id sid tid token lemma pos cord-320015-lbr2q4qh 1 1 key key NN cord-320015-lbr2q4qh 1 2 : : : cord-320015-lbr2q4qh 1 3 cord-320015-lbr2q4qh cord-320015-lbr2q4qh ADD cord-320015-lbr2q4qh 1 4 authors author NNS cord-320015-lbr2q4qh 1 5 : : : cord-320015-lbr2q4qh 1 6 Chinchar Chinchar NNP cord-320015-lbr2q4qh 1 7 , , , cord-320015-lbr2q4qh 1 8 V. V. NNP cord-320015-lbr2q4qh 1 9 Gregory Gregory NNP cord-320015-lbr2q4qh 1 10 ; ; : cord-320015-lbr2q4qh 1 11 Yu Yu NNP cord-320015-lbr2q4qh 1 12 , , , cord-320015-lbr2q4qh 1 13 Kwang Kwang NNP cord-320015-lbr2q4qh 1 14 H. H. NNP cord-320015-lbr2q4qh 1 15 ; ; : cord-320015-lbr2q4qh 1 16 Jancovich Jancovich NNP cord-320015-lbr2q4qh 1 17 , , , cord-320015-lbr2q4qh 1 18 James James NNP cord-320015-lbr2q4qh 1 19 K. K. NNP cord-320015-lbr2q4qh 1 20 title title NN cord-320015-lbr2q4qh 1 21 : : : cord-320015-lbr2q4qh 1 22 The the DT cord-320015-lbr2q4qh 1 23 Molecular Molecular NNP cord-320015-lbr2q4qh 1 24 Biology Biology NNP cord-320015-lbr2q4qh 1 25 of of IN cord-320015-lbr2q4qh 1 26 Frog Frog NNP cord-320015-lbr2q4qh 1 27 Virus Virus NNP cord-320015-lbr2q4qh 1 28 3 3 CD cord-320015-lbr2q4qh 1 29 and and CC cord-320015-lbr2q4qh 1 30 other other JJ cord-320015-lbr2q4qh 1 31 Iridoviruses Iridoviruses NNPS cord-320015-lbr2q4qh 2 1 Infecting infect VBG cord-320015-lbr2q4qh 2 2 Cold cold JJ cord-320015-lbr2q4qh 2 3 - - HYPH cord-320015-lbr2q4qh 2 4 Blooded blooded JJ cord-320015-lbr2q4qh 2 5 Vertebrates Vertebrates NNPS cord-320015-lbr2q4qh 2 6 date date NN cord-320015-lbr2q4qh 3 1 : : : cord-320015-lbr2q4qh 3 2 2011 2011 CD cord-320015-lbr2q4qh 3 3 - - SYM cord-320015-lbr2q4qh 3 4 10 10 CD cord-320015-lbr2q4qh 3 5 - - SYM cord-320015-lbr2q4qh 3 6 20 20 CD cord-320015-lbr2q4qh 3 7 journal journal NN cord-320015-lbr2q4qh 3 8 : : : cord-320015-lbr2q4qh 4 1 Viruses virus NNS cord-320015-lbr2q4qh 4 2 DOI DOI NNP cord-320015-lbr2q4qh 4 3 : : : cord-320015-lbr2q4qh 5 1 10.3390/ 10.3390/ CD cord-320015-lbr2q4qh 6 1 v3101959 v3101959 NNP cord-320015-lbr2q4qh 6 2 sha sha NNP cord-320015-lbr2q4qh 6 3 : : : cord-320015-lbr2q4qh 7 1 93bc12e23681301901cc72598ded5258b4455eb1 93bc12e23681301901cc72598ded5258b4455eb1 CD cord-320015-lbr2q4qh 7 2 doc_id doc_id CD cord-320015-lbr2q4qh 7 3 : : : cord-320015-lbr2q4qh 7 4 320015 320015 CD cord-320015-lbr2q4qh 7 5 cord_uid cord_uid NNS cord-320015-lbr2q4qh 7 6 : : : cord-320015-lbr2q4qh 7 7 lbr2q4qh lbr2q4qh ADD cord-320015-lbr2q4qh 7 8 Frog Frog NNP cord-320015-lbr2q4qh 7 9 virus virus NN cord-320015-lbr2q4qh 7 10 3 3 CD cord-320015-lbr2q4qh 8 1 ( ( -LRB- cord-320015-lbr2q4qh 8 2 FV3 FV3 NNP cord-320015-lbr2q4qh 8 3 ) ) -RRB- cord-320015-lbr2q4qh 8 4 is be VBZ cord-320015-lbr2q4qh 8 5 the the DT cord-320015-lbr2q4qh 8 6 best well RBS cord-320015-lbr2q4qh 8 7 characterized characterize VBN cord-320015-lbr2q4qh 8 8 member member NN cord-320015-lbr2q4qh 8 9 of of IN cord-320015-lbr2q4qh 8 10 the the DT cord-320015-lbr2q4qh 8 11 family family NN cord-320015-lbr2q4qh 8 12 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 8 13 . . . cord-320015-lbr2q4qh 9 1 FV3 FV3 NNP cord-320015-lbr2q4qh 9 2 study study NN cord-320015-lbr2q4qh 9 3 has have VBZ cord-320015-lbr2q4qh 9 4 provided provide VBN cord-320015-lbr2q4qh 9 5 insights insight NNS cord-320015-lbr2q4qh 9 6 into into IN cord-320015-lbr2q4qh 9 7 the the DT cord-320015-lbr2q4qh 9 8 replication replication NN cord-320015-lbr2q4qh 9 9 of of IN cord-320015-lbr2q4qh 9 10 other other JJ cord-320015-lbr2q4qh 9 11 family family NN cord-320015-lbr2q4qh 9 12 members member NNS cord-320015-lbr2q4qh 9 13 , , , cord-320015-lbr2q4qh 9 14 and and CC cord-320015-lbr2q4qh 9 15 has have VBZ cord-320015-lbr2q4qh 9 16 served serve VBN cord-320015-lbr2q4qh 9 17 as as IN cord-320015-lbr2q4qh 9 18 a a DT cord-320015-lbr2q4qh 9 19 model model NN cord-320015-lbr2q4qh 9 20 of of IN cord-320015-lbr2q4qh 9 21 viral viral JJ cord-320015-lbr2q4qh 9 22 transcription transcription NN cord-320015-lbr2q4qh 9 23 , , , cord-320015-lbr2q4qh 9 24 genome genome NN cord-320015-lbr2q4qh 9 25 replication replication NN cord-320015-lbr2q4qh 9 26 , , , cord-320015-lbr2q4qh 9 27 and and CC cord-320015-lbr2q4qh 9 28 virus virus NN cord-320015-lbr2q4qh 9 29 - - HYPH cord-320015-lbr2q4qh 9 30 mediated mediate VBN cord-320015-lbr2q4qh 9 31 host host NN cord-320015-lbr2q4qh 9 32 - - HYPH cord-320015-lbr2q4qh 9 33 shutoff shutoff NN cord-320015-lbr2q4qh 9 34 . . . cord-320015-lbr2q4qh 10 1 Although although IN cord-320015-lbr2q4qh 10 2 the the DT cord-320015-lbr2q4qh 10 3 broad broad JJ cord-320015-lbr2q4qh 10 4 outlines outline NNS cord-320015-lbr2q4qh 10 5 of of IN cord-320015-lbr2q4qh 10 6 FV3 FV3 NNP cord-320015-lbr2q4qh 10 7 replication replication NN cord-320015-lbr2q4qh 10 8 have have VBP cord-320015-lbr2q4qh 10 9 been be VBN cord-320015-lbr2q4qh 10 10 elucidated elucidate VBN cord-320015-lbr2q4qh 10 11 , , , cord-320015-lbr2q4qh 10 12 the the DT cord-320015-lbr2q4qh 10 13 precise precise JJ cord-320015-lbr2q4qh 10 14 roles role NNS cord-320015-lbr2q4qh 10 15 of of IN cord-320015-lbr2q4qh 10 16 most most JJS cord-320015-lbr2q4qh 10 17 viral viral JJ cord-320015-lbr2q4qh 10 18 proteins protein NNS cord-320015-lbr2q4qh 10 19 remain remain VBP cord-320015-lbr2q4qh 10 20 unknown unknown JJ cord-320015-lbr2q4qh 10 21 . . . cord-320015-lbr2q4qh 11 1 Current current JJ cord-320015-lbr2q4qh 11 2 studies study NNS cord-320015-lbr2q4qh 11 3 using use VBG cord-320015-lbr2q4qh 11 4 knock knock VB cord-320015-lbr2q4qh 11 5 down down RP cord-320015-lbr2q4qh 11 6 ( ( -LRB- cord-320015-lbr2q4qh 11 7 KD KD NNP cord-320015-lbr2q4qh 11 8 ) ) -RRB- cord-320015-lbr2q4qh 11 9 mediated mediate VBN cord-320015-lbr2q4qh 11 10 by by IN cord-320015-lbr2q4qh 11 11 antisense antisense NN cord-320015-lbr2q4qh 11 12 morpholino morpholino NNP cord-320015-lbr2q4qh 11 13 oligonucleotides oligonucleotide NNS cord-320015-lbr2q4qh 11 14 ( ( -LRB- cord-320015-lbr2q4qh 11 15 asMO asmo NN cord-320015-lbr2q4qh 11 16 ) ) -RRB- cord-320015-lbr2q4qh 11 17 and and CC cord-320015-lbr2q4qh 11 18 small small JJ cord-320015-lbr2q4qh 11 19 , , , cord-320015-lbr2q4qh 11 20 interfering interfere VBG cord-320015-lbr2q4qh 11 21 