106 9 to to TO cord-104239-xxlcdbqi 106 10 determine determine VB cord-104239-xxlcdbqi 106 11 the the DT cord-104239-xxlcdbqi 106 12 ER(Golgi ER(Golgi NNP cord-104239-xxlcdbqi 106 13 ) ) -RRB- cord-104239-xxlcdbqi 106 14 surface surface NN cord-104239-xxlcdbqi 106 15 to to IN cord-104239-xxlcdbqi 106 16 volume volume NN cord-104239-xxlcdbqi 106 17 ratio ratio NN cord-104239-xxlcdbqi 106 18 . . . cord-104239-xxlcdbqi 107 1 The the DT cord-104239-xxlcdbqi 107 2 ER(Golgi ER(Golgi NNP cord-104239-xxlcdbqi 107 3 ) ) -RRB- cord-104239-xxlcdbqi 107 4 surface surface NN cord-104239-xxlcdbqi 107 5 was be VBD cord-104239-xxlcdbqi 107 6 found find VBN cord-104239-xxlcdbqi 107 7 by by IN cord-104239-xxlcdbqi 107 8 formula formula NN cord-104239-xxlcdbqi 107 9 3 3 CD cord-104239-xxlcdbqi 107 10 . . . cord-104239-xxlcdbqi 108 1 Corrections correction NNS cord-104239-xxlcdbqi 108 2 of of IN cord-104239-xxlcdbqi 108 3 bias bias NN cord-104239-xxlcdbqi 108 4 due due IN cord-104239-xxlcdbqi 108 5 to to IN cord-104239-xxlcdbqi 108 6 section section NN cord-104239-xxlcdbqi 108 7 thickness thickness NN cord-104239-xxlcdbqi 108 8 were be VBD cord-104239-xxlcdbqi 108 9 done do VBN cord-104239-xxlcdbqi 108 10 as as IN cord-104239-xxlcdbqi 108 11 described describe VBN cord-104239-xxlcdbqi 108 12 ( ( -LRB- cord-104239-xxlcdbqi 108 13 Weibel Weibel NNP cord-104239-xxlcdbqi 108 14 and and CC cord-104239-xxlcdbqi 108 15 Paumgartner Paumgartner NNP cord-104239-xxlcdbqi 108 16 , , , cord-104239-xxlcdbqi 108 17 1978 1978 CD cord-104239-xxlcdbqi 108 18 ) ) -RRB- cord-104239-xxlcdbqi 108 19 . . . cord-104239-xxlcdbqi 109 1 For for IN cord-104239-xxlcdbqi 109 2 the the DT cord-104239-xxlcdbqi 109 3 ER ER NNP cord-104239-xxlcdbqi 109 4 volume volume NN cord-104239-xxlcdbqi 109 5 , , , cord-104239-xxlcdbqi 109 6 the the DT cord-104239-xxlcdbqi 109 7 correction correction NN cord-104239-xxlcdbqi 109 8 factor factor NN cord-104239-xxlcdbqi 109 9 was be VBD cord-104239-xxlcdbqi 109 10 0.51 0.51 CD cord-104239-xxlcdbqi 109 11 and and CC cord-104239-xxlcdbqi 109 12 0.65 0.65 CD cord-104239-xxlcdbqi 109 13 for for IN cord-104239-xxlcdbqi 109 14 the the DT cord-104239-xxlcdbqi 109 15 ER ER NNP cord-104239-xxlcdbqi 109 16 surface surface NN cord-104239-xxlcdbqi 109 17 . . . cord-104239-xxlcdbqi 110 1 Assuming assume VBG cord-104239-xxlcdbqi 110 2 that that IN cord-104239-xxlcdbqi 110 3 two two CD cord-104239-xxlcdbqi 110 4 - - HYPH cord-104239-xxlcdbqi 110 5 thirds third NNS cord-104239-xxlcdbqi 110 6 of of IN cord-104239-xxlcdbqi 110 7 the the DT cord-104239-xxlcdbqi 110 8 Golgi Golgi NNP cord-104239-xxlcdbqi 110 9 complex complex NN cord-104239-xxlcdbqi 110 10 was be VBD cord-104239-xxlcdbqi 110 11 composed compose VBN cord-104239-xxlcdbqi 110 12 of of IN cord-104239-xxlcdbqi 110 13 cisternae cisternae NN cord-104239-xxlcdbqi 110 14 and and CC cord-104239-xxlcdbqi 110 15 one one CD cord-104239-xxlcdbqi 110 16 - - HYPH cord-104239-xxlcdbqi 110 17 third third NN cord-104239-xxlcdbqi 110 18 of of IN cord-104239-xxlcdbqi 110 19 tubules tubule NNS cord-104239-xxlcdbqi 110 20 , , , cord-104239-xxlcdbqi 110 21 the the DT cord-104239-xxlcdbqi 110 22 correction correction NN cord-104239-xxlcdbqi 110 23 factors factor NNS cord-104239-xxlcdbqi 110 24 for for IN cord-104239-xxlcdbqi 110 25 Golgi Golgi NNP cord-104239-xxlcdbqi 110 26 volume volume NN cord-104239-xxlcdbqi 110 27 and and CC cord-104239-xxlcdbqi 110 28 surface surface NN cord-104239-xxlcdbqi 110 29 were be VBD cord-104239-xxlcdbqi 110 30 estimated estimate VBN cord-104239-xxlcdbqi 110 31 to to TO cord-104239-xxlcdbqi 110 32 be be VB cord-104239-xxlcdbqi 110 33 0.6 0.6 CD cord-104239-xxlcdbqi 110 34 and and CC cord-104239-xxlcdbqi 110 35 0.64 0.64 CD cord-104239-xxlcdbqi 110 36 , , , cord-104239-xxlcdbqi 110 37 respectively respectively RB cord-104239-xxlcdbqi 110 38 . . . cord-104239-xxlcdbqi 111 1 Estimation estimation NN cord-104239-xxlcdbqi 111 2 of of IN cord-104239-xxlcdbqi 111 3 Number Number NNP cord-104239-xxlcdbqi 111 4 and and CC cord-104239-xxlcdbqi 111 5 Deasity Deasity NNP cord-104239-xxlcdbqi 111 6 of of IN cord-104239-xxlcdbqi 111 7 Total total JJ cord-104239-xxlcdbqi 111 8 ER er NN cord-104239-xxlcdbqi 111 9 - - HYPH cord-104239-xxlcdbqi 111 10 derived derive VBN cord-104239-xxlcdbqi 111 11 Buds bud NNS cord-104239-xxlcdbqi 111 12 . . . cord-104239-xxlcdbqi 112 1 To to TO cord-104239-xxlcdbqi 112 2 estimate estimate VB cord-104239-xxlcdbqi 112 3 the the DT cord-104239-xxlcdbqi 112 4 number number NN cord-104239-xxlcdbqi 112 5 ER er NN cord-104239-xxlcdbqi 112 6 - - HYPH cord-104239-xxlcdbqi 112 7 derived derive VBN cord-104239-xxlcdbqi 112 8 buds bud NNS cord-104239-xxlcdbqi 112 9 , , , cord-104239-xxlcdbqi 112 10 we -PRON- PRP cord-104239-xxlcdbqi 112 11 used use VBD cord-104239-xxlcdbqi 112 12 two two CD cord-104239-xxlcdbqi 112 13 distinct distinct JJ cord-104239-xxlcdbqi 112 14 criteria criterion NNS cord-104239-xxlcdbqi 112 15 : : : cord-104239-xxlcdbqi 112 16 ( ( -LRB- cord-104239-xxlcdbqi 112 17 a a DT cord-104239-xxlcdbqi 113 1 ) ) -RRB- cord-104239-xxlcdbqi 113 2 a a DT cord-104239-xxlcdbqi 113 3 bud bud NN cord-104239-xxlcdbqi 113 4 was be VBD cord-104239-xxlcdbqi 113 5 considered consider VBN cord-104239-xxlcdbqi 113 6 an an DT cord-104239-xxlcdbqi 113 7 elevation elevation NN cord-104239-xxlcdbqi 113 8 on on IN cord-104239-xxlcdbqi 113 9 the the DT cord-104239-xxlcdbqi 113 10 surface surface NN cord-104239-xxlcdbqi 113 11 of of IN cord-104239-xxlcdbqi 113 12 the the DT cord-104239-xxlcdbqi 113 13 ER ER NNP cord-104239-xxlcdbqi 113 14 with with IN cord-104239-xxlcdbqi 113 15 a a DT cord-104239-xxlcdbqi 113 16 width width NN cord-104239-xxlcdbqi 113 17 of of IN cord-104239-xxlcdbqi 113 18 60 60 CD cord-104239-xxlcdbqi 113 19 - - SYM cord-104239-xxlcdbqi 113 20 80 80 CD cord-104239-xxlcdbqi 113 21 nm nm NN cord-104239-xxlcdbqi 113 22 and and CC cord-104239-xxlcdbqi 113 23 covered cover VBN cord-104239-xxlcdbqi 113 24 with with IN cord-104239-xxlcdbqi 113 25 a a DT cord-104239-xxlcdbqi 113 26 characteristic characteristic JJ cord-104239-xxlcdbqi 113 27 coat coat NN cord-104239-xxlcdbqi 113 28 on on IN cord-104239-xxlcdbqi 113 29 the the DT cord-104239-xxlcdbqi 113 30 external external JJ cord-104239-xxlcdbqi 113 31 leaflet leaflet NN cord-104239-xxlcdbqi 113 32 of of IN cord-104239-xxlcdbqi 113 33 the the DT cord-104239-xxlcdbqi 113 34 membrane membrane NN cord-104239-xxlcdbqi 113 35 : : : cord-104239-xxlcdbqi 113 36 and and CC cord-104239-xxlcdbqi 113 37 ( ( -LRB- cord-104239-xxlcdbqi 113 38 b b NN cord-104239-xxlcdbqi 113 39 ) ) -RRB- cord-104239-xxlcdbqi 113 40 buds bud NNS cord-104239-xxlcdbqi 113 41 had have VBD cord-104239-xxlcdbqi 113 42 a a DT cord-104239-xxlcdbqi 113 43 direct direct JJ cord-104239-xxlcdbqi 113 44 connection connection NN cord-104239-xxlcdbqi 113 45 with with IN cord-104239-xxlcdbqi 113 46 the the DT cord-104239-xxlcdbqi 113 47 ER ER NNP cord-104239-xxlcdbqi 113 48 membrane membrane NN cord-104239-xxlcdbqi 113 49 and and CC cord-104239-xxlcdbqi 113 50 were be VBD cord-104239-xxlcdbqi 113 51 extruded extrude VBN cord-104239-xxlcdbqi 113 52 from from IN cord-104239-xxlcdbqi 113 53 the the DT cord-104239-xxlcdbqi 113 54 membrane membrane NN cord-104239-xxlcdbqi 113 55 by by IN cord-104239-xxlcdbqi 113 56 at at RB cord-104239-xxlcdbqi 113 57 least least RBS cord-104239-xxlcdbqi 113 58 50 50 CD cord-104239-xxlcdbqi 113 59 % % NN cord-104239-xxlcdbqi 113 60 of of IN cord-104239-xxlcdbqi 113 61 their -PRON- PRP$ cord-104239-xxlcdbqi 113 62 circumference circumference NN cord-104239-xxlcdbqi 113 63 . . . cord-104239-xxlcdbqi 114 1 The the DT cord-104239-xxlcdbqi 114 2 total total JJ cord-104239-xxlcdbqi 114 3 number number NN cord-104239-xxlcdbqi 114 4 of of IN cord-104239-xxlcdbqi 114 5 ER ER NNP cord-104239-xxlcdbqi 114 6 buds bud NNS cord-104239-xxlcdbqi 114 7 per per IN cord-104239-xxlcdbqi 114 8 cell cell NN cord-104239-xxlcdbqi 114 9 was be VBD cord-104239-xxlcdbqi 114 10 determined determine VBN cord-104239-xxlcdbqi 114 11 as as IN cord-104239-xxlcdbqi 114 12 described describe VBN cord-104239-xxlcdbqi 114 13 ( ( -LRB- cord-104239-xxlcdbqi 114 14 Lucocq Lucocq NNP cord-104239-xxlcdbqi 114 15 et et FW cord-104239-xxlcdbqi 114 16 al al NNP cord-104239-xxlcdbqi 114 17 . . NNP cord-104239-xxlcdbqi 114 18 , , , cord-104239-xxlcdbqi 114 19 1989 1989 CD cord-104239-xxlcdbqi 114 20 ) ) -RRB- cord-104239-xxlcdbqi 114 21 . . . cord-104239-xxlcdbqi 115 1 To to TO cord-104239-xxlcdbqi 115 2 obtain obtain VB cord-104239-xxlcdbqi 115 3 this this DT cord-104239-xxlcdbqi 115 4 value value NN cord-104239-xxlcdbqi 115 5 , , , cord-104239-xxlcdbqi 115 6 we -PRON- PRP cord-104239-xxlcdbqi 115 7 multiplied multiply VBD cord-104239-xxlcdbqi 115 8 the the DT cord-104239-xxlcdbqi 115 9 total total JJ cord-104239-xxlcdbqi 115 10 number number NN cord-104239-xxlcdbqi 115 11 of of IN cord-104239-xxlcdbqi 115 12 ER ER NNP cord-104239-xxlcdbqi 115 13 buds bud NNS cord-104239-xxlcdbqi 115 14 detected detect VBN cord-104239-xxlcdbqi 115 15 in in IN cord-104239-xxlcdbqi 115 16 thin thin JJ cord-104239-xxlcdbqi 115 17 - - HYPH cord-104239-xxlcdbqi 115 18 secfions secfion NNS cord-104239-xxlcdbqi 115 19 of of IN cord-104239-xxlcdbqi 115 20 ~300 ~300 JJ cord-104239-xxlcdbqi 115 21 cells cell NNS cord-104239-xxlcdbqi 115 22 by by IN cord-104239-xxlcdbqi 115 23 the the DT cord-104239-xxlcdbqi 115 24 mean mean JJ cord-104239-xxlcdbqi 115 25 volume volume NN cord-104239-xxlcdbqi 115 26 of of IN cord-104239-xxlcdbqi 115 27 cells cell NNS cord-104239-xxlcdbqi 115 28 and and CC cord-104239-xxlcdbqi 115 29 divided divide VBD cord-104239-xxlcdbqi 115 30 that that DT cord-104239-xxlcdbqi 115 31 value value NN cord-104239-xxlcdbqi 115 32 by by IN cord-104239-xxlcdbqi 115 33 the the DT cord-104239-xxlcdbqi 115 34 total total JJ cord-104239-xxlcdbqi 115 35 volume volume NN cord-104239-xxlcdbqi 115 36 contributed contribute VBN cord-104239-xxlcdbqi 115 37 by by IN cord-104239-xxlcdbqi 115 38 the the DT cord-104239-xxlcdbqi 115 39 ~300 ~300 JJ cord-104239-xxlcdbqi 115 40 cells cell NNS cord-104239-xxlcdbqi 115 41 . . . cord-104239-xxlcdbqi 116 1 The the DT cord-104239-xxlcdbqi 116 2 latter latter JJ cord-104239-xxlcdbqi 116 3 value value NN cord-104239-xxlcdbqi 116 4 was be VBD cord-104239-xxlcdbqi 116 5 found find VBN cord-104239-xxlcdbqi 116 6 by by IN cord-104239-xxlcdbqi 116 7 multiplying multiply VBG cord-104239-xxlcdbqi 116 8 the the DT cord-104239-xxlcdbqi 116 9 thickness thickness NN cord-104239-xxlcdbqi 116 10 of of IN cord-104239-xxlcdbqi 116 11 a a DT cord-104239-xxlcdbqi 116 12 section section NN cord-104239-xxlcdbqi 116 13 by by IN cord-104239-xxlcdbqi 116 14 the the DT cord-104239-xxlcdbqi 116 15 total total JJ cord-104239-xxlcdbqi 116 16 surface surface NN cord-104239-xxlcdbqi 116 17 area area NN cord-104239-xxlcdbqi 116 18 of of IN cord-104239-xxlcdbqi 116 19 the the DT cord-104239-xxlcdbqi 116 20 ~300 ~300 JJ cord-104239-xxlcdbqi 116 21 cell cell NN cord-104239-xxlcdbqi 116 22 sections section NNS cord-104239-xxlcdbqi 116 23 in in IN cord-104239-xxlcdbqi 116 24 which which WDT cord-104239-xxlcdbqi 116 25 ER ER NNP cord-104239-xxlcdbqi 116 26 buds bud NNS cord-104239-xxlcdbqi 116 27 were be VBD cord-104239-xxlcdbqi 116 28 counted count VBN cord-104239-xxlcdbqi 116 29 . . . cord-104239-xxlcdbqi 117 1 By by IN cord-104239-xxlcdbqi 117 2 using use VBG cord-104239-xxlcdbqi 117 3 relatively relatively RB cord-104239-xxlcdbqi 117 4 thick thick JJ cord-104239-xxlcdbqi 117 5 sections section NNS cord-104239-xxlcdbqi 117 6 corresponding correspond VBG cord-104239-xxlcdbqi 117 7 to to IN cord-104239-xxlcdbqi 117 8 the the DT cord-104239-xxlcdbqi 117 9 average average JJ cord-104239-xxlcdbqi 117 10 diameter diameter NN cord-104239-xxlcdbqi 117 11 of of IN cord-104239-xxlcdbqi 117 12 an an DT cord-104239-xxlcdbqi 117 13 ER ER NNP cord-104239-xxlcdbqi 117 14 bud bud NN cord-104239-xxlcdbqi 117 15 and and CC cord-104239-xxlcdbqi 117 16 the the DT cord-104239-xxlcdbqi 117 17 above above JJ cord-104239-xxlcdbqi 117 18 two two CD cord-104239-xxlcdbqi 117 19 criteria criterion NNS cord-104239-xxlcdbqi 117 20 , , , cord-104239-xxlcdbqi 117 21 we -PRON- PRP cord-104239-xxlcdbqi 117 22 were be VBD cord-104239-xxlcdbqi 117 23 able able JJ cord-104239-xxlcdbqi 117 24 to to TO cord-104239-xxlcdbqi 117 25 avoid avoid VB cord-104239-xxlcdbqi 117 26 double double JJ cord-104239-xxlcdbqi 117 27 counting counting NN cord-104239-xxlcdbqi 117 28 most most JJS cord-104239-xxlcdbqi 117 29 of of IN cord-104239-xxlcdbqi 117 30 the the DT cord-104239-xxlcdbqi 117 31 ER ER NNP cord-104239-xxlcdbqi 117 32 buds bud NNS cord-104239-xxlcdbqi 117 33 present present JJ cord-104239-xxlcdbqi 117 34 on on IN cord-104239-xxlcdbqi 117 35 consecutive consecutive JJ cord-104239-xxlcdbqi 117 36 thin thin JJ cord-104239-xxlcdbqi 117 37 sections section NNS cord-104239-xxlcdbqi 117 38 . . . cord-104239-xxlcdbqi 118 1 To to TO cord-104239-xxlcdbqi 118 2 establish establish VB cord-104239-xxlcdbqi 118 3 this this DT cord-104239-xxlcdbqi 118 4 point point NN cord-104239-xxlcdbqi 118 5 independently independently RB cord-104239-xxlcdbqi 118 6 , , , cord-104239-xxlcdbqi 118 7 we -PRON- PRP cord-104239-xxlcdbqi 118 8 performed perform VBD cord-104239-xxlcdbqi 118 9 a a DT cord-104239-xxlcdbqi 118 10 separate separate JJ cord-104239-xxlcdbqi 118 11 experiment experiment NN cord-104239-xxlcdbqi 118 12 in in IN cord-104239-xxlcdbqi 118 13 which which WDT cord-104239-xxlcdbqi 118 14 the the DT cord-104239-xxlcdbqi 118 15 total total JJ cord-104239-xxlcdbqi 118 16 number number NN cord-104239-xxlcdbqi 118 17 of of IN cord-104239-xxlcdbqi 118 18 ER ER NNP cord-104239-xxlcdbqi 118 19 buds bud NNS cord-104239-xxlcdbqi 118 20 was be VBD cord-104239-xxlcdbqi 118 21 counted count VBN cord-104239-xxlcdbqi 118 22 on on IN cord-104239-xxlcdbqi 118 23 several several JJ cord-104239-xxlcdbqi 118 24 stacks stack NNS cord-104239-xxlcdbqi 118 25 of of IN cord-104239-xxlcdbqi 118 26 serial serial JJ cord-104239-xxlcdbqi 118 27 thin thin JJ cord-104239-xxlcdbqi 118 28 - - HYPH cord-104239-xxlcdbqi 118 29 sections section NNS cord-104239-xxlcdbqi 118 30 . . . cord-104239-xxlcdbqi 119 1 By by IN cord-104239-xxlcdbqi 119 2 comparing compare VBG cord-104239-xxlcdbqi 119 3 the the DT cord-104239-xxlcdbqi 119 4 number number NN cord-104239-xxlcdbqi 119 5 of of IN cord-104239-xxlcdbqi 119 6 ER ER NNP cord-104239-xxlcdbqi 119 7 buds bud NNS cord-104239-xxlcdbqi 119 8 determined determine VBN cord-104239-xxlcdbqi 119 9 by by IN cord-104239-xxlcdbqi 119 10 this this DT cord-104239-xxlcdbqi 119 11 reconstruction reconstruction NN cord-104239-xxlcdbqi 119 12 approach approach NN cord-104239-xxlcdbqi 119 13 to to IN cord-104239-xxlcdbqi 119 14 the the DT cord-104239-xxlcdbqi 119 15 number number NN cord-104239-xxlcdbqi 119 16 determined determine VBN cord-104239-xxlcdbqi 119 17 by by IN cord-104239-xxlcdbqi 119 18 counting count VBG cord-104239-xxlcdbqi 119 19 of of IN cord-104239-xxlcdbqi 119 20 individual individual JJ cord-104239-xxlcdbqi 119 21 sections section NNS cord-104239-xxlcdbqi 119 22 ( ( -LRB- cord-104239-xxlcdbqi 119 23 indirect indirect JJ cord-104239-xxlcdbqi 119 24 method method NN cord-104239-xxlcdbqi 119 25 ) ) -RRB- cord-104239-xxlcdbqi 119 26 , , , cord-104239-xxlcdbqi 119 27 we -PRON- PRP cord-104239-xxlcdbqi 119 28 found find VBD cord-104239-xxlcdbqi 119 29 that that IN cord-104239-xxlcdbqi 119 30 overestimation overestimation NN cord-104239-xxlcdbqi 119 31 by by IN cord-104239-xxlcdbqi 119 32 the the DT cord-104239-xxlcdbqi 119 33 latter latter JJ cord-104239-xxlcdbqi 119 34 technique technique NN cord-104239-xxlcdbqi 119 35 was be VBD cord-104239-xxlcdbqi 119 36 < < XX cord-104239-xxlcdbqi 119 37 10 10 CD cord-104239-xxlcdbqi 119 38 - - SYM cord-104239-xxlcdbqi 119 39 15 15 CD cord-104239-xxlcdbqi 119 40 % % NN cord-104239-xxlcdbqi 119 41 . . . cord-104239-xxlcdbqi 120 1 Hence hence RB cord-104239-xxlcdbqi 120 2 , , , cord-104239-xxlcdbqi 120 3 both both DT cord-104239-xxlcdbqi 120 4 methods method NNS cord-104239-xxlcdbqi 120 5 could could MD cord-104239-xxlcdbqi 120 6 be be VB cord-104239-xxlcdbqi 120 7 used use VBN cord-104239-xxlcdbqi 120 8 interchangeably interchangeably RB cord-104239-xxlcdbqi 120 9 . . . cord-104239-xxlcdbqi 121 1 We -PRON- PRP cord-104239-xxlcdbqi 121 2 also also RB cord-104239-xxlcdbqi 121 3 found find VBD cord-104239-xxlcdbqi 121 4 that that IN cord-104239-xxlcdbqi 121 5 the the DT cord-104239-xxlcdbqi 121 6 indirect indirect JJ cord-104239-xxlcdbqi 121 7 method method NN cord-104239-xxlcdbqi 121 8 showed show VBD cord-104239-xxlcdbqi 121 9 a a DT cord-104239-xxlcdbqi 121 10 high high JJ cord-104239-xxlcdbqi 121 11 reproducibility reproducibility NN cord-104239-xxlcdbqi 121 12 . . . cord-104239-xxlcdbqi 122 1 In in IN cord-104239-xxlcdbqi 122 2 spite spite NN cord-104239-xxlcdbqi 122 3 of of IN cord-104239-xxlcdbqi 122 4 the the DT cord-104239-xxlcdbqi 122 5 high high JJ cord-104239-xxlcdbqi 122 6 degree degree NN cord-104239-xxlcdbqi 122 7 of of IN cord-104239-xxlcdbqi 122 8 ER ER NNP cord-104239-xxlcdbqi 122 9 bud bud NN cord-104239-xxlcdbqi 122 10 enrichment enrichment NN cord-104239-xxlcdbqi 122 11 in in IN cord-104239-xxlcdbqi 122 12 local local JJ cord-104239-xxlcdbqi 122 13 zones zone NNS cord-104239-xxlcdbqi 122 14 , , , cord-104239-xxlcdbqi 122 15 the the DT cord-104239-xxlcdbqi 122 16 comparison comparison NN cord-104239-xxlcdbqi 122 17 of of IN cord-104239-xxlcdbqi 122 18 estimates estimate NNS cord-104239-xxlcdbqi 122 19 of of IN cord-104239-xxlcdbqi 122 20 the the DT cord-104239-xxlcdbqi 122 21 total total JJ cord-104239-xxlcdbqi 122 22 number number NN cord-104239-xxlcdbqi 122 23 of of IN cord-104239-xxlcdbqi 122 24 buds bud NNS cord-104239-xxlcdbqi 122 25 per per IN cord-104239-xxlcdbqi 122 26 cells cell NNS cord-104239-xxlcdbqi 122 27 between between IN cord-104239-xxlcdbqi 122 28 groups group NNS cord-104239-xxlcdbqi 122 29 of of IN cord-104239-xxlcdbqi 122 30 as as RB cord-104239-xxlcdbqi 122 31 few few JJ cord-104239-xxlcdbqi 122 32 as as IN cord-104239-xxlcdbqi 122 33 35 35 CD cord-104239-xxlcdbqi 122 34 randomly randomly RB cord-104239-xxlcdbqi 122 35 taken take VBN cord-104239-xxlcdbqi 122 36 cells cell NNS cord-104239-xxlcdbqi 122 37 with with IN cord-104239-xxlcdbqi 122 38 a a DT cord-104239-xxlcdbqi 122 39 total total JJ cord-104239-xxlcdbqi 122 40 surface surface NN cord-104239-xxlcdbqi 122 41 of of IN cord-104239-xxlcdbqi 122 42 section section NN cord-104239-xxlcdbqi 122 43 of of IN cord-104239-xxlcdbqi 122 44 2,500 2,500 CD cord-104239-xxlcdbqi 122 45 tJ.m tj.m CD cord-104239-xxlcdbqi 122 46 2 2 CD cord-104239-xxlcdbqi 122 47 gave give VBD cord-104239-xxlcdbqi 122 48 a a DT cord-104239-xxlcdbqi 122 49 value value NN cord-104239-xxlcdbqi 122 50 of of IN cord-104239-xxlcdbqi 122 51 variance variance NN cord-104239-xxlcdbqi 122 52 of of IN cord-104239-xxlcdbqi 122 53 less less JJR cord-104239-xxlcdbqi 122 54 than than IN cord-104239-xxlcdbqi 122 55 5 5 CD cord-104239-xxlcdbqi 122 56 % % NN cord-104239-xxlcdbqi 122 57 from from IN cord-104239-xxlcdbqi 122 58 one one CD cord-104239-xxlcdbqi 122 59 group group NN cord-104239-xxlcdbqi 122 60 to to IN cord-104239-xxlcdbqi 122 61 another another DT cord-104239-xxlcdbqi 122 62 . . . cord-104239-xxlcdbqi 123 1 In in IN cord-104239-xxlcdbqi 123 2 addition addition NN cord-104239-xxlcdbqi 123 3 , , , cord-104239-xxlcdbqi 123 4 in in IN cord-104239-xxlcdbqi 123 5 four four CD cord-104239-xxlcdbqi 123 6 different different JJ cord-104239-xxlcdbqi 123 7 experiments experiment NNS cord-104239-xxlcdbqi 123 8 , , , cord-104239-xxlcdbqi 123 9 the the DT cord-104239-xxlcdbqi 123 10 value value NN cord-104239-xxlcdbqi 123 11 of of IN cord-104239-xxlcdbqi 123 12 the the DT cord-104239-xxlcdbqi 123 13 total total JJ cord-104239-xxlcdbqi 123 14 number number NN cord-104239-xxlcdbqi 123 15 of of IN cord-104239-xxlcdbqi 123 16 buds bud NNS cord-104239-xxlcdbqi 123 17 per per IN cord-104239-xxlcdbqi 123 18 cell cell NN cord-104239-xxlcdbqi 123 19 was be VBD cord-104239-xxlcdbqi 123 20 found find VBN cord-104239-xxlcdbqi 123 21 to to TO cord-104239-xxlcdbqi 123 22 be be VB cord-104239-xxlcdbqi 123 23 the the DT cord-104239-xxlcdbqi 123 24 same same JJ cord-104239-xxlcdbqi 123 25 . . . cord-104239-xxlcdbqi 124 1 Because because IN cord-104239-xxlcdbqi 124 2 of of IN cord-104239-xxlcdbqi 124 3 technical technical JJ cord-104239-xxlcdbqi 124 4 simplicity simplicity NN cord-104239-xxlcdbqi 124 5 , , , cord-104239-xxlcdbqi 124 6 we -PRON- PRP cord-104239-xxlcdbqi 124 7 routinely routinely RB cord-104239-xxlcdbqi 124 8 used use VBD cord-104239-xxlcdbqi 124 9 the the DT cord-104239-xxlcdbqi 124 10 indirect indirect JJ cord-104239-xxlcdbqi 124 11 method method NN cord-104239-xxlcdbqi 124 12 to to TO cord-104239-xxlcdbqi 124 13 estimate estimate VB cord-104239-xxlcdbqi 124 14 the the DT cord-104239-xxlcdbqi 124 15 average average JJ cord-104239-xxlcdbqi 124 16 number number NN cord-104239-xxlcdbqi 124 17 of of IN cord-104239-xxlcdbqi 124 18 buds bud NNS cord-104239-xxlcdbqi 124 19 per per IN cord-104239-xxlcdbqi 124 20 cell cell NN cord-104239-xxlcdbqi 124 21 . . . cord-104239-xxlcdbqi 125 1 Buds bud NNS cord-104239-xxlcdbqi 125 2 in in IN cord-104239-xxlcdbqi 125 3 the the DT cord-104239-xxlcdbqi 125 4 GolgiExclusion GolgiExclusion NNP cord-104239-xxlcdbqi 125 5 Zone Zone NNP cord-104239-xxlcdbqi 125 6 . . . cord-104239-xxlcdbqi 126 1 The the DT cord-104239-xxlcdbqi 126 2 Golgi Golgi NNP cord-104239-xxlcdbqi 126 3 exclusion exclusion NN cord-104239-xxlcdbqi 126 4 zone zone NN cord-104239-xxlcdbqi 126 5 is be VBZ cord-104239-xxlcdbqi 126 6 defined define VBN cord-104239-xxlcdbqi 126 7 as as IN cord-104239-xxlcdbqi 126 8 the the DT cord-104239-xxlcdbqi 126 9 Golgi Golgi NNP cord-104239-xxlcdbqi 126 10 - - HYPH cord-104239-xxlcdbqi 126 11 containing contain VBG cord-104239-xxlcdbqi 126 12 region region NN cord-104239-xxlcdbqi 126 13 found find VBN cord-104239-xxlcdbqi 126 14 in in IN cord-104239-xxlcdbqi 126 15 the the DT cord-104239-xxlcdbqi 126 16 pericentrosomal pericentrosomal JJ cord-104239-xxlcdbqi 126 17 region region NN cord-104239-xxlcdbqi 126 18 of of IN cord-104239-xxlcdbqi 126 19 RBL rbl NN cord-104239-xxlcdbqi 126 20 cells cell NNS cord-104239-xxlcdbqi 126 21 and and CC cord-104239-xxlcdbqi 126 22 includes include VBZ cord-104239-xxlcdbqi 126 23 directly directly RB cord-104239-xxlcdbqi 126 24 adjacent adjacent JJ cord-104239-xxlcdbqi 126 25 bud bud NN cord-104239-xxlcdbqi 126 26 - - HYPH cord-104239-xxlcdbqi 126 27 bearing bear VBG cord-104239-xxlcdbqi 126 28 cisternae cisternae NN cord-104239-xxlcdbqi 126 29 of of IN cord-104239-xxlcdbqi 126 30 the the DT cord-104239-xxlcdbqi 126 31 ER ER NNP cord-104239-xxlcdbqi 126 32 . . . cord-104239-xxlcdbqi 127 1 The the DT cord-104239-xxlcdbqi 127 2 surface surface NN cord-104239-xxlcdbqi 127 3 area area NN cord-104239-xxlcdbqi 127 4 of of IN cord-104239-xxlcdbqi 127 5 the the DT cord-104239-xxlcdbqi 127 6 Golgi Golgi NNP cord-104239-xxlcdbqi 127 7 exclusion exclusion NN cord-104239-xxlcdbqi 127 8 zone zone NN cord-104239-xxlcdbqi 127 9 in in IN cord-104239-xxlcdbqi 127 10 each each DT cord-104239-xxlcdbqi 127 11 individual individual JJ cord-104239-xxlcdbqi 127 12 section section NN cord-104239-xxlcdbqi 127 13 within within IN cord-104239-xxlcdbqi 127 14 a a DT cord-104239-xxlcdbqi 127 15 stack stack NN cord-104239-xxlcdbqi 127 16 of of IN cord-104239-xxlcdbqi 127 17 11 11 CD cord-104239-xxlcdbqi 127 18 - - SYM cord-104239-xxlcdbqi 127 19 20 20 CD cord-104239-xxlcdbqi 127 20 consecutive consecutive JJ cord-104239-xxlcdbqi 127 21 sections section NNS cord-104239-xxlcdbqi 127 22 ( ( -LRB- cord-104239-xxlcdbqi 127 23 referred refer VBN cord-104239-xxlcdbqi 127 24 to to IN cord-104239-xxlcdbqi 127 25 as as IN cord-104239-xxlcdbqi 127 26 a a DT cord-104239-xxlcdbqi 127 27 disector disector NN cord-104239-xxlcdbqi 127 28 [ [ -LRB- cord-104239-xxlcdbqi 127 29 Sterio Sterio NNP cord-104239-xxlcdbqi 127 30 , , , cord-104239-xxlcdbqi 127 31 1984 1984 CD cord-104239-xxlcdbqi 127 32 ; ; : cord-104239-xxlcdbqi 127 33 Lucocq Lucocq NNP cord-104239-xxlcdbqi 127 34 et et FW cord-104239-xxlcdbqi 127 35 aL aL NNP cord-104239-xxlcdbqi 127 36 1989 1989 CD cord-104239-xxlcdbqi 127 37 ] ] -RRB- cord-104239-xxlcdbqi 127 38 ) ) -RRB- cord-104239-xxlcdbqi 127 39 was be VBD cord-104239-xxlcdbqi 127 40 determined determine VBN cord-104239-xxlcdbqi 127 41 by by IN cord-104239-xxlcdbqi 127 42 the the DT cord-104239-xxlcdbqi 127 43 point point NN cord-104239-xxlcdbqi 127 44 - - HYPH cord-104239-xxlcdbqi 127 45 counting counting NN cord-104239-xxlcdbqi 127 46 method method NN cord-104239-xxlcdbqi 127 47 ( ( -LRB- cord-104239-xxlcdbqi 127 48 Weibel Weibel NNP cord-104239-xxlcdbqi 127 49 , , , cord-104239-xxlcdbqi 127 50 1979 1979 CD cord-104239-xxlcdbqi 127 51 ) ) -RRB- cord-104239-xxlcdbqi 127 52 , , , cord-104239-xxlcdbqi 127 53 and and CC cord-104239-xxlcdbqi 127 54 the the DT cord-104239-xxlcdbqi 127 55 mean mean JJ cord-104239-xxlcdbqi 127 56 surface surface NN cord-104239-xxlcdbqi 127 57 area area NN cord-104239-xxlcdbqi 127 58 in in IN cord-104239-xxlcdbqi 127 59 each each DT cord-104239-xxlcdbqi 127 60 disector disector NN cord-104239-xxlcdbqi 127 61 was be VBD cord-104239-xxlcdbqi 127 62 calculated calculate VBN cord-104239-xxlcdbqi 127 63 for for IN cord-104239-xxlcdbqi 127 64 12 12 CD cord-104239-xxlcdbqi 127 65 different different JJ cord-104239-xxlcdbqi 127 66 cells cell NNS cord-104239-xxlcdbqi 127 67 ( ( -LRB- cord-104239-xxlcdbqi 127 68 range range VBP cord-104239-xxlcdbqi 127 69 from from IN cord-104239-xxlcdbqi 127 70 30 30 CD cord-104239-xxlcdbqi 127 71 - - SYM cord-104239-xxlcdbqi 127 72 90 90 CD cord-104239-xxlcdbqi 127 73 ~tm2 ~tm2 NNS cord-104239-xxlcdbqi 127 74 ) ) -RRB- cord-104239-xxlcdbqi 127 75 . . . cord-104239-xxlcdbqi 128 1 To to TO cord-104239-xxlcdbqi 128 2 find find VB cord-104239-xxlcdbqi 128 3 the the DT cord-104239-xxlcdbqi 128 4 volume volume NN cord-104239-xxlcdbqi 128 5 of of IN cord-104239-xxlcdbqi 128 6 the the DT cord-104239-xxlcdbqi 128 7 Golgi Golgi NNP cord-104239-xxlcdbqi 128 8 exclusion exclusion NN cord-104239-xxlcdbqi 128 9 zone zone NN cord-104239-xxlcdbqi 128 10 included include VBN cord-104239-xxlcdbqi 128 11 in in IN cord-104239-xxlcdbqi 128 12 the the DT cord-104239-xxlcdbqi 128 13 disector disector NN cord-104239-xxlcdbqi 128 14 , , , cord-104239-xxlcdbqi 128 15 the the DT cord-104239-xxlcdbqi 128 16 mean mean NN cord-104239-xxlcdbqi 128 17 surface surface NN cord-104239-xxlcdbqi 128 18 area area NN cord-104239-xxlcdbqi 128 19 of of IN cord-104239-xxlcdbqi 128 20 the the DT cord-104239-xxlcdbqi 128 21 Golgi Golgi NNP cord-104239-xxlcdbqi 128 22 exclusion exclusion NN cord-104239-xxlcdbqi 128 23 zone zone NN cord-104239-xxlcdbqi 128 24 in in IN cord-104239-xxlcdbqi 128 25 each each DT cord-104239-xxlcdbqi 128 26 disector disector NN cord-104239-xxlcdbqi 128 27 was be VBD cord-104239-xxlcdbqi 128 28 multiplied multiply VBN cord-104239-xxlcdbqi 128 29 by by IN cord-104239-xxlcdbqi 128 30 the the DT cord-104239-xxlcdbqi 128 31 thickness thickness NN cord-104239-xxlcdbqi 128 32 of of IN cord-104239-xxlcdbqi 128 33 the the DT cord-104239-xxlcdbqi 128 34 section section NN cord-104239-xxlcdbqi 128 35 and and CC cord-104239-xxlcdbqi 128 36 the the DT cord-104239-xxlcdbqi 128 37 number number NN cord-104239-xxlcdbqi 128 38 of of IN cord-104239-xxlcdbqi 128 39 individual individual JJ cord-104239-xxlcdbqi 128 40 sections section NNS cord-104239-xxlcdbqi 128 41 ( ( -LRB- cord-104239-xxlcdbqi 128 42 35 35 CD cord-104239-xxlcdbqi 128 43 to to TO cord-104239-xxlcdbqi 128 44 105 105 CD cord-104239-xxlcdbqi 128 45 ixm ixm NN cord-104239-xxlcdbqi 128 46 3 3 CD cord-104239-xxlcdbqi 128 47 for for IN cord-104239-xxlcdbqi 128 48 12 12 CD cord-104239-xxlcdbqi 128 49 individual individual JJ cord-104239-xxlcdbqi 128 50 disectors disector NNS cord-104239-xxlcdbqi 128 51 ) ) -RRB- cord-104239-xxlcdbqi 128 52 . . . cord-104239-xxlcdbqi 129 1 The the DT cord-104239-xxlcdbqi 129 2 total total JJ cord-104239-xxlcdbqi 129 3 number number NN cord-104239-xxlcdbqi 129 4 of of IN cord-104239-xxlcdbqi 129 5 ER ER NNP cord-104239-xxlcdbqi 129 6 buds bud NNS cord-104239-xxlcdbqi 129 7 within within IN cord-104239-xxlcdbqi 129 8 each each DT cord-104239-xxlcdbqi 129 9 disector disector NN cord-104239-xxlcdbqi 129 10 of of IN cord-104239-xxlcdbqi 129 11 a a DT cord-104239-xxlcdbqi 129 12 given give VBN cord-104239-xxlcdbqi 129 13 cell cell NN cord-104239-xxlcdbqi 129 14 was be VBD cord-104239-xxlcdbqi 129 15 found find VBN cord-104239-xxlcdbqi 129 16 by by IN cord-104239-xxlcdbqi 129 17 reconstruction reconstruction NN cord-104239-xxlcdbqi 129 18 from from IN cord-104239-xxlcdbqi 129 19 serial serial JJ cord-104239-xxlcdbqi 129 20 sections section NNS cord-104239-xxlcdbqi 129 21 . . . cord-104239-xxlcdbqi 130 1 The the DT cord-104239-xxlcdbqi 130 2 volumetric volumetric JJ cord-104239-xxlcdbqi 130 3 density density NN cord-104239-xxlcdbqi 130 4 of of IN cord-104239-xxlcdbqi 130 5 ER ER NNP cord-104239-xxlcdbqi 130 6 buds bud NNS cord-104239-xxlcdbqi 130 7 was be VBD cord-104239-xxlcdbqi 130 8 calculated calculate VBN cord-104239-xxlcdbqi 130 9 by by IN cord-104239-xxlcdbqi 130 10 dividing divide VBG cord-104239-xxlcdbqi 130 11 the the DT cord-104239-xxlcdbqi 130 12 number number NN cord-104239-xxlcdbqi 130 13 of of IN cord-104239-xxlcdbqi 130 14 buds bud NNS cord-104239-xxlcdbqi 130 15 found find VBN cord-104239-xxlcdbqi 130 16 within within IN cord-104239-xxlcdbqi 130 17 each each DT cord-104239-xxlcdbqi 130 18 disector disector NN cord-104239-xxlcdbqi 130 19 by by IN cord-104239-xxlcdbqi 130 20 the the DT cord-104239-xxlcdbqi 130 21 volume volume NN cord-104239-xxlcdbqi 130 22 of of IN cord-104239-xxlcdbqi 130 23 the the DT cord-104239-xxlcdbqi 130 24 disector disector NN cord-104239-xxlcdbqi 130 25 . . . cord-104239-xxlcdbqi 131 1 The the DT cord-104239-xxlcdbqi 131 2 total total JJ cord-104239-xxlcdbqi 131 3 volume volume NN cord-104239-xxlcdbqi 131 4 and and CC cord-104239-xxlcdbqi 131 5 surface surface NN cord-104239-xxlcdbqi 131 6 of of IN cord-104239-xxlcdbqi 131 7 ER ER NNP cord-104239-xxlcdbqi 131 8 membranes membrane NNS cord-104239-xxlcdbqi 131 9 in in IN cord-104239-xxlcdbqi 131 10 the the DT cord-104239-xxlcdbqi 131 11 Golgi Golgi NNP cord-104239-xxlcdbqi 131 12 exclusion exclusion NN cord-104239-xxlcdbqi 131 13 zone zone NN cord-104239-xxlcdbqi 131 14 was be VBD cord-104239-xxlcdbqi 131 15 found find VBN cord-104239-xxlcdbqi 131 16 by by IN cord-104239-xxlcdbqi 131 17 the the DT cord-104239-xxlcdbqi 131 18 point point NN cord-104239-xxlcdbqi 131 19 - - HYPH cord-104239-xxlcdbqi 131 20 counting counting NN cord-104239-xxlcdbqi 131 21 method method NN cord-104239-xxlcdbqi 131 22 ( ( -LRB- cord-104239-xxlcdbqi 131 23 Weibel Weibel NNP cord-104239-xxlcdbqi 131 24 , , , cord-104239-xxlcdbqi 131 25 1979 1979 CD cord-104239-xxlcdbqi 131 26 ) ) -RRB- cord-104239-xxlcdbqi 131 27 . . . cord-104239-xxlcdbqi 132 1 By by IN cord-104239-xxlcdbqi 132 2 dividing divide VBG cord-104239-xxlcdbqi 132 3 the the DT cord-104239-xxlcdbqi 132 4 total total JJ cord-104239-xxlcdbqi 132 5 number number NN cord-104239-xxlcdbqi 132 6 of of IN cord-104239-xxlcdbqi 132 7 ER ER NNP cord-104239-xxlcdbqi 132 8 buds bud NNS cord-104239-xxlcdbqi 132 9 by by IN cord-104239-xxlcdbqi 132 10 the the DT cord-104239-xxlcdbqi 132 11 ER ER NNP cord-104239-xxlcdbqi 132 12 surface surface NN cord-104239-xxlcdbqi 132 13 within within IN cord-104239-xxlcdbqi 132 14 the the DT cord-104239-xxlcdbqi 132 15 Golgi Golgi NNP cord-104239-xxlcdbqi 132 16 exclusion exclusion NN cord-104239-xxlcdbqi 132 17 zone zone NN cord-104239-xxlcdbqi 132 18 , , , cord-104239-xxlcdbqi 132 19 we -PRON- PRP cord-104239-xxlcdbqi 132 20 calculated calculate VBD cord-104239-xxlcdbqi 132 21 the the DT cord-104239-xxlcdbqi 132 22 density density NN cord-104239-xxlcdbqi 132 23 of of IN cord-104239-xxlcdbqi 132 24 ER ER NNP cord-104239-xxlcdbqi 132 25 buds bud NNS cord-104239-xxlcdbqi 132 26 in in IN cord-104239-xxlcdbqi 132 27 this this DT cord-104239-xxlcdbqi 132 28 region region NN cord-104239-xxlcdbqi 132 29 . . . cord-104239-xxlcdbqi 133 1 Buds bud NNS cord-104239-xxlcdbqi 133 2 outside outside RB cord-104239-xxlcdbqi 133 3 of of IN cord-104239-xxlcdbqi 133 4 the the DT cord-104239-xxlcdbqi 133 5 Golgi Golgi NNP cord-104239-xxlcdbqi 133 6 Exclusion Exclusion NNP cord-104239-xxlcdbqi 133 7 Zone Zone NNP cord-104239-xxlcdbqi 133 8 . . . cord-104239-xxlcdbqi 134 1 The the DT cord-104239-xxlcdbqi 134 2 density density NN cord-104239-xxlcdbqi 134 3 of of IN cord-104239-xxlcdbqi 134 4 ER ER NNP cord-104239-xxlcdbqi 134 5 buds bud NNS cord-104239-xxlcdbqi 134 6 outside outside IN cord-104239-xxlcdbqi 134 7 of of IN cord-104239-xxlcdbqi 134 8 the the DT cord-104239-xxlcdbqi 134 9 Golgi Golgi NNP cord-104239-xxlcdbqi 134 10 exclusion exclusion NN cord-104239-xxlcdbqi 134 11 zone zone NN cord-104239-xxlcdbqi 134 12 was be VBD cord-104239-xxlcdbqi 134 13 found find VBN cord-104239-xxlcdbqi 134 14 according accord VBG cord-104239-xxlcdbqi 134 15 to to IN cord-104239-xxlcdbqi 134 16 the the DT cord-104239-xxlcdbqi 134 17 procedure procedure NN cord-104239-xxlcdbqi 134 18 described describe VBN cord-104239-xxlcdbqi 134 19 above above RB cord-104239-xxlcdbqi 134 20 for for IN cord-104239-xxlcdbqi 134 21 the the DT cord-104239-xxlcdbqi 134 22 Golgi Golgi NNP cord-104239-xxlcdbqi 134 23 exclusion exclusion NN cord-104239-xxlcdbqi 134 24 zone zone NN cord-104239-xxlcdbqi 134 25 . . . cord-104239-xxlcdbqi 135 1 Buds bud NNS cord-104239-xxlcdbqi 135 2 in in IN cord-104239-xxlcdbqi 135 3 Export Export NNP cord-104239-xxlcdbqi 135 4 Complexes Complexes NNPS cord-104239-xxlcdbqi 135 5 . . . cord-104239-xxlcdbqi 136 1 The the DT cord-104239-xxlcdbqi 136 2 local local JJ cord-104239-xxlcdbqi 136 3 density density NN cord-104239-xxlcdbqi 136 4 of of IN cord-104239-xxlcdbqi 136 5 buds bud NNS cord-104239-xxlcdbqi 136 6 on on IN cord-104239-xxlcdbqi 136 7 individual individual JJ cord-104239-xxlcdbqi 136 8 cisternae cisternae NN cord-104239-xxlcdbqi 136 9 associated associate VBN cord-104239-xxlcdbqi 136 10 with with IN cord-104239-xxlcdbqi 136 11 export export NN cord-104239-xxlcdbqi 136 12 complexes complex NNS cord-104239-xxlcdbqi 136 13 was be VBD cord-104239-xxlcdbqi 136 14 determined determine VBN cord-104239-xxlcdbqi 136 15 as as IN cord-104239-xxlcdbqi 136 16 follows follow VBZ cord-104239-xxlcdbqi 136 17 : : : cord-104239-xxlcdbqi 136 18 using use VBG cord-104239-xxlcdbqi 136 19 a a DT cord-104239-xxlcdbqi 136 20 stack stack NN cord-104239-xxlcdbqi 136 21 of of IN cord-104239-xxlcdbqi 136 22 sequential sequential JJ cord-104239-xxlcdbqi 136 23 serial serial JJ cord-104239-xxlcdbqi 136 24 sections section NNS cord-104239-xxlcdbqi 136 25 , , , cord-104239-xxlcdbqi 136 26 we -PRON- PRP cord-104239-xxlcdbqi 136 27 followed follow VBD cord-104239-xxlcdbqi 136 28 one one CD cord-104239-xxlcdbqi 136 29 continuous continuous JJ cord-104239-xxlcdbqi 136 30 bud bud NN cord-104239-xxlcdbqi 136 31 - - HYPH cord-104239-xxlcdbqi 136 32 bearing bear VBG cord-104239-xxlcdbqi 136 33 zone zone NN cord-104239-xxlcdbqi 136 34 of of IN cord-104239-xxlcdbqi 136 35 ER ER NNP cord-104239-xxlcdbqi 136 36 membrane membrane NN cord-104239-xxlcdbqi 136 37 that that WDT cord-104239-xxlcdbqi 136 38 contained contain VBD cord-104239-xxlcdbqi 136 39 at at IN cord-104239-xxlcdbqi 136 40 least least JJS cord-104239-xxlcdbqi 136 41 four four CD cord-104239-xxlcdbqi 136 42 buds bud NNS cord-104239-xxlcdbqi 136 43 . . . cord-104239-xxlcdbqi 137 1 The the DT cord-104239-xxlcdbqi 137 2 distances distance NNS cord-104239-xxlcdbqi 137 3 between between IN cord-104239-xxlcdbqi 137 4 the the DT cord-104239-xxlcdbqi 137 5 most most RBS cord-104239-xxlcdbqi 137 6 distant distant JJ cord-104239-xxlcdbqi 137 7 ER ER NNP cord-104239-xxlcdbqi 137 8 buds bud NNS cord-104239-xxlcdbqi 137 9 in in IN cord-104239-xxlcdbqi 137 10 the the DT cord-104239-xxlcdbqi 137 11 stack stack NN cord-104239-xxlcdbqi 137 12 were be VBD cord-104239-xxlcdbqi 137 13 directly directly RB cord-104239-xxlcdbqi 137 14 measured measure VBN cord-104239-xxlcdbqi 137 15 in in IN cord-104239-xxlcdbqi 137 16 both both CC cord-104239-xxlcdbqi 137 17 the the DT cord-104239-xxlcdbqi 137 18 plane plane NN cord-104239-xxlcdbqi 137 19 of of IN cord-104239-xxlcdbqi 137 20 the the DT cord-104239-xxlcdbqi 137 21 section section NN cord-104239-xxlcdbqi 137 22 and and CC cord-104239-xxlcdbqi 137 23 the the DT cord-104239-xxlcdbqi 137 24 depth depth NN cord-104239-xxlcdbqi 137 25 of of IN cord-104239-xxlcdbqi 137 26 the the DT cord-104239-xxlcdbqi 137 27 stack stack NN cord-104239-xxlcdbqi 137 28 . . . cord-104239-xxlcdbqi 138 1 These these DT cord-104239-xxlcdbqi 138 2 two two CD cord-104239-xxlcdbqi 138 3 distances distance NNS cord-104239-xxlcdbqi 138 4 were be VBD cord-104239-xxlcdbqi 138 5 multiplied multiply VBN cord-104239-xxlcdbqi 138 6 by by IN cord-104239-xxlcdbqi 138 7 one one CD cord-104239-xxlcdbqi 138 8 another another DT cord-104239-xxlcdbqi 138 9 to to TO cord-104239-xxlcdbqi 138 10 get get VB cord-104239-xxlcdbqi 138 11 a a DT cord-104239-xxlcdbqi 138 12 surface surface NN cord-104239-xxlcdbqi 138 13 area area NN cord-104239-xxlcdbqi 138 14 of of IN cord-104239-xxlcdbqi 138 15 the the DT cord-104239-xxlcdbqi 138 16 plane plane NN cord-104239-xxlcdbqi 138 17 of of IN cord-104239-xxlcdbqi 138 18 the the DT cord-104239-xxlcdbqi 138 19 ER ER NNP cord-104239-xxlcdbqi 138 20 bud bud NN cord-104239-xxlcdbqi 138 21 - - HYPH cord-104239-xxlcdbqi 138 22 bearing bear VBG cord-104239-xxlcdbqi 138 23 region region NN cord-104239-xxlcdbqi 138 24 . . . cord-104239-xxlcdbqi 139 1 The the DT cord-104239-xxlcdbqi 139 2 total total JJ cord-104239-xxlcdbqi 139 3 number number NN cord-104239-xxlcdbqi 139 4 of of IN cord-104239-xxlcdbqi 139 5 ER ER NNP cord-104239-xxlcdbqi 139 6 buds bud NNS cord-104239-xxlcdbqi 139 7 in in IN cord-104239-xxlcdbqi 139 8 such such PDT cord-104239-xxlcdbqi 139 9 a a DT cord-104239-xxlcdbqi 139 10 zone zone NN cord-104239-xxlcdbqi 139 11 ( ( -LRB- cord-104239-xxlcdbqi 139 12 obtained obtain VBN cord-104239-xxlcdbqi 139 13 by by IN cord-104239-xxlcdbqi 139 14 reconstruction reconstruction NN cord-104239-xxlcdbqi 139 15 as as IN cord-104239-xxlcdbqi 139 16 described describe VBN cord-104239-xxlcdbqi 139 17 above above RB cord-104239-xxlcdbqi 139 18 for for IN cord-104239-xxlcdbqi 139 19 the the DT cord-104239-xxlcdbqi 139 20 Golgi Golgi NNP cord-104239-xxlcdbqi 139 21 exclusion exclusion NN cord-104239-xxlcdbqi 139 22 zone zone NN cord-104239-xxlcdbqi 139 23 ) ) -RRB- cord-104239-xxlcdbqi 139 24 was be VBD cord-104239-xxlcdbqi 139 25 then then RB cord-104239-xxlcdbqi 139 26 divided divide VBN cord-104239-xxlcdbqi 139 27 by by IN cord-104239-xxlcdbqi 139 28 the the DT cord-104239-xxlcdbqi 139 29 area area NN cord-104239-xxlcdbqi 139 30 of of IN cord-104239-xxlcdbqi 139 31 the the DT cord-104239-xxlcdbqi 139 32 bud bud NN cord-104239-xxlcdbqi 139 33 - - HYPH cord-104239-xxlcdbqi 139 34 bearing bear VBG cord-104239-xxlcdbqi 139 35 zone zone NN cord-104239-xxlcdbqi 139 36 to to TO cord-104239-xxlcdbqi 139 37 determine determine VB cord-104239-xxlcdbqi 139 38 a a DT cord-104239-xxlcdbqi 139 39 local local JJ cord-104239-xxlcdbqi 139 40 ER ER NNP cord-104239-xxlcdbqi 139 41 bud bud NN cord-104239-xxlcdbqi 139 42 surface surface NN cord-104239-xxlcdbqi 139 43 density density NN cord-104239-xxlcdbqi 139 44 . . . cord-104239-xxlcdbqi 140 1 Es.lmation Es.lmation NNP cord-104239-xxlcdbqi 140 2 of of IN cord-104239-xxlcdbqi 140 3 Number Number NNP cord-104239-xxlcdbqi 140 4 of of IN cord-104239-xxlcdbqi 140 5 VTCs vtc NNS cord-104239-xxlcdbqi 140 6 . . . cord-104239-xxlcdbqi 141 1 The the DT cord-104239-xxlcdbqi 141 2 number number NN cord-104239-xxlcdbqi 141 3 of of IN cord-104239-xxlcdbqi 141 4 VTCs vtc NNS cord-104239-xxlcdbqi 141 5 per per IN cord-104239-xxlcdbqi 141 6 cell cell NN cord-104239-xxlcdbqi 141 7 was be VBD cord-104239-xxlcdbqi 141 8 determined determine VBN cord-104239-xxlcdbqi 141 9 using use VBG cord-104239-xxlcdbqi 141 10 the the DT cord-104239-xxlcdbqi 141 11 disector disector NN cord-104239-xxlcdbqi 141 12 method method NN cord-104239-xxlcdbqi 141 13 ( ( -LRB- cord-104239-xxlcdbqi 141 14 Sterio Sterio NNP cord-104239-xxlcdbqi 141 15 , , , cord-104239-xxlcdbqi 141 16 1984 1984 CD cord-104239-xxlcdbqi 141 17 ) ) -RRB- cord-104239-xxlcdbqi 141 18 as as IN cord-104239-xxlcdbqi 141 19 described describe VBN cord-104239-xxlcdbqi 141 20 ( ( -LRB- cord-104239-xxlcdbqi 141 21 Lucocq Lucocq NNP cord-104239-xxlcdbqi 141 22 et et FW cord-104239-xxlcdbqi 141 23 al al NNP cord-104239-xxlcdbqi 141 24 . . NNP cord-104239-xxlcdbqi 141 25 , , , cord-104239-xxlcdbqi 141 26 1989 1989 CD cord-104239-xxlcdbqi 141 27 ) ) -RRB- cord-104239-xxlcdbqi 141 28 . . . cord-104239-xxlcdbqi 142 1 Sections section NNS cord-104239-xxlcdbqi 142 2 of of IN cord-104239-xxlcdbqi 142 3 26 26 CD cord-104239-xxlcdbqi 142 4 random random JJ cord-104239-xxlcdbqi 142 5 cells cell NNS cord-104239-xxlcdbqi 142 6 were be VBD cord-104239-xxlcdbqi 142 7 photographed photograph VBN cord-104239-xxlcdbqi 142 8 at at IN cord-104239-xxlcdbqi 142 9 a a DT cord-104239-xxlcdbqi 142 10 calibrated calibrate VBN cord-104239-xxlcdbqi 142 11 magnification magnification NN cord-104239-xxlcdbqi 142 12 of of IN cord-104239-xxlcdbqi 142 13 7,000 7,000 CD cord-104239-xxlcdbqi 142 14 throughout throughout IN cord-104239-xxlcdbqi 142 15 25 25 CD cord-104239-xxlcdbqi 142 16 - - SYM cord-104239-xxlcdbqi 142 17 35 35 CD cord-104239-xxlcdbqi 142 18 consecutive consecutive JJ cord-104239-xxlcdbqi 142 19 sections section NNS cord-104239-xxlcdbqi 142 20 in in IN cord-104239-xxlcdbqi 142 21 which which WDT cord-104239-xxlcdbqi 142 22 they -PRON- PRP cord-104239-xxlcdbqi 142 23 were be VBD cord-104239-xxlcdbqi 142 24 present present JJ cord-104239-xxlcdbqi 142 25 . . . cord-104239-xxlcdbqi 143 1 Each each DT cord-104239-xxlcdbqi 143 2 " " `` cord-104239-xxlcdbqi 143 3 end end NN cord-104239-xxlcdbqi 143 4 section section NN cord-104239-xxlcdbqi 143 5 " " '' cord-104239-xxlcdbqi 143 6 was be VBD cord-104239-xxlcdbqi 143 7 designated designate VBN cord-104239-xxlcdbqi 143 8 the the DT cord-104239-xxlcdbqi 143 9 " " `` cord-104239-xxlcdbqi 143 10 lookup lookup NN cord-104239-xxlcdbqi 143 11 " " '' cord-104239-xxlcdbqi 143 12 section section NN cord-104239-xxlcdbqi 143 13 , , , cord-104239-xxlcdbqi 143 14 and and CC cord-104239-xxlcdbqi 143 15 all all DT cord-104239-xxlcdbqi 143 16 clusters cluster NNS cord-104239-xxlcdbqi 143 17 present present JJ cord-104239-xxlcdbqi 143 18 in in IN cord-104239-xxlcdbqi 143 19 the the DT cord-104239-xxlcdbqi 143 20 other other JJ cord-104239-xxlcdbqi 143 21 sections section NNS cord-104239-xxlcdbqi 143 22 , , , cord-104239-xxlcdbqi 143 23 but but CC cord-104239-xxlcdbqi 143 24 not not RB cord-104239-xxlcdbqi 143 25 in in IN cord-104239-xxlcdbqi 143 26 the the DT cord-104239-xxlcdbqi 143 27 look look VB cord-104239-xxlcdbqi 143 28 - - HYPH cord-104239-xxlcdbqi 143 29 up up RP cord-104239-xxlcdbqi 143 30 section section NN cord-104239-xxlcdbqi 143 31 , , , cord-104239-xxlcdbqi 143 32 were be VBD cord-104239-xxlcdbqi 143 33 counted count VBN cord-104239-xxlcdbqi 143 34 ( ( -LRB- cord-104239-xxlcdbqi 143 35 Q Q NNP cord-104239-xxlcdbqi 143 36 ) ) -RRB- cord-104239-xxlcdbqi 143 37 . . . cord-104239-xxlcdbqi 144 1 Then then RB cord-104239-xxlcdbqi 144 2 the the DT cord-104239-xxlcdbqi 144 3 volume volume NN cord-104239-xxlcdbqi 144 4 of of IN cord-104239-xxlcdbqi 144 5 disector disector NN cord-104239-xxlcdbqi 144 6 ( ( -LRB- cord-104239-xxlcdbqi 144 7 Vdi~ Vdi~ NNP cord-104239-xxlcdbqi 144 8 ) ) -RRB- cord-104239-xxlcdbqi 144 9 was be VBD cord-104239-xxlcdbqi 144 10 determined determine VBN cord-104239-xxlcdbqi 144 11 by by IN cord-104239-xxlcdbqi 144 12 multiplying multiply VBG cord-104239-xxlcdbqi 144 13 the the DT cord-104239-xxlcdbqi 144 14 average average JJ cord-104239-xxlcdbqi 144 15 surface surface NN cord-104239-xxlcdbqi 144 16 of of IN cord-104239-xxlcdbqi 144 17 individual individual JJ cord-104239-xxlcdbqi 144 18 cells cell NNS cord-104239-xxlcdbqi 144 19 in in IN cord-104239-xxlcdbqi 144 20 the the DT cord-104239-xxlcdbqi 144 21 stack stack NN cord-104239-xxlcdbqi 144 22 found find VBN cord-104239-xxlcdbqi 144 23 by by IN cord-104239-xxlcdbqi 144 24 the the DT cord-104239-xxlcdbqi 144 25 point point NN cord-104239-xxlcdbqi 144 26 - - HYPH cord-104239-xxlcdbqi 144 27 counting counting NN cord-104239-xxlcdbqi 144 28 method method NN cord-104239-xxlcdbqi 144 29 by by IN cord-104239-xxlcdbqi 144 30 the the DT cord-104239-xxlcdbqi 144 31 depth depth NN cord-104239-xxlcdbqi 144 32 of of IN cord-104239-xxlcdbqi 144 33 the the DT cord-104239-xxlcdbqi 144 34 dissector dissector NN cord-104239-xxlcdbqi 144 35 , , , cord-104239-xxlcdbqi 144 36 which which WDT cord-104239-xxlcdbqi 144 37 is be VBZ cord-104239-xxlcdbqi 144 38 equal equal JJ cord-104239-xxlcdbqi 144 39 to to IN cord-104239-xxlcdbqi 144 40 the the DT cord-104239-xxlcdbqi 144 41 thickness thickness NN cord-104239-xxlcdbqi 144 42 of of IN cord-104239-xxlcdbqi 144 43 the the DT cord-104239-xxlcdbqi 144 44 section section NN cord-104239-xxlcdbqi 144 45 multiplied multiply VBN cord-104239-xxlcdbqi 144 46 by by IN cord-104239-xxlcdbqi 144 47 the the DT cord-104239-xxlcdbqi 144 48 number number NN cord-104239-xxlcdbqi 144 49 of of IN cord-104239-xxlcdbqi 144 50 sections section NNS cord-104239-xxlcdbqi 144 51 in in IN cord-104239-xxlcdbqi 144 52 the the DT cord-104239-xxlcdbqi 144 53 disector disector NN cord-104239-xxlcdbqi 144 54 . . . cord-104239-xxlcdbqi 145 1 The the DT cord-104239-xxlcdbqi 145 2 total total JJ cord-104239-xxlcdbqi 145 3 number number NN cord-104239-xxlcdbqi 145 4 of of IN cord-104239-xxlcdbqi 145 5 clusters cluster NNS cord-104239-xxlcdbqi 145 6 in in IN cord-104239-xxlcdbqi 145 7 individual individual JJ cord-104239-xxlcdbqi 145 8 ceils ceil NNS cord-104239-xxlcdbqi 146 1 ( ( -LRB- cord-104239-xxlcdbqi 146 2 Nclustcrs Nclustcrs NNP cord-104239-xxlcdbqi 146 3 / / SYM cord-104239-xxlcdbqi 146 4 cell cell NN cord-104239-xxlcdbqi 146 5 ) ) -RRB- cord-104239-xxlcdbqi 146 6 was be VBD cord-104239-xxlcdbqi 146 7 determined determine VBN cord-104239-xxlcdbqi 146 8 by by IN cord-104239-xxlcdbqi 146 9 the the DT cord-104239-xxlcdbqi 146 10 formula formula NN cord-104239-xxlcdbqi 146 11 , , , cord-104239-xxlcdbqi 146 12 ( ( -LRB- cord-104239-xxlcdbqi 146 13 4 4 LS cord-104239-xxlcdbqi 146 14 ) ) -RRB- cord-104239-xxlcdbqi 146 15 To to TO cord-104239-xxlcdbqi 146 16 determine determine VB cord-104239-xxlcdbqi 146 17 the the DT cord-104239-xxlcdbqi 146 18 number number NN cord-104239-xxlcdbqi 146 19 of of IN cord-104239-xxlcdbqi 146 20 elements element NNS cord-104239-xxlcdbqi 146 21 in in IN cord-104239-xxlcdbqi 146 22 VTCs vtc NNS cord-104239-xxlcdbqi 146 23 , , , cord-104239-xxlcdbqi 146 24 the the DT cord-104239-xxlcdbqi 146 25 value value NN cord-104239-xxlcdbqi 146 26 reported report VBD cord-104239-xxlcdbqi 146 27 assumes assume VBZ cord-104239-xxlcdbqi 146 28 that that IN cord-104239-xxlcdbqi 146 29 all all DT cord-104239-xxlcdbqi 146 30 elements element NNS cord-104239-xxlcdbqi 146 31 found find VBN cord-104239-xxlcdbqi 146 32 in in IN cord-104239-xxlcdbqi 146 33 consecutive consecutive JJ cord-104239-xxlcdbqi 146 34 sections section NNS cord-104239-xxlcdbqi 146 35 are be VBP cord-104239-xxlcdbqi 146 36 discontinuous discontinuous JJ cord-104239-xxlcdbqi 146 37 with with IN cord-104239-xxlcdbqi 146 38 one one CD cord-104239-xxlcdbqi 146 39 another another DT cord-104239-xxlcdbqi 146 40 . . . cord-104239-xxlcdbqi 147 1 This this DT cord-104239-xxlcdbqi 147 2 value value NN cord-104239-xxlcdbqi 147 3 is be VBZ cord-104239-xxlcdbqi 147 4 likely likely JJ cord-104239-xxlcdbqi 147 5 to to TO cord-104239-xxlcdbqi 147 6 be be VB cord-104239-xxlcdbqi 147 7 an an DT cord-104239-xxlcdbqi 147 8 overestimate overestimate JJ cord-104239-xxlcdbqi 147 9 , , , cord-104239-xxlcdbqi 147 10 as as IN cord-104239-xxlcdbqi 147 11 some some DT cord-104239-xxlcdbqi 147 12 of of IN cord-104239-xxlcdbqi 147 13 the the DT cord-104239-xxlcdbqi 147 14 tubular tubular JJ cord-104239-xxlcdbqi 147 15 structures structure NNS cord-104239-xxlcdbqi 147 16 within within IN cord-104239-xxlcdbqi 147 17 VTCs vtc NNS cord-104239-xxlcdbqi 147 18 may may MD cord-104239-xxlcdbqi 147 19 extend extend VB cord-104239-xxlcdbqi 147 20 across across IN cord-104239-xxlcdbqi 147 21 several several JJ cord-104239-xxlcdbqi 147 22 sections section NNS cord-104239-xxlcdbqi 147 23 . . . cord-104239-xxlcdbqi 148 1 The the DT cord-104239-xxlcdbqi 148 2 number number NN cord-104239-xxlcdbqi 148 3 of of IN cord-104239-xxlcdbqi 148 4 elements element NNS cord-104239-xxlcdbqi 148 5 in in IN cord-104239-xxlcdbqi 148 6 VTCs vtc NNS cord-104239-xxlcdbqi 148 7 was be VBD cord-104239-xxlcdbqi 148 8 determined determine VBN cord-104239-xxlcdbqi 148 9 by by IN cord-104239-xxlcdbqi 148 10 direct direct JJ cord-104239-xxlcdbqi 148 11 counting counting NN cord-104239-xxlcdbqi 148 12 of of IN cord-104239-xxlcdbqi 148 13 individual individual JJ cord-104239-xxlcdbqi 148 14 profiles profile NNS cord-104239-xxlcdbqi 148 15 on on IN cord-104239-xxlcdbqi 148 16 each each DT cord-104239-xxlcdbqi 148 17 serial serial JJ cord-104239-xxlcdbqi 148 18 section section NN cord-104239-xxlcdbqi 148 19 . . . cord-104239-xxlcdbqi 149 1 These these DT cord-104239-xxlcdbqi 149 2 were be VBD cord-104239-xxlcdbqi 149 3 summed sum VBN cord-104239-xxlcdbqi 149 4 throughout throughout IN cord-104239-xxlcdbqi 149 5 all all DT cord-104239-xxlcdbqi 149 6 sections section NNS cord-104239-xxlcdbqi 149 7 through through IN cord-104239-xxlcdbqi 149 8 a a DT cord-104239-xxlcdbqi 149 9 given give VBN cord-104239-xxlcdbqi 149 10 VTC vtc NN cord-104239-xxlcdbqi 149 11 to to TO cord-104239-xxlcdbqi 149 12 obtain obtain VB cord-104239-xxlcdbqi 149 13 a a DT cord-104239-xxlcdbqi 149 14 total total JJ cord-104239-xxlcdbqi 149 15 value value NN cord-104239-xxlcdbqi 149 16 . . . cord-104239-xxlcdbqi 150 1 Probability Probability NNP cord-104239-xxlcdbqi 150 2 Measurements measurement NNS cord-104239-xxlcdbqi 150 3 . . . cord-104239-xxlcdbqi 151 1 To to TO cord-104239-xxlcdbqi 151 2 estimate estimate VB cord-104239-xxlcdbqi 151 3 the the DT cord-104239-xxlcdbqi 151 4 probability probability NN cord-104239-xxlcdbqi 151 5 of of IN cord-104239-xxlcdbqi 151 6 a a DT cord-104239-xxlcdbqi 151 7 bud bud NN cord-104239-xxlcdbqi 151 8 having have VBG cord-104239-xxlcdbqi 151 9 proximity proximity NN cord-104239-xxlcdbqi 151 10 to to IN cord-104239-xxlcdbqi 151 11 a a DT cord-104239-xxlcdbqi 151 12 second second JJ cord-104239-xxlcdbqi 151 13 bud bud NN cord-104239-xxlcdbqi 151 14 in in IN cord-104239-xxlcdbqi 151 15 the the DT cord-104239-xxlcdbqi 151 16 cell cell NN cord-104239-xxlcdbqi 151 17 , , , cord-104239-xxlcdbqi 151 18 images image NNS cord-104239-xxlcdbqi 151 19 of of IN cord-104239-xxlcdbqi 151 20 serial serial JJ cord-104239-xxlcdbqi 151 21 sections section NNS cord-104239-xxlcdbqi 151 22 were be VBD cord-104239-xxlcdbqi 151 23 photoenlarged photoenlarge VBN cord-104239-xxlcdbqi 151 24 to to IN cord-104239-xxlcdbqi 151 25 40,000 40,000 CD cord-104239-xxlcdbqi 151 26 to to TO cord-104239-xxlcdbqi 151 27 reconstruct reconstruct VB cord-104239-xxlcdbqi 151 28 a a DT cord-104239-xxlcdbqi 151 29 section section NN cord-104239-xxlcdbqi 151 30 of of IN cord-104239-xxlcdbqi 151 31 the the DT cord-104239-xxlcdbqi 151 32 cell cell NN cord-104239-xxlcdbqi 151 33 in in IN cord-104239-xxlcdbqi 151 34 three three CD cord-104239-xxlcdbqi 151 35 dimensions dimension NNS cord-104239-xxlcdbqi 151 36 . . . cord-104239-xxlcdbqi 152 1 300 300 CD cord-104239-xxlcdbqi 152 2 randomly randomly RB cord-104239-xxlcdbqi 152 3 chosen choose VBN cord-104239-xxlcdbqi 152 4 ER ER NNP cord-104239-xxlcdbqi 152 5 buds bud NNS cord-104239-xxlcdbqi 152 6 were be VBD cord-104239-xxlcdbqi 152 7 taken take VBN cord-104239-xxlcdbqi 152 8 as as IN cord-104239-xxlcdbqi 152 9 the the DT cord-104239-xxlcdbqi 152 10 center center NN cord-104239-xxlcdbqi 152 11 of of IN cord-104239-xxlcdbqi 152 12 reference reference NN cord-104239-xxlcdbqi 152 13 ( ( -LRB- cord-104239-xxlcdbqi 152 14 referred refer VBN cord-104239-xxlcdbqi 152 15 to to IN cord-104239-xxlcdbqi 152 16 as as IN cord-104239-xxlcdbqi 152 17 reference reference NN cord-104239-xxlcdbqi 152 18 buds bud NNS cord-104239-xxlcdbqi 152 19 ) ) -RRB- cord-104239-xxlcdbqi 152 20 , , , cord-104239-xxlcdbqi 152 21 and and CC cord-104239-xxlcdbqi 152 22 the the DT cord-104239-xxlcdbqi 152 23 distance distance NN cord-104239-xxlcdbqi 152 24 between between IN cord-104239-xxlcdbqi 152 25 each each DT cord-104239-xxlcdbqi 152 26 individual individual JJ cord-104239-xxlcdbqi 152 27 reference reference NN cord-104239-xxlcdbqi 152 28 bud bud NN cord-104239-xxlcdbqi 152 29 and and CC cord-104239-xxlcdbqi 152 30 other other JJ cord-104239-xxlcdbqi 152 31 ER ER NNP cord-104239-xxlcdbqi 152 32 buds bud NNS cord-104239-xxlcdbqi 152 33 in in IN cord-104239-xxlcdbqi 152 34 the the DT cord-104239-xxlcdbqi 152 35 same same JJ cord-104239-xxlcdbqi 152 36 and and CC cord-104239-xxlcdbqi 152 37 consecutive consecutive JJ cord-104239-xxlcdbqi 152 38 serial serial JJ cord-104239-xxlcdbqi 152 39 sections section NNS cord-104239-xxlcdbqi 152 40 encompassing encompass VBG cord-104239-xxlcdbqi 152 41 up up IN cord-104239-xxlcdbqi 152 42 to to TO cord-104239-xxlcdbqi 152 43 1.3 1.3 CD cord-104239-xxlcdbqi 152 44 p.m p.m NN cord-104239-xxlcdbqi 152 45 distance distance NN cord-104239-xxlcdbqi 152 46 above above RB cord-104239-xxlcdbqi 152 47 and and CC cord-104239-xxlcdbqi 152 48 below below IN cord-104239-xxlcdbqi 152 49 the the DT cord-104239-xxlcdbqi 152 50 reference reference NN cord-104239-xxlcdbqi 152 51 bud bud NN cord-104239-xxlcdbqi 152 52 was be VBD cord-104239-xxlcdbqi 152 53 measured measure VBN cord-104239-xxlcdbqi 152 54 directly directly RB cord-104239-xxlcdbqi 152 55 . . . cord-104239-xxlcdbqi 153 1 A a DT cord-104239-xxlcdbqi 153 2 series series NN cord-104239-xxlcdbqi 153 3 of of IN cord-104239-xxlcdbqi 153 4 concentric concentric JJ cord-104239-xxlcdbqi 153 5 shells shell NNS cord-104239-xxlcdbqi 153 6 with with IN cord-104239-xxlcdbqi 153 7 a a DT cord-104239-xxlcdbqi 153 8 volume volume NN cord-104239-xxlcdbqi 153 9 equal equal JJ cord-104239-xxlcdbqi 153 10 to to IN cord-104239-xxlcdbqi 153 11 the the DT cord-104239-xxlcdbqi 153 12 volume volume NN cord-104239-xxlcdbqi 153 13 of of IN cord-104239-xxlcdbqi 153 14 the the DT cord-104239-xxlcdbqi 153 15 most most RBS cord-104239-xxlcdbqi 153 16 internal internal JJ cord-104239-xxlcdbqi 153 17 sphere sphere NN cord-104239-xxlcdbqi 153 18 having have VBG cord-104239-xxlcdbqi 153 19 a a DT cord-104239-xxlcdbqi 153 20 0.2 0.2 CD cord-104239-xxlcdbqi 153 21 - - HYPH cord-104239-xxlcdbqi 153 22 1xm 1xm JJ cord-104239-xxlcdbqi 153 23 diam diam NN cord-104239-xxlcdbqi 153 24 ( ( -LRB- cord-104239-xxlcdbqi 153 25 corresponding correspond VBG cord-104239-xxlcdbqi 153 26 to to IN cord-104239-xxlcdbqi 153 27 a a DT cord-104239-xxlcdbqi 153 28 volume volume NN cord-104239-xxlcdbqi 153 29 of of IN cord-104239-xxlcdbqi 153 30 0.0042 0.0042 CD cord-104239-xxlcdbqi 153 31 i i NN cord-104239-xxlcdbqi 153 32 ~ ~ CD cord-104239-xxlcdbqi 153 33 m m NN cord-104239-xxlcdbqi 153 34 3 3 CD cord-104239-xxlcdbqi 153 35 ) ) -RRB- cord-104239-xxlcdbqi 153 36 was be VBD cord-104239-xxlcdbqi 153 37 constructed construct VBN cord-104239-xxlcdbqi 153 38 around around IN cord-104239-xxlcdbqi 153 39 the the DT cord-104239-xxlcdbqi 153 40 reference reference NN cord-104239-xxlcdbqi 