RNAs rna NNS cord-320015-lbr2q4qh 11 22 ( ( -LRB- cord-320015-lbr2q4qh 11 23 siRNA siRNA NNP cord-320015-lbr2q4qh 11 24 ) ) -RRB- cord-320015-lbr2q4qh 11 25 , , , cord-320015-lbr2q4qh 11 26 knock knock VB cord-320015-lbr2q4qh 11 27 out out RP cord-320015-lbr2q4qh 11 28 ( ( -LRB- cord-320015-lbr2q4qh 11 29 KO KO NNP cord-320015-lbr2q4qh 11 30 ) ) -RRB- cord-320015-lbr2q4qh 11 31 following follow VBG cord-320015-lbr2q4qh 11 32 replacement replacement NN cord-320015-lbr2q4qh 11 33 of of IN cord-320015-lbr2q4qh 11 34 the the DT cord-320015-lbr2q4qh 11 35 targeted target VBN cord-320015-lbr2q4qh 11 36 gene gene NN cord-320015-lbr2q4qh 11 37 with with IN cord-320015-lbr2q4qh 11 38 a a DT cord-320015-lbr2q4qh 11 39 selectable selectable JJ cord-320015-lbr2q4qh 11 40 marker marker NN cord-320015-lbr2q4qh 11 41 by by IN cord-320015-lbr2q4qh 11 42 homologous homologous JJ cord-320015-lbr2q4qh 11 43 recombination recombination NN cord-320015-lbr2q4qh 11 44 , , , cord-320015-lbr2q4qh 11 45 ectopic ectopic JJ cord-320015-lbr2q4qh 11 46 viral viral JJ cord-320015-lbr2q4qh 11 47 gene gene NN cord-320015-lbr2q4qh 11 48 expression expression NN cord-320015-lbr2q4qh 11 49 , , , cord-320015-lbr2q4qh 11 50 and and CC cord-320015-lbr2q4qh 11 51 recombinant recombinant JJ cord-320015-lbr2q4qh 11 52 viral viral JJ cord-320015-lbr2q4qh 11 53 proteins protein NNS cord-320015-lbr2q4qh 11 54 have have VBP cord-320015-lbr2q4qh 11 55 enabled enable VBN cord-320015-lbr2q4qh 11 56 researchers researcher NNS cord-320015-lbr2q4qh 11 57 to to TO cord-320015-lbr2q4qh 11 58 systematically systematically RB cord-320015-lbr2q4qh 11 59 ascertain ascertain VB cord-320015-lbr2q4qh 11 60 replicative- replicative- NN cord-320015-lbr2q4qh 11 61 and and CC cord-320015-lbr2q4qh 11 62 virulence virulence NN cord-320015-lbr2q4qh 11 63 - - HYPH cord-320015-lbr2q4qh 11 64 related relate VBN cord-320015-lbr2q4qh 11 65 gene gene NN cord-320015-lbr2q4qh 11 66 functions function NNS cord-320015-lbr2q4qh 11 67 . . . cord-320015-lbr2q4qh 12 1 In in IN cord-320015-lbr2q4qh 12 2 addition addition NN cord-320015-lbr2q4qh 12 3 , , , cord-320015-lbr2q4qh 12 4 the the DT cord-320015-lbr2q4qh 12 5 application application NN cord-320015-lbr2q4qh 12 6 of of IN cord-320015-lbr2q4qh 12 7 molecular molecular JJ cord-320015-lbr2q4qh 12 8 tools tool NNS cord-320015-lbr2q4qh 12 9 to to IN cord-320015-lbr2q4qh 12 10 ecological ecological JJ cord-320015-lbr2q4qh 12 11 studies study NNS cord-320015-lbr2q4qh 12 12 is be VBZ cord-320015-lbr2q4qh 12 13 providing provide VBG cord-320015-lbr2q4qh 12 14 novel novel JJ cord-320015-lbr2q4qh 12 15 ways way NNS cord-320015-lbr2q4qh 12 16 for for IN cord-320015-lbr2q4qh 12 17 field field NN cord-320015-lbr2q4qh 12 18 biologists biologist NNS cord-320015-lbr2q4qh 12 19 to to TO cord-320015-lbr2q4qh 12 20 identify identify VB cord-320015-lbr2q4qh 12 21 potential potential JJ cord-320015-lbr2q4qh 12 22 pathogens pathogen NNS cord-320015-lbr2q4qh 12 23 , , , cord-320015-lbr2q4qh 12 24 quantify quantify VB cord-320015-lbr2q4qh 12 25 infections infection NNS cord-320015-lbr2q4qh 12 26 , , , cord-320015-lbr2q4qh 12 27 and and CC cord-320015-lbr2q4qh 12 28 trace trace VB cord-320015-lbr2q4qh 12 29 the the DT cord-320015-lbr2q4qh 12 30 evolution evolution NN cord-320015-lbr2q4qh 12 31 of of IN cord-320015-lbr2q4qh 12 32 ecologically ecologically RB cord-320015-lbr2q4qh 12 33 important important JJ cord-320015-lbr2q4qh 12 34 viral viral JJ cord-320015-lbr2q4qh 12 35 species specie NNS cord-320015-lbr2q4qh 12 36 . . . cord-320015-lbr2q4qh 13 1 In in IN cord-320015-lbr2q4qh 13 2 this this DT cord-320015-lbr2q4qh 13 3 review review NN cord-320015-lbr2q4qh 13 4 , , , cord-320015-lbr2q4qh 13 5 we -PRON- PRP cord-320015-lbr2q4qh 13 6 summarize summarize VBP cord-320015-lbr2q4qh 13 7 current current JJ cord-320015-lbr2q4qh 13 8 studies study NNS cord-320015-lbr2q4qh 13 9 using use VBG cord-320015-lbr2q4qh 13 10 not not RB cord-320015-lbr2q4qh 13 11 only only RB cord-320015-lbr2q4qh 13 12 FV3 FV3 NNP cord-320015-lbr2q4qh 13 13 , , , cord-320015-lbr2q4qh 13 14 but but CC cord-320015-lbr2q4qh 13 15 also also RB cord-320015-lbr2q4qh 13 16 other other JJ cord-320015-lbr2q4qh 13 17 iridoviruses iridoviruse NNS cord-320015-lbr2q4qh 13 18 infecting infect VBG cord-320015-lbr2q4qh 13 19 ectotherms ectotherm NNS cord-320015-lbr2q4qh 13 20 . . . cord-320015-lbr2q4qh 14 1 As as IN cord-320015-lbr2q4qh 14 2 described describe VBN cord-320015-lbr2q4qh 14 3 below below RB cord-320015-lbr2q4qh 14 4 , , , cord-320015-lbr2q4qh 14 5 general general JJ cord-320015-lbr2q4qh 14 6 principles principle NNS cord-320015-lbr2q4qh 14 7 ascertained ascertain VBD cord-320015-lbr2q4qh 14 8 using use VBG cord-320015-lbr2q4qh 14 9 FV3 FV3 NNP cord-320015-lbr2q4qh 14 10 served serve VBD cord-320015-lbr2q4qh 14 11 as as IN cord-320015-lbr2q4qh 14 12 a a DT cord-320015-lbr2q4qh 14 13 model model NN cord-320015-lbr2q4qh 14 14 for for IN cord-320015-lbr2q4qh 14 15 the the DT cord-320015-lbr2q4qh 14 16 family family NN cord-320015-lbr2q4qh 14 17 , , , cord-320015-lbr2q4qh 14 18 and and CC cord-320015-lbr2q4qh 14 19 studies study NNS cord-320015-lbr2q4qh 14 20 utilizing utilize VBG cord-320015-lbr2q4qh 14 21 other other JJ cord-320015-lbr2q4qh 14 22 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 14 23 and and CC cord-320015-lbr2q4qh 14 24 megalocytiviruses megalocytiviruse NNS cord-320015-lbr2q4qh 14 25 have have VBP cord-320015-lbr2q4qh 14 26 confirmed confirm VBN cord-320015-lbr2q4qh 14 27 and and CC cord-320015-lbr2q4qh 14 28 extended extend VBN cord-320015-lbr2q4qh 14 29 our -PRON- PRP$ cord-320015-lbr2q4qh 14 30 understanding understanding NN cord-320015-lbr2q4qh 14 31 of of IN cord-320015-lbr2q4qh 14 32 iridovirus iridovirus JJ cord-320015-lbr2q4qh 14 33 replication replication NN cord-320015-lbr2q4qh 14 34 . . . cord-320015-lbr2q4qh 15 1 Collectively collectively RB cord-320015-lbr2q4qh 15 2 , , , cord-320015-lbr2q4qh 15 3 these these DT cord-320015-lbr2q4qh 15 4 and and CC cord-320015-lbr2q4qh 15 5 future future JJ cord-320015-lbr2q4qh 15 6 efforts effort NNS cord-320015-lbr2q4qh 15 7 will will MD cord-320015-lbr2q4qh 15 8 elucidate elucidate VB cord-320015-lbr2q4qh 15 9 molecular molecular JJ cord-320015-lbr2q4qh 15 10 events event NNS cord-320015-lbr2q4qh 15 11 in in IN cord-320015-lbr2q4qh 15 12 viral viral JJ cord-320015-lbr2q4qh 15 13 replication replication NN cord-320015-lbr2q4qh 15 14 , , , cord-320015-lbr2q4qh 15 15 intrinsic intrinsic JJ cord-320015-lbr2q4qh 15 16 and and CC cord-320015-lbr2q4qh 15 17 extrinsic extrinsic JJ cord-320015-lbr2q4qh 15 18 factors factor NNS cord-320015-lbr2q4qh 15 19 that that WDT cord-320015-lbr2q4qh 15 20 contribute contribute VBP cord-320015-lbr2q4qh 15 21 to to IN cord-320015-lbr2q4qh 15 22 disease disease NN cord-320015-lbr2q4qh 15 23 outbreaks outbreak NNS cord-320015-lbr2q4qh 15 24 , , , cord-320015-lbr2q4qh 15 25 and and CC cord-320015-lbr2q4qh 15 26 the the DT cord-320015-lbr2q4qh 15 27 role role NN cord-320015-lbr2q4qh 15 28 of of IN cord-320015-lbr2q4qh 15 29 the the DT cord-320015-lbr2q4qh 15 30 host host NN cord-320015-lbr2q4qh 15 31 immune immune JJ cord-320015-lbr2q4qh 15 32 system system NN cord-320015-lbr2q4qh 15 33 in in IN cord-320015-lbr2q4qh 15 34 protection protection NN cord-320015-lbr2q4qh 15 35 from from IN cord-320015-lbr2q4qh 15 36 disease disease NN cord-320015-lbr2q4qh 15 37 . . . cord-320015-lbr2q4qh 16 1 : : : cord-320015-lbr2q4qh 17 1 Sizes size NNS cord-320015-lbr2q4qh 17 2 and and CC cord-320015-lbr2q4qh 17 3 coding code VBG cord-320015-lbr2q4qh 17 4 potentials potential NNS cord-320015-lbr2q4qh 17 5 of of IN cord-320015-lbr2q4qh 17 6 iridovirid iridovirid NN cord-320015-lbr2q4qh 17 7 genomes genome NNS cord-320015-lbr2q4qh 17 8 . . . cord-320015-lbr2q4qh 18 1 Iridovirus Iridovirus NNP cord-320015-lbr2q4qh 18 2 _SP cord-320015-lbr2q4qh 19 1 IIV-9 IIV-9 NNP cord-320015-lbr2q4qh 19 2 _SP cord-320015-lbr2q4qh 20 1 206,791 206,791 CD cord-320015-lbr2q4qh 20 2 _SP cord-320015-lbr2q4qh 21 1 191 191 CD cord-320015-lbr2q4qh 21 2 _SP cord-320015-lbr2q4qh 22 1 31 31 CD cord-320015-lbr2q4qh 22 2 _SP cord-320015-lbr2q4qh 23 1 GQ918152 GQ918152 NNP cord-320015-lbr2q4qh 23 2 _SP cord-320015-lbr2q4qh 24 1 IIV-6 IIV-6 NNP cord-320015-lbr2q4qh 24 2 _SP cord-320015-lbr2q4qh 25 1 Aside aside RB cord-320015-lbr2q4qh 25 2 from from IN cord-320015-lbr2q4qh 25 3 differences difference NNS cord-320015-lbr2q4qh 25 4 in in IN cord-320015-lbr2q4qh 25 5 virion virion NN cord-320015-lbr2q4qh 25 6 and and CC cord-320015-lbr2q4qh 25 7 genome genome NN cord-320015-lbr2q4qh 25 8 sizes size NNS cord-320015-lbr2q4qh 25 9 , , , cord-320015-lbr2q4qh 25 10 the the DT cord-320015-lbr2q4qh 25 11 organization organization NN cord-320015-lbr2q4qh 25 12 of of IN cord-320015-lbr2q4qh 25 13 virus virus NN cord-320015-lbr2q4qh 25 14 particles particle NNS cord-320015-lbr2q4qh 25 15 within within IN cord-320015-lbr2q4qh 25 16 the the DT cord-320015-lbr2q4qh 25 17 family family NN cord-320015-lbr2q4qh 25 18 is be VBZ cord-320015-lbr2q4qh 25 19 generally generally RB cord-320015-lbr2q4qh 25 20 similar similar JJ cord-320015-lbr2q4qh 25 21 . . . cord-320015-lbr2q4qh 26 1 While while IN cord-320015-lbr2q4qh 26 2 transmission transmission NN cord-320015-lbr2q4qh 26 3 electron electron NN cord-320015-lbr2q4qh 26 4 microscopy microscopy NN cord-320015-lbr2q4qh 26 5 ( ( -LRB- cord-320015-lbr2q4qh 26 6 TEM TEM NNP cord-320015-lbr2q4qh 26 7 ) ) -RRB- cord-320015-lbr2q4qh 26 8 has have VBZ cord-320015-lbr2q4qh 26 9 been be VBN cord-320015-lbr2q4qh 26 10 conducted conduct VBN cord-320015-lbr2q4qh 26 11 using use VBG cord-320015-lbr2q4qh 26 12 FV3 FV3 NNP cord-320015-lbr2q4qh 26 13 and and CC cord-320015-lbr2q4qh 26 14 other other JJ cord-320015-lbr2q4qh 26 15 iridovirids iridovirid NNS cord-320015-lbr2q4qh 26 16 , , , cord-320015-lbr2q4qh 26 17 detailed detailed JJ cord-320015-lbr2q4qh 26 18 cryo cryo NN cord-320015-lbr2q4qh 26 19 - - HYPH cord-320015-lbr2q4qh 26 20 electron electron NN cord-320015-lbr2q4qh 26 21 microscopic microscopic NN cord-320015-lbr2q4qh 26 22 ( ( -LRB- cord-320015-lbr2q4qh 26 23 CryoEM CryoEM NNP cord-320015-lbr2q4qh 26 24 ) ) -RRB- cord-320015-lbr2q4qh 26 25 analyses analysis NNS cord-320015-lbr2q4qh 26 26 ( ( -LRB- cord-320015-lbr2q4qh 26 27 discussed discuss VBN cord-320015-lbr2q4qh 26 28 below below RB cord-320015-lbr2q4qh 26 29 ) ) -RRB- cord-320015-lbr2q4qh 26 30 were be VBD cord-320015-lbr2q4qh 26 31 performed perform VBN cord-320015-lbr2q4qh 26 32 with with IN cord-320015-lbr2q4qh 26 33 IIV-6 IIV-6 NNP cord-320015-lbr2q4qh 26 34 and and CC cord-320015-lbr2q4qh 26 35 provide provide VB cord-320015-lbr2q4qh 26 36 the the DT cord-320015-lbr2q4qh 26 37 most most RBS cord-320015-lbr2q4qh 26 38 detailed detailed JJ cord-320015-lbr2q4qh 26 39 view view NN cord-320015-lbr2q4qh 26 40 of of IN cord-320015-lbr2q4qh 26 41 virion virion NN cord-320015-lbr2q4qh 26 42 structure structure NN cord-320015-lbr2q4qh 26 43 . . . cord-320015-lbr2q4qh 27 1 Virions virion NNS cord-320015-lbr2q4qh 27 2 exist exist VBP cord-320015-lbr2q4qh 27 3 in in IN cord-320015-lbr2q4qh 27 4 two two CD cord-320015-lbr2q4qh 27 5 forms form NNS cord-320015-lbr2q4qh 27 6 : : : cord-320015-lbr2q4qh 27 7 non non JJ cord-320015-lbr2q4qh 27 8 - - JJ cord-320015-lbr2q4qh 27 9 enveloped enveloped JJ cord-320015-lbr2q4qh 27 10 particles particle NNS cord-320015-lbr2q4qh 27 11 ~120 ~120 XX cord-320015-lbr2q4qh 27 12 - - HYPH cord-320015-lbr2q4qh 27 13 180 180 CD cord-320015-lbr2q4qh 27 14 nm nm NN cord-320015-lbr2q4qh 27 15 in in IN cord-320015-lbr2q4qh 27 16 diameter diameter NN cord-320015-lbr2q4qh 27 17 , , , cord-320015-lbr2q4qh 27 18 and and CC cord-320015-lbr2q4qh 27 19 enveloped envelop VBD cord-320015-lbr2q4qh 27 20 particles particle NNS cord-320015-lbr2q4qh 27 21 that that WDT cord-320015-lbr2q4qh 27 22 acquire acquire VBP cord-320015-lbr2q4qh 27 23 an an DT cord-320015-lbr2q4qh 27 24 envelope envelope NN cord-320015-lbr2q4qh 27 25 by by IN cord-320015-lbr2q4qh 27 26 budding bud VBG cord-320015-lbr2q4qh 27 27 from from IN cord-320015-lbr2q4qh 27 28 the the DT cord-320015-lbr2q4qh 27 29 plasma plasma NN cord-320015-lbr2q4qh 27 30 membrane membrane NN cord-320015-lbr2q4qh 27 31 . . . cord-320015-lbr2q4qh 28 1 In in IN cord-320015-lbr2q4qh 28 2 