153 41 bud bud NN cord-104239-xxlcdbqi 153 42 . . . cord-104239-xxlcdbqi 154 1 Subsequently subsequently RB cord-104239-xxlcdbqi 154 2 , , , cord-104239-xxlcdbqi 154 3 each each DT cord-104239-xxlcdbqi 154 4 measured measure VBN cord-104239-xxlcdbqi 154 5 bud bud NN cord-104239-xxlcdbqi 154 6 was be VBD cord-104239-xxlcdbqi 154 7 assigned assign VBN cord-104239-xxlcdbqi 154 8 to to IN cord-104239-xxlcdbqi 154 9 a a DT cord-104239-xxlcdbqi 154 10 shell shell NN cord-104239-xxlcdbqi 154 11 with with IN cord-104239-xxlcdbqi 154 12 its -PRON- PRP$ cord-104239-xxlcdbqi 154 13 distance distance NN cord-104239-xxlcdbqi 154 14 from from IN cord-104239-xxlcdbqi 154 15 the the DT cord-104239-xxlcdbqi 154 16 reference reference NN cord-104239-xxlcdbqi 154 17 bud bud NN cord-104239-xxlcdbqi 154 18 being be VBG cord-104239-xxlcdbqi 154 19 that that DT cord-104239-xxlcdbqi 154 20 of of IN cord-104239-xxlcdbqi 154 21 the the DT cord-104239-xxlcdbqi 154 22 corresponding correspond VBG cord-104239-xxlcdbqi 154 23 outer outer JJ cord-104239-xxlcdbqi 154 24 diameter diameter NN cord-104239-xxlcdbqi 154 25 of of IN cord-104239-xxlcdbqi 154 26 the the DT cord-104239-xxlcdbqi 154 27 shell shell NN cord-104239-xxlcdbqi 154 28 . . . cord-104239-xxlcdbqi 155 1 The the DT cord-104239-xxlcdbqi 155 2 probability probability NN cord-104239-xxlcdbqi 155 3 of of IN cord-104239-xxlcdbqi 155 4 finding find VBG cord-104239-xxlcdbqi 155 5 a a DT cord-104239-xxlcdbqi 155 6 bud bud NN cord-104239-xxlcdbqi 155 7 within within IN cord-104239-xxlcdbqi 155 8 a a DT cord-104239-xxlcdbqi 155 9 particular particular JJ cord-104239-xxlcdbqi 155 10 shell shell NN cord-104239-xxlcdbqi 155 11 was be VBD cord-104239-xxlcdbqi 155 12 determined determine VBN cord-104239-xxlcdbqi 155 13 by by IN cord-104239-xxlcdbqi 155 14 dividing divide VBG cord-104239-xxlcdbqi 155 15 the the DT cord-104239-xxlcdbqi 155 16 number number NN cord-104239-xxlcdbqi 155 17 of of IN cord-104239-xxlcdbqi 155 18 positive positive JJ cord-104239-xxlcdbqi 155 19 shells shell NNS cord-104239-xxlcdbqi 155 20 by by IN cord-104239-xxlcdbqi 155 21 the the DT cord-104239-xxlcdbqi 155 22 number number NN cord-104239-xxlcdbqi 155 23 of of IN cord-104239-xxlcdbqi 155 24 reference reference NN cord-104239-xxlcdbqi 155 25 buds bud NNS cord-104239-xxlcdbqi 155 26 , , , cord-104239-xxlcdbqi 155 27 and and CC cord-104239-xxlcdbqi 155 28 the the DT cord-104239-xxlcdbqi 155 29 value value NN cord-104239-xxlcdbqi 155 30 was be VBD cord-104239-xxlcdbqi 155 31 reported report VBN cord-104239-xxlcdbqi 155 32 as as IN cord-104239-xxlcdbqi 155 33 a a DT cord-104239-xxlcdbqi 155 34 percentage percentage NN cord-104239-xxlcdbqi 155 35 . . . cord-104239-xxlcdbqi 156 1 SEM SEM NNP cord-104239-xxlcdbqi 156 2 . . . cord-104239-xxlcdbqi 157 1 Statistical statistical JJ cord-104239-xxlcdbqi 157 2 calculations calculation NNS cord-104239-xxlcdbqi 157 3 were be VBD cord-104239-xxlcdbqi 157 4 performed perform VBN cord-104239-xxlcdbqi 157 5 by by IN cord-104239-xxlcdbqi 157 6 determining determine VBG cord-104239-xxlcdbqi 157 7 the the DT cord-104239-xxlcdbqi 157 8 SEM SEM NNP cord-104239-xxlcdbqi 157 9 for for IN cord-104239-xxlcdbqi 157 10 the the DT cord-104239-xxlcdbqi 157 11 pooled pool VBN cord-104239-xxlcdbqi 157 12 stereological stereological JJ cord-104239-xxlcdbqi 157 13 data datum NNS cord-104239-xxlcdbqi 157 14 for for IN cord-104239-xxlcdbqi 157 15 each each DT cord-104239-xxlcdbqi 157 16 condition condition NN cord-104239-xxlcdbqi 157 17 as as IN cord-104239-xxlcdbqi 157 18 described describe VBN cord-104239-xxlcdbqi 157 19 in in IN cord-104239-xxlcdbqi 157 20 the the DT cord-104239-xxlcdbqi 157 21 Results result NNS cord-104239-xxlcdbqi 157 22 . . . cord-104239-xxlcdbqi 158 1 NRK NRK NNP cord-104239-xxlcdbqi 158 2 cells cell NNS cord-104239-xxlcdbqi 158 3 grown grow VBN cord-104239-xxlcdbqi 158 4 on on IN cord-104239-xxlcdbqi 158 5 35-mm 35-mm CD cord-104239-xxlcdbqi 158 6 tissue tissue NN cord-104239-xxlcdbqi 158 7 - - HYPH cord-104239-xxlcdbqi 158 8 culture culture NN cord-104239-xxlcdbqi 158 9 dishes dish NNS cord-104239-xxlcdbqi 158 10 were be VBD cord-104239-xxlcdbqi 158 11 infected infect VBN cord-104239-xxlcdbqi 158 12 with with IN cord-104239-xxlcdbqi 158 13 vesicular vesicular JJ cord-104239-xxlcdbqi 158 14 stomatitis stomatitis NN cord-104239-xxlcdbqi 158 15 virus virus NN cord-104239-xxlcdbqi 158 16 ( ( -LRB- cord-104239-xxlcdbqi 158 17 strain strain NN cord-104239-xxlcdbqi 158 18 ts045 ts045 IN cord-104239-xxlcdbqi 158 19 ) ) -RRB- cord-104239-xxlcdbqi 158 20 , , , cord-104239-xxlcdbqi 158 21 postinfected postinfecte VBN cord-104239-xxlcdbqi 158 22 for for IN cord-104239-xxlcdbqi 158 23 4 4 CD cord-104239-xxlcdbqi 158 24 h h NN cord-104239-xxlcdbqi 158 25 at at IN cord-104239-xxlcdbqi 158 26 39.5 39.5 CD cord-104239-xxlcdbqi 158 27 ° ° NN cord-104239-xxlcdbqi 158 28 C c NN cord-104239-xxlcdbqi 158 29 , , , cord-104239-xxlcdbqi 158 30 and and CC cord-104239-xxlcdbqi 158 31 permeabilized permeabilize VBD cord-104239-xxlcdbqi 158 32 as as IN cord-104239-xxlcdbqi 158 33 described describe VBN cord-104239-xxlcdbqi 158 34 ( ( -LRB- cord-104239-xxlcdbqi 158 35 Plutner Plutner NNP cord-104239-xxlcdbqi 158 36 et et NNP cord-104239-xxlcdbqi 158 37 al al NNP cord-104239-xxlcdbqi 158 38 .. .. NNP cord-104239-xxlcdbqi 158 39 1992 1992 CD cord-104239-xxlcdbqi 158 40 ) ) -RRB- cord-104239-xxlcdbqi 158 41 . . . cord-104239-xxlcdbqi 159 1 After after IN cord-104239-xxlcdbqi 159 2 incubation incubation NN cord-104239-xxlcdbqi 159 3 at at IN cord-104239-xxlcdbqi 159 4 the the DT cord-104239-xxlcdbqi 159 5 permissive permissive JJ cord-104239-xxlcdbqi 159 6 temperature temperature NN cord-104239-xxlcdbqi 159 7 ( ( -LRB- cord-104239-xxlcdbqi 159 8 32 32 CD cord-104239-xxlcdbqi 159 9 ° ° NN cord-104239-xxlcdbqi 159 10 C C NNP cord-104239-xxlcdbqi 159 11 ) ) -RRB- cord-104239-xxlcdbqi 159 12 as as IN cord-104239-xxlcdbqi 159 13 described describe VBN cord-104239-xxlcdbqi 159 14 in in IN cord-104239-xxlcdbqi 159 15 the the DT cord-104239-xxlcdbqi 159 16 Results result NNS cord-104239-xxlcdbqi 159 17 , , , cord-104239-xxlcdbqi 159 18 the the DT cord-104239-xxlcdbqi 159 19 cells cell NNS cord-104239-xxlcdbqi 159 20 were be VBD cord-104239-xxlcdbqi 159 21 fixed fix VBN cord-104239-xxlcdbqi 159 22 for for IN cord-104239-xxlcdbqi 159 23 30 30 CD cord-104239-xxlcdbqi 159 24 min min NN cord-104239-xxlcdbqi 159 25 with with IN cord-104239-xxlcdbqi 159 26 3 3 CD cord-104239-xxlcdbqi 159 27 % % NN cord-104239-xxlcdbqi 159 28 paraformaldehyde paraformaldehyde NN cord-104239-xxlcdbqi 159 29 and and CC cord-104239-xxlcdbqi 159 30 0.1 0.1 CD cord-104239-xxlcdbqi 159 31 % % NN cord-104239-xxlcdbqi 159 32 GA GA NNP cord-104239-xxlcdbqi 159 33 in in IN cord-104239-xxlcdbqi 159 34 PBS PBS NNP cord-104239-xxlcdbqi 159 35 ( ( -LRB- cord-104239-xxlcdbqi 159 36 pH pH NNP cord-104239-xxlcdbqi 159 37 7.4 7.4 CD cord-104239-xxlcdbqi 159 38 ) ) -RRB- cord-104239-xxlcdbqi 159 39 , , , cord-104239-xxlcdbqi 159 40 washed wash VBD cord-104239-xxlcdbqi 159 41 for for IN cord-104239-xxlcdbqi 159 42 l0 l0 NNP cord-104239-xxlcdbqi 159 43 rain rain NN cord-104239-xxlcdbqi 159 44 in in IN cord-104239-xxlcdbqi 159 45 PBS PBS NNP cord-104239-xxlcdbqi 159 46 containing contain VBG cord-104239-xxlcdbqi 159 47 0.05 0.05 CD cord-104239-xxlcdbqi 159 48 M M NNP cord-104239-xxlcdbqi 159 49 glycine glycine NN cord-104239-xxlcdbqi 159 50 , , , cord-104239-xxlcdbqi 159 51 scraped scrape VBN cord-104239-xxlcdbqi 159 52 , , , cord-104239-xxlcdbqi 159 53 mixed mix VBN cord-104239-xxlcdbqi 159 54 with with IN cord-104239-xxlcdbqi 159 55 a a DT cord-104239-xxlcdbqi 159 56 preheated preheated NN cord-104239-xxlcdbqi 159 57 ( ( -LRB- cord-104239-xxlcdbqi 159 58 40 40 CD cord-104239-xxlcdbqi 159 59 ° ° NN cord-104239-xxlcdbqi 159 60 C c NN cord-104239-xxlcdbqi 159 61 ) ) -RRB- cord-104239-xxlcdbqi 159 62 10 10 CD cord-104239-xxlcdbqi 159 63 % % NN cord-104239-xxlcdbqi 159 64 gelatin gelatin NN cord-104239-xxlcdbqi 159 65 in in IN cord-104239-xxlcdbqi 159 66 PBS PBS NNP cord-104239-xxlcdbqi 159 67 , , , cord-104239-xxlcdbqi 159 68 and and CC cord-104239-xxlcdbqi 159 69 centrifuged centrifuge VBD cord-104239-xxlcdbqi 159 70 at at IN cord-104239-xxlcdbqi 159 71 15,000 15,000 CD cord-104239-xxlcdbqi 159 72 g g NN cord-104239-xxlcdbqi 159 73 in in IN cord-104239-xxlcdbqi 159 74 a a DT cord-104239-xxlcdbqi 159 75 microcentrifuge microcentrifuge NN cord-104239-xxlcdbqi 159 76 for for IN cord-104239-xxlcdbqi 159 77 10 10 CD cord-104239-xxlcdbqi 159 78 min min NN cord-104239-xxlcdbqi 159 79 . . . cord-104239-xxlcdbqi 160 1 Cells cell NNS cord-104239-xxlcdbqi 160 2 embedded embed VBN cord-104239-xxlcdbqi 160 3 in in IN cord-104239-xxlcdbqi 160 4 gelatin gelatin NN cord-104239-xxlcdbqi 160 5 were be VBD cord-104239-xxlcdbqi 160 6 cooled cool VBN cord-104239-xxlcdbqi 160 7 on on IN cord-104239-xxlcdbqi 160 8 ice ice NN cord-104239-xxlcdbqi 160 9 , , , cord-104239-xxlcdbqi 160 10 and and CC cord-104239-xxlcdbqi 160 11 a a DT cord-104239-xxlcdbqi 160 12 solid solid JJ cord-104239-xxlcdbqi 160 13 pellet pellet NN cord-104239-xxlcdbqi 160 14 was be VBD cord-104239-xxlcdbqi 160 15 cut cut VBN cord-104239-xxlcdbqi 160 16 into into IN cord-104239-xxlcdbqi 160 17 I i NN cord-104239-xxlcdbqi 160 18 - - HYPH cord-104239-xxlcdbqi 160 19 ram ram NN cord-104239-xxlcdbqi 160 20 - - HYPH cord-104239-xxlcdbqi 160 21 wide wide JJ cord-104239-xxlcdbqi 160 22 cubes cube NNS cord-104239-xxlcdbqi 160 23 . . . cord-104239-xxlcdbqi 161 1 After after IN cord-104239-xxlcdbqi 161 2 overnight overnight JJ cord-104239-xxlcdbqi 161 3 cryoprotection cryoprotection NN cord-104239-xxlcdbqi 161 4 by by IN cord-104239-xxlcdbqi 161 5 infiltration infiltration NN cord-104239-xxlcdbqi 161 6 with with IN cord-104239-xxlcdbqi 161 7 a a DT cord-104239-xxlcdbqi 161 8 mixture mixture NN cord-104239-xxlcdbqi 161 9 of of IN cord-104239-xxlcdbqi 161 10 2.3 2.3 CD cord-104239-xxlcdbqi 161 11 M M NNP cord-104239-xxlcdbqi 161 12 sucrose sucrose NN cord-104239-xxlcdbqi 161 13 in in IN cord-104239-xxlcdbqi 161 14 0.1 0.1 CD cord-104239-xxlcdbqi 161 15 M m NN cord-104239-xxlcdbqi 161 16 phosphate phosphate NN cord-104239-xxlcdbqi 161 17 buffer buffer NN cord-104239-xxlcdbqi 161 18 ( ( -LRB- cord-104239-xxlcdbqi 161 19 pH pH NNP cord-104239-xxlcdbqi 161 20 7.4 7.4 CD cord-104239-xxlcdbqi 161 21 ) ) -RRB- cord-104239-xxlcdbqi 161 22 containing contain VBG cord-104239-xxlcdbqi 161 23 20 20 CD cord-104239-xxlcdbqi 161 24 % % NN cord-104239-xxlcdbqi 161 25 polyvinyl polyvinyl NN cord-104239-xxlcdbqi 161 26 pyrrolidene pyrrolidene NN cord-104239-xxlcdbqi 161 27 , , , cord-104239-xxlcdbqi 161 28 the the DT cord-104239-xxlcdbqi 161 29 cubes cube NNS cord-104239-xxlcdbqi 161 30 were be VBD cord-104239-xxlcdbqi 161 31 mounted mount VBN cord-104239-xxlcdbqi 161 32 on on IN cord-104239-xxlcdbqi 161 33 aluminum aluminum NN cord-104239-xxlcdbqi 161 34 nails nail NNS cord-104239-xxlcdbqi 161 35 and and CC cord-104239-xxlcdbqi 161 36 frozen freeze VBN cord-104239-xxlcdbqi 161 37 in in IN cord-104239-xxlcdbqi 161 38 liquid liquid JJ cord-104239-xxlcdbqi 161 39 nitrogen nitrogen NN cord-104239-xxlcdbqi 161 40 . . . cord-104239-xxlcdbqi 162 1 Ultrathin Ultrathin NNP cord-104239-xxlcdbqi 162 2 cryosections cryosection NNS cord-104239-xxlcdbqi 162 3 cut cut VBD cord-104239-xxlcdbqi 162 4 on on IN cord-104239-xxlcdbqi 162 5 a a DT cord-104239-xxlcdbqi 162 6 Reichert Reichert NNP cord-104239-xxlcdbqi 162 7 Ultracut Ultracut NNP cord-104239-xxlcdbqi 162 8 E E NNP cord-104239-xxlcdbqi 162 9 , , , cord-104239-xxlcdbqi 162 10 equipped equip VBN cord-104239-xxlcdbqi 162 11 with with IN cord-104239-xxlcdbqi 162 12 a a DT cord-104239-xxlcdbqi 162 13 FC-4 FC-4 NNP cord-104239-xxlcdbqi 162 14 cryoattachment cryoattachment NN cord-104239-xxlcdbqi 162 15 , , , cord-104239-xxlcdbqi 162 16 were be VBD cord-104239-xxlcdbqi 162 17 picked pick VBN cord-104239-xxlcdbqi 162 18 up up RP cord-104239-xxlcdbqi 162 19 with with IN cord-104239-xxlcdbqi 162 20 2.0 2.0 CD cord-104239-xxlcdbqi 162 21 M M NNP cord-104239-xxlcdbqi 162 22 sucrose-1 sucrose-1 NNP cord-104239-xxlcdbqi 162 23 % % NN cord-104239-xxlcdbqi 162 24 BSA BSA NNP cord-104239-xxlcdbqi 162 25 in in IN cord-104239-xxlcdbqi 162 26 PBS PBS NNP cord-104239-xxlcdbqi 162 27 and and CC cord-104239-xxlcdbqi 162 28 collected collect VBN cord-104239-xxlcdbqi 162 29 on on IN cord-104239-xxlcdbqi 162 30 Formvar Formvar NNP cord-104239-xxlcdbqi 162 31 / / SYM cord-104239-xxlcdbqi 162 32 carbon carbon NN cord-104239-xxlcdbqi 162 33 - - HYPH cord-104239-xxlcdbqi 162 34 coated coat VBN cord-104239-xxlcdbqi 162 35 nickel nickel NN cord-104239-xxlcdbqi 162 36 grids grid NNS cord-104239-xxlcdbqi 162 37 . . . cord-104239-xxlcdbqi 163 1 Sections section NNS cord-104239-xxlcdbqi 163 2 were be VBD cord-104239-xxlcdbqi 163 3 then then RB cord-104239-xxlcdbqi 163 4 quenched quench VBN cord-104239-xxlcdbqi 163 5 in in IN cord-104239-xxlcdbqi 163 6 0.01 0.01 CD cord-104239-xxlcdbqi 163 7 M m CD cord-104239-xxlcdbqi 163 8 glycine glycine NN cord-104239-xxlcdbqi 163 9 in in IN cord-104239-xxlcdbqi 163 10 PBS PBS NNP cord-104239-xxlcdbqi 163 11 , , , cord-104239-xxlcdbqi 163 12 incubated incubate VBN cord-104239-xxlcdbqi 163 13 for for IN cord-104239-xxlcdbqi 163 14 30 30 CD cord-104239-xxlcdbqi 163 15 min min NN cord-104239-xxlcdbqi 163 16 in in IN cord-104239-xxlcdbqi 163 17 10 10 CD cord-104239-xxlcdbqi 163 18 % % NN cord-104239-xxlcdbqi 163 19 FBS FBS NNP cord-104239-xxlcdbqi 163 20 - - HYPH cord-104239-xxlcdbqi 163 21 PBS PBS NNP cord-104239-xxlcdbqi 163 22 at at IN cord-104239-xxlcdbqi 163 23 rt rt NNP cord-104239-xxlcdbqi 163 24 , , , cord-104239-xxlcdbqi 163 25 and and CC cord-104239-xxlcdbqi 163 26 for for IN cord-104239-xxlcdbqi 163 27 1 1 CD cord-104239-xxlcdbqi 163 28 - - SYM cord-104239-xxlcdbqi 163 29 2 2 CD cord-104239-xxlcdbqi 163 30 h h NN cord-104239-xxlcdbqi 163 31 with with IN cord-104239-xxlcdbqi 163 32 primary primary JJ cord-104239-xxlcdbqi 163 33 antibodies antibody NNS cord-104239-xxlcdbqi 163 34 diluted dilute VBN cord-104239-xxlcdbqi 163 35 in in IN cord-104239-xxlcdbqi 163 36 10 10 CD cord-104239-xxlcdbqi 163 37 % % NN cord-104239-xxlcdbqi 163 38 FBS FBS NNP cord-104239-xxlcdbqi 163 39 - - HYPH cord-104239-xxlcdbqi 163 40 PBS PBS NNP cord-104239-xxlcdbqi 163 41 antibody antibody NN cord-104239-xxlcdbqi 163 42 . . . cord-104239-xxlcdbqi 164 1 Excess excess JJ cord-104239-xxlcdbqi 164 2 primary primary JJ cord-104239-xxlcdbqi 164 3 antibody antibody NN cord-104239-xxlcdbqi 164 4 was be VBD cord-104239-xxlcdbqi 164 5 removed remove VBN cord-104239-xxlcdbqi 164 6 by by IN cord-104239-xxlcdbqi 164 7 multiple multiple JJ cord-104239-xxlcdbqi 164 8 rinses rinse NNS cord-104239-xxlcdbqi 164 9 in in IN cord-104239-xxlcdbqi 164 10 5 5 CD cord-104239-xxlcdbqi 164 11 % % NN cord-104239-xxlcdbqi 164 12 FBS FBS NNP cord-104239-xxlcdbqi 164 13 - - HYPH cord-104239-xxlcdbqi 164 14 PBS PBS NNP cord-104239-xxlcdbqi 164 15 , , , cord-104239-xxlcdbqi 164 16 followed follow VBN cord-104239-xxlcdbqi 164 17 by by IN cord-104239-xxlcdbqi 164 18 transfer transfer NN cord-104239-xxlcdbqi 164 19 of of IN cord-104239-xxlcdbqi 164 20 the the DT cord-104239-xxlcdbqi 164 21 section section NN cord-104239-xxlcdbqi 164 22 to to IN cord-104239-xxlcdbqi 164 23 a a DT cord-104239-xxlcdbqi 164 24 drop drop NN cord-104239-xxlcdbqi 164 25 of of IN cord-104239-xxlcdbqi 164 26 10 10 CD cord-104239-xxlcdbqi 164 27 % % NN cord-104239-xxlcdbqi 165 1 FBS FBS NNP cord-104239-xxlcdbqi 165 2 - - HYPH cord-104239-xxlcdbqi 165 3 PBS PBS NNP cord-104239-xxlcdbqi 165 4 containing contain VBG cord-104239-xxlcdbqi 165 5 6 6 CD cord-104239-xxlcdbqi 165 6 or or CC cord-104239-xxlcdbqi 165 7 10 10 CD cord-104239-xxlcdbqi 165 8 nm nm NN cord-104239-xxlcdbqi 165 9 gold gold NN cord-104239-xxlcdbqi 165 10 -- -- : cord-104239-xxlcdbqi 165 11 conjugated conjugate VBN cord-104239-xxlcdbqi 165 12 anti anti JJ cord-104239-xxlcdbqi 165 13 - - JJ cord-104239-xxlcdbqi 165 14 rabbit rabbit JJ cord-104239-xxlcdbqi 165 15 antibodies antibody NNS cord-104239-xxlcdbqi 165 16 . . . cord-104239-xxlcdbqi 166 1 After after IN cord-104239-xxlcdbqi 166 2 a a DT cord-104239-xxlcdbqi 166 3 2-h 2-h CD cord-104239-xxlcdbqi 166 4 incubation incubation NN cord-104239-xxlcdbqi 166 5 at at IN cord-104239-xxlcdbqi 166 6 rt rt NNP cord-104239-xxlcdbqi 166 7 , , , cord-104239-xxlcdbqi 166 8 the the DT cord-104239-xxlcdbqi 166 9 grids grid NNS cord-104239-xxlcdbqi 166 10 were be VBD cord-104239-xxlcdbqi 166 11 washed wash VBN cord-104239-xxlcdbqi 166 12 in in IN cord-104239-xxlcdbqi 166 13 double double JJ cord-104239-xxlcdbqi 166 14 - - HYPH cord-104239-xxlcdbqi 166 15 distilled distilled JJ cord-104239-xxlcdbqi 166 16 water water NN cord-104239-xxlcdbqi 166 17 and and CC cord-104239-xxlcdbqi 166 18 stained stain VBN cord-104239-xxlcdbqi 166 19 in in IN cord-104239-xxlcdbqi 166 20 2 2 CD cord-104239-xxlcdbqi 166 21 % % NN cord-104239-xxlcdbqi 166 22 neutral neutral JJ cord-104239-xxlcdbqi 166 23 uranyl uranyl NN cord-104239-xxlcdbqi 166 24 acetate acetate NN cord-104239-xxlcdbqi 166 25 ( ( -LRB- cord-104239-xxlcdbqi 166 26 10 10 CD cord-104239-xxlcdbqi 166 27 rain rain NN cord-104239-xxlcdbqi 166 28 ) ) -RRB- cord-104239-xxlcdbqi 166 29 , , , cord-104239-xxlcdbqi 166 30 followed follow VBN cord-104239-xxlcdbqi 166 31 by by IN cord-104239-xxlcdbqi 166 32 embedment embedment NNP cord-104239-xxlcdbqi 166 33 in in IN cord-104239-xxlcdbqi 166 34 3.2 3.2 CD cord-104239-xxlcdbqi 166 35 % % NN cord-104239-xxlcdbqi 167 1 polyvinyl polyvinyl NN cord-104239-xxlcdbqi 167 2 alcohol/0.2 alcohol/0.2 IN cord-104239-xxlcdbqi 167 3 % % NN cord-104239-xxlcdbqi 167 4 methyl methyl NN cord-104239-xxlcdbqi 167 5 cellulose~ cellulose~ NNP cord-104239-xxlcdbqi 168 1 containing contain VBG cord-104239-xxlcdbqi 168 2 0.2 0.2 CD cord-104239-xxlcdbqi 168 3 % % NN cord-104239-xxlcdbqi 168 4 uranyl uranyl NN cord-104239-xxlcdbqi 168 5 acetate acetate NN cord-104239-xxlcdbqi 168 6 . . . cord-104239-xxlcdbqi 169 1 No no DT cord-104239-xxlcdbqi 169 2 labeling labeling NN cord-104239-xxlcdbqi 169 3 was be VBD cord-104239-xxlcdbqi 169 4 observed observe VBN cord-104239-xxlcdbqi 169 5 in in IN cord-104239-xxlcdbqi 169 6 controls control NNS cord-104239-xxlcdbqi 169 7 in in IN cord-104239-xxlcdbqi 169 8 which which WDT cord-104239-xxlcdbqi 169 9 primary primary JJ cord-104239-xxlcdbqi 169 10 antibodies antibody NNS cord-104239-xxlcdbqi 169 11 were be VBD cord-104239-xxlcdbqi 169 12 omitted omit VBN cord-104239-xxlcdbqi 169 13 . . . cord-104239-xxlcdbqi 170 1 NRK NRK NNP cord-104239-xxlcdbqi 170 2 cells cell NNS cord-104239-xxlcdbqi 170 3 were be VBD cord-104239-xxlcdbqi 170 4 infected infect VBN cord-104239-xxlcdbqi 170 5 with with IN cord-104239-xxlcdbqi 170 6 ts045 ts045 NNP cord-104239-xxlcdbqi 170 7 VSV VSV NNP cord-104239-xxlcdbqi 170 8 as as IN cord-104239-xxlcdbqi 170 9 described describe VBN cord-104239-xxlcdbqi 170 10 above above RB cord-104239-xxlcdbqi 170 11 . . . cord-104239-xxlcdbqi 171 1 After after IN cord-104239-xxlcdbqi 171 2 digitonin digitonin NNS cord-104239-xxlcdbqi 171 3 permeabilization permeabilization NN cord-104239-xxlcdbqi 171 4 ( ( -LRB- cord-104239-xxlcdbqi 171 5 Plutner Plutner NNP cord-104239-xxlcdbqi 171 6 et et NNP cord-104239-xxlcdbqi 171 7 al al NNP cord-104239-xxlcdbqi 171 8 . . NNP cord-104239-xxlcdbqi 171 9 , , , cord-104239-xxlcdbqi 171 10 1992 1992 CD cord-104239-xxlcdbqi 171 11 ) ) -RRB- cord-104239-xxlcdbqi 171 12 and and CC cord-104239-xxlcdbqi 171 13 incubation incubation NN cord-104239-xxlcdbqi 171 14 in in IN cord-104239-xxlcdbqi 171 15 vitro vitro FW cord-104239-xxlcdbqi 171 16 as as IN cord-104239-xxlcdbqi 171 17 described describe VBN cord-104239-xxlcdbqi 171 18 in in IN cord-104239-xxlcdbqi 171 19 the the DT cord-104239-xxlcdbqi 171 20 Results result NNS cord-104239-xxlcdbqi 171 21 , , , cord-104239-xxlcdbqi 171 22 cells cell NNS cord-104239-xxlcdbqi 171 23 were be VBD cord-104239-xxlcdbqi 171 24 fixed fix VBN cord-104239-xxlcdbqi 171 25 with with IN cord-104239-xxlcdbqi 171 26 0.025 0.025 CD cord-104239-xxlcdbqi 171 27 % % NN cord-104239-xxlcdbqi 171 28 GA/3 GA/3 NNP cord-104239-xxlcdbqi 171 29 % % NN cord-104239-xxlcdbqi 171 30 paraforrnaldehyde paraforrnaldehyde NNP cord-104239-xxlcdbqi 171 31 for for IN cord-104239-xxlcdbqi 171 32 30 30 CD cord-104239-xxlcdbqi 171 33 rain rain NN cord-104239-xxlcdbqi 171 34 . . . cord-104239-xxlcdbqi 172 1 Cells cell NNS cord-104239-xxlcdbqi 172 2 were be VBD cord-104239-xxlcdbqi 172 3 washed wash VBN cord-104239-xxlcdbqi 172 4 three three CD cord-104239-xxlcdbqi 172 5 times time NNS cord-104239-xxlcdbqi 172 6 with with IN cord-104239-xxlcdbqi 172 7 PBS PBS NNP cord-104239-xxlcdbqi 172 8 , , , cord-104239-xxlcdbqi 172 9 quenched quench VBN cord-104239-xxlcdbqi 172 10 with with IN cord-104239-xxlcdbqi 172 11 0.05 0.05 CD cord-104239-xxlcdbqi 172 12 M M NNP cord-104239-xxlcdbqi 172 13 glycine glycine NN cord-104239-xxlcdbqi 172 14 in in IN cord-104239-xxlcdbqi 172 15 10 10 CD cord-104239-xxlcdbqi 172 16 % % NN cord-104239-xxlcdbqi 172 17 FBS FBS NNP cord-104239-xxlcdbqi 172 18 / / SYM cord-104239-xxlcdbqi 172 19 PBS PBS NNP cord-104239-xxlcdbqi 172 20 for for IN cord-104239-xxlcdbqi 172 21 30 30 CD cord-104239-xxlcdbqi 172 22 rain rain NN cord-104239-xxlcdbqi 172 23 , , , cord-104239-xxlcdbqi 172 24 and and CC cord-104239-xxlcdbqi 172 25 incubated incubate VBN cord-104239-xxlcdbqi 172 26 overnight overnight RB cord-104239-xxlcdbqi 172 27 with with IN cord-104239-xxlcdbqi 172 28 an an DT cord-104239-xxlcdbqi 172 29 anti anti JJ cord-104239-xxlcdbqi 172 30 - - JJ cord-104239-xxlcdbqi 172 31 VSV vsv JJ cord-104239-xxlcdbqi 172 32 - - HYPH cord-104239-xxlcdbqi 172 33 G G NNP cord-104239-xxlcdbqi 172 34 cytoplasmic cytoplasmic JJ cord-104239-xxlcdbqi 172 35 tail tail NN cord-104239-xxlcdbqi 172 36 mAb mAb NNS cord-104239-xxlcdbqi 172 37 ( ( -LRB- cord-104239-xxlcdbqi 172 38 P5D4 P5D4 NNP cord-104239-xxlcdbqi 172 39 ) ) -RRB- cord-104239-xxlcdbqi 172 40 ( ( -LRB- cord-104239-xxlcdbqi 172 41 Kreis Kreis NNP cord-104239-xxlcdbqi 172 42 , , , cord-104239-xxlcdbqi 172 43 1986 1986 CD cord-104239-xxlcdbqi 172 44 ) ) -RRB- cord-104239-xxlcdbqi 172 45 . . . cord-104239-xxlcdbqi 173 1 Cells cell NNS cord-104239-xxlcdbqi 173 2 were be VBD cord-104239-xxlcdbqi 173 3 washed wash VBN cord-104239-xxlcdbqi 173 4 twice twice RB cord-104239-xxlcdbqi 173 5 with with IN cord-104239-xxlcdbqi 173 6 FBS FBS NNP cord-104239-xxlcdbqi 173 7 / / SYM cord-104239-xxlcdbqi 173 8 PBS PBS NNP cord-104239-xxlcdbqi 173 9 , , , cord-104239-xxlcdbqi 173 10 followed follow VBN cord-104239-xxlcdbqi 173 11 by by IN cord-104239-xxlcdbqi 173 12 incubation incubation NN cord-104239-xxlcdbqi 173 13 with with IN cord-104239-xxlcdbqi 173 14 rabbit rabbit NN cord-104239-xxlcdbqi 173 15 anti anti JJ cord-104239-xxlcdbqi 173 16 - - JJ cord-104239-xxlcdbqi 173 17 mouse mouse JJ cord-104239-xxlcdbqi 173 18 antibodies antibody NNS cord-104239-xxlcdbqi 173 19 for for IN cord-104239-xxlcdbqi 173 20 l-2 l-2 NNP cord-104239-xxlcdbqi 173 21 h h NNP cord-104239-xxlcdbqi 173 22 and and CC cord-104239-xxlcdbqi 173 23 with with IN cord-104239-xxlcdbqi 173 24 6 6 CD cord-104239-xxlcdbqi 173 25 or or CC cord-104239-xxlcdbqi 173 26 10 10 CD cord-104239-xxlcdbqi 173 27 nm nm NN cord-104239-xxlcdbqi 173 28 gold gold NN cord-104239-xxlcdbqi 173 29 conjugated conjugate VBN cord-104239-xxlcdbqi 173 30 to to IN cord-104239-xxlcdbqi 173 31 anti anti JJ cord-104239-xxlcdbqi 173 32 - - JJ cord-104239-xxlcdbqi 173 33 rabbit rabbit JJ cord-104239-xxlcdbqi 173 34 antibodies antibody NNS cord-104239-xxlcdbqi 173 35 for for IN cord-104239-xxlcdbqi 173 36 2 2 CD cord-104239-xxlcdbqi 173 37 h. h. NNP cord-104239-xxlcdbqi 173 38 Excess excess JJ cord-104239-xxlcdbqi 173 39 unbound unbound JJ cord-104239-xxlcdbqi 173 40 antibodies antibody NNS cord-104239-xxlcdbqi 173 41 were be VBD cord-104239-xxlcdbqi 173 42 removed remove VBN cord-104239-xxlcdbqi 173 43 by by IN cord-104239-xxlcdbqi 173 44 washing washing NN cord-104239-xxlcdbqi 173 45 , , , cord-104239-xxlcdbqi 173 46 and and CC cord-104239-xxlcdbqi 173 47 the the DT cord-104239-xxlcdbqi 173 48 cells cell NNS cord-104239-xxlcdbqi 173 49 were be VBD cord-104239-xxlcdbqi 173 50 fixed fix VBN cord-104239-xxlcdbqi 173 51 with with IN cord-104239-xxlcdbqi 173 52 2.5 2.5 CD cord-104239-xxlcdbqi 173 53 % % NN cord-104239-xxlcdbqi 173 54 GA GA NNP cord-104239-xxlcdbqi 173 55 for for IN cord-104239-xxlcdbqi 173 56 30 30 CD cord-104239-xxlcdbqi 173 57 rain rain NN cord-104239-xxlcdbqi 173 58 . . . cord-104239-xxlcdbqi 174 1 Cells cell NNS cord-104239-xxlcdbqi 174 2 were be VBD cord-104239-xxlcdbqi 174 3 scraped scrape VBN cord-104239-xxlcdbqi 174 4 , , , cord-104239-xxlcdbqi 174 5 pelleted pellete VBN cord-104239-xxlcdbqi 174 6 , , , cord-104239-xxlcdbqi 174 7 and and CC cord-104239-xxlcdbqi 174 8 processed process VBN cord-104239-xxlcdbqi 174 9 for for IN cord-104239-xxlcdbqi 174 10 Epon Epon NNP cord-104239-xxlcdbqi 174 11 embedding embedding NN cord-104239-xxlcdbqi 174 12 . . . cord-104239-xxlcdbqi 175 1 For for IN cord-104239-xxlcdbqi 175 2 three three CD cord-104239-xxlcdbqi 175 3 - - HYPH cord-104239-xxlcdbqi 175 4 dimensional dimensional JJ cord-104239-xxlcdbqi 175 5 visualization visualization NN cord-104239-xxlcdbqi 175 6 using use VBG cord-104239-xxlcdbqi 175 7 quick quick JJ cord-104239-xxlcdbqi 175 8 - - HYPH cord-104239-xxlcdbqi 175 9 freeze freeze JJ cord-104239-xxlcdbqi 175 10 , , , cord-104239-xxlcdbqi 175 11 deep deep JJ cord-104239-xxlcdbqi 175 12 etch etch NN cord-104239-xxlcdbqi 175 13 replicas replica NNS cord-104239-xxlcdbqi 175 14 , , , cord-104239-xxlcdbqi 175 15 immunolabeling immunolabele VBG cord-104239-xxlcdbqi 175 16 was be VBD cord-104239-xxlcdbqi 175 17 performed perform VBN cord-104239-xxlcdbqi 175 18 on on IN cord-104239-xxlcdbqi 175 19 glass glass NN cord-104239-xxlcdbqi 175 20 coverslips coverslip NNS cord-104239-xxlcdbqi 175 21 . . . cord-104239-xxlcdbqi 176 1 For for IN cord-104239-xxlcdbqi 176 2 quantitation quantitation NN cord-104239-xxlcdbqi 176 3 , , , cord-104239-xxlcdbqi 176 4 the the DT cord-104239-xxlcdbqi 176 5 Epon Epon NNP cord-104239-xxlcdbqi 176 6 - - HYPH cord-104239-xxlcdbqi 176 7 embedded embed VBN cord-104239-xxlcdbqi 176 8 cell cell NN cord-104239-xxlcdbqi 176 9 pellet pellet NN cord-104239-xxlcdbqi 176 10 was be VBD cord-104239-xxlcdbqi 176 11 cut cut VBN cord-104239-xxlcdbqi 176 12 as as IN cord-104239-xxlcdbqi 176 13 described describe VBN cord-104239-xxlcdbqi 176 14 above above RB cord-104239-xxlcdbqi 176 15 . . . cord-104239-xxlcdbqi 177 1 Images image NNS cord-104239-xxlcdbqi 177 2 ( ( -LRB- cord-104239-xxlcdbqi 177 3 xl0,000 xl0,000 MD cord-104239-xxlcdbqi 177 4 ) ) -RRB- cord-104239-xxlcdbqi 177 5 were be VBD cord-104239-xxlcdbqi 177 6 scanned scan VBN cord-104239-xxlcdbqi 177 7 into into IN cord-104239-xxlcdbqi 177 8 a a DT cord-104239-xxlcdbqi 177 9 computer computer NN cord-104239-xxlcdbqi 177 10 , , , cord-104239-xxlcdbqi 177 11 and and CC cord-104239-xxlcdbqi 177 12 membrane membrane NN cord-104239-xxlcdbqi 177 13 outlines outline NNS cord-104239-xxlcdbqi 177 14 of of IN cord-104239-xxlcdbqi 177 15 the the DT cord-104239-xxlcdbqi 177 16 ER ER NNP cord-104239-xxlcdbqi 177 17 and and CC cord-104239-xxlcdbqi 177 18 VTCs vtc NNS cord-104239-xxlcdbqi 177 19 were be VBD cord-104239-xxlcdbqi 177 20 measured measure VBN cord-104239-xxlcdbqi 177 21 using use VBG cord-104239-xxlcdbqi 177 22 the the DT cord-104239-xxlcdbqi 177 23 program program NN cord-104239-xxlcdbqi 177 24 NIH NIH NNP cord-104239-xxlcdbqi 177 25 Image Image NNP cord-104239-xxlcdbqi 177 26 , , , cord-104239-xxlcdbqi 177 27 version version NN cord-104239-xxlcdbqi 177 28 5.9 5.9 CD cord-104239-xxlcdbqi 177 29 . . . cord-104239-xxlcdbqi 178 1 The the DT cord-104239-xxlcdbqi 178 2 linear linear JJ cord-104239-xxlcdbqi 178 3 density density NN cord-104239-xxlcdbqi 178 4 of of IN cord-104239-xxlcdbqi 178 5 gold gold NN cord-104239-xxlcdbqi 178 6 particles particle NNS cord-104239-xxlcdbqi 178 7 corresponding correspond VBG cord-104239-xxlcdbqi 178 8 to to IN cord-104239-xxlcdbqi 178 9 VSV VSV NNP cord-104239-xxlcdbqi 178 10 - - HYPH cord-104239-xxlcdbqi 178 11 G G NNP cord-104239-xxlcdbqi 178 12 was be VBD cord-104239-xxlcdbqi 178 13 determined determine VBN cord-104239-xxlcdbqi 178 14 as as IN cord-104239-xxlcdbqi 178 15 described describe VBN cord-104239-xxlcdbqi 178 16 . . . cord-104239-xxlcdbqi 179 1 For for IN cord-104239-xxlcdbqi 179 2 determination determination NN cord-104239-xxlcdbqi 179 3 of of IN cord-104239-xxlcdbqi 179 4 the the DT cord-104239-xxlcdbqi 179 5 distribution distribution NN cord-104239-xxlcdbqi 179 6 of of IN cord-104239-xxlcdbqi 179 7 vesicles vesicle NNS cord-104239-xxlcdbqi 179 8 and and CC cord-104239-xxlcdbqi 179 9 VTCs vtc NNS cord-104239-xxlcdbqi 179 10 , , , cord-104239-xxlcdbqi 179 11 NRK NRK NNP cord-104239-xxlcdbqi 179 12 cells cell NNS cord-104239-xxlcdbqi 179 13 grown grow VBN cord-104239-xxlcdbqi 179 14 on on IN cord-104239-xxlcdbqi 179 15 glass glass NN cord-104239-xxlcdbqi 179 16 coverslips coverslip NNS cord-104239-xxlcdbqi 179 17 were be VBD cord-104239-xxlcdbqi 179 18 permeabilized permeabilize VBN cord-104239-xxlcdbqi 179 19 and and CC cord-104239-xxlcdbqi 179 20 incubated incubate VBN cord-104239-xxlcdbqi 179 21 as as IN cord-104239-xxlcdbqi 179 22 described describe VBN cord-104239-xxlcdbqi 179 23 in in IN cord-104239-xxlcdbqi 179 24 the the DT cord-104239-xxlcdbqi 179 25 Results result NNS cord-104239-xxlcdbqi 179 26 , , , cord-104239-xxlcdbqi 179 27 fixed fix VBN cord-104239-xxlcdbqi 179 28 and and CC cord-104239-xxlcdbqi 179 29 labeled label VBN cord-104239-xxlcdbqi 179 30 for for IN cord-104239-xxlcdbqi 179 31 VSV VSV NNP cord-104239-xxlcdbqi 179 32 - - HYPH cord-104239-xxlcdbqi 179 33 G G NNP cord-104239-xxlcdbqi 179 34 using use VBG cord-104239-xxlcdbqi 179 35 the the DT cord-104239-xxlcdbqi 179 36 immunodiffusion immunodiffusion NN cord-104239-xxlcdbqi 179 37 protocol protocol NN cord-104239-xxlcdbqi 179 38 , , , cord-104239-xxlcdbqi 179 39 and and CC cord-104239-xxlcdbqi 179 40 embedded embed VBN cord-104239-xxlcdbqi 179 41 in in IN cord-104239-xxlcdbqi 179 42 Epon Epon NNP cord-104239-xxlcdbqi 179 43 on on IN cord-104239-xxlcdbqi 179 44 the the DT cord-104239-xxlcdbqi 179 45 glass glass NN cord-104239-xxlcdbqi 179 46 coverslip coverslip NN cord-104239-xxlcdbqi 179 47 as as IN cord-104239-xxlcdbqi 179 48 described describe VBN cord-104239-xxlcdbqi 179 49 above above RB cord-104239-xxlcdbqi 179 50 . . . cord-104239-xxlcdbqi 180 1 After after IN cord-104239-xxlcdbqi 180 2 detachment detachment NN cord-104239-xxlcdbqi 180 3 from from IN cord-104239-xxlcdbqi 180 4 glass glass NN cord-104239-xxlcdbqi 180 5 , , , cord-104239-xxlcdbqi 180 6 thin thin JJ cord-104239-xxlcdbqi 180 7 layers layer NNS cord-104239-xxlcdbqi 180 8 of of IN cord-104239-xxlcdbqi 180 9 cells cell NNS cord-104239-xxlcdbqi 180 10 embedded embed VBN cord-104239-xxlcdbqi 180 11 in in IN cord-104239-xxlcdbqi 180 12 Epon Epon NNP cord-104239-xxlcdbqi 180 13 were be VBD cord-104239-xxlcdbqi 180 14 sandwiched sandwich VBN cord-104239-xxlcdbqi 180 15 against against IN cord-104239-xxlcdbqi 180 16 each each DT cord-104239-xxlcdbqi 180 17 other other JJ cord-104239-xxlcdbqi 180 18 and and CC cord-104239-xxlcdbqi 180 19 reembedded reembedde VBN cord-104239-xxlcdbqi 180 20 in in IN cord-104239-xxlcdbqi 180 21 Epon Epon NNP cord-104239-xxlcdbqi 180 22 . . . cord-104239-xxlcdbqi 181 1 Vertical vertical JJ cord-104239-xxlcdbqi 181 2 sections section NNS cord-104239-xxlcdbqi 181 3 of of IN cord-104239-xxlcdbqi 181 4 cells cell NNS cord-104239-xxlcdbqi 181 5 were be VBD cord-104239-xxlcdbqi 181 6 prepared prepare VBN cord-104239-xxlcdbqi 181 7 and and CC cord-104239-xxlcdbqi 181 8 counterstained counterstaine VBN cord-104239-xxlcdbqi 181 9 as as IN cord-104239-xxlcdbqi 181 10 described describe VBN cord-104239-xxlcdbqi 181 11 above above RB cord-104239-xxlcdbqi 181 12 . . . cord-104239-xxlcdbqi 182 1 ER ER NNP cord-104239-xxlcdbqi 182 2 to to IN cord-104239-xxlcdbqi 182 3 Golgi Golgi NNP cord-104239-xxlcdbqi 182 4 intermediates intermediate NNS cord-104239-xxlcdbqi 182 5 were be VBD cord-104239-xxlcdbqi 182 6 identified identify VBN cord-104239-xxlcdbqi 182 7 based base VBN cord-104239-xxlcdbqi 182 8 on on IN cord-104239-xxlcdbqi 182 9 either either CC cord-104239-xxlcdbqi 182 10 the the DT cord-104239-xxlcdbqi 182 11 presence presence NN cord-104239-xxlcdbqi 182 12 of of IN cord-104239-xxlcdbqi 182 13 gold gold NN cord-104239-xxlcdbqi 182 14 particles particle NNS cord-104239-xxlcdbqi 182 15 corresponding correspond VBG cord-104239-xxlcdbqi 182 16 to to IN cord-104239-xxlcdbqi 182 17 VSV VSV NNP cord-104239-xxlcdbqi 182 18 - - HYPH cord-104239-xxlcdbqi 182 19 G G NNP cord-104239-xxlcdbqi 182 20 or or CC cord-104239-xxlcdbqi 182 21 on on IN cord-104239-xxlcdbqi 182 22 their -PRON- PRP$ cord-104239-xxlcdbqi 182 23 characteristic characteristic JJ cord-104239-xxlcdbqi 182 24 morphology morphology NN cord-104239-xxlcdbqi 182 25 as as IN cord-104239-xxlcdbqi 182 26 described describe VBN cord-104239-xxlcdbqi 182 27 in in IN cord-104239-xxlcdbqi 182 28 the the DT cord-104239-xxlcdbqi 182 29 Results result NNS cord-104239-xxlcdbqi 182 30 . . . cord-104239-xxlcdbqi 183 1 After after IN cord-104239-xxlcdbqi 183 2 incubation incubation NN cord-104239-xxlcdbqi 183 3 in in IN cord-104239-xxlcdbqi 183 4 vitro vitro FW cord-104239-xxlcdbqi 183 5 as as IN cord-104239-xxlcdbqi 183 6 indicated indicate VBN cord-104239-xxlcdbqi 183 7 in in IN cord-104239-xxlcdbqi 183 8 the the DT cord-104239-xxlcdbqi 183 9 Results result NNS cord-104239-xxlcdbqi 183 10 , , , cord-104239-xxlcdbqi 183 11 semi semi JJ cord-104239-xxlcdbqi 183 12 - - JJ cord-104239-xxlcdbqi 183 13 intact intact JJ cord-104239-xxlcdbqi 183 14 cells cell NNS cord-104239-xxlcdbqi 183 15 grown grow VBN cord-104239-xxlcdbqi 183 16 on on IN cord-104239-xxlcdbqi 183 17 9 9 CD cord-104239-xxlcdbqi 183 18 × × NN cord-104239-xxlcdbqi 183 19 9 9 CD cord-104239-xxlcdbqi 183 20 mm mm NNP cord-104239-xxlcdbqi 183 21 glass glass NN cord-104239-xxlcdbqi 183 22 coverslips coverslip NNS cord-104239-xxlcdbqi 183 23 were be VBD cord-104239-xxlcdbqi 183 24 fixed fix VBN cord-104239-xxlcdbqi 183 25 in in IN cord-104239-xxlcdbqi 183 26 2.5 2.5 CD cord-104239-xxlcdbqi 183 27 % % NN cord-104239-xxlcdbqi 183 28 GA GA NNP cord-104239-xxlcdbqi 183 29 in in IN cord-104239-xxlcdbqi 183 30 PBS PBS NNP cord-104239-xxlcdbqi 183 31 for for IN cord-104239-xxlcdbqi 183 32 1 1 CD cord-104239-xxlcdbqi 183 33 h h NN cord-104239-xxlcdbqi 183 34 , , , cord-104239-xxlcdbqi 183 35 washed wash VBN cord-104239-xxlcdbqi 183 36 in in IN cord-104239-xxlcdbqi 183 37 PBS PBS NNP cord-104239-xxlcdbqi 183 38 , , , cord-104239-xxlcdbqi 183 39 and and CC cord-104239-xxlcdbqi 183 40 divided divide VBN cord-104239-xxlcdbqi 183 41 into into IN cord-104239-xxlcdbqi 183 42 small small JJ cord-104239-xxlcdbqi 183 43 pieces piece NNS cord-104239-xxlcdbqi 183 44 ( ( -LRB- cord-104239-xxlcdbqi 183 45 ~3 ~3 NFP cord-104239-xxlcdbqi 183 46 mm2 mm2 NNP cord-104239-xxlcdbqi 183 47 ) ) -RRB- cord-104239-xxlcdbqi 183 48 . . . cord-104239-xxlcdbqi 184 1 Coverslips coverslip NNS cord-104239-xxlcdbqi 184 2 were be VBD cord-104239-xxlcdbqi 184 3 rinsed rinse VBN cord-104239-xxlcdbqi 184 4 exhaustively exhaustively RB cord-104239-xxlcdbqi 184 5 in in IN cord-104239-xxlcdbqi 184 6 double double JJ cord-104239-xxlcdbqi 184 7 - - HYPH cord-104239-xxlcdbqi 184 8 distilled distilled JJ cord-104239-xxlcdbqi 184 9 water water NN cord-104239-xxlcdbqi 184 10 , , , cord-104239-xxlcdbqi 184 11 followed follow VBN cord-104239-xxlcdbqi 184 12 by by IN cord-104239-xxlcdbqi 184 13 a a DT cord-104239-xxlcdbqi 184 14 rinse rinse NN cord-104239-xxlcdbqi 184 15 with with IN cord-104239-xxlcdbqi 184 16 10 10 CD cord-104239-xxlcdbqi 184 17 % % NN cord-104239-xxlcdbqi 184 18 methanol methanol NN cord-104239-xxlcdbqi 184 19 in in IN cord-104239-xxlcdbqi 184 20 water water NN cord-104239-xxlcdbqi 184 21 , , , cord-104239-xxlcdbqi 184 22 and and CC cord-104239-xxlcdbqi 184 23 quick quick RB cord-104239-xxlcdbqi 184 24 - - HYPH cord-104239-xxlcdbqi 184 25 frozen frozen JJ cord-104239-xxlcdbqi 184 26 using use VBG cord-104239-xxlcdbqi 184 27 a a DT cord-104239-xxlcdbqi 184 28 liquid liquid JJ cord-104239-xxlcdbqi 184 29 nitrogen nitrogen NN cord-104239-xxlcdbqi 184 30 - - HYPH cord-104239-xxlcdbqi 184 31 cooled cool VBN cord-104239-xxlcdbqi 184 32 copper copper NN cord-104239-xxlcdbqi 184 33 block block NN cord-104239-xxlcdbqi 184 34 gravity gravity NN cord-104239-xxlcdbqi 184 35 press press NN cord-104239-xxlcdbqi 184 36 ( ( -LRB- cord-104239-xxlcdbqi 184 37 Hitek Hitek NNP cord-104239-xxlcdbqi 184 38 , , , cord-104239-xxlcdbqi 184 39 Benicia Benicia NNP cord-104239-xxlcdbqi 184 40 , , , cord-104239-xxlcdbqi 184 41 CA CA NNP cord-104239-xxlcdbqi 184 42 ) ) -RRB- cord-104239-xxlcdbqi 184 43 . . . cord-104239-xxlcdbqi 185 1 The the DT cord-104239-xxlcdbqi 185 2 cells cell NNS cord-104239-xxlcdbqi 185 3 were be VBD cord-104239-xxlcdbqi 185 4 fractured fracture VBN cord-104239-xxlcdbqi 185 5 with with IN cord-104239-xxlcdbqi 185 6 a a DT cord-104239-xxlcdbqi 185 7 razor razor NN cord-104239-xxlcdbqi 185 8 blade blade NN cord-104239-xxlcdbqi 185 9 under under IN cord-104239-xxlcdbqi 185 10 liquid liquid JJ cord-104239-xxlcdbqi 185 11 nitrogen nitrogen NN cord-104239-xxlcdbqi 185 12 , , , cord-104239-xxlcdbqi 185 13 freeze freeze VB cord-104239-xxlcdbqi 185 14 dried dry VBN cord-104239-xxlcdbqi 185 15 in in IN cord-104239-xxlcdbqi 185 16 a a DT cord-104239-xxlcdbqi 185 17 vacuum vacuum NN cord-104239-xxlcdbqi 185 18 evaporator evaporator NN cord-104239-xxlcdbqi 185 19 ( ( -LRB- cord-104239-xxlcdbqi 185 20 400 400 CD cord-104239-xxlcdbqi 185 21 ; ; : cord-104239-xxlcdbqi 185 22 Balzers Balzers NNP cord-104239-xxlcdbqi 185 23 , , , cord-104239-xxlcdbqi 185 24 Inc. Inc. NNP cord-104239-xxlcdbqi 185 25 , , , cord-104239-xxlcdbqi 185 26 Lichtenstein Lichtenstein NNP cord-104239-xxlcdbqi 185 27 ) ) -RRB- cord-104239-xxlcdbqi 185 28 and and CC cord-104239-xxlcdbqi 185 29 replicated replicate VBD cord-104239-xxlcdbqi 185 30 with with IN cord-104239-xxlcdbqi 185 31 ~2 ~2 NNP cord-104239-xxlcdbqi 185 32 nm nm NN cord-104239-xxlcdbqi 185 33 of of IN cord-104239-xxlcdbqi 185 34 platinum platinum NN cord-104239-xxlcdbqi 185 35 that that WDT cord-104239-xxlcdbqi 185 36 was be VBD cord-104239-xxlcdbqi 185 37 rotary rotary RB cord-104239-xxlcdbqi 185 38 deposited deposit VBN cord-104239-xxlcdbqi 185 39 from from IN cord-104239-xxlcdbqi 185 40 24 24 CD cord-104239-xxlcdbqi 185 41 ° ° NNS cord-104239-xxlcdbqi 185 42 above above IN cord-104239-xxlcdbqi 185 43 the the DT cord-104239-xxlcdbqi 185 44 horizontal horizontal NN cord-104239-xxlcdbqi 185 45 . . . cord-104239-xxlcdbqi 186 1 The the DT cord-104239-xxlcdbqi 186 2 replica replica NN cord-104239-xxlcdbqi 186 3 was be VBD cord-104239-xxlcdbqi 186 4 then then RB cord-104239-xxlcdbqi 186 5 reinforced reinforce VBN cord-104239-xxlcdbqi 186 6 with with IN cord-104239-xxlcdbqi 186 7 ~140/~ ~140/~ NN cord-104239-xxlcdbqi 186 8 of of IN cord-104239-xxlcdbqi 186 9 carbon carbon NN cord-104239-xxlcdbqi 186 10 using use VBG cord-104239-xxlcdbqi 186 11 an an DT cord-104239-xxlcdbqi 186 12 electron electron NN cord-104239-xxlcdbqi 186 13 gun gun NN cord-104239-xxlcdbqi 186 14 at at IN cord-104239-xxlcdbqi 186 15 an an DT cord-104239-xxlcdbqi 186 16 angle angle NN cord-104239-xxlcdbqi 186 17 of of IN cord-104239-xxlcdbqi 186 18 90 90 CD cord-104239-xxlcdbqi 186 19 ° ° NNS cord-104239-xxlcdbqi 186 20 to to IN cord-104239-xxlcdbqi 186 21 the the DT cord-104239-xxlcdbqi 186 22 horizontal horizontal NN cord-104239-xxlcdbqi 186 23 . . . cord-104239-xxlcdbqi 187 1 A a DT cord-104239-xxlcdbqi 187 2 drop drop NN cord-104239-xxlcdbqi 187 3 of of IN cord-104239-xxlcdbqi 187 4 2 2 CD cord-104239-xxlcdbqi 187 5 % % NN cord-104239-xxlcdbqi 187 6 colloidion colloidion NN cord-104239-xxlcdbqi 187 7 solution solution NN cord-104239-xxlcdbqi 187 8 was be VBD cord-104239-xxlcdbqi 187 9 applied apply VBN cord-104239-xxlcdbqi 187 10 on on IN cord-104239-xxlcdbqi 187 11 the the DT cord-104239-xxlcdbqi 187 12 replica replica JJ cord-104239-xxlcdbqi 187 13 membrane membrane NN cord-104239-xxlcdbqi 187 14 . . . cord-104239-xxlcdbqi 188 1 The the DT cord-104239-xxlcdbqi 188 2 coverslips coverslip NNS cord-104239-xxlcdbqi 188 3 were be VBD cord-104239-xxlcdbqi 188 4 detached detach VBN cord-104239-xxlcdbqi 188 5 in in IN cord-104239-xxlcdbqi 188 6 a a DT cord-104239-xxlcdbqi 188 7 40 40 CD cord-104239-xxlcdbqi 188 8 % % NN cord-104239-xxlcdbqi 188 9 solution solution NN cord-104239-xxlcdbqi 188 10 of of IN cord-104239-xxlcdbqi 188 11 hydrofluoric hydrofluoric JJ cord-104239-xxlcdbqi 188 12 acid acid NN cord-104239-xxlcdbqi 188 13 , , , cord-104239-xxlcdbqi 188 14 and and CC cord-104239-xxlcdbqi 188 15 cells cell NNS cord-104239-xxlcdbqi 188 16 were be VBD cord-104239-xxlcdbqi 188 17 dissolved dissolve VBN cord-104239-xxlcdbqi 188 18 in in IN cord-104239-xxlcdbqi 188 19 Chlorox Chlorox NNP cord-104239-xxlcdbqi 188 20 . . . cord-104239-xxlcdbqi 189 1 After after IN cord-104239-xxlcdbqi 189 2 washing washing NN cord-104239-xxlcdbqi 189 3 , , , cord-104239-xxlcdbqi 189 4 replicas replica NNS cord-104239-xxlcdbqi 189 5 were be VBD cord-104239-xxlcdbqi 189 6 transferred transfer VBN cord-104239-xxlcdbqi 189 7 to to IN cord-104239-xxlcdbqi 189 8 Formvar Formvar NNP cord-104239-xxlcdbqi 189 9 - - HYPH cord-104239-xxlcdbqi 189 10 coated coat VBN cord-104239-xxlcdbqi 189 11 copper copper NN cord-104239-xxlcdbqi 189 12 grids grid NNS cord-104239-xxlcdbqi 189 13 , , , cord-104239-xxlcdbqi 189 14 the the DT cord-104239-xxlcdbqi 189 15 colloidion colloidion NN cord-104239-xxlcdbqi 189 16 film film NN cord-104239-xxlcdbqi 189 17 on on IN cord-104239-xxlcdbqi 189 18 replicas replica NNS cord-104239-xxlcdbqi 189 19 was be VBD cord-104239-xxlcdbqi 189 20 dissolved dissolve VBN cord-104239-xxlcdbqi 189 21 with with IN cord-104239-xxlcdbqi 189 22 amylacetate amylacetate NNP cord-104239-xxlcdbqi 189 23 , , , cord-104239-xxlcdbqi 189 24 and and CC cord-104239-xxlcdbqi 189 25 images image NNS cord-104239-xxlcdbqi 189 26 were be VBD cord-104239-xxlcdbqi 189 27 examined examine VBN cord-104239-xxlcdbqi 189 28 using use VBG cord-104239-xxlcdbqi 189 29 TEM tem NN cord-104239-xxlcdbqi 189 30 . . . cord-104239-xxlcdbqi 190 1 The the DT cord-104239-xxlcdbqi 190 2 basic basic JJ cord-104239-xxlcdbqi 190 3 stereological stereological JJ cord-104239-xxlcdbqi 190 4 parameters parameter NNS cord-104239-xxlcdbqi 190 5 of of IN cord-104239-xxlcdbqi 190 6 the the DT cord-104239-xxlcdbqi 190 7 RBL RBL NNP cord-104239-xxlcdbqi 190 8 and and CC cord-104239-xxlcdbqi 190 9 NRK NRK NNP cord-104239-xxlcdbqi 190 10 cell cell NN cord-104239-xxlcdbqi 190 11 line line NN cord-104239-xxlcdbqi 190 12 used use VBN cord-104239-xxlcdbqi 190 13 in in IN cord-104239-xxlcdbqi 190 14 these these DT cord-104239-xxlcdbqi 190 15 studies study NNS cord-104239-xxlcdbqi 190 16 are be VBP cord-104239-xxlcdbqi 190 17 shown show VBN cord-104239-xxlcdbqi 190 18 in in IN cord-104239-xxlcdbqi 190 19 Table Table NNP cord-104239-xxlcdbqi 190 20 I -PRON- PRP cord-104239-xxlcdbqi 190 21 . . . cord-104239-xxlcdbqi 191 1 To to TO cord-104239-xxlcdbqi 191 2 morphometrically morphometrically RB cord-104239-xxlcdbqi 191 3 evaluate evaluate VB cord-104239-xxlcdbqi 191 4 the the DT cord-104239-xxlcdbqi 191 5 distribution distribution NN cord-104239-xxlcdbqi 191 6 of of IN cord-104239-xxlcdbqi 191 7 export export NN cord-104239-xxlcdbqi 191 8 sites site NNS cord-104239-xxlcdbqi 191 9 , , , cord-104239-xxlcdbqi 191 10 ER er NN cord-104239-xxlcdbqi 191 11 - - HYPH cord-104239-xxlcdbqi 191 12 budding budding JJ cord-104239-xxlcdbqi 191 13 structures structure NNS cord-104239-xxlcdbqi 191 14 were be VBD cord-104239-xxlcdbqi 191 15 identified identify VBN cord-104239-xxlcdbqi 191 16 as as IN cord-104239-xxlcdbqi 191 17 an an DT cord-104239-xxlcdbqi 191 18 elevation elevation NN cord-104239-xxlcdbqi 191 19 on on IN cord-104239-xxlcdbqi 191 20 the the DT cord-104239-xxlcdbqi 191 21 surface surface NN cord-104239-xxlcdbqi 191 22 of of IN cord-104239-xxlcdbqi 191 23 the the DT cord-104239-xxlcdbqi 191 24 ER ER NNP cord-104239-xxlcdbqi 191 25 with with IN cord-104239-xxlcdbqi 191 26 a a DT cord-104239-xxlcdbqi 191 27 width width NN cord-104239-xxlcdbqi 191 28 of of IN cord-104239-xxlcdbqi 191 29 65 65 CD cord-104239-xxlcdbqi 191 30 - - SYM cord-104239-xxlcdbqi 191 31 85 85 CD cord-104239-xxlcdbqi 191 32 nm nm NN cord-104239-xxlcdbqi 191 33 , , , cord-104239-xxlcdbqi 191 34 extruded extrude VBN cord-104239-xxlcdbqi 191 35 from from IN cord-104239-xxlcdbqi 191 36 the the DT cord-104239-xxlcdbqi 191 37 membrane membrane NN cord-104239-xxlcdbqi 191 38 by by IN cord-104239-xxlcdbqi 191 39 at at RB cord-104239-xxlcdbqi 191 40 least least RBS cord-104239-xxlcdbqi 191 41 50 50 CD cord-104239-xxlcdbqi 191 42 % % NN cord-104239-xxlcdbqi 191 43 of of IN cord-104239-xxlcdbqi 191 44 their -PRON- PRP$ cord-104239-xxlcdbqi 191 45 diameter diameter NN cord-104239-xxlcdbqi 191 46 , , , cord-104239-xxlcdbqi 191 47 and and CC cord-104239-xxlcdbqi 191 48 covered cover VBN cord-104239-xxlcdbqi 191 49 with with IN cord-104239-xxlcdbqi 191 50 a a DT cord-104239-xxlcdbqi 191 51 distinctive distinctive JJ cord-104239-xxlcdbqi 191 52 electron electron NN cord-104239-xxlcdbqi 191 53 - - HYPH cord-104239-xxlcdbqi 191 54 dense dense JJ cord-104239-xxlcdbqi 191 55 coat coat NN cord-104239-xxlcdbqi 191 56 ( ( -LRB- cord-104239-xxlcdbqi 191 57 Fig fig NN cord-104239-xxlcdbqi 191 58 . . . cord-104239-xxlcdbqi 191 59 1 1 CD cord-104239-xxlcdbqi 191 60 - - SYM cord-104239-xxlcdbqi 191 61 3 3 CD cord-104239-xxlcdbqi 191 62 , , , cord-104239-xxlcdbqi 191 63 arrowheads arrowhead NNS cord-104239-xxlcdbqi 191 64 ) ) -RRB- cord-104239-xxlcdbqi 191 65 . . . cord-104239-xxlcdbqi 192 1 Budding bud VBG cord-104239-xxlcdbqi 192 2 sites site NNS cord-104239-xxlcdbqi 192 3 emanate emanate VBP cord-104239-xxlcdbqi 192 4 from from IN cord-104239-xxlcdbqi 192 5 three three CD cord-104239-xxlcdbqi 192 6 locations location NNS cord-104239-xxlcdbqi 192 7 in in IN cord-104239-xxlcdbqi 192 8 the the DT cord-104239-xxlcdbqi 192 9 cell cell NN cord-104239-xxlcdbqi 192 10 including include VBG cord-104239-xxlcdbqi 192 11 ( ( -LRB- cord-104239-xxlcdbqi 192 12 a a LS cord-104239-xxlcdbqi 192 13 ) ) -RRB- cord-104239-xxlcdbqi 192 14 those those DT cord-104239-xxlcdbqi 192 15 associated associate VBN cord-104239-xxlcdbqi 192 16 with with IN cord-104239-xxlcdbqi 192 17 the the DT cord-104239-xxlcdbqi 192 18 nuclear nuclear JJ cord-104239-xxlcdbqi 192 19 envelope envelope NN cord-104239-xxlcdbqi 192 20 ( ( -LRB- cord-104239-xxlcdbqi 192 21 Fig Fig NNP cord-104239-xxlcdbqi 192 22 . . . cord-104239-xxlcdbqi 192 23 1 1 CD cord-104239-xxlcdbqi 192 24 ) ) -RRB- cord-104239-xxlcdbqi 192 25 , , , cord-104239-xxlcdbqi 192 26 ( ( -LRB- cord-104239-xxlcdbqi 192 27 b b LS cord-104239-xxlcdbqi 192 28 ) ) -RRB- cord-104239-xxlcdbqi 192 29 those those DT cord-104239-xxlcdbqi 192 30 associated associate VBN cord-104239-xxlcdbqi 192 31 with with IN cord-104239-xxlcdbqi 192 32 the the DT cord-104239-xxlcdbqi 192 33 Golgi Golgi NNP cord-104239-xxlcdbqi 192 34 apparatus apparatus NN cord-104239-xxlcdbqi 192 35 that that WDT cord-104239-xxlcdbqi 192 36 are be VBP cord-104239-xxlcdbqi 192 37 analogous analogous JJ cord-104239-xxlcdbqi 192 38 in in IN cord-104239-xxlcdbqi 192 39 structure structure NN cord-104239-xxlcdbqi 192 40 to to IN cord-104239-xxlcdbqi 192 41 classical classical JJ cord-104239-xxlcdbqi 192 42 ER ER NNP cord-104239-xxlcdbqi 192 43 transitional transitional JJ cord-104239-xxlcdbqi 192 44 elements element NNS cord-104239-xxlcdbqi 192 45 ( ( -LRB- cord-104239-xxlcdbqi 192 46 Palade Palade NNP cord-104239-xxlcdbqi 192 47 , , , cord-104239-xxlcdbqi 192 48 1975 1975 CD cord-104239-xxlcdbqi 192 49 ; ; : cord-104239-xxlcdbqi 192 50 Sesso Sesso NNP cord-104239-xxlcdbqi 192 51 et et FW cord-104239-xxlcdbqi 192 52 al al NNP cord-104239-xxlcdbqi 192 53 . . NNP cord-104239-xxlcdbqi 192 54 , , , cord-104239-xxlcdbqi 192 55 1994 1994 CD cord-104239-xxlcdbqi 192 56 ) ) -RRB- cord-104239-xxlcdbqi 193 1 ( ( -LRB- cord-104239-xxlcdbqi 193 2 Fig Fig NNP cord-104239-xxlcdbqi 193 3 . . NNP cord-104239-xxlcdbqi 193 4 2 2 CD cord-104239-xxlcdbqi 193 5 ) ) -RRB- cord-104239-xxlcdbqi 193 6 , , . cord-104239-xxlcdbqi 194 1 and and CC cord-104239-xxlcdbqi 194 2 ( ( -LRB- cord-104239-xxlcdbqi 194 3 c c NN cord-104239-xxlcdbqi 194 4 ) ) -RRB- cord-104239-xxlcdbqi 194 5 those those DT cord-104239-xxlcdbqi 194 6 found find VBD cord-104239-xxlcdbqi 194 7 in in IN cord-104239-xxlcdbqi 194 8 more more JJR cord-104239-xxlcdbqi 194 9 peripheral peripheral JJ cord-104239-xxlcdbqi 194 10 regions region NNS cord-104239-xxlcdbqi 194 11 that that WDT cord-104239-xxlcdbqi 194 12 lack lack VBP cord-104239-xxlcdbqi 194 13 detectable detectable JJ cord-104239-xxlcdbqi 194 14 Golgi Golgi NNP cord-104239-xxlcdbqi 194 15 ( ( -LRB- cord-104239-xxlcdbqi 194 16 Fig fig NN cord-104239-xxlcdbqi 194 17 . . . cord-104239-xxlcdbqi 194 18 3 3 CD cord-104239-xxlcdbqi 194 19 ) ) -RRB- cord-104239-xxlcdbqi 194 20 . . . cord-104239-xxlcdbqi 195 1 Buds bud NNS cord-104239-xxlcdbqi 195 2 found find VBN cord-104239-xxlcdbqi 195 3 at at IN cord-104239-xxlcdbqi 195 4 the the DT cord-104239-xxlcdbqi 195 5 tip tip NN cord-104239-xxlcdbqi 195 6 of of IN cord-104239-xxlcdbqi 195 7 tubular tubular JJ cord-104239-xxlcdbqi 195 8 projections projection NNS cord-104239-xxlcdbqi 195 9 from from IN cord-104239-xxlcdbqi 195 10 the the DT cord-104239-xxlcdbqi 195 11 surface surface NN cord-104239-xxlcdbqi 195 12 of of IN cord-104239-xxlcdbqi 195 13 the the DT cord-104239-xxlcdbqi 195 14 ER ER NNP cord-104239-xxlcdbqi 195 15 had have VBD cord-104239-xxlcdbqi 195 16 an an DT cord-104239-xxlcdbqi 195 17 average average JJ cord-104239-xxlcdbqi 195 18 diameter diameter NN cord-104239-xxlcdbqi 195 19 of of IN cord-104239-xxlcdbqi 195 20 78 78 CD cord-104239-xxlcdbqi 195 21 ± ± CD cord-104239-xxlcdbqi 195 22 6 6 CD cord-104239-xxlcdbqi 195 23 nm nm NN cord-104239-xxlcdbqi 195 24 . . . cord-104239-xxlcdbqi 196 1 Although although IN cord-104239-xxlcdbqi 196 2 tubular tubular JJ cord-104239-xxlcdbqi 196 3 projections projection NNS cord-104239-xxlcdbqi 196 4 were be VBD cord-104239-xxlcdbqi 196 5 generally generally RB cord-104239-xxlcdbqi 196 6 shorter short JJR cord-104239-xxlcdbqi 196 7 than than IN cord-104239-xxlcdbqi 196 8 150 150 CD cord-104239-xxlcdbqi 196 9 nm nm NN cord-104239-xxlcdbqi 197 1 ( ( -LRB- cord-104239-xxlcdbqi 197 2 Fig Fig NNP cord-104239-xxlcdbqi 197 3 . . NNP cord-104239-xxlcdbqi 197 4 1 1 CD cord-104239-xxlcdbqi 197 5 , , , cord-104239-xxlcdbqi 197 6 section section NN cord-104239-xxlcdbqi 197 7 5 5 CD cord-104239-xxlcdbqi 197 8 ; ; : cord-104239-xxlcdbqi 197 9 arrowheads arrowhead NNS cord-104239-xxlcdbqi 197 10 ) ) -RRB- cord-104239-xxlcdbqi 197 11 , , , cord-104239-xxlcdbqi 197 12 they -PRON- PRP cord-104239-xxlcdbqi 197 13 could could MD cord-104239-xxlcdbqi 197 14 be be VB cord-104239-xxlcdbqi 197 15 as as RB cord-104239-xxlcdbqi 197 16 long long JJ cord-104239-xxlcdbqi 197 17 as as IN cord-104239-xxlcdbqi 197 18 350 350 CD cord-104239-xxlcdbqi 197 19 nm nm NN cord-104239-xxlcdbqi 197 20 based base VBN cord-104239-xxlcdbqi 197 21 on on IN cord-104239-xxlcdbqi 197 22 reconstruction reconstruction NN cord-104239-xxlcdbqi 197 23 from from IN cord-104239-xxlcdbqi 197 24 serial serial JJ cord-104239-xxlcdbqi 197 25 - - HYPH cord-104239-xxlcdbqi 197 26 thin thin JJ cord-104239-xxlcdbqi 197 27 sections section NNS cord-104239-xxlcdbqi 197 28 . . . cord-104239-xxlcdbqi 198 1 Buds bud NNS cord-104239-xxlcdbqi 198 2 were be VBD cord-104239-xxlcdbqi 198 3 covered cover VBN cord-104239-xxlcdbqi 198 4 with with IN cord-104239-xxlcdbqi 198 5 an an DT cord-104239-xxlcdbqi 198 6 ~8 ~8 CD cord-104239-xxlcdbqi 198 7 - - HYPH cord-104239-xxlcdbqi 198 8 10-nm 10-nm CD cord-104239-xxlcdbqi 198 9 - - HYPH cord-104239-xxlcdbqi 198 10 thick thick JJ cord-104239-xxlcdbqi 198 11 electron electron NN cord-104239-xxlcdbqi 198 12 - - HYPH cord-104239-xxlcdbqi 198 13 dense dense JJ cord-104239-xxlcdbqi 198 14 coat coat NN cord-104239-xxlcdbqi 198 15 . . . cord-104239-xxlcdbqi 199 1 On on IN cord-104239-xxlcdbqi 199 2 grazing graze VBG cord-104239-xxlcdbqi 199 3 sections section NNS cord-104239-xxlcdbqi 199 4 and and CC cord-104239-xxlcdbqi 199 5 at at IN cord-104239-xxlcdbqi 199 6 high high JJ cord-104239-xxlcdbqi 199 7 magnification magnification NN cord-104239-xxlcdbqi 199 8 , , , cord-104239-xxlcdbqi 199 9 the the DT cord-104239-xxlcdbqi 199 10 coat coat NN cord-104239-xxlcdbqi 199 11 of of IN cord-104239-xxlcdbqi 199 12 ER ER NNP cord-104239-xxlcdbqi 199 13 buds bud NNS cord-104239-xxlcdbqi 199 14 possessed possess VBD cord-104239-xxlcdbqi 199 15 a a DT cord-104239-xxlcdbqi 199 16 lattice lattice NN cord-104239-xxlcdbqi 199 17 - - HYPH cord-104239-xxlcdbqi 199 18 like like JJ cord-104239-xxlcdbqi 199 19 appearance appearance NN cord-104239-xxlcdbqi 199 20 due due IN cord-104239-xxlcdbqi 199 21 to to IN cord-104239-xxlcdbqi 199 22 a a DT cord-104239-xxlcdbqi 199 23 semi semi JJ cord-104239-xxlcdbqi 199 24 - - JJ cord-104239-xxlcdbqi 199 25 regular regular JJ cord-104239-xxlcdbqi 199 26 array array NN cord-104239-xxlcdbqi 199 27 of of IN cord-104239-xxlcdbqi 199 28 4 4 CD cord-104239-xxlcdbqi 199 29 - - SYM cord-104239-xxlcdbqi 199 30 5-nm 5-nm CD cord-104239-xxlcdbqi 199 31 elongated elongated JJ cord-104239-xxlcdbqi 199 32 particles particle NNS cord-104239-xxlcdbqi 199 33 ( ( -LRB- cord-104239-xxlcdbqi 199 34 Fig Fig NNP cord-104239-xxlcdbqi 199 35 . . NNP cord-104239-xxlcdbqi 199 36 1 1 CD cord-104239-xxlcdbqi 199 37 , , , cord-104239-xxlcdbqi 199 38 inset inset NN cord-104239-xxlcdbqi 199 39 ) ) -RRB- cord-104239-xxlcdbqi 199 40 . . . cord-104239-xxlcdbqi 200 1 These these DT cord-104239-xxlcdbqi 200 2 coats coat NNS cord-104239-xxlcdbqi 200 3 are be VBP cord-104239-xxlcdbqi 200 4 similar similar JJ cord-104239-xxlcdbqi 200 5 to to IN cord-104239-xxlcdbqi 200 6 those those DT cord-104239-xxlcdbqi 200 7 observed observe VBN cord-104239-xxlcdbqi 200 8 in in IN cord-104239-xxlcdbqi 200 9 pancreatic pancreatic JJ cord-104239-xxlcdbqi 200 10 acinar acinar NN cord-104239-xxlcdbqi 200 11 cells cell NNS cord-104239-xxlcdbqi 201 1 ( ( -LRB- cord-104239-xxlcdbqi 201 2 Merisko Merisko NNP cord-104239-xxlcdbqi 201 3 et et NNP cord-104239-xxlcdbqi 201 4 al al NNP cord-104239-xxlcdbqi 201 5 . . NNP cord-104239-xxlcdbqi 201 6 , , , cord-104239-xxlcdbqi 201 7 1986 1986 CD cord-104239-xxlcdbqi 201 8 ) ) -RRB- cord-104239-xxlcdbqi 201 9 . . . cord-104239-xxlcdbqi 202 1 Based base VBN cord-104239-xxlcdbqi 202 2 on on IN cord-104239-xxlcdbqi 202 3 analysis analysis NN cord-104239-xxlcdbqi 202 4 of of IN cord-104239-xxlcdbqi 202 5 random random JJ cord-104239-xxlcdbqi 202 6 sections section NNS cord-104239-xxlcdbqi 202 7 through through IN cord-104239-xxlcdbqi 202 8 ~300 ~300 JJ cord-104239-xxlcdbqi 202 9 cells cell NNS cord-104239-xxlcdbqi 202 10 ( ( -LRB- cord-104239-xxlcdbqi 202 11 see see VB cord-104239-xxlcdbqi 202 12 Materials material NNS cord-104239-xxlcdbqi 202 13 and and CC cord-104239-xxlcdbqi 202 14 Methods Methods NNPS cord-104239-xxlcdbqi 202 15 ) ) -RRB- cord-104239-xxlcdbqi 202 16 , , , cord-104239-xxlcdbqi 202 17 the the DT cord-104239-xxlcdbqi 202 18 average average JJ cord-104239-xxlcdbqi 202 19 number number NN cord-104239-xxlcdbqi 202 20 of of IN cord-104239-xxlcdbqi 202 21 buds bud NNS cord-104239-xxlcdbqi 202 22 in in IN cord-104239-xxlcdbqi 202 23 a a DT cord-104239-xxlcdbqi 202 24 cell cell NN cord-104239-xxlcdbqi 202 25 was be VBD cord-104239-xxlcdbqi 202 26 found find VBN cord-104239-xxlcdbqi 202 27 to to TO cord-104239-xxlcdbqi 202 28 be be VB cord-104239-xxlcdbqi 202 29 250 250 CD cord-104239-xxlcdbqi 202 30 ± ± CD cord-104239-xxlcdbqi 202 31 10.45 10.45 CD cord-104239-xxlcdbqi 202 32 % % NN cord-104239-xxlcdbqi 202 33 of of IN cord-104239-xxlcdbqi 202 34 budding bud VBG cord-104239-xxlcdbqi 202 35 profiles profile NNS cord-104239-xxlcdbqi 202 36 were be VBD cord-104239-xxlcdbqi 202 37 found find VBN cord-104239-xxlcdbqi 202 38 on on IN cord-104239-xxlcdbqi 202 39 ER ER NNP cord-104239-xxlcdbqi 202 40 tubules tubule NNS cord-104239-xxlcdbqi 202 41 located locate VBN cord-104239-xxlcdbqi 202 42 in in IN cord-104239-xxlcdbqi 202 43 the the DT cord-104239-xxlcdbqi 202 44 vicinity vicinity NN cord-104239-xxlcdbqi 202 45 of of IN cord-104239-xxlcdbqi 202 46 Golgi Golgi NNP cord-104239-xxlcdbqi 202 47 complexes complex NNS cord-104239-xxlcdbqi 202 48 , , , cord-104239-xxlcdbqi 202 49 whereas whereas IN cord-104239-xxlcdbqi 202 50 55 55 CD cord-104239-xxlcdbqi 202 51 % % NN cord-104239-xxlcdbqi 202 52 were be VBD cord-104239-xxlcdbqi 202 53 located locate VBN cord-104239-xxlcdbqi 202 54 in in IN cord-104239-xxlcdbqi 202 55 regions region NNS cord-104239-xxlcdbqi 202 56 without without IN cord-104239-xxlcdbqi 202 57 noticeable noticeable JJ cord-104239-xxlcdbqi 202 58 juxtaposition juxtaposition NN cord-104239-xxlcdbqi 202 59 to to IN cord-104239-xxlcdbqi 202 60 Golgi Golgi NNP cord-104239-xxlcdbqi 202 61 . . . cord-104239-xxlcdbqi 203 1 11 11 CD cord-104239-xxlcdbqi 203 2 % % NN cord-104239-xxlcdbqi 203 3 of of IN cord-104239-xxlcdbqi 203 4 total total JJ cord-104239-xxlcdbqi 203 5 buds bud NNS cord-104239-xxlcdbqi 203 6 were be VBD cord-104239-xxlcdbqi 203 7 observed observe VBN cord-104239-xxlcdbqi 203 8 emerging emerge VBG cord-104239-xxlcdbqi 203 9 from from IN cord-104239-xxlcdbqi 203 10 the the DT cord-104239-xxlcdbqi 203 11 nuclear nuclear JJ cord-104239-xxlcdbqi 203 12 envelope envelope NN cord-104239-xxlcdbqi 203 13 . . . cord-104239-xxlcdbqi 204 1 Thus thus RB cord-104239-xxlcdbqi 204 2 , , , cord-104239-xxlcdbqi 204 3 it -PRON- PRP cord-104239-xxlcdbqi 204 4 is be VBZ cord-104239-xxlcdbqi 204 5 apparent apparent JJ cord-104239-xxlcdbqi 204 6 that that IN cord-104239-xxlcdbqi 204 7 a a DT cord-104239-xxlcdbqi 204 8 substantial substantial JJ cord-104239-xxlcdbqi 204 9 level level NN cord-104239-xxlcdbqi 204 10 of of IN cord-104239-xxlcdbqi 204 11 membrane membrane NN cord-104239-xxlcdbqi 204 12 exiting exit VBG cord-104239-xxlcdbqi 204 13 the the DT cord-104239-xxlcdbqi 204 14 ER ER NNP cord-104239-xxlcdbqi 204 15 appears appear VBZ cord-104239-xxlcdbqi 204 16 to to TO cord-104239-xxlcdbqi 204 17 do do VB cord-104239-xxlcdbqi 204 18 so so RB cord-104239-xxlcdbqi 204 19 from from IN cord-104239-xxlcdbqi 204 20 sites site NNS cord-104239-xxlcdbqi 204 21 distant distant JJ cord-104239-xxlcdbqi 204 22 from from IN cord-104239-xxlcdbqi 204 23 Golgi Golgi NNP cord-104239-xxlcdbqi 204 24 elements element NNS cord-104239-xxlcdbqi 204 25 . . . cord-104239-xxlcdbqi 205 1 The the DT cord-104239-xxlcdbqi 205 2 overall overall JJ cord-104239-xxlcdbqi 205 3 average average JJ cord-104239-xxlcdbqi 205 4 density density NN cord-104239-xxlcdbqi 205 5 of of IN cord-104239-xxlcdbqi 205 6 total total JJ cord-104239-xxlcdbqi 205 7 ER ER NNP cord-104239-xxlcdbqi 205 8 buds bud NNS cord-104239-xxlcdbqi 205 9 based base VBN cord-104239-xxlcdbqi 205 10 on on IN cord-104239-xxlcdbqi 205 11 the the DT cord-104239-xxlcdbqi 205 12 cross cross JJ cord-104239-xxlcdbqi 205 13 - - JJ cord-104239-xxlcdbqi 205 14 sectional sectional JJ cord-104239-xxlcdbqi 205 15 volume volume NN cord-104239-xxlcdbqi 205 16 of of IN cord-104239-xxlcdbqi 205 17 the the DT cord-104239-xxlcdbqi 205 18 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 205 19 was be VBD cord-104239-xxlcdbqi 205 20 found find VBN cord-104239-xxlcdbqi 205 21 to to TO cord-104239-xxlcdbqi 205 22 be be VB cord-104239-xxlcdbqi 205 23 0.5 0.5 CD cord-104239-xxlcdbqi 205 24 buds bud NNS cord-104239-xxlcdbqi 205 25 per per IN cord-104239-xxlcdbqi 205 26 pom pom NN cord-104239-xxlcdbqi 205 27 3 3 CD cord-104239-xxlcdbqi 205 28 , , , cord-104239-xxlcdbqi 205 29 or or CC cord-104239-xxlcdbqi 205 30 0.14 0.14 CD cord-104239-xxlcdbqi 205 31 buds bud NNS cord-104239-xxlcdbqi 205 32 per per IN cord-104239-xxlcdbqi 205 33 ixm ixm NN cord-104239-xxlcdbqi 205 34 2 2 CD cord-104239-xxlcdbqi 205 35 of of IN cord-104239-xxlcdbqi 205 36 total total JJ cord-104239-xxlcdbqi 205 37 ER ER NNP cord-104239-xxlcdbqi 205 38 surface surface NN cord-104239-xxlcdbqi 205 39 . . . cord-104239-xxlcdbqi 206 1 However however RB cord-104239-xxlcdbqi 206 2 , , , cord-104239-xxlcdbqi 206 3 the the DT cord-104239-xxlcdbqi 206 4 average average JJ cord-104239-xxlcdbqi 206 5 density density NN cord-104239-xxlcdbqi 206 6 of of IN cord-104239-xxlcdbqi 206 7 ER er NN cord-104239-xxlcdbqi 206 8 - - HYPH cord-104239-xxlcdbqi 206 9 budding budding JJ cord-104239-xxlcdbqi 206 10 profiles profile NNS cord-104239-xxlcdbqi 206 11 found find VBN cord-104239-xxlcdbqi 206 12 within within IN cord-104239-xxlcdbqi 206 13 the the DT cord-104239-xxlcdbqi 206 14 pericentrosomal pericentrosomal JJ cord-104239-xxlcdbqi 206 15 area area NN cord-104239-xxlcdbqi 206 16 containing contain VBG cord-104239-xxlcdbqi 206 17 the the DT cord-104239-xxlcdbqi 206 18 Golgi Golgi NNP cord-104239-xxlcdbqi 206 19 apparatus apparatus NN cord-104239-xxlcdbqi 206 20 ( ( -LRB- cord-104239-xxlcdbqi 206 21 referred refer VBN cord-104239-xxlcdbqi 206 22 to to IN cord-104239-xxlcdbqi 206 23 as as IN cord-104239-xxlcdbqi 206 24 the the DT cord-104239-xxlcdbqi 206 25 Golgi Golgi NNP cord-104239-xxlcdbqi 206 26 exclusion exclusion NN cord-104239-xxlcdbqi 206 27 zone zone NN cord-104239-xxlcdbqi 206 28 ) ) -RRB- cord-104239-xxlcdbqi 206 29 was be VBD cord-104239-xxlcdbqi 206 30 five five CD cord-104239-xxlcdbqi 206 31 - - HYPH cord-104239-xxlcdbqi 206 32 to to IN cord-104239-xxlcdbqi 206 33 sevenfold sevenfold NNP cord-104239-xxlcdbqi 206 34 higher higher RBR cord-104239-xxlcdbqi 206 35 ( ( -LRB- cord-104239-xxlcdbqi 206 36 3.3 3.3 CD cord-104239-xxlcdbqi 206 37 buds bud NNS cord-104239-xxlcdbqi 206 38 per per IN cord-104239-xxlcdbqi 206 39 txm txm NNP cord-104239-xxlcdbqi 206 40 3 3 CD cord-104239-xxlcdbqi 206 41 of of IN cord-104239-xxlcdbqi 206 42 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 206 43 or or CC cord-104239-xxlcdbqi 206 44 0.8 0.8 CD cord-104239-xxlcdbqi 206 45 buds bud NNS cord-104239-xxlcdbqi 206 46 per per IN cord-104239-xxlcdbqi 206 47 ixm ixm NN cord-104239-xxlcdbqi 206 48 2 2 CD cord-104239-xxlcdbqi 206 49 of of IN cord-104239-xxlcdbqi 206 50 ER ER NNP cord-104239-xxlcdbqi 206 51 surface surface NN cord-104239-xxlcdbqi 206 52 ) ) -RRB- cord-104239-xxlcdbqi 206 53 than than IN cord-104239-xxlcdbqi 206 54 the the DT cord-104239-xxlcdbqi 206 55 average average JJ cord-104239-xxlcdbqi 206 56 value value NN cord-104239-xxlcdbqi 206 57 found find VBN cord-104239-xxlcdbqi 206 58 on on IN cord-104239-xxlcdbqi 206 59 the the DT cord-104239-xxlcdbqi 206 60 total total JJ cord-104239-xxlcdbqi 206 61 ER ER NNP cord-104239-xxlcdbqi 206 62 membrane membrane NN cord-104239-xxlcdbqi 206 63 . . . cord-104239-xxlcdbqi 207 1 Outside outside IN cord-104239-xxlcdbqi 207 2 of of IN cord-104239-xxlcdbqi 207 3 the the DT cord-104239-xxlcdbqi 207 4 Golgi Golgi NNP cord-104239-xxlcdbqi 207 5 region region NN cord-104239-xxlcdbqi 207 6 , , , cord-104239-xxlcdbqi 207 7 the the DT cord-104239-xxlcdbqi 207 8 overall overall JJ cord-104239-xxlcdbqi 207 9 mean mean JJ cord-104239-xxlcdbqi 207 10 density density NN cord-104239-xxlcdbqi 207 11 was be VBD cord-104239-xxlcdbqi 207 12 1.5 1.5 CD cord-104239-xxlcdbqi 207 13 - - HYPH cord-104239-xxlcdbqi 207 14 3-fold 3-fold CD cord-104239-xxlcdbqi 207 15 lower low JJR cord-104239-xxlcdbqi 207 16 ( ( -LRB- cord-104239-xxlcdbqi 207 17 0.22 0.22 CD cord-104239-xxlcdbqi 207 18 buds bud NNS cord-104239-xxlcdbqi 207 19 per per IN cord-104239-xxlcdbqi 207 20 ixm ixm NN cord-104239-xxlcdbqi 207 21 3 3 CD cord-104239-xxlcdbqi 207 22 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 207 23 or or CC cord-104239-xxlcdbqi 207 24 0.04 0.04 CD cord-104239-xxlcdbqi 207 25 buds bud NNS cord-104239-xxlcdbqi 207 26 per per IN cord-104239-xxlcdbqi 207 27 ixm ixm NN cord-104239-xxlcdbqi 207 28 2 2 CD cord-104239-xxlcdbqi 207 29 of of IN cord-104239-xxlcdbqi 207 30 ER ER NNP cord-104239-xxlcdbqi 207 31 surface surface NN cord-104239-xxlcdbqi 207 32 ) ) -RRB- cord-104239-xxlcdbqi 207 33 than than IN cord-104239-xxlcdbqi 207 34 the the DT cord-104239-xxlcdbqi 207 35 average average JJ cord-104239-xxlcdbqi 207 36 values value NNS cord-104239-xxlcdbqi 207 37 found find VBN cord-104239-xxlcdbqi 207 38 for for IN cord-104239-xxlcdbqi 207 39 the the DT cord-104239-xxlcdbqi 207 40 cell cell NN cord-104239-xxlcdbqi 207 41 . . . cord-104239-xxlcdbqi 208 1 The the DT cord-104239-xxlcdbqi 208 2 markedly markedly RB cord-104239-xxlcdbqi 208 3 increased increase VBN cord-104239-xxlcdbqi 208 4 budding bud VBG cord-104239-xxlcdbqi 208 5 density density NN cord-104239-xxlcdbqi 208 6 around around IN cord-104239-xxlcdbqi 208 7 the the DT cord-104239-xxlcdbqi 208 8 Golgi Golgi NNP cord-104239-xxlcdbqi 208 9 apparatus apparatus NN cord-104239-xxlcdbqi 208 10 is be VBZ cord-104239-xxlcdbqi 208 11 consistent consistent JJ cord-104239-xxlcdbqi 208 12 with with IN cord-104239-xxlcdbqi 208 13 the the DT cord-104239-xxlcdbqi 208 14 highly highly RB cord-104239-xxlcdbqi 208 15 focused focused JJ cord-104239-xxlcdbqi 208 16 export export NN cord-104239-xxlcdbqi 208 17 activity activity NN cord-104239-xxlcdbqi 208 18 observed observe VBN cord-104239-xxlcdbqi 208 19 from from IN cord-104239-xxlcdbqi 208 20 ER ER NNP cord-104239-xxlcdbqi 208 21 transitional transitional JJ cord-104239-xxlcdbqi 208 22 elements element NNS cord-104239-xxlcdbqi 208 23 present present JJ cord-104239-xxlcdbqi 208 24 in in IN cord-104239-xxlcdbqi 208 25 the the DT cord-104239-xxlcdbqi 208 26 Golgi Golgi NNP cord-104239-xxlcdbqi 208 27 region region NN cord-104239-xxlcdbqi 208 28 of of IN cord-104239-xxlcdbqi 208 29 pancreatic pancreatic JJ cord-104239-xxlcdbqi 208 30 acinar acinar NN cord-104239-xxlcdbqi 208 31 cells cell NNS cord-104239-xxlcdbqi 208 32 ( ( -LRB- cord-104239-xxlcdbqi 208 33 Palade Palade NNP cord-104239-xxlcdbqi 208 34 , , , cord-104239-xxlcdbqi 208 35 1975 1975 CD cord-104239-xxlcdbqi 208 36 ) ) -RRB- cord-104239-xxlcdbqi 208 37 . . . cord-104239-xxlcdbqi 209 1 Figure figure NN cord-104239-xxlcdbqi 209 2 1 1 CD cord-104239-xxlcdbqi 209 3 . . . cord-104239-xxlcdbqi 210 1 Export export NN cord-104239-xxlcdbqi 210 2 sites site NNS cord-104239-xxlcdbqi 210 3 adjacent adjacent JJ cord-104239-xxlcdbqi 210 4 to to IN cord-104239-xxlcdbqi 210 5 the the DT cord-104239-xxlcdbqi 210 6 nuclear nuclear JJ cord-104239-xxlcdbqi 210 7 envelope envelope NN cord-104239-xxlcdbqi 210 8 of of IN cord-104239-xxlcdbqi 210 9 RBL rbl NN cord-104239-xxlcdbqi 210 10 cells cell NNS cord-104239-xxlcdbqi 210 11 . . . cord-104239-xxlcdbqi 211 1 Six six CD cord-104239-xxlcdbqi 211 2 consecutive consecutive JJ cord-104239-xxlcdbqi 211 3 serial serial JJ cord-104239-xxlcdbqi 211 4 sections section NNS cord-104239-xxlcdbqi 211 5 through through IN cord-104239-xxlcdbqi 211 6 a a DT cord-104239-xxlcdbqi 211 7 VTC vtc NN cord-104239-xxlcdbqi 211 8 adjacent adjacent JJ cord-104239-xxlcdbqi 211 9 to to IN cord-104239-xxlcdbqi 211 10 the the DT cord-104239-xxlcdbqi 211 11 nuclear nuclear JJ cord-104239-xxlcdbqi 211 12 envelope envelope NN cord-104239-xxlcdbqi 211 13 . . . cord-104239-xxlcdbqi 212 1 ER ER NNP cord-104239-xxlcdbqi 212 2 buds bud NNS cord-104239-xxlcdbqi 212 3 ( ( -LRB- cord-104239-xxlcdbqi 212 4 arrowheads arrowhead NNS cord-104239-xxlcdbqi 212 5 ) ) -RRB- cord-104239-xxlcdbqi 212 6 emerging emerge VBG cord-104239-xxlcdbqi 212 7 from from IN cord-104239-xxlcdbqi 212 8 the the DT cord-104239-xxlcdbqi 212 9 nuclear nuclear JJ cord-104239-xxlcdbqi 212 10 envelope envelope NN cord-104239-xxlcdbqi 212 11 ( ( -LRB- cord-104239-xxlcdbqi 212 12 section section NN cord-104239-xxlcdbqi 212 13 1 1 CD cord-104239-xxlcdbqi 212 14 ) ) -RRB- cord-104239-xxlcdbqi 212 15 and and CC cord-104239-xxlcdbqi 212 16 parallel parallel JJ cord-104239-xxlcdbqi 212 17 ER ER NNP cord-104239-xxlcdbqi 212 18 membrane membrane NN cord-104239-xxlcdbqi 212 19 ( ( -LRB- cord-104239-xxlcdbqi 212 20 stars star NNS cord-104239-xxlcdbqi 212 21 in in IN cord-104239-xxlcdbqi 212 22 section section NN cord-104239-xxlcdbqi 212 23 2 2 CD cord-104239-xxlcdbqi 212 24 - - : cord-104239-xxlcdbqi 212 25 -6 -6 NN cord-104239-xxlcdbqi 212 26 ) ) -RRB- cord-104239-xxlcdbqi 212 27 are be VBP cord-104239-xxlcdbqi 212 28 facing face VBG cord-104239-xxlcdbqi 212 29 VTCs vtc NNS cord-104239-xxlcdbqi 212 30 . . . cord-104239-xxlcdbqi 213 1 ( ( -LRB- cord-104239-xxlcdbqi 213 2 Inset Inset NNP cord-104239-xxlcdbqi 213 3 ) ) -RRB- cord-104239-xxlcdbqi 213 4 Higher high JJR cord-104239-xxlcdbqi 213 5 magnification magnification NN cord-104239-xxlcdbqi 213 6 view view NN cord-104239-xxlcdbqi 213 7 of of IN cord-104239-xxlcdbqi 213 8 two two CD cord-104239-xxlcdbqi 213 9 ER ER NNP cord-104239-xxlcdbqi 213 10 buds bud NNS cord-104239-xxlcdbqi 213 11 . . . cord-104239-xxlcdbqi 214 1 The the DT cord-104239-xxlcdbqi 214 2 slice slice NN cord-104239-xxlcdbqi 214 3 of of IN cord-104239-xxlcdbqi 214 4 the the DT cord-104239-xxlcdbqi 214 5 section section NN cord-104239-xxlcdbqi 214 6 presented present VBN cord-104239-xxlcdbqi 214 7 in in IN cord-104239-xxlcdbqi 214 8 the the DT cord-104239-xxlcdbqi 214 9 inset inset NN cord-104239-xxlcdbqi 214 10 encompasses encompass VBZ cord-104239-xxlcdbqi 214 11 either either CC cord-104239-xxlcdbqi 214 12 only only RB cord-104239-xxlcdbqi 214 13 the the DT cord-104239-xxlcdbqi 214 14 coat coat NN cord-104239-xxlcdbqi 214 15 ( ( -LRB- cord-104239-xxlcdbqi 214 16 right right RB cord-104239-xxlcdbqi 214 17 ) ) -RRB- cord-104239-xxlcdbqi 214 18 , , , cord-104239-xxlcdbqi 214 19 or or CC cord-104239-xxlcdbqi 214 20 both both CC cord-104239-xxlcdbqi 214 21 the the DT cord-104239-xxlcdbqi 214 22 coat coat NN cord-104239-xxlcdbqi 214 23 and and CC cord-104239-xxlcdbqi 214 24 the the DT cord-104239-xxlcdbqi 214 25 membrane membrane NN cord-104239-xxlcdbqi 214 26 and and CC cord-104239-xxlcdbqi 214 27 the the DT cord-104239-xxlcdbqi 214 28 luminal luminal JJ cord-104239-xxlcdbqi 214 29 part part NN cord-104239-xxlcdbqi 214 30 of of IN cord-104239-xxlcdbqi 214 31 a a DT cord-104239-xxlcdbqi 214 32 bud bud NN cord-104239-xxlcdbqi 214 33 ( ( -LRB- cord-104239-xxlcdbqi 214 34 left left RB cord-104239-xxlcdbqi 214 35 ) ) -RRB- cord-104239-xxlcdbqi 214 36 . . . cord-104239-xxlcdbqi 215 1 Individual individual JJ cord-104239-xxlcdbqi 215 2 4 4 CD cord-104239-xxlcdbqi 215 3 - - SYM cord-104239-xxlcdbqi 215 4 5-nm 5-nm CD cord-104239-xxlcdbqi 215 5 electron electron NN cord-104239-xxlcdbqi 215 6 - - HYPH cord-104239-xxlcdbqi 215 7 dense dense JJ cord-104239-xxlcdbqi 215 8 particles particle NNS cord-104239-xxlcdbqi 215 9 arranged arrange VBN cord-104239-xxlcdbqi 215 10 in in IN cord-104239-xxlcdbqi 215 11 a a DT cord-104239-xxlcdbqi 215 12 semiregular semiregular JJ cord-104239-xxlcdbqi 215 13 pattern pattern NN cord-104239-xxlcdbqi 215 14 ( ( -LRB- cord-104239-xxlcdbqi 215 15 arrows arrow NNS cord-104239-xxlcdbqi 215 16 . . . cord-104239-xxlcdbqi 215 17 ) ) -RRB- cord-104239-xxlcdbqi 216 1 Notice notice VB cord-104239-xxlcdbqi 216 2 the the DT cord-104239-xxlcdbqi 216 3 same same JJ cord-104239-xxlcdbqi 216 4 appearance appearance NN cord-104239-xxlcdbqi 216 5 of of IN cord-104239-xxlcdbqi 216 6 the the DT cord-104239-xxlcdbqi 216 7 coat coat NN cord-104239-xxlcdbqi 216 8 under under IN cord-104239-xxlcdbqi 216 9 lower low JJR cord-104239-xxlcdbqi 216 10 magnification magnification NN cord-104239-xxlcdbqi 216 11 of of IN cord-104239-xxlcdbqi 216 12 serial serial JJ cord-104239-xxlcdbqi 216 13 sections section NNS cord-104239-xxlcdbqi 216 14 that that WDT cord-104239-xxlcdbqi 216 15 contain contain VBP cord-104239-xxlcdbqi 216 16 a a DT cord-104239-xxlcdbqi 216 17 honeycombed honeycombed JJ cord-104239-xxlcdbqi 216 18 appearance appearance NN cord-104239-xxlcdbqi 216 19 consisting consist VBG cord-104239-xxlcdbqi 216 20 of of IN cord-104239-xxlcdbqi 216 21 a a DT cord-104239-xxlcdbqi 216 22 semiregular semiregular JJ cord-104239-xxlcdbqi 216 23 array array NN cord-104239-xxlcdbqi 216 24 of of IN cord-104239-xxlcdbqi 216 25 electron electron NN cord-104239-xxlcdbqi 216 26 - - HYPH cord-104239-xxlcdbqi 216 27 dense dense JJ cord-104239-xxlcdbqi 216 28 particles particle NNS cord-104239-xxlcdbqi 216 29 ( ( -LRB- cord-104239-xxlcdbqi 216 30 arrows arrow NNS cord-104239-xxlcdbqi 216 31 ) ) -RRB- cord-104239-xxlcdbqi 216 32 . . . cord-104239-xxlcdbqi 217 1 Adjacent adjacent JJ cord-104239-xxlcdbqi 217 2 to to IN cord-104239-xxlcdbqi 217 3 buds bud NNS cord-104239-xxlcdbqi 217 4 , , , cord-104239-xxlcdbqi 217 5 we -PRON- PRP cord-104239-xxlcdbqi 217 6 observe observe VBP cord-104239-xxlcdbqi 217 7 a a DT cord-104239-xxlcdbqi 217 8 typical typical JJ cord-104239-xxlcdbqi 217 9 pleomorphic pleomorphic JJ cord-104239-xxlcdbqi 217 10 element element NN cord-104239-xxlcdbqi 217 11 ( ( -LRB- cord-104239-xxlcdbqi 217 12 large large JJ cord-104239-xxlcdbqi 217 13 asterisk asterisk NN cord-104239-xxlcdbqi 217 14 in in IN cord-104239-xxlcdbqi 217 15 section section NN cord-104239-xxlcdbqi 217 16 5 5 CD cord-104239-xxlcdbqi 217 17 ) ) -RRB- cord-104239-xxlcdbqi 217 18 within within IN cord-104239-xxlcdbqi 217 19 a a DT cord-104239-xxlcdbqi 217 20 VTC vtc NN cord-104239-xxlcdbqi 217 21 that that WDT cord-104239-xxlcdbqi 217 22 has have VBZ cord-104239-xxlcdbqi 217 23 numerous numerous JJ cord-104239-xxlcdbqi 217 24 tubular tubular JJ cord-104239-xxlcdbqi 217 25 projections projection NNS cord-104239-xxlcdbqi 217 26 ( ( -LRB- cord-104239-xxlcdbqi 217 27 small small JJ cord-104239-xxlcdbqi 217 28 asterisk asterisk NN cord-104239-xxlcdbqi 217 29 ) ) -RRB- cord-104239-xxlcdbqi 217 30 in in IN cord-104239-xxlcdbqi 217 31 adjacent adjacent JJ cord-104239-xxlcdbqi 217 32 thin thin JJ cord-104239-xxlcdbqi 217 33 - - HYPH cord-104239-xxlcdbqi 217 34 sections section NNS cord-104239-xxlcdbqi 217 35 ( ( -LRB- cord-104239-xxlcdbqi 217 36 small small JJ cord-104239-xxlcdbqi 217 37 asterisks asterisk NNS cord-104239-xxlcdbqi 217 38 in in IN cord-104239-xxlcdbqi 217 39 sections section NNS cord-104239-xxlcdbqi 217 40 4 4 CD cord-104239-xxlcdbqi 217 41 and and CC cord-104239-xxlcdbqi 217 42 6 6 CD cord-104239-xxlcdbqi 217 43 ) ) -RRB- cord-104239-xxlcdbqi 217 44 , , , cord-104239-xxlcdbqi 217 45 indicative indicative JJ cord-104239-xxlcdbqi 217 46 of of IN cord-104239-xxlcdbqi 217 47 its -PRON- PRP$ cord-104239-xxlcdbqi 217 48 fenestrated fenestrated JJ cord-104239-xxlcdbqi 217 49 structure structure NN cord-104239-xxlcdbqi 217 50 . . . cord-104239-xxlcdbqi 218 1 Tubules tubule NNS cord-104239-xxlcdbqi 218 2 in in IN cord-104239-xxlcdbqi 218 3 the the DT cord-104239-xxlcdbqi 218 4 fenestrated fenestrated JJ cord-104239-xxlcdbqi 218 5 elements element NNS cord-104239-xxlcdbqi 218 6 of of IN cord-104239-xxlcdbqi 218 7 the the DT cord-104239-xxlcdbqi 218 8 VTC VTC NNP cord-104239-xxlcdbqi 218 9 possess possess VBP cord-104239-xxlcdbqi 218 10 a a DT cord-104239-xxlcdbqi 218 11 dark dark JJ cord-104239-xxlcdbqi 218 12 dense dense JJ cord-104239-xxlcdbqi 218 13 coat coat NN cord-104239-xxlcdbqi 218 14 ( ( -LRB- cord-104239-xxlcdbqi 218 15 arrows arrow NNS cord-104239-xxlcdbqi 218 16 in in IN cord-104239-xxlcdbqi 218 17 sections section NNS cord-104239-xxlcdbqi 218 18 4 4 CD cord-104239-xxlcdbqi 218 19 and and CC cord-104239-xxlcdbqi 218 20 6 6 CD cord-104239-xxlcdbqi 218 21 ) ) -RRB- cord-104239-xxlcdbqi 218 22 typical typical JJ cord-104239-xxlcdbqi 218 23 of of IN cord-104239-xxlcdbqi 218 24 those those DT cord-104239-xxlcdbqi 218 25 found find VBN cord-104239-xxlcdbqi 218 26 on on IN cord-104239-xxlcdbqi 218 27 Golgi Golgi NNP cord-104239-xxlcdbqi 218 28 compartments compartment NNS cord-104239-xxlcdbqi 218 29 and and CC cord-104239-xxlcdbqi 218 30 are be VBP cord-104239-xxlcdbqi 218 31 readily readily RB cord-104239-xxlcdbqi 218 32 distinguishable distinguishable JJ cord-104239-xxlcdbqi 218 33 from from IN cord-104239-xxlcdbqi 218 34 the the DT cord-104239-xxlcdbqi 218 35 alveolate alveolate NNP cord-104239-xxlcdbqi 218 36 coat coat NN cord-104239-xxlcdbqi 218 37 associated associate VBN cord-104239-xxlcdbqi 218 38 with with IN cord-104239-xxlcdbqi 218 39 COPII COPII NNP cord-104239-xxlcdbqi 218 40 buds bud NNS cord-104239-xxlcdbqi 218 41 emanating emanate VBG cord-104239-xxlcdbqi 218 42 from from IN cord-104239-xxlcdbqi 218 43 the the DT cord-104239-xxlcdbqi 218 44 ER ER NNP cord-104239-xxlcdbqi 218 45 . . . cord-104239-xxlcdbqi 219 1 Bar bar NN cord-104239-xxlcdbqi 219 2 , , , cord-104239-xxlcdbqi 219 3 0.1 0.1 CD cord-104239-xxlcdbqi 219 4 Ixm Ixm NNP cord-104239-xxlcdbqi 219 5 . . . cord-104239-xxlcdbqi 220 1 Figure figure NN cord-104239-xxlcdbqi 220 2 2 2 CD cord-104239-xxlcdbqi 220 3 . . . cord-104239-xxlcdbqi 221 1 Export export NN cord-104239-xxlcdbqi 221 2 complexes complex NNS cord-104239-xxlcdbqi 221 3 adjacent adjacent JJ cord-104239-xxlcdbqi 221 4 to to IN cord-104239-xxlcdbqi 221 5 the the DT cord-104239-xxlcdbqi 221 6 pericentrosomal pericentrosomal JJ cord-104239-xxlcdbqi 221 7 Golgi Golgi NNP cord-104239-xxlcdbqi 221 8 region region NN cord-104239-xxlcdbqi 221 9 of of IN cord-104239-xxlcdbqi 221 10 RBL rbl NN cord-104239-xxlcdbqi 221 11 cells cell NNS cord-104239-xxlcdbqi 221 12 . . . cord-104239-xxlcdbqi 222 1 15 15 CD cord-104239-xxlcdbqi 222 2 consecutive consecutive JJ cord-104239-xxlcdbqi 222 3 serial serial JJ cord-104239-xxlcdbqi 222 4 sections section NNS cord-104239-xxlcdbqi 222 5 through through IN cord-104239-xxlcdbqi 222 6 three three CD cord-104239-xxlcdbqi 222 7 ( ( -LRB- cord-104239-xxlcdbqi 222 8 a a NN cord-104239-xxlcdbqi 222 9 , , , cord-104239-xxlcdbqi 222 10 b b NN cord-104239-xxlcdbqi 222 11 , , , cord-104239-xxlcdbqi 222 12 and and CC cord-104239-xxlcdbqi 222 13 c c LS cord-104239-xxlcdbqi 222 14 ) ) -RRB- cord-104239-xxlcdbqi 222 15 Golgi golgi NN cord-104239-xxlcdbqi 222 16 - - HYPH cord-104239-xxlcdbqi 222 17 adjacent adjacent JJ cord-104239-xxlcdbqi 222 18 export export NN cord-104239-xxlcdbqi 222 19 complexes complex NNS cord-104239-xxlcdbqi 222 20 ( ( -LRB- cord-104239-xxlcdbqi 222 21 encircled encircle VBN cord-104239-xxlcdbqi 222 22 by by IN cord-104239-xxlcdbqi 222 23 dotted dotted JJ cord-104239-xxlcdbqi 222 24 lines line NNS cord-104239-xxlcdbqi 222 25 ) ) -RRB- cord-104239-xxlcdbqi 222 26 are be VBP cord-104239-xxlcdbqi 222 27 shown show VBN cord-104239-xxlcdbqi 222 28 with with IN cord-104239-xxlcdbqi 222 29 ER ER NNP cord-104239-xxlcdbqi 222 30 buds bud NNS cord-104239-xxlcdbqi 222 31 ( ( -LRB- cord-104239-xxlcdbqi 222 32 arrowheads arrowhead NNS cord-104239-xxlcdbqi 222 33 ) ) -RRB- cord-104239-xxlcdbqi 222 34 . . . cord-104239-xxlcdbqi 223 1 Export export NN cord-104239-xxlcdbqi 223 2 complex complex NN cord-104239-xxlcdbqi 223 3 a a DT cord-104239-xxlcdbqi 223 4 contains contain VBZ cord-104239-xxlcdbqi 223 5 13 13 CD cord-104239-xxlcdbqi 223 6 ER er NN cord-104239-xxlcdbqi 223 7 - - HYPH cord-104239-xxlcdbqi 223 8 derived derive VBN cord-104239-xxlcdbqi 223 9 buds bud NNS cord-104239-xxlcdbqi 223 10 , , , cord-104239-xxlcdbqi 223 11 export export NN cord-104239-xxlcdbqi 223 12 complex complex NN cord-104239-xxlcdbqi 223 13 b b NNP cord-104239-xxlcdbqi 223 14 contains contain VBZ cord-104239-xxlcdbqi 223 15 11 11 CD cord-104239-xxlcdbqi 223 16 buds bud NNS cord-104239-xxlcdbqi 223 17 , , , cord-104239-xxlcdbqi 223 18 and and CC cord-104239-xxlcdbqi 223 19 export export NN cord-104239-xxlcdbqi 223 20 complex complex NNP cord-104239-xxlcdbqi 223 21 c c NNP cord-104239-xxlcdbqi 223 22 contains contain VBZ cord-104239-xxlcdbqi 223 23 seven seven CD cord-104239-xxlcdbqi 223 24 buds bud NNS cord-104239-xxlcdbqi 223 25 . . . cord-104239-xxlcdbqi 224 1 Note note VB cord-104239-xxlcdbqi 224 2 the the DT cord-104239-xxlcdbqi 224 3 characteristic characteristic JJ cord-104239-xxlcdbqi 224 4 cup cup NN cord-104239-xxlcdbqi 224 5 - - HYPH cord-104239-xxlcdbqi 224 6 shaped shape VBN cord-104239-xxlcdbqi 224 7 appearance appearance NN cord-104239-xxlcdbqi 224 8 of of IN cord-104239-xxlcdbqi 224 9 ER ER NNP cord-104239-xxlcdbqi 224 10 bud bud NN cord-104239-xxlcdbqi 224 11 - - HYPH cord-104239-xxlcdbqi 224 12 bearing bear VBG cord-104239-xxlcdbqi 224 13 zones zone NNS cord-104239-xxlcdbqi 224 14 especially especially RB cord-104239-xxlcdbqi 224 15 evident evident JJ cord-104239-xxlcdbqi 224 16 for for IN cord-104239-xxlcdbqi 224 17 export export NN cord-104239-xxlcdbqi 224 18 complex complex NNP cord-104239-xxlcdbqi 224 19 b. b. NNP cord-104239-xxlcdbqi 225 1 To to TO cord-104239-xxlcdbqi 225 2 develop develop VB cord-104239-xxlcdbqi 225 3 a a DT cord-104239-xxlcdbqi 225 4 detailed detailed JJ cord-104239-xxlcdbqi 225 5 understanding understanding NN cord-104239-xxlcdbqi 225 6 of of IN cord-104239-xxlcdbqi 225 7 the the DT cord-104239-xxlcdbqi 225 8 topological topological JJ cord-104239-xxlcdbqi 225 9 organization organization NN cord-104239-xxlcdbqi 225 10 of of IN cord-104239-xxlcdbqi 225 11 E E NNP cord-104239-xxlcdbqi 225 12 R r NN cord-104239-xxlcdbqi 225 13 export export NN cord-104239-xxlcdbqi 225 14 throughout throughout IN cord-104239-xxlcdbqi 225 15 the the DT cord-104239-xxlcdbqi 225 16 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 225 17 , , , cord-104239-xxlcdbqi 225 18 we -PRON- PRP cord-104239-xxlcdbqi 225 19 carried carry VBD cord-104239-xxlcdbqi 225 20 out out RP cord-104239-xxlcdbqi 225 21 a a DT cord-104239-xxlcdbqi 225 22 morphological morphological JJ cord-104239-xxlcdbqi 225 23 reconstruction reconstruction NN cord-104239-xxlcdbqi 225 24 of of IN cord-104239-xxlcdbqi 225 25 those those DT cord-104239-xxlcdbqi 225 26 sites site NNS cord-104239-xxlcdbqi 225 27 that that WDT cord-104239-xxlcdbqi 225 28 were be VBD cord-104239-xxlcdbqi 225 29 not not RB cord-104239-xxlcdbqi 225 30 adjacent adjacent JJ cord-104239-xxlcdbqi 225 31 to to IN cord-104239-xxlcdbqi 225 32 Golgi Golgi NNP cord-104239-xxlcdbqi 225 33 elements element NNS cord-104239-xxlcdbqi 225 34 , , , cord-104239-xxlcdbqi 225 35 referred refer VBD cord-104239-xxlcdbqi 225 36 to to IN cord-104239-xxlcdbqi 225 37 as as IN cord-104239-xxlcdbqi 225 38 peripheral peripheral JJ cord-104239-xxlcdbqi 225 39 sites site NNS cord-104239-xxlcdbqi 225 40 . . . cord-104239-xxlcdbqi 226 1 A a DT cord-104239-xxlcdbqi 226 2 n n NN cord-104239-xxlcdbqi 226 3 analysis analysis NN cord-104239-xxlcdbqi 226 4 of of IN cord-104239-xxlcdbqi 226 5 peripheral peripheral JJ cord-104239-xxlcdbqi 226 6 sites site NNS cord-104239-xxlcdbqi 226 7 allows allow VBZ cord-104239-xxlcdbqi 226 8 us -PRON- PRP cord-104239-xxlcdbqi 226 9 to to TO cord-104239-xxlcdbqi 226 10 discriminate discriminate VB cord-104239-xxlcdbqi 226 11 the the DT cord-104239-xxlcdbqi 226 12 structure structure NN cord-104239-xxlcdbqi 226 13 of of IN cord-104239-xxlcdbqi 226 14 ER er NN cord-104239-xxlcdbqi 226 15 - - HYPH cord-104239-xxlcdbqi 226 16 derived derive VBN cord-104239-xxlcdbqi 226 17 intermediates intermediate NNS cord-104239-xxlcdbqi 226 18 from from IN cord-104239-xxlcdbqi 226 19 that that DT cord-104239-xxlcdbqi 226 20 of of IN cord-104239-xxlcdbqi 226 21 the the DT cord-104239-xxlcdbqi 226 22 fenestrated fenestrated JJ cord-104239-xxlcdbqi 226 23 cis cis NNP cord-104239-xxlcdbqi 226 24 - - HYPH cord-104239-xxlcdbqi 226 25 Golgi Golgi NNP cord-104239-xxlcdbqi 226 26 network network NN cord-104239-xxlcdbqi 226 27 ( ( -LRB- cord-104239-xxlcdbqi 226 28 CGN CGN NNP cord-104239-xxlcdbqi 226 29 ) ) -RRB- cord-104239-xxlcdbqi 226 30 , , , cord-104239-xxlcdbqi 226 31 which which WDT cord-104239-xxlcdbqi 226 32 is be VBZ cord-104239-xxlcdbqi 226 33 always always RB cord-104239-xxlcdbqi 226 34 associated associate VBN cord-104239-xxlcdbqi 226 35 with with IN cord-104239-xxlcdbqi 226 36 ER ER NNP cord-104239-xxlcdbqi 226 37 buds bud NNS cord-104239-xxlcdbqi 226 38 near near IN cord-104239-xxlcdbqi 226 39 the the DT cord-104239-xxlcdbqi 226 40 Golgi Golgi NNP cord-104239-xxlcdbqi 226 41 . . . cord-104239-xxlcdbqi 227 1 ER ER NNP cord-104239-xxlcdbqi 227 2 buds bud NNS cord-104239-xxlcdbqi 227 3 on on IN cord-104239-xxlcdbqi 227 4 peripheral peripheral JJ cord-104239-xxlcdbqi 227 5 sites site NNS cord-104239-xxlcdbqi 227 6 ( ( -LRB- cord-104239-xxlcdbqi 227 7 Fig Fig NNP cord-104239-xxlcdbqi 227 8 . . . cord-104239-xxlcdbqi 227 9 3 3 LS cord-104239-xxlcdbqi 227 10 ) ) -RRB- cord-104239-xxlcdbqi 227 11 typically typically RB cord-104239-xxlcdbqi 227 12 emanated emanate VBD cord-104239-xxlcdbqi 227 13 from from IN cord-104239-xxlcdbqi 227 14 short short JJ cord-104239-xxlcdbqi 227 15 stretches stretch NNS cord-104239-xxlcdbqi 227 16 of of IN cord-104239-xxlcdbqi 227 17 ER ER NNP cord-104239-xxlcdbqi 227 18 membrane membrane NN cord-104239-xxlcdbqi 227 19 . . . cord-104239-xxlcdbqi 228 1 These these DT cord-104239-xxlcdbqi 228 2 regions region NNS cord-104239-xxlcdbqi 228 3 were be VBD cord-104239-xxlcdbqi 228 4 separated separate VBN cord-104239-xxlcdbqi 228 5 by by IN cord-104239-xxlcdbqi 228 6 long long JJ cord-104239-xxlcdbqi 228 7 distances distance NNS cord-104239-xxlcdbqi 228 8 from from IN cord-104239-xxlcdbqi 228 9 other other JJ cord-104239-xxlcdbqi 228 10 similar similar JJ cord-104239-xxlcdbqi 228 11 budding budding JJ cord-104239-xxlcdbqi 228 12 foci foci NN cord-104239-xxlcdbqi 228 13 . . . cord-104239-xxlcdbqi 229 1 Frequently frequently RB cord-104239-xxlcdbqi 229 2 , , , cord-104239-xxlcdbqi 229 3 ER ER NNP cord-104239-xxlcdbqi 229 4 buds bud NNS cord-104239-xxlcdbqi 229 5 found find VBN cord-104239-xxlcdbqi 229 6 on on IN cord-104239-xxlcdbqi 229 7 different different JJ cord-104239-xxlcdbqi 229 8 cisternae cisternae NNP cord-104239-xxlcdbqi 229 9 were be VBD cord-104239-xxlcdbqi 229 10 closely closely RB cord-104239-xxlcdbqi 229 11 juxtaposed juxtaposed JJ cord-104239-xxlcdbqi 229 12 and and CC cord-104239-xxlcdbqi 229 13 faced face VBD cord-104239-xxlcdbqi 229 14 each each DT cord-104239-xxlcdbqi 229 15 other other JJ cord-104239-xxlcdbqi 229 16 . . . cord-104239-xxlcdbqi 230 1 These these DT cord-104239-xxlcdbqi 230 2 features feature NNS cord-104239-xxlcdbqi 230 3 are be VBP cord-104239-xxlcdbqi 230 4 more more RBR cord-104239-xxlcdbqi 230 5 evident evident JJ cord-104239-xxlcdbqi 230 6 in in IN cord-104239-xxlcdbqi 230 7 Fig Fig NNP cord-104239-xxlcdbqi 230 8 . . . cord-104239-xxlcdbqi 231 1 4 4 LS cord-104239-xxlcdbqi 231 2 ( ( -LRB- cord-104239-xxlcdbqi 231 3 A a NN cord-104239-xxlcdbqi 231 4 and and CC cord-104239-xxlcdbqi 231 5 B B NNP cord-104239-xxlcdbqi 231 6 ) ) -RRB- cord-104239-xxlcdbqi 231 7 , , , cord-104239-xxlcdbqi 231 8 which which WDT cord-104239-xxlcdbqi 231 9 presents present VBZ cord-104239-xxlcdbqi 231 10 an an DT cord-104239-xxlcdbqi 231 11 overlapping overlap VBG cord-104239-xxlcdbqi 231 12 reconstruction reconstruction NN cord-104239-xxlcdbqi 231 13 of of IN cord-104239-xxlcdbqi 231 14 serial serial JJ cord-104239-xxlcdbqi 231 15 sections section NNS cord-104239-xxlcdbqi 231 16 of of IN cord-104239-xxlcdbqi 231 17 the the DT cord-104239-xxlcdbqi 231 18 peripheral peripheral JJ cord-104239-xxlcdbqi 231 19 site site NN cord-104239-xxlcdbqi 231 20 shown show VBN cord-104239-xxlcdbqi 231 21 in in IN cord-104239-xxlcdbqi 231 22 Fig Fig NNP cord-104239-xxlcdbqi 231 23 . . . cord-104239-xxlcdbqi 232 1 3 3 LS cord-104239-xxlcdbqi 232 2 . . . cord-104239-xxlcdbqi 233 1 Budding bud VBG cord-104239-xxlcdbqi 233 2 profiles profile NNS cord-104239-xxlcdbqi 233 3 ( ( -LRB- cord-104239-xxlcdbqi 233 4 blue blue JJ cord-104239-xxlcdbqi 233 5 ) ) -RRB- cord-104239-xxlcdbqi 233 6 pro pro JJ cord-104239-xxlcdbqi 233 7 - - JJ cord-104239-xxlcdbqi 233 8 trude trude NN cord-104239-xxlcdbqi 233 9 from from IN cord-104239-xxlcdbqi 233 10 the the DT cord-104239-xxlcdbqi 233 11 ER ER NNP cord-104239-xxlcdbqi 233 12 ( ( -LRB- cord-104239-xxlcdbqi 233 13 green green NNP cord-104239-xxlcdbqi 233 14 ) ) -RRB- cord-104239-xxlcdbqi 233 15 into into IN cord-104239-xxlcdbqi 233 16 a a DT cord-104239-xxlcdbqi 233 17 central central JJ cord-104239-xxlcdbqi 233 18 region region NN cord-104239-xxlcdbqi 233 19 containing contain VBG cord-104239-xxlcdbqi 233 20 a a DT cord-104239-xxlcdbqi 233 21 collection collection NN cord-104239-xxlcdbqi 233 22 of of IN cord-104239-xxlcdbqi 233 23 vesicles vesicle NNS cord-104239-xxlcdbqi 233 24 and and CC cord-104239-xxlcdbqi 233 25 tubular tubular JJ cord-104239-xxlcdbqi 233 26 elements element NNS cord-104239-xxlcdbqi 233 27 comprising comprise VBG cord-104239-xxlcdbqi 233 28 VTCs VTCs NNP cord-104239-xxlcdbqi 233 29 ( ( -LRB- cord-104239-xxlcdbqi 233 30 red red NNP cord-104239-xxlcdbqi 233 31 ) ) -RRB- cord-104239-xxlcdbqi 233 32 . . . cord-104239-xxlcdbqi 234 1 This this DT cord-104239-xxlcdbqi 234 2 typical typical JJ cord-104239-xxlcdbqi 234 3 organization organization NN cord-104239-xxlcdbqi 234 4 of of IN cord-104239-xxlcdbqi 234 5 peripheral peripheral JJ cord-104239-xxlcdbqi 234 6 sites site NNS cord-104239-xxlcdbqi 234 7 is be VBZ cord-104239-xxlcdbqi 234 8 also also RB cord-104239-xxlcdbqi 234 9 characteristic characteristic JJ cord-104239-xxlcdbqi 234 10 of of IN cord-104239-xxlcdbqi 234 11 budding bud VBG cord-104239-xxlcdbqi 234 12 sites site NNS cord-104239-xxlcdbqi 234 13 associated associate VBN cord-104239-xxlcdbqi 234 14 with with IN cord-104239-xxlcdbqi 234 15 the the DT cord-104239-xxlcdbqi 234 16 nuclear nuclear JJ cord-104239-xxlcdbqi 234 17 envelope envelope NN cord-104239-xxlcdbqi 234 18 ( ( -LRB- cord-104239-xxlcdbqi 234 19 Fig Fig NNP cord-104239-xxlcdbqi 234 20 . . . cord-104239-xxlcdbqi 234 21 1 1 CD cord-104239-xxlcdbqi 234 22 ) ) -RRB- cord-104239-xxlcdbqi 234 23 and and CC cord-104239-xxlcdbqi 234 24 budding bud VBG cord-104239-xxlcdbqi 234 25 sites site NNS cord-104239-xxlcdbqi 234 26 adjacent adjacent JJ cord-104239-xxlcdbqi 234 27 to to IN cord-104239-xxlcdbqi 234 28 the the DT cord-104239-xxlcdbqi 234 29 Golgi Golgi NNP cord-104239-xxlcdbqi 234 30 stack stack NN cord-104239-xxlcdbqi 235 1 ( ( -LRB- cord-104239-xxlcdbqi 235 2 Fig Fig NNP cord-104239-xxlcdbqi 235 3 . . NNP cord-104239-xxlcdbqi 235 4 2 2 CD cord-104239-xxlcdbqi 235 5 ) ) -RRB- cord-104239-xxlcdbqi 235 6 . . . cord-104239-xxlcdbqi 236 1 The the DT cord-104239-xxlcdbqi 236 2 close close JJ cord-104239-xxlcdbqi 236 3 topological topological JJ cord-104239-xxlcdbqi 236 4 relationship relationship NN cord-104239-xxlcdbqi 236 5 between between IN cord-104239-xxlcdbqi 236 6 ER ER NNP cord-104239-xxlcdbqi 236 7 buds bud NNS cord-104239-xxlcdbqi 236 8 and and CC cord-104239-xxlcdbqi 236 9 distinct distinct JJ cord-104239-xxlcdbqi 236 10 VTCs vtc NNS cord-104239-xxlcdbqi 236 11 suggests suggest VBZ cord-104239-xxlcdbqi 236 12 that that IN cord-104239-xxlcdbqi 236 13 these these DT cord-104239-xxlcdbqi 236 14 structures structure NNS cord-104239-xxlcdbqi 236 15 function function VBP cord-104239-xxlcdbqi 236 16 as as IN cord-104239-xxlcdbqi 236 17 a a DT cord-104239-xxlcdbqi 236 18 compact compact JJ cord-104239-xxlcdbqi 236 19 morphological morphological JJ cord-104239-xxlcdbqi 236 20 unit unit NN cord-104239-xxlcdbqi 236 21 that that WDT cord-104239-xxlcdbqi 236 22 we -PRON- PRP cord-104239-xxlcdbqi 236 23 now now RB cord-104239-xxlcdbqi 236 24 refer refer VBP cord-104239-xxlcdbqi 236 25 to to IN cord-104239-xxlcdbqi 236 26 in in IN cord-104239-xxlcdbqi 236 27 its -PRON- PRP$ cord-104239-xxlcdbqi 236 28 entirety entirety NN cord-104239-xxlcdbqi 236 29 as as IN cord-104239-xxlcdbqi 236 30 an an DT cord-104239-xxlcdbqi 236 31 export export NN cord-104239-xxlcdbqi 236 32 complex complex NN cord-104239-xxlcdbqi 236 33 . . . cord-104239-xxlcdbqi 237 1 Morphometric morphometric JJ cord-104239-xxlcdbqi 237 2 analysis analysis NN cord-104239-xxlcdbqi 237 3 of of IN cord-104239-xxlcdbqi 237 4 peripheral peripheral JJ cord-104239-xxlcdbqi 237 5 export export NN cord-104239-xxlcdbqi 237 6 complexes complex NNS cord-104239-xxlcdbqi 237 7 revealed reveal VBD cord-104239-xxlcdbqi 237 8 that that IN cord-104239-xxlcdbqi 237 9 these these DT cord-104239-xxlcdbqi 237 10 sites site NNS cord-104239-xxlcdbqi 237 11 typically typically RB cord-104239-xxlcdbqi 237 12 contained contain VBD cord-104239-xxlcdbqi 237 13 two two CD cord-104239-xxlcdbqi 237 14 to to TO cord-104239-xxlcdbqi 237 15 six six CD cord-104239-xxlcdbqi 237 16 buds bud NNS cord-104239-xxlcdbqi 237 17 emanating emanate VBG cord-104239-xxlcdbqi 237 18 from from IN cord-104239-xxlcdbqi 237 19 the the DT cord-104239-xxlcdbqi 237 20 ER ER NNP cord-104239-xxlcdbqi 237 21 , , , cord-104239-xxlcdbqi 237 22 although although IN cord-104239-xxlcdbqi 237 23 this this DT cord-104239-xxlcdbqi 237 24 could could MD cord-104239-xxlcdbqi 237 25 approach approach VB cord-104239-xxlcdbqi 237 26 a a DT cord-104239-xxlcdbqi 237 27 value value NN cord-104239-xxlcdbqi 237 28 of of IN cord-104239-xxlcdbqi 237 29 20 20 CD cord-104239-xxlcdbqi 237 30 for for IN cord-104239-xxlcdbqi 237 31 some some DT cord-104239-xxlcdbqi 237 32 exceptional exceptional JJ cord-104239-xxlcdbqi 237 33 , , , cord-104239-xxlcdbqi 237 34 larger large JJR cord-104239-xxlcdbqi 237 35 clusters cluster NNS cord-104239-xxlcdbqi 237 36 ( ( -LRB- cord-104239-xxlcdbqi 237 37 Fig fig NN cord-104239-xxlcdbqi 237 38 . . . cord-104239-xxlcdbqi 237 39 5 5 CD cord-104239-xxlcdbqi 237 40 A a NN cord-104239-xxlcdbqi 237 41 , , , cord-104239-xxlcdbqi 237 42 closed closed JJ cord-104239-xxlcdbqi 237 43 circles circle NNS cord-104239-xxlcdbqi 237 44 ) ) -RRB- cord-104239-xxlcdbqi 237 45 . . . cord-104239-xxlcdbqi 238 1 These these DT cord-104239-xxlcdbqi 238 2 sites site NNS cord-104239-xxlcdbqi 238 3 had have VBD cord-104239-xxlcdbqi 238 4 an an DT cord-104239-xxlcdbqi 238 5 average average JJ cord-104239-xxlcdbqi 238 6 number number NN cord-104239-xxlcdbqi 238 7 of of IN cord-104239-xxlcdbqi 238 8 buds bud NNS cord-104239-xxlcdbqi 238 9 per per IN cord-104239-xxlcdbqi 238 10 site site NN cord-104239-xxlcdbqi 238 11 of of IN cord-104239-xxlcdbqi 238 12 4.4 4.4 CD cord-104239-xxlcdbqi 239 1 _ _ NNP cord-104239-xxlcdbqi 239 2 _ _ NNP cord-104239-xxlcdbqi 239 3 _ _ NNP cord-104239-xxlcdbqi 239 4 0.3 0.3 CD cord-104239-xxlcdbqi 239 5 ( ( -LRB- cord-104239-xxlcdbqi 239 6 Table Table NNP cord-104239-xxlcdbqi 239 7 II II NNP cord-104239-xxlcdbqi 239 8 ) ) -RRB- cord-104239-xxlcdbqi 239 9 , , , cord-104239-xxlcdbqi 239 10 a a DT cord-104239-xxlcdbqi 239 11 value value NN cord-104239-xxlcdbqi 239 12 that that WDT cord-104239-xxlcdbqi 239 13 was be VBD cord-104239-xxlcdbqi 239 14 slightly slightly RB cord-104239-xxlcdbqi 239 15 smaller small JJR cord-104239-xxlcdbqi 239 16 than than IN cord-104239-xxlcdbqi 239 17 the the DT cord-104239-xxlcdbqi 239 18 average average JJ cord-104239-xxlcdbqi 239 19 number number NN cord-104239-xxlcdbqi 239 20 of of IN cord-104239-xxlcdbqi 239 21 buds bud NNS cord-104239-xxlcdbqi 239 22 per per IN cord-104239-xxlcdbqi 239 23 site site NN cord-104239-xxlcdbqi 239 24 found find VBN cord-104239-xxlcdbqi 239 25 in in IN cord-104239-xxlcdbqi 239 26 export export NN cord-104239-xxlcdbqi 239 27 complexes complex NNS cord-104239-xxlcdbqi 239 28 adjacent adjacent JJ cord-104239-xxlcdbqi 239 29 to to IN cord-104239-xxlcdbqi 239 30 Golgi Golgi NNP cord-104239-xxlcdbqi 239 31 ( ( -LRB- cord-104239-xxlcdbqi 239 32 6.1 6.1 CD cord-104239-xxlcdbqi 239 33 + + CC cord-104239-xxlcdbqi 239 34 _ _ NN cord-104239-xxlcdbqi 239 35 0.4 0.4 CD cord-104239-xxlcdbqi 239 36 buds bud NNS cord-104239-xxlcdbqi 239 37 per per IN cord-104239-xxlcdbqi 239 38 site site NN cord-104239-xxlcdbqi 239 39 ; ; : cord-104239-xxlcdbqi 239 40 Table Table NNP cord-104239-xxlcdbqi 239 41 II II NNP cord-104239-xxlcdbqi 239 42 ) ) -RRB- cord-104239-xxlcdbqi 239 43 . . . cord-104239-xxlcdbqi 240 1 By by IN cord-104239-xxlcdbqi 240 2 including include VBG cord-104239-xxlcdbqi 240 3 only only RB cord-104239-xxlcdbqi 240 4 ER er NN cord-104239-xxlcdbqi 240 5 - - HYPH cord-104239-xxlcdbqi 240 6 budding budding JJ cord-104239-xxlcdbqi 240 7 profiles profile NNS cord-104239-xxlcdbqi 240 8 facing face VBG cord-104239-xxlcdbqi 240 9 VTCs vtc NNS cord-104239-xxlcdbqi 240 10 , , , cord-104239-xxlcdbqi 240 11 we -PRON- PRP cord-104239-xxlcdbqi 240 12 were be VBD cord-104239-xxlcdbqi 240 13 able able JJ cord-104239-xxlcdbqi 240 14 to to TO cord-104239-xxlcdbqi 240 15 estimate estimate VB cord-104239-xxlcdbqi 240 16 a a DT cord-104239-xxlcdbqi 240 17 " " `` cord-104239-xxlcdbqi 240 18 local local JJ cord-104239-xxlcdbqi 240 19 " " '' cord-104239-xxlcdbqi 240 20 bud bud NN cord-104239-xxlcdbqi 240 21 density density NN cord-104239-xxlcdbqi 240 22 in in IN cord-104239-xxlcdbqi 240 23 these these DT cord-104239-xxlcdbqi 240 24 export export NN cord-104239-xxlcdbqi 240 25 complexes complex NNS cord-104239-xxlcdbqi 240 26 . . . cord-104239-xxlcdbqi 241 1 On on IN cord-104239-xxlcdbqi 241 2 average average JJ cord-104239-xxlcdbqi 241 3 , , , cord-104239-xxlcdbqi 241 4 this this DT cord-104239-xxlcdbqi 241 5 value value NN cord-104239-xxlcdbqi 241 6 was be VBD cord-104239-xxlcdbqi 241 7 17.2 17.2 CD cord-104239-xxlcdbqi 241 8 + + CC cord-104239-xxlcdbqi 241 9 _ _ NN cord-104239-xxlcdbqi 241 10 _ _ NN cord-104239-xxlcdbqi 241 11 2.5 2.5 CD cord-104239-xxlcdbqi 241 12 buds bud NNS cord-104239-xxlcdbqi 241 13 per per IN cord-104239-xxlcdbqi 241 14 i i NN cord-104239-xxlcdbqi 241 15 ~ ~ CD cord-104239-xxlcdbqi 241 16 m m NN cord-104239-xxlcdbqi 241 17 2 2 CD cord-104239-xxlcdbqi 241 18 of of IN cord-104239-xxlcdbqi 241 19 ER ER NNP cord-104239-xxlcdbqi 241 20 surface surface NN cord-104239-xxlcdbqi 241 21 , , , cord-104239-xxlcdbqi 241 22 which which WDT cord-104239-xxlcdbqi 241 23 , , , cord-104239-xxlcdbqi 241 24 on on IN cord-104239-xxlcdbqi 241 25 a a DT cord-104239-xxlcdbqi 241 26 relative relative JJ cord-104239-xxlcdbqi 241 27 scale scale NN cord-104239-xxlcdbqi 241 28 , , , cord-104239-xxlcdbqi 241 29 is be VBZ cord-104239-xxlcdbqi 241 30 125 125 CD cord-104239-xxlcdbqi 241 31 times time NNS cord-104239-xxlcdbqi 241 32 higher high JJR cord-104239-xxlcdbqi 241 33 than than IN cord-104239-xxlcdbqi 241 34 the the DT cord-104239-xxlcdbqi 241 35 average average JJ cord-104239-xxlcdbqi 241 36 bud bud NN cord-104239-xxlcdbqi 241 37 density density NN cord-104239-xxlcdbqi 241 38 found find VBN cord-104239-xxlcdbqi 241 39 on on IN cord-104239-xxlcdbqi 241 40 the the DT cord-104239-xxlcdbqi 241 41 total total JJ cord-104239-xxlcdbqi 241 42 ER er NN cord-104239-xxlcdbqi 241 43 surface surface NN cord-104239-xxlcdbqi 241 44 ( ( -LRB- cord-104239-xxlcdbqi 241 45 0.14 0.14 CD cord-104239-xxlcdbqi 241 46 buds bud NNS cord-104239-xxlcdbqi 241 47 per per IN cord-104239-xxlcdbqi 241 48 ixm ixm NN cord-104239-xxlcdbqi 241 49 2 2 CD cord-104239-xxlcdbqi 241 50 ) ) -RRB- cord-104239-xxlcdbqi 241 51 and and CC cord-104239-xxlcdbqi 241 52 approximately approximately RB cord-104239-xxlcdbqi 241 53 five five CD cord-104239-xxlcdbqi 241 54 times time NNS cord-104239-xxlcdbqi 241 55 higher high JJR cord-104239-xxlcdbqi 241 56 than than IN cord-104239-xxlcdbqi 241 57 the the DT cord-104239-xxlcdbqi 241 58 bud bud NN cord-104239-xxlcdbqi 241 59 density density NN cord-104239-xxlcdbqi 241 60 found find VBN cord-104239-xxlcdbqi 241 61 on on IN cord-104239-xxlcdbqi 241 62 the the DT cord-104239-xxlcdbqi 241 63 surface surface NN cord-104239-xxlcdbqi 241 64 of of IN cord-104239-xxlcdbqi 241 65 the the DT cord-104239-xxlcdbqi 241 66 ER ER NNP cord-104239-xxlcdbqi 241 67 within within IN cord-104239-xxlcdbqi 241 68 the the DT cord-104239-xxlcdbqi 241 69 Golgi Golgi NNP cord-104239-xxlcdbqi 241 70 exclusion exclusion NN cord-104239-xxlcdbqi 241 71 zone zone NN cord-104239-xxlcdbqi 241 72 . . . cord-104239-xxlcdbqi 242 1 The the DT cord-104239-xxlcdbqi 242 2 apparent apparent JJ cord-104239-xxlcdbqi 242 3 nonrandom nonrandom JJ cord-104239-xxlcdbqi 242 4 distribution distribution NN cord-104239-xxlcdbqi 242 5 of of IN cord-104239-xxlcdbqi 242 6 ER ER NNP cord-104239-xxlcdbqi 242 7 buds bud NNS cord-104239-xxlcdbqi 242 8 led lead VBD cord-104239-xxlcdbqi 242 9 us -PRON- PRP cord-104239-xxlcdbqi 242 10 to to TO cord-104239-xxlcdbqi 242 11 quantitatively quantitatively RB cord-104239-xxlcdbqi 242 12 estimate estimate VB cord-104239-xxlcdbqi 242 13 the the DT cord-104239-xxlcdbqi 242 14 probability probability NN cord-104239-xxlcdbqi 242 15 of of IN cord-104239-xxlcdbqi 242 16 a a DT cord-104239-xxlcdbqi 242 17 given give VBN cord-104239-xxlcdbqi 242 18 bud bud NN cord-104239-xxlcdbqi 242 19 having have VBG cord-104239-xxlcdbqi 242 20 proximity proximity NN cord-104239-xxlcdbqi 242 21 to to IN cord-104239-xxlcdbqi 242 22 a a DT cord-104239-xxlcdbqi 242 23 second second JJ cord-104239-xxlcdbqi 242 24 bud bud NN cord-104239-xxlcdbqi 242 25 in in IN cord-104239-xxlcdbqi 242 26 the the DT cord-104239-xxlcdbqi 242 27 cell cell NN cord-104239-xxlcdbqi 242 28 . . . cord-104239-xxlcdbqi 243 1 For for IN cord-104239-xxlcdbqi 243 2 this this DT cord-104239-xxlcdbqi 243 3 purpose purpose NN cord-104239-xxlcdbqi 243 4 , , , cord-104239-xxlcdbqi 243 5 we -PRON- PRP cord-104239-xxlcdbqi 243 6 constructed construct VBD cord-104239-xxlcdbqi 243 7 a a DT cord-104239-xxlcdbqi 243 8 series series NN cord-104239-xxlcdbqi 243 9 of of IN cord-104239-xxlcdbqi 243 10 concentric concentric JJ cord-104239-xxlcdbqi 243 11 shells shell NNS cord-104239-xxlcdbqi 243 12 of of IN cord-104239-xxlcdbqi 243 13 equal equal JJ cord-104239-xxlcdbqi 243 14 volume volume NN cord-104239-xxlcdbqi 243 15 that that WDT cord-104239-xxlcdbqi 243 16 radiate radiate VBP cord-104239-xxlcdbqi 243 17 outward outward RB cord-104239-xxlcdbqi 243 18 from from IN cord-104239-xxlcdbqi 243 19 the the DT cord-104239-xxlcdbqi 243 20 center center NN cord-104239-xxlcdbqi 243 21 of of IN cord-104239-xxlcdbqi 243 22 randomly randomly RB cord-104239-xxlcdbqi 243 23 chosen choose VBN cord-104239-xxlcdbqi 243 24 buds bud NNS cord-104239-xxlcdbqi 243 25 . . . cord-104239-xxlcdbqi 244 1 The the DT cord-104239-xxlcdbqi 244 2 radius radius NN cord-104239-xxlcdbqi 244 3 of of IN cord-104239-xxlcdbqi 244 4 the the DT cord-104239-xxlcdbqi 244 5 first first JJ cord-104239-xxlcdbqi 244 6 internal internal JJ cord-104239-xxlcdbqi 244 7 shell shell NN cord-104239-xxlcdbqi 244 8 was be VBD cord-104239-xxlcdbqi 244 9 arbitrarily arbitrarily RB cord-104239-xxlcdbqi 244 10 assigned assign VBN cord-104239-xxlcdbqi 244 11 a a DT cord-104239-xxlcdbqi 244 12 value value NN cord-104239-xxlcdbqi 244 13 of of IN cord-104239-xxlcdbqi 244 14 0.1 0.1 CD cord-104239-xxlcdbqi 244 15 txm txm NN cord-104239-xxlcdbqi 244 16 to to TO cord-104239-xxlcdbqi 244 17 encompass encompass VB cord-104239-xxlcdbqi 244 18 the the DT cord-104239-xxlcdbqi 244 19 entire entire JJ cord-104239-xxlcdbqi 244 20 tip tip NN cord-104239-xxlcdbqi 244 21 of of IN cord-104239-xxlcdbqi 244 22 a a DT cord-104239-xxlcdbqi 244 23 budding budding JJ cord-104239-xxlcdbqi 244 24 structure structure NN cord-104239-xxlcdbqi 244 25 . . . cord-104239-xxlcdbqi 245 1 Each each DT cord-104239-xxlcdbqi 245 2 increment increment NN cord-104239-xxlcdbqi 245 3 in in IN cord-104239-xxlcdbqi 245 4 the the DT cord-104239-xxlcdbqi 245 5 diameter diameter NN cord-104239-xxlcdbqi 245 6 of of IN cord-104239-xxlcdbqi 245 7 successive successive JJ cord-104239-xxlcdbqi 245 8 shells shell NNS cord-104239-xxlcdbqi 245 9 extending extend VBG cord-104239-xxlcdbqi 245 10 outward outward RB cord-104239-xxlcdbqi 245 11 from from IN cord-104239-xxlcdbqi 245 12 the the DT cord-104239-xxlcdbqi 245 13 first first JJ cord-104239-xxlcdbqi 245 14 shell shell NN cord-104239-xxlcdbqi 245 15 progressively progressively RB cord-104239-xxlcdbqi 245 16 decreased decrease VBD cord-104239-xxlcdbqi 245 17 in in IN cord-104239-xxlcdbqi 245 18 dimension dimension NN cord-104239-xxlcdbqi 245 19 to to TO cord-104239-xxlcdbqi 245 20 encompass encompass VB cord-104239-xxlcdbqi 245 21 the the DT cord-104239-xxlcdbqi 245 22 same same JJ cord-104239-xxlcdbqi 245 23 volume volume NN cord-104239-xxlcdbqi 245 24 in in IN cord-104239-xxlcdbqi 245 25 three three CD cord-104239-xxlcdbqi 245 26 - - HYPH cord-104239-xxlcdbqi 245 27 dimensional dimensional JJ cord-104239-xxlcdbqi 245 28 space space NN cord-104239-xxlcdbqi 245 29 . . . cord-104239-xxlcdbqi 246 1 Given give VBN cord-104239-xxlcdbqi 246 2 the the DT cord-104239-xxlcdbqi 246 3 average average JJ cord-104239-xxlcdbqi 246 4 number number NN cord-104239-xxlcdbqi 246 5 of of IN cord-104239-xxlcdbqi 246 6 buds bud NNS cord-104239-xxlcdbqi 246 7 in in IN cord-104239-xxlcdbqi 246 8 a a DT cord-104239-xxlcdbqi 246 9 cell cell NN cord-104239-xxlcdbqi 246 10 ( ( -LRB- cord-104239-xxlcdbqi 246 11 ~250 ~250 NNP cord-104239-xxlcdbqi 246 12 ) ) -RRB- cord-104239-xxlcdbqi 246 13 , , , cord-104239-xxlcdbqi 246 14 the the DT cord-104239-xxlcdbqi 246 15 volume volume NN cord-104239-xxlcdbqi 246 16 of of IN cord-104239-xxlcdbqi 246 17 cell cell NN cord-104239-xxlcdbqi 246 18 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 246 19 ( ( -LRB- cord-104239-xxlcdbqi 246 20 560 560 CD cord-104239-xxlcdbqi 246 21 i i NN cord-104239-xxlcdbqi 246 22 ~ ~ CD cord-104239-xxlcdbqi 246 23 m3 m3 NNP cord-104239-xxlcdbqi 246 24 ) ) -RRB- cord-104239-xxlcdbqi 246 25 , , , cord-104239-xxlcdbqi 246 26 and and CC cord-104239-xxlcdbqi 246 27 the the DT cord-104239-xxlcdbqi 246 28 volume volume NN cord-104239-xxlcdbqi 246 29 of of IN cord-104239-xxlcdbqi 246 30 a a DT cord-104239-xxlcdbqi 246 31 shell shell NN cord-104239-xxlcdbqi 246 32 ( ( -LRB- cord-104239-xxlcdbqi 246 33 4.2 4.2 CD cord-104239-xxlcdbqi 246 34 × × CD cord-104239-xxlcdbqi 246 35 10 10 CD cord-104239-xxlcdbqi 246 36 .3 .3 . cord-104239-xxlcdbqi 246 37 i i NN cord-104239-xxlcdbqi 246 38 ~ ~ CD cord-104239-xxlcdbqi 246 39 m3 m3 NNP cord-104239-xxlcdbqi 246 40 ) ) -RRB- cord-104239-xxlcdbqi 246 41 , , , cord-104239-xxlcdbqi 246 42 if if IN cord-104239-xxlcdbqi 246 43 buds bud NNS cord-104239-xxlcdbqi 246 44 assumed assume VBD cord-104239-xxlcdbqi 246 45 a a DT cord-104239-xxlcdbqi 246 46 strictly strictly RB cord-104239-xxlcdbqi 246 47 random random JJ cord-104239-xxlcdbqi 246 48 distribution distribution NN cord-104239-xxlcdbqi 246 49 , , , cord-104239-xxlcdbqi 246 50 then then RB cord-104239-xxlcdbqi 246 51 the the DT cord-104239-xxlcdbqi 246 52 probability probability NN cord-104239-xxlcdbqi 246 53 of of IN cord-104239-xxlcdbqi 246 54 encountering encounter VBG cord-104239-xxlcdbqi 246 55 another another DT cord-104239-xxlcdbqi 246 56 bud bud NN cord-104239-xxlcdbqi 246 57 in in IN cord-104239-xxlcdbqi 246 58 a a DT cord-104239-xxlcdbqi 246 59 given give VBN cord-104239-xxlcdbqi 246 60 concentric concentric JJ cord-104239-xxlcdbqi 246 61 shell shell NN cord-104239-xxlcdbqi 246 62 would would MD cord-104239-xxlcdbqi 246 63 remain remain VB cord-104239-xxlcdbqi 246 64 equal equal JJ cord-104239-xxlcdbqi 246 65 with with IN cord-104239-xxlcdbqi 246 66 a a DT cord-104239-xxlcdbqi 246 67 value value NN cord-104239-xxlcdbqi 246 68 of of IN cord-104239-xxlcdbqi 246 69 0.18 0.18 CD cord-104239-xxlcdbqi 246 70 % % NN cord-104239-xxlcdbqi 246 71 ( ( -LRB- cord-104239-xxlcdbqi 246 72 Fig Fig NNP cord-104239-xxlcdbqi 246 73 . . NNP cord-104239-xxlcdbqi 246 74 6 6 CD cord-104239-xxlcdbqi 246 75 , , , cord-104239-xxlcdbqi 246 76 diamonds diamond NNS cord-104239-xxlcdbqi 246 77 ) ) -RRB- cord-104239-xxlcdbqi 246 78 . . . cord-104239-xxlcdbqi 247 1 However however RB cord-104239-xxlcdbqi 247 2 , , , cord-104239-xxlcdbqi 247 3 if if IN cord-104239-xxlcdbqi 247 4 buds bud NNS cord-104239-xxlcdbqi 247 5 were be VBD cord-104239-xxlcdbqi 247 6 confined confine VBN cord-104239-xxlcdbqi 247 7 to to IN cord-104239-xxlcdbqi 247 8 regional regional JJ cord-104239-xxlcdbqi 247 9 foci foci NN cord-104239-xxlcdbqi 247 10 , , , cord-104239-xxlcdbqi 247 11 the the DT cord-104239-xxlcdbqi 247 12 probability probability NN cord-104239-xxlcdbqi 247 13 of of IN cord-104239-xxlcdbqi 247 14 encountering encounter VBG cord-104239-xxlcdbqi 247 15 a a DT cord-104239-xxlcdbqi 247 16 second second JJ cord-104239-xxlcdbqi 247 17 bud bud NN cord-104239-xxlcdbqi 247 18 would would MD cord-104239-xxlcdbqi 247 19 be be VB cord-104239-xxlcdbqi 247 20 high high JJ cord-104239-xxlcdbqi 247 21 in in IN cord-104239-xxlcdbqi 247 22 the the DT cord-104239-xxlcdbqi 247 23 first first JJ cord-104239-xxlcdbqi 247 24 series series NN cord-104239-xxlcdbqi 247 25 of of IN cord-104239-xxlcdbqi 247 26 concentric concentric JJ cord-104239-xxlcdbqi 247 27 shells shell NNS cord-104239-xxlcdbqi 247 28 , , , cord-104239-xxlcdbqi 247 29 and and CC cord-104239-xxlcdbqi 247 30 then then RB cord-104239-xxlcdbqi 247 31 fall fall VB cord-104239-xxlcdbqi 247 32 off off RP cord-104239-xxlcdbqi 247 33 very very RB cord-104239-xxlcdbqi 247 34 rapidly rapidly RB cord-104239-xxlcdbqi 247 35 with with IN cord-104239-xxlcdbqi 247 36 increasing increase VBG cord-104239-xxlcdbqi 247 37 distance distance NN cord-104239-xxlcdbqi 247 38 in in IN cord-104239-xxlcdbqi 247 39 threedimensional threedimensional JJ cord-104239-xxlcdbqi 247 40 space space NN cord-104239-xxlcdbqi 247 41 . . . cord-104239-xxlcdbqi 248 1 The the DT cord-104239-xxlcdbqi 248 2 results result NNS cord-104239-xxlcdbqi 248 3 of of IN cord-104239-xxlcdbqi 248 4 such such PDT cord-104239-xxlcdbqi 248 5 an an DT cord-104239-xxlcdbqi 248 6 analysis analysis NN cord-104239-xxlcdbqi 248 7 are be VBP cord-104239-xxlcdbqi 248 8 shown show VBN cord-104239-xxlcdbqi 248 9 in in IN cord-104239-xxlcdbqi 248 10 Fig Fig NNP cord-104239-xxlcdbqi 248 11 . . . cord-104239-xxlcdbqi 249 1 6 6 CD cord-104239-xxlcdbqi 250 1 where where WRB cord-104239-xxlcdbqi 250 2 we -PRON- PRP cord-104239-xxlcdbqi 250 3 have have VBP cord-104239-xxlcdbqi 250 4 plotted plot VBN cord-104239-xxlcdbqi 250 5 the the DT cord-104239-xxlcdbqi 250 6 probability probability NN cord-104239-xxlcdbqi 250 7 of of IN cord-104239-xxlcdbqi 250 8 encountering encounter VBG cord-104239-xxlcdbqi 250 9 a a DT cord-104239-xxlcdbqi 250 10 bud bud NN cord-104239-xxlcdbqi 250 11 relative relative JJ cord-104239-xxlcdbqi 250 12 to to IN cord-104239-xxlcdbqi 250 13 its -PRON- PRP$ cord-104239-xxlcdbqi 250 14 location location NN cord-104239-xxlcdbqi 250 15 in in IN cord-104239-xxlcdbqi 250 16 sequential sequential JJ cord-104239-xxlcdbqi 250 17 concentric concentric JJ cord-104239-xxlcdbqi 250 18 shells shell NNS cord-104239-xxlcdbqi 250 19 of of IN cord-104239-xxlcdbqi 250 20 equal equal JJ cord-104239-xxlcdbqi 250 21 volume volume NN cord-104239-xxlcdbqi 250 22 ( ( -LRB- cord-104239-xxlcdbqi 250 23 Fig Fig NNP cord-104239-xxlcdbqi 250 24 . . NNP cord-104239-xxlcdbqi 250 25 6 6 CD cord-104239-xxlcdbqi 250 26 , , , cord-104239-xxlcdbqi 250 27 open open JJ cord-104239-xxlcdbqi 250 28 circles circle NNS cord-104239-xxlcdbqi 250 29 ) ) -RRB- cord-104239-xxlcdbqi 250 30 , , , cord-104239-xxlcdbqi 250 31 or or CC cord-104239-xxlcdbqi 250 32 relative relative JJ cord-104239-xxlcdbqi 250 33 to to IN cord-104239-xxlcdbqi 250 34 the the DT cord-104239-xxlcdbqi 250 35 diameter diameter NN cord-104239-xxlcdbqi 250 36 of of IN cord-104239-xxlcdbqi 250 37 the the DT cord-104239-xxlcdbqi 250 38 outermost outermost JJ cord-104239-xxlcdbqi 250 39 surface surface NN cord-104239-xxlcdbqi 250 40 of of IN cord-104239-xxlcdbqi 250 41 a a DT cord-104239-xxlcdbqi 250 42 given give VBN cord-104239-xxlcdbqi 250 43 shell shell NN cord-104239-xxlcdbqi 250 44 in in IN cord-104239-xxlcdbqi 