addition addition NN cord-320015-lbr2q4qh 28 3 to to IN cord-320015-lbr2q4qh 28 4 the the DT cord-320015-lbr2q4qh 28 5 external external JJ cord-320015-lbr2q4qh 28 6 envelope envelope NN cord-320015-lbr2q4qh 28 7 , , , cord-320015-lbr2q4qh 28 8 some some DT cord-320015-lbr2q4qh 28 9 iridovirids iridovirid NNS cord-320015-lbr2q4qh 28 10 contain contain VBP cord-320015-lbr2q4qh 28 11 a a DT cord-320015-lbr2q4qh 28 12 fringe fringe NN cord-320015-lbr2q4qh 28 13 of of IN cord-320015-lbr2q4qh 28 14 fine fine JJ cord-320015-lbr2q4qh 28 15 fibers fiber NNS cord-320015-lbr2q4qh 28 16 ( ( -LRB- cord-320015-lbr2q4qh 28 17 fibrils fibril NNS cord-320015-lbr2q4qh 28 18 ) ) -RRB- cord-320015-lbr2q4qh 28 19 that that WDT cord-320015-lbr2q4qh 28 20 extend extend VBP cord-320015-lbr2q4qh 28 21 outward outward RB cord-320015-lbr2q4qh 28 22 from from IN cord-320015-lbr2q4qh 28 23 the the DT cord-320015-lbr2q4qh 28 24 capsid capsid NN cord-320015-lbr2q4qh 28 25 subunits subunit NNS cord-320015-lbr2q4qh 28 26 [ [ -LRB- cord-320015-lbr2q4qh 28 27 15 15 CD cord-320015-lbr2q4qh 28 28 , , , cord-320015-lbr2q4qh 28 29 16 16 CD cord-320015-lbr2q4qh 28 30 ] ] -RRB- cord-320015-lbr2q4qh 28 31 . . . cord-320015-lbr2q4qh 29 1 Non Non NNS cord-320015-lbr2q4qh 29 2 - - JJ cord-320015-lbr2q4qh 29 3 enveloped enveloped JJ cord-320015-lbr2q4qh 29 4 particles particle NNS cord-320015-lbr2q4qh 29 5 are be VBP cord-320015-lbr2q4qh 29 6 composed compose VBN cord-320015-lbr2q4qh 29 7 of of IN cord-320015-lbr2q4qh 29 8 three three CD cord-320015-lbr2q4qh 29 9 distinct distinct JJ cord-320015-lbr2q4qh 29 10 layers layer NNS cord-320015-lbr2q4qh 29 11 : : : cord-320015-lbr2q4qh 29 12 an an DT cord-320015-lbr2q4qh 29 13 outer outer JJ cord-320015-lbr2q4qh 29 14 capsid capsid NN cord-320015-lbr2q4qh 29 15 composed compose VBN cord-320015-lbr2q4qh 29 16 of of IN cord-320015-lbr2q4qh 29 17 multiple multiple JJ cord-320015-lbr2q4qh 29 18 copies copy NNS cord-320015-lbr2q4qh 29 19 of of IN cord-320015-lbr2q4qh 29 20 an an DT cord-320015-lbr2q4qh 29 21 ~50 ~50 XX cord-320015-lbr2q4qh 29 22 kDa kDa NNP cord-320015-lbr2q4qh 29 23 major major JJ cord-320015-lbr2q4qh 29 24 capsid capsid NN cord-320015-lbr2q4qh 29 25 protein protein NN cord-320015-lbr2q4qh 29 26 ( ( -LRB- cord-320015-lbr2q4qh 29 27 MCP MCP NNP cord-320015-lbr2q4qh 29 28 ) ) -RRB- cord-320015-lbr2q4qh 29 29 , , , cord-320015-lbr2q4qh 29 30 an an DT cord-320015-lbr2q4qh 29 31 internal internal JJ cord-320015-lbr2q4qh 29 32 lipid lipid NN cord-320015-lbr2q4qh 29 33 membrane membrane NN cord-320015-lbr2q4qh 29 34 possibly possibly RB cord-320015-lbr2q4qh 29 35 derived derive VBN cord-320015-lbr2q4qh 29 36 from from IN cord-320015-lbr2q4qh 29 37 the the DT cord-320015-lbr2q4qh 29 38 endoplasmic endoplasmic NN cord-320015-lbr2q4qh 29 39 reticulum reticulum NN cord-320015-lbr2q4qh 29 40 ( ( -LRB- cord-320015-lbr2q4qh 29 41 ER ER NNP cord-320015-lbr2q4qh 29 42 ) ) -RRB- cord-320015-lbr2q4qh 29 43 or or CC cord-320015-lbr2q4qh 29 44 other other JJ cord-320015-lbr2q4qh 29 45 cellular cellular JJ cord-320015-lbr2q4qh 29 46 membranes membrane NNS cord-320015-lbr2q4qh 29 47 , , , cord-320015-lbr2q4qh 29 48 and and CC cord-320015-lbr2q4qh 29 49 an an DT cord-320015-lbr2q4qh 29 50 inner inner JJ cord-320015-lbr2q4qh 29 51 core core NN cord-320015-lbr2q4qh 29 52 containing contain VBG cord-320015-lbr2q4qh 29 53 the the DT cord-320015-lbr2q4qh 29 54 viral viral JJ cord-320015-lbr2q4qh 29 55 genome genome NN cord-320015-lbr2q4qh 29 56 and and CC cord-320015-lbr2q4qh 29 57 associated associated JJ cord-320015-lbr2q4qh 29 58 virus virus NN cord-320015-lbr2q4qh 29 59 - - HYPH cord-320015-lbr2q4qh 29 60 encoded encode VBN cord-320015-lbr2q4qh 29 61 proteins protein NNS cord-320015-lbr2q4qh 29 62 [ [ -LRB- cord-320015-lbr2q4qh 29 63 16 16 CD cord-320015-lbr2q4qh 29 64 , , , cord-320015-lbr2q4qh 29 65 17 17 CD cord-320015-lbr2q4qh 29 66 ] ] -RRB- cord-320015-lbr2q4qh 29 67 . . . cord-320015-lbr2q4qh 30 1 The the DT cord-320015-lbr2q4qh 30 2 MCP MCP NNP cord-320015-lbr2q4qh 30 3 shows show VBZ cord-320015-lbr2q4qh 30 4 marked marked JJ cord-320015-lbr2q4qh 30 5 sequence sequence NN cord-320015-lbr2q4qh 30 6 conservation conservation NN cord-320015-lbr2q4qh 30 7 within within IN cord-320015-lbr2q4qh 30 8 all all DT cord-320015-lbr2q4qh 30 9 members member NNS cord-320015-lbr2q4qh 30 10 of of IN cord-320015-lbr2q4qh 30 11 the the DT cord-320015-lbr2q4qh 30 12 family family NN cord-320015-lbr2q4qh 30 13 [ [ -LRB- cord-320015-lbr2q4qh 30 14 18 18 CD cord-320015-lbr2q4qh 30 15 ] ] -RRB- cord-320015-lbr2q4qh 30 16 . . . cord-320015-lbr2q4qh 31 1 Sequence sequence NN cord-320015-lbr2q4qh 31 2 identity identity NN cord-320015-lbr2q4qh 31 3 within within IN cord-320015-lbr2q4qh 31 4 the the DT cord-320015-lbr2q4qh 31 5 MCP MCP NNP cord-320015-lbr2q4qh 31 6 gene gene NN cord-320015-lbr2q4qh 31 7 allows allow VBZ cord-320015-lbr2q4qh 31 8 primer primer NN cord-320015-lbr2q4qh 31 9 - - HYPH cord-320015-lbr2q4qh 31 10 based base VBN cord-320015-lbr2q4qh 31 11 PCR PCR NNP cord-320015-lbr2q4qh 31 12 amplification amplification NN cord-320015-lbr2q4qh 31 13 of of IN cord-320015-lbr2q4qh 31 14 viral viral JJ cord-320015-lbr2q4qh 31 15 DNA dna NN cord-320015-lbr2q4qh 31 16 and and CC cord-320015-lbr2q4qh 31 17 provides provide VBZ cord-320015-lbr2q4qh 31 18 an an DT cord-320015-lbr2q4qh 31 19 easy easy JJ cord-320015-lbr2q4qh 31 20 way way NN cord-320015-lbr2q4qh 31 21 to to TO cord-320015-lbr2q4qh 31 22 determine determine VB cord-320015-lbr2q4qh 31 23 whether whether IN cord-320015-lbr2q4qh 31 24 a a DT cord-320015-lbr2q4qh 31 25 given give VBN cord-320015-lbr2q4qh 31 26 virus virus NN cord-320015-lbr2q4qh 31 27 is be VBZ cord-320015-lbr2q4qh 31 28 a a DT cord-320015-lbr2q4qh 31 29 member member NN cord-320015-lbr2q4qh 31 30 of of IN cord-320015-lbr2q4qh 31 31 the the DT cord-320015-lbr2q4qh 31 32 family family NN cord-320015-lbr2q4qh 31 33 and and CC cord-320015-lbr2q4qh 31 34 to to TO cord-320015-lbr2q4qh 31 35 which which WDT cord-320015-lbr2q4qh 31 36 genera genera VBP cord-320015-lbr2q4qh 31 37 it -PRON- PRP cord-320015-lbr2q4qh 31 38 belongs belong VBZ cord-320015-lbr2q4qh 31 39 [ [ -LRB- cord-320015-lbr2q4qh 31 40 19 19 CD cord-320015-lbr2q4qh 31 41 ] ] -RRB- cord-320015-lbr2q4qh 31 42 . . . cord-320015-lbr2q4qh 32 1 Although although IN cord-320015-lbr2q4qh 32 2 the the DT cord-320015-lbr2q4qh 32 3 MCP MCP NNP cord-320015-lbr2q4qh 32 4 is be VBZ cord-320015-lbr2q4qh 32 5 the the DT cord-320015-lbr2q4qh 32 6 primary primary JJ cord-320015-lbr2q4qh 32 7 structural structural JJ cord-320015-lbr2q4qh 32 8 protein protein NN cord-320015-lbr2q4qh 32 9 and and CC cord-320015-lbr2q4qh 32 10 comprises comprise VBZ cord-320015-lbr2q4qh 32 11 40 40 CD cord-320015-lbr2q4qh 32 12 % % NN cord-320015-lbr2q4qh 32 13 of of IN cord-320015-lbr2q4qh 32 14 the the DT cord-320015-lbr2q4qh 32 15 total total JJ cord-320015-lbr2q4qh 32 16 virion virion NN cord-320015-lbr2q4qh 32 17 protein protein NN cord-320015-lbr2q4qh 32 18 content content NN cord-320015-lbr2q4qh 32 19 , , , cord-320015-lbr2q4qh 32 20 an an DT cord-320015-lbr2q4qh 32 21 additional additional JJ cord-320015-lbr2q4qh 32 22 36 36 CD cord-320015-lbr2q4qh 32 23 proteins protein NNS cord-320015-lbr2q4qh 32 24 have have VBP cord-320015-lbr2q4qh 32 25 been be VBN cord-320015-lbr2q4qh 32 26 identified identify VBN cord-320015-lbr2q4qh 32 27 following follow VBG cord-320015-lbr2q4qh 32 28 gel gel NN cord-320015-lbr2q4qh 32 29 analysis analysis NN cord-320015-lbr2q4qh 32 30 of of IN cord-320015-lbr2q4qh 32 31 purified purify VBN cord-320015-lbr2q4qh 32 32 virions virion NNS cord-320015-lbr2q4qh 32 33 [ [ -LRB- cord-320015-lbr2q4qh 32 34 3 3 CD cord-320015-lbr2q4qh 32 35 ] ] -RRB- cord-320015-lbr2q4qh 32 36 . . . cord-320015-lbr2q4qh 33 1 While while IN cord-320015-lbr2q4qh 33 2 many many JJ cord-320015-lbr2q4qh 33 3 of of IN cord-320015-lbr2q4qh 33 4 these these DT cord-320015-lbr2q4qh 33 5 proteins protein NNS cord-320015-lbr2q4qh 33 6 likely likely RB cord-320015-lbr2q4qh 33 7 represent represent VBP cord-320015-lbr2q4qh 33 8 virus virus NN cord-320015-lbr2q4qh 33 9 - - HYPH cord-320015-lbr2q4qh 33 10 encoded encode VBN cord-320015-lbr2q4qh 33 11 catalytic catalytic JJ cord-320015-lbr2q4qh 33 12 proteins protein NNS cord-320015-lbr2q4qh 33 13 that that WDT cord-320015-lbr2q4qh 33 14 play play VBP cord-320015-lbr2q4qh 33 15 various various JJ cord-320015-lbr2q4qh 33 16 roles role NNS cord-320015-lbr2q4qh 33 17 in in IN cord-320015-lbr2q4qh 33 18 replication replication NN cord-320015-lbr2q4qh 33 19 , , , cord-320015-lbr2q4qh 33 20 three three CD cord-320015-lbr2q4qh 33 21 minor minor JJ cord-320015-lbr2q4qh 33 22 capsid capsid NN cord-320015-lbr2q4qh 33 23 proteins protein NNS cord-320015-lbr2q4qh 33 24 have have VBP cord-320015-lbr2q4qh 33 25 been be VBN cord-320015-lbr2q4qh 33 26 identified identify VBN cord-320015-lbr2q4qh 33 27 by by IN cord-320015-lbr2q4qh 33 28 CryoEM CryoEM NNP cord-320015-lbr2q4qh 33 29 analysis analysis NN cord-320015-lbr2q4qh 33 30 of of IN cord-320015-lbr2q4qh 33 31 IIV-6 IIV-6 NNP cord-320015-lbr2q4qh 33 32 and and CC cord-320015-lbr2q4qh 33 33 designated designate VBN cord-320015-lbr2q4qh 33 34 as as IN cord-320015-lbr2q4qh 33 35 finger finger NN cord-320015-lbr2q4qh 33 36 , , , cord-320015-lbr2q4qh 33 37 zip zip NN cord-320015-lbr2q4qh 33 38 , , , cord-320015-lbr2q4qh 33 39 and and CC cord-320015-lbr2q4qh 33 40 anchor anchor NN cord-320015-lbr2q4qh 33 41 proteins protein NNS cord-320015-lbr2q4qh 33 42 [ [ -LRB- cord-320015-lbr2q4qh 33 43 17 17 CD cord-320015-lbr2q4qh 33 44 ] ] -RRB- cord-320015-lbr2q4qh 33 45 . . . cord-320015-lbr2q4qh 34 1 Finger finger NN cord-320015-lbr2q4qh 34 2 and and CC cord-320015-lbr2q4qh 34 3 zip zip NN cord-320015-lbr2q4qh 34 4 proteins protein NNS cord-320015-lbr2q4qh 34 5 were be VBD cord-320015-lbr2q4qh 34 6 suggested suggest VBN cord-320015-lbr2q4qh 34 7 to to TO cord-320015-lbr2q4qh 34 8 stabilize stabilize VB cord-320015-lbr2q4qh 34 9 the the DT cord-320015-lbr2q4qh 34 10 virus virus NN cord-320015-lbr2q4qh 34 11 by by IN cord-320015-lbr2q4qh 34 12 acting act VBG cord-320015-lbr2q4qh 34 13 as as IN cord-320015-lbr2q4qh 34 14 intercapsomer intercapsomer NNP cord-320015-lbr2q4qh 34 15 cross cross NN cord-320015-lbr2q4qh 34 16 - - NNS cord-320015-lbr2q4qh 34 17 links link NNS cord-320015-lbr2q4qh 34 18 , , , cord-320015-lbr2q4qh 34 19 whereas whereas IN cord-320015-lbr2q4qh 34 20 the the DT cord-320015-lbr2q4qh 34 21 anchor anchor NN cord-320015-lbr2q4qh 34 22 proteins protein NNS cord-320015-lbr2q4qh 34 23 appear appear VBP cord-320015-lbr2q4qh 34 24 to to TO cord-320015-lbr2q4qh 34 25 be be VB cord-320015-lbr2q4qh 34 26 transmembrane transmembrane NN cord-320015-lbr2q4qh 34 27 proteins protein NNS cord-320015-lbr2q4qh 34 28 that that WDT cord-320015-lbr2q4qh 34 29 extend extend VBP cord-320015-lbr2q4qh 34 30 into into IN cord-320015-lbr2q4qh 34 31 the the DT cord-320015-lbr2q4qh 34 32 internal internal JJ cord-320015-lbr2q4qh 34 33 lipid lipid NN cord-320015-lbr2q4qh 34 34 membrane membrane NN cord-320015-lbr2q4qh 34 35 and and CC cord-320015-lbr2q4qh 34 36 provide provide VB cord-320015-lbr2q4qh 34 37 further further JJ cord-320015-lbr2q4qh 34 38 stabilization stabilization NN cord-320015-lbr2q4qh 34 39 . . . cord-320015-lbr2q4qh 35 1 In in IN cord-320015-lbr2q4qh 35 2 addition addition NN cord-320015-lbr2q4qh 35 3 to to IN cord-320015-lbr2q4qh 35 4 these these DT cord-320015-lbr2q4qh 35 5 proteins protein NNS cord-320015-lbr2q4qh 35 6 , , , cord-320015-lbr2q4qh 35 7 a a DT cord-320015-lbr2q4qh 35 8 fifth fifth JJ cord-320015-lbr2q4qh 35 9 protein protein NN cord-320015-lbr2q4qh 35 10 , , , cord-320015-lbr2q4qh 35 11 a a DT cord-320015-lbr2q4qh 35 12 putative putative JJ cord-320015-lbr2q4qh 35 13 myristoylated myristoylated JJ cord-320015-lbr2q4qh 35 14 protein protein NN cord-320015-lbr2q4qh 35 15 ( ( -LRB- cord-320015-lbr2q4qh 35 16 FV3 FV3 NNP cord-320015-lbr2q4qh 35 17 ORF ORF NNP cord-320015-lbr2q4qh 35 18 53R 53R NNP cord-320015-lbr2q4qh 35 19 ) ) -RRB- cord-320015-lbr2q4qh 35 20 , , , cord-320015-lbr2q4qh 35 21 has have VBZ cord-320015-lbr2q4qh 35 22 been be VBN cord-320015-lbr2q4qh 35 23 suggested suggest VBN cord-320015-lbr2q4qh 35 24 to to TO cord-320015-lbr2q4qh 35 25 interact interact