250 45 which which WDT cord-104239-xxlcdbqi 250 46 a a DT cord-104239-xxlcdbqi 250 47 bud bud NN cord-104239-xxlcdbqi 250 48 is be VBZ cord-104239-xxlcdbqi 250 49 found find VBN cord-104239-xxlcdbqi 250 50 ( ( -LRB- cord-104239-xxlcdbqi 250 51 Fig Fig NNP cord-104239-xxlcdbqi 250 52 . . NNP cord-104239-xxlcdbqi 250 53 6 6 CD cord-104239-xxlcdbqi 250 54 , , , cord-104239-xxlcdbqi 250 55 closed closed JJ cord-104239-xxlcdbqi 250 56 circles circle NNS cord-104239-xxlcdbqi 250 57 ) ) -RRB- cord-104239-xxlcdbqi 250 58 . . . cord-104239-xxlcdbqi 251 1 It -PRON- PRP cord-104239-xxlcdbqi 251 2 is be VBZ cord-104239-xxlcdbqi 251 3 clear clear JJ cord-104239-xxlcdbqi 251 4 that that IN cord-104239-xxlcdbqi 251 5 the the DT cord-104239-xxlcdbqi 251 6 distribution distribution NN cord-104239-xxlcdbqi 251 7 did do VBD cord-104239-xxlcdbqi 251 8 not not RB cord-104239-xxlcdbqi 251 9 follow follow VB cord-104239-xxlcdbqi 251 10 that that DT cord-104239-xxlcdbqi 251 11 predicted predict VBD cord-104239-xxlcdbqi 251 12 for for IN cord-104239-xxlcdbqi 251 13 a a DT cord-104239-xxlcdbqi 251 14 strictly strictly RB cord-104239-xxlcdbqi 251 15 random random JJ cord-104239-xxlcdbqi 251 16 budding budding JJ cord-104239-xxlcdbqi 251 17 event event NN cord-104239-xxlcdbqi 251 18 . . . cord-104239-xxlcdbqi 252 1 The the DT cord-104239-xxlcdbqi 252 2 probability probability NN cord-104239-xxlcdbqi 252 3 of of IN cord-104239-xxlcdbqi 252 4 detecting detect VBG cord-104239-xxlcdbqi 252 5 a a DT cord-104239-xxlcdbqi 252 6 second second JJ cord-104239-xxlcdbqi 252 7 bud bud NN cord-104239-xxlcdbqi 252 8 within within IN cord-104239-xxlcdbqi 252 9 the the DT cord-104239-xxlcdbqi 252 10 first first JJ cord-104239-xxlcdbqi 252 11 50 50 CD cord-104239-xxlcdbqi 252 12 consecutive consecutive JJ cord-104239-xxlcdbqi 252 13 shells shell NNS cord-104239-xxlcdbqi 252 14 having have VBG cord-104239-xxlcdbqi 252 15 up up IN cord-104239-xxlcdbqi 252 16 to to IN cord-104239-xxlcdbqi 252 17 a a DT cord-104239-xxlcdbqi 252 18 0.6 0.6 CD cord-104239-xxlcdbqi 252 19 - - HYPH cord-104239-xxlcdbqi 252 20 1~m 1~m CD cord-104239-xxlcdbqi 252 21 outer outer JJ cord-104239-xxlcdbqi 252 22 shell shell NN cord-104239-xxlcdbqi 252 23 diameter diameter NN cord-104239-xxlcdbqi 252 24 markedly markedly RB cord-104239-xxlcdbqi 252 25 exceeded exceed VBD cord-104239-xxlcdbqi 252 26 that that DT cord-104239-xxlcdbqi 252 27 of of IN cord-104239-xxlcdbqi 252 28 a a DT cord-104239-xxlcdbqi 252 29 random random JJ cord-104239-xxlcdbqi 252 30 distribution distribution NN cord-104239-xxlcdbqi 252 31 ( ( -LRB- cord-104239-xxlcdbqi 252 32 Fig Fig NNP cord-104239-xxlcdbqi 252 33 . . . cord-104239-xxlcdbqi 252 34 6 6 CD cord-104239-xxlcdbqi 252 35 ) ) -RRB- cord-104239-xxlcdbqi 252 36 . . . cord-104239-xxlcdbqi 253 1 After after IN cord-104239-xxlcdbqi 253 2 a a DT cord-104239-xxlcdbqi 253 3 plateau plateau NN cord-104239-xxlcdbqi 253 4 at at IN cord-104239-xxlcdbqi 253 5 a a DT cord-104239-xxlcdbqi 253 6 value value NN cord-104239-xxlcdbqi 253 7 similar similar JJ cord-104239-xxlcdbqi 253 8 to to IN cord-104239-xxlcdbqi 253 9 that that DT cord-104239-xxlcdbqi 253 10 calculated calculate VBN cord-104239-xxlcdbqi 253 11 for for IN cord-104239-xxlcdbqi 253 12 a a DT cord-104239-xxlcdbqi 253 13 random random JJ cord-104239-xxlcdbqi 253 14 distribution distribution NN cord-104239-xxlcdbqi 253 15 ( ( -LRB- cord-104239-xxlcdbqi 253 16 up up IN cord-104239-xxlcdbqi 253 17 to to IN cord-104239-xxlcdbqi 253 18 an an DT cord-104239-xxlcdbqi 253 19 outer outer JJ cord-104239-xxlcdbqi 253 20 diameter diameter NN cord-104239-xxlcdbqi 253 21 of of IN cord-104239-xxlcdbqi 253 22 0.8 0.8 CD cord-104239-xxlcdbqi 253 23 Ixm Ixm NNP cord-104239-xxlcdbqi 253 24 or or CC cord-104239-xxlcdbqi 253 25 100 100 CD cord-104239-xxlcdbqi 253 26 shells shell NNS cord-104239-xxlcdbqi 253 27 ) ) -RRB- cord-104239-xxlcdbqi 253 28 , , , cord-104239-xxlcdbqi 253 29 the the DT cord-104239-xxlcdbqi 253 30 probability probability NN cord-104239-xxlcdbqi 253 31 fell fall VBD cord-104239-xxlcdbqi 253 32 dramatically dramatically RB cord-104239-xxlcdbqi 253 33 within within IN cord-104239-xxlcdbqi 253 34 the the DT cord-104239-xxlcdbqi 253 35 three three CD cord-104239-xxlcdbqi 253 36 - - HYPH cord-104239-xxlcdbqi 253 37 dimensional dimensional JJ cord-104239-xxlcdbqi 253 38 space space NN cord-104239-xxlcdbqi 253 39 defined define VBN cord-104239-xxlcdbqi 253 40 by by IN cord-104239-xxlcdbqi 253 41 the the DT cord-104239-xxlcdbqi 253 42 outermost outermost JJS cord-104239-xxlcdbqi 253 43 shell shell NN cord-104239-xxlcdbqi 253 44 examined examine VBN cord-104239-xxlcdbqi 253 45 ( ( -LRB- cord-104239-xxlcdbqi 253 46 ~1.2 ~1.2 CD cord-104239-xxlcdbqi 253 47 txm txm NNP cord-104239-xxlcdbqi 253 48 ) ) -RRB- cord-104239-xxlcdbqi 253 49 . . . cord-104239-xxlcdbqi 254 1 We -PRON- PRP cord-104239-xxlcdbqi 254 2 conclude conclude VBP cord-104239-xxlcdbqi 254 3 that that IN cord-104239-xxlcdbqi 254 4 budding budding NN cord-104239-xxlcdbqi 254 5 is be VBZ cord-104239-xxlcdbqi 254 6 not not RB cord-104239-xxlcdbqi 254 7 a a DT cord-104239-xxlcdbqi 254 8 random random JJ cord-104239-xxlcdbqi 254 9 event event NN cord-104239-xxlcdbqi 254 10 along along IN cord-104239-xxlcdbqi 254 11 the the DT cord-104239-xxlcdbqi 254 12 surface surface NN cord-104239-xxlcdbqi 254 13 of of IN cord-104239-xxlcdbqi 254 14 the the DT cord-104239-xxlcdbqi 254 15 ER er NN cord-104239-xxlcdbqi 254 16 , , , cord-104239-xxlcdbqi 254 17 but but CC cord-104239-xxlcdbqi 254 18 rather rather RB cord-104239-xxlcdbqi 254 19 is be VBZ cord-104239-xxlcdbqi 254 20 remarkably remarkably RB cord-104239-xxlcdbqi 254 21 restricted restricted JJ cord-104239-xxlcdbqi 254 22 to to IN cord-104239-xxlcdbqi 254 23 regional regional JJ cord-104239-xxlcdbqi 254 24 hot hot JJ cord-104239-xxlcdbqi 254 25 spots spot NNS cord-104239-xxlcdbqi 254 26 of of IN cord-104239-xxlcdbqi 254 27 budding bud VBG cord-104239-xxlcdbqi 254 28 activity activity NN cord-104239-xxlcdbqi 254 29 associated associate VBN cord-104239-xxlcdbqi 254 30 with with IN cord-104239-xxlcdbqi 254 31 export export NN cord-104239-xxlcdbqi 254 32 complexes complex NNS cord-104239-xxlcdbqi 254 33 . . . cord-104239-xxlcdbqi 255 1 As as IN cord-104239-xxlcdbqi 255 2 illustrated illustrate VBN cord-104239-xxlcdbqi 255 3 in in IN cord-104239-xxlcdbqi 255 4 Fig Fig NNP cord-104239-xxlcdbqi 255 5 . . . cord-104239-xxlcdbqi 256 1 4 4 LS cord-104239-xxlcdbqi 256 2 , , , cord-104239-xxlcdbqi 256 3 ER ER NNP cord-104239-xxlcdbqi 256 4 - - HYPH cord-104239-xxlcdbqi 256 5 budding bud VBG cord-104239-xxlcdbqi 256 6 profles profles NNP cord-104239-xxlcdbqi 256 7 predomi predomi NNP cord-104239-xxlcdbqi 256 8 - - : cord-104239-xxlcdbqi 256 9 nately nately RB cord-104239-xxlcdbqi 256 10 faced face VBD cord-104239-xxlcdbqi 256 11 into into IN cord-104239-xxlcdbqi 256 12 an an DT cord-104239-xxlcdbqi 256 13 area area NN cord-104239-xxlcdbqi 256 14 filled fill VBN cord-104239-xxlcdbqi 256 15 with with IN cord-104239-xxlcdbqi 256 16 VTCs vtc NNS cord-104239-xxlcdbqi 256 17 in in IN cord-104239-xxlcdbqi 256 18 vivo vivo NN cord-104239-xxlcdbqi 256 19 . . . cord-104239-xxlcdbqi 257 1 VTCs VTCs NNP cord-104239-xxlcdbqi 257 2 , , , cord-104239-xxlcdbqi 257 3 defined define VBN cord-104239-xxlcdbqi 257 4 as as IN cord-104239-xxlcdbqi 257 5 a a DT cord-104239-xxlcdbqi 257 6 group group NN cord-104239-xxlcdbqi 257 7 of of IN cord-104239-xxlcdbqi 257 8 four four CD cord-104239-xxlcdbqi 257 9 or or CC cord-104239-xxlcdbqi 257 10 more more JJR cord-104239-xxlcdbqi 257 11 60 60 CD cord-104239-xxlcdbqi 257 12 - - SYM cord-104239-xxlcdbqi 257 13 80-nm 80-nm CD cord-104239-xxlcdbqi 257 14 vesicular vesicular JJ cord-104239-xxlcdbqi 257 15 profiles profile NNS cord-104239-xxlcdbqi 257 16 with with IN cord-104239-xxlcdbqi 257 17 a a DT cord-104239-xxlcdbqi 257 18 characteristic characteristic JJ cord-104239-xxlcdbqi 257 19 shape shape NN cord-104239-xxlcdbqi 257 20 and and CC cord-104239-xxlcdbqi 257 21 coat coat NN cord-104239-xxlcdbqi 257 22 , , , cord-104239-xxlcdbqi 257 23 were be VBD cord-104239-xxlcdbqi 257 24 never never RB cord-104239-xxlcdbqi 257 25 detected detect VBN cord-104239-xxlcdbqi 257 26 in in IN cord-104239-xxlcdbqi 257 27 regions region NNS cord-104239-xxlcdbqi 257 28 lacking lack VBG cord-104239-xxlcdbqi 257 29 adjacent adjacent JJ cord-104239-xxlcdbqi 257 30 ER ER NNP cord-104239-xxlcdbqi 257 31 buds bud NNS cord-104239-xxlcdbqi 257 32 . . . cord-104239-xxlcdbqi 258 1 The the DT cord-104239-xxlcdbqi 258 2 membrane membrane NN cord-104239-xxlcdbqi 258 3 profiles profile NNS cord-104239-xxlcdbqi 258 4 of of IN cord-104239-xxlcdbqi 258 5 VTCs vtc NNS cord-104239-xxlcdbqi 258 6 were be VBD cord-104239-xxlcdbqi 258 7 distinct distinct JJ cord-104239-xxlcdbqi 258 8 from from IN cord-104239-xxlcdbqi 258 9 the the DT cord-104239-xxlcdbqi 258 10 ER er NN cord-104239-xxlcdbqi 258 11 and and CC cord-104239-xxlcdbqi 258 12 found find VBD cord-104239-xxlcdbqi 258 13 to to TO cord-104239-xxlcdbqi 258 14 be be VB cord-104239-xxlcdbqi 258 15 more more RBR cord-104239-xxlcdbqi 258 16 variable variable JJ cord-104239-xxlcdbqi 258 17 in in IN cord-104239-xxlcdbqi 258 18 size size NN cord-104239-xxlcdbqi 258 19 compared compare VBN cord-104239-xxlcdbqi 258 20 to to IN cord-104239-xxlcdbqi 258 21 the the DT cord-104239-xxlcdbqi 258 22 rather rather RB cord-104239-xxlcdbqi 258 23 homogeneous homogeneous JJ cord-104239-xxlcdbqi 258 24 appearance appearance NN cord-104239-xxlcdbqi 258 25 of of IN cord-104239-xxlcdbqi 258 26 buds bud NNS cord-104239-xxlcdbqi 258 27 emanating emanate VBG cord-104239-xxlcdbqi 258 28 from from IN cord-104239-xxlcdbqi 258 29 the the DT cord-104239-xxlcdbqi 258 30 ER ER NNP cord-104239-xxlcdbqi 258 31 surface surface NN cord-104239-xxlcdbqi 258 32 . . . cord-104239-xxlcdbqi 259 1 The the DT cord-104239-xxlcdbqi 259 2 average average JJ cord-104239-xxlcdbqi 259 3 diameter diameter NN cord-104239-xxlcdbqi 259 4 of of IN cord-104239-xxlcdbqi 259 5 VTCs vtc NNS cord-104239-xxlcdbqi 259 6 based base VBN cord-104239-xxlcdbqi 259 7 on on IN cord-104239-xxlcdbqi 259 8 serialthin serialthin NNP cord-104239-xxlcdbqi 259 9 sections section NNS cord-104239-xxlcdbqi 259 10 was be VBD cord-104239-xxlcdbqi 259 11 found find VBN cord-104239-xxlcdbqi 259 12 to to TO cord-104239-xxlcdbqi 259 13 be be VB cord-104239-xxlcdbqi 259 14 ~0.4 ~0.4 CD cord-104239-xxlcdbqi 260 1 Cm Cm NNP cord-104239-xxlcdbqi 260 2 ( ( -LRB- cord-104239-xxlcdbqi 260 3 Table Table NNP cord-104239-xxlcdbqi 260 4 II ii CD cord-104239-xxlcdbqi 260 5 ) ) -RRB- cord-104239-xxlcdbqi 260 6 with with IN cord-104239-xxlcdbqi 260 7 a a DT cord-104239-xxlcdbqi 260 8 range range NN cord-104239-xxlcdbqi 260 9 that that WDT cord-104239-xxlcdbqi 260 10 varied varied JJ cord-104239-xxlcdbqi 260 11 from from IN cord-104239-xxlcdbqi 261 1 ~0.2 ~0.2 CD cord-104239-xxlcdbqi 262 1 ~m ~m JJ cord-104239-xxlcdbqi 262 2 to to IN cord-104239-xxlcdbqi 262 3 > > XX cord-104239-xxlcdbqi 263 1 1 1 LS cord-104239-xxlcdbqi 264 1 ~m ~m JJ cord-104239-xxlcdbqi 265 1 ( ( -LRB- cord-104239-xxlcdbqi 265 2 Fig Fig NNP cord-104239-xxlcdbqi 265 3 . . . cord-104239-xxlcdbqi 265 4 5 5 CD cord-104239-xxlcdbqi 265 5 B b NN cord-104239-xxlcdbqi 265 6 ) ) -RRB- cord-104239-xxlcdbqi 265 7 . . . cord-104239-xxlcdbqi 266 1 Although although IN cord-104239-xxlcdbqi 266 2 technical technical JJ cord-104239-xxlcdbqi 266 3 limitations limitation NNS cord-104239-xxlcdbqi 266 4 do do VBP cord-104239-xxlcdbqi 266 5 not not RB cord-104239-xxlcdbqi 266 6 allow allow VB cord-104239-xxlcdbqi 266 7 us -PRON- PRP cord-104239-xxlcdbqi 266 8 to to TO cord-104239-xxlcdbqi 266 9 make make VB cord-104239-xxlcdbqi 266 10 a a DT cord-104239-xxlcdbqi 266 11 complete complete JJ cord-104239-xxlcdbqi 266 12 three three CD cord-104239-xxlcdbqi 266 13 - - HYPH cord-104239-xxlcdbqi 266 14 dimensional dimensional JJ cord-104239-xxlcdbqi 266 15 reconstruction reconstruction NN cord-104239-xxlcdbqi 266 16 of of IN cord-104239-xxlcdbqi 266 17 membrane membrane NN cord-104239-xxlcdbqi 266 18 continuities continuity NNS cord-104239-xxlcdbqi 266 19 between between IN cord-104239-xxlcdbqi 266 20 elements element NNS cord-104239-xxlcdbqi 266 21 of of IN cord-104239-xxlcdbqi 266 22 VTCs vtc NNS cord-104239-xxlcdbqi 266 23 , , , cord-104239-xxlcdbqi 266 24 individual individual JJ cord-104239-xxlcdbqi 266 25 profiles profile NNS cord-104239-xxlcdbqi 266 26 often often RB cord-104239-xxlcdbqi 266 27 appeared appear VBD cord-104239-xxlcdbqi 266 28 to to TO cord-104239-xxlcdbqi 266 29 be be VB cord-104239-xxlcdbqi 266 30 continuous continuous JJ cord-104239-xxlcdbqi 266 31 in in IN cord-104239-xxlcdbqi 266 32 several several JJ cord-104239-xxlcdbqi 266 33 consecutive consecutive JJ cord-104239-xxlcdbqi 266 34 sections section NNS cord-104239-xxlcdbqi 266 35 and and CC cord-104239-xxlcdbqi 266 36 consisted consist VBD cord-104239-xxlcdbqi 266 37 of of IN cord-104239-xxlcdbqi 266 38 short short JJ cord-104239-xxlcdbqi 266 39 tubules tubule NNS cord-104239-xxlcdbqi 266 40 that that WDT cord-104239-xxlcdbqi 266 41 could could MD cord-104239-xxlcdbqi 266 42 be be VB cord-104239-xxlcdbqi 266 43 connected connect VBN cord-104239-xxlcdbqi 266 44 together together RB cord-104239-xxlcdbqi 266 45 in in IN cord-104239-xxlcdbqi 266 46 a a DT cord-104239-xxlcdbqi 266 47 more more RBR cord-104239-xxlcdbqi 266 48 fenestrated fenestrated JJ cord-104239-xxlcdbqi 266 49 structure structure NN cord-104239-xxlcdbqi 266 50 ( ( -LRB- cord-104239-xxlcdbqi 266 51 Fig Fig NNP cord-104239-xxlcdbqi 266 52 . . NNP cord-104239-xxlcdbqi 266 53 1 1 CD cord-104239-xxlcdbqi 266 54 , , , cord-104239-xxlcdbqi 266 55 asterisks asterisk NNS cord-104239-xxlcdbqi 266 56 ) ) -RRB- cord-104239-xxlcdbqi 266 57 . . . cord-104239-xxlcdbqi 267 1 Tubules tubule NNS cord-104239-xxlcdbqi 267 2 within within IN cord-104239-xxlcdbqi 267 3 VTCs vtc NNS cord-104239-xxlcdbqi 267 4 characteristically characteristically RB cord-104239-xxlcdbqi 267 5 had have VBD cord-104239-xxlcdbqi 267 6 a a DT cord-104239-xxlcdbqi 267 7 dense dense JJ cord-104239-xxlcdbqi 267 8 COPI COPI NNP cord-104239-xxlcdbqi 267 9 - - HYPH cord-104239-xxlcdbqi 267 10 like like JJ cord-104239-xxlcdbqi 267 11 coat coat NN cord-104239-xxlcdbqi 267 12 at at IN cord-104239-xxlcdbqi 267 13 their -PRON- PRP$ cord-104239-xxlcdbqi 267 14 tips tip NNS cord-104239-xxlcdbqi 267 15 ( ( -LRB- cord-104239-xxlcdbqi 267 16 Figs fig NNS cord-104239-xxlcdbqi 267 17 . . . cord-104239-xxlcdbqi 268 1 1 1 CD cord-104239-xxlcdbqi 268 2 and and CC cord-104239-xxlcdbqi 268 3 3 3 CD cord-104239-xxlcdbqi 268 4 , , , cord-104239-xxlcdbqi 268 5 arrows arrow NNS cord-104239-xxlcdbqi 268 6 ) ) -RRB- cord-104239-xxlcdbqi 269 1 ( ( -LRB- cord-104239-xxlcdbqi 269 2 Melancon Melancon NNP cord-104239-xxlcdbqi 269 3 et et NNP cord-104239-xxlcdbqi 269 4 al al NNP cord-104239-xxlcdbqi 269 5 . . NNP cord-104239-xxlcdbqi 269 6 , , , cord-104239-xxlcdbqi 269 7 1987 1987 CD cord-104239-xxlcdbqi 270 1 ; ; : cord-104239-xxlcdbqi 270 2 Orci Orci NNP cord-104239-xxlcdbqi 270 3 et et NNP cord-104239-xxlcdbqi 270 4 al al NNP cord-104239-xxlcdbqi 270 5 . . NNP cord-104239-xxlcdbqi 270 6 , , , cord-104239-xxlcdbqi 270 7 1993a 1993a CD cord-104239-xxlcdbqi 270 8 ) ) -RRB- cord-104239-xxlcdbqi 270 9 . . . cord-104239-xxlcdbqi 271 1 The the DT cord-104239-xxlcdbqi 271 2 presence presence NN cord-104239-xxlcdbqi 271 3 of of IN cord-104239-xxlcdbqi 271 4 a a DT cord-104239-xxlcdbqi 271 5 COPI COPI NNP cord-104239-xxlcdbqi 271 6 - - HYPH cord-104239-xxlcdbqi 271 7 like like JJ cord-104239-xxlcdbqi 271 8 coat coat NN cord-104239-xxlcdbqi 271 9 within within IN cord-104239-xxlcdbqi 271 10 VTCs VTCs NNP cord-104239-xxlcdbqi 271 11 is be VBZ cord-104239-xxlcdbqi 271 12 consistent consistent JJ cord-104239-xxlcdbqi 271 13 with with IN cord-104239-xxlcdbqi 271 14 the the DT cord-104239-xxlcdbqi 271 15 numerous numerous JJ cord-104239-xxlcdbqi 271 16 morphological morphological JJ cord-104239-xxlcdbqi 271 17 studies study NNS cord-104239-xxlcdbqi 271 18 that that WDT cord-104239-xxlcdbqi 271 19 have have VBP cord-104239-xxlcdbqi 271 20 demonstrated demonstrate VBN cord-104239-xxlcdbqi 271 21 that that IN cord-104239-xxlcdbqi 271 22 these these DT cord-104239-xxlcdbqi 271 23 pre pre JJ cord-104239-xxlcdbqi 271 24 - - JJ cord-104239-xxlcdbqi 271 25 Golgi golgi JJ cord-104239-xxlcdbqi 271 26 intermediates intermediate NNS cord-104239-xxlcdbqi 271 27 are be VBP cord-104239-xxlcdbqi 271 28 a a DT cord-104239-xxlcdbqi 271 29 major major JJ cord-104239-xxlcdbqi 271 30 site site NN cord-104239-xxlcdbqi 271 31 of of IN cord-104239-xxlcdbqi 271 32 COPI COPI NNP cord-104239-xxlcdbqi 271 33 localization localization NN cord-104239-xxlcdbqi 271 34 when when WRB cord-104239-xxlcdbqi 271 35 examined examine VBN cord-104239-xxlcdbqi 271 36 using use VBG cord-104239-xxlcdbqi 271 37 indirect indirect JJ cord-104239-xxlcdbqi 271 38 immunofluorescence immunofluorescence NN cord-104239-xxlcdbqi 271 39 ( ( -LRB- cord-104239-xxlcdbqi 271 40 Lippincott Lippincott NNP cord-104239-xxlcdbqi 271 41 - - HYPH cord-104239-xxlcdbqi 271 42 Schwartz Schwartz NNP cord-104239-xxlcdbqi 271 43 , , , cord-104239-xxlcdbqi 271 44 1993 1993 CD cord-104239-xxlcdbqi 271 45 ; ; : cord-104239-xxlcdbqi 271 46 Aridor Aridor NNP cord-104239-xxlcdbqi 271 47 et et NNP cord-104239-xxlcdbqi 271 48 al al NNP cord-104239-xxlcdbqi 271 49 . . NNP cord-104239-xxlcdbqi 271 50 , , , cord-104239-xxlcdbqi 271 51 1995 1995 CD cord-104239-xxlcdbqi 271 52 ) ) -RRB- cord-104239-xxlcdbqi 271 53 or or CC cord-104239-xxlcdbqi 271 54 TEM TEM NNP cord-104239-xxlcdbqi 271 55 ( ( -LRB- cord-104239-xxlcdbqi 271 56 Oprins Oprins NNP cord-104239-xxlcdbqi 271 57 et et NNP cord-104239-xxlcdbqi 271 58 al al NNP cord-104239-xxlcdbqi 271 59 . . NNP cord-104239-xxlcdbqi 271 60 , , , cord-104239-xxlcdbqi 271 61 1993 1993 CD cord-104239-xxlcdbqi 271 62 ; ; : cord-104239-xxlcdbqi 272 1 Pind Pind NNP cord-104239-xxlcdbqi 272 2 et et FW cord-104239-xxlcdbqi 272 3 al al NNP cord-104239-xxlcdbqi 272 4 . . . cord-104239-xxlcdbqi 273 1 , , , cord-104239-xxlcdbqi 273 2 1994a 1994a CD cord-104239-xxlcdbqi 273 3 ; ; : cord-104239-xxlcdbqi 273 4 Griffiths Griffiths NNP cord-104239-xxlcdbqi 273 5 et et NNP cord-104239-xxlcdbqi 273 6 al al NNP cord-104239-xxlcdbqi 273 7 . . NNP cord-104239-xxlcdbqi 273 8 , , , cord-104239-xxlcdbqi 273 9 1995b 1995b CD cord-104239-xxlcdbqi 273 10 ) ) -RRB- cord-104239-xxlcdbqi 273 11 . . . cord-104239-xxlcdbqi 274 1 To to TO cord-104239-xxlcdbqi 274 2 estimate estimate VB cord-104239-xxlcdbqi 274 3 the the DT cord-104239-xxlcdbqi 274 4 total total JJ cord-104239-xxlcdbqi 274 5 number number NN cord-104239-xxlcdbqi 274 6 of of IN cord-104239-xxlcdbqi 274 7 elements element NNS cord-104239-xxlcdbqi 274 8 within within IN cord-104239-xxlcdbqi 274 9 a a DT cord-104239-xxlcdbqi 274 10 VTC VTC NNP cord-104239-xxlcdbqi 274 11 , , , cord-104239-xxlcdbqi 274 12 we -PRON- PRP cord-104239-xxlcdbqi 274 13 assumed assume VBD cord-104239-xxlcdbqi 274 14 that that IN cord-104239-xxlcdbqi 274 15 each each DT cord-104239-xxlcdbqi 274 16 profile profile NN cord-104239-xxlcdbqi 274 17 appearing appear VBG cord-104239-xxlcdbqi 274 18 in in IN cord-104239-xxlcdbqi 274 19 an an DT cord-104239-xxlcdbqi 274 20 individual individual JJ cord-104239-xxlcdbqi 274 21 section section NN cord-104239-xxlcdbqi 274 22 was be VBD cord-104239-xxlcdbqi 274 23 , , , cord-104239-xxlcdbqi 274 24 in in IN cord-104239-xxlcdbqi 274 25 fact fact NN cord-104239-xxlcdbqi 274 26 , , , cord-104239-xxlcdbqi 274 27 a a DT cord-104239-xxlcdbqi 274 28 separate separate JJ cord-104239-xxlcdbqi 274 29 vesicle vesicle NN cord-104239-xxlcdbqi 274 30 or or CC cord-104239-xxlcdbqi 274 31 tubule tubule NN cord-104239-xxlcdbqi 274 32 . . . cord-104239-xxlcdbqi 275 1 From from IN cord-104239-xxlcdbqi 275 2 this this DT cord-104239-xxlcdbqi 275 3 assumption assumption NN cord-104239-xxlcdbqi 275 4 , , , cord-104239-xxlcdbqi 275 5 the the DT cord-104239-xxlcdbqi 275 6 average average JJ cord-104239-xxlcdbqi 275 7 number number NN cord-104239-xxlcdbqi 275 8 of of IN cord-104239-xxlcdbqi 275 9 elements element NNS cord-104239-xxlcdbqi 275 10 was be VBD cord-104239-xxlcdbqi 275 11 determined determine VBN cord-104239-xxlcdbqi 275 12 to to TO cord-104239-xxlcdbqi 275 13 be be VB cord-104239-xxlcdbqi 275 14 32 32 CD cord-104239-xxlcdbqi 275 15 + + CD cord-104239-xxlcdbqi 276 1 _ _ NNP cord-104239-xxlcdbqi 277 1 _ _ NN cord-104239-xxlcdbqi 277 2 4 4 CD cord-104239-xxlcdbqi 278 1 ( ( -LRB- cord-104239-xxlcdbqi 278 2 Table Table NNP cord-104239-xxlcdbqi 278 3 II II NNP cord-104239-xxlcdbqi 278 4 ) ) -RRB- cord-104239-xxlcdbqi 278 5 . . . cord-104239-xxlcdbqi 279 1 Clusters cluster NNS cord-104239-xxlcdbqi 279 2 generally generally RB cord-104239-xxlcdbqi 279 3 contained contain VBN cord-104239-xxlcdbqi 279 4 between between IN cord-104239-xxlcdbqi 279 5 10 10 CD cord-104239-xxlcdbqi 279 6 and and CC cord-104239-xxlcdbqi 279 7 60 60 CD cord-104239-xxlcdbqi 279 8 vesicular vesicular JJ cord-104239-xxlcdbqi 279 9 - - HYPH cord-104239-xxlcdbqi 279 10 tubular tubular JJ cord-104239-xxlcdbqi 279 11 elements element NNS cord-104239-xxlcdbqi 279 12 , , , cord-104239-xxlcdbqi 279 13 although although IN cord-104239-xxlcdbqi 279 14 some some DT cord-104239-xxlcdbqi 279 15 of of IN cord-104239-xxlcdbqi 279 16 the the DT cord-104239-xxlcdbqi 279 17 clusters cluster NNS cord-104239-xxlcdbqi 279 18 contained contain VBN cord-104239-xxlcdbqi 279 19 as as RB cord-104239-xxlcdbqi 279 20 many many JJ cord-104239-xxlcdbqi 279 21 as as IN cord-104239-xxlcdbqi 279 22 150 150 CD cord-104239-xxlcdbqi 279 23 elements element NNS cord-104239-xxlcdbqi 279 24 ( ( -LRB- cord-104239-xxlcdbqi 279 25 Fig Fig NNP cord-104239-xxlcdbqi 279 26 . . . cord-104239-xxlcdbqi 279 27 5 5 CD cord-104239-xxlcdbqi 279 28 C C NNP cord-104239-xxlcdbqi 279 29 ) ) -RRB- cord-104239-xxlcdbqi 279 30 . . . cord-104239-xxlcdbqi 280 1 Although although IN cord-104239-xxlcdbqi 280 2 these these DT cord-104239-xxlcdbqi 280 3 values value NNS cord-104239-xxlcdbqi 280 4 are be VBP cord-104239-xxlcdbqi 280 5 undoubtedly undoubtedly RB cord-104239-xxlcdbqi 280 6 an an DT cord-104239-xxlcdbqi 280 7 overestimate overestimate NN cord-104239-xxlcdbqi 280 8 of of IN cord-104239-xxlcdbqi 280 9 the the DT cord-104239-xxlcdbqi 280 10 total total JJ cord-104239-xxlcdbqi 280 11 number number NN cord-104239-xxlcdbqi 280 12 of of IN cord-104239-xxlcdbqi 280 13 luminally luminally RB cord-104239-xxlcdbqi 280 14 distinct distinct JJ cord-104239-xxlcdbqi 280 15 elements element NNS cord-104239-xxlcdbqi 280 16 , , , cord-104239-xxlcdbqi 280 17 given give VBN cord-104239-xxlcdbqi 280 18 that that IN cord-104239-xxlcdbqi 280 19 tubules tubule NNS cord-104239-xxlcdbqi 280 20 within within IN cord-104239-xxlcdbqi 280 21 VTCs vtc NNS cord-104239-xxlcdbqi 280 22 are be VBP cord-104239-xxlcdbqi 280 23 likely likely JJ cord-104239-xxlcdbqi 280 24 to to TO cord-104239-xxlcdbqi 280 25 extend extend VB cord-104239-xxlcdbqi 280 26 across across IN cord-104239-xxlcdbqi 280 27 several several JJ cord-104239-xxlcdbqi 280 28 consecutive consecutive JJ cord-104239-xxlcdbqi 280 29 thin thin JJ cord-104239-xxlcdbqi 280 30 - - HYPH cord-104239-xxlcdbqi 280 31 sections section NNS cord-104239-xxlcdbqi 280 32 , , , cord-104239-xxlcdbqi 280 33 they -PRON- PRP cord-104239-xxlcdbqi 280 34 serve serve VBP cord-104239-xxlcdbqi 280 35 as as IN cord-104239-xxlcdbqi 280 36 a a DT cord-104239-xxlcdbqi 280 37 useful useful JJ cord-104239-xxlcdbqi 280 38 numerical numerical JJ cord-104239-xxlcdbqi 280 39 approximation approximation NN cord-104239-xxlcdbqi 280 40 for for IN cord-104239-xxlcdbqi 280 41 the the DT cord-104239-xxlcdbqi 280 42 relative relative JJ cord-104239-xxlcdbqi 280 43 size size NN cord-104239-xxlcdbqi 280 44 and and CC cord-104239-xxlcdbqi 280 45 composition composition NN cord-104239-xxlcdbqi 280 46 of of IN cord-104239-xxlcdbqi 280 47 VTCs vtc NNS cord-104239-xxlcdbqi 280 48 . . . cord-104239-xxlcdbqi 281 1 When when WRB cord-104239-xxlcdbqi 281 2 we -PRON- PRP cord-104239-xxlcdbqi 281 3 compared compare VBD cord-104239-xxlcdbqi 281 4 the the DT cord-104239-xxlcdbqi 281 5 number number NN cord-104239-xxlcdbqi 281 6 of of IN cord-104239-xxlcdbqi 281 7 elements element NNS cord-104239-xxlcdbqi 281 8 found find VBN cord-104239-xxlcdbqi 281 9 in in IN cord-104239-xxlcdbqi 281 10 peripheral peripheral NN cord-104239-xxlcdbqi 281 11 vs vs IN cord-104239-xxlcdbqi 281 12 Golgi Golgi NNP cord-104239-xxlcdbqi 281 13 - - HYPH cord-104239-xxlcdbqi 281 14 adjacent adjacent JJ cord-104239-xxlcdbqi 281 15 VTCs vtc NNS cord-104239-xxlcdbqi 281 16 , , , cord-104239-xxlcdbqi 281 17 we -PRON- PRP cord-104239-xxlcdbqi 281 18 observed observe VBD cord-104239-xxlcdbqi 281 19 very very RB cord-104239-xxlcdbqi 281 20 similar similar JJ cord-104239-xxlcdbqi 281 21 values value NNS cord-104239-xxlcdbqi 281 22 , , , cord-104239-xxlcdbqi 281 23 suggesting suggest VBG cord-104239-xxlcdbqi 281 24 they -PRON- PRP cord-104239-xxlcdbqi 281 25 are be VBP cord-104239-xxlcdbqi 281 26 of of IN cord-104239-xxlcdbqi 281 27 comparable comparable JJ cord-104239-xxlcdbqi 281 28 size size NN cord-104239-xxlcdbqi 281 29 ( ( -LRB- cord-104239-xxlcdbqi 281 30 Fig fig NN cord-104239-xxlcdbqi 281 31 . . . cord-104239-xxlcdbqi 281 32 5 5 CD cord-104239-xxlcdbqi 281 33 C C NNP cord-104239-xxlcdbqi 281 34 ) ) -RRB- cord-104239-xxlcdbqi 281 35 . . . cord-104239-xxlcdbqi 282 1 Whereas whereas IN cord-104239-xxlcdbqi 282 2 peripheral peripheral JJ cord-104239-xxlcdbqi 282 3 VTCs vtc NNS cord-104239-xxlcdbqi 282 4 were be VBD cord-104239-xxlcdbqi 282 5 generally generally RB cord-104239-xxlcdbqi 282 6 spherical spherical JJ cord-104239-xxlcdbqi 282 7 in in IN cord-104239-xxlcdbqi 282 8 shape shape NN cord-104239-xxlcdbqi 282 9 , , , cord-104239-xxlcdbqi 282 10 those those DT cord-104239-xxlcdbqi 282 11 adjacent adjacent JJ cord-104239-xxlcdbqi 282 12 to to IN cord-104239-xxlcdbqi 282 13 Golgi Golgi NNP cord-104239-xxlcdbqi 282 14 possessed possess VBD cord-104239-xxlcdbqi 282 15 a a DT cord-104239-xxlcdbqi 282 16 semispherical semispherical JJ cord-104239-xxlcdbqi 282 17 shape shape NN cord-104239-xxlcdbqi 282 18 with with IN cord-104239-xxlcdbqi 282 19 the the DT cord-104239-xxlcdbqi 282 20 CGN CGN NNP cord-104239-xxlcdbqi 282 21 occupying occupy VBG cord-104239-xxlcdbqi 282 22 one one CD cord-104239-xxlcdbqi 282 23 hemisphere hemisphere NN cord-104239-xxlcdbqi 282 24 , , , cord-104239-xxlcdbqi 282 25 and and CC cord-104239-xxlcdbqi 282 26 the the DT cord-104239-xxlcdbqi 282 27 other other JJ cord-104239-xxlcdbqi 282 28 hemispheres hemisphere NNS cord-104239-xxlcdbqi 282 29 facing face VBG cord-104239-xxlcdbqi 282 30 ER ER NNP cord-104239-xxlcdbqi 282 31 membranes membrane NNS cord-104239-xxlcdbqi 282 32 . . . cord-104239-xxlcdbqi 283 1 Using use VBG cord-104239-xxlcdbqi 283 2 the the DT cord-104239-xxlcdbqi 283 3 disector disector NN cord-104239-xxlcdbqi 283 4 method method NN cord-104239-xxlcdbqi 283 5 to to TO cord-104239-xxlcdbqi 283 6 estimate estimate VB cord-104239-xxlcdbqi 283 7 the the DT cord-104239-xxlcdbqi 283 8 total total JJ cord-104239-xxlcdbqi 283 9 number number NN cord-104239-xxlcdbqi 283 10 of of IN cord-104239-xxlcdbqi 283 11 VTCs vtc NNS cord-104239-xxlcdbqi 283 12 per per IN cord-104239-xxlcdbqi 283 13 cell cell NN cord-104239-xxlcdbqi 283 14 ( ( -LRB- cord-104239-xxlcdbqi 283 15 Sterio Sterio NNP cord-104239-xxlcdbqi 283 16 , , , cord-104239-xxlcdbqi 283 17 1984 1984 CD cord-104239-xxlcdbqi 283 18 ) ) -RRB- cord-104239-xxlcdbqi 283 19 ( ( -LRB- cord-104239-xxlcdbqi 283 20 see see VB cord-104239-xxlcdbqi 283 21 Materials material NNS cord-104239-xxlcdbqi 283 22 and and CC cord-104239-xxlcdbqi 283 23 Methods Methods NNPS cord-104239-xxlcdbqi 283 24 ) ) -RRB- cord-104239-xxlcdbqi 283 25 , , , cord-104239-xxlcdbqi 283 26 we -PRON- PRP cord-104239-xxlcdbqi 283 27 found find VBD cord-104239-xxlcdbqi 283 28 an an DT cord-104239-xxlcdbqi 283 29 average average JJ cord-104239-xxlcdbqi 283 30 value value NN cord-104239-xxlcdbqi 283 31 of of IN cord-104239-xxlcdbqi 283 32 74 74 CD cord-104239-xxlcdbqi 283 33 _ _ NN cord-104239-xxlcdbqi 283 34 11 11 CD cord-104239-xxlcdbqi 283 35 clusters cluster NNS cord-104239-xxlcdbqi 283 36 per per IN cord-104239-xxlcdbqi 283 37 cell cell NN cord-104239-xxlcdbqi 283 38 . . . cord-104239-xxlcdbqi 284 1 This this DT cord-104239-xxlcdbqi 284 2 level level NN cord-104239-xxlcdbqi 284 3 is be VBZ cord-104239-xxlcdbqi 284 4 similar similar JJ cord-104239-xxlcdbqi 284 5 to to IN cord-104239-xxlcdbqi 284 6 that that DT cord-104239-xxlcdbqi 284 7 observed observe VBD cord-104239-xxlcdbqi 284 8 using use VBG cord-104239-xxlcdbqi 284 9 more more JJR cord-104239-xxlcdbqi 284 10 indirect indirect JJ cord-104239-xxlcdbqi 284 11 TEM tem NN cord-104239-xxlcdbqi 284 12 methods method NNS cord-104239-xxlcdbqi 284 13 ( ( -LRB- cord-104239-xxlcdbqi 284 14 Buccione Buccione NNP cord-104239-xxlcdbqi 284 15 et et NNP cord-104239-xxlcdbqi 284 16 al al NNP cord-104239-xxlcdbqi 284 17 . . NNP cord-104239-xxlcdbqi 284 18 , , , cord-104239-xxlcdbqi 284 19 1996 1996 CD cord-104239-xxlcdbqi 284 20 ) ) -RRB- cord-104239-xxlcdbqi 284 21 or or CC cord-104239-xxlcdbqi 284 22 indirect indirect JJ cord-104239-xxlcdbqi 284 23 immunofluorescence immunofluorescence NN cord-104239-xxlcdbqi 284 24 in in IN cord-104239-xxlcdbqi 284 25 other other JJ cord-104239-xxlcdbqi 284 26 cell cell NN cord-104239-xxlcdbqi 284 27 lines line NNS cord-104239-xxlcdbqi 285 1 ( ( -LRB- cord-104239-xxlcdbqi 285 2 Plutner Plutner NNP cord-104239-xxlcdbqi 285 3 et et NNP cord-104239-xxlcdbqi 285 4 al al NNP cord-104239-xxlcdbqi 285 5 . . NNP cord-104239-xxlcdbqi 285 6 , , , cord-104239-xxlcdbqi 285 7 1992 1992 CD cord-104239-xxlcdbqi 285 8 ; ; . cord-104239-xxlcdbqi 286 1 Aridor Aridor NNP cord-104239-xxlcdbqi 286 2 et et NNP cord-104239-xxlcdbqi 286 3 al al NNP cord-104239-xxlcdbqi 286 4 . . NNP cord-104239-xxlcdbqi 286 5 , , , cord-104239-xxlcdbqi 286 6 1995 1995 CD cord-104239-xxlcdbqi 286 7 ) ) -RRB- cord-104239-xxlcdbqi 286 8 . . . cord-104239-xxlcdbqi 287 1 To to TO cord-104239-xxlcdbqi 287 2 correlate correlate VB cord-104239-xxlcdbqi 287 3 our -PRON- PRP$ cord-104239-xxlcdbqi 287 4 morphological morphological JJ cord-104239-xxlcdbqi 287 5 observations observation NNS cord-104239-xxlcdbqi 287 6 with with IN cord-104239-xxlcdbqi 287 7 previous previous JJ cord-104239-xxlcdbqi 287 8 in in IN cord-104239-xxlcdbqi 287 9 vitro vitro FW cord-104239-xxlcdbqi 287 10 biochemical biochemical JJ cord-104239-xxlcdbqi 287 11 studies study NNS cord-104239-xxlcdbqi 287 12 , , , cord-104239-xxlcdbqi 287 13 we -PRON- PRP cord-104239-xxlcdbqi 287 14 used use VBD cord-104239-xxlcdbqi 287 15 semi semi JJ cord-104239-xxlcdbqi 287 16 - - JJ cord-104239-xxlcdbqi 287 17 intact intact JJ cord-104239-xxlcdbqi 287 18 NRK NRK NNP cord-104239-xxlcdbqi 287 19 cells cell NNS cord-104239-xxlcdbqi 287 20 to to TO cord-104239-xxlcdbqi 287 21 examine examine VB cord-104239-xxlcdbqi 287 22 the the DT cord-104239-xxlcdbqi 287 23 potential potential JJ cord-104239-xxlcdbqi 287 24 role role NN cord-104239-xxlcdbqi 287 25 of of IN cord-104239-xxlcdbqi 287 26 COPII COPII NNP cord-104239-xxlcdbqi 287 27 components component NNS cord-104239-xxlcdbqi 287 28 in in IN cord-104239-xxlcdbqi 287 29 the the DT cord-104239-xxlcdbqi 287 30 formation formation NN cord-104239-xxlcdbqi 287 31 of of IN cord-104239-xxlcdbqi 287 32 ER er NN cord-104239-xxlcdbqi 287 33 - - HYPH cord-104239-xxlcdbqi 287 34 derived derive VBN cord-104239-xxlcdbqi 287 35 buds bud NNS cord-104239-xxlcdbqi 287 36 and and CC cord-104239-xxlcdbqi 287 37 VTCs vtc NNS cord-104239-xxlcdbqi 287 38 ( ( -LRB- cord-104239-xxlcdbqi 287 39 Plutner Plutner NNP cord-104239-xxlcdbqi 287 40 et et NNP cord-104239-xxlcdbqi 287 41 al al NNP cord-104239-xxlcdbqi 287 42 . . NNP cord-104239-xxlcdbqi 287 43 , , , cord-104239-xxlcdbqi 287 44 1992 1992 CD cord-104239-xxlcdbqi 287 45 ; ; : cord-104239-xxlcdbqi 287 46 Peter Peter NNP cord-104239-xxlcdbqi 287 47 et et NNP cord-104239-xxlcdbqi 287 48 al al NNP cord-104239-xxlcdbqi 287 49 . . NNP cord-104239-xxlcdbqi 287 50 , , , cord-104239-xxlcdbqi 287 51 1993 1993 CD cord-104239-xxlcdbqi 287 52 ; ; : cord-104239-xxlcdbqi 287 53 Balch Balch NNP cord-104239-xxlcdbqi 287 54 et et NNP cord-104239-xxlcdbqi 287 55 al al NNP cord-104239-xxlcdbqi 287 56 . . NNP cord-104239-xxlcdbqi 287 57 , , , cord-104239-xxlcdbqi 287 58 1994 1994 CD cord-104239-xxlcdbqi 287 59 ; ; : cord-104239-xxlcdbqi 287 60 Pind Pind NNP cord-104239-xxlcdbqi 287 61 et et NNP cord-104239-xxlcdbqi 287 62 al al NNP cord-104239-xxlcdbqi 287 63 . . NNP cord-104239-xxlcdbqi 287 64 , , , cord-104239-xxlcdbqi 287 65 1994b 1994b CD cord-104239-xxlcdbqi 287 66 ) ) -RRB- cord-104239-xxlcdbqi 287 67 . . . cord-104239-xxlcdbqi 288 1 Semi semi JJ cord-104239-xxlcdbqi 288 2 - - JJ cord-104239-xxlcdbqi 288 3 intact intact JJ cord-104239-xxlcdbqi 288 4 cells cell NNS cord-104239-xxlcdbqi 288 5 incubated incubate VBN cord-104239-xxlcdbqi 288 6 for for IN cord-104239-xxlcdbqi 288 7 30 30 CD cord-104239-xxlcdbqi 288 8 min min NN cord-104239-xxlcdbqi 288 9 at at IN cord-104239-xxlcdbqi 288 10 32 32 CD cord-104239-xxlcdbqi 288 11 ° ° NN cord-104239-xxlcdbqi 288 12 C c NN cord-104239-xxlcdbqi 288 13 in in IN cord-104239-xxlcdbqi 288 14 Figure Figure NNP cord-104239-xxlcdbqi 288 15 4 4 CD cord-104239-xxlcdbqi 288 16 . . . cord-104239-xxlcdbqi 289 1 Three three CD cord-104239-xxlcdbqi 289 2 - - HYPH cord-104239-xxlcdbqi 289 3 dimensional dimensional JJ cord-104239-xxlcdbqi 289 4 reconstruction reconstruction NN cord-104239-xxlcdbqi 289 5 of of IN cord-104239-xxlcdbqi 289 6 a a DT cord-104239-xxlcdbqi 289 7 peripheral peripheral JJ cord-104239-xxlcdbqi 289 8 export export NN cord-104239-xxlcdbqi 289 9 complex complex NN cord-104239-xxlcdbqi 289 10 . . . cord-104239-xxlcdbqi 290 1 Membrane membrane NN cord-104239-xxlcdbqi 290 2 contours contour NNS cord-104239-xxlcdbqi 290 3 shown show VBN cord-104239-xxlcdbqi 290 4 in in IN cord-104239-xxlcdbqi 290 5 Fig Fig NNP cord-104239-xxlcdbqi 290 6 . . . cord-104239-xxlcdbqi 291 1 3 3 CD cord-104239-xxlcdbqi 291 2 are be VBP cord-104239-xxlcdbqi 291 3 illustrated illustrate VBN cord-104239-xxlcdbqi 291 4 at at IN cord-104239-xxlcdbqi 291 5 a a DT cord-104239-xxlcdbqi 291 6 magnification magnification NN cord-104239-xxlcdbqi 291 7 of of IN cord-104239-xxlcdbqi 291 8 160,000 160,000 CD cord-104239-xxlcdbqi 291 9 . . . cord-104239-xxlcdbqi 292 1 Transparencies transparency NNS cord-104239-xxlcdbqi 292 2 containing contain VBG cord-104239-xxlcdbqi 292 3 the the DT cord-104239-xxlcdbqi 292 4 membrane membrane NN cord-104239-xxlcdbqi 292 5 contours contour NNS cord-104239-xxlcdbqi 292 6 were be VBD cord-104239-xxlcdbqi 292 7 scanned scan VBN cord-104239-xxlcdbqi 292 8 , , , cord-104239-xxlcdbqi 292 9 overlayed overlay VBN cord-104239-xxlcdbqi 292 10 , , , cord-104239-xxlcdbqi 292 11 and and CC cord-104239-xxlcdbqi 292 12 coaligned coaligne VBD cord-104239-xxlcdbqi 292 13 based base VBN cord-104239-xxlcdbqi 292 14 on on IN cord-104239-xxlcdbqi 292 15 the the DT cord-104239-xxlcdbqi 292 16 position position NN cord-104239-xxlcdbqi 292 17 of of IN cord-104239-xxlcdbqi 292 18 mitochrondria mitochrondria NNP cord-104239-xxlcdbqi 292 19 and and CC cord-104239-xxlcdbqi 292 20 ER ER NNP cord-104239-xxlcdbqi 292 21 . . . cord-104239-xxlcdbqi 293 1 Green Green NNP cord-104239-xxlcdbqi 293 2 denotes denote VBZ cord-104239-xxlcdbqi 293 3 ER ER NNP cord-104239-xxlcdbqi 293 4 cisternae cisternae NNP cord-104239-xxlcdbqi 293 5 , , , cord-104239-xxlcdbqi 293 6 blue blue JJ cord-104239-xxlcdbqi 293 7 denotes denote VBZ cord-104239-xxlcdbqi 293 8 ER ER NNP cord-104239-xxlcdbqi 293 9 buds bud NNS cord-104239-xxlcdbqi 293 10 , , , cord-104239-xxlcdbqi 293 11 and and CC cord-104239-xxlcdbqi 293 12 red red NN cord-104239-xxlcdbqi 293 13 denotes denote VBZ cord-104239-xxlcdbqi 293 14 tubules tubule NNS cord-104239-xxlcdbqi 293 15 and and CC cord-104239-xxlcdbqi 293 16 vesicles vesicle NNS cord-104239-xxlcdbqi 293 17 of of IN cord-104239-xxlcdbqi 293 18 VTCs vtc NNS cord-104239-xxlcdbqi 293 19 . . . cord-104239-xxlcdbqi 294 1 Vesicular vesicular JJ cord-104239-xxlcdbqi 294 2 membrane membrane NN cord-104239-xxlcdbqi 294 3 contours contour NNS cord-104239-xxlcdbqi 294 4 within within IN cord-104239-xxlcdbqi 294 5 the the DT cord-104239-xxlcdbqi 294 6 VTC VTC NNP cord-104239-xxlcdbqi 295 1 whose whose WP$ cord-104239-xxlcdbqi 295 2 luminal luminal JJ cord-104239-xxlcdbqi 295 3 continuity continuity NN cord-104239-xxlcdbqi 295 4 to to IN cord-104239-xxlcdbqi 295 5 the the DT cord-104239-xxlcdbqi 295 6 surrounding surround VBG cord-104239-xxlcdbqi 295 7 ER ER NNP cord-104239-xxlcdbqi 295 8 membranes membrane NNS cord-104239-xxlcdbqi 295 9 was be VBD cord-104239-xxlcdbqi 295 10 evident evident JJ cord-104239-xxlcdbqi 295 11 in in IN cord-104239-xxlcdbqi 295 12 consecutive consecutive JJ cord-104239-xxlcdbqi 295 13 sections section NNS cord-104239-xxlcdbqi 295 14 were be VBD cord-104239-xxlcdbqi 295 15 denoted denote VBN cord-104239-xxlcdbqi 295 16 in in IN cord-104239-xxlcdbqi 295 17 green green JJ cord-104239-xxlcdbqi 295 18 . . . cord-104239-xxlcdbqi 296 1 More more RBR cord-104239-xxlcdbqi 296 2 intense intense JJ cord-104239-xxlcdbqi 296 3 shades shade NNS cord-104239-xxlcdbqi 296 4 of of IN cord-104239-xxlcdbqi 296 5 the the DT cord-104239-xxlcdbqi 296 6 same same JJ cord-104239-xxlcdbqi 296 7 color color NN cord-104239-xxlcdbqi 296 8 reflect reflect VBP cord-104239-xxlcdbqi 296 9 distance distance NN cord-104239-xxlcdbqi 296 10 from from IN cord-104239-xxlcdbqi 296 11 the the DT cord-104239-xxlcdbqi 296 12 uppermost uppermost JJ cord-104239-xxlcdbqi 296 13 section section NN cord-104239-xxlcdbqi 296 14 . . . cord-104239-xxlcdbqi 297 1 The the DT cord-104239-xxlcdbqi 297 2 alveolate alveolate JJ cord-104239-xxlcdbqi 297 3 coats coat NNS cord-104239-xxlcdbqi 297 4 of of IN cord-104239-xxlcdbqi 297 5 ER ER NNP cord-104239-xxlcdbqi 297 6 buds bud NNS cord-104239-xxlcdbqi 297 7 , , , cord-104239-xxlcdbqi 297 8 where where WRB cord-104239-xxlcdbqi 297 9 evident evident JJ cord-104239-xxlcdbqi 297 10 , , , cord-104239-xxlcdbqi 297 11 are be VBP cord-104239-xxlcdbqi 297 12 dictated dictate VBN cord-104239-xxlcdbqi 297 13 by by IN cord-104239-xxlcdbqi 297 14 stipples stipple NNS cord-104239-xxlcdbqi 297 15 . . . cord-104239-xxlcdbqi 298 1 Images image NNS cord-104239-xxlcdbqi 298 2 are be VBP cord-104239-xxlcdbqi 298 3 presented present VBN cord-104239-xxlcdbqi 298 4 either either CC cord-104239-xxlcdbqi 298 5 individually individually RB cord-104239-xxlcdbqi 298 6 with with IN cord-104239-xxlcdbqi 298 7 the the DT cord-104239-xxlcdbqi 298 8 section section NN cord-104239-xxlcdbqi 298 9 number number NN cord-104239-xxlcdbqi 298 10 indicated indicate VBD cord-104239-xxlcdbqi 298 11 ( ( -LRB- cord-104239-xxlcdbqi 298 12 bottom bottom JJ cord-104239-xxlcdbqi 298 13 two two CD cord-104239-xxlcdbqi 298 14 rows row NNS cord-104239-xxlcdbqi 298 15 ) ) -RRB- cord-104239-xxlcdbqi 298 16 , , , cord-104239-xxlcdbqi 298 17 or or CC cord-104239-xxlcdbqi 298 18 as as IN cord-104239-xxlcdbqi 298 19 an an DT cord-104239-xxlcdbqi 298 20 overlay overlay NN cord-104239-xxlcdbqi 298 21 containing contain VBG cord-104239-xxlcdbqi 298 22 four four CD cord-104239-xxlcdbqi 298 23 images image NNS cord-104239-xxlcdbqi 298 24 ( ( -LRB- cord-104239-xxlcdbqi 298 25 left leave VBN cord-104239-xxlcdbqi 298 26 ) ) -RRB- cord-104239-xxlcdbqi 298 27 for for IN cord-104239-xxlcdbqi 298 28 clarity clarity NN cord-104239-xxlcdbqi 298 29 or or CC cord-104239-xxlcdbqi 298 30 all all DT cord-104239-xxlcdbqi 298 31 eight eight CD cord-104239-xxlcdbqi 298 32 images image NNS cord-104239-xxlcdbqi 298 33 ( ( -LRB- cord-104239-xxlcdbqi 298 34 right right RB cord-104239-xxlcdbqi 298 35 ) ) -RRB- cord-104239-xxlcdbqi 298 36 . . . cord-104239-xxlcdbqi 299 1 The the DT cord-104239-xxlcdbqi 299 2 resulting result VBG cord-104239-xxlcdbqi 299 3 reconstruction reconstruction NN cord-104239-xxlcdbqi 299 4 shows show VBZ cord-104239-xxlcdbqi 299 5 the the DT cord-104239-xxlcdbqi 299 6 clustered clustered JJ cord-104239-xxlcdbqi 299 7 structure structure NN cord-104239-xxlcdbqi 299 8 of of IN cord-104239-xxlcdbqi 299 9 a a DT cord-104239-xxlcdbqi 299 10 typical typical JJ cord-104239-xxlcdbqi 299 11 VTC vtc NN cord-104239-xxlcdbqi 299 12 , , , cord-104239-xxlcdbqi 299 13 surrounded surround VBN cord-104239-xxlcdbqi 299 14 by by IN cord-104239-xxlcdbqi 299 15 ER er NN cord-104239-xxlcdbqi 299 16 - - HYPH cord-104239-xxlcdbqi 299 17 bearing bear VBG cord-104239-xxlcdbqi 299 18 buds bud NNS cord-104239-xxlcdbqi 299 19 occasionally occasionally RB cord-104239-xxlcdbqi 299 20 penetrating penetrate VBG cord-104239-xxlcdbqi 299 21 the the DT cord-104239-xxlcdbqi 299 22 periphery periphery NN cord-104239-xxlcdbqi 299 23 of of IN cord-104239-xxlcdbqi 299 24 a a DT cord-104239-xxlcdbqi 299 25 VTC vtc NN cord-104239-xxlcdbqi 299 26 , , , cord-104239-xxlcdbqi 299 27 as as IN cord-104239-xxlcdbqi 299 28 evidenced evidence VBN cord-104239-xxlcdbqi 299 29 by by IN cord-104239-xxlcdbqi 299 30 the the DT cord-104239-xxlcdbqi 299 31 coated coated JJ cord-104239-xxlcdbqi 299 32 portions portion NNS cord-104239-xxlcdbqi 299 33 of of IN cord-104239-xxlcdbqi 299 34 ER ER NNP cord-104239-xxlcdbqi 299 35 tubules tubule NNS cord-104239-xxlcdbqi 299 36 ( ( -LRB- cord-104239-xxlcdbqi 299 37 blue blue JJ cord-104239-xxlcdbqi 299 38 ) ) -RRB- cord-104239-xxlcdbqi 299 39 adjacent adjacent JJ cord-104239-xxlcdbqi 299 40 to to IN cord-104239-xxlcdbqi 299 41 VTC VTC NNP cord-104239-xxlcdbqi 299 42 tubules tubule NNS cord-104239-xxlcdbqi 299 43 ( ( -LRB- cord-104239-xxlcdbqi 299 44 red red JJ cord-104239-xxlcdbqi 299 45 ) ) -RRB- cord-104239-xxlcdbqi 299 46 . . . cord-104239-xxlcdbqi 300 1 the the DT cord-104239-xxlcdbqi 300 2 absence absence NN cord-104239-xxlcdbqi 300 3 of of IN cord-104239-xxlcdbqi 300 4 cytosol cytosol NN cord-104239-xxlcdbqi 300 5 failed fail VBD cord-104239-xxlcdbqi 300 6 to to TO cord-104239-xxlcdbqi 300 7 generate generate VB cord-104239-xxlcdbqi 300 8 detectable detectable JJ cord-104239-xxlcdbqi 300 9 VTCs vtc NNS cord-104239-xxlcdbqi 300 10 ( ( -LRB- cord-104239-xxlcdbqi 300 11 not not RB cord-104239-xxlcdbqi 300 12 shown show VBN cord-104239-xxlcdbqi 300 13 ) ) -RRB- cord-104239-xxlcdbqi 300 14 . . . cord-104239-xxlcdbqi 301 1 This this DT cord-104239-xxlcdbqi 301 2 result result NN cord-104239-xxlcdbqi 301 3 is be VBZ cord-104239-xxlcdbqi 301 4 consistent consistent JJ cord-104239-xxlcdbqi 301 5 with with IN cord-104239-xxlcdbqi 301 6 the the DT cord-104239-xxlcdbqi 301 7 fact fact NN cord-104239-xxlcdbqi 301 8 that that IN cord-104239-xxlcdbqi 301 9 preexisting preexist VBG cord-104239-xxlcdbqi 301 10 VTCs vtc NNS cord-104239-xxlcdbqi 301 11 are be VBP cord-104239-xxlcdbqi 301 12 unstable unstable JJ cord-104239-xxlcdbqi 301 13 during during IN cord-104239-xxlcdbqi 301 14 permeabilization permeabilization NN cord-104239-xxlcdbqi 302 1 ( ( -LRB- cord-104239-xxlcdbqi 302 2 Aridot Aridot NNP cord-104239-xxlcdbqi 302 3 et et FW cord-104239-xxlcdbqi 302 4 al al NNP cord-104239-xxlcdbqi 302 5 . . NNP cord-104239-xxlcdbqi 302 6 , , , cord-104239-xxlcdbqi 302 7 1995 1995 CD cord-104239-xxlcdbqi 302 8 ) ) -RRB- cord-104239-xxlcdbqi 302 9 and and CC cord-104239-xxlcdbqi 302 10 that that IN cord-104239-xxlcdbqi 302 11 cytosol cytosol NN cord-104239-xxlcdbqi 302 12 contains contain VBZ cord-104239-xxlcdbqi 302 13 essential essential JJ cord-104239-xxlcdbqi 302 14 soluble soluble JJ cord-104239-xxlcdbqi 302 15 components component NNS cord-104239-xxlcdbqi 302 16 of of IN cord-104239-xxlcdbqi 302 17 the the DT cord-104239-xxlcdbqi 302 18 COPII COPII NNP cord-104239-xxlcdbqi 302 19 machinery machinery NN cord-104239-xxlcdbqi 302 20 required require VBN cord-104239-xxlcdbqi 302 21 for for IN cord-104239-xxlcdbqi 302 22 the the DT cord-104239-xxlcdbqi 302 23 export export NN cord-104239-xxlcdbqi 302 24 of of IN cord-104239-xxlcdbqi 302 25 cargo cargo NN cord-104239-xxlcdbqi 302 26 from from IN cord-104239-xxlcdbqi 302 27 the the DT cord-104239-xxlcdbqi 302 28 E e NN cord-104239-xxlcdbqi 302 29 R r NN cord-104239-xxlcdbqi 302 30 ( ( -LRB- cord-104239-xxlcdbqi 302 31 Barlowe Barlowe NNP cord-104239-xxlcdbqi 302 32 et et FW cord-104239-xxlcdbqi 302 33 al al NNP cord-104239-xxlcdbqi 302 34 . . NNP cord-104239-xxlcdbqi 302 35 , , , cord-104239-xxlcdbqi 302 36 1994 1994 CD cord-104239-xxlcdbqi 302 37 ; ; : cord-104239-xxlcdbqi 302 38 Kuge Kuge NNP cord-104239-xxlcdbqi 302 39 et et NNP cord-104239-xxlcdbqi 302 40 al al NNP cord-104239-xxlcdbqi 302 41 . . NNP cord-104239-xxlcdbqi 302 42 , , , cord-104239-xxlcdbqi 302 43 1994 1994 CD cord-104239-xxlcdbqi 302 44 ) ) -RRB- cord-104239-xxlcdbqi 302 45 . . . cord-104239-xxlcdbqi 303 1 VTCs VTCs NNP cord-104239-xxlcdbqi 303 2 generated generate VBN cord-104239-xxlcdbqi 303 3 in in IN cord-104239-xxlcdbqi 303 4 vitro vitro FW cord-104239-xxlcdbqi 303 5 in in IN cord-104239-xxlcdbqi 303 6 the the DT cord-104239-xxlcdbqi 303 7 presence presence NN cord-104239-xxlcdbqi 303 8 of of IN cord-104239-xxlcdbqi 303 9 cytosol cytosol NN cord-104239-xxlcdbqi 303 10 are be VBP cord-104239-xxlcdbqi 303 11 nearly nearly RB cord-104239-xxlcdbqi 303 12 identical identical JJ cord-104239-xxlcdbqi 303 13 to to IN cord-104239-xxlcdbqi 303 14 those those DT cord-104239-xxlcdbqi 303 15 observed observe VBN cord-104239-xxlcdbqi 303 16 in in IN cord-104239-xxlcdbqi 303 17 vivo vivo NNP cord-104239-xxlcdbqi 303 18 . . . cord-104239-xxlcdbqi 304 1 We -PRON- PRP cord-104239-xxlcdbqi 304 2 have have VBP cord-104239-xxlcdbqi 304 3 previously previously RB cord-104239-xxlcdbqi 304 4 shown show VBN cord-104239-xxlcdbqi 304 5 that that IN cord-104239-xxlcdbqi 304 6 they -PRON- PRP cord-104239-xxlcdbqi 304 7 are be VBP cord-104239-xxlcdbqi 304 8 composed compose VBN cord-104239-xxlcdbqi 304 9 of of IN cord-104239-xxlcdbqi 304 10 a a DT cord-104239-xxlcdbqi 304 11 compact compact JJ cord-104239-xxlcdbqi 304 12 network network NN cord-104239-xxlcdbqi 304 13 of of IN cord-104239-xxlcdbqi 304 14 tubules tubule NNS cord-104239-xxlcdbqi 304 15 and and CC cord-104239-xxlcdbqi 304 16 vesicles vesicle NNS cord-104239-xxlcdbqi 304 17 that that WDT cord-104239-xxlcdbqi 304 18 lack lack VBP cord-104239-xxlcdbqi 304 19 direct direct JJ cord-104239-xxlcdbqi 304 20 luminal luminal JJ cord-104239-xxlcdbqi 304 21 connections connection NNS cord-104239-xxlcdbqi 304 22 to to IN cord-104239-xxlcdbqi 304 23 E e NN cord-104239-xxlcdbqi 304 24 R r NN cord-104239-xxlcdbqi 304 25 membranes membrane NNS cord-104239-xxlcdbqi 304 26 and and CC cord-104239-xxlcdbqi 304 27 frequently frequently RB cord-104239-xxlcdbqi 304 28 label label VB cord-104239-xxlcdbqi 304 29 positively positively RB cord-104239-xxlcdbqi 304 30 for for IN cord-104239-xxlcdbqi 304 31 B b NN cord-104239-xxlcdbqi 304 32 - - HYPH cord-104239-xxlcdbqi 304 33 COP cop NN cord-104239-xxlcdbqi 304 34 using use VBG cord-104239-xxlcdbqi 304 35 immunoelectron immunoelectron NNP cord-104239-xxlcdbqi 304 36 microscopy microscopy NNP cord-104239-xxlcdbqi 305 1 Pind Pind NNP cord-104239-xxlcdbqi 305 2 et et NNP cord-104239-xxlcdbqi 305 3 al al NNP cord-104239-xxlcdbqi 305 4 . . NNP cord-104239-xxlcdbqi 305 5 , , , cord-104239-xxlcdbqi 305 6 1994a 1994a CD cord-104239-xxlcdbqi 305 7 ) ) -RRB- cord-104239-xxlcdbqi 305 8 . . . cord-104239-xxlcdbqi 306 1 However however RB cord-104239-xxlcdbqi 306 2 , , , cord-104239-xxlcdbqi 306 3 ER ER NNP cord-104239-xxlcdbqi 306 4 - - HYPH cord-104239-xxlcdbqi 306 5 budding bud VBG cord-104239-xxlcdbqi 306 6 profiles profile NNS cord-104239-xxlcdbqi 306 7 , , , cord-104239-xxlcdbqi 306 8 like like IN cord-104239-xxlcdbqi 306 9 those those DT cord-104239-xxlcdbqi 306 10 observed observe VBN cord-104239-xxlcdbqi 306 11 adjacent adjacent JJ cord-104239-xxlcdbqi 306 12 to to IN cord-104239-xxlcdbqi 306 13 VTCs vtc NNS cord-104239-xxlcdbqi 306 14 in in IN cord-104239-xxlcdbqi 306 15 vivo vivo NN cord-104239-xxlcdbqi 306 16 ( ( -LRB- cord-104239-xxlcdbqi 306 17 Figs Figs NNP cord-104239-xxlcdbqi 306 18 . . . cord-104239-xxlcdbqi 306 19 1 1 CD cord-104239-xxlcdbqi 306 20 - - SYM cord-104239-xxlcdbqi 306 21 3 3 CD cord-104239-xxlcdbqi 306 22 , , , cord-104239-xxlcdbqi 306 23 arrowheads arrowhead NNS cord-104239-xxlcdbqi 306 24 ) ) -RRB- cord-104239-xxlcdbqi 306 25 , , , cord-104239-xxlcdbqi 306 26 were be VBD cord-104239-xxlcdbqi 306 27 rarely rarely RB cord-104239-xxlcdbqi 306 28 observed observe VBN cord-104239-xxlcdbqi 306 29 in in IN cord-104239-xxlcdbqi 306 30 vitro vitro FW cord-104239-xxlcdbqi 306 31 . . . cord-104239-xxlcdbqi 307 1 Since since IN cord-104239-xxlcdbqi 307 2 previous previous JJ cord-104239-xxlcdbqi 307 3 studies study NNS cord-104239-xxlcdbqi 307 4 used use VBD cord-104239-xxlcdbqi 307 5 mild mild JJ cord-104239-xxlcdbqi 307 6 fixation fixation NN cord-104239-xxlcdbqi 307 7 conditions condition NNS cord-104239-xxlcdbqi 307 8 in in IN cord-104239-xxlcdbqi 307 9 conjunction conjunction NN cord-104239-xxlcdbqi 307 10 with with IN cord-104239-xxlcdbqi 307 11 an an DT cord-104239-xxlcdbqi 307 12 immunodiffusion immunodiffusion NN cord-104239-xxlcdbqi 307 13 procedure procedure NN cord-104239-xxlcdbqi 307 14 to to TO cord-104239-xxlcdbqi 307 15 label label VB cord-104239-xxlcdbqi 307 16 VSV VSV NNP cord-104239-xxlcdbqi 307 17 - - HYPH cord-104239-xxlcdbqi 307 18 G G NNP cord-104239-xxlcdbqi 307 19 in in IN cord-104239-xxlcdbqi 307 20 VTCs vtc NNS cord-104239-xxlcdbqi 307 21 , , , cord-104239-xxlcdbqi 307 22 we -PRON- PRP cord-104239-xxlcdbqi 307 23 reasoned reason VBD cord-104239-xxlcdbqi 307 24 that that IN cord-104239-xxlcdbqi 307 25 ER ER NNP cord-104239-xxlcdbqi 307 26 buds bud NNS cord-104239-xxlcdbqi 307 27 may may MD cord-104239-xxlcdbqi 307 28 be be VB cord-104239-xxlcdbqi 307 29 labile labile JJ cord-104239-xxlcdbqi 307 30 structures structure NNS cord-104239-xxlcdbqi 307 31 . . . cord-104239-xxlcdbqi 308 1 We -PRON- PRP cord-104239-xxlcdbqi 308 2 therefore therefore RB cord-104239-xxlcdbqi 308 3 applied apply VBD cord-104239-xxlcdbqi 308 4 more more RBR cord-104239-xxlcdbqi 308 5 stringent stringent JJ cord-104239-xxlcdbqi 308 6 fixation fixation NN cord-104239-xxlcdbqi 308 7 and and CC cord-104239-xxlcdbqi 308 8 embedding embed VBG cord-104239-xxlcdbqi 308 9 conditions condition NNS cord-104239-xxlcdbqi 308 10 to to TO cord-104239-xxlcdbqi 308 11 preserve preserve VB cord-104239-xxlcdbqi 308 12 ultrastrucrural ultrastrucrural JJ cord-104239-xxlcdbqi 308 13 details detail NNS cord-104239-xxlcdbqi 308 14 ( ( -LRB- cord-104239-xxlcdbqi 308 15 see see VB cord-104239-xxlcdbqi 308 16 Materials material NNS cord-104239-xxlcdbqi 308 17 and and CC cord-104239-xxlcdbqi 308 18 Methods Methods NNPS cord-104239-xxlcdbqi 308 19 ) ) -RRB- cord-104239-xxlcdbqi 308 20 . . . cord-104239-xxlcdbqi 309 1 Under under IN cord-104239-xxlcdbqi 309 2 these these DT cord-104239-xxlcdbqi 309 3 conditions condition NNS cord-104239-xxlcdbqi 309 4 , , , cord-104239-xxlcdbqi 309 5 ER ER NNP cord-104239-xxlcdbqi 309 6 buds bud NNS cord-104239-xxlcdbqi 309 7 were be VBD cord-104239-xxlcdbqi 309 8 observed observe VBN cord-104239-xxlcdbqi 309 9 that that WDT cord-104239-xxlcdbqi 309 10 had have VBD cord-104239-xxlcdbqi 309 11 a a DT cord-104239-xxlcdbqi 309 12 characteristic characteristic JJ cord-104239-xxlcdbqi 309 13 coat coat NN cord-104239-xxlcdbqi 309 14 resembling resemble VBG cord-104239-xxlcdbqi 309 15 those those DT cord-104239-xxlcdbqi 309 16 found find VBN cord-104239-xxlcdbqi 309 17 in in IN cord-104239-xxlcdbqi 309 18 vivo vivo NN cord-104239-xxlcdbqi 309 19 and and CC cord-104239-xxlcdbqi 309 20 were be VBD cord-104239-xxlcdbqi 309 21 only only RB cord-104239-xxlcdbqi 309 22 detected detect VBN cord-104239-xxlcdbqi 309 23 adjacent adjacent JJ cord-104239-xxlcdbqi 309 24 to to IN cord-104239-xxlcdbqi 309 25 VTCs VTCs NNP cord-104239-xxlcdbqi 309 26 ( ( -LRB- cord-104239-xxlcdbqi 309 27 not not RB cord-104239-xxlcdbqi 309 28 shown show VBN cord-104239-xxlcdbqi 309 29 ) ) -RRB- cord-104239-xxlcdbqi 309 30 , , , cord-104239-xxlcdbqi 309 31 suggesting suggest VBG cord-104239-xxlcdbqi 309 32 that that IN cord-104239-xxlcdbqi 309 33 even even RB cord-104239-xxlcdbqi 309 34 in in IN cord-104239-xxlcdbqi 309 35 semi semi JJ cord-104239-xxlcdbqi 309 36 - - JJ cord-104239-xxlcdbqi 309 37 intact intact JJ cord-104239-xxlcdbqi 309 38 ceils ceil NNS cord-104239-xxlcdbqi 309 39 , , , cord-104239-xxlcdbqi 309 40 budding bud VBG cord-104239-xxlcdbqi 309 41 is be VBZ cord-104239-xxlcdbqi 309 42 restricted restrict VBN cord-104239-xxlcdbqi 309 43 to to IN cord-104239-xxlcdbqi 309 44 specialized specialized JJ cord-104239-xxlcdbqi 309 45 regions region NNS cord-104239-xxlcdbqi 309 46 of of IN cord-104239-xxlcdbqi 309 47 the the DT cord-104239-xxlcdbqi 309 48 ER ER NNP cord-104239-xxlcdbqi 309 49 , , , cord-104239-xxlcdbqi 309 50 To to TO cord-104239-xxlcdbqi 309 51 examine examine VB cord-104239-xxlcdbqi 309 52 the the DT cord-104239-xxlcdbqi 309 53 formation formation NN cord-104239-xxlcdbqi 309 54 of of IN cord-104239-xxlcdbqi 309 55 buds bud NNS cord-104239-xxlcdbqi 309 56 and and CC cord-104239-xxlcdbqi 309 57 their -PRON- PRP$ cord-104239-xxlcdbqi 309 58 relationship relationship NN cord-104239-xxlcdbqi 309 59 to to IN cord-104239-xxlcdbqi 309 60 VTCs VTCs NNP cord-104239-xxlcdbqi 309 61 in in IN cord-104239-xxlcdbqi 309 62 vitro vitro FW cord-104239-xxlcdbqi 309 63 , , , cord-104239-xxlcdbqi 309 64 we -PRON- PRP cord-104239-xxlcdbqi 309 65 made make VBD cord-104239-xxlcdbqi 309 66 use use NN cord-104239-xxlcdbqi 309 67 of of IN cord-104239-xxlcdbqi 309 68 the the DT cord-104239-xxlcdbqi 309 69 nonhydrolyzable nonhydrolyzable JJ cord-104239-xxlcdbqi 309 70 analog analog NN cord-104239-xxlcdbqi 309 71 of of IN cord-104239-xxlcdbqi 309 72 GTP GTP NNP cord-104239-xxlcdbqi 309 73 , , , cord-104239-xxlcdbqi 309 74 GTP~/S GTP~/S NNP cord-104239-xxlcdbqi 309 75 , , , cord-104239-xxlcdbqi 309 76 which which WDT cord-104239-xxlcdbqi 309 77 permanently permanently RB cord-104239-xxlcdbqi 309 78 activates activate VBZ cord-104239-xxlcdbqi 309 79 Sarl Sarl NNP cord-104239-xxlcdbqi 309 80 and and CC cord-104239-xxlcdbqi 309 81 other other JJ cord-104239-xxlcdbqi 309 82 GTPases gtpase NNS cord-104239-xxlcdbqi 309 83 , , , cord-104239-xxlcdbqi 309 84 leading lead VBG cord-104239-xxlcdbqi 309 85 to to IN cord-104239-xxlcdbqi 309 86 stable stable JJ cord-104239-xxlcdbqi 309 87 coat coat NN cord-104239-xxlcdbqi 309 88 assembly assembly NN cord-104239-xxlcdbqi 309 89 and and CC cord-104239-xxlcdbqi 309 90 accumulation accumulation NN cord-104239-xxlcdbqi 309 91 of of IN cord-104239-xxlcdbqi 309 92 buds bud NNS cord-104239-xxlcdbqi 309 93 . . . cord-104239-xxlcdbqi 310 1 Interestingly interestingly RB cord-104239-xxlcdbqi 310 2 , , , cord-104239-xxlcdbqi 310 3 we -PRON- PRP cord-104239-xxlcdbqi 310 4 observed observe VBD cord-104239-xxlcdbqi 310 5 for for IN cord-104239-xxlcdbqi 310 6 the the DT cord-104239-xxlcdbqi 310 7 first first JJ cord-104239-xxlcdbqi 310 8 time time NN cord-104239-xxlcdbqi 310 9 using use VBG cord-104239-xxlcdbqi 310 10 strong strong JJ cord-104239-xxlcdbqi 310 11 fixation fixation NN cord-104239-xxlcdbqi 310 12 conditions condition NNS cord-104239-xxlcdbqi 310 13 that that WDT cord-104239-xxlcdbqi 310 14 nascent nascent JJ cord-104239-xxlcdbqi 310 15 budding bud VBG cord-104239-xxlcdbqi 310 16 profiles profile NNS cord-104239-xxlcdbqi 310 17 generated generate VBN cord-104239-xxlcdbqi 310 18 in in IN cord-104239-xxlcdbqi 310 19 the the DT cord-104239-xxlcdbqi 310 20 presence presence NN cord-104239-xxlcdbqi 310 21 of of IN