VB cord-320015-lbr2q4qh 35 26 with with IN cord-320015-lbr2q4qh 35 27 cellular cellular JJ cord-320015-lbr2q4qh 35 28 membrane membrane NN cord-320015-lbr2q4qh 35 29 fragments fragment NNS cord-320015-lbr2q4qh 35 30 and and CC cord-320015-lbr2q4qh 35 31 play play VB cord-320015-lbr2q4qh 35 32 a a DT cord-320015-lbr2q4qh 35 33 role role NN cord-320015-lbr2q4qh 35 34 in in IN cord-320015-lbr2q4qh 35 35 virion virion NN cord-320015-lbr2q4qh 35 36 assembly assembly NN cord-320015-lbr2q4qh 35 37 [ [ -LRB- cord-320015-lbr2q4qh 35 38 20 20 CD cord-320015-lbr2q4qh 35 39 ] ] -RRB- cord-320015-lbr2q4qh 35 40 . . . cord-320015-lbr2q4qh 36 1 As as IN cord-320015-lbr2q4qh 36 2 shown show VBN cord-320015-lbr2q4qh 36 3 in in IN cord-320015-lbr2q4qh 36 4 Table Table NNP cord-320015-lbr2q4qh 36 5 1 1 CD cord-320015-lbr2q4qh 36 6 , , , cord-320015-lbr2q4qh 36 7 iridovirid iridovirid NN cord-320015-lbr2q4qh 36 8 genomes genome NNS cord-320015-lbr2q4qh 36 9 range range VBP cord-320015-lbr2q4qh 36 10 in in IN cord-320015-lbr2q4qh 36 11 size size NN cord-320015-lbr2q4qh 36 12 from from IN cord-320015-lbr2q4qh 36 13 105 105 CD cord-320015-lbr2q4qh 36 14 - - SYM cord-320015-lbr2q4qh 36 15 212 212 CD cord-320015-lbr2q4qh 36 16 kbp kbp NNS cord-320015-lbr2q4qh 36 17 . . . cord-320015-lbr2q4qh 37 1 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 37 2 genomes genome NNS cord-320015-lbr2q4qh 37 3 occupy occupy VBP cord-320015-lbr2q4qh 37 4 the the DT cord-320015-lbr2q4qh 37 5 low low JJ cord-320015-lbr2q4qh 37 6 end end NN cord-320015-lbr2q4qh 37 7 of of IN cord-320015-lbr2q4qh 37 8 this this DT cord-320015-lbr2q4qh 37 9 range range NN cord-320015-lbr2q4qh 37 10 and and CC cord-320015-lbr2q4qh 37 11 fall fall VB cord-320015-lbr2q4qh 37 12 into into IN cord-320015-lbr2q4qh 37 13 three three CD cord-320015-lbr2q4qh 37 14 groups group NNS cord-320015-lbr2q4qh 37 15 : : : cord-320015-lbr2q4qh 38 1 the the DT cord-320015-lbr2q4qh 38 2 genomes genome NNS cord-320015-lbr2q4qh 38 3 of of IN cord-320015-lbr2q4qh 38 4 FV3 FV3 NNP cord-320015-lbr2q4qh 38 5 , , , cord-320015-lbr2q4qh 38 6 ATV ATV NNP cord-320015-lbr2q4qh 38 7 , , , cord-320015-lbr2q4qh 38 8 TFV TFV NNP cord-320015-lbr2q4qh 38 9 , , , cord-320015-lbr2q4qh 38 10 and and CC cord-320015-lbr2q4qh 38 11 STIV STIV NNP cord-320015-lbr2q4qh 38 12 are be VBP cord-320015-lbr2q4qh 38 13 105 105 CD cord-320015-lbr2q4qh 38 14 - - SYM cord-320015-lbr2q4qh 38 15 106 106 CD cord-320015-lbr2q4qh 38 16 kbp kbp NNS cord-320015-lbr2q4qh 38 17 , , , cord-320015-lbr2q4qh 38 18 EHNV EHNV NNP cord-320015-lbr2q4qh 38 19 is be VBZ cord-320015-lbr2q4qh 38 20 127 127 CD cord-320015-lbr2q4qh 38 21 kbp kbp NNS cord-320015-lbr2q4qh 38 22 , , , cord-320015-lbr2q4qh 38 23 whereas whereas IN cord-320015-lbr2q4qh 38 24 SGIV SGIV NNP cord-320015-lbr2q4qh 38 25 and and CC cord-320015-lbr2q4qh 38 26 GIV GIV NNP cord-320015-lbr2q4qh 38 27 , , , cord-320015-lbr2q4qh 38 28 likely likely JJ cord-320015-lbr2q4qh 38 29 isolates isolate NNS cord-320015-lbr2q4qh 38 30 of of IN cord-320015-lbr2q4qh 38 31 the the DT cord-320015-lbr2q4qh 38 32 same same JJ cord-320015-lbr2q4qh 38 33 viral viral JJ cord-320015-lbr2q4qh 38 34 species specie NNS cord-320015-lbr2q4qh 38 35 , , , cord-320015-lbr2q4qh 38 36 are be VBP cord-320015-lbr2q4qh 38 37 140 140 CD cord-320015-lbr2q4qh 38 38 kbp kbp NNS cord-320015-lbr2q4qh 38 39 in in IN cord-320015-lbr2q4qh 38 40 size size NN cord-320015-lbr2q4qh 38 41 . . . cord-320015-lbr2q4qh 39 1 Consistent consistent JJ cord-320015-lbr2q4qh 39 2 with with IN cord-320015-lbr2q4qh 39 3 their -PRON- PRP$ cord-320015-lbr2q4qh 39 4 sizes size NNS cord-320015-lbr2q4qh 39 5 , , , cord-320015-lbr2q4qh 39 6 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 39 7 contain contain VBP cord-320015-lbr2q4qh 39 8 between between IN cord-320015-lbr2q4qh 39 9 92 92 CD cord-320015-lbr2q4qh 39 10 and and CC cord-320015-lbr2q4qh 39 11 139 139 CD cord-320015-lbr2q4qh 39 12 close close RB cord-320015-lbr2q4qh 39 13 - - HYPH cord-320015-lbr2q4qh 39 14 packed packed JJ cord-320015-lbr2q4qh 39 15 , , , cord-320015-lbr2q4qh 39 16 predominantly predominantly RB cord-320015-lbr2q4qh 39 17 non non JJ cord-320015-lbr2q4qh 39 18 - - JJ cord-320015-lbr2q4qh 39 19 overlapping overlapping JJ cord-320015-lbr2q4qh 39 20 ORFs orfs NN cord-320015-lbr2q4qh 39 21 of of IN cord-320015-lbr2q4qh 39 22 50 50 CD cord-320015-lbr2q4qh 39 23 amino amino NN cord-320015-lbr2q4qh 39 24 acids acid NNS cord-320015-lbr2q4qh 39 25 or or CC cord-320015-lbr2q4qh 39 26 greater great JJR cord-320015-lbr2q4qh 39 27 [ [ -LRB- cord-320015-lbr2q4qh 39 28 3 3 CD cord-320015-lbr2q4qh 39 29 , , , cord-320015-lbr2q4qh 39 30 [ [ -LRB- cord-320015-lbr2q4qh 39 31 11 11 CD cord-320015-lbr2q4qh 39 32 ] ] -RRB- cord-320015-lbr2q4qh 39 33 [ [ -LRB- cord-320015-lbr2q4qh 39 34 12 12 CD cord-320015-lbr2q4qh 39 35 ] ] -RRB- cord-320015-lbr2q4qh 39 36 [ [ -LRB- cord-320015-lbr2q4qh 39 37 13 13 CD cord-320015-lbr2q4qh 39 38 ] ] -RRB- cord-320015-lbr2q4qh 39 39 . . . cord-320015-lbr2q4qh 40 1 Repetitive repetitive JJ cord-320015-lbr2q4qh 40 2 regions region NNS cord-320015-lbr2q4qh 40 3 are be VBP cord-320015-lbr2q4qh 40 4 common common JJ cord-320015-lbr2q4qh 40 5 and and CC cord-320015-lbr2q4qh 40 6 may may MD cord-320015-lbr2q4qh 40 7 explain explain VB cord-320015-lbr2q4qh 40 8 the the DT cord-320015-lbr2q4qh 40 9 high high JJ cord-320015-lbr2q4qh 40 10 rate rate NN cord-320015-lbr2q4qh 40 11 of of IN cord-320015-lbr2q4qh 40 12 recombination recombination NN cord-320015-lbr2q4qh 40 13 seen see VBN cord-320015-lbr2q4qh 40 14 within within IN cord-320015-lbr2q4qh 40 15 these these DT cord-320015-lbr2q4qh 40 16 viruses virus NNS cord-320015-lbr2q4qh 40 17 . . . cord-320015-lbr2q4qh 41 1 In in IN cord-320015-lbr2q4qh 41 2 addition addition NN cord-320015-lbr2q4qh 41 3 , , , cord-320015-lbr2q4qh 41 4 a a DT cord-320015-lbr2q4qh 41 5 small small JJ cord-320015-lbr2q4qh 41 6 number number NN cord-320015-lbr2q4qh 41 7 of of IN cord-320015-lbr2q4qh 41 8 putative putative JJ cord-320015-lbr2q4qh 41 9 microRNAs microrna NNS cord-320015-lbr2q4qh 41 10 of of IN cord-320015-lbr2q4qh 41 11 unknown unknown JJ cord-320015-lbr2q4qh 41 12 function function NN cord-320015-lbr2q4qh 41 13 have have VBP cord-320015-lbr2q4qh 41 14 been be VBN cord-320015-lbr2q4qh 41 15 detected detect VBN cord-320015-lbr2q4qh 41 16 [ [ -LRB- cord-320015-lbr2q4qh 41 17 21 21 CD cord-320015-lbr2q4qh 41 18 ] ] -RRB- cord-320015-lbr2q4qh 41 19 . . . cord-320015-lbr2q4qh 42 1 Amino amino NN cord-320015-lbr2q4qh 42 2 acid acid NN cord-320015-lbr2q4qh 42 3 conservation conservation NN cord-320015-lbr2q4qh 42 4 is be VBZ cord-320015-lbr2q4qh 42 5 marked mark VBN cord-320015-lbr2q4qh 42 6 among among IN cord-320015-lbr2q4qh 42 7 iridovirid iridovirid NNP cord-320015-lbr2q4qh 42 8 genes gene NNS cord-320015-lbr2q4qh 42 9 , , , cord-320015-lbr2q4qh 42 10 but but CC cord-320015-lbr2q4qh 42 11 gene gene NN cord-320015-lbr2q4qh 42 12 order order NN cord-320015-lbr2q4qh 42 13 , , , cord-320015-lbr2q4qh 42 14 even even RB cord-320015-lbr2q4qh 42 15 within within IN cord-320015-lbr2q4qh 42 16 members member NNS cord-320015-lbr2q4qh 42 17 of of IN cord-320015-lbr2q4qh 42 18 the the DT cord-320015-lbr2q4qh 42 19 same same JJ cord-320015-lbr2q4qh 42 20 genus genus NN cord-320015-lbr2q4qh 42 21 , , , cord-320015-lbr2q4qh 42 22 is be VBZ cord-320015-lbr2q4qh 42 23 not not RB cord-320015-lbr2q4qh 42 24 conserved conserve VBN cord-320015-lbr2q4qh 42 25 suggesting suggest VBG cord-320015-lbr2q4qh 42 26 that that IN cord-320015-lbr2q4qh 42 27 expression expression NN cord-320015-lbr2q4qh 42 28 is be VBZ cord-320015-lbr2q4qh 42 29 likely likely RB cord-320015-lbr2q4qh 42 30 determined determine VBN cord-320015-lbr2q4qh 42 31 by by IN cord-320015-lbr2q4qh 42 32 individual individual JJ cord-320015-lbr2q4qh 42 33 promoter promoter NN cord-320015-lbr2q4qh 42 34 elements element NNS cord-320015-lbr2q4qh 42 35 closely closely RB cord-320015-lbr2q4qh 42 36 associated associate VBN cord-320015-lbr2q4qh 42 37 with with IN cord-320015-lbr2q4qh 42 38 each each DT cord-320015-lbr2q4qh 42 39 gene gene NN cord-320015-lbr2q4qh 42 40 . . . cord-320015-lbr2q4qh 43 1 Moreover moreover RB cord-320015-lbr2q4qh 43 2 , , , cord-320015-lbr2q4qh 43 3 although although IN cord-320015-lbr2q4qh 43 4 viral viral JJ cord-320015-lbr2q4qh 43 5 genes gene NNS cord-320015-lbr2q4qh 43 6 are be VBP cord-320015-lbr2q4qh 43 7 expressed express VBN cord-320015-lbr2q4qh 43 8 in in IN cord-320015-lbr2q4qh 43 9 an an DT cord-320015-lbr2q4qh 43 10 ordered order VBN cord-320015-lbr2q4qh 43 11 temporal temporal JJ cord-320015-lbr2q4qh 43 12 cascade cascade NN cord-320015-lbr2q4qh 43 13 ( ( -LRB- cord-320015-lbr2q4qh 43 14 consisting consist VBG cord-320015-lbr2q4qh 43 15 of of IN cord-320015-lbr2q4qh 43 16 immediate immediate JJ cord-320015-lbr2q4qh 43 17 early early JJ cord-320015-lbr2q4qh 43 18 ( ( -LRB- cord-320015-lbr2q4qh 43 19 IE IE NNP cord-320015-lbr2q4qh 43 20 ) ) -RRB- cord-320015-lbr2q4qh 43 21 , , , cord-320015-lbr2q4qh 43 22 delayed delay VBN cord-320015-lbr2q4qh 43 23 early early JJ cord-320015-lbr2q4qh 43 24 ( ( -LRB- cord-320015-lbr2q4qh 43 25 DE DE NNP cord-320015-lbr2q4qh 43 26 ) ) -RRB- cord-320015-lbr2q4qh 43 27 , , , cord-320015-lbr2q4qh 43 28 and and CC cord-320015-lbr2q4qh 43 29 late late JJ cord-320015-lbr2q4qh 43 30 ( ( -LRB- cord-320015-lbr2q4qh 43 31 L l NN cord-320015-lbr2q4qh 43 32 ) ) -RRB- cord-320015-lbr2q4qh 43 33 genes gene NNS cord-320015-lbr2q4qh 43 34 ) ) -RRB- cord-320015-lbr2q4qh 43 35 nucleotide nucleotide JJ cord-320015-lbr2q4qh 43 36 sequences sequence NNS cord-320015-lbr2q4qh 43 37 corresponding correspond VBG cord-320015-lbr2q4qh 43 38 to to IN cord-320015-lbr2q4qh 43 39 IE IE NNP cord-320015-lbr2q4qh 43 40 , , , cord-320015-lbr2q4qh 43 41 DE DE NNP cord-320015-lbr2q4qh 43 42 , , , cord-320015-lbr2q4qh 43 43 and and CC cord-320015-lbr2q4qh 43 44 L l NN cord-320015-lbr2q4qh 43 45 promoters promoter NNS cord-320015-lbr2q4qh 43 46 have have VBP cord-320015-lbr2q4qh 43 47 not not RB cord-320015-lbr2q4qh 43 48 yet yet RB cord-320015-lbr2q4qh 43 49 been be VBN cord-320015-lbr2q4qh 43 50 identified identify VBN cord-320015-lbr2q4qh 43 51 . . . cord-320015-lbr2q4qh 44 1 The the DT cord-320015-lbr2q4qh 44 2 observation observation NN cord-320015-lbr2q4qh 44 3 that that IN cord-320015-lbr2q4qh 44 4 gene gene NN cord-320015-lbr2q4qh 44 5 order order NN cord-320015-lbr2q4qh 44 6 is be VBZ cord-320015-lbr2q4qh 44 7 not not RB cord-320015-lbr2q4qh 44 8 conserved conserve VBN cord-320015-lbr2q4qh 44 9 supports support NNS cord-320015-lbr2q4qh 44 10 the the DT cord-320015-lbr2q4qh 44 11 view view NN cord-320015-lbr2q4qh 44 12 that that IN cord-320015-lbr2q4qh 44 13 genes gene NNS cord-320015-lbr2q4qh 44 14 can can MD cord-320015-lbr2q4qh 44 15 be be VB cord-320015-lbr2q4qh 44 16 reshuffled reshuffle VBN cord-320015-lbr2q4qh 44 17 through through IN cord-320015-lbr2q4qh 44 18 recombination recombination NN cord-320015-lbr2q4qh 44 19 without without IN cord-320015-lbr2q4qh 44 20 adversely adversely RB cord-320015-lbr2q4qh 44 21 affecting affect VBG cord-320015-lbr2q4qh 44 22 expression expression NN cord-320015-lbr2q4qh 44 23 . . . cord-320015-lbr2q4qh 45 1 Microarray Microarray NNP cord-320015-lbr2q4qh 45 2 analysis analysis NN cord-320015-lbr2q4qh 45 3 suggests suggest VBZ cord-320015-lbr2q4qh 45 4 that that IN cord-320015-lbr2q4qh 45 5 FV3 FV3 NNP cord-320015-lbr2q4qh 45 6 contains contain VBZ cord-320015-lbr2q4qh 45 7 33 33 CD cord-320015-lbr2q4qh 45 8 IE IE NNP cord-320015-lbr2q4qh 45 9 , , , cord-320015-lbr2q4qh 45 10 22 22 CD cord-320015-lbr2q4qh 45 11 DE de NN cord-320015-lbr2q4qh 45 12 , , , cord-320015-lbr2q4qh 45 13 and and CC cord-320015-lbr2q4qh 45 14 36 36 CD cord-320015-lbr2q4qh 45 15 L l NN cord-320015-lbr2q4qh 45 16 genes gene NNS cord-320015-lbr2q4qh 45 17 [ [ -LRB- cord-320015-lbr2q4qh 45 18 22 22 CD cord-320015-lbr2q4qh 45 19 ] ] -RRB- cord-320015-lbr2q4qh 45 20 . . . cord-320015-lbr2q4qh 46 1 Moreover moreover RB cord-320015-lbr2q4qh 46 2 , , , cord-320015-lbr2q4qh 46 3 the the DT cord-320015-lbr2q4qh 46 4 classes class NNS cord-320015-lbr2q4qh 46 5 assigned assign VBN cord-320015-lbr2q4qh 46 6 in in IN cord-320015-lbr2q4qh 46 7 that that DT cord-320015-lbr2q4qh 46 8 study study NN cord-320015-lbr2q4qh 