cord-104239-xxlcdbqi 310 22 GTP~/S GTP~/S NNP cord-104239-xxlcdbqi 310 23 had have VBD cord-104239-xxlcdbqi 310 24 not not RB cord-104239-xxlcdbqi 310 25 only only RB cord-104239-xxlcdbqi 310 26 a a DT cord-104239-xxlcdbqi 310 27 cluster cluster NN cord-104239-xxlcdbqi 310 28 appearance appearance NN cord-104239-xxlcdbqi 311 1 ( ( -LRB- cord-104239-xxlcdbqi 311 2 Pind Pind NNP cord-104239-xxlcdbqi 311 3 et et NNP cord-104239-xxlcdbqi 311 4 al al NNP cord-104239-xxlcdbqi 311 5 . . NNP cord-104239-xxlcdbqi 311 6 , , , cord-104239-xxlcdbqi 311 7 1994a 1994a CD cord-104239-xxlcdbqi 311 8 ) ) -RRB- cord-104239-xxlcdbqi 311 9 , , , cord-104239-xxlcdbqi 311 10 but but CC cord-104239-xxlcdbqi 311 11 also also RB cord-104239-xxlcdbqi 311 12 frequently frequently RB cord-104239-xxlcdbqi 311 13 had have VBD cord-104239-xxlcdbqi 311 14 a a DT cord-104239-xxlcdbqi 311 15 distinctive distinctive JJ cord-104239-xxlcdbqi 311 16 " " `` cord-104239-xxlcdbqi 311 17 beaded beaded JJ cord-104239-xxlcdbqi 311 18 necklace necklace NN cord-104239-xxlcdbqi 311 19 " " '' cord-104239-xxlcdbqi 311 20 appearance appearance NN cord-104239-xxlcdbqi 311 21 with with IN cord-104239-xxlcdbqi 311 22 each each DT cord-104239-xxlcdbqi 311 23 vesicle vesicle NN cord-104239-xxlcdbqi 311 24 being be VBG cord-104239-xxlcdbqi 311 25 a a DT cord-104239-xxlcdbqi 311 26 bead bead NN cord-104239-xxlcdbqi 312 1 ( ( -LRB- cord-104239-xxlcdbqi 312 2 Fig Fig NNP cord-104239-xxlcdbqi 312 3 . . NNP cord-104239-xxlcdbqi 312 4 7 7 CD cord-104239-xxlcdbqi 312 5 , , , cord-104239-xxlcdbqi 312 6 B b NN cord-104239-xxlcdbqi 312 7 and and CC cord-104239-xxlcdbqi 312 8 C C NNP cord-104239-xxlcdbqi 312 9 ) ) -RRB- cord-104239-xxlcdbqi 312 10 . . . cord-104239-xxlcdbqi 313 1 The the DT cord-104239-xxlcdbqi 313 2 vesicles vesicle NNS cord-104239-xxlcdbqi 313 3 were be VBD cord-104239-xxlcdbqi 313 4 nearly nearly RB cord-104239-xxlcdbqi 313 5 identical identical JJ cord-104239-xxlcdbqi 313 6 in in IN cord-104239-xxlcdbqi 313 7 size size NN cord-104239-xxlcdbqi 313 8 but but CC cord-104239-xxlcdbqi 313 9 lacked lack VBD cord-104239-xxlcdbqi 313 10 luminal luminal JJ cord-104239-xxlcdbqi 313 11 continuity continuity NN cord-104239-xxlcdbqi 313 12 . . . cord-104239-xxlcdbqi 314 1 These these DT cord-104239-xxlcdbqi 314 2 strings string NNS cord-104239-xxlcdbqi 314 3 of of IN cord-104239-xxlcdbqi 314 4 vesicles vesicle NNS cord-104239-xxlcdbqi 314 5 were be VBD cord-104239-xxlcdbqi 314 6 similar similar JJ cord-104239-xxlcdbqi 314 7 to to IN cord-104239-xxlcdbqi 314 8 the the DT cord-104239-xxlcdbqi 314 9 shorter short JJR cord-104239-xxlcdbqi 314 10 necklaces necklace NNS cord-104239-xxlcdbqi 314 11 sometimes sometimes RB cord-104239-xxlcdbqi 314 12 observed observe VBD cord-104239-xxlcdbqi 314 13 in in IN cord-104239-xxlcdbqi 314 14 vivo vivo NN cord-104239-xxlcdbqi 314 15 under under IN cord-104239-xxlcdbqi 314 16 normal normal JJ cord-104239-xxlcdbqi 314 17 incubation incubation NN cord-104239-xxlcdbqi 314 18 conditions condition NNS cord-104239-xxlcdbqi 315 1 ( ( -LRB- cord-104239-xxlcdbqi 315 2 Fig Fig NNP cord-104239-xxlcdbqi 315 3 . . . cord-104239-xxlcdbqi 315 4 7 7 CD cord-104239-xxlcdbqi 315 5 A a NN cord-104239-xxlcdbqi 315 6 ) ) -RRB- cord-104239-xxlcdbqi 315 7 . . . cord-104239-xxlcdbqi 316 1 These these DT cord-104239-xxlcdbqi 316 2 necklaces necklace NNS cord-104239-xxlcdbqi 316 3 were be VBD cord-104239-xxlcdbqi 316 4 confined confine VBN cord-104239-xxlcdbqi 316 5 to to IN cord-104239-xxlcdbqi 316 6 only only RB cord-104239-xxlcdbqi 316 7 a a DT cord-104239-xxlcdbqi 316 8 small small JJ cord-104239-xxlcdbqi 316 9 fraction fraction NN cord-104239-xxlcdbqi 316 10 of of IN cord-104239-xxlcdbqi 316 11 the the DT cord-104239-xxlcdbqi 316 12 total total JJ cord-104239-xxlcdbqi 316 13 ER ER NNP cord-104239-xxlcdbqi 316 14 surface surface NN cord-104239-xxlcdbqi 316 15 . . . cord-104239-xxlcdbqi 317 1 By by IN cord-104239-xxlcdbqi 317 2 analyzing analyze VBG cord-104239-xxlcdbqi 317 3 sections section NNS cord-104239-xxlcdbqi 317 4 through through IN cord-104239-xxlcdbqi 317 5 > > XX cord-104239-xxlcdbqi 317 6 600 600 CD cord-104239-xxlcdbqi 317 7 individual individual JJ cord-104239-xxlcdbqi 317 8 necklaces necklace NNS cord-104239-xxlcdbqi 317 9 , , , cord-104239-xxlcdbqi 317 10 we -PRON- PRP cord-104239-xxlcdbqi 317 11 have have VBP cord-104239-xxlcdbqi 317 12 never never RB cord-104239-xxlcdbqi 317 13 detected detect VBN cord-104239-xxlcdbqi 317 14 them -PRON- PRP cord-104239-xxlcdbqi 317 15 to to TO cord-104239-xxlcdbqi 317 16 have have VB cord-104239-xxlcdbqi 317 17 more more JJR cord-104239-xxlcdbqi 317 18 than than IN cord-104239-xxlcdbqi 317 19 one one CD cord-104239-xxlcdbqi 317 20 connection connection NN cord-104239-xxlcdbqi 317 21 to to IN cord-104239-xxlcdbqi 317 22 the the DT cord-104239-xxlcdbqi 317 23 ER ER NNP cord-104239-xxlcdbqi 317 24 membrane membrane NN cord-104239-xxlcdbqi 317 25 , , , cord-104239-xxlcdbqi 317 26 supporting support VBG cord-104239-xxlcdbqi 317 27 the the DT cord-104239-xxlcdbqi 317 28 possibility possibility NN cord-104239-xxlcdbqi 317 29 that that IN cord-104239-xxlcdbqi 317 30 each each DT cord-104239-xxlcdbqi 317 31 string string NN cord-104239-xxlcdbqi 317 32 grows grow VBZ cord-104239-xxlcdbqi 317 33 from from IN cord-104239-xxlcdbqi 317 34 a a DT cord-104239-xxlcdbqi 317 35 local local JJ cord-104239-xxlcdbqi 317 36 area area NN cord-104239-xxlcdbqi 317 37 of of IN cord-104239-xxlcdbqi 317 38 budding bud VBG cord-104239-xxlcdbqi 317 39 activity activity NN cord-104239-xxlcdbqi 317 40 . . . cord-104239-xxlcdbqi 318 1 These these DT cord-104239-xxlcdbqi 318 2 observations observation NNS cord-104239-xxlcdbqi 318 3 suggest suggest VBP cord-104239-xxlcdbqi 318 4 that that IN cord-104239-xxlcdbqi 318 5 budding budding NN cord-104239-xxlcdbqi 318 6 continues continue VBZ cord-104239-xxlcdbqi 318 7 Outer outer JJ cord-104239-xxlcdbqi 318 8 diameter diameter NN cord-104239-xxlcdbqi 318 9 of of IN cord-104239-xxlcdbqi 318 10 shell shell NN cord-104239-xxlcdbqi 318 11 ( ( -LRB- cord-104239-xxlcdbqi 319 1 lID lid LS cord-104239-xxlcdbqi 319 2 ) ) -RRB- cord-104239-xxlcdbqi 319 3 Figure figure NN cord-104239-xxlcdbqi 319 4 6 6 CD cord-104239-xxlcdbqi 319 5 . . . cord-104239-xxlcdbqi 320 1 Probability probability NN cord-104239-xxlcdbqi 320 2 of of IN cord-104239-xxlcdbqi 320 3 a a DT cord-104239-xxlcdbqi 320 4 given give VBN cord-104239-xxlcdbqi 320 5 bud bud NN cord-104239-xxlcdbqi 320 6 having have VBG cord-104239-xxlcdbqi 320 7 proximity proximity NN cord-104239-xxlcdbqi 320 8 to to IN cord-104239-xxlcdbqi 320 9 a a DT cord-104239-xxlcdbqi 320 10 second second JJ cord-104239-xxlcdbqi 320 11 bud bud NN cord-104239-xxlcdbqi 320 12 in in IN cord-104239-xxlcdbqi 320 13 the the DT cord-104239-xxlcdbqi 320 14 cell cell NN cord-104239-xxlcdbqi 320 15 . . . cord-104239-xxlcdbqi 321 1 Randomly randomly RB cord-104239-xxlcdbqi 321 2 chosen choose VBN cord-104239-xxlcdbqi 321 3 ER ER NNP cord-104239-xxlcdbqi 321 4 buds bud NNS cord-104239-xxlcdbqi 321 5 present present JJ cord-104239-xxlcdbqi 321 6 in in IN cord-104239-xxlcdbqi 321 7 RBL rbl NN cord-104239-xxlcdbqi 321 8 cells cell NNS cord-104239-xxlcdbqi 321 9 were be VBD cord-104239-xxlcdbqi 321 10 assigned assign VBN cord-104239-xxlcdbqi 321 11 as as IN cord-104239-xxlcdbqi 321 12 the the DT cord-104239-xxlcdbqi 321 13 center center NN cord-104239-xxlcdbqi 321 14 of of IN cord-104239-xxlcdbqi 321 15 reference reference NN cord-104239-xxlcdbqi 321 16 , , , cord-104239-xxlcdbqi 321 17 and and CC cord-104239-xxlcdbqi 321 18 distances distance NNS cord-104239-xxlcdbqi 321 19 between between IN cord-104239-xxlcdbqi 321 20 it -PRON- PRP cord-104239-xxlcdbqi 321 21 and and CC cord-104239-xxlcdbqi 321 22 any any DT cord-104239-xxlcdbqi 321 23 other other JJ cord-104239-xxlcdbqi 321 24 buds bud NNS cord-104239-xxlcdbqi 321 25 present present JJ cord-104239-xxlcdbqi 321 26 in in IN cord-104239-xxlcdbqi 321 27 30 30 CD cord-104239-xxlcdbqi 321 28 consecutive consecutive JJ cord-104239-xxlcdbqi 321 29 serial serial JJ cord-104239-xxlcdbqi 321 30 sections section NNS cord-104239-xxlcdbqi 321 31 were be VBD cord-104239-xxlcdbqi 321 32 determined determine VBN cord-104239-xxlcdbqi 321 33 by by IN cord-104239-xxlcdbqi 321 34 building build VBG cord-104239-xxlcdbqi 321 35 a a DT cord-104239-xxlcdbqi 321 36 series series NN cord-104239-xxlcdbqi 321 37 of of IN cord-104239-xxlcdbqi 321 38 concentric concentric JJ cord-104239-xxlcdbqi 321 39 shells shell NNS cord-104239-xxlcdbqi 321 40 with with IN cord-104239-xxlcdbqi 321 41 a a DT cord-104239-xxlcdbqi 321 42 constant constant JJ cord-104239-xxlcdbqi 321 43 volume volume NN cord-104239-xxlcdbqi 321 44 of of IN cord-104239-xxlcdbqi 321 45 0.0042 0.0042 CD cord-104239-xxlcdbqi 321 46 ixm ixm NN cord-104239-xxlcdbqi 321 47 3 3 CD cord-104239-xxlcdbqi 321 48 , , , cord-104239-xxlcdbqi 321 49 corresponding correspond VBG cord-104239-xxlcdbqi 321 50 to to IN cord-104239-xxlcdbqi 321 51 the the DT cord-104239-xxlcdbqi 321 52 volume volume NN cord-104239-xxlcdbqi 321 53 of of IN cord-104239-xxlcdbqi 321 54 the the DT cord-104239-xxlcdbqi 321 55 first first JJ cord-104239-xxlcdbqi 321 56 internal internal JJ cord-104239-xxlcdbqi 321 57 shell shell NN cord-104239-xxlcdbqi 321 58 having have VBG cord-104239-xxlcdbqi 321 59 a a DT cord-104239-xxlcdbqi 321 60 diameter diameter NN cord-104239-xxlcdbqi 321 61 0.2 0.2 CD cord-104239-xxlcdbqi 321 62 Ixm Ixm NNP cord-104239-xxlcdbqi 321 63 ( ( -LRB- cord-104239-xxlcdbqi 321 64 to to TO cord-104239-xxlcdbqi 321 65 encompass encompass VB cord-104239-xxlcdbqi 321 66 a a DT cord-104239-xxlcdbqi 321 67 single single JJ cord-104239-xxlcdbqi 321 68 bud bud NN cord-104239-xxlcdbqi 321 69 ) ) -RRB- cord-104239-xxlcdbqi 321 70 as as IN cord-104239-xxlcdbqi 321 71 described describe VBN cord-104239-xxlcdbqi 321 72 in in IN cord-104239-xxlcdbqi 321 73 the the DT cord-104239-xxlcdbqi 321 74 Materials Materials NNPS cord-104239-xxlcdbqi 321 75 and and CC cord-104239-xxlcdbqi 321 76 Methods method NNS cord-104239-xxlcdbqi 321 77 . . . cord-104239-xxlcdbqi 322 1 The the DT cord-104239-xxlcdbqi 322 2 probability probability NN cord-104239-xxlcdbqi 322 3 was be VBD cord-104239-xxlcdbqi 322 4 determined determine VBN cord-104239-xxlcdbqi 322 5 by by IN cord-104239-xxlcdbqi 322 6 counting count VBG cord-104239-xxlcdbqi 322 7 the the DT cord-104239-xxlcdbqi 322 8 number number NN cord-104239-xxlcdbqi 322 9 of of IN cord-104239-xxlcdbqi 322 10 buds bud NNS cord-104239-xxlcdbqi 322 11 detected detect VBN cord-104239-xxlcdbqi 322 12 in in IN cord-104239-xxlcdbqi 322 13 each each DT cord-104239-xxlcdbqi 322 14 shell shell NN cord-104239-xxlcdbqi 322 15 relative relative NN cord-104239-xxlcdbqi 322 16 to to IN cord-104239-xxlcdbqi 322 17 the the DT cord-104239-xxlcdbqi 322 18 total total JJ cord-104239-xxlcdbqi 322 19 number number NN cord-104239-xxlcdbqi 322 20 of of IN cord-104239-xxlcdbqi 322 21 buds bud NNS cord-104239-xxlcdbqi 322 22 detected detect VBN cord-104239-xxlcdbqi 322 23 ( ( -LRB- cord-104239-xxlcdbqi 322 24 percentage percentage NN cord-104239-xxlcdbqi 322 25 of of IN cord-104239-xxlcdbqi 322 26 total total NN cord-104239-xxlcdbqi 322 27 ) ) -RRB- cord-104239-xxlcdbqi 322 28 . . . cord-104239-xxlcdbqi 323 1 This this DT cord-104239-xxlcdbqi 323 2 value value NN cord-104239-xxlcdbqi 323 3 is be VBZ cord-104239-xxlcdbqi 323 4 plotted plot VBN cord-104239-xxlcdbqi 323 5 as as IN cord-104239-xxlcdbqi 323 6 shell shell NN cord-104239-xxlcdbqi 323 7 number number NN cord-104239-xxlcdbqi 323 8 in in IN cord-104239-xxlcdbqi 323 9 which which WDT cord-104239-xxlcdbqi 323 10 a a DT cord-104239-xxlcdbqi 323 11 second second JJ cord-104239-xxlcdbqi 323 12 bud bud NN cord-104239-xxlcdbqi 323 13 was be VBD cord-104239-xxlcdbqi 323 14 found find VBN cord-104239-xxlcdbqi 323 15 ( ( -LRB- cord-104239-xxlcdbqi 323 16 open open JJ cord-104239-xxlcdbqi 323 17 circles circle NNS cord-104239-xxlcdbqi 323 18 ) ) -RRB- cord-104239-xxlcdbqi 323 19 or or CC cord-104239-xxlcdbqi 323 20 relative relative JJ cord-104239-xxlcdbqi 323 21 to to IN cord-104239-xxlcdbqi 323 22 the the DT cord-104239-xxlcdbqi 323 23 outermost outermost JJS cord-104239-xxlcdbqi 323 24 diameter diameter NN cord-104239-xxlcdbqi 323 25 of of IN cord-104239-xxlcdbqi 323 26 a a DT cord-104239-xxlcdbqi 323 27 particular particular JJ cord-104239-xxlcdbqi 323 28 shell shell NN cord-104239-xxlcdbqi 323 29 ( ( -LRB- cord-104239-xxlcdbqi 323 30 closed closed JJ cord-104239-xxlcdbqi 323 31 circles circle NNS cord-104239-xxlcdbqi 323 32 ) ) -RRB- cord-104239-xxlcdbqi 323 33 . . . cord-104239-xxlcdbqi 324 1 The the DT cord-104239-xxlcdbqi 324 2 calculated calculated JJ cord-104239-xxlcdbqi 324 3 probability probability NN cord-104239-xxlcdbqi 324 4 of of IN cord-104239-xxlcdbqi 324 5 a a DT cord-104239-xxlcdbqi 324 6 second second JJ cord-104239-xxlcdbqi 324 7 bud bud NN cord-104239-xxlcdbqi 324 8 having have VBG cord-104239-xxlcdbqi 324 9 a a DT cord-104239-xxlcdbqi 324 10 completely completely RB cord-104239-xxlcdbqi 324 11 random random JJ cord-104239-xxlcdbqi 324 12 distribution distribution NN cord-104239-xxlcdbqi 324 13 in in IN cord-104239-xxlcdbqi 324 14 the the DT cord-104239-xxlcdbqi 324 15 cell cell NN cord-104239-xxlcdbqi 324 16 ( ( -LRB- cord-104239-xxlcdbqi 324 17 0.18 0.18 CD cord-104239-xxlcdbqi 324 18 % % NN cord-104239-xxlcdbqi 324 19 ) ) -RRB- cord-104239-xxlcdbqi 324 20 is be VBZ cord-104239-xxlcdbqi 324 21 presented present VBN cord-104239-xxlcdbqi 324 22 for for IN cord-104239-xxlcdbqi 324 23 comparison comparison NN cord-104239-xxlcdbqi 324 24 ( ( -LRB- cord-104239-xxlcdbqi 324 25 diamonds diamond NNS cord-104239-xxlcdbqi 324 26 ) ) -RRB- cord-104239-xxlcdbqi 324 27 . . . cord-104239-xxlcdbqi 325 1 in in IN cord-104239-xxlcdbqi 325 2 the the DT cord-104239-xxlcdbqi 325 3 absence absence NN cord-104239-xxlcdbqi 325 4 of of IN cord-104239-xxlcdbqi 325 5 GTP gtp NN cord-104239-xxlcdbqi 325 6 hydrolysis hydrolysis NN cord-104239-xxlcdbqi 325 7 , , , cord-104239-xxlcdbqi 325 8 but but CC cord-104239-xxlcdbqi 325 9 that that IN cord-104239-xxlcdbqi 325 10 the the DT cord-104239-xxlcdbqi 325 11 vesicles vesicle NNS cord-104239-xxlcdbqi 325 12 fail fail VBP cord-104239-xxlcdbqi 325 13 to to TO cord-104239-xxlcdbqi 325 14 complete complete VB cord-104239-xxlcdbqi 325 15 separation separation NN cord-104239-xxlcdbqi 325 16 from from IN cord-104239-xxlcdbqi 325 17 one one CD cord-104239-xxlcdbqi 325 18 another another DT cord-104239-xxlcdbqi 325 19 . . . cord-104239-xxlcdbqi 326 1 Upon upon IN cord-104239-xxlcdbqi 326 2 immunolabeling immunolabele VBG cord-104239-xxlcdbqi 326 3 , , , cord-104239-xxlcdbqi 326 4 we -PRON- PRP cord-104239-xxlcdbqi 326 5 found find VBD cord-104239-xxlcdbqi 326 6 accumulated accumulate VBN cord-104239-xxlcdbqi 326 7 vesicles vesicle NNS cord-104239-xxlcdbqi 326 8 to to TO cord-104239-xxlcdbqi 326 9 be be VB cord-104239-xxlcdbqi 326 10 substantially substantially RB cord-104239-xxlcdbqi 326 11 enriched enrich VBN cord-104239-xxlcdbqi 326 12 in in IN cord-104239-xxlcdbqi 326 13 VSV VSV NNP cord-104239-xxlcdbqi 326 14 - - HYPH cord-104239-xxlcdbqi 326 15 G G NNP cord-104239-xxlcdbqi 326 16 ( ( -LRB- cord-104239-xxlcdbqi 326 17 Pind Pind NNP cord-104239-xxlcdbqi 326 18 et et FW cord-104239-xxlcdbqi 326 19 al al NNP cord-104239-xxlcdbqi 326 20 . . NNP cord-104239-xxlcdbqi 326 21 , , , cord-104239-xxlcdbqi 326 22 1994a 1994a CD cord-104239-xxlcdbqi 326 23 ) ) -RRB- cord-104239-xxlcdbqi 326 24 and and CC cord-104239-xxlcdbqi 326 25 components component NNS cord-104239-xxlcdbqi 326 26 of of IN cord-104239-xxlcdbqi 326 27 both both DT cord-104239-xxlcdbqi 326 28 COPI COPI NNP cord-104239-xxlcdbqi 326 29 ( ( -LRB- cord-104239-xxlcdbqi 326 30 13-COP 13-cop CD cord-104239-xxlcdbqi 326 31 ) ) -RRB- cord-104239-xxlcdbqi 327 1 ( ( -LRB- cord-104239-xxlcdbqi 327 2 Pind Pind NNP cord-104239-xxlcdbqi 327 3 et et FW cord-104239-xxlcdbqi 327 4 al al NNP cord-104239-xxlcdbqi 327 5 . . . cord-104239-xxlcdbqi 328 1 , , , cord-104239-xxlcdbqi 328 2 1994a 1994a CD cord-104239-xxlcdbqi 328 3 ; ; : cord-104239-xxlcdbqi 328 4 Griffiths Griffiths NNP cord-104239-xxlcdbqi 328 5 et et NNP cord-104239-xxlcdbqi 328 6 al al NNP cord-104239-xxlcdbqi 328 7 . . NNP cord-104239-xxlcdbqi 328 8 , , , cord-104239-xxlcdbqi 328 9 1995b 1995b CD cord-104239-xxlcdbqi 328 10 ) ) -RRB- cord-104239-xxlcdbqi 328 11 and and CC cord-104239-xxlcdbqi 328 12 COPII COPII NNP cord-104239-xxlcdbqi 328 13 coats coat NNS cord-104239-xxlcdbqi 328 14 ( ( -LRB- cord-104239-xxlcdbqi 328 15 Sec13 Sec13 NNP cord-104239-xxlcdbqi 328 16 and and CC cord-104239-xxlcdbqi 328 17 Sec Sec NNP cord-104239-xxlcdbqi 328 18 23 23 CD cord-104239-xxlcdbqi 328 19 ) ) -RRB- cord-104239-xxlcdbqi 329 1 ( ( -LRB- cord-104239-xxlcdbqi 329 2 Fig Fig NNP cord-104239-xxlcdbqi 329 3 . . NNP cord-104239-xxlcdbqi 329 4 8 8 CD cord-104239-xxlcdbqi 329 5 , , , cord-104239-xxlcdbqi 329 6 A A NNP cord-104239-xxlcdbqi 329 7 - - HYPH cord-104239-xxlcdbqi 329 8 C C NNP cord-104239-xxlcdbqi 329 9 ) ) -RRB- cord-104239-xxlcdbqi 329 10 . . . cord-104239-xxlcdbqi 330 1 To to TO cord-104239-xxlcdbqi 330 2 establish establish VB cord-104239-xxlcdbqi 330 3 that that IN cord-104239-xxlcdbqi 330 4 necklaces necklace NNS cord-104239-xxlcdbqi 330 5 formed form VBN cord-104239-xxlcdbqi 330 6 in in IN cord-104239-xxlcdbqi 330 7 response response NN cord-104239-xxlcdbqi 330 8 to to IN cord-104239-xxlcdbqi 330 9 a a DT cord-104239-xxlcdbqi 330 10 specific specific JJ cord-104239-xxlcdbqi 330 11 block block NN cord-104239-xxlcdbqi 330 12 in in IN cord-104239-xxlcdbqi 330 13 COPII COPII NNP cord-104239-xxlcdbqi 330 14 coat coat NN cord-104239-xxlcdbqi 330 15 disassembly disassembly NN cord-104239-xxlcdbqi 330 16 , , , cord-104239-xxlcdbqi 330 17 we -PRON- PRP cord-104239-xxlcdbqi 330 18 examined examine VBD cord-104239-xxlcdbqi 330 19 the the DT cord-104239-xxlcdbqi 330 20 ef ef NNP cord-104239-xxlcdbqi 330 21 - - HYPH cord-104239-xxlcdbqi 330 22 fect fect NN cord-104239-xxlcdbqi 330 23 of of IN cord-104239-xxlcdbqi 330 24 an an DT cord-104239-xxlcdbqi 330 25 activated activate VBN cord-104239-xxlcdbqi 330 26 , , , cord-104239-xxlcdbqi 330 27 GTP GTP NNP cord-104239-xxlcdbqi 330 28 - - HYPH cord-104239-xxlcdbqi 330 29 restricted restrict VBN cord-104239-xxlcdbqi 330 30 mutant mutant NN cord-104239-xxlcdbqi 330 31 of of IN cord-104239-xxlcdbqi 330 32 Sarl Sarl NNP cord-104239-xxlcdbqi 330 33 , , , cord-104239-xxlcdbqi 330 34 Sarl[H79 Sarl[H79 NNP cord-104239-xxlcdbqi 330 35 G G NNP cord-104239-xxlcdbqi 330 36 ] ] -RRB- cord-104239-xxlcdbqi 330 37 , , , cord-104239-xxlcdbqi 330 38 which which WDT cord-104239-xxlcdbqi 330 39 promotes promote VBZ cord-104239-xxlcdbqi 330 40 vesicle vesicle NN cord-104239-xxlcdbqi 330 41 accumulation accumulation NN cord-104239-xxlcdbqi 330 42 in in IN cord-104239-xxlcdbqi 330 43 vivo vivo NN cord-104239-xxlcdbqi 330 44 and and CC cord-104239-xxlcdbqi 330 45 in in IN cord-104239-xxlcdbqi 330 46 vitro vitro FW cord-104239-xxlcdbqi 331 1 ( ( -LRB- cord-104239-xxlcdbqi 331 2 Aridor Aridor NNP cord-104239-xxlcdbqi 331 3 et et NNP cord-104239-xxlcdbqi 331 4 al al NNP cord-104239-xxlcdbqi 331 5 . . NNP cord-104239-xxlcdbqi 331 6 , , , cord-104239-xxlcdbqi 331 7 1995 1995 CD cord-104239-xxlcdbqi 331 8 ; ; : cord-104239-xxlcdbqi 331 9 Kuge Kuge NNP cord-104239-xxlcdbqi 331 10 et et NNP cord-104239-xxlcdbqi 331 11 al al NNP cord-104239-xxlcdbqi 331 12 . . NNP cord-104239-xxlcdbqi 331 13 , , , cord-104239-xxlcdbqi 331 14 1994 1994 CD cord-104239-xxlcdbqi 331 15 ) ) -RRB- cord-104239-xxlcdbqi 331 16 . . . cord-104239-xxlcdbqi 332 1 Examination examination NN cord-104239-xxlcdbqi 332 2 using use VBG cord-104239-xxlcdbqi 332 3 strong strong JJ cord-104239-xxlcdbqi 332 4 fixation fixation NN cord-104239-xxlcdbqi 332 5 conditions condition NNS cord-104239-xxlcdbqi 332 6 revealed reveal VBD cord-104239-xxlcdbqi 332 7 a a DT cord-104239-xxlcdbqi 332 8 striking striking JJ cord-104239-xxlcdbqi 332 9 similarity similarity NN cord-104239-xxlcdbqi 332 10 to to IN cord-104239-xxlcdbqi 332 11 GTP~/S gtp~/s NN cord-104239-xxlcdbqi 332 12 - - JJ cord-104239-xxlcdbqi 332 13 formed form VBN cord-104239-xxlcdbqi 332 14 structures structure NNS cord-104239-xxlcdbqi 332 15 . . . cord-104239-xxlcdbqi 333 1 Vesicles vesicle NNS cord-104239-xxlcdbqi 333 2 were be VBD cord-104239-xxlcdbqi 333 3 detected detect VBN cord-104239-xxlcdbqi 333 4 as as IN cord-104239-xxlcdbqi 333 5 both both DT cord-104239-xxlcdbqi 333 6 clusters cluster NNS cord-104239-xxlcdbqi 333 7 ( ( -LRB- cord-104239-xxlcdbqi 333 8 Fig Fig NNP cord-104239-xxlcdbqi 333 9 . . . cord-104239-xxlcdbqi 333 10 7 7 CD cord-104239-xxlcdbqi 333 11 D d NN cord-104239-xxlcdbqi 333 12 ) ) -RRB- cord-104239-xxlcdbqi 333 13 or or CC cord-104239-xxlcdbqi 333 14 as as IN cord-104239-xxlcdbqi 333 15 necklaces necklace NNS cord-104239-xxlcdbqi 334 1 ( ( -LRB- cord-104239-xxlcdbqi 334 2 Fig Fig NNP cord-104239-xxlcdbqi 334 3 . . . cord-104239-xxlcdbqi 334 4 8 8 CD cord-104239-xxlcdbqi 334 5 E e NN cord-104239-xxlcdbqi 334 6 ) ) -RRB- cord-104239-xxlcdbqi 334 7 emerging emerge VBG cord-104239-xxlcdbqi 334 8 from from IN cord-104239-xxlcdbqi 334 9 restricted restricted JJ cord-104239-xxlcdbqi 334 10 regions region NNS cord-104239-xxlcdbqi 334 11 of of IN cord-104239-xxlcdbqi 334 12 the the DT cord-104239-xxlcdbqi 334 13 ER ER NNP cord-104239-xxlcdbqi 334 14 . . . cord-104239-xxlcdbqi 335 1 As as IN cord-104239-xxlcdbqi 335 2 expected expect VBN cord-104239-xxlcdbqi 335 3 , , , cord-104239-xxlcdbqi 335 4 coats coat NNS cord-104239-xxlcdbqi 335 5 were be VBD cord-104239-xxlcdbqi 335 6 enriched enrich VBN cord-104239-xxlcdbqi 335 7 in in IN cord-104239-xxlcdbqi 335 8 the the DT cord-104239-xxlcdbqi 335 9 COPII COPII NNP cord-104239-xxlcdbqi 335 10 components component NNS cord-104239-xxlcdbqi 335 11 Secl3p Secl3p NNP cord-104239-xxlcdbqi 335 12 ( ( -LRB- cord-104239-xxlcdbqi 335 13 arrowheads arrowhead NNS cord-104239-xxlcdbqi 335 14 in in IN cord-104239-xxlcdbqi 335 15 A A NNP cord-104239-xxlcdbqi 335 16 , , , cord-104239-xxlcdbqi 335 17 C C NNP cord-104239-xxlcdbqi 335 18 , , , cord-104239-xxlcdbqi 335 19 and and CC cord-104239-xxlcdbqi 335 20 D D NNP cord-104239-xxlcdbqi 335 21 ) ) -RRB- cord-104239-xxlcdbqi 335 22 and and CC cord-104239-xxlcdbqi 335 23 Sec23p Sec23p NNP cord-104239-xxlcdbqi 335 24 ( ( -LRB- cord-104239-xxlcdbqi 335 25 arrowheads arrowhead NNS cord-104239-xxlcdbqi 335 26 in in IN cord-104239-xxlcdbqi 335 27 B b NN cord-104239-xxlcdbqi 335 28 , , , cord-104239-xxlcdbqi 335 29 and and CC cord-104239-xxlcdbqi 335 30 E e LS cord-104239-xxlcdbqi 335 31 ) ) -RRB- cord-104239-xxlcdbqi 335 32 as as IN cord-104239-xxlcdbqi 335 33 shown show VBN cord-104239-xxlcdbqi 335 34 by by IN cord-104239-xxlcdbqi 335 35 the the DT cord-104239-xxlcdbqi 335 36 distribution distribution NN cord-104239-xxlcdbqi 335 37 of of IN cord-104239-xxlcdbqi 335 38 gold gold NN cord-104239-xxlcdbqi 335 39 particles particle NNS cord-104239-xxlcdbqi 335 40 using use VBG cord-104239-xxlcdbqi 335 41 specific specific JJ cord-104239-xxlcdbqi 335 42 antibodies antibody NNS cord-104239-xxlcdbqi 335 43 . . . cord-104239-xxlcdbqi 336 1 Note note VB cord-104239-xxlcdbqi 336 2 that that IN cord-104239-xxlcdbqi 336 3 the the DT cord-104239-xxlcdbqi 336 4 cisternal cisternal JJ cord-104239-xxlcdbqi 336 5 portion portion NN cord-104239-xxlcdbqi 336 6 of of IN cord-104239-xxlcdbqi 336 7 the the DT cord-104239-xxlcdbqi 336 8 Golgi Golgi NNP cord-104239-xxlcdbqi 336 9 apparatus apparatus NN cord-104239-xxlcdbqi 336 10 with with IN cord-104239-xxlcdbqi 336 11 the the DT cord-104239-xxlcdbqi 336 12 trans trans NNP cord-104239-xxlcdbqi 336 13 face face NN cord-104239-xxlcdbqi 336 14 labeled label VBN cord-104239-xxlcdbqi 336 15 in in IN cord-104239-xxlcdbqi 336 16 C C NNP cord-104239-xxlcdbqi 336 17 and and CC cord-104239-xxlcdbqi 336 18 F F NNP cord-104239-xxlcdbqi 336 19 remains remain VBZ cord-104239-xxlcdbqi 336 20 unlabeled unlabeled JJ cord-104239-xxlcdbqi 336 21 by by IN cord-104239-xxlcdbqi 336 22 Sec13p Sec13p NNP cord-104239-xxlcdbqi 336 23 - - HYPH cord-104239-xxlcdbqi 336 24 and and CC cord-104239-xxlcdbqi 336 25 Sec23p Sec23p NNP cord-104239-xxlcdbqi 336 26 - - HYPH cord-104239-xxlcdbqi 336 27 specific specific JJ cord-104239-xxlcdbqi 336 28 antibodies antibody NNS cord-104239-xxlcdbqi 336 29 , , , cord-104239-xxlcdbqi 336 30 whereas whereas IN cord-104239-xxlcdbqi 336 31 clusters cluster NNS cord-104239-xxlcdbqi 336 32 ( ( -LRB- cord-104239-xxlcdbqi 336 33 arrows arrow NNS cord-104239-xxlcdbqi 336 34 ) ) -RRB- cord-104239-xxlcdbqi 336 35 that that WDT cord-104239-xxlcdbqi 336 36 are be VBP cord-104239-xxlcdbqi 336 37 closely closely RB cord-104239-xxlcdbqi 336 38 adjacent adjacent JJ cord-104239-xxlcdbqi 336 39 to to IN cord-104239-xxlcdbqi 336 40 the the DT cord-104239-xxlcdbqi 336 41 Golgi Golgi NNP cord-104239-xxlcdbqi 336 42 complex complex NN cord-104239-xxlcdbqi 336 43 contain contain VBP cord-104239-xxlcdbqi 336 44 both both DT cord-104239-xxlcdbqi 336 45 Secl3p Secl3p NNS cord-104239-xxlcdbqi 336 46 and and CC cord-104239-xxlcdbqi 336 47 Sec23p Sec23p NNP cord-104239-xxlcdbqi 336 48 ( ( -LRB- cord-104239-xxlcdbqi 336 49 C C NNP cord-104239-xxlcdbqi 336 50 and and CC cord-104239-xxlcdbqi 336 51 F F NNP cord-104239-xxlcdbqi 336 52 ) ) -RRB- cord-104239-xxlcdbqi 336 53 . . . cord-104239-xxlcdbqi 337 1 Bar bar NN cord-104239-xxlcdbqi 337 2 , , , cord-104239-xxlcdbqi 337 3 0.05 0.05 CD cord-104239-xxlcdbqi 337 4 txm txm NNP cord-104239-xxlcdbqi 337 5 . . . cord-104239-xxlcdbqi 338 1 Bannykh Bannykh NNP cord-104239-xxlcdbqi 338 2 et et NNP cord-104239-xxlcdbqi 338 3 al al NNP cord-104239-xxlcdbqi 338 4 . . . cord-104239-xxlcdbqi 339 1 Organization Organization NNP cord-104239-xxlcdbqi 339 2 of of IN cord-104239-xxlcdbqi 339 3 ER ER NNP cord-104239-xxlcdbqi 339 4 Export Export NNP cord-104239-xxlcdbqi 339 5 Complexes Complexes NNP cord-104239-xxlcdbqi 340 1 ( ( -LRB- cord-104239-xxlcdbqi 340 2 Fig Fig NNP cord-104239-xxlcdbqi 340 3 . . . cord-104239-xxlcdbqi 340 4 8 8 CD cord-104239-xxlcdbqi 340 5 E e NN cord-104239-xxlcdbqi 340 6 ) ) -RRB- cord-104239-xxlcdbqi 340 7 and and CC cord-104239-xxlcdbqi 340 8 Sec23p Sec23p NNP cord-104239-xxlcdbqi 340 9 ( ( -LRB- cord-104239-xxlcdbqi 340 10 Fig Fig NNP cord-104239-xxlcdbqi 340 11 . . . cord-104239-xxlcdbqi 340 12 8 8 CD cord-104239-xxlcdbqi 340 13 F f NN cord-104239-xxlcdbqi 340 14 ) ) -RRB- cord-104239-xxlcdbqi 340 15 . . . cord-104239-xxlcdbqi 341 1 No no DT cord-104239-xxlcdbqi 341 2 COPI COPI NNP cord-104239-xxlcdbqi 341 3 staining staining NN cord-104239-xxlcdbqi 341 4 could could MD cord-104239-xxlcdbqi 341 5 be be VB cord-104239-xxlcdbqi 341 6 detected detect VBN cord-104239-xxlcdbqi 341 7 using use VBG cord-104239-xxlcdbqi 341 8 an an DT cord-104239-xxlcdbqi 341 9 antibody antibody NN cord-104239-xxlcdbqi 341 10 specific specific JJ cord-104239-xxlcdbqi 341 11 for for IN cord-104239-xxlcdbqi 341 12 13-COP 13-cop CD cord-104239-xxlcdbqi 341 13 in in IN cord-104239-xxlcdbqi 341 14 these these DT cord-104239-xxlcdbqi 341 15 structures structure NNS cord-104239-xxlcdbqi 341 16 ( ( -LRB- cord-104239-xxlcdbqi 341 17 not not RB cord-104239-xxlcdbqi 341 18 shown show VBN cord-104239-xxlcdbqi 341 19 ) ) -RRB- cord-104239-xxlcdbqi 342 1 ( ( -LRB- cord-104239-xxlcdbqi 342 2 Aridor Aridor NNP cord-104239-xxlcdbqi 342 3 et et NNP cord-104239-xxlcdbqi 342 4 al al NNP cord-104239-xxlcdbqi 342 5 . . NNP cord-104239-xxlcdbqi 342 6 , , , cord-104239-xxlcdbqi 342 7 1995 1995 CD cord-104239-xxlcdbqi 342 8 ) ) -RRB- cord-104239-xxlcdbqi 342 9 . . . cord-104239-xxlcdbqi 343 1 To to TO cord-104239-xxlcdbqi 343 2 examine examine VB cord-104239-xxlcdbqi 343 3 the the DT cord-104239-xxlcdbqi 343 4 role role NN cord-104239-xxlcdbqi 343 5 of of IN cord-104239-xxlcdbqi 343 6 COPII COPII NNP cord-104239-xxlcdbqi 343 7 in in IN cord-104239-xxlcdbqi 343 8 VSV VSV NNP cord-104239-xxlcdbqi 343 9 - - HYPH cord-104239-xxlcdbqi 343 10 G G NNP cord-104239-xxlcdbqi 343 11 concentration concentration NN cord-104239-xxlcdbqi 343 12 and and CC cord-104239-xxlcdbqi 343 13 the the DT cord-104239-xxlcdbqi 343 14 appearance appearance NN cord-104239-xxlcdbqi 343 15 of of IN cord-104239-xxlcdbqi 343 16 VTCs vtc NNS cord-104239-xxlcdbqi 343 17 in in IN cord-104239-xxlcdbqi 343 18 export export NN cord-104239-xxlcdbqi 343 19 complexes complex NNS cord-104239-xxlcdbqi 343 20 , , , cord-104239-xxlcdbqi 343 21 we -PRON- PRP cord-104239-xxlcdbqi 343 22 used use VBD cord-104239-xxlcdbqi 343 23 semi semi JJ cord-104239-xxlcdbqi 343 24 - - JJ cord-104239-xxlcdbqi 343 25 intact intact JJ cord-104239-xxlcdbqi 343 26 cells cell NNS cord-104239-xxlcdbqi 343 27 infected infect VBN cord-104239-xxlcdbqi 343 28 with with IN cord-104239-xxlcdbqi 343 29 a a DT cord-104239-xxlcdbqi 343 30 temperature temperature NN cord-104239-xxlcdbqi 343 31 - - HYPH cord-104239-xxlcdbqi 343 32 sensitive sensitive JJ cord-104239-xxlcdbqi 343 33 form form NN cord-104239-xxlcdbqi 343 34 of of IN cord-104239-xxlcdbqi 343 35 VSV VSV NNP cord-104239-xxlcdbqi 343 36 - - HYPH cord-104239-xxlcdbqi 343 37 G G NNP cord-104239-xxlcdbqi 343 38 whose whose WP$ cord-104239-xxlcdbqi 343 39 transport transport NN cord-104239-xxlcdbqi 343 40 is be VBZ cord-104239-xxlcdbqi 343 41 blocked block VBN cord-104239-xxlcdbqi 343 42 at at IN cord-104239-xxlcdbqi 343 43 the the DT cord-104239-xxlcdbqi 343 44 restrictive restrictive JJ cord-104239-xxlcdbqi 343 45 temperature temperature NN cord-104239-xxlcdbqi 343 46 ( ( -LRB- cord-104239-xxlcdbqi 343 47 39.5 39.5 CD cord-104239-xxlcdbqi 343 48 ° ° NN cord-104239-xxlcdbqi 343 49 C c NN cord-104239-xxlcdbqi 343 50 ) ) -RRB- cord-104239-xxlcdbqi 344 1 ( ( -LRB- cord-104239-xxlcdbqi 344 2 Lafay Lafay NNP cord-104239-xxlcdbqi 344 3 , , , cord-104239-xxlcdbqi 344 4 1974 1974 CD cord-104239-xxlcdbqi 344 5 ; ; : cord-104239-xxlcdbqi 344 6 Plutner Plutner NNP cord-104239-xxlcdbqi 344 7 et et NNP cord-104239-xxlcdbqi 344 8 al al NNP cord-104239-xxlcdbqi 344 9 . . NNP cord-104239-xxlcdbqi 344 10 , , , cord-104239-xxlcdbqi 344 11 1992 1992 CD cord-104239-xxlcdbqi 344 12 ) ) -RRB- cord-104239-xxlcdbqi 344 13 . . . cord-104239-xxlcdbqi 345 1 Transfer transfer NN cord-104239-xxlcdbqi 345 2 of of IN cord-104239-xxlcdbqi 345 3 cells cell NNS cord-104239-xxlcdbqi 345 4 to to IN cord-104239-xxlcdbqi 345 5 the the DT cord-104239-xxlcdbqi 345 6 permissive permissive JJ cord-104239-xxlcdbqi 345 7 temperature temperature NN cord-104239-xxlcdbqi 345 8 ( ( -LRB- cord-104239-xxlcdbqi 345 9 32 32 CD cord-104239-xxlcdbqi 345 10 ° ° NN cord-104239-xxlcdbqi 345 11 C C NNP cord-104239-xxlcdbqi 345 12 ) ) -RRB- cord-104239-xxlcdbqi 345 13 results result VBZ cord-104239-xxlcdbqi 345 14 in in IN cord-104239-xxlcdbqi 345 15 the the DT cord-104239-xxlcdbqi 345 16 migration migration NN cord-104239-xxlcdbqi 345 17 of of IN cord-104239-xxlcdbqi 345 18 a a DT cord-104239-xxlcdbqi 345 19 synchronous synchronous JJ cord-104239-xxlcdbqi 345 20 wave wave NN cord-104239-xxlcdbqi 345 21 of of IN cord-104239-xxlcdbqi 345 22 ts045 ts045 NNP cord-104239-xxlcdbqi 345 23 VSV VSV NNP cord-104239-xxlcdbqi 345 24 - - HYPH cord-104239-xxlcdbqi 345 25 G G NNP cord-104239-xxlcdbqi 345 26 from from IN cord-104239-xxlcdbqi 345 27 the the DT cord-104239-xxlcdbqi 345 28 ER ER NNP cord-104239-xxlcdbqi 345 29 to to IN cord-104239-xxlcdbqi 345 30 VTCs vtc NNS cord-104239-xxlcdbqi 345 31 and and CC cord-104239-xxlcdbqi 345 32 subsequent subsequent JJ cord-104239-xxlcdbqi 345 33 Golgi Golgi NNP cord-104239-xxlcdbqi 345 34 compartments compartment NNS cord-104239-xxlcdbqi 345 35 ( ( -LRB- cord-104239-xxlcdbqi 345 36 Plutner Plutner NNP cord-104239-xxlcdbqi 345 37 et et NNP cord-104239-xxlcdbqi 345 38 al al NNP cord-104239-xxlcdbqi 345 39 . . NNP cord-104239-xxlcdbqi 345 40 , , , cord-104239-xxlcdbqi 345 41 1992 1992 CD cord-104239-xxlcdbqi 345 42 ; ; : cord-104239-xxlcdbqi 345 43 Balch Balch NNP cord-104239-xxlcdbqi 345 44 et et NNP cord-104239-xxlcdbqi 345 45 al al NNP cord-104239-xxlcdbqi 345 46 . . NNP cord-104239-xxlcdbqi 345 47 , , , cord-104239-xxlcdbqi 345 48 1994 1994 CD cord-104239-xxlcdbqi 345 49 ) ) -RRB- cord-104239-xxlcdbqi 345 50 . . . cord-104239-xxlcdbqi 346 1 After after IN cord-104239-xxlcdbqi 346 2 incubation incubation NN cord-104239-xxlcdbqi 346 3 at at IN cord-104239-xxlcdbqi 346 4 32 32 CD cord-104239-xxlcdbqi 346 5 ° ° NN cord-104239-xxlcdbqi 346 6 C c NN cord-104239-xxlcdbqi 346 7 in in IN cord-104239-xxlcdbqi 346 8 vitro vitro FW cord-104239-xxlcdbqi 346 9 , , , cord-104239-xxlcdbqi 346 10 cells cell NNS cord-104239-xxlcdbqi 346 11 were be VBD cord-104239-xxlcdbqi 346 12 fixed fix VBN cord-104239-xxlcdbqi 346 13 and and CC cord-104239-xxlcdbqi 346 14 stained stain VBN cord-104239-xxlcdbqi 346 15 with with IN cord-104239-xxlcdbqi 346 16 an an DT cord-104239-xxlcdbqi 346 17 antibody antibody NN cord-104239-xxlcdbqi 346 18 specific specific JJ cord-104239-xxlcdbqi 346 19 for for IN cord-104239-xxlcdbqi 346 20 the the DT cord-104239-xxlcdbqi 346 21 cytoplasmic cytoplasmic JJ cord-104239-xxlcdbqi 346 22 tail tail NN cord-104239-xxlcdbqi 346 23 of of IN cord-104239-xxlcdbqi 346 24 VSV VSV NNP cord-104239-xxlcdbqi 346 25 - - HYPH cord-104239-xxlcdbqi 346 26 G G NNP cord-104239-xxlcdbqi 346 27 using use VBG cord-104239-xxlcdbqi 346 28 the the DT cord-104239-xxlcdbqi 346 29 immunodiffusion immunodiffusion NN cord-104239-xxlcdbqi 346 30 protocol protocol NN cord-104239-xxlcdbqi 346 31 . . . cord-104239-xxlcdbqi 347 1 As as IN cord-104239-xxlcdbqi 347 2 shown show VBN cord-104239-xxlcdbqi 347 3 in in IN cord-104239-xxlcdbqi 347 4 Table Table NNP cord-104239-xxlcdbqi 347 5 III III NNP cord-104239-xxlcdbqi 347 6 , , , cord-104239-xxlcdbqi 347 7 incubation incubation NN cord-104239-xxlcdbqi 347 8 in in IN cord-104239-xxlcdbqi 347 9 the the DT cord-104239-xxlcdbqi 347 10 presence presence NN cord-104239-xxlcdbqi 347 11 of of IN cord-104239-xxlcdbqi 347 12 wildtype wildtype JJ cord-104239-xxlcdbqi 347 13 Sarl Sarl NNP cord-104239-xxlcdbqi 347 14 had have VBD cord-104239-xxlcdbqi 347 15 little little JJ cord-104239-xxlcdbqi 347 16 effect effect NN cord-104239-xxlcdbqi 347 17 on on IN cord-104239-xxlcdbqi 347 18 ER ER NNP cord-104239-xxlcdbqi 347 19 export export NN cord-104239-xxlcdbqi 347 20 as as IN cord-104239-xxlcdbqi 347 21 judged judge VBN cord-104239-xxlcdbqi 347 22 by by IN cord-104239-xxlcdbqi 347 23 the the DT cord-104239-xxlcdbqi 347 24 abundance abundance NN cord-104239-xxlcdbqi 347 25 of of IN cord-104239-xxlcdbqi 347 26 VTCs vtc NNS cord-104239-xxlcdbqi 347 27 detectable detectable JJ cord-104239-xxlcdbqi 347 28 in in IN cord-104239-xxlcdbqi 347 29 vitro vitro FW cord-104239-xxlcdbqi 347 30 . . . cord-104239-xxlcdbqi 348 1 In in IN cord-104239-xxlcdbqi 348 2 contrast contrast NN cord-104239-xxlcdbqi 348 3 , , , cord-104239-xxlcdbqi 348 4 the the DT cord-104239-xxlcdbqi 348 5 guanosine guanosine NN cord-104239-xxlcdbqi 348 6 diphosphate diphosphate NN cord-104239-xxlcdbqi 348 7 ( ( -LRB- cord-104239-xxlcdbqi 348 8 GDP)-restricted gdp)-restricte VBN cord-104239-xxlcdbqi 348 9 form form NN cord-104239-xxlcdbqi 348 10 of of IN cord-104239-xxlcdbqi 348 11 Sarl Sarl NNP cord-104239-xxlcdbqi 348 12 ( ( -LRB- cord-104239-xxlcdbqi 348 13 Sarl Sarl NNP cord-104239-xxlcdbqi 348 14 [ [ -LRB- cord-104239-xxlcdbqi 348 15 T39N t39n RB cord-104239-xxlcdbqi 348 16 ] ] -RRB- cord-104239-xxlcdbqi 348 17 ) ) -RRB- cord-104239-xxlcdbqi 348 18 drastically drastically RB cord-104239-xxlcdbqi 348 19 reduced reduce VBD cord-104239-xxlcdbqi 348 20 the the DT cord-104239-xxlcdbqi 348 21 formation formation NN cord-104239-xxlcdbqi 348 22 of of IN cord-104239-xxlcdbqi 348 23 vesicles vesicle NNS cord-104239-xxlcdbqi 348 24 and and CC cord-104239-xxlcdbqi 348 25 VTCs vtc NNS cord-104239-xxlcdbqi 348 26 ( ( -LRB- cord-104239-xxlcdbqi 348 27 Table Table NNP cord-104239-xxlcdbqi 348 28 III III NNP cord-104239-xxlcdbqi 348 29 ) ) -RRB- cord-104239-xxlcdbqi 348 30 , , , cord-104239-xxlcdbqi 348 31 demonstrating demonstrate VBG cord-104239-xxlcdbqi 348 32 the the DT cord-104239-xxlcdbqi 348 33 essential essential JJ cord-104239-xxlcdbqi 348 34 need need NN cord-104239-xxlcdbqi 348 35 to to TO cord-104239-xxlcdbqi 348 36 activate activate VB cord-104239-xxlcdbqi 348 37 Sarl Sarl NNP cord-104239-xxlcdbqi 348 38 to to TO cord-104239-xxlcdbqi 348 39 promote promote VB cord-104239-xxlcdbqi 348 40 membrane membrane NN cord-104239-xxlcdbqi 348 41 flow flow NN cord-104239-xxlcdbqi 348 42 from from IN cord-104239-xxlcdbqi 348 43 the the DT cord-104239-xxlcdbqi 348 44 ER ER NNP cord-104239-xxlcdbqi 348 45 through through IN cord-104239-xxlcdbqi 348 46 export export NN cord-104239-xxlcdbqi 348 47 complexes complex NNS cord-104239-xxlcdbqi 348 48 . . . cord-104239-xxlcdbqi 349 1 In in IN cord-104239-xxlcdbqi 349 2 contrast contrast NN cord-104239-xxlcdbqi 349 3 , , , cord-104239-xxlcdbqi 349 4 incubation incubation NN cord-104239-xxlcdbqi 349 5 in in IN cord-104239-xxlcdbqi 349 6 the the DT cord-104239-xxlcdbqi 349 7 presence presence NN cord-104239-xxlcdbqi 349 8 of of IN cord-104239-xxlcdbqi 349 9 the the DT cord-104239-xxlcdbqi 349 10 GTP GTP NNP cord-104239-xxlcdbqi 349 11 - - HYPH cord-104239-xxlcdbqi 349 12 restricted restrict VBN cord-104239-xxlcdbqi 349 13 mutant mutant NN cord-104239-xxlcdbqi 349 14 ( ( -LRB- cord-104239-xxlcdbqi 349 15 Sarl[H79 Sarl[H79 NNP cord-104239-xxlcdbqi 349 16 G G NNP cord-104239-xxlcdbqi 349 17 ] ] -RRB- cord-104239-xxlcdbqi 349 18 ) ) -RRB- cord-104239-xxlcdbqi 349 19 caused cause VBD cord-104239-xxlcdbqi 349 20 a a DT cord-104239-xxlcdbqi 349 21 dramatic dramatic JJ cord-104239-xxlcdbqi 349 22 accumulation accumulation NN cord-104239-xxlcdbqi 349 23 of of IN cord-104239-xxlcdbqi 349 24 vesicles vesicle NNS cord-104239-xxlcdbqi 349 25 in in IN cord-104239-xxlcdbqi 349 26 clusters cluster NNS cord-104239-xxlcdbqi 349 27 and and CC cord-104239-xxlcdbqi 349 28 an an DT cord-104239-xxlcdbqi 349 29 approximate approximate JJ cord-104239-xxlcdbqi 349 30 twofold twofold JJ cord-104239-xxlcdbqi 349 31 increase increase NN cord-104239-xxlcdbqi 349 32 in in IN cord-104239-xxlcdbqi 349 33 the the DT cord-104239-xxlcdbqi 349 34 apparent apparent JJ cord-104239-xxlcdbqi 349 35 number number NN cord-104239-xxlcdbqi 349 36 of of IN cord-104239-xxlcdbqi 349 37 clusters cluster NNS cord-104239-xxlcdbqi 349 38 per per IN cord-104239-xxlcdbqi 349 39 section section NN cord-104239-xxlcdbqi 349 40 that that WDT cord-104239-xxlcdbqi 349 41 could could MD cord-104239-xxlcdbqi 349 42 be be VB cord-104239-xxlcdbqi 349 43 detected detect VBN cord-104239-xxlcdbqi 349 44 ( ( -LRB- cord-104239-xxlcdbqi 349 45 Table Table NNP cord-104239-xxlcdbqi 349 46 III III NNP cord-104239-xxlcdbqi 349 47 ) ) -RRB- cord-104239-xxlcdbqi 349 48 . . . cord-104239-xxlcdbqi 350 1 When when WRB cord-104239-xxlcdbqi 350 2 we -PRON- PRP cord-104239-xxlcdbqi 350 3 determined determine VBD cord-104239-xxlcdbqi 350 4 the the DT cord-104239-xxlcdbqi 350 5 density density NN cord-104239-xxlcdbqi 350 6 of of IN cord-104239-xxlcdbqi 350 7 VSV VSV NNP cord-104239-xxlcdbqi 350 8 - - HYPH cord-104239-xxlcdbqi 350 9 G G NNP cord-104239-xxlcdbqi 350 10 in in IN cord-104239-xxlcdbqi 350 11 clusters cluster NNS cord-104239-xxlcdbqi 350 12 formed form VBN cord-104239-xxlcdbqi 350 13 in in IN cord-104239-xxlcdbqi 350 14 the the DT cord-104239-xxlcdbqi 350 15 presence presence NN cord-104239-xxlcdbqi 350 16 of of IN cord-104239-xxlcdbqi 350 17 the the DT cord-104239-xxlcdbqi 350 18 Sarl Sarl NNP cord-104239-xxlcdbqi 350 19 - - HYPH cord-104239-xxlcdbqi 350 20 GTP GTP NNP cord-104239-xxlcdbqi 350 21 restricted restrict VBN cord-104239-xxlcdbqi 350 22 mutant mutant NN cord-104239-xxlcdbqi 350 23 , , , cord-104239-xxlcdbqi 350 24 it -PRON- PRP cord-104239-xxlcdbqi 350 25 was be VBD cord-104239-xxlcdbqi 351 1 approximately approximately RB cord-104239-xxlcdbqi 351 2 five five CD cord-104239-xxlcdbqi 351 3 - - : cord-104239-xxlcdbqi 351 4 to to TO cord-104239-xxlcdbqi 351 5 sixfold sixfold VB cord-104239-xxlcdbqi 351 6 higher high JJR cord-104239-xxlcdbqi 351 7 than than IN cord-104239-xxlcdbqi 351 8 that that DT cord-104239-xxlcdbqi 351 9 found find VBN cord-104239-xxlcdbqi 351 10 in in IN cord-104239-xxlcdbqi 351 11 the the DT cord-104239-xxlcdbqi 351 12 ER ER NNP cord-104239-xxlcdbqi 351 13 before before IN cord-104239-xxlcdbqi 351 14 incubation incubation NN cord-104239-xxlcdbqi 351 15 ( ( -LRB- cord-104239-xxlcdbqi 351 16 Table Table NNP cord-104239-xxlcdbqi 351 17 III III NNP cord-104239-xxlcdbqi 351 18 ) ) -RRB- cord-104239-xxlcdbqi 351 19 . . . cord-104239-xxlcdbqi 352 1 This this DT cord-104239-xxlcdbqi 352 2 fold fold NN cord-104239-xxlcdbqi 352 3 - - HYPH cord-104239-xxlcdbqi 352 4 concentration concentration NN cord-104239-xxlcdbqi 352 5 was be VBD cord-104239-xxlcdbqi 352 6 identical identical JJ cord-104239-xxlcdbqi 352 7 to to IN cord-104239-xxlcdbqi 352 8 that that DT cord-104239-xxlcdbqi 352 9 observed observe VBN cord-104239-xxlcdbqi 352 10 in in IN cord-104239-xxlcdbqi 352 11 VTCs vtc NNS cord-104239-xxlcdbqi 352 12 present present JJ cord-104239-xxlcdbqi 352 13 in in IN cord-104239-xxlcdbqi 352 14 control control NN cord-104239-xxlcdbqi 352 15 incubations incubation NNS cord-104239-xxlcdbqi 352 16 lacking lack VBG cord-104239-xxlcdbqi 352 17 inhibitors inhibitor NNS cord-104239-xxlcdbqi 352 18 ( ( -LRB- cord-104239-xxlcdbqi 352 19 Table Table NNP cord-104239-xxlcdbqi 352 20 III III NNP cord-104239-xxlcdbqi 352 21 ) ) -RRB- cord-104239-xxlcdbqi 352 22 . . . cord-104239-xxlcdbqi 353 1 The the DT cord-104239-xxlcdbqi 353 2 five five CD cord-104239-xxlcdbqi 353 3 - - HYPH cord-104239-xxlcdbqi 353 4 to to TO cord-104239-xxlcdbqi 353 5 sixfold sixfold JJ cord-104239-xxlcdbqi 353 6 increase increase NN cord-104239-xxlcdbqi 353 7 in in IN cord-104239-xxlcdbqi 353 8 the the DT cord-104239-xxlcdbqi 353 9 density density NN cord-104239-xxlcdbqi 353 10 of of IN cord-104239-xxlcdbqi 353 11 VSV VSV NNP cord-104239-xxlcdbqi 353 12 - - HYPH cord-104239-xxlcdbqi 353 13 G G NNP cord-104239-xxlcdbqi 353 14 in in IN cord-104239-xxlcdbqi 353 15 vesicles vesicle NNS cord-104239-xxlcdbqi 353 16 that that WDT cord-104239-xxlcdbqi 353 17 accumulate accumulate VBP cord-104239-xxlcdbqi 353 18 in in IN cord-104239-xxlcdbqi 353 19 the the DT cord-104239-xxlcdbqi 353 20 presence presence NN cord-104239-xxlcdbqi 353 21 of of IN cord-104239-xxlcdbqi 353 22 the the DT cord-104239-xxlcdbqi 353 23 mutant mutant NN cord-104239-xxlcdbqi 353 24 demonstrates demonstrate VBZ cord-104239-xxlcdbqi 353 25 that that IN cord-104239-xxlcdbqi 353 26 VSV VSV NNP cord-104239-xxlcdbqi 353 27 - - HYPH cord-104239-xxlcdbqi 353 28 G G NNP cord-104239-xxlcdbqi 353 29 is be VBZ cord-104239-xxlcdbqi 353 30 concentrated concentrate VBN cord-104239-xxlcdbqi 353 31 during during IN cord-104239-xxlcdbqi 353 32 packaging packaging NN cord-104239-xxlcdbqi 353 33 into into IN cord-104239-xxlcdbqi 353 34 COPII COPII NNP cord-104239-xxlcdbqi 353 35 - - HYPH cord-104239-xxlcdbqi 353 36 coated coat VBN cord-104239-xxlcdbqi 353 37 vesicles vesicle NNS cord-104239-xxlcdbqi 353 38 . . . cord-104239-xxlcdbqi 354 1 To to TO cord-104239-xxlcdbqi 354 2 develop develop VB cord-104239-xxlcdbqi 354 3 a a DT cord-104239-xxlcdbqi 354 4 three three CD cord-104239-xxlcdbqi 354 5 - - HYPH cord-104239-xxlcdbqi 354 6 dimensional dimensional JJ cord-104239-xxlcdbqi 354 7 view view NN cord-104239-xxlcdbqi 354 8 of of IN cord-104239-xxlcdbqi 354 9 export export NN cord-104239-xxlcdbqi 354 10 complexes complex NNS cord-104239-xxlcdbqi 354 11 , , , cord-104239-xxlcdbqi 354 12 we -PRON- PRP cord-104239-xxlcdbqi 354 13 applied apply VBD cord-104239-xxlcdbqi 354 14 for for IN cord-104239-xxlcdbqi 354 15 the the DT cord-104239-xxlcdbqi 354 16 first first JJ cord-104239-xxlcdbqi 354 17 time time NN cord-104239-xxlcdbqi 354 18 a a DT cord-104239-xxlcdbqi 354 19 modification modification NN cord-104239-xxlcdbqi 354 20 of of IN cord-104239-xxlcdbqi 354 21 the the DT cord-104239-xxlcdbqi 354 22 quickfreeze quickfreeze NN cord-104239-xxlcdbqi 354 23 , , , cord-104239-xxlcdbqi 354 24 deep deep JJ cord-104239-xxlcdbqi 354 25 - - HYPH cord-104239-xxlcdbqi 354 26 etch etch NN cord-104239-xxlcdbqi 354 27 methodologies methodology NNS cord-104239-xxlcdbqi 354 28 used use VBN cord-104239-xxlcdbqi 354 29 previously previously RB cord-104239-xxlcdbqi 354 30 to to TO cord-104239-xxlcdbqi 354 31 visualize visualize VB cord-104239-xxlcdbqi 354 32 vesicles vesicle NNS cord-104239-xxlcdbqi 354 33 budding bud VBG cord-104239-xxlcdbqi 354 34 from from IN cord-104239-xxlcdbqi 354 35 the the DT cord-104239-xxlcdbqi 354 36 plasma plasma NN cord-104239-xxlcdbqi 354 37 membrane membrane NN cord-104239-xxlcdbqi 354 38 ( ( -LRB- cord-104239-xxlcdbqi 354 39 Heuser Heuser NNP cord-104239-xxlcdbqi 354 40 , , , cord-104239-xxlcdbqi 354 41 1980 1980 CD cord-104239-xxlcdbqi 354 42 ) ) -RRB- cord-104239-xxlcdbqi 354 43 and and CC cord-104239-xxlcdbqi 354 44 Golgi Golgi NNP cord-104239-xxlcdbqi 354 45 compartments compartment NNS cord-104239-xxlcdbqi 354 46 ( ( -LRB- cord-104239-xxlcdbqi 354 47 Weidman Weidman NNP cord-104239-xxlcdbqi 354 48 et et NNP cord-104239-xxlcdbqi 354 49 al al NNP cord-104239-xxlcdbqi 354 50 . . NNP cord-104239-xxlcdbqi 354 51 , , , cord-104239-xxlcdbqi 354 52 1993 1993 CD cord-104239-xxlcdbqi 354 53 ) ) -RRB- cord-104239-xxlcdbqi 354 54 . . . cord-104239-xxlcdbqi 355 1 The the DT cord-104239-xxlcdbqi 355 2 approach approach NN cord-104239-xxlcdbqi 355 3 is be VBZ cord-104239-xxlcdbqi 355 4 particularly particularly RB cord-104239-xxlcdbqi 355 5 applicable applicable JJ cord-104239-xxlcdbqi 355 6 to to IN cord-104239-xxlcdbqi 355 7 semi semi JJ cord-104239-xxlcdbqi 355 8 - - JJ cord-104239-xxlcdbqi 355 9 intact intact JJ cord-104239-xxlcdbqi 355 10 cells cell NNS cord-104239-xxlcdbqi 355 11 where where WRB cord-104239-xxlcdbqi 355 12 the the DT cord-104239-xxlcdbqi 355 13 cytosol cytosol NN cord-104239-xxlcdbqi 355 14 can can MD cord-104239-xxlcdbqi 355 15 be be VB cord-104239-xxlcdbqi 355 16 readily readily RB cord-104239-xxlcdbqi 355 17 washed wash VBN cord-104239-xxlcdbqi 355 18 away away RB cord-104239-xxlcdbqi 355 19 to to TO cord-104239-xxlcdbqi 355 20 reveal reveal VB cord-104239-xxlcdbqi 355 21 structural structural JJ cord-104239-xxlcdbqi 355 22 features feature NNS cord-104239-xxlcdbqi 355 23 of of IN cord-104239-xxlcdbqi 355 24 the the DT cord-104239-xxlcdbqi 355 25 ER ER NNP cord-104239-xxlcdbqi 355 26 membrane membrane NN cord-104239-xxlcdbqi 355 27 surface surface NN cord-104239-xxlcdbqi 355 28 . . . cord-104239-xxlcdbqi 356 1 After after IN cord-104239-xxlcdbqi 356 2 incubation incubation NN cord-104239-xxlcdbqi 356 3 in in IN cord-104239-xxlcdbqi 356 4 vitro vitro FW cord-104239-xxlcdbqi 356 5 in in IN cord-104239-xxlcdbqi 356 6 the the DT cord-104239-xxlcdbqi 356 7 presence presence NN cord-104239-xxlcdbqi 356 8 of of IN cord-104239-xxlcdbqi 356 9 ATP ATP NNP cord-104239-xxlcdbqi 356 10 and and CC cord-104239-xxlcdbqi 356 11 cytosol cytosol NN cord-104239-xxlcdbqi 356 12 , , , cord-104239-xxlcdbqi 356 13 semi semi JJ cord-104239-xxlcdbqi 356 14 - - JJ cord-104239-xxlcdbqi 356 15 intact intact JJ cord-104239-xxlcdbqi 356 16 cells cell NNS cord-104239-xxlcdbqi 356 17 were be VBD cord-104239-xxlcdbqi 356 18 fixed fix VBN cord-104239-xxlcdbqi 356 19 , , , cord-104239-xxlcdbqi 356 20 rapidly rapidly RB cord-104239-xxlcdbqi 356 21 frozen frozen JJ cord-104239-xxlcdbqi 356 22 , , , cord-104239-xxlcdbqi 356 23 and and CC cord-104239-xxlcdbqi 356 24 fractured fracture VBD cord-104239-xxlcdbqi 356 25 to to TO cord-104239-xxlcdbqi 356 26 expose expose VB cord-104239-xxlcdbqi 356 27 internal internal JJ cord-104239-xxlcdbqi 356 28 membranes membrane NNS cord-104239-xxlcdbqi 356 29 . . . cord-104239-xxlcdbqi 357 1 After after IN cord-104239-xxlcdbqi 357 2 etching etching NN cord-104239-xxlcdbqi 357 3 and and CC cord-104239-xxlcdbqi 357 4 replication replication NN cord-104239-xxlcdbqi 357 5 , , , cord-104239-xxlcdbqi 357 6 intracellular intracellular JJ cord-104239-xxlcdbqi 357 7 organelles organelle NNS cord-104239-xxlcdbqi 357 8 are be VBP cord-104239-xxlcdbqi 357 9 rendered render VBN cord-104239-xxlcdbqi 357 10 visible visible JJ cord-104239-xxlcdbqi 357 11 as as IN cord-104239-xxlcdbqi 357 12 three three CD cord-104239-xxlcdbqi 357 13 - - HYPH cord-104239-xxlcdbqi 357 14 dimensional dimensional JJ cord-104239-xxlcdbqi 357 15 structures structure NNS cord-104239-xxlcdbqi 357 16 . . . cord-104239-xxlcdbqi 358 1 The the DT cord-104239-xxlcdbqi 358 2 surface surface NN cord-104239-xxlcdbqi 358 3 of of IN cord-104239-xxlcdbqi 358 4 the the DT cord-104239-xxlcdbqi 358 5 ER ER NNP cord-104239-xxlcdbqi 358 6 was be VBD cord-104239-xxlcdbqi 358 7 readily readily RB cord-104239-xxlcdbqi 358 8 distinguishable distinguishable JJ cord-104239-xxlcdbqi 358 9 from from IN cord-104239-xxlcdbqi 358 10 other other JJ cord-104239-xxlcdbqi 358 11 subcellular subcellular JJ cord-104239-xxlcdbqi 358 12 compartments compartment NNS cord-104239-xxlcdbqi 358 13 by by IN cord-104239-xxlcdbqi 358 14 the the DT cord-104239-xxlcdbqi 358 15 presence presence NN cord-104239-xxlcdbqi 358 16 of of IN cord-104239-xxlcdbqi 358 17 ribosomes ribosome NNS cord-104239-xxlcdbqi 358 18 or or CC cord-104239-xxlcdbqi 358 19 , , , cord-104239-xxlcdbqi 358 20 in in IN cord-104239-xxlcdbqi 358 21 the the DT cord-104239-xxlcdbqi 358 22 case case NN cord-104239-xxlcdbqi 358 23 of of IN cord-104239-xxlcdbqi 358 24 the the DT cord-104239-xxlcdbqi 358 25 nuclear nuclear JJ cord-104239-xxlcdbqi 358 26 envelope envelope NN cord-104239-xxlcdbqi 358 27 , , , cord-104239-xxlcdbqi 358 28 additionally additionally RB cord-104239-xxlcdbqi 358 29 , , , cord-104239-xxlcdbqi 358 30 nuclear nuclear JJ cord-104239-xxlcdbqi 358 31 pores pore NNS cord-104239-xxlcdbqi 358 32 . . . cord-104239-xxlcdbqi 359 1 Adjacent adjacent JJ cord-104239-xxlcdbqi 359 2 to to IN cord-104239-xxlcdbqi 359 3 the the DT cord-104239-xxlcdbqi 359 4 surface surface NN cord-104239-xxlcdbqi 359 5 of of IN cord-104239-xxlcdbqi 359 6 the the DT cord-104239-xxlcdbqi 359 7 ER ER NNP cord-104239-xxlcdbqi 359 8 , , , cord-104239-xxlcdbqi 359 9 we -PRON- PRP cord-104239-xxlcdbqi 359 10 frequently frequently RB cord-104239-xxlcdbqi 359 11 observed observe VBD cord-104239-xxlcdbqi 359 12 compact compact JJ cord-104239-xxlcdbqi 359 13 structures structure NNS cord-104239-xxlcdbqi 359 14 of of IN cord-104239-xxlcdbqi 359 15 similar similar JJ cord-104239-xxlcdbqi 359 16 size size NN cord-104239-xxlcdbqi 359 17 and and CC cord-104239-xxlcdbqi 359 18 apparent apparent JJ cord-104239-xxlcdbqi 359 19 vesicular vesicular JJ cord-104239-xxlcdbqi 359 20 - - HYPH cord-104239-xxlcdbqi 359 21 tubular tubular JJ cord-104239-xxlcdbqi 359 22 composition composition NN cord-104239-xxlcdbqi 359 23 to to IN cord-104239-xxlcdbqi 359 24 VTCs vtc NNS cord-104239-xxlcdbqi 359 25 observed observe VBN cord-104239-xxlcdbqi 359 26 in in IN cord-104239-xxlcdbqi 359 27 thin thin JJ cord-104239-xxlcdbqi 359 28 - - HYPH cord-104239-xxlcdbqi 359 29 sections section NNS cord-104239-xxlcdbqi 359 30 ( ( -LRB- cord-104239-xxlcdbqi 359 31 Fig Fig NNP cord-104239-xxlcdbqi 359 32 . . . cord-104239-xxlcdbqi 359 33 9 9 CD cord-104239-xxlcdbqi 359 34 A a NN cord-104239-xxlcdbqi 359 35 ) ) -RRB- cord-104239-xxlcdbqi 359 36 . . . cord-104239-xxlcdbqi 360 1 These these DT cord-104239-xxlcdbqi 360 2 structures structure NNS cord-104239-xxlcdbqi 360 3 were be VBD cord-104239-xxlcdbqi 360 4 completely completely RB cord-104239-xxlcdbqi 360 5 absent absent JJ cord-104239-xxlcdbqi 360 6 in in IN cord-104239-xxlcdbqi 360 7 incubations incubation NNS cord-104239-xxlcdbqi 360 8 that that WDT cord-104239-xxlcdbqi 360 9 lacked lack VBD cord-104239-xxlcdbqi 360 10 cytosol cytosol NN cord-104239-xxlcdbqi 360 11 or or CC cord-104239-xxlcdbqi 360 12 ATP ATP NNP cord-104239-xxlcdbqi 360 13 . . . cord-104239-xxlcdbqi 361 1 They -PRON- PRP cord-104239-xxlcdbqi 361 2 varied vary VBD cord-104239-xxlcdbqi 361 3 in in IN cord-104239-xxlcdbqi 361 4 diameter diameter NN cord-104239-xxlcdbqi 361 5 , , , cord-104239-xxlcdbqi 361 6 but but CC cord-104239-xxlcdbqi 361 7 were be VBD cord-104239-xxlcdbqi 361 8 generally generally RB cord-104239-xxlcdbqi 361 9 ~0.3 ~0.3 CD cord-104239-xxlcdbqi 361 10 - - SYM cord-104239-xxlcdbqi 361 11 0.5 0.5 CD cord-104239-xxlcdbqi 362 1 ~m ~m JJ cord-104239-xxlcdbqi 362 2 across across RB cord-104239-xxlcdbqi 362 3 and and CC cord-104239-xxlcdbqi 362 4 ranged range VBD cord-104239-xxlcdbqi 362 5 from from IN cord-104239-xxlcdbqi 362 6 a a DT cord-104239-xxlcdbqi 362 7 circular circular NN cord-104239-xxlcdbqi 362 8 to to IN cord-104239-xxlcdbqi 362 9 a a DT cord-104239-xxlcdbqi 362 10 more more RBR cord-104239-xxlcdbqi 362 11 oblong oblong JJ cord-104239-xxlcdbqi 362 12 shape shape NN cord-104239-xxlcdbqi 362 13 under under IN cord-104239-xxlcdbqi 362 14 normal normal JJ cord-104239-xxlcdbqi 362 15 incubation incubation NN cord-104239-xxlcdbqi 362 16 conditions condition NNS cord-104239-xxlcdbqi 362 17 . . . cord-104239-xxlcdbqi 363 1 Assuming assume VBG cord-104239-xxlcdbqi 363 2 that that IN cord-104239-xxlcdbqi 363 3 the the DT cord-104239-xxlcdbqi 363 4 distinctive distinctive JJ cord-104239-xxlcdbqi 363 5 ~80-nm ~80-nm NNP cord-104239-xxlcdbqi 363 6 surface surface NN cord-104239-xxlcdbqi 363 7 undulations undulation NNS cord-104239-xxlcdbqi 363 8 correspond correspond VBP cord-104239-xxlcdbqi 363 9 to to IN cord-104239-xxlcdbqi 363 10 vesicle vesicle NN cord-104239-xxlcdbqi 363 11 profiles profile NNS cord-104239-xxlcdbqi 363 12 ( ( -LRB- cord-104239-xxlcdbqi 363 13 Fig Fig NNP cord-104239-xxlcdbqi 363 14 . . . cord-104239-xxlcdbqi 363 15 9 9 CD cord-104239-xxlcdbqi 363 16 A a CD cord-104239-xxlcdbqi 363 17 ) ) -RRB- cord-104239-xxlcdbqi 363 18 and and CC cord-104239-xxlcdbqi 363 19 that that IN cord-104239-xxlcdbqi 363 20 a a DT cord-104239-xxlcdbqi 363 21 cluster cluster NN cord-104239-xxlcdbqi 363 22 can can MD cord-104239-xxlcdbqi 363 23 be be VB cord-104239-xxlcdbqi 363 24 represented represent VBN cord-104239-xxlcdbqi 363 25 as as IN cord-104239-xxlcdbqi 363 26 a a DT cord-104239-xxlcdbqi 363 27 sphere sphere NN cord-104239-xxlcdbqi 363 28 with with IN cord-104239-xxlcdbqi 363 29 a a DT cord-104239-xxlcdbqi 363 30 similar similar JJ cord-104239-xxlcdbqi 363 31 range range NN cord-104239-xxlcdbqi 363 32 of of IN cord-104239-xxlcdbqi 363 33 diameters diameter NNS cord-104239-xxlcdbqi 363 34 , , , cord-104239-xxlcdbqi 363 35 we -PRON- PRP cord-104239-xxlcdbqi 363 36 estimate estimate VBP cord-104239-xxlcdbqi 363 37 that that IN cord-104239-xxlcdbqi 363 38 VTCs vtc NNS cord-104239-xxlcdbqi 363 39 detected detect VBD cord-104239-xxlcdbqi 363 40 in in IN cord-104239-xxlcdbqi 363 41 replicas replica NNS cord-104239-xxlcdbqi 363 42 could could MD cord-104239-xxlcdbqi 363 43 contain contain VB cord-104239-xxlcdbqi 363 44 50 50 CD cord-104239-xxlcdbqi 363 45 - - SYM cord-104239-xxlcdbqi 363 46 110 110 CD cord-104239-xxlcdbqi 363 47 individual individual JJ cord-104239-xxlcdbqi 363 48 elements element NNS cord-104239-xxlcdbqi 363 49 . . . cord-104239-xxlcdbqi 364 1 This this DT cord-104239-xxlcdbqi 364 2 value value NN cord-104239-xxlcdbqi 364 3 is be VBZ cord-104239-xxlcdbqi 364 4 compatible compatible JJ cord-104239-xxlcdbqi 364 5 with with IN cord-104239-xxlcdbqi 364 6 the the DT cord-104239-xxlcdbqi 364 7 number number NN cord-104239-xxlcdbqi 364 8 of of IN cord-104239-xxlcdbqi 364 9 vesicular vesicular JJ cord-104239-xxlcdbqi 364 10 profiles profile NNS cord-104239-xxlcdbqi 364 11 determined determine VBN cord-104239-xxlcdbqi 364 12 by by IN cord-104239-xxlcdbqi 364 13 reconstruction reconstruction NN cord-104239-xxlcdbqi 364 14 from from IN cord-104239-xxlcdbqi 364 15 serial serial JJ cord-104239-xxlcdbqi 364 16 thin thin JJ cord-104239-xxlcdbqi 364 17 - - HYPH cord-104239-xxlcdbqi 364 18 sections section NNS cord-104239-xxlcdbqi 364 19 . . . cord-104239-xxlcdbqi 365 1 To to TO cord-104239-xxlcdbqi 365 2 identify identify VB cord-104239-xxlcdbqi 365 3 whether whether IN cord-104239-xxlcdbqi 365 4 the the DT cord-104239-xxlcdbqi 365 5 above above JJ cord-104239-xxlcdbqi 365 6 structures structure NNS cord-104239-xxlcdbqi 365 7 formed form VBN cord-104239-xxlcdbqi 365 8 in in IN cord-104239-xxlcdbqi 365 9 the the DT cord-104239-xxlcdbqi 365 10 presence presence NN cord-104239-xxlcdbqi 365 11 of of IN cord-104239-xxlcdbqi 365 12 cytosol cytosol NN cord-104239-xxlcdbqi 365 13 and and CC cord-104239-xxlcdbqi 365 14 ATP ATP NNP cord-104239-xxlcdbqi 365 15 contained contain VBD cord-104239-xxlcdbqi 365 16 ER ER NNP cord-104239-xxlcdbqi 365 17 - - HYPH cord-104239-xxlcdbqi 365 18 derived derive VBN cord-104239-xxlcdbqi 365 19 cargo cargo NN cord-104239-xxlcdbqi 365 20 proteins protein NNS cord-104239-xxlcdbqi 365 21 such such JJ cord-104239-xxlcdbqi 365 22 as as IN cord-104239-xxlcdbqi 365 23 VSV VSV NNP cord-104239-xxlcdbqi 365 24 - - HYPH cord-104239-xxlcdbqi 365 25 G G NNP cord-104239-xxlcdbqi 365 26 , , , cord-104239-xxlcdbqi 365 27 NRK NRK NNP cord-104239-xxlcdbqi 365 28 cells cell NNS cord-104239-xxlcdbqi 365 29 were be VBD cord-104239-xxlcdbqi 365 30 infected infect VBN cord-104239-xxlcdbqi 365 31 with with IN cord-104239-xxlcdbqi 365 32 ts045 ts045 NNP cord-104239-xxlcdbqi 365 33 VSV VSV NNP cord-104239-xxlcdbqi 365 34 at at IN cord-104239-xxlcdbqi 365 35 the the DT cord-104239-xxlcdbqi 365 36 restrictive restrictive JJ cord-104239-xxlcdbqi 365 37 temperature temperature NN cord-104239-xxlcdbqi 365 38 . . . cord-104239-xxlcdbqi 366 1 After after IN cord-104239-xxlcdbqi 366 2 permeabilization permeabilization NN cord-104239-xxlcdbqi 366 3 and and CC cord-104239-xxlcdbqi 366 4 incubation incubation NN cord-104239-xxlcdbqi 366 5 in in IN cord-104239-xxlcdbqi 366 6 vitro vitro FW cord-104239-xxlcdbqi 366 7 at at IN cord-104239-xxlcdbqi 366 8 32 32 CD cord-104239-xxlcdbqi 366 9 ° ° NN cord-104239-xxlcdbqi 366 10 C c NN cord-104239-xxlcdbqi 366 11 , , , cord-104239-xxlcdbqi 366 12 cells cell NNS cord-104239-xxlcdbqi 366 13 were be VBD cord-104239-xxlcdbqi 366 14 immunolabeled immunolabele VBN cord-104239-xxlcdbqi 366 15 for for IN cord-104239-xxlcdbqi 366 16 VSV VSV NNP cord-104239-xxlcdbqi 366 17 - - HYPH cord-104239-xxlcdbqi 366 18 G G NNP cord-104239-xxlcdbqi 366 19 using use VBG cord-104239-xxlcdbqi 366 20 the the DT cord-104239-xxlcdbqi 366 21 immunodiffusion immunodiffusion NN cord-104239-xxlcdbqi 366 22 protocol protocol NN cord-104239-xxlcdbqi 366 23 and and CC cord-104239-xxlcdbqi 366 24 replicas replica NNS cord-104239-xxlcdbqi 366 25 were be VBD cord-104239-xxlcdbqi 366 26 prepared prepare VBN cord-104239-xxlcdbqi 366 27 . . . cord-104239-xxlcdbqi 367 1 While while IN cord-104239-xxlcdbqi 367 2 a a DT cord-104239-xxlcdbqi 367 3 combination combination NN cord-104239-xxlcdbqi 367 4 of of IN cord-104239-xxlcdbqi 367 5 prolonged prolong VBN cord-104239-xxlcdbqi 367 6 incubation incubation NN cord-104239-xxlcdbqi 367 7 using use VBG cord-104239-xxlcdbqi 367 8 mild mild JJ cord-104239-xxlcdbqi 367 9 fixation fixation NN cord-104239-xxlcdbqi 367 10 conditions condition NNS cord-104239-xxlcdbqi 367 11 to to TO cord-104239-xxlcdbqi 367 12 label label VB cord-104239-xxlcdbqi 367 13 VSV VSV NNP cord-104239-xxlcdbqi 367 14 - - HYPH cord-104239-xxlcdbqi 367 15 G G NNP cord-104239-xxlcdbqi 367 16 and and CC cord-104239-xxlcdbqi 367 17 the the DT cord-104239-xxlcdbqi 367 18 presence presence NN cord-104239-xxlcdbqi 367 19 of of IN cord-104239-xxlcdbqi 367 20 prominent prominent JJ cord-104239-xxlcdbqi 367 21 " " `` cord-104239-xxlcdbqi 367 22 gold gold JJ cord-104239-xxlcdbqi 367 23 shadows shadow NNS cord-104239-xxlcdbqi 367 24 " " '' cord-104239-xxlcdbqi 367 25 from from IN cord-104239-xxlcdbqi 367 26 the the DT cord-104239-xxlcdbqi 367 27 etching etching NN cord-104239-xxlcdbqi 367 28 and and CC cord-104239-xxlcdbqi 367 29 replication replication NN cord-104239-xxlcdbqi 367 30 reduces reduce VBZ cord-104239-xxlcdbqi 367 31 the the DT cord-104239-xxlcdbqi 367 32 ability ability NN cord-104239-xxlcdbqi 367 33 of of IN cord-104239-xxlcdbqi 367 34 the the DT cord-104239-xxlcdbqi 367 35 technique technique NN cord-104239-xxlcdbqi 367 36 to to TO cord-104239-xxlcdbqi 367 37 reveal reveal VB cord-104239-xxlcdbqi 367 38 surface surface NN cord-104239-xxlcdbqi 367 39 features feature NNS cord-104239-xxlcdbqi 367 40 of of IN cord-104239-xxlcdbqi 367 41 these these DT cord-104239-xxlcdbqi 367 42 immunogold immunogold NN cord-104239-xxlcdbqi 367 43 - - HYPH cord-104239-xxlcdbqi 367 44 labeled label VBN cord-104239-xxlcdbqi 367 45 clusters cluster NNS cord-104239-xxlcdbqi 367 46 , , , cord-104239-xxlcdbqi 367 47 the the DT cord-104239-xxlcdbqi 367 48 distribution distribution NN cord-104239-xxlcdbqi 367 49 of of IN cord-104239-xxlcdbqi 367 50 VSV VSV NNP cord-104239-xxlcdbqi 367 51 - - HYPH cord-104239-xxlcdbqi 367 52 G G NNP cord-104239-xxlcdbqi 367 53 reveals reveal VBZ cord-104239-xxlcdbqi 367 54 the the DT cord-104239-xxlcdbqi 367 55 striking striking JJ cord-104239-xxlcdbqi 367 56 role role NN cord-104239-xxlcdbqi 367 57 of of IN cord-104239-xxlcdbqi 367 58 COPII COPII NNP cord-104239-xxlcdbqi 367 59 in in IN cord-104239-xxlcdbqi 367 60 concentrative concentrative JJ cord-104239-xxlcdbqi 367 61 export export NN cord-104239-xxlcdbqi 367 62 . . . cord-104239-xxlcdbqi 368 1 Before before IN cord-104239-xxlcdbqi 368 2 incubation incubation NN cord-104239-xxlcdbqi 368 3 of of IN cord-104239-xxlcdbqi 368 4 semi semi JJ cord-104239-xxlcdbqi 368 5 - - JJ cord-104239-xxlcdbqi 368 6 intact intact JJ cord-104239-xxlcdbqi 368 7 cells cell NNS cord-104239-xxlcdbqi 368 8 at at IN cord-104239-xxlcdbqi 368 9 32 32 CD cord-104239-xxlcdbqi 368 10 ° ° NN cord-104239-xxlcdbqi 368 11 C c NN cord-104239-xxlcdbqi 368 12 , , , cord-104239-xxlcdbqi 368 13 gold gold NN cord-104239-xxlcdbqi 368 14 detected detect VBD cord-104239-xxlcdbqi 368 15 on on IN cord-104239-xxlcdbqi 368 16 the the DT cord-104239-xxlcdbqi 368 17 surface surface NN cord-104239-xxlcdbqi 368 18 of of IN cord-104239-xxlcdbqi 368 19 the the DT cord-104239-xxlcdbqi 368 20 ER ER NNP cord-104239-xxlcdbqi 368 21 ( ( -LRB- cord-104239-xxlcdbqi 368 22 Fig fig NN cord-104239-xxlcdbqi 368 23 . . . cord-104239-xxlcdbqi 369 1 10 10 CD cord-104239-xxlcdbqi 369 2 A a NN cord-104239-xxlcdbqi 369 3 , , , cord-104239-xxlcdbqi 369 4 arrowheads arrowhead NNS cord-104239-xxlcdbqi 369 5 ) ) -RRB- cord-104239-xxlcdbqi 369 6 and and CC cord-104239-xxlcdbqi 369 7 nuclear nuclear JJ cord-104239-xxlcdbqi 369 8 envelope envelope NN cord-104239-xxlcdbqi 369 9 ( ( -LRB- cord-104239-xxlcdbqi 369 10 Fig fig NN cord-104239-xxlcdbqi 369 11 . . . cord-104239-xxlcdbqi 370 1 10 10 CD cord-104239-xxlcdbqi 370 2 B b NN cord-104239-xxlcdbqi 370 3 , , , cord-104239-xxlcdbqi 370 4 arrowheads arrowhead NNS cord-104239-xxlcdbqi 370 5 ) ) -RRB- cord-104239-xxlcdbqi 370 6 was be VBD cord-104239-xxlcdbqi 370 7 distributed distribute VBN cord-104239-xxlcdbqi 370 8 in in IN cord-104239-xxlcdbqi 370 9 an an DT cord-104239-xxlcdbqi 370 10 apparent apparent JJ cord-104239-xxlcdbqi 370 11 random random JJ cord-104239-xxlcdbqi 370 12 manner manner NN cord-104239-xxlcdbqi 370 13 throughout throughout IN cord-104239-xxlcdbqi 370 14 the the DT cord-104239-xxlcdbqi 370 15 ER ER NNP cord-104239-xxlcdbqi 370 16 cisternal cisternal NNP cord-104239-xxlcdbqi 370 17 network network NN cord-104239-xxlcdbqi 371 1 ( ( -LRB- cord-104239-xxlcdbqi 371 2 Plutner Plutner NNP cord-104239-xxlcdbqi 371 3 et et NNP cord-104239-xxlcdbqi 371 4 al al NNP cord-104239-xxlcdbqi 371 5 . . NNP cord-104239-xxlcdbqi 371 6 , , , cord-104239-xxlcdbqi 371 7 1992 1992 CD cord-104239-xxlcdbqi 371 8 ; ; : cord-104239-xxlcdbqi 371 9 Balch Balch NNP cord-104239-xxlcdbqi 371 10 et et NNP cord-104239-xxlcdbqi 371 11 al al NNP cord-104239-xxlcdbqi 371 12 . . NNP cord-104239-xxlcdbqi 371 13 , , , cord-104239-xxlcdbqi 371 14 1994 1994 CD cord-104239-xxlcdbqi 371 15 ) ) -RRB- cord-104239-xxlcdbqi 371 16 . . . cord-104239-xxlcdbqi 372 1 The the DT cord-104239-xxlcdbqi 372 2 surface surface NN cord-104239-xxlcdbqi 372 3 density density NN cord-104239-xxlcdbqi 372 4 of of IN cord-104239-xxlcdbqi 372 5 VSV VSV NNP cord-104239-xxlcdbqi 372 6 - - HYPH cord-104239-xxlcdbqi 372 7 G G NNP cord-104239-xxlcdbqi 372 8 in in IN cord-104239-xxlcdbqi 372 9 these these DT cord-104239-xxlcdbqi 372 10 ER ER NNP cord-104239-xxlcdbqi 372 11 membranes membrane NNS cord-104239-xxlcdbqi 372 12 was be VBD cord-104239-xxlcdbqi 372 13 32 32 CD cord-104239-xxlcdbqi 372 14 + + CD cord-104239-xxlcdbqi 372 15 -6 -6 JJ cord-104239-xxlcdbqi 372 16 gold gold NN cord-104239-xxlcdbqi 372 17 particles particle NNS cord-104239-xxlcdbqi 372 18 per per IN cord-104239-xxlcdbqi 372 19 p p NN cord-104239-xxlcdbqi 372 20 ~ ~ CD cord-104239-xxlcdbqi 372 21 m m NN cord-104239-xxlcdbqi 372 22 2 2 CD cord-104239-xxlcdbqi 372 23 . . . cord-104239-xxlcdbqi 373 1 In in IN cord-104239-xxlcdbqi 373 2 contrast contrast NN cord-104239-xxlcdbqi 373 3 , , , cord-104239-xxlcdbqi 373 4 incubation incubation NN cord-104239-xxlcdbqi 373 5 for for IN cord-104239-xxlcdbqi 373 6 45 45 CD cord-104239-xxlcdbqi 373 7 min min NNS cord-104239-xxlcdbqi 373 8 in in IN cord-104239-xxlcdbqi 373 9 the the DT cord-104239-xxlcdbqi 373 10 presence presence NN cord-104239-xxlcdbqi 373 11 of of IN cord-104239-xxlcdbqi 373 12 cytosol cytosol NN cord-104239-xxlcdbqi 373 13 and and CC cord-104239-xxlcdbqi 373 14 ATP ATP NNP cord-104239-xxlcdbqi 373 15 led lead VBD cord-104239-xxlcdbqi 373 16 to to IN cord-104239-xxlcdbqi 373 17 a a DT cord-104239-xxlcdbqi 373 18 dramatic dramatic JJ cord-104239-xxlcdbqi 373 19 change change NN cord-104239-xxlcdbqi 373 20 in in IN cord-104239-xxlcdbqi 373 21 the the DT cord-104239-xxlcdbqi 373 22 distribution distribution NN cord-104239-xxlcdbqi 373 23 of of IN cord-104239-xxlcdbqi 373 24 VSV VSV NNP cord-104239-xxlcdbqi 373 25 - - HYPH cord-104239-xxlcdbqi 373 26 G G NNP cord-104239-xxlcdbqi 373 27 , , , cord-104239-xxlcdbqi 373 28 rearranging rearrange VBG cord-104239-xxlcdbqi 373 29 gold gold NN cord-104239-xxlcdbqi 373 30 particles particle NNS cord-104239-xxlcdbqi 373 31 to to IN cord-104239-xxlcdbqi 373 32 a a DT cord-104239-xxlcdbqi 373 33 limited limited JJ cord-104239-xxlcdbqi 373 34 number number NN cord-104239-xxlcdbqi 373 35 of of IN cord-104239-xxlcdbqi 373 36 VTCs vtc NNS cord-104239-xxlcdbqi 373 37 that that WDT cord-104239-xxlcdbqi 373 38 were be VBD cord-104239-xxlcdbqi 373 39 well well RB cord-104239-xxlcdbqi 373 40 isolated isolate VBN cord-104239-xxlcdbqi 373 41 from from IN cord-104239-xxlcdbqi 373 42 each each DT cord-104239-xxlcdbqi 373 43 other other JJ cord-104239-xxlcdbqi 374 1 ( ( -LRB- cord-104239-xxlcdbqi 374 2 Fig Fig NNP cord-104239-xxlcdbqi 374 3 . . . cord-104239-xxlcdbqi 374 4 10 10 CD cord-104239-xxlcdbqi 374 5 C c NN cord-104239-xxlcdbqi 374 6 ) ) -RRB- cord-104239-xxlcdbqi 374 7 . . . cord-104239-xxlcdbqi 375 1 The the DT cord-104239-xxlcdbqi 375 2 density density NN cord-104239-xxlcdbqi 375 3 of of IN cord-104239-xxlcdbqi 375 4 gold gold NN cord-104239-xxlcdbqi 375 5 over over IN cord-104239-xxlcdbqi 375 6 VTCs vtc NNS cord-104239-xxlcdbqi 375 7 projected project VBN cord-104239-xxlcdbqi 375 8 as as IN cord-104239-xxlcdbqi 375 9 a a DT cord-104239-xxlcdbqi 375 10 flat flat JJ cord-104239-xxlcdbqi 375 11 surface surface NN cord-104239-xxlcdbqi 375 12 parallel parallel JJ cord-104239-xxlcdbqi 375 13 to to IN cord-104239-xxlcdbqi 375 14 the the DT cord-104239-xxlcdbqi 375 15 ER ER NNP cord-104239-xxlcdbqi 375 16 membrane membrane NN cord-104239-xxlcdbqi 375 17 ( ( -LRB- cord-104239-xxlcdbqi 375 18 referred refer VBN cord-104239-xxlcdbqi 375 19 to to IN cord-104239-xxlcdbqi 375 20 as as IN cord-104239-xxlcdbqi 375 21 a a DT cord-104239-xxlcdbqi 375 22 planar planar JJ cord-104239-xxlcdbqi 375 23 projection projection NN cord-104239-xxlcdbqi 375 24 ) ) -RRB- cord-104239-xxlcdbqi 376 1 was be VBD cord-104239-xxlcdbqi 376 2 ~850 ~850 VBN cord-104239-xxlcdbqi 376 3 -+ -+ . cord-104239-xxlcdbqi 376 4 250 250 CD cord-104239-xxlcdbqi 376 5 gold gold NN cord-104239-xxlcdbqi 376 6 particles particle NNS cord-104239-xxlcdbqi 376 7 per per IN cord-104239-xxlcdbqi 376 8 p p NN cord-104239-xxlcdbqi 376 9 ~ ~ CD cord-104239-xxlcdbqi 376 10 m m NN cord-104239-xxlcdbqi 376 11 2 2 CD cord-104239-xxlcdbqi 376 12 , , , cord-104239-xxlcdbqi 376 13 a a DT cord-104239-xxlcdbqi 376 14 value value NN cord-104239-xxlcdbqi 376 15 markedly markedly RB cord-104239-xxlcdbqi 376 16 higher high JJR cord-104239-xxlcdbqi 376 17 than than IN cord-104239-xxlcdbqi 376 18 that that DT cord-104239-xxlcdbqi 376 19 observed observe VBD cord-104239-xxlcdbqi 376 20 in in IN cord-104239-xxlcdbqi 376 21 the the DT cord-104239-xxlcdbqi 376 22 plane plane NN cord-104239-xxlcdbqi 376 23 of of IN cord-104239-xxlcdbqi 376 24 the the DT cord-104239-xxlcdbqi 376 25 ER ER NNP cord-104239-xxlcdbqi 376 26 membrane membrane NN cord-104239-xxlcdbqi 376 27 before before IN cord-104239-xxlcdbqi 376 28 incubation incubation NN cord-104239-xxlcdbqi 376 29 . . . cord-104239-xxlcdbqi 377 1 We -PRON- PRP cord-104239-xxlcdbqi 377 2 next next RB cord-104239-xxlcdbqi 377 3 used use VBD cord-104239-xxlcdbqi 377 4 replicas replica NNS cord-104239-xxlcdbqi 377 5 to to TO cord-104239-xxlcdbqi 377 6 follow follow VB cord-104239-xxlcdbqi 377 7 the the DT cord-104239-xxlcdbqi 377 8 effects effect NNS cord-104239-xxlcdbqi 377 9 of of IN cord-104239-xxlcdbqi 377 10 GTP'yS gtp'ys CD cord-104239-xxlcdbqi 377 11 and and CC cord-104239-xxlcdbqi 377 12 the the DT cord-104239-xxlcdbqi 377 13 Sarl Sarl NNP cord-104239-xxlcdbqi 377 14 - - HYPH cord-104239-xxlcdbqi 377 15 GTP GTP NNP cord-104239-xxlcdbqi 377 16 restricted restrict VBN cord-104239-xxlcdbqi 377 17 mutant mutant NN cord-104239-xxlcdbqi 377 18 on on IN cord-104239-xxlcdbqi 377 19 budding bud VBG cord-104239-xxlcdbqi 377 20 and and CC cord-104239-xxlcdbqi 377 21 concentration concentration NN cord-104239-xxlcdbqi 377 22 of of IN cord-104239-xxlcdbqi 377 23 VSV VSV NNP cord-104239-xxlcdbqi 377 24 - - HYPH cord-104239-xxlcdbqi 377 25 G. G. NNP cord-104239-xxlcdbqi 378 1 As as IN cord-104239-xxlcdbqi 378 2 shown show VBN cord-104239-xxlcdbqi 378 3 in in IN cord-104239-xxlcdbqi 378 4 Fig Fig NNP cord-104239-xxlcdbqi 378 5 . . . cord-104239-xxlcdbqi 379 1 10 10 CD cord-104239-xxlcdbqi 380 1 ( ( -LRB- cord-104239-xxlcdbqi 380 2 B b NN cord-104239-xxlcdbqi 380 3 and and CC cord-104239-xxlcdbqi 380 4 C C NNP cord-104239-xxlcdbqi 380 5 ) ) -RRB- cord-104239-xxlcdbqi 380 6 , , , cord-104239-xxlcdbqi 380 7 incu- incu- NN cord-104239-xxlcdbqi 380 8 bation bation NN cord-104239-xxlcdbqi 380 9 of of IN cord-104239-xxlcdbqi 380 10 noninfected noninfected JJ cord-104239-xxlcdbqi 380 11 semi semi JJ cord-104239-xxlcdbqi 380 12 - - JJ cord-104239-xxlcdbqi 380 13 intact intact JJ cord-104239-xxlcdbqi 380 14 cells cell NNS cord-104239-xxlcdbqi 380 15 in in IN cord-104239-xxlcdbqi 380 16 the the DT cord-104239-xxlcdbqi 380 17 presence presence NN cord-104239-xxlcdbqi 380 18 of of IN cord-104239-xxlcdbqi 380 19 GTP~/S GTP~/S NNP cord-104239-xxlcdbqi 380 20 led lead VBD cord-104239-xxlcdbqi 380 21 to to IN cord-104239-xxlcdbqi 380 22 the the DT cord-104239-xxlcdbqi 380 23 accumulation accumulation NN cord-104239-xxlcdbqi 380 24 of of IN cord-104239-xxlcdbqi 380 25 vesicles vesicle NNS cord-104239-xxlcdbqi 380 26 ~8 ~8 CD cord-104239-xxlcdbqi 381 1 0 0 NFP cord-104239-xxlcdbqi 381 2 nm nm NN cord-104239-xxlcdbqi 381 3 in in IN cord-104239-xxlcdbqi 381 4 diameter diameter NN cord-104239-xxlcdbqi 381 5 that that DT cord-104239-xxlcdbqi 381 6 , , , cord-104239-xxlcdbqi 381 7 consistent consistent JJ cord-104239-xxlcdbqi 381 8 with with IN cord-104239-xxlcdbqi 381 9 T T NNP cord-104239-xxlcdbqi 381 10 E e NN cord-104239-xxlcdbqi 381 11 M m NN cord-104239-xxlcdbqi 381 12 ( ( -LRB- cord-104239-xxlcdbqi 381 13 Fig Fig NNP cord-104239-xxlcdbqi 381 14 . . NNP cord-104239-xxlcdbqi 381 15 7 7 CD cord-104239-xxlcdbqi 381 16 , , , cord-104239-xxlcdbqi 381 17 B b NN cord-104239-xxlcdbqi 381 18 and and CC cord-104239-xxlcdbqi 381 19 C C NNP cord-104239-xxlcdbqi 381 20 ) ) -RRB- cord-104239-xxlcdbqi 381 21 , , , cord-104239-xxlcdbqi 381 22 had have VBD cord-104239-xxlcdbqi 381 23 a a DT cord-104239-xxlcdbqi 381 24 zig zig NN cord-104239-xxlcdbqi 381 25 - - HYPH cord-104239-xxlcdbqi 381 26 zag zag NN cord-104239-xxlcdbqi 381 27 necklace necklace NN cord-104239-xxlcdbqi 381 28 - - HYPH cord-104239-xxlcdbqi 381 29 like like JJ cord-104239-xxlcdbqi 381 30 appearance appearance NN cord-104239-xxlcdbqi 381 31 when when WRB cord-104239-xxlcdbqi 381 32 collapsed collapse VBD cord-104239-xxlcdbqi 381 33 on on IN cord-104239-xxlcdbqi 381 34 the the DT cord-104239-xxlcdbqi 381 35 surface surface NN cord-104239-xxlcdbqi 381 36 of of IN cord-104239-xxlcdbqi 381 37 the the DT cord-104239-xxlcdbqi 381 38 ER ER NNP cord-104239-xxlcdbqi 381 39 . . . cord-104239-xxlcdbqi 382 1 Immunolabeling immunolabeling NN cord-104239-xxlcdbqi 382 2 of of IN cord-104239-xxlcdbqi 382 3 replicas replica NNS cord-104239-xxlcdbqi 382 4 formed form VBN cord-104239-xxlcdbqi 382 5 in in IN cord-104239-xxlcdbqi 382 6 the the DT cord-104239-xxlcdbqi 382 7 presence presence NN cord-104239-xxlcdbqi 382 8 of of IN cord-104239-xxlcdbqi 382 9 GTP~/S GTP~/S NNP cord-104239-xxlcdbqi 382 10 revealed reveal VBD cord-104239-xxlcdbqi 382 11 that that IN cord-104239-xxlcdbqi 382 12 VSV VSV NNP cord-104239-xxlcdbqi 382 13 - - HYPH cord-104239-xxlcdbqi 382 14 G G NNP cord-104239-xxlcdbqi 382 15 reached reach VBD cord-104239-xxlcdbqi 382 16 a a DT cord-104239-xxlcdbqi 382 17 density density NN cord-104239-xxlcdbqi 382 18 of of IN cord-104239-xxlcdbqi 382 19 800 800 CD cord-104239-xxlcdbqi 382 20 _ _ NN cord-104239-xxlcdbqi 382 21 200 200 CD cord-104239-xxlcdbqi 382 22 gold gold NN cord-104239-xxlcdbqi 382 23 particles particle NNS cord-104239-xxlcdbqi 382 24 per per IN cord-104239-xxlcdbqi 382 25 txm txm NNP cord-104239-xxlcdbqi 382 26 ~ ~ NFP cord-104239-xxlcdbqi 382 27 in in IN cord-104239-xxlcdbqi 382 28 planar planar JJ cord-104239-xxlcdbqi 382 29 projection projection NN cord-104239-xxlcdbqi 382 30 within within IN cord-104239-xxlcdbqi 382 31 clusters cluster NNS cord-104239-xxlcdbqi 382 32 . . . cord-104239-xxlcdbqi 383 1 When when WRB cord-104239-xxlcdbqi 383 2 incubations incubation NNS cord-104239-xxlcdbqi 383 3 were be VBD cord-104239-xxlcdbqi 383 4 carried carry VBN cord-104239-xxlcdbqi 383 5 out out RP cord-104239-xxlcdbqi 383 6 in in IN cord-104239-xxlcdbqi 383 7 the the DT cord-104239-xxlcdbqi 383 8 presence presence NN cord-104239-xxlcdbqi 383 9 of of IN cord-104239-xxlcdbqi 383 10 the the DT cord-104239-xxlcdbqi 383 11 Sarl Sarl NNP cord-104239-xxlcdbqi 383 12 - - HYPH cord-104239-xxlcdbqi 383 13 GTP GTP NNP cord-104239-xxlcdbqi 383 14 restricted restrict VBN cord-104239-xxlcdbqi 383 15 mutant mutant JJ cord-104239-xxlcdbqi 383 16 , , , cord-104239-xxlcdbqi 383 17 necklaces necklace NNS cord-104239-xxlcdbqi 383 18 ( ( -LRB- cord-104239-xxlcdbqi 383 19 not not RB cord-104239-xxlcdbqi 383 20 shown show VBN cord-104239-xxlcdbqi 383 21 ) ) -RRB- cord-104239-xxlcdbqi 383 22 and and CC cord-104239-xxlcdbqi 383 23 compact compact JJ cord-104239-xxlcdbqi 383 24 vesicular vesicular JJ cord-104239-xxlcdbqi 383 25 clusters cluster NNS cord-104239-xxlcdbqi 383 26 were be VBD cord-104239-xxlcdbqi 383 27 also also RB cord-104239-xxlcdbqi 383 28 observed observe VBN cord-104239-xxlcdbqi 383 29 on on IN cord-104239-xxlcdbqi 383 30 replicas replica NNS cord-104239-xxlcdbqi 383 31 ( ( -LRB- cord-104239-xxlcdbqi 383 32 Fig fig NN cord-104239-xxlcdbqi 383 33 . . . cord-104239-xxlcdbqi 383 34 9 9 CD cord-104239-xxlcdbqi 383 35 D d NN cord-104239-xxlcdbqi 383 36 ) ) -RRB- cord-104239-xxlcdbqi 383 37 . . . cord-104239-xxlcdbqi 384 1 In in IN cord-104239-xxlcdbqi 384 2 the the DT cord-104239-xxlcdbqi 384 3 presence presence NN cord-104239-xxlcdbqi 384 4 of of IN cord-104239-xxlcdbqi 384 5 the the DT cord-104239-xxlcdbqi 384 6 Sarl Sarl NNP cord-104239-xxlcdbqi 384 7 mutant mutant NN cord-104239-xxlcdbqi 384 8 , , , cord-104239-xxlcdbqi 384 9 VSV VSV NNP cord-104239-xxlcdbqi 384 10 - - HYPH cord-104239-xxlcdbqi 384 11 G G NNP cord-104239-xxlcdbqi 384 12 reached reach VBD cord-104239-xxlcdbqi 384 13 a a DT cord-104239-xxlcdbqi 384 14 density density NN cord-104239-xxlcdbqi 384 15 of of IN cord-104239-xxlcdbqi 384 16 700 700 CD cord-104239-xxlcdbqi 384 17 _ _ NN cord-104239-xxlcdbqi 384 18 + + SYM cord-104239-xxlcdbqi 384 19 200 200 CD cord-104239-xxlcdbqi 384 20 gold gold NN cord-104239-xxlcdbqi 384 21 particles particle NNS cord-104239-xxlcdbqi 384 22 per per IN cord-104239-xxlcdbqi 384 23 ~m ~m JJ cord-104239-xxlcdbqi 384 24 2 2 CD cord-104239-xxlcdbqi 384 25 in in IN cord-104239-xxlcdbqi 384 26 a a DT cord-104239-xxlcdbqi 384 27 planar planar JJ cord-104239-xxlcdbqi 384 28 projection projection NN cord-104239-xxlcdbqi 384 29 of of IN cord-104239-xxlcdbqi 384 30 the the DT cord-104239-xxlcdbqi 384 31 cluster cluster NN cord-104239-xxlcdbqi 384 32 ( ( -LRB- cord-104239-xxlcdbqi 384 33 Fig fig NN cord-104239-xxlcdbqi 384 34 . . . cord-104239-xxlcdbqi 385 1 10 10 CD cord-104239-xxlcdbqi 385 2 _SP cord-104239-xxlcdbqi 385 3 E E NNP cord-104239-xxlcdbqi 385 4 ) ) -RRB- cord-104239-xxlcdbqi 385 5 , , , cord-104239-xxlcdbqi 385 6 reinforcing reinforce VBG cord-104239-xxlcdbqi 385 7 our -PRON- PRP$ cord-104239-xxlcdbqi 385 8 previous previous JJ cord-104239-xxlcdbqi 385 9 observations observation NNS cord-104239-xxlcdbqi 385 10 that that IN cord-104239-xxlcdbqi 385 11 the the DT cord-104239-xxlcdbqi 385 12 budding bud VBG cord-104239-xxlcdbqi 385 13 activity activity NN cord-104239-xxlcdbqi 385 14 associated associate VBN cord-104239-xxlcdbqi 385 15 with with IN cord-104239-xxlcdbqi 385 16 export export NN cord-104239-xxlcdbqi 385 17 complexes complex NNS cord-104239-xxlcdbqi 385 18 involves involve VBZ cord-104239-xxlcdbqi 385 19 concentration concentration NN cord-104239-xxlcdbqi 385 20 . . . cord-104239-xxlcdbqi 386 1 We -PRON- PRP cord-104239-xxlcdbqi 386 2 have have VBP cord-104239-xxlcdbqi 386 3 provided provide VBN cord-104239-xxlcdbqi 386 4 the the DT cord-104239-xxlcdbqi 386 5 first first JJ cord-104239-xxlcdbqi 386 6 quantitative quantitative JJ cord-104239-xxlcdbqi 386 7 , , , cord-104239-xxlcdbqi 386 8 stereological stereological JJ cord-104239-xxlcdbqi 386 9 de de NN cord-104239-xxlcdbqi 386 10 - - HYPH cord-104239-xxlcdbqi 386 11 scription scription NN cord-104239-xxlcdbqi 386 12 of of IN cord-104239-xxlcdbqi 386 13 the the DT cord-104239-xxlcdbqi 386 14 three three CD cord-104239-xxlcdbqi 386 15 - - HYPH cord-104239-xxlcdbqi 386 16 dimensional dimensional JJ cord-104239-xxlcdbqi 386 17 organization organization NN cord-104239-xxlcdbqi 386 18 of of IN cord-104239-xxlcdbqi 386 19 cellular cellular JJ cord-104239-xxlcdbqi 386 20 structures structure NNS cord-104239-xxlcdbqi 386 21 involved involve VBN cord-104239-xxlcdbqi 386 22 in in IN cord-104239-xxlcdbqi 386 23 transport transport NN cord-104239-xxlcdbqi 386 24 of of IN cord-104239-xxlcdbqi 386 25 cargo cargo NN cord-104239-xxlcdbqi 386 26 from from IN cord-104239-xxlcdbqi 386 27 the the DT cord-104239-xxlcdbqi 386 28 E e NN cord-104239-xxlcdbqi 386 29 R r NN cord-104239-xxlcdbqi 386 30 to to IN cord-104239-xxlcdbqi 386 31 the the DT cord-104239-xxlcdbqi 386 32 Golgi Golgi NNP cord-104239-xxlcdbqi 386 33 apparatus apparatus NN cord-104239-xxlcdbqi 386 34 . . . cord-104239-xxlcdbqi 387 1 Export export NN cord-104239-xxlcdbqi 387 2 complexes complex NNS cord-104239-xxlcdbqi 387 3 have have VBP cord-104239-xxlcdbqi 387 4 a a DT cord-104239-xxlcdbqi 387 5 hierarchial hierarchial JJ cord-104239-xxlcdbqi 387 6 organization organization NN cord-104239-xxlcdbqi 387 7 that that WDT cord-104239-xxlcdbqi 387 8 can can MD cord-104239-xxlcdbqi 387 9 be be VB cord-104239-xxlcdbqi 387 10 conceptually conceptually RB cord-104239-xxlcdbqi 387 11 divided divide VBN cord-104239-xxlcdbqi 387 12 into into IN cord-104239-xxlcdbqi 387 13 three three CD cord-104239-xxlcdbqi 387 14 tiers tier NNS cord-104239-xxlcdbqi 387 15 ( ( -LRB- cord-104239-xxlcdbqi 387 16 Fig Fig NNP cord-104239-xxlcdbqi 387 17 . . . cord-104239-xxlcdbqi 387 18 11 11 CD cord-104239-xxlcdbqi 387 19 A A NNP cord-104239-xxlcdbqi 387 20 ) ) -RRB- cord-104239-xxlcdbqi 387 21 . . . cord-104239-xxlcdbqi 388 1 The the DT cord-104239-xxlcdbqi 388 2 first first JJ cord-104239-xxlcdbqi 388 3 tier tier NN cord-104239-xxlcdbqi 388 4 ( ( -LRB- cord-104239-xxlcdbqi 388 5 Fig fig NN cord-104239-xxlcdbqi 388 6 . . . cord-104239-xxlcdbqi 388 7 11 11 CD cord-104239-xxlcdbqi 388 8 A A NNP cord-104239-xxlcdbqi 388 9 , , , cord-104239-xxlcdbqi 388 10 dotted dotted JJ cord-104239-xxlcdbqi 388 11 box box NN cord-104239-xxlcdbqi 388 12 ) ) -RRB- cord-104239-xxlcdbqi 388 13 consists consist VBZ cord-104239-xxlcdbqi 388 14 of of IN cord-104239-xxlcdbqi 388 15 closely closely RB cord-104239-xxlcdbqi 388 16 adjacent adjacent JJ cord-104239-xxlcdbqi 388 17 buds bud NNS cord-104239-xxlcdbqi 388 18 on on IN cord-104239-xxlcdbqi 388 19 a a DT cord-104239-xxlcdbqi 388 20 single single JJ cord-104239-xxlcdbqi 388 21 E e NN cord-104239-xxlcdbqi 388 22 R r NN cord-104239-xxlcdbqi 388 23 cisterna cisterna NN cord-104239-xxlcdbqi 388 24 . . . cord-104239-xxlcdbqi 389 1 Each each DT cord-104239-xxlcdbqi 389 2 can can MD cord-104239-xxlcdbqi 389 3 give give VB cord-104239-xxlcdbqi 389 4 rise rise NN cord-104239-xxlcdbqi 389 5 to to IN cord-104239-xxlcdbqi 389 6 an an DT cord-104239-xxlcdbqi 389 7 individual individual JJ cord-104239-xxlcdbqi 389 8 string string NN cord-104239-xxlcdbqi 389 9 or or CC cord-104239-xxlcdbqi 389 10 group group NN cord-104239-xxlcdbqi 389 11 of of IN cord-104239-xxlcdbqi 389 12 ERderived erderived JJ cord-104239-xxlcdbqi 389 13 buds bud NNS cord-104239-xxlcdbqi 389 14 containing contain VBG cord-104239-xxlcdbqi 389 15 COPII COPII NNP cord-104239-xxlcdbqi 389 16 coats coat NNS cord-104239-xxlcdbqi 389 17 . . . cord-104239-xxlcdbqi 390 1 These these DT cord-104239-xxlcdbqi 390 2 budding bud VBG cord-104239-xxlcdbqi 390 3 loci locus NNS cord-104239-xxlcdbqi 390 4 were be VBD cord-104239-xxlcdbqi 390 5 limited limit VBN cord-104239-xxlcdbqi 390 6 to to IN cord-104239-xxlcdbqi 390 7 specific specific JJ cord-104239-xxlcdbqi 390 8 regions region NNS cord-104239-xxlcdbqi 390 9 of of IN cord-104239-xxlcdbqi 390 10 the the DT cord-104239-xxlcdbqi 390 11 E e NN cord-104239-xxlcdbqi 390 12 R r NN cord-104239-xxlcdbqi 390 13 in in IN cord-104239-xxlcdbqi 390 14 vivo vivo NN cord-104239-xxlcdbqi 390 15 , , , cord-104239-xxlcdbqi 390 16 suggesting suggest VBG cord-104239-xxlcdbqi 390 17 the the DT cord-104239-xxlcdbqi 390 18 existence existence NN cord-104239-xxlcdbqi 390 19 of of IN cord-104239-xxlcdbqi 390 20 a a DT cord-104239-xxlcdbqi 390 21 defined define VBN cord-104239-xxlcdbqi 390 22 number number NN cord-104239-xxlcdbqi 390 23 of of IN cord-104239-xxlcdbqi 390 24 export export NN cord-104239-xxlcdbqi 390 25 sites site NNS cord-104239-xxlcdbqi 390 26 in in IN cord-104239-xxlcdbqi 390 27 the the DT cord-104239-xxlcdbqi 390 28 living live VBG cord-104239-xxlcdbqi 390 29 cell cell NN cord-104239-xxlcdbqi 390 30 . . . cord-104239-xxlcdbqi 391 1 The the DT cord-104239-xxlcdbqi 391 2 second second JJ cord-104239-xxlcdbqi 391 3 tier tier NN cord-104239-xxlcdbqi 391 4 ( ( -LRB- cord-104239-xxlcdbqi 391 5 Fig fig NN cord-104239-xxlcdbqi 391 6 . . . cord-104239-xxlcdbqi 391 7 11 11 CD cord-104239-xxlcdbqi 391 8 A a DT cord-104239-xxlcdbqi 391 9 , , , cord-104239-xxlcdbqi 391 10 cylindrical cylindrical JJ cord-104239-xxlcdbqi 391 11 region region NN cord-104239-xxlcdbqi 391 12 outlined outline VBN cord-104239-xxlcdbqi 391 13 by by IN cord-104239-xxlcdbqi 391 14 dashed dash VBN cord-104239-xxlcdbqi 391 15 lines line NNS cord-104239-xxlcdbqi 391 16 ) ) -RRB- cord-104239-xxlcdbqi 391 17 comes come VBZ cord-104239-xxlcdbqi 391 18 from from IN cord-104239-xxlcdbqi 391 19 the the DT cord-104239-xxlcdbqi 391 20 observation observation NN cord-104239-xxlcdbqi 391 21 that that IN cord-104239-xxlcdbqi 391 22 buds bud NNS cord-104239-xxlcdbqi 391 23 on on IN cord-104239-xxlcdbqi 391 24 one one CD cord-104239-xxlcdbqi 391 25 cisterna cisterna NNP cord-104239-xxlcdbqi 391 26 were be VBD cord-104239-xxlcdbqi 391 27 often often RB cord-104239-xxlcdbqi 391 28 found find VBN cord-104239-xxlcdbqi 391 29 in in IN cord-104239-xxlcdbqi 391 30 close close JJ cord-104239-xxlcdbqi 391 31 proximity proximity NN cord-104239-xxlcdbqi 391 32 to to IN cord-104239-xxlcdbqi 391 33 budding bud VBG cord-104239-xxlcdbqi 391 34 profiles profile NNS cord-104239-xxlcdbqi 391 35 emanating emanate VBG cord-104239-xxlcdbqi 391 36 from from IN cord-104239-xxlcdbqi 391 37 E E NNP cord-104239-xxlcdbqi 391 38 R r NN cord-104239-xxlcdbqi 391 39 cisternae cisternae NN cord-104239-xxlcdbqi 391 40 derived derive VBN cord-104239-xxlcdbqi 391 41 from from IN cord-104239-xxlcdbqi 391 42 distantly distantly RB cord-104239-xxlcdbqi 391 43 connected connect VBN cord-104239-xxlcdbqi 391 44 regions region NNS cord-104239-xxlcdbqi 391 45 of of IN cord-104239-xxlcdbqi 391 46 the the DT cord-104239-xxlcdbqi 391 47 ER ER NNP cord-104239-xxlcdbqi 391 48 . . . cord-104239-xxlcdbqi 392 1 The the DT cord-104239-xxlcdbqi 392 2 third third JJ cord-104239-xxlcdbqi 392 3 tier tier NN cord-104239-xxlcdbqi 392 4 of of IN cord-104239-xxlcdbqi 392 5 organization organization NN cord-104239-xxlcdbqi 392 6 encompassing encompass VBG cord-104239-xxlcdbqi 392 7 the the DT cord-104239-xxlcdbqi 392 8 entire entire JJ cord-104239-xxlcdbqi 392 9 export export NN cord-104239-xxlcdbqi 392 10 complex complex NN cord-104239-xxlcdbqi 392 11 ( ( -LRB- cord-104239-xxlcdbqi 392 12 Fig Fig NNP cord-104239-xxlcdbqi 392 13 . . . cord-104239-xxlcdbqi 392 14 11 11 CD cord-104239-xxlcdbqi 392 15 A a DT cord-104239-xxlcdbqi 392 16 , , , cord-104239-xxlcdbqi 392 17 solid solid JJ cord-104239-xxlcdbqi 392 18 box box NN cord-104239-xxlcdbqi 392 19 ) ) -RRB- cord-104239-xxlcdbqi 392 20 includes include VBZ cord-104239-xxlcdbqi 392 21 ER ER NNP cord-104239-xxlcdbqi 392 22 - - HYPH cord-104239-xxlcdbqi 392 23 derived derive VBN cord-104239-xxlcdbqi 392 24 buds bud NNS cord-104239-xxlcdbqi 392 25 that that WDT cord-104239-xxlcdbqi 392 26 face face VBP cord-104239-xxlcdbqi 392 27 into into IN cord-104239-xxlcdbqi 392 28 a a DT cord-104239-xxlcdbqi 392 29 region region NN cord-104239-xxlcdbqi 392 30 housing housing NN cord-104239-xxlcdbqi 392 31 a a DT cord-104239-xxlcdbqi 392 32 central central JJ cord-104239-xxlcdbqi 392 33 VTC vtc NN cord-104239-xxlcdbqi 392 34 . . . cord-104239-xxlcdbqi 393 1 Tubular tubular JJ cord-104239-xxlcdbqi 393 2 elements element NNS cord-104239-xxlcdbqi 393 3 within within IN cord-104239-xxlcdbqi 393 4 VTCs vtc NNS cord-104239-xxlcdbqi 393 5 contain contain VBP cord-104239-xxlcdbqi 393 6 distinctive distinctive JJ cord-104239-xxlcdbqi 393 7 COPI COPI NNP cord-104239-xxlcdbqi 393 8 coats coat NNS cord-104239-xxlcdbqi 393 9 and and CC cord-104239-xxlcdbqi 393 10 are be VBP cord-104239-xxlcdbqi 393 11 luminally luminally RB cord-104239-xxlcdbqi 393 12 discontinuous discontinuous JJ cord-104239-xxlcdbqi 393 13 with with IN cord-104239-xxlcdbqi 393 14 the the DT cord-104239-xxlcdbqi 393 15 ER ER NNP cord-104239-xxlcdbqi 393 16 . . . cord-104239-xxlcdbqi 394 1 While while IN cord-104239-xxlcdbqi 394 2 professional professional JJ cord-104239-xxlcdbqi 394 3 secretory secretory NN cord-104239-xxlcdbqi 394 4 Figure figure NN cord-104239-xxlcdbqi 394 5 10 10 CD cord-104239-xxlcdbqi 394 6 . . . cord-104239-xxlcdbqi 395 1 VSV VSV NNP cord-104239-xxlcdbqi 395 2 - - HYPH cord-104239-xxlcdbqi 395 3 G G NNP cord-104239-xxlcdbqi 395 4 is be VBZ cord-104239-xxlcdbqi 395 5 concentrated concentrate VBN cord-104239-xxlcdbqi 395 6 in in IN cord-104239-xxlcdbqi 395 7 ER er NN cord-104239-xxlcdbqi 395 8 - - HYPH cord-104239-xxlcdbqi 395 9 derived derive VBN cord-104239-xxlcdbqi 395 10 vesicles vesicle NNS cord-104239-xxlcdbqi 395 11 and and CC cord-104239-xxlcdbqi 395 12 VTCs vtc NNS cord-104239-xxlcdbqi 395 13 . . . cord-104239-xxlcdbqi 396 1 NRK NRK NNP cord-104239-xxlcdbqi 396 2 cells cell NNS cord-104239-xxlcdbqi 396 3 infected infect VBN cord-104239-xxlcdbqi 396 4 with with IN cord-104239-xxlcdbqi 396 5 ts045 ts045 NNP cord-104239-xxlcdbqi 396 6 VSV VSV NNP cord-104239-xxlcdbqi 396 7 at at IN cord-104239-xxlcdbqi 396 8 the the DT cord-104239-xxlcdbqi 396 9 restrictive restrictive JJ cord-104239-xxlcdbqi 396 10 temperature temperature NN cord-104239-xxlcdbqi 396 11 ( ( -LRB- cord-104239-xxlcdbqi 396 12 39.5 39.5 CD cord-104239-xxlcdbqi 396 13 ° ° NN cord-104239-xxlcdbqi 396 14 C c NN cord-104239-xxlcdbqi 396 15 ) ) -RRB- cord-104239-xxlcdbqi 396 16 to to TO cord-104239-xxlcdbqi 396 17 retain retain VB cord-104239-xxlcdbqi 396 18 VSV VSV NNP cord-104239-xxlcdbqi 396 19 - - HYPH cord-104239-xxlcdbqi 396 20 G G NNP cord-104239-xxlcdbqi 396 21 in in IN cord-104239-xxlcdbqi 396 22 the the DT cord-104239-xxlcdbqi 396 23 ER ER NNP cord-104239-xxlcdbqi 396 24 were be VBD cord-104239-xxlcdbqi 396 25 permeabilized permeabilize VBN cord-104239-xxlcdbqi 396 26 with with IN cord-104239-xxlcdbqi 396 27 digitonin digitonin NNP cord-104239-xxlcdbqi 397 1 ( ( -LRB- cord-104239-xxlcdbqi 397 2 Plutner Plutner NNP cord-104239-xxlcdbqi 397 3 et et NNP cord-104239-xxlcdbqi 397 4 al al NNP cord-104239-xxlcdbqi 397 5 . . NNP cord-104239-xxlcdbqi 397 6 , , , cord-104239-xxlcdbqi 397 7 1992 1992 CD cord-104239-xxlcdbqi 397 8 ) ) -RRB- cord-104239-xxlcdbqi 397 9 and and CC cord-104239-xxlcdbqi 397 10 either either CC cord-104239-xxlcdbqi 397 11 fixed fix VBN cord-104239-xxlcdbqi 397 12 immediately immediately RB cord-104239-xxlcdbqi 398 1 ( ( -LRB- cord-104239-xxlcdbqi 398 2 A a NN cord-104239-xxlcdbqi 398 3 and and CC cord-104239-xxlcdbqi 398 4 B b NN cord-104239-xxlcdbqi 398 5 ) ) -RRB- cord-104239-xxlcdbqi 398 6 or or CC cord-104239-xxlcdbqi 398 7 incubated incubate VBN cord-104239-xxlcdbqi 398 8 for for IN cord-104239-xxlcdbqi 398 9 45 45 CD cord-104239-xxlcdbqi 398 10 rain rain NN cord-104239-xxlcdbqi 398 11 at at IN cord-104239-xxlcdbqi 398 12 the the DT cord-104239-xxlcdbqi 398 13 permissive permissive JJ cord-104239-xxlcdbqi 398 14 temperature temperature NN cord-104239-xxlcdbqi 398 15 ( ( -LRB- cord-104239-xxlcdbqi 398 16 32 32 CD cord-104239-xxlcdbqi 398 17 ° ° NN cord-104239-xxlcdbqi 398 18 C C NNP cord-104239-xxlcdbqi 398 19 ) ) -RRB- cord-104239-xxlcdbqi 398 20 in in IN cord-104239-xxlcdbqi 398 21 the the DT cord-104239-xxlcdbqi 398 22 presence presence NN cord-104239-xxlcdbqi 398 23 of of IN cord-104239-xxlcdbqi 398 24 cytosol cytosol NN cord-104239-xxlcdbqi 398 25 and and CC cord-104239-xxlcdbqi 398 26 ATP ATP NNP cord-104239-xxlcdbqi 398 27 ( ( -LRB- cord-104239-xxlcdbqi 398 28 C C NNP cord-104239-xxlcdbqi 398 29 ) ) -RRB- cord-104239-xxlcdbqi 398 30 , , , cord-104239-xxlcdbqi 398 31 or or CC cord-104239-xxlcdbqi 398 32 additionally additionally RB cord-104239-xxlcdbqi 398 33 supplemented supplement VBN cord-104239-xxlcdbqi 398 34 with with IN cord-104239-xxlcdbqi 398 35 1 1 CD cord-104239-xxlcdbqi 398 36 IxM ixm NN cord-104239-xxlcdbqi 398 37 Sarl[H79 sarl[h79 NN cord-104239-xxlcdbqi 398 38 G G NNP cord-104239-xxlcdbqi 398 39 ] ] -RRB- cord-104239-xxlcdbqi 398 40 ( ( -LRB- cord-104239-xxlcdbqi 398 41 E e NN cord-104239-xxlcdbqi 398 42 ) ) -RRB- cord-104239-xxlcdbqi 398 43 . . . cord-104239-xxlcdbqi 399 1 After after IN cord-104239-xxlcdbqi 399 2 incubation incubation NN cord-104239-xxlcdbqi 399 3 , , , cord-104239-xxlcdbqi 399 4 cells cell NNS cord-104239-xxlcdbqi 399 5 were be VBD cord-104239-xxlcdbqi 399 6 fixed fix VBN cord-104239-xxlcdbqi 399 7 and and CC cord-104239-xxlcdbqi 399 8 VSV VSV NNP cord-104239-xxlcdbqi 399 9 - - HYPH cord-104239-xxlcdbqi 399 10 G G NNP cord-104239-xxlcdbqi 399 11 labeled label VBN cord-104239-xxlcdbqi 399 12 with with IN cord-104239-xxlcdbqi 399 13 10 10 CD cord-104239-xxlcdbqi 399 14 nm nm NN cord-104239-xxlcdbqi 399 15 ( ( -LRB- cord-104239-xxlcdbqi 399 16 A A NNP cord-104239-xxlcdbqi 399 17 - - HYPH cord-104239-xxlcdbqi 399 18 C C NNP cord-104239-xxlcdbqi 399 19 , , , cord-104239-xxlcdbqi 399 20 and and CC cord-104239-xxlcdbqi 399 21 E E NNP cord-104239-xxlcdbqi 399 22 ) ) -RRB- cord-104239-xxlcdbqi 399 23 or or CC cord-104239-xxlcdbqi 399 24 6 6 CD cord-104239-xxlcdbqi 399 25 nm nm NN cord-104239-xxlcdbqi 399 26 ( ( -LRB- cord-104239-xxlcdbqi 399 27 D d NN cord-104239-xxlcdbqi 399 28 ) ) -RRB- cord-104239-xxlcdbqi 399 29 gold gold NN cord-104239-xxlcdbqi 399 30 particles particle NNS cord-104239-xxlcdbqi 399 31 using use VBG cord-104239-xxlcdbqi 399 32 the the DT cord-104239-xxlcdbqi 399 33 immunodiffusion immunodiffusion NN cord-104239-xxlcdbqi 399 34 protocol protocol NN cord-104239-xxlcdbqi 399 35 as as IN cord-104239-xxlcdbqi 399 36 described describe VBN cord-104239-xxlcdbqi 399 37 in in IN cord-104239-xxlcdbqi 399 38 the the DT cord-104239-xxlcdbqi 399 39 Materials Materials NNPS cord-104239-xxlcdbqi 399 40 and and CC cord-104239-xxlcdbqi 399 41 Methods method NNS cord-104239-xxlcdbqi 399 42 . . . cord-104239-xxlcdbqi 400 1 Cells cell NNS cord-104239-xxlcdbqi 400 2 were be VBD cord-104239-xxlcdbqi 400 3 either either RB cord-104239-xxlcdbqi 400 4 prepared prepare VBN cord-104239-xxlcdbqi 400 5 for for IN cord-104239-xxlcdbqi 400 6 thin thin JJ cord-104239-xxlcdbqi 400 7 - - HYPH cord-104239-xxlcdbqi 400 8 section section NN cord-104239-xxlcdbqi 400 9 TEM tem NN cord-104239-xxlcdbqi 400 10 ( ( -LRB- cord-104239-xxlcdbqi 400 11 D D NNP cord-104239-xxlcdbqi 400 12 ) ) -RRB- cord-104239-xxlcdbqi 400 13 or or CC cord-104239-xxlcdbqi 400 14 for for IN cord-104239-xxlcdbqi 400 15 quickfreeze quickfreeze NNP cord-104239-xxlcdbqi 400 16 , , , cord-104239-xxlcdbqi 400 17 deep deep JJ cord-104239-xxlcdbqi 400 18 - - HYPH cord-104239-xxlcdbqi 400 19 etch etch NN cord-104239-xxlcdbqi 400 20 replication replication NN cord-104239-xxlcdbqi 400 21 ( ( -LRB- cord-104239-xxlcdbqi 400 22 A A NNP cord-104239-xxlcdbqi 400 23 - - HYPH cord-104239-xxlcdbqi 400 24 C C NNP cord-104239-xxlcdbqi 400 25 , , , cord-104239-xxlcdbqi 400 26 and and CC cord-104239-xxlcdbqi 400 27 E e LS cord-104239-xxlcdbqi 400 28 ) ) -RRB- cord-104239-xxlcdbqi 400 29 . . . cord-104239-xxlcdbqi 401 1 At at IN cord-104239-xxlcdbqi 401 2 the the DT cord-104239-xxlcdbqi 401 3 restrictive restrictive JJ cord-104239-xxlcdbqi 401 4 temperature temperature NN cord-104239-xxlcdbqi 401 5 , , , cord-104239-xxlcdbqi 401 6 VSV VSV NNP cord-104239-xxlcdbqi 401 7 - - HYPH cord-104239-xxlcdbqi 401 8 G G NNP cord-104239-xxlcdbqi 401 9 was be VBD cord-104239-xxlcdbqi 401 10 uniformly uniformly RB cord-104239-xxlcdbqi 401 11 distributed distribute VBN cord-104239-xxlcdbqi 401 12 within within IN cord-104239-xxlcdbqi 401 13 the the DT cord-104239-xxlcdbqi 401 14 ER ER NNP cord-104239-xxlcdbqi 401 15 membrane membrane NN cord-104239-xxlcdbqi 401 16 ( ( -LRB- cord-104239-xxlcdbqi 401 17 A a NN cord-104239-xxlcdbqi 401 18 ) ) -RRB- cord-104239-xxlcdbqi 401 19 or or CC cord-104239-xxlcdbqi 401 20 the the DT cord-104239-xxlcdbqi 401 21 nuclear nuclear JJ cord-104239-xxlcdbqi 401 22 envelope envelope NN cord-104239-xxlcdbqi 401 23 ( ( -LRB- cord-104239-xxlcdbqi 401 24 B b NN cord-104239-xxlcdbqi 401 25 ) ) -RRB- cord-104239-xxlcdbqi 401 26 . . . cord-104239-xxlcdbqi 402 1 After after IN cord-104239-xxlcdbqi 402 2 a a DT cord-104239-xxlcdbqi 402 3 shift shift NN cord-104239-xxlcdbqi 402 4 to to IN cord-104239-xxlcdbqi 402 5 the the DT cord-104239-xxlcdbqi 402 6 permissive permissive JJ cord-104239-xxlcdbqi 402 7 temperature temperature NN cord-104239-xxlcdbqi 402 8 ( ( -LRB- cord-104239-xxlcdbqi 402 9 B b NN cord-104239-xxlcdbqi 402 10 - - HYPH cord-104239-xxlcdbqi 402 11 E E NNP cord-104239-xxlcdbqi 402 12 ) ) -RRB- cord-104239-xxlcdbqi 402 13 , , , cord-104239-xxlcdbqi 402 14 VSV VSV NNP cord-104239-xxlcdbqi 402 15 - - HYPH cord-104239-xxlcdbqi 402 16 G G NNP cord-104239-xxlcdbqi 402 17 was be VBD cord-104239-xxlcdbqi 402 18 concentrated concentrate VBN cord-104239-xxlcdbqi 402 19 in in IN cord-104239-xxlcdbqi 402 20 newly newly RB cord-104239-xxlcdbqi 402 21 formed form VBN cord-104239-xxlcdbqi 402 22 80-nm 80-nm CD cord-104239-xxlcdbqi 402 23 vesicles vesicle NNS cord-104239-xxlcdbqi 402 24 associated associate VBN cord-104239-xxlcdbqi 402 25 with with IN cord-104239-xxlcdbqi 402 26 export export NN cord-104239-xxlcdbqi 402 27 complexes complex NNS cord-104239-xxlcdbqi 402 28 . . . cord-104239-xxlcdbqi 403 1 ( ( -LRB- cord-104239-xxlcdbqi 403 2 Arrowheads arrowhead NNS cord-104239-xxlcdbqi 403 3 ) ) -RRB- cord-104239-xxlcdbqi 403 4 Location location NN cord-104239-xxlcdbqi 403 5 of of IN cord-104239-xxlcdbqi 403 6 6 6 CD cord-104239-xxlcdbqi 403 7 nm nm NN cord-104239-xxlcdbqi 403 8 ( ( -LRB- cord-104239-xxlcdbqi 403 9 D D NNP cord-104239-xxlcdbqi 403 10 ) ) -RRB- cord-104239-xxlcdbqi 403 11 or or CC cord-104239-xxlcdbqi 403 12 10 10 CD cord-104239-xxlcdbqi 403 13 nm nm NN cord-104239-xxlcdbqi 403 14 ( ( -LRB- cord-104239-xxlcdbqi 403 15 A A NNP cord-104239-xxlcdbqi 403 16 - - HYPH cord-104239-xxlcdbqi 403 17 C C NNP cord-104239-xxlcdbqi 403 18 , , , cord-104239-xxlcdbqi 403 19 and and CC cord-104239-xxlcdbqi 403 20 E e NN cord-104239-xxlcdbqi 403 21 ) ) -RRB- cord-104239-xxlcdbqi 403 22 gold gold NN cord-104239-xxlcdbqi 403 23 particles particle NNS cord-104239-xxlcdbqi 403 24 corresponding correspond VBG cord-104239-xxlcdbqi 403 25 to to IN cord-104239-xxlcdbqi 403 26 the the DT cord-104239-xxlcdbqi 403 27 distribution distribution NN cord-104239-xxlcdbqi 403 28 of of IN cord-104239-xxlcdbqi 403 29 VSV VSV NNP cord-104239-xxlcdbqi 403 30 - - HYPH cord-104239-xxlcdbqi 403 31 G. G. NNP cord-104239-xxlcdbqi 404 1 Due due IN cord-104239-xxlcdbqi 404 2 to to IN cord-104239-xxlcdbqi 404 3 the the DT cord-104239-xxlcdbqi 404 4 use use NN cord-104239-xxlcdbqi 404 5 of of IN cord-104239-xxlcdbqi 404 6 an an DT cord-104239-xxlcdbqi 404 7 immunodiffusion immunodiffusion NN cord-104239-xxlcdbqi 404 8 protocol protocol NN cord-104239-xxlcdbqi 404 9 before before IN cord-104239-xxlcdbqi 404 10 preparation preparation NN cord-104239-xxlcdbqi 404 11 of of IN cord-104239-xxlcdbqi 404 12 replicas replica NNS cord-104239-xxlcdbqi 404 13 and and CC cord-104239-xxlcdbqi 404 14 the the DT cord-104239-xxlcdbqi 404 15 high high JJ cord-104239-xxlcdbqi 404 16 density density NN cord-104239-xxlcdbqi 404 17 of of IN cord-104239-xxlcdbqi 404 18 label label NN cord-104239-xxlcdbqi 404 19 , , , cord-104239-xxlcdbqi 404 20 extended extended JJ cord-104239-xxlcdbqi 404 21 shadows shadow NNS cord-104239-xxlcdbqi 404 22 from from IN cord-104239-xxlcdbqi 404 23 the the DT cord-104239-xxlcdbqi 404 24 gold gold NN cord-104239-xxlcdbqi 404 25 particles particle NNS cord-104239-xxlcdbqi 404 26 partially partially RB cord-104239-xxlcdbqi 404 27 obscure obscure JJ cord-104239-xxlcdbqi 404 28 membrane membrane NN cord-104239-xxlcdbqi 404 29 outlines outline NNS cord-104239-xxlcdbqi 404 30 . . . cord-104239-xxlcdbqi 405 1 Bar bar NN cord-104239-xxlcdbqi 405 2 , , , cord-104239-xxlcdbqi 405 3 0.1 0.1 CD cord-104239-xxlcdbqi 405 4 I i NN cord-104239-xxlcdbqi 405 5 ~ ~ CD cord-104239-xxlcdbqi 405 6 m m NN cord-104239-xxlcdbqi 405 7 . . . cord-104239-xxlcdbqi 405 8 cells cell NNS cord-104239-xxlcdbqi 405 9 such such JJ cord-104239-xxlcdbqi 405 10 as as IN cord-104239-xxlcdbqi 405 11 those those DT cord-104239-xxlcdbqi 405 12 found find VBN cord-104239-xxlcdbqi 405 13 in in IN cord-104239-xxlcdbqi 405 14 the the DT cord-104239-xxlcdbqi 405 15 pancreas pancrea NNS cord-104239-xxlcdbqi 405 16 confine confine VBP cord-104239-xxlcdbqi 405 17 export export NN cord-104239-xxlcdbqi 405 18 predominately predominately RB cord-104239-xxlcdbqi 405 19 to to IN cord-104239-xxlcdbqi 405 20 a a DT cord-104239-xxlcdbqi 405 21 single single JJ cord-104239-xxlcdbqi 405 22 transitional transitional JJ cord-104239-xxlcdbqi 405 23 region region NN cord-104239-xxlcdbqi 405 24 juxtaposed juxtapose VBN cord-104239-xxlcdbqi 405 25 to to IN cord-104239-xxlcdbqi 405 26 the the DT cord-104239-xxlcdbqi 405 27 cis cis NNP cord-104239-xxlcdbqi 405 28 face face NN cord-104239-xxlcdbqi 405 29 of of IN cord-104239-xxlcdbqi 405 30 the the DT cord-104239-xxlcdbqi 405 31 Golgi Golgi NNP cord-104239-xxlcdbqi 405 32 apparatus apparatus NN cord-104239-xxlcdbqi 405 33 ( ( -LRB- cord-104239-xxlcdbqi 405 34 Palade Palade NNP cord-104239-xxlcdbqi 405 35 , , , cord-104239-xxlcdbqi 405 36 1975 1975 CD cord-104239-xxlcdbqi 405 37 ) ) -RRB- cord-104239-xxlcdbqi 405 38 , , , cord-104239-xxlcdbqi 405 39 the the DT cord-104239-xxlcdbqi 405 40 two two CD cord-104239-xxlcdbqi 405 41 different different JJ cord-104239-xxlcdbqi 405 42 cell cell NN cord-104239-xxlcdbqi 405 43 lines line NNS cord-104239-xxlcdbqi 405 44 used use VBN cord-104239-xxlcdbqi 405 45 in in IN cord-104239-xxlcdbqi 405 46 the the DT cord-104239-xxlcdbqi 405 47 present present JJ cord-104239-xxlcdbqi 405 48 study study NN cord-104239-xxlcdbqi 405 49 were be VBD cord-104239-xxlcdbqi 405 50 found find VBN cord-104239-xxlcdbqi 405 51 to to TO cord-104239-xxlcdbqi 405 52 have have VB cord-104239-xxlcdbqi 405 53 export export NN cord-104239-xxlcdbqi 405 54 complexes complex NNS cord-104239-xxlcdbqi 405 55 distributed distribute VBN cord-104239-xxlcdbqi 405 56 throughout throughout IN cord-104239-xxlcdbqi 405 57 the the DT cord-104239-xxlcdbqi 405 58 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 405 59 . . . cord-104239-xxlcdbqi 406 1 Our -PRON- PRP$ cord-104239-xxlcdbqi 406 2 studies study NNS cord-104239-xxlcdbqi 406 3 provide provide VBP cord-104239-xxlcdbqi 406 4 evidence evidence NN cord-104239-xxlcdbqi 406 5 that that IN cord-104239-xxlcdbqi 406 6 budding bud VBG cord-104239-xxlcdbqi 406 7 from from IN cord-104239-xxlcdbqi 406 8 the the DT cord-104239-xxlcdbqi 406 9 E e NN cord-104239-xxlcdbqi 406 10 R r NN cord-104239-xxlcdbqi 406 11 occurs occur VBZ cord-104239-xxlcdbqi 406 12 in in IN cord-104239-xxlcdbqi 406 13 areas area NNS cord-104239-xxlcdbqi 406 14 of of IN cord-104239-xxlcdbqi 406 15 intense intense JJ cord-104239-xxlcdbqi 406 16 morphological morphological JJ cord-104239-xxlcdbqi 406 17 specialization specialization NN cord-104239-xxlcdbqi 406 18 . . . cord-104239-xxlcdbqi 407 1 Each each DT cord-104239-xxlcdbqi 407 2 level level NN cord-104239-xxlcdbqi 407 3 of of IN cord-104239-xxlcdbqi 407 4 organization organization NN cord-104239-xxlcdbqi 407 5 is be VBZ cord-104239-xxlcdbqi 407 6 discussed discuss VBN cord-104239-xxlcdbqi 407 7 in in IN cord-104239-xxlcdbqi 407 8 detail detail NN cord-104239-xxlcdbqi 407 9 below below RB cord-104239-xxlcdbqi 407 10 . . . cord-104239-xxlcdbqi 408 1 While while IN cord-104239-xxlcdbqi 408 2 there there EX cord-104239-xxlcdbqi 408 3 is be VBZ cord-104239-xxlcdbqi 408 4 an an DT cord-104239-xxlcdbqi 408 5 apparent apparent JJ cord-104239-xxlcdbqi 408 6 random random JJ cord-104239-xxlcdbqi 408 7 distribution distribution NN cord-104239-xxlcdbqi 408 8 of of IN cord-104239-xxlcdbqi 408 9 export export NN cord-104239-xxlcdbqi 408 10 complexes complex NNS cord-104239-xxlcdbqi 408 11 in in IN cord-104239-xxlcdbqi 408 12 the the DT cord-104239-xxlcdbqi 408 13 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 408 14 , , , cord-104239-xxlcdbqi 408 15 we -PRON- PRP cord-104239-xxlcdbqi 408 16 found find VBD cord-104239-xxlcdbqi 408 17 a a DT cord-104239-xxlcdbqi 408 18 very very RB cord-104239-xxlcdbqi 408 19 high high JJ cord-104239-xxlcdbqi 408 20 degree degree NN cord-104239-xxlcdbqi 408 21 of of IN cord-104239-xxlcdbqi 408 22 organization organization NN cord-104239-xxlcdbqi 408 23 in in IN cord-104239-xxlcdbqi 408 24 the the DT cord-104239-xxlcdbqi 408 25 distribution distribution NN cord-104239-xxlcdbqi 408 26 of of IN cord-104239-xxlcdbqi 408 27 E e NN cord-104239-xxlcdbqi 408 28 R r NN cord-104239-xxlcdbqi 408 29 buds bud NNS cord-104239-xxlcdbqi 408 30 in in IN cord-104239-xxlcdbqi 408 31 the the DT cord-104239-xxlcdbqi 408 32 cell cell NN cord-104239-xxlcdbqi 408 33 . . . cord-104239-xxlcdbqi 409 1 We -PRON- PRP cord-104239-xxlcdbqi 409 2 observed observe VBD cord-104239-xxlcdbqi 409 3 not not RB cord-104239-xxlcdbqi 409 4 only only RB cord-104239-xxlcdbqi 409 5 a a DT cord-104239-xxlcdbqi 409 6 high high JJ cord-104239-xxlcdbqi 409 7 local local JJ cord-104239-xxlcdbqi 409 8 density density NN cord-104239-xxlcdbqi 409 9 on on IN cord-104239-xxlcdbqi 409 10 the the DT cord-104239-xxlcdbqi 409 11 same same JJ cord-104239-xxlcdbqi 409 12 stretch stretch NN cord-104239-xxlcdbqi 409 13 of of IN cord-104239-xxlcdbqi 409 14 E e NN cord-104239-xxlcdbqi 409 15 R r NN cord-104239-xxlcdbqi 409 16 membrane membrane NN cord-104239-xxlcdbqi 409 17 ( ( -LRB- cord-104239-xxlcdbqi 409 18 Fig Fig NNP cord-104239-xxlcdbqi 409 19 . . NNP cord-104239-xxlcdbqi 409 20 11 11 CD cord-104239-xxlcdbqi 409 21 , , , cord-104239-xxlcdbqi 409 22 tier tier NN cord-104239-xxlcdbqi 409 23 I I NNP cord-104239-xxlcdbqi 409 24 , , , cord-104239-xxlcdbqi 409 25 dotted dotted JJ cord-104239-xxlcdbqi 409 26 boxes box NNS cord-104239-xxlcdbqi 409 27 ) ) -RRB- cord-104239-xxlcdbqi 409 28 , , , cord-104239-xxlcdbqi 409 29 but but CC cord-104239-xxlcdbqi 409 30 found find VBD cord-104239-xxlcdbqi 409 31 distantly distantly RB cord-104239-xxlcdbqi 409 32 connected connect VBN cord-104239-xxlcdbqi 409 33 E e NN cord-104239-xxlcdbqi 409 34 R r NN cord-104239-xxlcdbqi 409 35 bud bud NN cord-104239-xxlcdbqi 409 36 - - HYPH cord-104239-xxlcdbqi 409 37 bearing bear VBG cord-104239-xxlcdbqi 409 38 zones zone NNS cord-104239-xxlcdbqi 409 39 encircling encircle VBG cord-104239-xxlcdbqi 409 40 the the DT cord-104239-xxlcdbqi 409 41 same same JJ cord-104239-xxlcdbqi 409 42 VTC vtc NN cord-104239-xxlcdbqi 409 43 ( ( -LRB- cord-104239-xxlcdbqi 409 44 Fig fig NN cord-104239-xxlcdbqi 409 45 . . NNP cord-104239-xxlcdbqi 409 46 11 11 CD cord-104239-xxlcdbqi 409 47 , , , cord-104239-xxlcdbqi 409 48 tier tier NN cord-104239-xxlcdbqi 409 49 II II NNP cord-104239-xxlcdbqi 409 50 , , , cord-104239-xxlcdbqi 409 51 dashed dash VBD cord-104239-xxlcdbqi 409 52 cylindrical cylindrical JJ cord-104239-xxlcdbqi 409 53 region region NN cord-104239-xxlcdbqi 409 54 ) ) -RRB- cord-104239-xxlcdbqi 409 55 , , , cord-104239-xxlcdbqi 409 56 suggestive suggestive JJ cord-104239-xxlcdbqi 409 57 of of IN cord-104239-xxlcdbqi 409 58 a a DT cord-104239-xxlcdbqi 409 59 regional regional JJ cord-104239-xxlcdbqi 409 60 specialization specialization NN cord-104239-xxlcdbqi 409 61 of of IN cord-104239-xxlcdbqi 409 62 the the DT cord-104239-xxlcdbqi 409 63 COPII COPII NNP cord-104239-xxlcdbqi 409 64 export export NN cord-104239-xxlcdbqi 409 65 machinery machinery NN cord-104239-xxlcdbqi 409 66 . . . cord-104239-xxlcdbqi 410 1 Consistent consistent JJ cord-104239-xxlcdbqi 410 2 with with IN cord-104239-xxlcdbqi 410 3 this this DT cord-104239-xxlcdbqi 410 4 interpretation interpretation NN cord-104239-xxlcdbqi 410 5 , , , cord-104239-xxlcdbqi 410 6 buds bud NNS cord-104239-xxlcdbqi 410 7 and and CC cord-104239-xxlcdbqi 410 8 vesicles vesicle NNS cord-104239-xxlcdbqi 410 9 accumulated accumulate VBN cord-104239-xxlcdbqi 410 10 in in IN cord-104239-xxlcdbqi 410 11 semi semi JJ cord-104239-xxlcdbqi 410 12 - - JJ cord-104239-xxlcdbqi 410 13 intact intact JJ cord-104239-xxlcdbqi 410 14 cells cell NNS cord-104239-xxlcdbqi 410 15 in in IN cord-104239-xxlcdbqi 410 16 the the DT cord-104239-xxlcdbqi 410 17 pres pres NNP cord-104239-xxlcdbqi 410 18 - - HYPH cord-104239-xxlcdbqi 410 19 ence ence NNP cord-104239-xxlcdbqi 410 20 of of IN cord-104239-xxlcdbqi 410 21 GTP~/S GTP~/S NNP cord-104239-xxlcdbqi 410 22 or or CC cord-104239-xxlcdbqi 410 23 the the DT cord-104239-xxlcdbqi 410 24 Sar1[H79 Sar1[H79 NNP cord-104239-xxlcdbqi 410 25 G G NNP cord-104239-xxlcdbqi 410 26 ] ] -RRB- cord-104239-xxlcdbqi 410 27 mutant mutant NN cord-104239-xxlcdbqi 410 28 strongly strongly RB cord-104239-xxlcdbqi 410 29 labeled label VBN cord-104239-xxlcdbqi 410 30 with with IN cord-104239-xxlcdbqi 410 31 antibodies antibody NNS cord-104239-xxlcdbqi 410 32 specific specific JJ cord-104239-xxlcdbqi 410 33 for for IN cord-104239-xxlcdbqi 410 34 mammalian mammalian JJ cord-104239-xxlcdbqi 410 35 homologues homologue NNS cord-104239-xxlcdbqi 410 36 of of IN cord-104239-xxlcdbqi 410 37 the the DT cord-104239-xxlcdbqi 410 38 yeast yeast NN cord-104239-xxlcdbqi 410 39 components component NNS cord-104239-xxlcdbqi 410 40 Secl3p Secl3p NNP cord-104239-xxlcdbqi 410 41 and and CC cord-104239-xxlcdbqi 410 42 Sec23p Sec23p NNP cord-104239-xxlcdbqi 410 43 . . . cord-104239-xxlcdbqi 411 1 Both both DT cord-104239-xxlcdbqi 411 2 the the DT cord-104239-xxlcdbqi 411 3 GTP GTP NNP cord-104239-xxlcdbqi 411 4 - - HYPH cord-104239-xxlcdbqi 411 5 yS yS NNP cord-104239-xxlcdbqi 411 6 and and CC cord-104239-xxlcdbqi 411 7 the the DT cord-104239-xxlcdbqi 411 8 GTP GTP NNP cord-104239-xxlcdbqi 411 9 - - HYPH cord-104239-xxlcdbqi 411 10 restricted restrict VBN cord-104239-xxlcdbqi 411 11 Sarl[H79 Sarl[H79 NNP cord-104239-xxlcdbqi 411 12 G g NN cord-104239-xxlcdbqi 411 13 ] ] -RRB- cord-104239-xxlcdbqi 411 14 mutant mutant NN cord-104239-xxlcdbqi 411 15 led lead VBD cord-104239-xxlcdbqi 411 16 to to IN cord-104239-xxlcdbqi 411 17 the the DT cord-104239-xxlcdbqi 411 18 formation formation NN cord-104239-xxlcdbqi 411 19 in in IN cord-104239-xxlcdbqi 411 20 vitro vitro FW cord-104239-xxlcdbqi 411 21 of of IN cord-104239-xxlcdbqi 411 22 ER er NN cord-104239-xxlcdbqi 411 23 - - HYPH cord-104239-xxlcdbqi 411 24 derived derive VBN cord-104239-xxlcdbqi 411 25 vesicle vesicle NN cord-104239-xxlcdbqi 411 26 clusters cluster NNS cord-104239-xxlcdbqi 411 27 and and CC cord-104239-xxlcdbqi 411 28 strings string NNS cord-104239-xxlcdbqi 411 29 of of IN cord-104239-xxlcdbqi 411 30 vesicles vesicle NNS cord-104239-xxlcdbqi 411 31 with with IN cord-104239-xxlcdbqi 411 32 a a DT cord-104239-xxlcdbqi 411 33 necklace necklace NN cord-104239-xxlcdbqi 411 34 - - HYPH cord-104239-xxlcdbqi 411 35 like like JJ cord-104239-xxlcdbqi 411 36 appearance appearance NN cord-104239-xxlcdbqi 411 37 . . . cord-104239-xxlcdbqi 412 1 Vesicles Vesicles NNP cord-104239-xxlcdbqi 412 2 accumulated accumulate VBN cord-104239-xxlcdbqi 412 3 as as IN cord-104239-xxlcdbqi 412 4 necklaces necklace NNS cord-104239-xxlcdbqi 412 5 had have VBD cord-104239-xxlcdbqi 412 6 no no DT cord-104239-xxlcdbqi 412 7 obvious obvious JJ cord-104239-xxlcdbqi 412 8 continuity continuity NN cord-104239-xxlcdbqi 412 9 between between IN cord-104239-xxlcdbqi 412 10 the the DT cord-104239-xxlcdbqi 412 11 lumen lumen NN cord-104239-xxlcdbqi 412 12 of of IN cord-104239-xxlcdbqi 412 13 individual individual JJ cord-104239-xxlcdbqi 412 14 vesicles vesicle NNS cord-104239-xxlcdbqi 412 15 , , , cord-104239-xxlcdbqi 412 16 suggesting suggest VBG cord-104239-xxlcdbqi 412 17 that that IN cord-104239-xxlcdbqi 412 18 membrane membrane NN cord-104239-xxlcdbqi 412 19 fission fission NN cord-104239-xxlcdbqi 412 20 had have VBD cord-104239-xxlcdbqi 412 21 gone go VBN cord-104239-xxlcdbqi 412 22 to to IN cord-104239-xxlcdbqi 412 23 completion completion NN cord-104239-xxlcdbqi 412 24 . . . cord-104239-xxlcdbqi 413 1 Moreover moreover RB cord-104239-xxlcdbqi 413 2 , , , cord-104239-xxlcdbqi 413 3 in in IN cord-104239-xxlcdbqi 413 4 both both DT cord-104239-xxlcdbqi 413 5 thin thin JJ cord-104239-xxlcdbqi 413 6 - - HYPH cord-104239-xxlcdbqi 413 7 section section NN cord-104239-xxlcdbqi 413 8 and and CC cord-104239-xxlcdbqi 413 9 replicas replica NNS cord-104239-xxlcdbqi 413 10 , , , cord-104239-xxlcdbqi 413 11 clusters cluster NNS cord-104239-xxlcdbqi 413 12 that that WDT cord-104239-xxlcdbqi 413 13 formed form VBD cord-104239-xxlcdbqi 413 14 in in IN cord-104239-xxlcdbqi 413 15 the the DT cord-104239-xxlcdbqi 413 16 presence presence NN cord-104239-xxlcdbqi 413 17 of of IN cord-104239-xxlcdbqi 413 18 GTP'yS gtp'ys CD cord-104239-xxlcdbqi 413 19 or or CC cord-104239-xxlcdbqi 413 20 the the DT cord-104239-xxlcdbqi 413 21 Sarl Sarl NNP cord-104239-xxlcdbqi 413 22 GTPrestricted gtprestricte VBN cord-104239-xxlcdbqi 413 23 mutant mutant NN cord-104239-xxlcdbqi 413 24 clearly clearly RB cord-104239-xxlcdbqi 413 25 had have VBD cord-104239-xxlcdbqi 413 26 a a DT cord-104239-xxlcdbqi 413 27 more more RBR cord-104239-xxlcdbqi 413 28 vesicular vesicular JJ cord-104239-xxlcdbqi 413 29 surface surface NN cord-104239-xxlcdbqi 413 30 appearance appearance NN cord-104239-xxlcdbqi 413 31 than than IN cord-104239-xxlcdbqi 413 32 those those DT cord-104239-xxlcdbqi 413 33 formed form VBN cord-104239-xxlcdbqi 413 34 in in IN cord-104239-xxlcdbqi 413 35 the the DT cord-104239-xxlcdbqi 413 36 absence absence NN cord-104239-xxlcdbqi 413 37 of of IN cord-104239-xxlcdbqi 413 38 inhibitors inhibitor NNS cord-104239-xxlcdbqi 413 39 . . . cord-104239-xxlcdbqi 414 1 The the DT cord-104239-xxlcdbqi 414 2 fact fact NN cord-104239-xxlcdbqi 414 3 that that IN cord-104239-xxlcdbqi 414 4 separation separation NN cord-104239-xxlcdbqi 414 5 of of IN cord-104239-xxlcdbqi 414 6 vesicles vesicle NNS cord-104239-xxlcdbqi 414 7 appears appear VBZ cord-104239-xxlcdbqi 414 8 to to TO cord-104239-xxlcdbqi 414 9 be be VB cord-104239-xxlcdbqi 414 10 blocked block VBN cord-104239-xxlcdbqi 414 11 in in IN cord-104239-xxlcdbqi 414 12 the the DT cord-104239-xxlcdbqi 414 13 absence absence NN cord-104239-xxlcdbqi 414 14 of of IN cord-104239-xxlcdbqi 414 15 GTP gtp NN cord-104239-xxlcdbqi 414 16 hydrolysis hydrolysis NN cord-104239-xxlcdbqi 414 17 raises raise VBZ cord-104239-xxlcdbqi 414 18 the the DT cord-104239-xxlcdbqi 414 19 distinct distinct JJ cord-104239-xxlcdbqi 414 20 possibly possibly RB cord-104239-xxlcdbqi 414 21 that that IN cord-104239-xxlcdbqi 414 22 the the DT cord-104239-xxlcdbqi 414 23 function function NN cord-104239-xxlcdbqi 414 24 of of IN cord-104239-xxlcdbqi 414 25 Sarl Sarl NNP cord-104239-xxlcdbqi 414 26 is be VBZ cord-104239-xxlcdbqi 414 27 normally normally RB cord-104239-xxlcdbqi 414 28 required require VBN cord-104239-xxlcdbqi 414 29 for for IN cord-104239-xxlcdbqi 414 30 this this DT cord-104239-xxlcdbqi 414 31 event event NN cord-104239-xxlcdbqi 414 32 . . . cord-104239-xxlcdbqi 415 1 Given give VBN cord-104239-xxlcdbqi 415 2 the the DT cord-104239-xxlcdbqi 415 3 fact fact NN cord-104239-xxlcdbqi 415 4 that that IN cord-104239-xxlcdbqi 415 5 uncoating uncoating NN cord-104239-xxlcdbqi 415 6 occurs occur VBZ cord-104239-xxlcdbqi 415 7 rapidly rapidly RB cord-104239-xxlcdbqi 415 8 after after IN cord-104239-xxlcdbqi 415 9 budding bud VBG cord-104239-xxlcdbqi 415 10 ( ( -LRB- cord-104239-xxlcdbqi 415 11 Aridor Aridor NNP cord-104239-xxlcdbqi 415 12 et et NNP cord-104239-xxlcdbqi 415 13 al al NNP cord-104239-xxlcdbqi 415 14 . . NNP cord-104239-xxlcdbqi 415 15 , , , cord-104239-xxlcdbqi 415 16 1995 1995 CD cord-104239-xxlcdbqi 415 17 ) ) -RRB- cord-104239-xxlcdbqi 415 18 , , , cord-104239-xxlcdbqi 415 19 coat coat NN cord-104239-xxlcdbqi 415 20 disassembly disassembly NN cord-104239-xxlcdbqi 415 21 could could MD cord-104239-xxlcdbqi 415 22 be be VB cord-104239-xxlcdbqi 415 23 associated associate VBN cord-104239-xxlcdbqi 415 24 with with IN cord-104239-xxlcdbqi 415 25 release release NN cord-104239-xxlcdbqi 415 26 of of IN cord-104239-xxlcdbqi 415 27 vesicles vesicle NNS cord-104239-xxlcdbqi 415 28 from from IN cord-104239-xxlcdbqi 415 29 necklaces necklace NNS cord-104239-xxlcdbqi 415 30 . . . cord-104239-xxlcdbqi 416 1 The the DT cord-104239-xxlcdbqi 416 2 morphological morphological JJ cord-104239-xxlcdbqi 416 3 effect effect NN cord-104239-xxlcdbqi 416 4 of of IN cord-104239-xxlcdbqi 416 5 GTP GTP NNP cord-104239-xxlcdbqi 416 6 ~ ~ NFP cord-104239-xxlcdbqi 416 7 S S NNP cord-104239-xxlcdbqi 416 8 on on IN cord-104239-xxlcdbqi 416 9 the the DT cord-104239-xxlcdbqi 416 10 budding budding NN cord-104239-xxlcdbqi 416 11 from from IN cord-104239-xxlcdbqi 416 12 the the DT cord-104239-xxlcdbqi 416 13 E e NN cord-104239-xxlcdbqi 416 14 R r NN cord-104239-xxlcdbqi 416 15 is be VBZ cord-104239-xxlcdbqi 416 16 very very RB cord-104239-xxlcdbqi 416 17 different different JJ cord-104239-xxlcdbqi 416 18 from from IN cord-104239-xxlcdbqi 416 19 its -PRON- PRP$ cord-104239-xxlcdbqi 416 20 effect effect NN cord-104239-xxlcdbqi 416 21 on on IN cord-104239-xxlcdbqi 416 22 Golgi Golgi NNP cord-104239-xxlcdbqi 416 23 membranes membrane NNS cord-104239-xxlcdbqi 416 24 incubated incubate VBN cord-104239-xxlcdbqi 416 25 under under IN cord-104239-xxlcdbqi 416 26 identical identical JJ cord-104239-xxlcdbqi 416 27 conditions condition NNS cord-104239-xxlcdbqi 416 28 . . . cord-104239-xxlcdbqi 417 1 In in IN cord-104239-xxlcdbqi 417 2 the the DT cord-104239-xxlcdbqi 417 3 latter latter JJ cord-104239-xxlcdbqi 417 4 case case NN cord-104239-xxlcdbqi 417 5 , , , cord-104239-xxlcdbqi 417 6 buds bud NNS cord-104239-xxlcdbqi 417 7 appearing appear VBG cord-104239-xxlcdbqi 417 8 in in IN cord-104239-xxlcdbqi 417 9 replicas replica NNS cord-104239-xxlcdbqi 417 10 are be VBP cord-104239-xxlcdbqi 417 11 isolated isolate VBN cord-104239-xxlcdbqi 417 12 , , , cord-104239-xxlcdbqi 417 13 single single JJ cord-104239-xxlcdbqi 417 14 structures structure NNS cord-104239-xxlcdbqi 417 15 and and CC cord-104239-xxlcdbqi 417 16 are be VBP cord-104239-xxlcdbqi 417 17 uniformly uniformly RB cord-104239-xxlcdbqi 417 18 distributed distribute VBN cord-104239-xxlcdbqi 417 19 throughout throughout IN cord-104239-xxlcdbqi 417 20 the the DT cord-104239-xxlcdbqi 417 21 entire entire JJ cord-104239-xxlcdbqi 417 22 Golgi Golgi NNP cord-104239-xxlcdbqi 417 23 surface surface NN cord-104239-xxlcdbqi 417 24 ( ( -LRB- cord-104239-xxlcdbqi 417 25 Weidman Weidman NNP cord-104239-xxlcdbqi 417 26 et et NNP cord-104239-xxlcdbqi 417 27 al al NNP cord-104239-xxlcdbqi 417 28 . . NNP cord-104239-xxlcdbqi 417 29 , , , cord-104239-xxlcdbqi 417 30 1993 1993 CD cord-104239-xxlcdbqi 417 31 ) ) -RRB- cord-104239-xxlcdbqi 417 32 . . . cord-104239-xxlcdbqi 418 1 The the DT cord-104239-xxlcdbqi 418 2 regional regional JJ cord-104239-xxlcdbqi 418 3 confinement confinement NN cord-104239-xxlcdbqi 418 4 of of IN cord-104239-xxlcdbqi 418 5 budding bud VBG cord-104239-xxlcdbqi 418 6 in in IN cord-104239-xxlcdbqi 418 7 E e NN cord-104239-xxlcdbqi 418 8 R r NN cord-104239-xxlcdbqi 418 9 membranes membrane NNS cord-104239-xxlcdbqi 418 10 to to IN cord-104239-xxlcdbqi 418 11 necklaces necklace NNS cord-104239-xxlcdbqi 418 12 or or CC cord-104239-xxlcdbqi 418 13 clusters cluster NNS cord-104239-xxlcdbqi 418 14 therefore therefore RB cord-104239-xxlcdbqi 418 15 also also RB cord-104239-xxlcdbqi 418 16 supports support VBZ cord-104239-xxlcdbqi 418 17 our -PRON- PRP$ cord-104239-xxlcdbqi 418 18 interpretation interpretation NN cord-104239-xxlcdbqi 418 19 that that IN cord-104239-xxlcdbqi 418 20 export export NN cord-104239-xxlcdbqi 418 21 occurs occur VBZ cord-104239-xxlcdbqi 418 22 from from IN cord-104239-xxlcdbqi 418 23 areas area NNS cord-104239-xxlcdbqi 418 24 of of IN cord-104239-xxlcdbqi 418 25 luminal luminal NN cord-104239-xxlcdbqi 418 26 and/or and/or CC cord-104239-xxlcdbqi 418 27 membrane membrane NN cord-104239-xxlcdbqi 418 28 specialization specialization NN cord-104239-xxlcdbqi 418 29 . . . cord-104239-xxlcdbqi 419 1 Since since IN cord-104239-xxlcdbqi 419 2 budding bud VBG cord-104239-xxlcdbqi 419 3 from from IN cord-104239-xxlcdbqi 419 4 the the DT cord-104239-xxlcdbqi 419 5 E e NN cord-104239-xxlcdbqi 419 6 R r NN cord-104239-xxlcdbqi 419 7 frequently frequently RB cord-104239-xxlcdbqi 419 8 occurs occur VBZ cord-104239-xxlcdbqi 419 9 from from IN cord-104239-xxlcdbqi 419 10 the the DT cord-104239-xxlcdbqi 419 11 tips tip NNS cord-104239-xxlcdbqi 419 12 of of IN cord-104239-xxlcdbqi 419 13 short short JJ cord-104239-xxlcdbqi 419 14 , , , cord-104239-xxlcdbqi 419 15 coated coated JJ cord-104239-xxlcdbqi 419 16 tubules tubule NNS cord-104239-xxlcdbqi 419 17 emanating emanate VBG cord-104239-xxlcdbqi 419 18 from from IN cord-104239-xxlcdbqi 419 19 E E NNP cord-104239-xxlcdbqi 419 20 R R NNP cord-104239-xxlcdbqi 419 21 cisternae cisternae NN cord-104239-xxlcdbqi 419 22 , , , cord-104239-xxlcdbqi 419 23 clusters cluster NNS cord-104239-xxlcdbqi 419 24 and and CC cord-104239-xxlcdbqi 419 25 necklaces necklace NNS cord-104239-xxlcdbqi 419 26 could could MD cord-104239-xxlcdbqi 419 27 be be VB cord-104239-xxlcdbqi 419 28 derived derive VBN cord-104239-xxlcdbqi 419 29 from from IN cord-104239-xxlcdbqi 419 30 either either DT cord-104239-xxlcdbqi 419 31 sequential sequential JJ cord-104239-xxlcdbqi 419 32 or or CC cord-104239-xxlcdbqi 419 33 synchronous synchronous JJ cord-104239-xxlcdbqi 419 34 fission fission NN cord-104239-xxlcdbqi 419 35 of of IN cord-104239-xxlcdbqi 419 36 these these DT cord-104239-xxlcdbqi 419 37 tubular tubular JJ cord-104239-xxlcdbqi 419 38 elements element NNS cord-104239-xxlcdbqi 419 39 . . . cord-104239-xxlcdbqi 420 1 In in IN cord-104239-xxlcdbqi 420 2 contrast contrast NN cord-104239-xxlcdbqi 420 3 with with IN cord-104239-xxlcdbqi 420 4 the the DT cord-104239-xxlcdbqi 420 5 fact fact NN cord-104239-xxlcdbqi 420 6 that that IN cord-104239-xxlcdbqi 420 7 export export NN cord-104239-xxlcdbqi 420 8 complexes complex NNS cord-104239-xxlcdbqi 420 9 observed observe VBN cord-104239-xxlcdbqi 420 10 in in IN cord-104239-xxlcdbqi 420 11 vivo vivo NN cord-104239-xxlcdbqi 420 12 appear appear VBP cord-104239-xxlcdbqi 420 13 to to TO cord-104239-xxlcdbqi 420 14 be be VB cord-104239-xxlcdbqi 420 15 completely completely RB cord-104239-xxlcdbqi 420 16 surrounded surround VBN cord-104239-xxlcdbqi 420 17 by by IN cord-104239-xxlcdbqi 420 18 budding bud VBG cord-104239-xxlcdbqi 420 19 profiles profile NNS cord-104239-xxlcdbqi 420 20 from from IN cord-104239-xxlcdbqi 420 21 topologically topologically RB cord-104239-xxlcdbqi 420 22 distant distant JJ cord-104239-xxlcdbqi 420 23 E e NN cord-104239-xxlcdbqi 420 24 R r NN cord-104239-xxlcdbqi 420 25 cisternae cisternae NN cord-104239-xxlcdbqi 420 26 ( ( -LRB- cord-104239-xxlcdbqi 420 27 Fig fig NN cord-104239-xxlcdbqi 420 28 . . . cord-104239-xxlcdbqi 421 1 11 11 CD cord-104239-xxlcdbqi 421 2 A A NNP cord-104239-xxlcdbqi 421 3 , , , cord-104239-xxlcdbqi 421 4 tier tier NN cord-104239-xxlcdbqi 421 5 II II NNP cord-104239-xxlcdbqi 421 6 ) ) -RRB- cord-104239-xxlcdbqi 421 7 , , , cord-104239-xxlcdbqi 421 8 semi semi JJ cord-104239-xxlcdbqi 421 9 - - JJ cord-104239-xxlcdbqi 421 10 intact intact JJ cord-104239-xxlcdbqi 421 11 cells cell NNS cord-104239-xxlcdbqi 421 12 lacked lack VBD cord-104239-xxlcdbqi 421 13 this this DT cord-104239-xxlcdbqi 421 14 feature feature NN cord-104239-xxlcdbqi 421 15 ( ( -LRB- cord-104239-xxlcdbqi 421 16 Fig Fig NNP cord-104239-xxlcdbqi 421 17 . . . cord-104239-xxlcdbqi 421 18 11 11 CD cord-104239-xxlcdbqi 421 19 B b NN cord-104239-xxlcdbqi 421 20 ) ) -RRB- cord-104239-xxlcdbqi 421 21 . . . cord-104239-xxlcdbqi 422 1 Thus thus RB cord-104239-xxlcdbqi 422 2 , , , cord-104239-xxlcdbqi 422 3 permeabilization permeabilization NN cord-104239-xxlcdbqi 422 4 destroys destroy VBZ cord-104239-xxlcdbqi 422 5 confinement confinement NN cord-104239-xxlcdbqi 422 6 of of IN cord-104239-xxlcdbqi 422 7 several several JJ cord-104239-xxlcdbqi 422 8 sites site NNS cord-104239-xxlcdbqi 422 9 to to IN cord-104239-xxlcdbqi 422 10 one one CD cord-104239-xxlcdbqi 422 11 area area NN cord-104239-xxlcdbqi 422 12 and and CC cord-104239-xxlcdbqi 422 13 allows allow VBZ cord-104239-xxlcdbqi 422 14 them -PRON- PRP cord-104239-xxlcdbqi 422 15 to to TO cord-104239-xxlcdbqi 422 16 form form VB cord-104239-xxlcdbqi 422 17 in in IN cord-104239-xxlcdbqi 422 18 a a DT cord-104239-xxlcdbqi 422 19 more more RBR cord-104239-xxlcdbqi 422 20 random random JJ cord-104239-xxlcdbqi 422 21 fashion fashion NN cord-104239-xxlcdbqi 422 22 . . . cord-104239-xxlcdbqi 423 1 We -PRON- PRP cord-104239-xxlcdbqi 423 2 have have VBP cord-104239-xxlcdbqi 423 3 previously previously RB cord-104239-xxlcdbqi 423 4 noted note VBN cord-104239-xxlcdbqi 423 5 that that IN cord-104239-xxlcdbqi 423 6 ER er NN cord-104239-xxlcdbqi 423 7 - - HYPH cord-104239-xxlcdbqi 423 8 derived derive VBN cord-104239-xxlcdbqi 423 9 vesicles vesicle NNS cord-104239-xxlcdbqi 423 10 and and CC cord-104239-xxlcdbqi 423 11 downstream downstream JJ cord-104239-xxlcdbqi 423 12 compartments compartment NNS cord-104239-xxlcdbqi 423 13 are be VBP cord-104239-xxlcdbqi 423 14 not not RB cord-104239-xxlcdbqi 423 15 released release VBN cord-104239-xxlcdbqi 423 16 from from IN cord-104239-xxlcdbqi 423 17 semi semi JJ cord-104239-xxlcdbqi 423 18 - - JJ cord-104239-xxlcdbqi 423 19 intact intact JJ cord-104239-xxlcdbqi 423 20 cells cell NNS cord-104239-xxlcdbqi 423 21 during during IN cord-104239-xxlcdbqi 423 22 incubation incubation NN cord-104239-xxlcdbqi 423 23 in in IN cord-104239-xxlcdbqi 423 24 vitro vitro FW cord-104239-xxlcdbqi 423 25 at at IN cord-104239-xxlcdbqi 423 26 32 32 CD cord-104239-xxlcdbqi 423 27 ° ° NN cord-104239-xxlcdbqi 423 28 C c NN cord-104239-xxlcdbqi 423 29 ( ( -LRB- cord-104239-xxlcdbqi 423 30 Beckers Beckers NNP cord-104239-xxlcdbqi 423 31 et et NNP cord-104239-xxlcdbqi 423 32 al al NNP cord-104239-xxlcdbqi 423 33 . . NNP cord-104239-xxlcdbqi 423 34 , , , cord-104239-xxlcdbqi 423 35 1987 1987 CD cord-104239-xxlcdbqi 423 36 ) ) -RRB- cord-104239-xxlcdbqi 423 37 . . . cord-104239-xxlcdbqi 424 1 In in IN cord-104239-xxlcdbqi 424 2 contrast contrast NN cord-104239-xxlcdbqi 424 3 , , , cord-104239-xxlcdbqi 424 4 assays assay NNS cord-104239-xxlcdbqi 424 5 that that WDT cord-104239-xxlcdbqi 424 6 reconstitute reconstitute VBP cord-104239-xxlcdbqi 424 7 vesicle vesicle NN cord-104239-xxlcdbqi 424 8 budding bud VBG cord-104239-xxlcdbqi 424 9 from from IN cord-104239-xxlcdbqi 424 10 semi semi JJ cord-104239-xxlcdbqi 424 11 - - JJ cord-104239-xxlcdbqi 424 12 intact intact JJ cord-104239-xxlcdbqi 424 13 yeast yeast NN cord-104239-xxlcdbqi 424 14 cells cell NNS cord-104239-xxlcdbqi 425 1 ( ( -LRB- cord-104239-xxlcdbqi 425 2 Baker Baker NNP cord-104239-xxlcdbqi 425 3 et et NNP cord-104239-xxlcdbqi 425 4 al al NNP cord-104239-xxlcdbqi 425 5 . . NNP cord-104239-xxlcdbqi 425 6 , , , cord-104239-xxlcdbqi 425 7 1988 1988 CD cord-104239-xxlcdbqi 425 8 ) ) -RRB- cord-104239-xxlcdbqi 425 9 release release VBP cord-104239-xxlcdbqi 425 10 free free JJ cord-104239-xxlcdbqi 425 11 60-nm 60-nm CD cord-104239-xxlcdbqi 425 12 COPII COPII NNP cord-104239-xxlcdbqi 425 13 - - HYPH cord-104239-xxlcdbqi 425 14 coated coat VBN cord-104239-xxlcdbqi 425 15 vesicular vesicular JJ cord-104239-xxlcdbqi 425 16 carriers carrier NNS cord-104239-xxlcdbqi 425 17 ( ( -LRB- cord-104239-xxlcdbqi 425 18 Barlowe Barlowe NNP cord-104239-xxlcdbqi 425 19 et et FW cord-104239-xxlcdbqi 425 20 al al NNP cord-104239-xxlcdbqi 425 21 . . NNP cord-104239-xxlcdbqi 425 22 , , , cord-104239-xxlcdbqi 425 23 1994 1994 CD cord-104239-xxlcdbqi 425 24 ) ) -RRB- cord-104239-xxlcdbqi 425 25 . . . cord-104239-xxlcdbqi 426 1 The the DT cord-104239-xxlcdbqi 426 2 inability inability NN cord-104239-xxlcdbqi 426 3 of of IN cord-104239-xxlcdbqi 426 4 mammalian mammalian JJ cord-104239-xxlcdbqi 426 5 semiintact semiintact NN cord-104239-xxlcdbqi 426 6 cells cell NNS cord-104239-xxlcdbqi 426 7 to to TO cord-104239-xxlcdbqi 426 8 release release VB cord-104239-xxlcdbqi 426 9 vesicles vesicle NNS cord-104239-xxlcdbqi 426 10 suggests suggest VBZ cord-104239-xxlcdbqi 426 11 that that IN cord-104239-xxlcdbqi 426 12 vesicles vesicle NNS cord-104239-xxlcdbqi 426 13 are be VBP cord-104239-xxlcdbqi 426 14 tethered tether VBN cord-104239-xxlcdbqi 426 15 to to IN cord-104239-xxlcdbqi 426 16 a a DT cord-104239-xxlcdbqi 426 17 scaffold scaffold NN cord-104239-xxlcdbqi 426 18 of of IN cord-104239-xxlcdbqi 426 19 unknown unknown JJ cord-104239-xxlcdbqi 426 20 composition composition NN cord-104239-xxlcdbqi 426 21 . . . cord-104239-xxlcdbqi 427 1 In in IN cord-104239-xxlcdbqi 427 2 yeast yeast NN cord-104239-xxlcdbqi 427 3 , , , cord-104239-xxlcdbqi 427 4 either either CC cord-104239-xxlcdbqi 427 5 vesicles vesicle NNS cord-104239-xxlcdbqi 427 6 are be VBP cord-104239-xxlcdbqi 427 7 not not RB cord-104239-xxlcdbqi 427 8 linked link VBN cord-104239-xxlcdbqi 427 9 to to IN cord-104239-xxlcdbqi 427 10 such such PDT cord-104239-xxlcdbqi 427 11 a a DT cord-104239-xxlcdbqi 427 12 scaffold scaffold NN cord-104239-xxlcdbqi 427 13 , , , cord-104239-xxlcdbqi 427 14 or or CC cord-104239-xxlcdbqi 427 15 this this DT cord-104239-xxlcdbqi 427 16 aspect aspect NN cord-104239-xxlcdbqi 427 17 has have VBZ cord-104239-xxlcdbqi 427 18 not not RB cord-104239-xxlcdbqi 427 19 been be VBN cord-104239-xxlcdbqi 427 20 successfully successfully RB cord-104239-xxlcdbqi 427 21 reconstituted reconstitute VBN cord-104239-xxlcdbqi 427 22 . . . cord-104239-xxlcdbqi 428 1 In in IN cord-104239-xxlcdbqi 428 2 either either DT cord-104239-xxlcdbqi 428 3 event event NN cord-104239-xxlcdbqi 428 4 , , , cord-104239-xxlcdbqi 428 5 while while IN cord-104239-xxlcdbqi 428 6 the the DT cord-104239-xxlcdbqi 428 7 striking striking JJ cord-104239-xxlcdbqi 428 8 degree degree NN cord-104239-xxlcdbqi 428 9 of of IN cord-104239-xxlcdbqi 428 10 morphological morphological JJ cord-104239-xxlcdbqi 428 11 specialization specialization NN cord-104239-xxlcdbqi 428 12 observed observe VBN cord-104239-xxlcdbqi 428 13 in in IN cord-104239-xxlcdbqi 428 14 mammalian mammalian JJ cord-104239-xxlcdbqi 428 15 cells cell NNS cord-104239-xxlcdbqi 428 16 may may MD cord-104239-xxlcdbqi 428 17 contribute contribute VB cord-104239-xxlcdbqi 428 18 to to IN cord-104239-xxlcdbqi 428 19 the the DT cord-104239-xxlcdbqi 428 20 overall overall JJ cord-104239-xxlcdbqi 428 21 efficiency efficiency NN cord-104239-xxlcdbqi 428 22 of of IN cord-104239-xxlcdbqi 428 23 budding budding NN cord-104239-xxlcdbqi 428 24 and and CC cord-104239-xxlcdbqi 428 25 transport transport NN cord-104239-xxlcdbqi 428 26 in in IN cord-104239-xxlcdbqi 428 27 the the DT cord-104239-xxlcdbqi 428 28 early early JJ cord-104239-xxlcdbqi 428 29 secretory secretory JJ cord-104239-xxlcdbqi 428 30 pathway pathway NN cord-104239-xxlcdbqi 428 31 , , , cord-104239-xxlcdbqi 428 32 it -PRON- PRP cord-104239-xxlcdbqi 428 33 is be VBZ cord-104239-xxlcdbqi 428 34 apparently apparently RB cord-104239-xxlcdbqi 428 35 not not RB cord-104239-xxlcdbqi 428 36 essential essential JJ cord-104239-xxlcdbqi 428 37 . . . cord-104239-xxlcdbqi 429 1 We -PRON- PRP cord-104239-xxlcdbqi 429 2 have have VBP cord-104239-xxlcdbqi 429 3 previously previously RB cord-104239-xxlcdbqi 429 4 suggested suggest VBN cord-104239-xxlcdbqi 429 5 that that IN cord-104239-xxlcdbqi 429 6 export export NN cord-104239-xxlcdbqi 429 7 from from IN cord-104239-xxlcdbqi 429 8 the the DT cord-104239-xxlcdbqi 429 9 E e NN cord-104239-xxlcdbqi 429 10 R r NN cord-104239-xxlcdbqi 429 11 is be VBZ cord-104239-xxlcdbqi 429 12 accompanied accompany VBN cord-104239-xxlcdbqi 429 13 by by IN cord-104239-xxlcdbqi 429 14 concentration concentration NN cord-104239-xxlcdbqi 429 15 of of IN cord-104239-xxlcdbqi 429 16 VSV VSV NNP cord-104239-xxlcdbqi 429 17 - - HYPH cord-104239-xxlcdbqi 429 18 G G NNP cord-104239-xxlcdbqi 429 19 . . . cord-104239-xxlcdbqi 430 1 This this DT cord-104239-xxlcdbqi 430 2 point point NN cord-104239-xxlcdbqi 430 3 was be VBD cord-104239-xxlcdbqi 430 4 the the DT cord-104239-xxlcdbqi 430 5 subject subject NN cord-104239-xxlcdbqi 430 6 of of IN cord-104239-xxlcdbqi 430 7 a a DT cord-104239-xxlcdbqi 430 8 recent recent JJ cord-104239-xxlcdbqi 430 9 debate debate NN cord-104239-xxlcdbqi 430 10 ( ( -LRB- cord-104239-xxlcdbqi 430 11 Balch Balch NNP cord-104239-xxlcdbqi 430 12 and and CC cord-104239-xxlcdbqi 430 13 Farquhar Farquhar NNP cord-104239-xxlcdbqi 430 14 , , , cord-104239-xxlcdbqi 430 15 1995 1995 CD cord-104239-xxlcdbqi 430 16 ; ; : cord-104239-xxlcdbqi 430 17 Griffiths Griffiths NNP cord-104239-xxlcdbqi 430 18 et et NNP cord-104239-xxlcdbqi 430 19 al al NNP cord-104239-xxlcdbqi 430 20 . . NNP cord-104239-xxlcdbqi 430 21 , , , cord-104239-xxlcdbqi 430 22 1995a 1995a CD cord-104239-xxlcdbqi 430 23 ) ) -RRB- cord-104239-xxlcdbqi 430 24 . . . cord-104239-xxlcdbqi 431 1 We -PRON- PRP cord-104239-xxlcdbqi 431 2 have have VBP cord-104239-xxlcdbqi 431 3 now now RB cord-104239-xxlcdbqi 431 4 applied apply VBN cord-104239-xxlcdbqi 431 5 an an DT cord-104239-xxlcdbqi 431 6 independent independent JJ cord-104239-xxlcdbqi 431 7 approach approach NN cord-104239-xxlcdbqi 431 8 using use VBG cord-104239-xxlcdbqi 431 9 quick quick JJ cord-104239-xxlcdbqi 431 10 - - HYPH cord-104239-xxlcdbqi 431 11 freeze freeze NN cord-104239-xxlcdbqi 431 12 , , , cord-104239-xxlcdbqi 431 13 deepetch deepetch NN cord-104239-xxlcdbqi 431 14 methodologies methodology NNS cord-104239-xxlcdbqi 431 15 in in IN cord-104239-xxlcdbqi 431 16 conjunction conjunction NN cord-104239-xxlcdbqi 431 17 with with IN cord-104239-xxlcdbqi 431 18 immunolabeling immunolabele VBG cord-104239-xxlcdbqi 431 19 of of IN cord-104239-xxlcdbqi 431 20 VSV VSV NNP cord-104239-xxlcdbqi 431 21 - - HYPH cord-104239-xxlcdbqi 431 22 G G NNP cord-104239-xxlcdbqi 431 23 to to TO cord-104239-xxlcdbqi 431 24 generate generate VB cord-104239-xxlcdbqi 431 25 three three CD cord-104239-xxlcdbqi 431 26 - - HYPH cord-104239-xxlcdbqi 431 27 dimensional dimensional JJ cord-104239-xxlcdbqi 431 28 replicas replica NNS cord-104239-xxlcdbqi 431 29 that that WDT cord-104239-xxlcdbqi 431 30 allow allow VBP cord-104239-xxlcdbqi 431 31 us -PRON- PRP cord-104239-xxlcdbqi 431 32 to to TO cord-104239-xxlcdbqi 431 33 directly directly RB cord-104239-xxlcdbqi 431 34 assess assess VB cord-104239-xxlcdbqi 431 35 the the DT cord-104239-xxlcdbqi 431 36 concentration concentration NN cord-104239-xxlcdbqi 431 37 of of IN cord-104239-xxlcdbqi 431 38 VSV VSV NNP cord-104239-xxlcdbqi 431 39 - - HYPH cord-104239-xxlcdbqi 431 40 G G NNP cord-104239-xxlcdbqi 431 41 in in IN cord-104239-xxlcdbqi 431 42 ER er NN cord-104239-xxlcdbqi 431 43 - - HYPH cord-104239-xxlcdbqi 431 44 derived derive VBN cord-104239-xxlcdbqi 431 45 vesicles vesicle NNS cord-104239-xxlcdbqi 431 46 . . . cord-104239-xxlcdbqi 432 1 We -PRON- PRP cord-104239-xxlcdbqi 432 2 found find VBD cord-104239-xxlcdbqi 432 3 , , , cord-104239-xxlcdbqi 432 4 on on IN cord-104239-xxlcdbqi 432 5 average average JJ cord-104239-xxlcdbqi 432 6 , , , cord-104239-xxlcdbqi 432 7 a a DT cord-104239-xxlcdbqi 432 8 value value NN cord-104239-xxlcdbqi 432 9 of of IN cord-104239-xxlcdbqi 432 10 ~800 ~800 JJ cord-104239-xxlcdbqi 432 11 gold gold NN cord-104239-xxlcdbqi 432 12 particles particle NNS cord-104239-xxlcdbqi 432 13 per per IN cord-104239-xxlcdbqi 432 14 txm txm NNP cord-104239-xxlcdbqi 432 15 2 2 CD cord-104239-xxlcdbqi 432 16 in in IN cord-104239-xxlcdbqi 432 17 planar planar JJ cord-104239-xxlcdbqi 432 18 projection projection NN cord-104239-xxlcdbqi 432 19 of of IN cord-104239-xxlcdbqi 432 20 clusters cluster NNS cord-104239-xxlcdbqi 432 21 accumulated accumulate VBN cord-104239-xxlcdbqi 432 22 in in IN cord-104239-xxlcdbqi 432 23 either either CC cord-104239-xxlcdbqi 432 24 the the DT cord-104239-xxlcdbqi 432 25 absence absence NN cord-104239-xxlcdbqi 432 26 or or CC cord-104239-xxlcdbqi 432 27 presence presence NN cord-104239-xxlcdbqi 432 28 of of IN cord-104239-xxlcdbqi 432 29 GTP GTP NNP cord-104239-xxlcdbqi 432 30 ~ ~ NFP cord-104239-xxlcdbqi 432 31 S S NNP cord-104239-xxlcdbqi 432 32 , , , cord-104239-xxlcdbqi 432 33 or or CC cord-104239-xxlcdbqi 432 34 in in IN cord-104239-xxlcdbqi 432 35 the the DT cord-104239-xxlcdbqi 432 36 presence presence NN cord-104239-xxlcdbqi 432 37 of of IN cord-104239-xxlcdbqi 432 38 the the DT cord-104239-xxlcdbqi 432 39 activated activate VBN cord-104239-xxlcdbqi 432 40 Sarl Sarl NNP cord-104239-xxlcdbqi 432 41 - - HYPH cord-104239-xxlcdbqi 432 42 GTP GTP NNP cord-104239-xxlcdbqi 432 43 restricted restrict VBN cord-104239-xxlcdbqi 432 44 mutant mutant NN cord-104239-xxlcdbqi 432 45 . . . cord-104239-xxlcdbqi 433 1 The the DT cord-104239-xxlcdbqi 433 2 inclusion inclusion NN cord-104239-xxlcdbqi 433 3 of of IN cord-104239-xxlcdbqi 433 4 the the DT cord-104239-xxlcdbqi 433 5 inhibitors inhibitor NNS cord-104239-xxlcdbqi 433 6 prevents prevent VBZ cord-104239-xxlcdbqi 433 7 further further JJ cord-104239-xxlcdbqi 433 8 rounds round NNS cord-104239-xxlcdbqi 433 9 of of IN cord-104239-xxlcdbqi 433 10 vesicle vesicle NN cord-104239-xxlcdbqi 433 11 budding budding NN cord-104239-xxlcdbqi 433 12 , , , cord-104239-xxlcdbqi 433 13 ensuring ensure VBG cord-104239-xxlcdbqi 433 14 that that IN cord-104239-xxlcdbqi 433 15 we -PRON- PRP cord-104239-xxlcdbqi 433 16 are be VBP cord-104239-xxlcdbqi 433 17 examining examine VBG cord-104239-xxlcdbqi 433 18 concentration concentration NN cord-104239-xxlcdbqi 433 19 associated associate VBN cord-104239-xxlcdbqi 433 20 with with IN cord-104239-xxlcdbqi 433 21 export export NN cord-104239-xxlcdbqi 433 22 from from IN cord-104239-xxlcdbqi 433 23 the the DT cord-104239-xxlcdbqi 433 24 ER ER NNP cord-104239-xxlcdbqi 433 25 . . . cord-104239-xxlcdbqi 434 1 A a DT cord-104239-xxlcdbqi 434 2 planar planar JJ cord-104239-xxlcdbqi 434 3 projection projection NN cord-104239-xxlcdbqi 434 4 , , , cord-104239-xxlcdbqi 434 5 however however RB cord-104239-xxlcdbqi 434 6 , , , cord-104239-xxlcdbqi 434 7 is be VBZ cord-104239-xxlcdbqi 434 8 not not RB cord-104239-xxlcdbqi 434 9 a a DT cord-104239-xxlcdbqi 434 10 good good JJ cord-104239-xxlcdbqi 434 11 approximation approximation NN cord-104239-xxlcdbqi 434 12 of of IN cord-104239-xxlcdbqi 434 13 the the DT cord-104239-xxlcdbqi 434 14 total total JJ cord-104239-xxlcdbqi 434 15 surface surface NN cord-104239-xxlcdbqi 434 16 area area NN cord-104239-xxlcdbqi 434 17 available available JJ cord-104239-xxlcdbqi 434 18 on on IN cord-104239-xxlcdbqi 434 19 clusters cluster NNS cord-104239-xxlcdbqi 434 20 for for IN cord-104239-xxlcdbqi 434 21 antibody antibody NN cord-104239-xxlcdbqi 434 22 binding binding NN cord-104239-xxlcdbqi 434 23 . . . cord-104239-xxlcdbqi 435 1 Since since IN cord-104239-xxlcdbqi 435 2 only only RB cord-104239-xxlcdbqi 435 3 the the DT cord-104239-xxlcdbqi 435 4 external external JJ cord-104239-xxlcdbqi 435 5 surface surface NN cord-104239-xxlcdbqi 435 6 of of IN cord-104239-xxlcdbqi 435 7 vesicles vesicle NNS cord-104239-xxlcdbqi 435 8 found find VBN cord-104239-xxlcdbqi 435 9 on on IN cord-104239-xxlcdbqi 435 10 the the DT cord-104239-xxlcdbqi 435 11 perimeter perimeter NN cord-104239-xxlcdbqi 435 12 of of IN cord-104239-xxlcdbqi 435 13 clusters cluster NNS cord-104239-xxlcdbqi 435 14 is be VBZ cord-104239-xxlcdbqi 435 15 available available JJ cord-104239-xxlcdbqi 435 16 for for IN cord-104239-xxlcdbqi 435 17 antibody antibody NN cord-104239-xxlcdbqi 435 18 binding bind VBG cord-104239-xxlcdbqi 435 19 Pind Pind NNP cord-104239-xxlcdbqi 435 20 et et FW cord-104239-xxlcdbqi 435 21 al al NNP cord-104239-xxlcdbqi 435 22 . . NNP cord-104239-xxlcdbqi 435 23 , , , cord-104239-xxlcdbqi 435 24 1994a 1994a CD cord-104239-xxlcdbqi 435 25 ) ) -RRB- cord-104239-xxlcdbqi 436 1 ( ( -LRB- cord-104239-xxlcdbqi 436 2 see see VB cord-104239-xxlcdbqi 436 3 Fig Fig NNP cord-104239-xxlcdbqi 436 4 . . . cord-104239-xxlcdbqi 437 1 11 11 CD cord-104239-xxlcdbqi 437 2 D D NNP cord-104239-xxlcdbqi 437 3 ) ) -RRB- cord-104239-xxlcdbqi 437 4 , , , cord-104239-xxlcdbqi 437 5 a a DT cord-104239-xxlcdbqi 437 6 more more RBR cord-104239-xxlcdbqi 437 7 reasonable reasonable JJ cord-104239-xxlcdbqi 437 8 estimate estimate NN cord-104239-xxlcdbqi 437 9 of of IN cord-104239-xxlcdbqi 437 10 VSV VSV NNP cord-104239-xxlcdbqi 437 11 - - HYPH cord-104239-xxlcdbqi 437 12 G g NN cord-104239-xxlcdbqi 437 13 density density NN cord-104239-xxlcdbqi 437 14 can can MD cord-104239-xxlcdbqi 437 15 be be VB cord-104239-xxlcdbqi 437 16 determined determine VBN cord-104239-xxlcdbqi 437 17 if if IN cord-104239-xxlcdbqi 437 18 we -PRON- PRP cord-104239-xxlcdbqi 437 19 assume assume VBP cord-104239-xxlcdbqi 437 20 that that IN cord-104239-xxlcdbqi 437 21 the the DT cord-104239-xxlcdbqi 437 22 antibody antibody NN cord-104239-xxlcdbqi 437 23 has have VBZ cord-104239-xxlcdbqi 437 24 access access NN cord-104239-xxlcdbqi 437 25 to to IN cord-104239-xxlcdbqi 437 26 the the DT cord-104239-xxlcdbqi 437 27 outer outer JJ cord-104239-xxlcdbqi 437 28 - - HYPH cord-104239-xxlcdbqi 437 29 half half NN cord-104239-xxlcdbqi 437 30 of of IN cord-104239-xxlcdbqi 437 31 a a DT cord-104239-xxlcdbqi 437 32 shell shell NN cord-104239-xxlcdbqi 437 33 of of IN cord-104239-xxlcdbqi 437 34 80-nm 80-nm CD cord-104239-xxlcdbqi 437 35 vesicles vesicle NNS cord-104239-xxlcdbqi 437 36 that that WDT cord-104239-xxlcdbqi 437 37 occupy occupy VBP cord-104239-xxlcdbqi 437 38 the the DT cord-104239-xxlcdbqi 437 39 perimeter perimeter NN cord-104239-xxlcdbqi 437 40 of of IN cord-104239-xxlcdbqi 437 41 a a DT cord-104239-xxlcdbqi 437 42 0.4-~m 0.4-~m CD cord-104239-xxlcdbqi 437 43 sphere sphere NN cord-104239-xxlcdbqi 437 44 ( ( -LRB- cord-104239-xxlcdbqi 437 45 the the DT cord-104239-xxlcdbqi 437 46 size size NN cord-104239-xxlcdbqi 437 47 of of IN cord-104239-xxlcdbqi 437 48 a a DT cord-104239-xxlcdbqi 437 49 typical typical JJ cord-104239-xxlcdbqi 437 50 VTC vtc NN cord-104239-xxlcdbqi 437 51 ) ) -RRB- cord-104239-xxlcdbqi 437 52 . . . cord-104239-xxlcdbqi 438 1 Compared compare VBN cord-104239-xxlcdbqi 438 2 with with IN cord-104239-xxlcdbqi 438 3 the the DT cord-104239-xxlcdbqi 438 4 surface surface NN cord-104239-xxlcdbqi 438 5 area area NN cord-104239-xxlcdbqi 438 6 of of IN cord-104239-xxlcdbqi 438 7 the the DT cord-104239-xxlcdbqi 438 8 planar planar JJ cord-104239-xxlcdbqi 438 9 projection projection NN cord-104239-xxlcdbqi 438 10 ( ( -LRB- cord-104239-xxlcdbqi 438 11 ~0.13 ~0.13 NFP cord-104239-xxlcdbqi 438 12 ~m2 ~m2 NN cord-104239-xxlcdbqi 438 13 ) ) -RRB- cord-104239-xxlcdbqi 438 14 , , , cord-104239-xxlcdbqi 438 15 the the DT cord-104239-xxlcdbqi 438 16 surface surface NN cord-104239-xxlcdbqi 438 17 area area NN cord-104239-xxlcdbqi 438 18 of of IN cord-104239-xxlcdbqi 438 19 such such PDT cord-104239-xxlcdbqi 438 20 a a DT cord-104239-xxlcdbqi 438 21 population population NN cord-104239-xxlcdbqi 438 22 of of IN cord-104239-xxlcdbqi 438 23 vesicles vesicle NNS cord-104239-xxlcdbqi 438 24 corresponds correspond VBZ cord-104239-xxlcdbqi 438 25 to to IN cord-104239-xxlcdbqi 438 26 a a DT cord-104239-xxlcdbqi 438 27 value value NN cord-104239-xxlcdbqi 438 28 of of IN cord-104239-xxlcdbqi 438 29 ~0.7 ~0.7 CD cord-104239-xxlcdbqi 438 30 txm txm NNP cord-104239-xxlcdbqi 438 31 2 2 CD cord-104239-xxlcdbqi 438 32 . . . cord-104239-xxlcdbqi 439 1 This this DT cord-104239-xxlcdbqi 439 2 is be VBZ cord-104239-xxlcdbqi 439 3 an an DT cord-104239-xxlcdbqi 439 4 increase increase NN cord-104239-xxlcdbqi 439 5 of of IN cord-104239-xxlcdbqi 439 6 approximately approximately RB cord-104239-xxlcdbqi 439 7 fivefold fivefold JJ cord-104239-xxlcdbqi 439 8 over over IN cord-104239-xxlcdbqi 439 9 the the DT cord-104239-xxlcdbqi 439 10 planar planar JJ cord-104239-xxlcdbqi 439 11 projection projection NN cord-104239-xxlcdbqi 439 12 . . . cord-104239-xxlcdbqi 440 1 Therefore therefore RB cord-104239-xxlcdbqi 440 2 the the DT cord-104239-xxlcdbqi 440 3 actual actual JJ cord-104239-xxlcdbqi 440 4 surface surface NN cord-104239-xxlcdbqi 440 5 density density NN cord-104239-xxlcdbqi 440 6 of of IN cord-104239-xxlcdbqi 440 7 VSV VSV NNP cord-104239-xxlcdbqi 440 8 - - HYPH cord-104239-xxlcdbqi 440 9 G G NNP cord-104239-xxlcdbqi 440 10 in in IN cord-104239-xxlcdbqi 440 11 clusters cluster NNS cord-104239-xxlcdbqi 440 12 observed observe VBN cord-104239-xxlcdbqi 440 13 in in IN cord-104239-xxlcdbqi 440 14 replicas replica NNS cord-104239-xxlcdbqi 440 15 is be VBZ cord-104239-xxlcdbqi 440 16 800 800 CD cord-104239-xxlcdbqi 440 17 gold gold NN cord-104239-xxlcdbqi 440 18 particles particle NNS cord-104239-xxlcdbqi 440 19 per per IN cord-104239-xxlcdbqi 440 20 i i NN cord-104239-xxlcdbqi 440 21 ~ ~ CD cord-104239-xxlcdbqi 440 22 m m NN cord-104239-xxlcdbqi 440 23 2 2 CD cord-104239-xxlcdbqi 440 24 divided divide VBN cord-104239-xxlcdbqi 440 25 by by IN cord-104239-xxlcdbqi 440 26 5 5 CD cord-104239-xxlcdbqi 440 27 or or CC cord-104239-xxlcdbqi 440 28 160 160 CD cord-104239-xxlcdbqi 440 29 gold gold NN cord-104239-xxlcdbqi 440 30 particles particle NNS cord-104239-xxlcdbqi 440 31 per per IN cord-104239-xxlcdbqi 440 32 ixm ixm NN cord-104239-xxlcdbqi 440 33 2 2 CD cord-104239-xxlcdbqi 440 34 . . . cord-104239-xxlcdbqi 441 1 This this DT cord-104239-xxlcdbqi 441 2 value value NN cord-104239-xxlcdbqi 441 3 , , , cord-104239-xxlcdbqi 441 4 when when WRB cord-104239-xxlcdbqi 441 5 compared compare VBN cord-104239-xxlcdbqi 441 6 to to IN cord-104239-xxlcdbqi 441 7 the the DT cord-104239-xxlcdbqi 441 8 average average JJ cord-104239-xxlcdbqi 441 9 surface surface NN cord-104239-xxlcdbqi 441 10 density density NN cord-104239-xxlcdbqi 441 11 of of IN cord-104239-xxlcdbqi 441 12 VSV VSV NNP cord-104239-xxlcdbqi 441 13 - - HYPH cord-104239-xxlcdbqi 441 14 G G NNP cord-104239-xxlcdbqi 441 15 before before IN cord-104239-xxlcdbqi 441 16 incubation incubation NN cord-104239-xxlcdbqi 441 17 in in IN cord-104239-xxlcdbqi 441 18 vitro vitro FW cord-104239-xxlcdbqi 441 19 ( ( -LRB- cord-104239-xxlcdbqi 441 20 32 32 CD cord-104239-xxlcdbqi 441 21 gold gold NN cord-104239-xxlcdbqi 441 22 particles particle NNS cord-104239-xxlcdbqi 441 23 per per IN cord-104239-xxlcdbqi 441 24 ~m2 ~m2 NN cord-104239-xxlcdbqi 441 25 ) ) -RRB- cord-104239-xxlcdbqi 441 26 , , , cord-104239-xxlcdbqi 441 27 sug- sug- NN cord-104239-xxlcdbqi 441 28 Figure figure NN cord-104239-xxlcdbqi 441 29 11 11 CD cord-104239-xxlcdbqi 441 30 . . . cord-104239-xxlcdbqi 442 1 Diagram diagram NN cord-104239-xxlcdbqi 442 2 summarizing summarize VBG cord-104239-xxlcdbqi 442 3 the the DT cord-104239-xxlcdbqi 442 4 three three CD cord-104239-xxlcdbqi 442 5 tiers tier NNS cord-104239-xxlcdbqi 442 6 of of IN cord-104239-xxlcdbqi 442 7 organization organization NN cord-104239-xxlcdbqi 442 8 of of IN cord-104239-xxlcdbqi 442 9 ER ER NNP cord-104239-xxlcdbqi 442 10 export export NN cord-104239-xxlcdbqi 442 11 complexes complex NNS cord-104239-xxlcdbqi 442 12 in in IN cord-104239-xxlcdbqi 442 13 vivo vivo NNP cord-104239-xxlcdbqi 442 14 ( ( -LRB- cord-104239-xxlcdbqi 442 15 A a NN cord-104239-xxlcdbqi 442 16 ) ) -RRB- cord-104239-xxlcdbqi 442 17 and and CC cord-104239-xxlcdbqi 442 18 in in IN cord-104239-xxlcdbqi 442 19 vitro vitro FW cord-104239-xxlcdbqi 442 20 ( ( -LRB- cord-104239-xxlcdbqi 442 21 B b NN cord-104239-xxlcdbqi 442 22 ) ) -RRB- cord-104239-xxlcdbqi 442 23 . . . cord-104239-xxlcdbqi 443 1 ( ( -LRB- cord-104239-xxlcdbqi 443 2 A a NN cord-104239-xxlcdbqi 443 3 ) ) -RRB- cord-104239-xxlcdbqi 444 1 An an DT cord-104239-xxlcdbqi 444 2 individual individual JJ cord-104239-xxlcdbqi 444 3 ER ER NNP cord-104239-xxlcdbqi 444 4 cisterna cisterna NNP cord-104239-xxlcdbqi 444 5 contains contain VBZ cord-104239-xxlcdbqi 444 6 a a DT cord-104239-xxlcdbqi 444 7 collection collection NN cord-104239-xxlcdbqi 444 8 of of IN cord-104239-xxlcdbqi 444 9 closely closely RB cord-104239-xxlcdbqi 444 10 opposed oppose VBN cord-104239-xxlcdbqi 444 11 buds bud NNS cord-104239-xxlcdbqi 444 12 that that WDT cord-104239-xxlcdbqi 444 13 define define VBP cord-104239-xxlcdbqi 444 14 a a DT cord-104239-xxlcdbqi 444 15 local local JJ cord-104239-xxlcdbqi 444 16 transitional transitional JJ cord-104239-xxlcdbqi 444 17 region region NN cord-104239-xxlcdbqi 444 18 ( ( -LRB- cord-104239-xxlcdbqi 444 19 light light JJ cord-104239-xxlcdbqi 444 20 zone zone NN cord-104239-xxlcdbqi 444 21 with with IN cord-104239-xxlcdbqi 444 22 buds bud NNS cord-104239-xxlcdbqi 444 23 on on IN cord-104239-xxlcdbqi 444 24 the the DT cord-104239-xxlcdbqi 444 25 ER er NN cord-104239-xxlcdbqi 444 26 ) ) -RRB- cord-104239-xxlcdbqi 444 27 . . . cord-104239-xxlcdbqi 445 1 This this DT cord-104239-xxlcdbqi 445 2 specialized specialized JJ cord-104239-xxlcdbqi 445 3 region region NN cord-104239-xxlcdbqi 445 4 is be VBZ cord-104239-xxlcdbqi 445 5 dominated dominate VBN cord-104239-xxlcdbqi 445 6 by by IN cord-104239-xxlcdbqi 445 7 the the DT cord-104239-xxlcdbqi 445 8 presence presence NN cord-104239-xxlcdbqi 445 9 of of IN cord-104239-xxlcdbqi 445 10 COPII COPII NNP cord-104239-xxlcdbqi 445 11 coats coat NNS cord-104239-xxlcdbqi 445 12 and and CC cord-104239-xxlcdbqi 445 13 is be VBZ cord-104239-xxlcdbqi 445 14 referred refer VBN cord-104239-xxlcdbqi 445 15 to to IN cord-104239-xxlcdbqi 445 16 as as IN cord-104239-xxlcdbqi 445 17 tier tier NN cord-104239-xxlcdbqi 445 18 1 1 CD cord-104239-xxlcdbqi 445 19 ( ( -LRB- cord-104239-xxlcdbqi 445 20 box box NN cord-104239-xxlcdbqi 445 21 outlined outline VBN cord-104239-xxlcdbqi 445 22 with with IN cord-104239-xxlcdbqi 445 23 dotted dotted JJ cord-104239-xxlcdbqi 445 24 line line NN cord-104239-xxlcdbqi 445 25 ) ) -RRB- cord-104239-xxlcdbqi 445 26 . . . cord-104239-xxlcdbqi 446 1 Tier Tier NNP cord-104239-xxlcdbqi 446 2 II II NNP cord-104239-xxlcdbqi 446 3 ( ( -LRB- cord-104239-xxlcdbqi 446 4 cylindrical cylindrical JJ cord-104239-xxlcdbqi 446 5 region region NN cord-104239-xxlcdbqi 446 6 outlined outline VBN cord-104239-xxlcdbqi 446 7 by by IN cord-104239-xxlcdbqi 446 8 dashed dash VBN cord-104239-xxlcdbqi 446 9 lines line NNS cord-104239-xxlcdbqi 446 10 ) ) -RRB- cord-104239-xxlcdbqi 446 11 includes include VBZ cord-104239-xxlcdbqi 446 12 buds bud NNS cord-104239-xxlcdbqi 446 13 on on IN cord-104239-xxlcdbqi 446 14 distantly distantly RB cord-104239-xxlcdbqi 446 15 connected connect VBN cord-104239-xxlcdbqi 446 16 ER ER NNP cord-104239-xxlcdbqi 446 17 strands strand NNS cord-104239-xxlcdbqi 446 18 that that WDT cord-104239-xxlcdbqi 446 19 face face VBP cord-104239-xxlcdbqi 446 20 a a DT cord-104239-xxlcdbqi 446 21 central central JJ cord-104239-xxlcdbqi 446 22 VTC vtc NN cord-104239-xxlcdbqi 446 23 consisting consist VBG cord-104239-xxlcdbqi 446 24 of of IN cord-104239-xxlcdbqi 446 25 a a DT cord-104239-xxlcdbqi 446 26 collection collection NN cord-104239-xxlcdbqi 446 27 of of IN cord-104239-xxlcdbqi 446 28 distinct distinct JJ cord-104239-xxlcdbqi 446 29 vesicular vesicular JJ cord-104239-xxlcdbqi 446 30 - - HYPH cord-104239-xxlcdbqi 446 31 tubular tubular JJ cord-104239-xxlcdbqi 446 32 elements element NNS cord-104239-xxlcdbqi 446 33 that that WDT cord-104239-xxlcdbqi 446 34 have have VBP cord-104239-xxlcdbqi 446 35 COPI COPI NNP cord-104239-xxlcdbqi 446 36 coats coat NNS cord-104239-xxlcdbqi 446 37 . . . cord-104239-xxlcdbqi 447 1 Tier Tier NNP cord-104239-xxlcdbqi 447 2 III III NNP cord-104239-xxlcdbqi 447 3 includes include VBZ cord-104239-xxlcdbqi 447 4 the the DT cord-104239-xxlcdbqi 447 5 entire entire JJ cord-104239-xxlcdbqi 447 6 export export NN cord-104239-xxlcdbqi 447 7 complex complex NN cord-104239-xxlcdbqi 447 8 and and CC cord-104239-xxlcdbqi 447 9 is be VBZ cord-104239-xxlcdbqi 447 10 outlined outline VBN cord-104239-xxlcdbqi 447 11 by by IN cord-104239-xxlcdbqi 447 12 the the DT cord-104239-xxlcdbqi 447 13 box box NN cord-104239-xxlcdbqi 447 14 with with IN cord-104239-xxlcdbqi 447 15 the the DT cord-104239-xxlcdbqi 447 16 solid solid JJ cord-104239-xxlcdbqi 447 17 line line NN cord-104239-xxlcdbqi 447 18 that that WDT cord-104239-xxlcdbqi 447 19 encompasses encompass VBZ cord-104239-xxlcdbqi 447 20 both both CC cord-104239-xxlcdbqi 447 21 ER ER NNP cord-104239-xxlcdbqi 447 22 buds bud NNS cord-104239-xxlcdbqi 447 23 and and CC cord-104239-xxlcdbqi 447 24 a a DT cord-104239-xxlcdbqi 447 25 central central JJ cord-104239-xxlcdbqi 447 26 VTC vtc NN cord-104239-xxlcdbqi 447 27 . . . cord-104239-xxlcdbqi 448 1 The the DT cord-104239-xxlcdbqi 448 2 possible possible JJ cord-104239-xxlcdbqi 448 3 elevated elevated JJ cord-104239-xxlcdbqi 448 4 concentration concentration NN cord-104239-xxlcdbqi 448 5 of of IN cord-104239-xxlcdbqi 448 6 COPII COPII NNP cord-104239-xxlcdbqi 448 7 and and CC cord-104239-xxlcdbqi 448 8 COPI COPI NNP cord-104239-xxlcdbqi 448 9 coat coat NN cord-104239-xxlcdbqi 448 10 components component NNS cord-104239-xxlcdbqi 448 11 within within IN cord-104239-xxlcdbqi 448 12 the the DT cord-104239-xxlcdbqi 448 13 local local JJ cord-104239-xxlcdbqi 448 14 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 448 15 of of IN cord-104239-xxlcdbqi 448 16 export export NN cord-104239-xxlcdbqi 448 17 complexes complex NNS cord-104239-xxlcdbqi 448 18 is be VBZ cord-104239-xxlcdbqi 448 19 depicted depict VBN cord-104239-xxlcdbqi 448 20 by by IN cord-104239-xxlcdbqi 448 21 small small JJ cord-104239-xxlcdbqi 448 22 dots dot NNS cord-104239-xxlcdbqi 448 23 and and CC cord-104239-xxlcdbqi 448 24 lines line NNS cord-104239-xxlcdbqi 448 25 . . . cord-104239-xxlcdbqi 449 1 ( ( -LRB- cord-104239-xxlcdbqi 449 2 B b NN cord-104239-xxlcdbqi 449 3 ) ) -RRB- cord-104239-xxlcdbqi 450 1 In in IN cord-104239-xxlcdbqi 450 2 semi semi JJ cord-104239-xxlcdbqi 450 3 - - JJ cord-104239-xxlcdbqi 450 4 intact intact JJ cord-104239-xxlcdbqi 450 5 cells cell NNS cord-104239-xxlcdbqi 450 6 , , , cord-104239-xxlcdbqi 450 7 there there EX cord-104239-xxlcdbqi 450 8 appears appear VBZ cord-104239-xxlcdbqi 450 9 to to TO cord-104239-xxlcdbqi 450 10 be be VB cord-104239-xxlcdbqi 450 11 a a DT cord-104239-xxlcdbqi 450 12 more more RBR cord-104239-xxlcdbqi 450 13 limited limited JJ cord-104239-xxlcdbqi 450 14 number number NN cord-104239-xxlcdbqi 450 15 of of IN cord-104239-xxlcdbqi 450 16 ER ER NNP cord-104239-xxlcdbqi 450 17 buds bud NNS cord-104239-xxlcdbqi 450 18 associated associate VBN cord-104239-xxlcdbqi 450 19 with with IN cord-104239-xxlcdbqi 450 20 the the DT cord-104239-xxlcdbqi 450 21 local local JJ cord-104239-xxlcdbqi 450 22 transitional transitional JJ cord-104239-xxlcdbqi 450 23 region region NN cord-104239-xxlcdbqi 450 24 defined define VBN cord-104239-xxlcdbqi 450 25 by by IN cord-104239-xxlcdbqi 450 26 tier tier NN cord-104239-xxlcdbqi 450 27 I I NNP cord-104239-xxlcdbqi 450 28 ( ( -LRB- cord-104239-xxlcdbqi 450 29 box box NN cord-104239-xxlcdbqi 450 30 outlined outline VBN cord-104239-xxlcdbqi 450 31 with with IN cord-104239-xxlcdbqi 450 32 dotted dotted JJ cord-104239-xxlcdbqi 450 33 lines line NNS cord-104239-xxlcdbqi 450 34 ) ) -RRB- cord-104239-xxlcdbqi 450 35 . . . cord-104239-xxlcdbqi 451 1 The the DT cord-104239-xxlcdbqi 451 2 tier tier NN cord-104239-xxlcdbqi 451 3 iI ii CD cord-104239-xxlcdbqi 451 4 level level NN cord-104239-xxlcdbqi 451 5 of of IN cord-104239-xxlcdbqi 451 6 organization organization NN cord-104239-xxlcdbqi 451 7 is be VBZ cord-104239-xxlcdbqi 451 8 completely completely RB cord-104239-xxlcdbqi 451 9 missing miss VBG cord-104239-xxlcdbqi 451 10 in in IN cord-104239-xxlcdbqi 451 11 semi semi JJ cord-104239-xxlcdbqi 451 12 - - JJ cord-104239-xxlcdbqi 451 13 intact intact JJ cord-104239-xxlcdbqi 451 14 cells cell NNS cord-104239-xxlcdbqi 451 15 , , , cord-104239-xxlcdbqi 451 16 as as IN cord-104239-xxlcdbqi 451 17 the the DT cord-104239-xxlcdbqi 451 18 association association NN cord-104239-xxlcdbqi 451 19 of of IN cord-104239-xxlcdbqi 451 20 distantly distantly RB cord-104239-xxlcdbqi 451 21 connected connect VBN cord-104239-xxlcdbqi 451 22 ER ER NNP cord-104239-xxlcdbqi 451 23 strands strand NNS cord-104239-xxlcdbqi 451 24 appears appear VBZ cord-104239-xxlcdbqi 451 25 to to TO cord-104239-xxlcdbqi 451 26 be be VB cord-104239-xxlcdbqi 451 27 lost lose VBN cord-104239-xxlcdbqi 451 28 during during IN cord-104239-xxlcdbqi 451 29 cell cell NN cord-104239-xxlcdbqi 451 30 permeabilization permeabilization NN cord-104239-xxlcdbqi 451 31 . . . cord-104239-xxlcdbqi 452 1 However however RB cord-104239-xxlcdbqi 452 2 , , , cord-104239-xxlcdbqi 452 3 tier tier NN cord-104239-xxlcdbqi 452 4 III III NNP cord-104239-xxlcdbqi 452 5 ( ( -LRB- cord-104239-xxlcdbqi 452 6 box box NN cord-104239-xxlcdbqi 452 7 outlined outline VBN cord-104239-xxlcdbqi 452 8 with with IN cord-104239-xxlcdbqi 452 9 solid solid JJ cord-104239-xxlcdbqi 452 10 line line NN cord-104239-xxlcdbqi 452 11 ) ) -RRB- cord-104239-xxlcdbqi 452 12 is be VBZ cord-104239-xxlcdbqi 452 13 maintained maintain VBN cord-104239-xxlcdbqi 452 14 , , , cord-104239-xxlcdbqi 452 15 highlighting highlight VBG cord-104239-xxlcdbqi 452 16 the the DT cord-104239-xxlcdbqi 452 17 juxtaposition juxtaposition NN cord-104239-xxlcdbqi 452 18 of of IN cord-104239-xxlcdbqi 452 19 VTCs vtc NNS cord-104239-xxlcdbqi 452 20 to to IN cord-104239-xxlcdbqi 452 21 buds bud NNS cord-104239-xxlcdbqi 452 22 on on IN cord-104239-xxlcdbqi 452 23 one one CD cord-104239-xxlcdbqi 452 24 ER ER NNP cord-104239-xxlcdbqi 452 25 strand strand NN cord-104239-xxlcdbqi 452 26 . . . cord-104239-xxlcdbqi 453 1 gests gests NNP cord-104239-xxlcdbqi 453 2 that that WDT cord-104239-xxlcdbqi 454 1 VSV VSV NNP cord-104239-xxlcdbqi 454 2 - - HYPH cord-104239-xxlcdbqi 454 3 G G NNP cord-104239-xxlcdbqi 454 4 is be VBZ cord-104239-xxlcdbqi 454 5 concentrated concentrate VBN cord-104239-xxlcdbqi 454 6 five five CD cord-104239-xxlcdbqi 454 7 - - HYPH cord-104239-xxlcdbqi 454 8 to to TO cord-104239-xxlcdbqi 454 9 sixfold sixfold VB cord-104239-xxlcdbqi 454 10 during during IN cord-104239-xxlcdbqi 454 11 budding bud VBG cord-104239-xxlcdbqi 454 12 from from IN cord-104239-xxlcdbqi 454 13 the the DT cord-104239-xxlcdbqi 454 14 ER ER NNP cord-104239-xxlcdbqi 454 15 . . . cord-104239-xxlcdbqi 455 1 The the DT cord-104239-xxlcdbqi 455 2 fold fold JJ cord-104239-xxlcdbqi 455 3 - - HYPH cord-104239-xxlcdbqi 455 4 concentration concentration NN cord-104239-xxlcdbqi 455 5 detected detect VBN cord-104239-xxlcdbqi 455 6 here here RB cord-104239-xxlcdbqi 455 7 is be VBZ cord-104239-xxlcdbqi 455 8 very very RB cord-104239-xxlcdbqi 455 9 consistent consistent JJ cord-104239-xxlcdbqi 455 10 with with IN cord-104239-xxlcdbqi 455 11 that that DT cord-104239-xxlcdbqi 455 12 observed observe VBD cord-104239-xxlcdbqi 455 13 previously previously RB cord-104239-xxlcdbqi 455 14 using use VBG cord-104239-xxlcdbqi 455 15 serial serial JJ cord-104239-xxlcdbqi 455 16 thin thin JJ cord-104239-xxlcdbqi 455 17 - - HYPH cord-104239-xxlcdbqi 455 18 sections section NNS cord-104239-xxlcdbqi 455 19 Pind Pind NNP cord-104239-xxlcdbqi 455 20 et et NNP cord-104239-xxlcdbqi 455 21 al al NNP cord-104239-xxlcdbqi 455 22 . . NNP cord-104239-xxlcdbqi 455 23 , , , cord-104239-xxlcdbqi 455 24 1994a 1994a CD cord-104239-xxlcdbqi 455 25 ) ) -RRB- cord-104239-xxlcdbqi 455 26 or or CC cord-104239-xxlcdbqi 455 27 in in IN cord-104239-xxlcdbqi 455 28 the the DT cord-104239-xxlcdbqi 455 29 present present JJ cord-104239-xxlcdbqi 455 30 studies study NNS cord-104239-xxlcdbqi 455 31 in in IN cord-104239-xxlcdbqi 455 32 the the DT cord-104239-xxlcdbqi 455 33 presence presence NN cord-104239-xxlcdbqi 455 34 of of IN cord-104239-xxlcdbqi 455 35 the the DT cord-104239-xxlcdbqi 455 36 Sarl Sarl NNP cord-104239-xxlcdbqi 455 37 - - HYPH cord-104239-xxlcdbqi 455 38 GTP GTP NNP cord-104239-xxlcdbqi 455 39 restricted restrict VBN cord-104239-xxlcdbqi 455 40 mutant mutant NN cord-104239-xxlcdbqi 455 41 ( ( -LRB- cord-104239-xxlcdbqi 455 42 Table Table NNP cord-104239-xxlcdbqi 455 43 III III NNP cord-104239-xxlcdbqi 455 44 ) ) -RRB- cord-104239-xxlcdbqi 455 45 . . . cord-104239-xxlcdbqi 456 1 These these DT cord-104239-xxlcdbqi 456 2 results result NNS cord-104239-xxlcdbqi 456 3 confirm confirm VBP cord-104239-xxlcdbqi 456 4 the the DT cord-104239-xxlcdbqi 456 5 validity validity NN cord-104239-xxlcdbqi 456 6 of of IN cord-104239-xxlcdbqi 456 7 our -PRON- PRP$ cord-104239-xxlcdbqi 456 8 previous previous JJ cord-104239-xxlcdbqi 456 9 technical technical JJ cord-104239-xxlcdbqi 456 10 advancements advancement NNS cord-104239-xxlcdbqi 456 11 Balch Balch NNP cord-104239-xxlcdbqi 456 12 and and CC cord-104239-xxlcdbqi 456 13 Farquhar Farquhar NNP cord-104239-xxlcdbqi 456 14 , , , cord-104239-xxlcdbqi 456 15 1995 1995 CD cord-104239-xxlcdbqi 456 16 ) ) -RRB- cord-104239-xxlcdbqi 456 17 and and CC cord-104239-xxlcdbqi 456 18 now now RB cord-104239-xxlcdbqi 456 19 firmly firmly RB cord-104239-xxlcdbqi 456 20 establish establish VBP cord-104239-xxlcdbqi 456 21 that that DT cord-104239-xxlcdbqi 456 22 cargo cargo NN cord-104239-xxlcdbqi 456 23 is be VBZ cord-104239-xxlcdbqi 456 24 concentrated concentrate VBN cord-104239-xxlcdbqi 456 25 during during IN cord-104239-xxlcdbqi 456 26 ER ER NNP cord-104239-xxlcdbqi 456 27 export export NN cord-104239-xxlcdbqi 456 28 . . . cord-104239-xxlcdbqi 457 1 Sequential sequential JJ cord-104239-xxlcdbqi 457 2 coupling coupling NN cord-104239-xxlcdbqi 457 3 between between IN cord-104239-xxlcdbqi 457 4 COPII COPII NNP cord-104239-xxlcdbqi 457 5 and and CC cord-104239-xxlcdbqi 457 6 COPI COPI NNP cord-104239-xxlcdbqi 457 7 vesicle vesicle NN cord-104239-xxlcdbqi 457 8 coats coat NNS cord-104239-xxlcdbqi 457 9 in in IN cord-104239-xxlcdbqi 457 10 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 457 11 reticulum reticulum NN cord-104239-xxlcdbqi 457 12 to to IN cord-104239-xxlcdbqi 457 13 Golgi Golgi NNP cord-104239-xxlcdbqi 457 14 transport transport NN cord-104239-xxlcdbqi 457 15 Estimation estimation NN cord-104239-xxlcdbqi 457 16 of of IN cord-104239-xxlcdbqi 457 17 surface surface NN cord-104239-xxlcdbqi 457 18 area area NN cord-104239-xxlcdbqi 457 19 from from IN cord-104239-xxlcdbqi 457 20 vertical vertical JJ cord-104239-xxlcdbqi 457 21 sections section NNS cord-104239-xxlcdbqi 457 22 Reconstitution Reconstitution NNP cord-104239-xxlcdbqi 457 23 of of IN cord-104239-xxlcdbqi 457 24 SEC SEC NNP cord-104239-xxlcdbqi 457 25 gene gene NN cord-104239-xxlcdbqi 457 26 product product NN cord-104239-xxlcdbqi 457 27 - - HYPH cord-104239-xxlcdbqi 457 28 dependent dependent JJ cord-104239-xxlcdbqi 457 29 intercompartmental intercompartmental JJ cord-104239-xxlcdbqi 457 30 protein protein NN cord-104239-xxlcdbqi 457 31 transport transport NN cord-104239-xxlcdbqi 458 1 Beyond beyond IN cord-104239-xxlcdbqi 458 2 bulk bulk JJ cord-104239-xxlcdbqi 458 3 flow flow NN cord-104239-xxlcdbqi 458 4 ATP ATP NNP cord-104239-xxlcdbqi 458 5 - - HYPH cord-104239-xxlcdbqi 458 6 coupled couple VBN cord-104239-xxlcdbqi 458 7 transport transport NN cord-104239-xxlcdbqi 458 8 of of IN cord-104239-xxlcdbqi 458 9 vesicular vesicular JJ cord-104239-xxlcdbqi 458 10 stomatitis stomatitis NN cord-104239-xxlcdbqi 458 11 virus virus NN cord-104239-xxlcdbqi 458 12 G g NN cord-104239-xxlcdbqi 458 13 protein protein NN cord-104239-xxlcdbqi 458 14 between between IN cord-104239-xxlcdbqi 458 15 the the DT cord-104239-xxlcdbqi 458 16 endoplasmie endoplasmie NNP cord-104239-xxlcdbqi 458 17 reticulum reticulum NN cord-104239-xxlcdbqi 458 18 and and CC cord-104239-xxlcdbqi 458 19 the the DT cord-104239-xxlcdbqi 458 20 Golgi Golgi NNP cord-104239-xxlcdbqi 458 21 Vesicular Vesicular NNP cord-104239-xxlcdbqi 458 22 stomatitis stomatitis NN cord-104239-xxlcdbqi 458 23 virus virus NN cord-104239-xxlcdbqi 458 24 glycoprotein glycoprotein NN cord-104239-xxlcdbqi 458 25 is be VBZ cord-104239-xxlcdbqi 458 26 sorted sort VBN cord-104239-xxlcdbqi 458 27 and and CC cord-104239-xxlcdbqi 458 28 concentrated concentrate VBN cord-104239-xxlcdbqi 458 29 during during IN cord-104239-xxlcdbqi 458 30 export export NN cord-104239-xxlcdbqi 458 31 from from IN cord-104239-xxlcdbqi 458 32 the the DT cord-104239-xxlcdbqi 458 33 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 458 34 reticulum reticulum NN cord-104239-xxlcdbqi 459 1 COPII COPII NNP cord-104239-xxlcdbqi 459 2 : : : cord-104239-xxlcdbqi 460 1 a a DT cord-104239-xxlcdbqi 460 2 membrane membrane NN cord-104239-xxlcdbqi 460 3 coat coat NN cord-104239-xxlcdbqi 460 4 that that WDT cord-104239-xxlcdbqi 460 5 forms form VBZ cord-104239-xxlcdbqi 460 6 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 460 7 reticulumderived reticulumderive VBN cord-104239-xxlcdbqi 460 8 vesicles vesicle VBZ cord-104239-xxlcdbqi 460 9 COPII COPII NNP cord-104239-xxlcdbqi 460 10 : : : cord-104239-xxlcdbqi 461 1 a a DT cord-104239-xxlcdbqi 461 2 membrane membrane NN cord-104239-xxlcdbqi 461 3 coat coat NN cord-104239-xxlcdbqi 461 4 formed form VBN cord-104239-xxlcdbqi 461 5 by by IN cord-104239-xxlcdbqi 461 6 sec sec NNP cord-104239-xxlcdbqi 461 7 proteins protein NNS cord-104239-xxlcdbqi 461 8 that that WDT cord-104239-xxlcdbqi 461 9 drive drive VBP cord-104239-xxlcdbqi 461 10 vesicle vesicle NN cord-104239-xxlcdbqi 461 11 budding bud VBG cord-104239-xxlcdbqi 461 12 from from IN cord-104239-xxlcdbqi 461 13 the the DT cord-104239-xxlcdbqi 461 14 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 461 15 reticulum reticulum NN cord-104239-xxlcdbqi 461 16 Semi semi JJ cord-104239-xxlcdbqi 461 17 - - JJ cord-104239-xxlcdbqi 461 18 intact intact JJ cord-104239-xxlcdbqi 461 19 cells cell NNS cord-104239-xxlcdbqi 461 20 permeable permeable JJ cord-104239-xxlcdbqi 461 21 to to IN cord-104239-xxlcdbqi 461 22 macromolecules macromolecule NNS cord-104239-xxlcdbqi 461 23 : : : cord-104239-xxlcdbqi 461 24 use use VB cord-104239-xxlcdbqi 461 25 in in IN cord-104239-xxlcdbqi 461 26 reconstitution reconstitution NN cord-104239-xxlcdbqi 461 27 of of IN cord-104239-xxlcdbqi 461 28 protein protein NN cord-104239-xxlcdbqi 461 29 transport transport NN cord-104239-xxlcdbqi 461 30 from from IN cord-104239-xxlcdbqi 461 31 the the DT cord-104239-xxlcdbqi 461 32 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 461 33 reticulum reticulum NN cord-104239-xxlcdbqi 461 34 to to IN cord-104239-xxlcdbqi 461 35 the the DT cord-104239-xxlcdbqi 461 36 Golgi Golgi NNP cord-104239-xxlcdbqi 461 37 complex complex JJ cord-104239-xxlcdbqi 461 38 Regulation regulation NN cord-104239-xxlcdbqi 461 39 of of IN cord-104239-xxlcdbqi 461 40 constitutive constitutive JJ cord-104239-xxlcdbqi 461 41 exocytic exocytic JJ cord-104239-xxlcdbqi 461 42 transport transport NN cord-104239-xxlcdbqi 461 43 by by IN cord-104239-xxlcdbqi 461 44 membrane membrane NN cord-104239-xxlcdbqi 461 45 receptors receptor NNS cord-104239-xxlcdbqi 461 46 : : : cord-104239-xxlcdbqi 461 47 a a DT cord-104239-xxlcdbqi 461 48 biochemical biochemical JJ cord-104239-xxlcdbqi 461 49 and and CC cord-104239-xxlcdbqi 461 50 morphological morphological JJ cord-104239-xxlcdbqi 461 51 study study NN cord-104239-xxlcdbqi 461 52 Localization localization NN cord-104239-xxlcdbqi 461 53 of of IN cord-104239-xxlcdbqi 461 54 low low JJ cord-104239-xxlcdbqi 461 55 molecular molecular JJ cord-104239-xxlcdbqi 461 56 weight weight NN cord-104239-xxlcdbqi 461 57 GTP GTP NNP cord-104239-xxlcdbqi 461 58 binding bind VBG cord-104239-xxlcdbqi 461 59 proteins protein NNS cord-104239-xxlcdbqi 461 60 to to IN cord-104239-xxlcdbqi 461 61 exocytic exocytic JJ cord-104239-xxlcdbqi 461 62 and and CC cord-104239-xxlcdbqi 461 63 endocytic endocytic JJ cord-104239-xxlcdbqi 461 64 compartments compartment NNS cord-104239-xxlcdbqi 461 65 Transport Transport NNP cord-104239-xxlcdbqi 461 66 into into IN cord-104239-xxlcdbqi 461 67 and and CC cord-104239-xxlcdbqi 461 68 out out IN cord-104239-xxlcdbqi 461 69 of of IN cord-104239-xxlcdbqi 461 70 the the DT cord-104239-xxlcdbqi 461 71 Golgi Golgi NNP cord-104239-xxlcdbqi 461 72 complex complex NN cord-104239-xxlcdbqi 461 73 studied study VBN cord-104239-xxlcdbqi 461 74 by by IN cord-104239-xxlcdbqi 461 75 transfecting transfecte VBG cord-104239-xxlcdbqi 461 76 cells cell NNS cord-104239-xxlcdbqi 461 77 with with IN cord-104239-xxlcdbqi 461 78 cDNAs cdna NNS cord-104239-xxlcdbqi 461 79 encoding encode VBG cord-104239-xxlcdbqi 461 80 horseradish horseradish NN cord-104239-xxlcdbqi 461 81 peroxidase peroxidase NN cord-104239-xxlcdbqi 461 82 Differential differential JJ cord-104239-xxlcdbqi 461 83 inhibition inhibition NN cord-104239-xxlcdbqi 461 84 of of IN cord-104239-xxlcdbqi 461 85 multiple multiple JJ cord-104239-xxlcdbqi 461 86 vesicular vesicular JJ cord-104239-xxlcdbqi 461 87 transport transport NN cord-104239-xxlcdbqi 461 88 steps step NNS cord-104239-xxlcdbqi 461 89 between between IN cord-104239-xxlcdbqi 461 90 the the DT cord-104239-xxlcdbqi 461 91 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 461 92 reticulum reticulum NN cord-104239-xxlcdbqi 461 93 and and CC cord-104239-xxlcdbqi 461 94 trans trans NNP cord-104239-xxlcdbqi 461 95 - - JJ cord-104239-xxlcdbqi 461 96 Golgi Golgi NNP cord-104239-xxlcdbqi 461 97 network network NN cord-104239-xxlcdbqi 461 98 Density density NN cord-104239-xxlcdbqi 461 99 of of IN cord-104239-xxlcdbqi 461 100 newly newly RB cord-104239-xxlcdbqi 461 101 synthesized synthesize VBN cord-104239-xxlcdbqi 461 102 plasma plasma NN cord-104239-xxlcdbqi 461 103 membrane membrane NN cord-104239-xxlcdbqi 461 104 proteins protein NNS cord-104239-xxlcdbqi 461 105 in in IN cord-104239-xxlcdbqi 461 106 intracellular intracellular JJ cord-104239-xxlcdbqi 461 107 membranes membrane NNS cord-104239-xxlcdbqi 461 108 . . . cord-104239-xxlcdbqi 462 1 I. I. NNP cord-104239-xxlcdbqi 462 2 Stereological Stereological NNP cord-104239-xxlcdbqi 462 3 studies study VBZ cord-104239-xxlcdbqi 462 4 The the DT cord-104239-xxlcdbqi 462 5 dynamic dynamic JJ cord-104239-xxlcdbqi 462 6 nature nature NN cord-104239-xxlcdbqi 462 7 of of IN cord-104239-xxlcdbqi 462 8 the the DT cord-104239-xxlcdbqi 462 9 Golgi Golgi NNP cord-104239-xxlcdbqi 462 10 complex complex NN cord-104239-xxlcdbqi 463 1 The the DT cord-104239-xxlcdbqi 463 2 bulk bulk JJ cord-104239-xxlcdbqi 463 3 - - HYPH cord-104239-xxlcdbqi 463 4 flow flow NN cord-104239-xxlcdbqi 463 5 hypothesis hypothesis NN cord-104239-xxlcdbqi 463 6 : : : cord-104239-xxlcdbqi 463 7 not not RB cord-104239-xxlcdbqi 463 8 quite quite PDT cord-104239-xxlcdbqi 463 9 the the DT cord-104239-xxlcdbqi 463 10 end end NN cord-104239-xxlcdbqi 464 1 Immunocytochemical Immunocytochemical NNP cord-104239-xxlcdbqi 464 2 localization localization NN cord-104239-xxlcdbqi 464 3 of of IN cord-104239-xxlcdbqi 464 4 [ [ -LRB- cord-104239-xxlcdbqi 464 5 3-COP 3-COP NNP cord-104239-xxlcdbqi 464 6 to to IN cord-104239-xxlcdbqi 464 7 the the DT cord-104239-xxlcdbqi 464 8 ER ER NNP cord-104239-xxlcdbqi 464 9 - - HYPH cord-104239-xxlcdbqi 464 10 Golgi Golgi NNP cord-104239-xxlcdbqi 464 11 boundary boundary NN cord-104239-xxlcdbqi 464 12 and and CC cord-104239-xxlcdbqi 464 13 the the DT cord-104239-xxlcdbqi 464 14 TGN TGN NNP cord-104239-xxlcdbqi 464 15 Disruption Disruption NNP cord-104239-xxlcdbqi 464 16 of of IN cord-104239-xxlcdbqi 464 17 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 464 18 reticulum reticulum NN cord-104239-xxlcdbqi 464 19 to to IN cord-104239-xxlcdbqi 464 20 Golgi Golgi NNP cord-104239-xxlcdbqi 464 21 transport transport NN cord-104239-xxlcdbqi 464 22 leads lead VBZ cord-104239-xxlcdbqi 464 23 to to IN cord-104239-xxlcdbqi 464 24 the the DT cord-104239-xxlcdbqi 464 25 accumulation accumulation NN cord-104239-xxlcdbqi 464 26 of of IN cord-104239-xxlcdbqi 464 27 large large JJ cord-104239-xxlcdbqi 464 28 aggregates aggregate NNS cord-104239-xxlcdbqi 464 29 containing contain VBG cord-104239-xxlcdbqi 464 30 13-COP 13-cop CD cord-104239-xxlcdbqi 464 31 in in IN cord-104239-xxlcdbqi 464 32 pancreatic pancreatic JJ cord-104239-xxlcdbqi 464 33 acinar acinar NN cord-104239-xxlcdbqi 464 34 cells cell NNS cord-104239-xxlcdbqi 465 1 Three three CD cord-104239-xxlcdbqi 465 2 - - HYPH cord-104239-xxlcdbqi 465 3 dimensional dimensional JJ cord-104239-xxlcdbqi 465 4 visualization visualization NN cord-104239-xxlcdbqi 465 5 of of IN cord-104239-xxlcdbqi 465 6 coated coated JJ cord-104239-xxlcdbqi 465 7 vesicle vesicle NN cord-104239-xxlcdbqi 465 8 formation formation NN cord-104239-xxlcdbqi 465 9 in in IN cord-104239-xxlcdbqi 465 10 fibroblasts fibroblast NNS cord-104239-xxlcdbqi 466 1 Microinjected Microinjected NNP cord-104239-xxlcdbqi 466 2 antibodies antibody NNS cord-104239-xxlcdbqi 466 3 against against IN cord-104239-xxlcdbqi 466 4 the the DT cord-104239-xxlcdbqi 466 5 cytoplasmic cytoplasmic JJ cord-104239-xxlcdbqi 466 6 domain domain NN cord-104239-xxlcdbqi 466 7 of of IN cord-104239-xxlcdbqi 466 8 vesicular vesicular JJ cord-104239-xxlcdbqi 466 9 stomatitis stomatitis NN cord-104239-xxlcdbqi 466 10 virus virus NN cord-104239-xxlcdbqi 466 11 glycoprotein glycoprotein NN cord-104239-xxlcdbqi 466 12 block block VB cord-104239-xxlcdbqi 466 13 its -PRON- PRP$ cord-104239-xxlcdbqi 466 14 transport transport NN cord-104239-xxlcdbqi 466 15 to to IN cord-104239-xxlcdbqi 466 16 the the DT cord-104239-xxlcdbqi 466 17 cell cell NN cord-104239-xxlcdbqi 466 18 surface surface NN cord-104239-xxlcdbqi 466 19 Characterization characterization NN cord-104239-xxlcdbqi 466 20 of of IN cord-104239-xxlcdbqi 466 21 the the DT cord-104239-xxlcdbqi 466 22 budding bud VBG cord-104239-xxlcdbqi 466 23 compartment compartment NN cord-104239-xxlcdbqi 466 24 of of IN cord-104239-xxlcdbqi 466 25 mouse mouse NNP cord-104239-xxlcdbqi 466 26 hepatitis hepatitis NNP cord-104239-xxlcdbqi 466 27 virus virus NN cord-104239-xxlcdbqi 466 28 : : : cord-104239-xxlcdbqi 466 29 evidence evidence NN cord-104239-xxlcdbqi 466 30 that that DT cord-104239-xxlcdbqi 466 31 transport transport NN cord-104239-xxlcdbqi 466 32 from from IN cord-104239-xxlcdbqi 466 33 the the DT cord-104239-xxlcdbqi 466 34 RER RER NNP cord-104239-xxlcdbqi 466 35 to to IN cord-104239-xxlcdbqi 466 36 the the DT cord-104239-xxlcdbqi 466 37 Golgi Golgi NNP cord-104239-xxlcdbqi 466 38 complex complex NN cord-104239-xxlcdbqi 466 39 requires require VBZ cord-104239-xxlcdbqi 466 40 only only RB cord-104239-xxlcdbqi 466 41 one one CD cord-104239-xxlcdbqi 466 42 vesicular vesicular JJ cord-104239-xxlcdbqi 466 43 transport transport NN cord-104239-xxlcdbqi 466 44 step step NN cord-104239-xxlcdbqi 466 45 Sarl Sarl NNP cord-104239-xxlcdbqi 466 46 promotes promote VBZ cord-104239-xxlcdbqi 466 47 vesicle vesicle NN cord-104239-xxlcdbqi 466 48 budding bud VBG cord-104239-xxlcdbqi 466 49 from from IN cord-104239-xxlcdbqi 466 50 the the DT cord-104239-xxlcdbqi 466 51 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 466 52 reticulum reticulum NN cord-104239-xxlcdbqi 466 53 but but CC cord-104239-xxlcdbqi 466 54 not not RB cord-104239-xxlcdbqi 466 55 Golgi Golgi NNP cord-104239-xxlcdbqi 466 56 compartments compartment NNS cord-104239-xxlcdbqi 466 57 Envelope envelope NN cord-104239-xxlcdbqi 466 58 viruses virus NNS cord-104239-xxlcdbqi 466 59 of of IN cord-104239-xxlcdbqi 466 60 vesicular vesicular JJ cord-104239-xxlcdbqi 466 61 stomatitis stomatitis NN cord-104239-xxlcdbqi 466 62 virus virus NN cord-104239-xxlcdbqi 466 63 : : : cord-104239-xxlcdbqi 467 1 effect effect NN cord-104239-xxlcdbqi 467 2 of of IN cord-104239-xxlcdbqi 467 3 temperature temperature NN cord-104239-xxlcdbqi 467 4 - - HYPH cord-104239-xxlcdbqi 467 5 sensitive sensitive JJ cord-104239-xxlcdbqi 467 6 mutations mutation NNS cord-104239-xxlcdbqi 467 7 in in IN cord-104239-xxlcdbqi 467 8 complementation complementation NN cord-104239-xxlcdbqi 467 9 groups group NNS cord-104239-xxlcdbqi 467 10 III III NNP cord-104239-xxlcdbqi 467 11 and and CC cord-104239-xxlcdbqi 467 12 V V NNP cord-104239-xxlcdbqi 467 13 Coatomer Coatomer NNP cord-104239-xxlcdbqi 467 14 is be VBZ cord-104239-xxlcdbqi 467 15 essential essential JJ cord-104239-xxlcdbqi 467 16 for for IN cord-104239-xxlcdbqi 467 17 retrieval retrieval NN cord-104239-xxlcdbqi 467 18 of of IN cord-104239-xxlcdbqi 467 19 dilysine dilysine NN cord-104239-xxlcdbqi 467 20 - - HYPH cord-104239-xxlcdbqi 467 21 tagged tag VBN cord-104239-xxlcdbqi 467 22 proteins protein NNS cord-104239-xxlcdbqi 467 23 to to IN cord-104239-xxlcdbqi 467 24 the the DT cord-104239-xxlcdbqi 467 25 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 467 26 reticulum reticulum NN cord-104239-xxlcdbqi 467 27 Bi bi JJ cord-104239-xxlcdbqi 467 28 - - HYPH cord-104239-xxlcdbqi 467 29 directional directional JJ cord-104239-xxlcdbqi 467 30 membrane membrane NN cord-104239-xxlcdbqi 467 31 traffic traffic NN cord-104239-xxlcdbqi 467 32 between between IN cord-104239-xxlcdbqi 467 33 the the DT cord-104239-xxlcdbqi 467 34 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 467 35 reticulum reticulum NN cord-104239-xxlcdbqi 467 36 and and CC cord-104239-xxlcdbqi 467 37 Golgi Golgi NNP cord-104239-xxlcdbqi 467 38 apparatus apparatus NN cord-104239-xxlcdbqi 467 39 Kinesin Kinesin NNP cord-104239-xxlcdbqi 467 40 is be VBZ cord-104239-xxlcdbqi 467 41 the the DT cord-104239-xxlcdbqi 467 42 motor motor NN cord-104239-xxlcdbqi 467 43 for for IN cord-104239-xxlcdbqi 467 44 microtubule microtubule NN cord-104239-xxlcdbqi 467 45 - - HYPH cord-104239-xxlcdbqi 467 46 mediated mediate VBN cord-104239-xxlcdbqi 467 47 Golgi golgi NN cord-104239-xxlcdbqi 467 48 - - HYPH cord-104239-xxlcdbqi 467 49 to to IN cord-104239-xxlcdbqi 467 50 - - HYPH cord-104239-xxlcdbqi 467 51 ER er NN cord-104239-xxlcdbqi 467 52 membrane membrane NN cord-104239-xxlcdbqi 467 53 traffic traffic NN cord-104239-xxlcdbqi 467 54 Immunocytochemical Immunocytochemical NNP cord-104239-xxlcdbqi 467 55 analysis analysis NN cord-104239-xxlcdbqi 467 56 of of IN cord-104239-xxlcdbqi 467 57 the the DT cord-104239-xxlcdbqi 467 58 transfer transfer NN cord-104239-xxlcdbqi 467 59 of of IN cord-104239-xxlcdbqi 467 60 vesicular vesicular JJ cord-104239-xxlcdbqi 467 61 stomatitis stomatitis NN cord-104239-xxlcdbqi 467 62 virus virus NN cord-104239-xxlcdbqi 467 63 G g NN cord-104239-xxlcdbqi 467 64 glycoprotein glycoprotein NN cord-104239-xxlcdbqi 467 65 from from IN cord-104239-xxlcdbqi 467 66 the the DT cord-104239-xxlcdbqi 467 67 intermediate intermediate JJ cord-104239-xxlcdbqi 467 68 compartment compartment NN cord-104239-xxlcdbqi 467 69 to to IN cord-104239-xxlcdbqi 467 70 the the DT cord-104239-xxlcdbqi 467 71 Golgi Golgi NNP cord-104239-xxlcdbqi 467 72 complex complex NN cord-104239-xxlcdbqi 467 73 Mitotic mitotic JJ cord-104239-xxlcdbqi 467 74 Golgi Golgi NNP cord-104239-xxlcdbqi 467 75 fragments fragment NNS cord-104239-xxlcdbqi 467 76 in in IN cord-104239-xxlcdbqi 467 77 HeLa HeLa NNP cord-104239-xxlcdbqi 467 78 cells cell NNS cord-104239-xxlcdbqi 467 79 and and CC cord-104239-xxlcdbqi 467 80 their -PRON- PRP$ cord-104239-xxlcdbqi 467 81 role role NN cord-104239-xxlcdbqi 467 82 in in IN cord-104239-xxlcdbqi 467 83 the the DT cord-104239-xxlcdbqi 467 84 reassembly reassembly NN cord-104239-xxlcdbqi 467 85 pathway pathway NN cord-104239-xxlcdbqi 468 1 The the DT cord-104239-xxlcdbqi 468 2 Gotgi Gotgi NNP cord-104239-xxlcdbqi 468 3 complex complex NN cord-104239-xxlcdbqi 468 4 : : : cord-104239-xxlcdbqi 468 5 in in FW cord-104239-xxlcdbqi 468 6 vitro vitro FW cord-104239-xxlcdbqi 468 7 veritas veritas FW cord-104239-xxlcdbqi 468 8 ? ? . cord-104239-xxlcdbqi 469 1 Celt Celt NNP cord-104239-xxlcdbqi 470 1 The the DT cord-104239-xxlcdbqi 470 2 reorganization reorganization NN cord-104239-xxlcdbqi 470 3 of of IN cord-104239-xxlcdbqi 470 4 the the DT cord-104239-xxlcdbqi 470 5 Golgi Golgi NNP cord-104239-xxlcdbqi 470 6 complex complex NN cord-104239-xxlcdbqi 470 7 in in IN cord-104239-xxlcdbqi 470 8 anoxic anoxic JJ cord-104239-xxlcdbqi 470 9 pancreatic pancreatic JJ cord-104239-xxlcdbqi 470 10 acinar acinar NN cord-104239-xxlcdbqi 470 11 ceils ceils NNP cord-104239-xxlcdbqi 470 12 Mammalian Mammalian NNP cord-104239-xxlcdbqi 470 13 Sec23p Sec23p NNP cord-104239-xxlcdbqi 470 14 homologue homologue NN cord-104239-xxlcdbqi 470 15 is be VBZ cord-104239-xxlcdbqi 470 16 restricted restrict VBN cord-104239-xxlcdbqi 470 17 to to IN cord-104239-xxlcdbqi 470 18 the the DT cord-104239-xxlcdbqi 470 19 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 470 20 reticulum reticulum NN cord-104239-xxlcdbqi 470 21 transitional transitional JJ cord-104239-xxlcdbqi 470 22 cytoplasm cytoplasm NN cord-104239-xxlcdbqi 470 23 Coated Coated NNP cord-104239-xxlcdbqi 470 24 vesicle vesicle NN cord-104239-xxlcdbqi 470 25 assembly assembly NN cord-104239-xxlcdbqi 470 26 in in IN cord-104239-xxlcdbqi 470 27 the the DT cord-104239-xxlcdbqi 470 28 Golgi Golgi NNP cord-104239-xxlcdbqi 470 29 requires require VBZ cord-104239-xxlcdbqi 470 30 only only RB cord-104239-xxlcdbqi 470 31 coatomer coatomer NN cord-104239-xxlcdbqi 470 32 and and CC cord-104239-xxlcdbqi 470 33 ARF ARF NNP cord-104239-xxlcdbqi 470 34 proteins protein NNS cord-104239-xxlcdbqi 470 35 from from IN cord-104239-xxlcdbqi 470 36 the the DT cord-104239-xxlcdbqi 470 37 cytosol cytosol NN cord-104239-xxlcdbqi 470 38 BFA BFA NNP cord-104239-xxlcdbqi 470 39 bodies body NNS cord-104239-xxlcdbqi 470 40 : : : cord-104239-xxlcdbqi 470 41 a a DT cord-104239-xxlcdbqi 470 42 subcompartment subcompartment NN cord-104239-xxlcdbqi 470 43 of of IN cord-104239-xxlcdbqi 470 44 the the DT cord-104239-xxlcdbqi 470 45 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 470 46 reticulum reticulum NN cord-104239-xxlcdbqi 470 47 lntracellular lntracellular JJ cord-104239-xxlcdbqi 470 48 aspects aspect NNS cord-104239-xxlcdbqi 470 49 of of IN cord-104239-xxlcdbqi 470 50 the the DT cord-104239-xxlcdbqi 470 51 process process NN cord-104239-xxlcdbqi 470 52 of of IN cord-104239-xxlcdbqi 470 53 protein protein NN cord-104239-xxlcdbqi 470 54 transport transport NN cord-104239-xxlcdbqi 470 55 . . . cord-104239-xxlcdbqi 471 1 Science science NN cord-104239-xxlcdbqi 471 2 ( ( -LRB- cord-104239-xxlcdbqi 471 3 Wash. Washington NNP cord-104239-xxlcdbqi 471 4 DC DC NNP cord-104239-xxlcdbqi 471 5 ) ) -RRB- cord-104239-xxlcdbqi 472 1 Evidence evidence NN cord-104239-xxlcdbqi 472 2 that that IN cord-104239-xxlcdbqi 472 3 luminal luminal JJ cord-104239-xxlcdbqi 472 4 ER er NN cord-104239-xxlcdbqi 472 5 proteins protein NNS cord-104239-xxlcdbqi 472 6 are be VBP cord-104239-xxlcdbqi 472 7 sorted sort VBN cord-104239-xxlcdbqi 472 8 from from IN cord-104239-xxlcdbqi 472 9 secreted secreted JJ cord-104239-xxlcdbqi 472 10 proteins protein NNS cord-104239-xxlcdbqi 472 11 in in IN cord-104239-xxlcdbqi 472 12 a a DT cord-104239-xxlcdbqi 472 13 post post JJ cord-104239-xxlcdbqi 472 14 - - JJ cord-104239-xxlcdbqi 472 15 ER er JJ cord-104239-xxlcdbqi 472 16 compartment compartment NN cord-104239-xxlcdbqi 473 1 13-COP 13-cop CD cord-104239-xxlcdbqi 473 2 is be VBZ cord-104239-xxlcdbqi 473 3 essential essential JJ cord-104239-xxlcdbqi 473 4 for for IN cord-104239-xxlcdbqi 473 5 biosynthetic biosynthetic JJ cord-104239-xxlcdbqi 473 6 membrane membrane NN cord-104239-xxlcdbqi 473 7 transport transport NN cord-104239-xxlcdbqi 473 8 from from IN cord-104239-xxlcdbqi 473 9 the the DT cord-104239-xxlcdbqi 473 10 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 473 11 reticulum reticulum NN cord-104239-xxlcdbqi 473 12 to to IN cord-104239-xxlcdbqi 473 13 the the DT cord-104239-xxlcdbqi 473 14 Golgi Golgi NNP cord-104239-xxlcdbqi 473 15 complex complex NN cord-104239-xxlcdbqi 473 16 in in IN cord-104239-xxlcdbqi 473 17 vivo vivo NNP cord-104239-xxlcdbqi 473 18 13-COP 13-cop CD cord-104239-xxlcdbqi 473 19 is be VBZ cord-104239-xxlcdbqi 473 20 essential essential JJ cord-104239-xxlcdbqi 473 21 for for IN cord-104239-xxlcdbqi 473 22 transport transport NN cord-104239-xxlcdbqi 473 23 of of IN cord-104239-xxlcdbqi 473 24 protein protein NN cord-104239-xxlcdbqi 473 25 from from IN cord-104239-xxlcdbqi 473 26 the the DT cord-104239-xxlcdbqi 473 27 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 473 28 reticulum reticulum NN cord-104239-xxlcdbqi 473 29 to to IN cord-104239-xxlcdbqi 473 30 the the DT cord-104239-xxlcdbqi 473 31 Golgi Golgi NNP cord-104239-xxlcdbqi 474 1 in in IN cord-104239-xxlcdbqi 474 2 vitro vitro FW cord-104239-xxlcdbqi 475 1 Rabl Rabl NNP cord-104239-xxlcdbqi 475 2 and and CC cord-104239-xxlcdbqi 475 3 Ca Ca NNP cord-104239-xxlcdbqi 475 4 2 2 CD cord-104239-xxlcdbqi 475 5 + + NFP cord-104239-xxlcdbqi 475 6 are be VBP cord-104239-xxlcdbqi 475 7 required require VBN cord-104239-xxlcdbqi 475 8 for for IN cord-104239-xxlcdbqi 475 9 the the DT cord-104239-xxlcdbqi 475 10 fusion fusion NN cord-104239-xxlcdbqi 475 11 of of IN cord-104239-xxlcdbqi 475 12 carrier carrier NN cord-104239-xxlcdbqi 475 13 vesicles vesicle NNS cord-104239-xxlcdbqi 475 14 mediating mediate VBG cord-104239-xxlcdbqi 475 15 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 475 16 reticulum reticulum NN cord-104239-xxlcdbqi 475 17 to to IN cord-104239-xxlcdbqi 475 18 Golgi Golgi NNP cord-104239-xxlcdbqi 475 19 transport transport NN cord-104239-xxlcdbqi 475 20 Participation participation NN cord-104239-xxlcdbqi 475 21 of of IN cord-104239-xxlcdbqi 475 22 the the DT cord-104239-xxlcdbqi 475 23 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 476 1 reticulum reticulum NN cord-104239-xxlcdbqi 476 2 chaperone chaperone NN cord-104239-xxlcdbqi 476 3 calnexin calnexin NN cord-104239-xxlcdbqi 476 4 ( ( -LRB- cord-104239-xxlcdbqi 476 5 p88 p88 NNP cord-104239-xxlcdbqi 476 6 , , , cord-104239-xxlcdbqi 476 7 IP90 IP90 NNP cord-104239-xxlcdbqi 476 8 ) ) -RRB- cord-104239-xxlcdbqi 476 9 in in IN cord-104239-xxlcdbqi 476 10 the the DT cord-104239-xxlcdbqi 476 11 biogenesis biogenesis NN cord-104239-xxlcdbqi 476 12 of of IN cord-104239-xxlcdbqi 476 13 the the DT cord-104239-xxlcdbqi 476 14 cystic cystic JJ cord-104239-xxlcdbqi 476 15 fibrosis fibrosis NN cord-104239-xxlcdbqi 476 16 transmembrane transmembrane NNP cord-104239-xxlcdbqi 476 17 conductance conductance NN cord-104239-xxlcdbqi 476 18 regulator regulator NN cord-104239-xxlcdbqi 476 19 Morphological morphological JJ cord-104239-xxlcdbqi 476 20 analysis analysis NN cord-104239-xxlcdbqi 476 21 of of IN cord-104239-xxlcdbqi 476 22 protein protein NN cord-104239-xxlcdbqi 476 23 transport transport NN cord-104239-xxlcdbqi 476 24 from from IN cord-104239-xxlcdbqi 476 25 the the DT cord-104239-xxlcdbqi 476 26 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 476 27 reticulum reticulum NN cord-104239-xxlcdbqi 476 28 to to IN cord-104239-xxlcdbqi 476 29 Golgi Golgi NNP cord-104239-xxlcdbqi 476 30 membranes membrane NNS cord-104239-xxlcdbqi 476 31 in in IN cord-104239-xxlcdbqi 476 32 digitonin digitonin NN cord-104239-xxlcdbqi 476 33 - - HYPH cord-104239-xxlcdbqi 476 34 permeabilized permeabilize VBN cord-104239-xxlcdbqi 476 35 cells cell NNS cord-104239-xxlcdbqi 476 36 : : : cord-104239-xxlcdbqi 476 37 role role NN cord-104239-xxlcdbqi 476 38 of of IN cord-104239-xxlcdbqi 476 39 the the DT cord-104239-xxlcdbqi 476 40 p58 p58 NNP cord-104239-xxlcdbqi 476 41 containing contain VBG cord-104239-xxlcdbqi 476 42 compartment compartment NN cord-104239-xxlcdbqi 476 43 Biosynthesis Biosynthesis NNP cord-104239-xxlcdbqi 476 44 and and CC cord-104239-xxlcdbqi 476 45 intracellular intracellular JJ cord-104239-xxlcdbqi 476 46 sorting sorting NN cord-104239-xxlcdbqi 476 47 of of IN cord-104239-xxlcdbqi 476 48 growth growth NN cord-104239-xxlcdbqi 476 49 hormone hormone NN cord-104239-xxlcdbqi 476 50 - - HYPH cord-104239-xxlcdbqi 476 51 viral viral JJ cord-104239-xxlcdbqi 476 52 envelope envelope NN cord-104239-xxlcdbqi 476 53 glycoprotein glycoprotein NN cord-104239-xxlcdbqi 476 54 hybrids hybrid NNS cord-104239-xxlcdbqi 476 55 Pre pre VB cord-104239-xxlcdbqi 476 56 - - : cord-104239-xxlcdbqi 476 57 and and CC cord-104239-xxlcdbqi 476 58 post post JJ cord-104239-xxlcdbqi 476 59 - - JJ cord-104239-xxlcdbqi 476 60 Golgi golgi JJ cord-104239-xxlcdbqi 476 61 vacuoles vacuole NNS cord-104239-xxlcdbqi 476 62 operate operate VBP cord-104239-xxlcdbqi 476 63 in in IN cord-104239-xxlcdbqi 476 64 the the DT cord-104239-xxlcdbqi 476 65 transport transport NN cord-104239-xxlcdbqi 476 66 of of IN cord-104239-xxlcdbqi 476 67 Semliki Semliki NNP cord-104239-xxlcdbqi 476 68 Forest Forest NNP cord-104239-xxlcdbqi 476 69 virus virus NN cord-104239-xxlcdbqi 476 70 membrane membrane NN cord-104239-xxlcdbqi 476 71 glycoproteins glycoprotein NNS cord-104239-xxlcdbqi 476 72 to to IN cord-104239-xxlcdbqi 476 73 the the DT cord-104239-xxlcdbqi 476 74 ceil ceil NN cord-104239-xxlcdbqi 476 75 surface surface NN cord-104239-xxlcdbqi 476 76 Distribution distribution NN cord-104239-xxlcdbqi 476 77 of of IN cord-104239-xxlcdbqi 476 78 the the DT cord-104239-xxlcdbqi 476 79 intermediate intermediate JJ cord-104239-xxlcdbqi 476 80 elements element NNS cord-104239-xxlcdbqi 476 81 operating operate VBG cord-104239-xxlcdbqi 476 82 in in IN cord-104239-xxlcdbqi 476 83 ER ER NNP cord-104239-xxlcdbqi 476 84 to to IN cord-104239-xxlcdbqi 476 85 Golgi Golgi NNP cord-104239-xxlcdbqi 476 86 transport transport NN cord-104239-xxlcdbqi 476 87 Identification identification NN cord-104239-xxlcdbqi 476 88 , , , cord-104239-xxlcdbqi 476 89 by by IN cord-104239-xxlcdbqi 476 90 a a DT cord-104239-xxlcdbqi 476 91 monoclonal monoclonal JJ cord-104239-xxlcdbqi 476 92 antibody antibody NN cord-104239-xxlcdbqi 476 93 , , , cord-104239-xxlcdbqi 476 94 of of IN cord-104239-xxlcdbqi 476 95 a a DT cord-104239-xxlcdbqi 476 96 53-kD 53-kd CD cord-104239-xxlcdbqi 476 97 protein protein NN cord-104239-xxlcdbqi 476 98 associated associate VBN cord-104239-xxlcdbqi 476 99 with with IN cord-104239-xxlcdbqi 476 100 a a DT cord-104239-xxlcdbqi 476 101 tubulo tubulo JJ cord-104239-xxlcdbqi 476 102 - - HYPH cord-104239-xxlcdbqi 476 103 vesicular vesicular JJ cord-104239-xxlcdbqi 476 104 compartment compartment NN cord-104239-xxlcdbqi 476 105 at at IN cord-104239-xxlcdbqi 476 106 the the DT cord-104239-xxlcdbqi 476 107 cis cis NNP cord-104239-xxlcdbqi 476 108 - - HYPH cord-104239-xxlcdbqi 476 109 side side NN cord-104239-xxlcdbqi 476 110 of of IN cord-104239-xxlcdbqi 476 111 the the DT cord-104239-xxlcdbqi 476 112 Golgi Golgi NNP cord-104239-xxlcdbqi 476 113 apparatus apparatus NN cord-104239-xxlcdbqi 476 114 Identification identification NN cord-104239-xxlcdbqi 476 115 of of IN cord-104239-xxlcdbqi 476 116 an an DT cord-104239-xxlcdbqi 476 117 intermediate intermediate JJ cord-104239-xxlcdbqi 476 118 compartment compartment NN cord-104239-xxlcdbqi 476 119 involved involve VBN cord-104239-xxlcdbqi 476 120 in in IN cord-104239-xxlcdbqi 476 121 protein protein NN cord-104239-xxlcdbqi 476 122 transport transport NN cord-104239-xxlcdbqi 476 123 from from IN cord-104239-xxlcdbqi 476 124 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 476 125 reticulum reticulum NN cord-104239-xxlcdbqi 476 126 to to IN cord-104239-xxlcdbqi 476 127 Golgi Golgi NNP cord-104239-xxlcdbqi 476 128 apparatus apparatus NN cord-104239-xxlcdbqi 476 129 A a DT cord-104239-xxlcdbqi 476 130 threedimensional threedimensional JJ cord-104239-xxlcdbqi 476 131 reconstruction reconstruction NN cord-104239-xxlcdbqi 476 132 study study NN cord-104239-xxlcdbqi 476 133 of of IN cord-104239-xxlcdbqi 476 134 the the DT cord-104239-xxlcdbqi 476 135 rough rough JJ cord-104239-xxlcdbqi 476 136 ER ER NNP cord-104239-xxlcdbqi 476 137 - - HYPH cord-104239-xxlcdbqi 476 138 Golgi golgi NN cord-104239-xxlcdbqi 476 139 interface interface NN cord-104239-xxlcdbqi 476 140 in in IN cord-104239-xxlcdbqi 476 141 serial serial JJ cord-104239-xxlcdbqi 476 142 thin thin JJ cord-104239-xxlcdbqi 476 143 sections section NNS cord-104239-xxlcdbqi 476 144 of of IN cord-104239-xxlcdbqi 476 145 the the DT cord-104239-xxlcdbqi 476 146 pancreatic pancreatic JJ cord-104239-xxlcdbqi 476 147 acinar acinar JJ cord-104239-xxlcdbqi 476 148 cell cell NN cord-104239-xxlcdbqi 476 149 of of IN cord-104239-xxlcdbqi 476 150 the the DT cord-104239-xxlcdbqi 476 151 rat rat NN cord-104239-xxlcdbqi 476 152 Human Human NNP cord-104239-xxlcdbqi 476 153 SEC13Rp sec13rp NN cord-104239-xxlcdbqi 476 154 functions function NNS cord-104239-xxlcdbqi 476 155 in in IN cord-104239-xxlcdbqi 476 156 yeast yeast NN cord-104239-xxlcdbqi 476 157 and and CC cord-104239-xxlcdbqi 476 158 is be VBZ cord-104239-xxlcdbqi 476 159 located locate VBN cord-104239-xxlcdbqi 476 160 on on IN cord-104239-xxlcdbqi 476 161 transport transport NN cord-104239-xxlcdbqi 476 162 vesicles vesicle NNS cord-104239-xxlcdbqi 476 163 budding bud VBG cord-104239-xxlcdbqi 476 164 from from IN cord-104239-xxlcdbqi 476 165 the the DT cord-104239-xxlcdbqi 476 166 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 476 167 reticulum reticulum NN cord-104239-xxlcdbqi 476 168 Estimating estimate VBG cord-104239-xxlcdbqi 476 169 number number NN cord-104239-xxlcdbqi 476 170 , , , cord-104239-xxlcdbqi 476 171 mean mean JJ cord-104239-xxlcdbqi 476 172 sizes size NNS cord-104239-xxlcdbqi 476 173 and and CC cord-104239-xxlcdbqi 476 174 variations variation NNS cord-104239-xxlcdbqi 476 175 in in IN cord-104239-xxlcdbqi 476 176 size size NN cord-104239-xxlcdbqi 476 177 of of IN cord-104239-xxlcdbqi 476 178 particles particle NNS cord-104239-xxlcdbqi 476 179 in in IN cord-104239-xxlcdbqi 476 180 3-D 3-d JJ cord-104239-xxlcdbqi 476 181 specimens specimen NNS cord-104239-xxlcdbqi 476 182 using use VBG cord-104239-xxlcdbqi 476 183 disectors disector NNS cord-104239-xxlcdbqi 476 184 Anterograde anterograde JJ cord-104239-xxlcdbqi 476 185 and and CC cord-104239-xxlcdbqi 476 186 retrograde retrograde JJ cord-104239-xxlcdbqi 476 187 traffic traffic NN cord-104239-xxlcdbqi 476 188 between between IN cord-104239-xxlcdbqi 476 189 the the DT cord-104239-xxlcdbqi 476 190 rough rough JJ cord-104239-xxlcdbqi 476 191 endoplasmic endoplasmic NN cord-104239-xxlcdbqi 476 192 reticulum reticulum NN cord-104239-xxlcdbqi 476 193 and and CC cord-104239-xxlcdbqi 476 194 the the DT cord-104239-xxlcdbqi 476 195 Golgi Golgi NNP cord-104239-xxlcdbqi 476 196 complex complex JJ cord-104239-xxlcdbqi 476 197 Segregation segregation NN cord-104239-xxlcdbqi 476 198 of of IN cord-104239-xxlcdbqi 476 199 ERGIC53 ERGIC53 NNP cord-104239-xxlcdbqi 476 200 and and CC cord-104239-xxlcdbqi 476 201 the the DT cord-104239-xxlcdbqi 476 202 mammalian mammalian JJ cord-104239-xxlcdbqi 476 203 KDEL KDEL NNP cord-104239-xxlcdbqi 476 204 receptor receptor NN cord-104239-xxlcdbqi 476 205 upon upon IN cord-104239-xxlcdbqi 476 206 exit exit NN cord-104239-xxlcdbqi 476 207 from from IN cord-104239-xxlcdbqi 476 208 the the DT cord-104239-xxlcdbqi 476 209 15 15 CD cord-104239-xxlcdbqi 476 210 ° ° NN cord-104239-xxlcdbqi 476 211 C C NNP cord-104239-xxlcdbqi 476 212 compartment compartment NN cord-104239-xxlcdbqi 476 213 Replication replication NN cord-104239-xxlcdbqi 476 214 of of IN cord-104239-xxlcdbqi 476 215 coronavirus coronavirus NN cord-104239-xxlcdbqi 476 216 MHV mhv NN cord-104239-xxlcdbqi 476 217 - - HYPH cord-104239-xxlcdbqi 476 218 A59 A59 NNP cord-104239-xxlcdbqi 476 219 in in IN cord-104239-xxlcdbqi 476 220 sac sac NN cord-104239-xxlcdbqi 476 221 cells cell NNS cord-104239-xxlcdbqi 476 222 : : : cord-104239-xxlcdbqi 476 223 determination determination NN cord-104239-xxlcdbqi 476 224 of of IN cord-104239-xxlcdbqi 476 225 the the DT cord-104239-xxlcdbqi 476 226 first first JJ cord-104239-xxlcdbqi 476 227 site site NN cord-104239-xxlcdbqi 476 228 of of IN cord-104239-xxlcdbqi 476 229 budding budding NN cord-104239-xxlcdbqi 476 230 of of IN cord-104239-xxlcdbqi 476 231 progeny progeny JJ cord-104239-xxlcdbqi 476 232 virions virion NNS cord-104239-xxlcdbqi 476 233 Site site NN cord-104239-xxlcdbqi 476 234 of of IN cord-104239-xxlcdbqi 476 235 addition addition NN cord-104239-xxlcdbqi 476 236 of of IN cord-104239-xxlcdbqi 476 237 N n NN cord-104239-xxlcdbqi 476 238 - - HYPH cord-104239-xxlcdbqi 476 239 acetyl acetyl NN cord-104239-xxlcdbqi 476 240 - - HYPH cord-104239-xxlcdbqi 476 241 galactosamine galactosamine NN cord-104239-xxlcdbqi 476 242 to to IN cord-104239-xxlcdbqi 476 243 the the DT cord-104239-xxlcdbqi 476 244 E1 e1 NN cord-104239-xxlcdbqi 476 245 glycoprotein glycoprotein NN cord-104239-xxlcdbqi 476 246 of of IN cord-104239-xxlcdbqi 476 247 mouse mouse NN cord-104239-xxlcdbqi 476 248 hepatitis hepatitis NN cord-104239-xxlcdbqi 476 249 virus virus NN cord-104239-xxlcdbqi 476 250 - - : cord-104239-xxlcdbqi 476 251 A59 A59 NNP cord-104239-xxlcdbqi 476 252 Stereoiogical Stereoiogical NNP cord-104239-xxlcdbqi 476 253 Methods Methods NNPS cord-104239-xxlcdbqi 476 254 . . . cord-104239-xxlcdbqi 477 1 1 1 LS cord-104239-xxlcdbqi 477 2 . . . cord-104239-xxlcdbqi 478 1 Practical practical JJ cord-104239-xxlcdbqi 478 2 Methods Methods NNPS cord-104239-xxlcdbqi 478 3 for for IN cord-104239-xxlcdbqi 478 4 Biological Biological NNP cord-104239-xxlcdbqi 478 5 Morphometry Morphometry NNP cord-104239-xxlcdbqi 479 1 A a DT cord-104239-xxlcdbqi 479 2 principle principle NN cord-104239-xxlcdbqi 479 3 for for IN cord-104239-xxlcdbqi 479 4 counting count VBG cord-104239-xxlcdbqi 479 5 tissue tissue NN cord-104239-xxlcdbqi 479 6 structures structure NNS cord-104239-xxlcdbqi 479 7 on on IN cord-104239-xxlcdbqi 479 8 random random JJ cord-104239-xxlcdbqi 479 9 sections section NNS cord-104239-xxlcdbqi 479 10 Integrated integrate VBN cord-104239-xxlcdbqi 479 11 stereological stereological JJ cord-104239-xxlcdbqi 479 12 and and CC cord-104239-xxlcdbqi 479 13 biochemical biochemical JJ cord-104239-xxlcdbqi 479 14 studies study NNS cord-104239-xxlcdbqi 479 15 on on IN cord-104239-xxlcdbqi 479 16 hepatocytic hepatocytic JJ cord-104239-xxlcdbqi 479 17 membranes membrane NNS cord-104239-xxlcdbqi 479 18 . . . cord-104239-xxlcdbqi 480 1 II II NNP cord-104239-xxlcdbqi 480 2 . . . cord-104239-xxlcdbqi 481 1 Correction correction NN cord-104239-xxlcdbqi 481 2 of of IN cord-104239-xxlcdbqi 481 3 section section NN cord-104239-xxlcdbqi 481 4 thickness thickness NN cord-104239-xxlcdbqi 481 5 effect effect NN cord-104239-xxlcdbqi 481 6 on on IN cord-104239-xxlcdbqi 481 7 volume volume NN cord-104239-xxlcdbqi 481 8 and and CC cord-104239-xxlcdbqi 481 9 surface surface NN cord-104239-xxlcdbqi 481 10 density density NN cord-104239-xxlcdbqi 481 11 estimates estimate VBZ cord-104239-xxlcdbqi 481 12 Golgi Golgi NNP cord-104239-xxlcdbqi 481 13 membrane membrane NN cord-104239-xxlcdbqi 481 14 dynamics dynamic NNS cord-104239-xxlcdbqi 481 15 imaged image VBN cord-104239-xxlcdbqi 481 16 by by IN cord-104239-xxlcdbqi 481 17 freeze freeze NN cord-104239-xxlcdbqi 481 18 - - HYPH cord-104239-xxlcdbqi 481 19 etch etch NN cord-104239-xxlcdbqi 481 20 electron electron NN cord-104239-xxlcdbqi 481 21 microscopy microscopy NN cord-104239-xxlcdbqi 481 22 : : : cord-104239-xxlcdbqi 481 23 views view NNS cord-104239-xxlcdbqi 481 24 of of IN cord-104239-xxlcdbqi 481 25 different different JJ cord-104239-xxlcdbqi 481 26 membrane membrane NN cord-104239-xxlcdbqi 481 27 coatings coating NNS cord-104239-xxlcdbqi 481 28 involved involve VBN cord-104239-xxlcdbqi 481 29 in in IN cord-104239-xxlcdbqi 481 30 tubulation tubulation NN cord-104239-xxlcdbqi 481 31 versus versus IN cord-104239-xxlcdbqi 481 32 vesiculation vesiculation NN cord-104239-xxlcdbqi 481 33 Speculations speculation NNS cord-104239-xxlcdbqi 481 34 based base VBN cord-104239-xxlcdbqi 481 35 on on IN cord-104239-xxlcdbqi 481 36 the the DT cord-104239-xxlcdbqi 481 37 morphology morphology NN cord-104239-xxlcdbqi 481 38 of of IN cord-104239-xxlcdbqi 481 39 the the DT cord-104239-xxlcdbqi 481 40 Golgi Golgi NNP cord-104239-xxlcdbqi 481 41 system system NN cord-104239-xxlcdbqi 481 42 in in IN cord-104239-xxlcdbqi 481 43 several several JJ cord-104239-xxlcdbqi 481 44 types type NNS cord-104239-xxlcdbqi 481 45 of of IN cord-104239-xxlcdbqi 481 46 protein protein NN cord-104239-xxlcdbqi 481 47 secreting secreting NN cord-104239-xxlcdbqi 481 48 cells cell NNS cord-104239-xxlcdbqi 482 1 We -PRON- PRP cord-104239-xxlcdbqi 482 2 thank thank VBP cord-104239-xxlcdbqi 482 3 Dr. Dr. NNP cord-104239-xxlcdbqi 482 4 G. G. NNP cord-104239-xxlcdbqi 482 5 Palade Palade NNP cord-104239-xxlcdbqi 482 6 , , , cord-104239-xxlcdbqi 482 7 Dr. Dr. NNP cord-104239-xxlcdbqi 482 8 M.G. M.G. NNP cord-104239-xxlcdbqi 482 9 Farquhar Farquhar NNP cord-104239-xxlcdbqi 482 10 , , , cord-104239-xxlcdbqi 482 11 and and CC cord-104239-xxlcdbqi 482 12 Michael Michael NNP cord-104239-xxlcdbqi 482 13 McCaffery McCaffery NNP cord-104239-xxlcdbqi 482 14 for for IN cord-104239-xxlcdbqi 482 15 their -PRON- PRP$ cord-104239-xxlcdbqi 482 16 many many JJ cord-104239-xxlcdbqi 482 17 helpful helpful JJ cord-104239-xxlcdbqi 482 18 comments comment NNS cord-104239-xxlcdbqi 482 19 concerning concern VBG cord-104239-xxlcdbqi 482 20 the the DT cord-104239-xxlcdbqi 482 21 EM EM NNP cord-104239-xxlcdbqi 482 22 . . . cord-104239-xxlcdbqi 483 1 This this DT cord-104239-xxlcdbqi 483 2 work work NN cord-104239-xxlcdbqi 483 3 was be VBD cord-104239-xxlcdbqi 483 4 supported support VBN cord-104239-xxlcdbqi 483 5 by by IN cord-104239-xxlcdbqi 483 6 grants grant NNS cord-104239-xxlcdbqi 483 7 from from IN cord-104239-xxlcdbqi 483 8 the the DT cord-104239-xxlcdbqi 483 9 National National NNP cord-104239-xxlcdbqi 483 10 Institutes Institutes NNPS cord-104239-xxlcdbqi 483 11 of of IN cord-104239-xxlcdbqi 483 12 Health Health NNP cord-104239-xxlcdbqi 483 13 ( ( -LRB- cord-104239-xxlcdbqi 483 14 GM GM NNP cord-104239-xxlcdbqi 483 15 42336 42336 CD cord-104239-xxlcdbqi 483 16 ; ; : cord-104239-xxlcdbqi 483 17 CA CA NNP cord-104239-xxlcdbqi 483 18 586689 586689 CD cord-104239-xxlcdbqi 483 19 ) ) -RRB- cord-104239-xxlcdbqi 483 20 ( ( -LRB- cord-104239-xxlcdbqi 483 21 to to IN cord-104239-xxlcdbqi 483 22 W.E. W.E. NNP cord-104239-xxlcdbqi 483 23 Balch Balch NNP cord-104239-xxlcdbqi 483 24 ) ) -RRB- cord-104239-xxlcdbqi 483 25 , , , cord-104239-xxlcdbqi 483 26 and and CC cord-104239-xxlcdbqi 483 27 postdoctoral postdoctoral JJ cord-104239-xxlcdbqi 483 28 fellowships fellowship NNS cord-104239-xxlcdbqi 483 29 from from IN cord-104239-xxlcdbqi 483 30 The the DT cord-104239-xxlcdbqi 483 31 Human Human NNP cord-104239-xxlcdbqi 483 32 Frontier Frontier NNP cord-104239-xxlcdbqi 483 33 Science Science NNP cord-104239-xxlcdbqi 483 34 Program Program NNP cord-104239-xxlcdbqi 483 35 Organization Organization NNP cord-104239-xxlcdbqi 483 36 , , , cord-104239-xxlcdbqi 483 37 Muscular Muscular NNP cord-104239-xxlcdbqi 483 38 Dystrophy Dystrophy NNP cord-104239-xxlcdbqi 483 39 Association Association NNP cord-104239-xxlcdbqi 483 40 ( ( -LRB- cord-104239-xxlcdbqi 483 41 to to IN cord-104239-xxlcdbqi 483 42 T. T. NNP cord-104239-xxlcdbqi 483 43 Rowe Rowe NNP cord-104239-xxlcdbqi 483 44 ) ) -RRB- cord-104239-xxlcdbqi 483 45 , , , cord-104239-xxlcdbqi 483 46 and and CC cord-104239-xxlcdbqi 483 47 the the DT cord-104239-xxlcdbqi 483 48 Cystic Cystic NNP cord-104239-xxlcdbqi 483 49 Fibrosis Fibrosis NNP cord-104239-xxlcdbqi 483 50 Foundation Foundation NNP cord-104239-xxlcdbqi 483 51 ( ( -LRB- cord-104239-xxlcdbqi 483 52 to to IN cord-104239-xxlcdbqi 483 53 S. S. NNP cord-104239-xxlcdbqi 483 54 Bannykh Bannykh NNP cord-104239-xxlcdbqi 483 55 ) ) -RRB- cord-104239-xxlcdbqi 483 56 . . . cord-104239-xxlcdbqi 484 1 This this DT cord-104239-xxlcdbqi 484 2 study study NN cord-104239-xxlcdbqi 484 3 made make VBD cord-104239-xxlcdbqi 484 4 extensive extensive JJ cord-104239-xxlcdbqi 484 5 use use NN cord-104239-xxlcdbqi 484 6 of of IN cord-104239-xxlcdbqi 484 7 Core core NN cord-104239-xxlcdbqi 484 8 B B NNP cord-104239-xxlcdbqi 484 9 ( ( -LRB- cord-104239-xxlcdbqi 484 10 Immunoelectron Immunoelectron NNP cord-104239-xxlcdbqi 484 11 Microscopy Microscopy NNP cord-104239-xxlcdbqi 484 12 ) ) -RRB- cord-104239-xxlcdbqi 484 13 in in IN cord-104239-xxlcdbqi 484 14 CA CA NNP cord-104239-xxlcdbqi 484 15 586689.Received 586689.received CD cord-104239-xxlcdbqi 484 16 for for IN cord-104239-xxlcdbqi 484 17 publication publication NN cord-104239-xxlcdbqi 484 18 22 22 CD cord-104239-xxlcdbqi 484 19 April April NNP cord-104239-xxlcdbqi 484 20 1996 1996 CD cord-104239-xxlcdbqi 484 21 and and CC cord-104239-xxlcdbqi 484 22 in in IN cord-104239-xxlcdbqi 484 23 revised revise VBN cord-104239-xxlcdbqi 484 24 form form NN cord-104239-xxlcdbqi 484 25 11 11 CD cord-104239-xxlcdbqi 484 26 June June NNP cord-104239-xxlcdbqi 484 27 1996 1996 CD cord-104239-xxlcdbqi 484 28 . . . cord-104239-xxlcdbqi 485 1 A a DT cord-104239-xxlcdbqi 485 2 third third JJ cord-104239-xxlcdbqi 485 3 tier tier NN cord-104239-xxlcdbqi 485 4 of of IN cord-104239-xxlcdbqi 485 5 organization organization NN cord-104239-xxlcdbqi 485 6 of of IN cord-104239-xxlcdbqi 485 7 export export NN cord-104239-xxlcdbqi 485 8 sites site NNS cord-104239-xxlcdbqi 485 9 was be VBD cord-104239-xxlcdbqi 485 10 found find VBN cord-104239-xxlcdbqi 485 11 in in IN cord-104239-xxlcdbqi 485 12 the the DT cord-104239-xxlcdbqi 485 13 striking striking JJ cord-104239-xxlcdbqi 485 14 relationship relationship NN cord-104239-xxlcdbqi 485 15 between between IN cord-104239-xxlcdbqi 485 16 flanking flank VBG cord-104239-xxlcdbqi 485 17 ER ER NNP cord-104239-xxlcdbqi 485 18 - - HYPH cord-104239-xxlcdbqi 485 19 connected connect VBN cord-104239-xxlcdbqi 485 20 budding budding JJ cord-104239-xxlcdbqi 485 21 profiles profile NNS cord-104239-xxlcdbqi 485 22 and and CC cord-104239-xxlcdbqi 485 23 VTCs vtc NNS cord-104239-xxlcdbqi 485 24 to to TO cord-104239-xxlcdbqi 485 25 form form VB cord-104239-xxlcdbqi 485 26 the the DT cord-104239-xxlcdbqi 485 27 functional functional JJ cord-104239-xxlcdbqi 485 28 morphological morphological JJ cord-104239-xxlcdbqi 485 29 unit unit NN cord-104239-xxlcdbqi 485 30 we -PRON- PRP cord-104239-xxlcdbqi 485 31 refer refer VBP cord-104239-xxlcdbqi 485 32 to to IN cord-104239-xxlcdbqi 485 33 as as IN cord-104239-xxlcdbqi 485 34 export export NN cord-104239-xxlcdbqi 485 35 complexes complex NNS cord-104239-xxlcdbqi 485 36 ( ( -LRB- cord-104239-xxlcdbqi 485 37 Fig Fig NNP cord-104239-xxlcdbqi 485 38 . . . cord-104239-xxlcdbqi 485 39 11 11 CD cord-104239-xxlcdbqi 485 40 A A NNP cord-104239-xxlcdbqi 485 41 , , , cord-104239-xxlcdbqi 485 42 area area NN cord-104239-xxlcdbqi 485 43 enclosed enclose VBN cord-104239-xxlcdbqi 485 44 by by IN cord-104239-xxlcdbqi 485 45 box box NN cord-104239-xxlcdbqi 485 46 with with IN cord-104239-xxlcdbqi 485 47 solid solid JJ cord-104239-xxlcdbqi 485 48 line line NN cord-104239-xxlcdbqi 485 49 ) ) -RRB- cord-104239-xxlcdbqi 485 50 . . . cord-104239-xxlcdbqi 486 1 Images image NNS cord-104239-xxlcdbqi 486 2 reconstructed reconstruct VBN cord-104239-xxlcdbqi 486 3 from from IN cord-104239-xxlcdbqi 486 4 conventional conventional JJ cord-104239-xxlcdbqi 486 5 TEM tem NN cord-104239-xxlcdbqi 486 6 and and CC cord-104239-xxlcdbqi 486 7 those those DT cord-104239-xxlcdbqi 486 8 observed observe VBN cord-104239-xxlcdbqi 486 9 in in IN cord-104239-xxlcdbqi 486 10 replicas replica NNS cord-104239-xxlcdbqi 486 11 yielded yield VBD cord-104239-xxlcdbqi 486 12 identical identical JJ cord-104239-xxlcdbqi 486 13 results result NNS cord-104239-xxlcdbqi 486 14 . . . cord-104239-xxlcdbqi 487 1 The the DT cord-104239-xxlcdbqi 487 2 distinctive distinctive JJ cord-104239-xxlcdbqi 487 3 morphological morphological JJ cord-104239-xxlcdbqi 487 4 characteristics characteristic NNS cord-104239-xxlcdbqi 487 5 of of IN cord-104239-xxlcdbqi 487 6 the the DT cord-104239-xxlcdbqi 487 7 export export NN cord-104239-xxlcdbqi 487 8 complexes complex NNS cord-104239-xxlcdbqi 487 9 reconstructed reconstruct VBN cord-104239-xxlcdbqi 487 10 in in IN cord-104239-xxlcdbqi 487 11 the the DT cord-104239-xxlcdbqi 487 12 present present JJ cord-104239-xxlcdbqi 487 13 studies study NNS cord-104239-xxlcdbqi 487 14 are be VBP cord-104239-xxlcdbqi 487 15 consistent consistent JJ cord-104239-xxlcdbqi 487 16 with with IN cord-104239-xxlcdbqi 487 17 previous previous JJ cord-104239-xxlcdbqi 487 18 qualitative qualitative JJ cord-104239-xxlcdbqi 487 19 morphological morphological JJ cord-104239-xxlcdbqi 487 20 descriptions description NNS cord-104239-xxlcdbqi 487 21 ( ( -LRB- cord-104239-xxlcdbqi 487 22 Saraste Saraste NNP cord-104239-xxlcdbqi 487 23 and and CC cord-104239-xxlcdbqi 487 24 Kuismanen Kuismanen NNP cord-104239-xxlcdbqi 487 25 , , , cord-104239-xxlcdbqi 487 26 1984 1984 CD cord-104239-xxlcdbqi 487 27 ; ; : cord-104239-xxlcdbqi 487 28 Schweizer Schweizer NNP cord-104239-xxlcdbqi 487 29 et et NNP cord-104239-xxlcdbqi 487 30 al al NNP cord-104239-xxlcdbqi 487 31 . . NNP cord-104239-xxlcdbqi 487 32 , , , cord-104239-xxlcdbqi 487 33 1988 1988 CD cord-104239-xxlcdbqi 487 34 Schweizer Schweizer NNP cord-104239-xxlcdbqi 487 35 et et NNP cord-104239-xxlcdbqi 487 36 al al NNP cord-104239-xxlcdbqi 487 37 . . . cord-104239-xxlcdbqi 488 1 , , , cord-104239-xxlcdbqi 488 2 , , , cord-104239-xxlcdbqi 488 3 1990 1990 CD cord-104239-xxlcdbqi 488 4 Saraste Saraste NNP cord-104239-xxlcdbqi 488 5 and and CC cord-104239-xxlcdbqi 488 6 Svensson Svensson NNP cord-104239-xxlcdbqi 488 7 , , , cord-104239-xxlcdbqi 488 8 1991 1991 CD cord-104239-xxlcdbqi 488 9 ) ) -RRB- cord-104239-xxlcdbqi 488 10 and and CC cord-104239-xxlcdbqi 488 11 a a DT cord-104239-xxlcdbqi 488 12 recent recent JJ cord-104239-xxlcdbqi 488 13 study study NN cord-104239-xxlcdbqi 488 14 in in IN cord-104239-xxlcdbqi 488 15 which which WDT cord-104239-xxlcdbqi 488 16 a a DT cord-104239-xxlcdbqi 488 17 HRP HRP NNP cord-104239-xxlcdbqi 488 18 - - HYPH cord-104239-xxlcdbqi 488 19 tagged tag VBN cord-104239-xxlcdbqi 488 20 reporter reporter NN cord-104239-xxlcdbqi 488 21 protein protein NN cord-104239-xxlcdbqi 488 22 was be VBD cord-104239-xxlcdbqi 488 23 used use VBN cord-104239-xxlcdbqi 488 24 to to TO cord-104239-xxlcdbqi 488 25 characterize characterize VB cord-104239-xxlcdbqi 488 26 the the DT cord-104239-xxlcdbqi 488 27 organization organization NN cord-104239-xxlcdbqi 488 28 of of IN cord-104239-xxlcdbqi 488 29 the the DT cord-104239-xxlcdbqi 488 30 ER ER NNP cord-104239-xxlcdbqi 488 31 / / SYM cord-104239-xxlcdbqi 488 32 Golgi Golgi NNP cord-104239-xxlcdbqi 488 33 region region NN cord-104239-xxlcdbqi 488 34 using use VBG cord-104239-xxlcdbqi 488 35 immunocytochemistry immunocytochemistry NNP cord-104239-xxlcdbqi 489 1 ( ( -LRB- cord-104239-xxlcdbqi 489 2 Stinchcombe Stinchcombe NNP cord-104239-xxlcdbqi 489 3 et et NNP cord-104239-xxlcdbqi 489 4 al al NNP cord-104239-xxlcdbqi 489 5 . . NNP cord-104239-xxlcdbqi 489 6 , , , cord-104239-xxlcdbqi 489 7 1995 1995 CD cord-104239-xxlcdbqi 489 8 ) ) -RRB- cord-104239-xxlcdbqi 489 9 . . . cord-104239-xxlcdbqi 490 1 The the DT cord-104239-xxlcdbqi 490 2 replicas replica NNS cord-104239-xxlcdbqi 490 3 were be VBD cord-104239-xxlcdbqi 490 4 particularly particularly RB cord-104239-xxlcdbqi 490 5 striking strike VBG cord-104239-xxlcdbqi 490 6 in in IN cord-104239-xxlcdbqi 490 7 that that IN cord-104239-xxlcdbqi 490 8 they -PRON- PRP cord-104239-xxlcdbqi 490 9 allowed allow VBD cord-104239-xxlcdbqi 490 10 us -PRON- PRP cord-104239-xxlcdbqi 490 11 to to TO cord-104239-xxlcdbqi 490 12 visualize visualize VB cord-104239-xxlcdbqi 490 13 for for IN cord-104239-xxlcdbqi 490 14 first first JJ cord-104239-xxlcdbqi 490 15 time time NN cord-104239-xxlcdbqi 490 16 the the DT cord-104239-xxlcdbqi 490 17 overall overall JJ cord-104239-xxlcdbqi 490 18 compact compact JJ cord-104239-xxlcdbqi 490 19 composition composition NN cord-104239-xxlcdbqi 490 20 of of IN cord-104239-xxlcdbqi 490 21 VTCs vtc NNS cord-104239-xxlcdbqi 490 22 and and CC cord-104239-xxlcdbqi 490 23 their -PRON- PRP$ cord-104239-xxlcdbqi 490 24 localization localization NN cord-104239-xxlcdbqi 490 25 to to IN cord-104239-xxlcdbqi 490 26 specific specific JJ cord-104239-xxlcdbqi 490 27 foci focus NNS cord-104239-xxlcdbqi 490 28 found find VBN cord-104239-xxlcdbqi 490 29 on on IN cord-104239-xxlcdbqi 490 30 the the DT cord-104239-xxlcdbqi 490 31 ER ER NNP cord-104239-xxlcdbqi 490 32 surface surface NN cord-104239-xxlcdbqi 490 33 . . . cord-104239-xxlcdbqi 491 1 The the DT cord-104239-xxlcdbqi 491 2 overall overall JJ cord-104239-xxlcdbqi 491 3 topological topological JJ cord-104239-xxlcdbqi 491 4 organization organization NN cord-104239-xxlcdbqi 491 5 of of IN cord-104239-xxlcdbqi 491 6 export export NN cord-104239-xxlcdbqi 491 7 complexes complex NNS cord-104239-xxlcdbqi 491 8 fits fit VBZ cord-104239-xxlcdbqi 491 9 well well RB cord-104239-xxlcdbqi 491 10 with with IN cord-104239-xxlcdbqi 491 11 the the DT cord-104239-xxlcdbqi 491 12 proposed propose VBN cord-104239-xxlcdbqi 491 13 function function NN cord-104239-xxlcdbqi 491 14 of of IN cord-104239-xxlcdbqi 491 15 the the DT cord-104239-xxlcdbqi 491 16 ER ER NNP cord-104239-xxlcdbqi 491 17 in in IN cord-104239-xxlcdbqi 491 18 the the DT cord-104239-xxlcdbqi 491 19 sorting sorting NN cord-104239-xxlcdbqi 491 20 and and CC cord-104239-xxlcdbqi 491 21 concentration concentration NN cord-104239-xxlcdbqi 491 22 of of IN cord-104239-xxlcdbqi 491 23 cargo cargo NN cord-104239-xxlcdbqi 491 24 during during IN cord-104239-xxlcdbqi 491 25 budding bud VBG cord-104239-xxlcdbqi 491 26 via via IN cord-104239-xxlcdbqi 491 27 COPII COPII NNP cord-104239-xxlcdbqi 491 28 coats coat NNS cord-104239-xxlcdbqi 491 29 , , , cord-104239-xxlcdbqi 491 30 and and CC cord-104239-xxlcdbqi 491 31 the the DT cord-104239-xxlcdbqi 491 32 subsequent subsequent JJ cord-104239-xxlcdbqi 491 33 coupled coupled JJ cord-104239-xxlcdbqi 491 34 recycling recycling NN cord-104239-xxlcdbqi 491 35 of of IN cord-104239-xxlcdbqi 491 36 proteins protein NNS cord-104239-xxlcdbqi 491 37 from from IN cord-104239-xxlcdbqi 491 38 VTCs vtc NNS cord-104239-xxlcdbqi 491 39 via via IN cord-104239-xxlcdbqi 491 40 COPI COPI NNP cord-104239-xxlcdbqi 491 41 coats coat NNS cord-104239-xxlcdbqi 492 1 ( ( -LRB- cord-104239-xxlcdbqi 492 2 Aridor Aridor NNP cord-104239-xxlcdbqi 492 3 et et NNP cord-104239-xxlcdbqi 492 4 al al NNP cord-104239-xxlcdbqi 492 5 . . NNP cord-104239-xxlcdbqi 492 6 , , , cord-104239-xxlcdbqi 492 7 1995 1995 CD cord-104239-xxlcdbqi 492 8 ) ) -RRB- cord-104239-xxlcdbqi 492 9 . . . cord-104239-xxlcdbqi 493 1 The the DT cord-104239-xxlcdbqi 493 2 close close JJ cord-104239-xxlcdbqi 493 3 association association NN cord-104239-xxlcdbqi 493 4 of of IN cord-104239-xxlcdbqi 493 5 these these DT cord-104239-xxlcdbqi 493 6 two two CD cord-104239-xxlcdbqi 493 7 sorting sorting NN cord-104239-xxlcdbqi 493 8 stations station NNS cord-104239-xxlcdbqi 493 9 ( ( -LRB- cord-104239-xxlcdbqi 493 10 ER ER NNP cord-104239-xxlcdbqi 493 11 and and CC cord-104239-xxlcdbqi 493 12 VTCs VTCs NNP cord-104239-xxlcdbqi 493 13 ) ) -RRB- cord-104239-xxlcdbqi 493 14 may may MD cord-104239-xxlcdbqi 493 15 provide provide VB cord-104239-xxlcdbqi 493 16 for for IN cord-104239-xxlcdbqi 493 17 increased increase VBN cord-104239-xxlcdbqi 493 18 efficiency efficiency NN cord-104239-xxlcdbqi 493 19 in in IN cord-104239-xxlcdbqi 493 20 ER ER NNP cord-104239-xxlcdbqi 493 21 to to IN cord-104239-xxlcdbqi 493 22 Golgi Golgi NNP cord-104239-xxlcdbqi 493 23 transport transport NN cord-104239-xxlcdbqi 493 24 and/or and/or CC cord-104239-xxlcdbqi 493 25 promote promote VB cord-104239-xxlcdbqi 493 26 more more RBR cord-104239-xxlcdbqi 493 27 rapid rapid JJ cord-104239-xxlcdbqi 493 28 exchange exchange NN cord-104239-xxlcdbqi 493 29 of of IN cord-104239-xxlcdbqi 493 30 coats coat NNS cord-104239-xxlcdbqi 493 31 within within IN cord-104239-xxlcdbqi 493 32 the the DT cord-104239-xxlcdbqi 493 33 confines confine NNS cord-104239-xxlcdbqi 493 34 of of IN cord-104239-xxlcdbqi 493 35 the the DT cord-104239-xxlcdbqi 493 36 complex complex NN cord-104239-xxlcdbqi 493 37 where where WRB cord-104239-xxlcdbqi 493 38 a a DT cord-104239-xxlcdbqi 493 39 higher high JJR cord-104239-xxlcdbqi 493 40 local local JJ cord-104239-xxlcdbqi 493 41 coat coat NN cord-104239-xxlcdbqi 493 42 concentration concentration NN cord-104239-xxlcdbqi 493 43 may may MD cord-104239-xxlcdbqi 493 44 be be VB cord-104239-xxlcdbqi 493 45 found find VBN cord-104239-xxlcdbqi 493 46 . . . cord-104239-xxlcdbqi 494 1 Consistent consistent JJ cord-104239-xxlcdbqi 494 2 with with IN cord-104239-xxlcdbqi 494 3 this this DT cord-104239-xxlcdbqi 494 4 proposal proposal NN cord-104239-xxlcdbqi 494 5 , , , cord-104239-xxlcdbqi 494 6 incubation incubation NN cord-104239-xxlcdbqi 494 7 of of IN cord-104239-xxlcdbqi 494 8 pancreatic pancreatic JJ cord-104239-xxlcdbqi 494 9 acinar acinar NN cord-104239-xxlcdbqi 494 10 cells cell NNS cord-104239-xxlcdbqi 494 11 in in IN cord-104239-xxlcdbqi 494 12 the the DT cord-104239-xxlcdbqi 494 13 absence absence NN cord-104239-xxlcdbqi 494 14 of of IN cord-104239-xxlcdbqi 494 15 ATP ATP NNP cord-104239-xxlcdbqi 494 16 ( ( -LRB- cord-104239-xxlcdbqi 494 17 Merisko Merisko NNP cord-104239-xxlcdbqi 494 18 et et NNP cord-104239-xxlcdbqi 494 19 al al NNP cord-104239-xxlcdbqi 494 20 . . NNP cord-104239-xxlcdbqi 494 21 , , , cord-104239-xxlcdbqi 494 22 1986 1986 CD cord-104239-xxlcdbqi 494 23 ) ) -RRB- cord-104239-xxlcdbqi 494 24 , , , cord-104239-xxlcdbqi 494 25 1992 1992 CD cord-104239-xxlcdbqi 494 26 ) ) -RRB- cord-104239-xxlcdbqi 494 27 , , , cord-104239-xxlcdbqi 494 28 is be VBZ cord-104239-xxlcdbqi 494 29 also also RB cord-104239-xxlcdbqi 494 30 considered consider VBN cord-104239-xxlcdbqi 494 31 to to TO cord-104239-xxlcdbqi 494 32 be be VB cord-104239-xxlcdbqi 494 33 a a DT cord-104239-xxlcdbqi 494 34 site site NN cord-104239-xxlcdbqi 494 35 of of IN cord-104239-xxlcdbqi 494 36 membrane membrane NN cord-104239-xxlcdbqi 494 37 recycling recycling NN cord-104239-xxlcdbqi 494 38 . . . cord-104239-xxlcdbqi 495 1 It -PRON- PRP cord-104239-xxlcdbqi 495 2 may may MD cord-104239-xxlcdbqi 495 3 be be VB cord-104239-xxlcdbqi 495 4 an an DT cord-104239-xxlcdbqi 495 5 enlarged enlarged JJ cord-104239-xxlcdbqi 495 6 variation variation NN cord-104239-xxlcdbqi 495 7 of of IN cord-104239-xxlcdbqi 495 8 the the DT cord-104239-xxlcdbqi 495 9 more more RBR cord-104239-xxlcdbqi 495 10 compact compact JJ cord-104239-xxlcdbqi 495 11 VTCs vtc NNS cord-104239-xxlcdbqi 495 12 , , , cord-104239-xxlcdbqi 495 13 reflecting reflect VBG cord-104239-xxlcdbqi 495 14 the the DT cord-104239-xxlcdbqi 495 15 intensity intensity NN cord-104239-xxlcdbqi 495 16 of of IN cord-104239-xxlcdbqi 495 17 vesicular vesicular JJ cord-104239-xxlcdbqi 495 18 traffic traffic NN cord-104239-xxlcdbqi 495 19 in in IN cord-104239-xxlcdbqi 495 20 this this DT cord-104239-xxlcdbqi 495 21 region region NN cord-104239-xxlcdbqi 495 22 of of IN cord-104239-xxlcdbqi 495 23 the the DT cord-104239-xxlcdbqi 495 24 cell cell NN cord-104239-xxlcdbqi 495 25 . . . cord-104239-xxlcdbqi 496 1 In in IN cord-104239-xxlcdbqi 496 2 general general JJ cord-104239-xxlcdbqi 496 3 , , , cord-104239-xxlcdbqi 496 4 our -PRON- PRP$ cord-104239-xxlcdbqi 496 5 studies study NNS cord-104239-xxlcdbqi 496 6 have have VBP cord-104239-xxlcdbqi 496 7 provided provide VBN cord-104239-xxlcdbqi 496 8 insight insight NN cord-104239-xxlcdbqi 496 9 into into IN cord-104239-xxlcdbqi 496 10 the the DT cord-104239-xxlcdbqi 496 11 fundamental fundamental JJ cord-104239-xxlcdbqi 496 12 morphological morphological JJ cord-104239-xxlcdbqi 496 13 organization organization NN cord-104239-xxlcdbqi 496 14 of of IN cord-104239-xxlcdbqi 496 15 the the DT cord-104239-xxlcdbqi 496 16 first first JJ cord-104239-xxlcdbqi 496 17 steps step NNS cord-104239-xxlcdbqi 496 18 in in IN cord-104239-xxlcdbqi 496 19 the the DT cord-104239-xxlcdbqi 496 20 secretory secretory JJ cord-104239-xxlcdbqi 496 21 pathway pathway NN cord-104239-xxlcdbqi 496 22 that that WDT cord-104239-xxlcdbqi 496 23 promote promote VBP cord-104239-xxlcdbqi 496 24 the the DT cord-104239-xxlcdbqi 496 25 movement movement NN cord-104239-xxlcdbqi 496 26 of of IN cord-104239-xxlcdbqi 496 27 cargo cargo NN cord-104239-xxlcdbqi 496 28 from from IN cord-104239-xxlcdbqi 496 29 the the DT cord-104239-xxlcdbqi 496 30 ER ER NNP cord-104239-xxlcdbqi 496 31 to to IN cord-104239-xxlcdbqi 496 32 the the DT cord-104239-xxlcdbqi 496 33 Golgi Golgi NNP cord-104239-xxlcdbqi 496 34 complex complex NN cord-104239-xxlcdbqi 496 35 . . . cord-104239-xxlcdbqi 497 1 There there EX cord-104239-xxlcdbqi 497 2 has have VBZ cord-104239-xxlcdbqi 497 3 been be VBN cord-104239-xxlcdbqi 497 4 considerable considerable JJ cord-104239-xxlcdbqi 497 5 controversy controversy NN cord-104239-xxlcdbqi 497 6 regarding regard VBG cord-104239-xxlcdbqi 497 7 the the DT cord-104239-xxlcdbqi 497 8 morphological morphological JJ cord-104239-xxlcdbqi 497 9 organization organization NN cord-104239-xxlcdbqi 497 10 of of IN cord-104239-xxlcdbqi 497 11 this this DT cord-104239-xxlcdbqi 497 12 stage stage NN cord-104239-xxlcdbqi 497 13 of of IN cord-104239-xxlcdbqi 497 14 secretory secretory JJ cord-104239-xxlcdbqi 497 15 pathway pathway NN cord-104239-xxlcdbqi 497 16 , , , cord-104239-xxlcdbqi 497 17 given give VBN cord-104239-xxlcdbqi 497 18 the the DT cord-104239-xxlcdbqi 497 19 complexity complexity NN cord-104239-xxlcdbqi 497 20 of of IN cord-104239-xxlcdbqi 497 21 the the DT cord-104239-xxlcdbqi 497 22 pre pre JJ cord-104239-xxlcdbqi 497 23 - - JJ cord-104239-xxlcdbqi 497 24 Golgi Golgi NNP cord-104239-xxlcdbqi 497 25 region region NN cord-104239-xxlcdbqi 497 26 and and CC cord-104239-xxlcdbqi 497 27 the the DT cord-104239-xxlcdbqi 497 28 frequent frequent JJ cord-104239-xxlcdbqi 497 29 use use NN cord-104239-xxlcdbqi 497 30 of of IN cord-104239-xxlcdbqi 497 31 reduced reduce VBN cord-104239-xxlcdbqi 497 32 temperature temperature NN cord-104239-xxlcdbqi 497 33 to to TO cord-104239-xxlcdbqi 497 34 augment augment VB cord-104239-xxlcdbqi 497 35 the the DT cord-104239-xxlcdbqi 497 36 visibility visibility NN cord-104239-xxlcdbqi 497 37 of of IN cord-104239-xxlcdbqi 497 38 intermediates intermediate NNS cord-104239-xxlcdbqi 497 39 ( ( -LRB- cord-104239-xxlcdbqi 497 40 VTCs VTCs NNP cord-104239-xxlcdbqi 497 41 ) ) -RRB- cord-104239-xxlcdbqi 497 42 . . . cord-104239-xxlcdbqi 498 1 Our -PRON- PRP$ cord-104239-xxlcdbqi 498 2 ability ability NN cord-104239-xxlcdbqi 498 3 to to TO cord-104239-xxlcdbqi 498 4 provide provide VB cord-104239-xxlcdbqi 498 5 a a DT cord-104239-xxlcdbqi 498 6 description description NN cord-104239-xxlcdbqi 498 7 of of IN cord-104239-xxlcdbqi 498 8 the the DT cord-104239-xxlcdbqi 498 9 three three CD cord-104239-xxlcdbqi 498 10 - - HYPH cord-104239-xxlcdbqi 498 11 dimensional dimensional JJ cord-104239-xxlcdbqi 498 12 organization organization NN cord-104239-xxlcdbqi 498 13 of of IN cord-104239-xxlcdbqi 498 14 export export NN cord-104239-xxlcdbqi 498 15 complexes complex NNS cord-104239-xxlcdbqi 498 16 at at IN cord-104239-xxlcdbqi 498 17 sites site NNS cord-104239-xxlcdbqi 498 18 distant distant JJ cord-104239-xxlcdbqi 498 19 from from IN cord-104239-xxlcdbqi 498 20 the the DT cord-104239-xxlcdbqi 498 21 Golgi Golgi NNP cord-104239-xxlcdbqi 498 22 apparatus apparatus NN cord-104239-xxlcdbqi 498 23 under under IN cord-104239-xxlcdbqi 498 24 normal normal JJ cord-104239-xxlcdbqi 498 25 incubation incubation NN cord-104239-xxlcdbqi 498 26 conditions condition NNS cord-104239-xxlcdbqi 498 27 now now RB cord-104239-xxlcdbqi 498 28 illustrates illustrate VBZ cord-104239-xxlcdbqi 498 29 their -PRON- PRP$ cord-104239-xxlcdbqi 498 30 basic basic JJ cord-104239-xxlcdbqi 498 31 organization organization NN cord-104239-xxlcdbqi 498 32 in in IN cord-104239-xxlcdbqi 498 33 living living NN cord-104239-xxlcdbqi 498 34 cells cell NNS cord-104239-xxlcdbqi 498 35 and and CC cord-104239-xxlcdbqi 498 36 their -PRON- PRP$ cord-104239-xxlcdbqi 498 37 essential essential JJ cord-104239-xxlcdbqi 498 38 role role NN cord-104239-xxlcdbqi 498 39 in in IN cord-104239-xxlcdbqi 498 40 ER ER NNP cord-104239-xxlcdbqi 498 41 to to IN cord-104239-xxlcdbqi 498 42 Golgi Golgi NNP cord-104239-xxlcdbqi 498 43 transport transport NN cord-104239-xxlcdbqi 498 44 . . .