46 9 were be VBD cord-320015-lbr2q4qh 46 10 generally generally RB cord-320015-lbr2q4qh 46 11 similar similar JJ cord-320015-lbr2q4qh 46 12 to to IN cord-320015-lbr2q4qh 46 13 those those DT cord-320015-lbr2q4qh 46 14 assigned assign VBN cord-320015-lbr2q4qh 46 15 to to IN cord-320015-lbr2q4qh 46 16 known know VBN cord-320015-lbr2q4qh 46 17 SGIV SGIV NNP cord-320015-lbr2q4qh 46 18 genes gene NNS cord-320015-lbr2q4qh 46 19 in in IN cord-320015-lbr2q4qh 46 20 two two CD cord-320015-lbr2q4qh 46 21 earlier early JJR cord-320015-lbr2q4qh 46 22 studies study NNS cord-320015-lbr2q4qh 46 23 [ [ -LRB- cord-320015-lbr2q4qh 46 24 23 23 CD cord-320015-lbr2q4qh 46 25 , , , cord-320015-lbr2q4qh 46 26 24 24 CD cord-320015-lbr2q4qh 46 27 ] ] -RRB- cord-320015-lbr2q4qh 46 28 . . . cord-320015-lbr2q4qh 47 1 Although although IN cord-320015-lbr2q4qh 47 2 all all DT cord-320015-lbr2q4qh 47 3 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 47 4 contain contain VBP cord-320015-lbr2q4qh 47 5 a a DT cord-320015-lbr2q4qh 47 6 core core NN cord-320015-lbr2q4qh 47 7 set set NN cord-320015-lbr2q4qh 47 8 of of IN cord-320015-lbr2q4qh 47 9 72 72 CD cord-320015-lbr2q4qh 47 10 genes gene NNS cord-320015-lbr2q4qh 47 11 [ [ -LRB- cord-320015-lbr2q4qh 47 12 11 11 CD cord-320015-lbr2q4qh 47 13 ] ] -RRB- cord-320015-lbr2q4qh 47 14 , , , cord-320015-lbr2q4qh 47 15 unique unique JJ cord-320015-lbr2q4qh 47 16 genes gene NNS cord-320015-lbr2q4qh 47 17 are be VBP cord-320015-lbr2q4qh 47 18 found find VBN cord-320015-lbr2q4qh 47 19 within within IN cord-320015-lbr2q4qh 47 20 specific specific JJ cord-320015-lbr2q4qh 47 21 viral viral JJ cord-320015-lbr2q4qh 47 22 species specie NNS cord-320015-lbr2q4qh 47 23 . . . cord-320015-lbr2q4qh 48 1 Genes gene NNS cord-320015-lbr2q4qh 48 2 held hold VBN cord-320015-lbr2q4qh 48 3 in in IN cord-320015-lbr2q4qh 48 4 common common JJ cord-320015-lbr2q4qh 48 5 ( ( -LRB- cord-320015-lbr2q4qh 48 6 e.g. e.g. RB cord-320015-lbr2q4qh 48 7 , , , cord-320015-lbr2q4qh 48 8 viral viral JJ cord-320015-lbr2q4qh 48 9 DNA dna NN cord-320015-lbr2q4qh 48 10 polymerase polymerase NN cord-320015-lbr2q4qh 48 11 , , , cord-320015-lbr2q4qh 48 12 MCP MCP NNP cord-320015-lbr2q4qh 48 13 , , , cord-320015-lbr2q4qh 48 14 the the DT cord-320015-lbr2q4qh 48 15 large large JJ cord-320015-lbr2q4qh 48 16 and and CC cord-320015-lbr2q4qh 48 17 small small JJ cord-320015-lbr2q4qh 48 18 subunits subunit NNS cord-320015-lbr2q4qh 48 19 of of IN cord-320015-lbr2q4qh 48 20 the the DT cord-320015-lbr2q4qh 48 21 viral viral JJ cord-320015-lbr2q4qh 48 22 RNA RNA NNP cord-320015-lbr2q4qh 48 23 transcriptase transcriptase NN cord-320015-lbr2q4qh 48 24 , , , cord-320015-lbr2q4qh 48 25 etc etc FW cord-320015-lbr2q4qh 48 26 . . . cord-320015-lbr2q4qh 48 27 ) ) -RRB- cord-320015-lbr2q4qh 48 28 are be VBP cord-320015-lbr2q4qh 48 29 thought think VBN cord-320015-lbr2q4qh 48 30 to to TO cord-320015-lbr2q4qh 48 31 be be VB cord-320015-lbr2q4qh 48 32 those those DT cord-320015-lbr2q4qh 48 33 required require VBN cord-320015-lbr2q4qh 48 34 for for IN cord-320015-lbr2q4qh 48 35 replication replication NN cord-320015-lbr2q4qh 48 36 in in IN cord-320015-lbr2q4qh 48 37 all all DT cord-320015-lbr2q4qh 48 38 cell cell NN cord-320015-lbr2q4qh 48 39 types type NNS cord-320015-lbr2q4qh 48 40 , , , cord-320015-lbr2q4qh 48 41 whereas whereas IN cord-320015-lbr2q4qh 48 42 those those DT cord-320015-lbr2q4qh 48 43 unique unique JJ cord-320015-lbr2q4qh 48 44 to to IN cord-320015-lbr2q4qh 48 45 a a DT cord-320015-lbr2q4qh 48 46 given give VBN cord-320015-lbr2q4qh 48 47 viral viral JJ cord-320015-lbr2q4qh 48 48 species specie NNS cord-320015-lbr2q4qh 48 49 may may MD cord-320015-lbr2q4qh 48 50 represent represent VB cord-320015-lbr2q4qh 48 51 specific specific JJ cord-320015-lbr2q4qh 48 52 host host NN cord-320015-lbr2q4qh 48 53 adaptations adaptation NNS cord-320015-lbr2q4qh 48 54 that that WDT cord-320015-lbr2q4qh 48 55 contribute contribute VBP cord-320015-lbr2q4qh 48 56 to to IN cord-320015-lbr2q4qh 48 57 virulence virulence NN cord-320015-lbr2q4qh 48 58 , , , cord-320015-lbr2q4qh 48 59 host host NN cord-320015-lbr2q4qh 48 60 range range NN cord-320015-lbr2q4qh 48 61 , , , cord-320015-lbr2q4qh 48 62 and and CC cord-320015-lbr2q4qh 48 63 immune immune JJ cord-320015-lbr2q4qh 48 64 evasion evasion NN cord-320015-lbr2q4qh 48 65 . . . cord-320015-lbr2q4qh 49 1 Early early JJ cord-320015-lbr2q4qh 49 2 studies study NNS cord-320015-lbr2q4qh 49 3 of of IN cord-320015-lbr2q4qh 49 4 iridovirid iridovirid NNP cord-320015-lbr2q4qh 49 5 replication replication NN cord-320015-lbr2q4qh 49 6 were be VBD cord-320015-lbr2q4qh 49 7 conducted conduct VBN cord-320015-lbr2q4qh 49 8 almost almost RB cord-320015-lbr2q4qh 49 9 exclusively exclusively RB cord-320015-lbr2q4qh 49 10 using use VBG cord-320015-lbr2q4qh 49 11 FV3 FV3 NNP cord-320015-lbr2q4qh 49 12 , , , cord-320015-lbr2q4qh 49 13 whereas whereas IN cord-320015-lbr2q4qh 49 14 recent recent JJ cord-320015-lbr2q4qh 49 15 studies study NNS cord-320015-lbr2q4qh 49 16 have have VBP cord-320015-lbr2q4qh 49 17 included include VBN cord-320015-lbr2q4qh 49 18 additional additional JJ cord-320015-lbr2q4qh 49 19 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 49 20 such such JJ cord-320015-lbr2q4qh 49 21 as as IN cord-320015-lbr2q4qh 49 22 ATV ATV NNP cord-320015-lbr2q4qh 49 23 and and CC cord-320015-lbr2q4qh 49 24 SGIV SGIV NNP cord-320015-lbr2q4qh 49 25 and and CC cord-320015-lbr2q4qh 49 26 several several JJ cord-320015-lbr2q4qh 49 27 megalocytivirus megalocytivirus NN cord-320015-lbr2q4qh 49 28 isolates isolate VBZ cord-320015-lbr2q4qh 49 29 that that WDT cord-320015-lbr2q4qh 49 30 have have VBP cord-320015-lbr2q4qh 49 31 been be VBN cord-320015-lbr2q4qh 49 32 linked link VBN cord-320015-lbr2q4qh 49 33 to to IN cord-320015-lbr2q4qh 49 34 massive massive JJ cord-320015-lbr2q4qh 49 35 die die NN cord-320015-lbr2q4qh 49 36 - - HYPH cord-320015-lbr2q4qh 49 37 offs off NNS cord-320015-lbr2q4qh 49 38 in in IN cord-320015-lbr2q4qh 49 39 mariculture mariculture JJ cord-320015-lbr2q4qh 49 40 facilities facility NNS cord-320015-lbr2q4qh 49 41 in in IN cord-320015-lbr2q4qh 49 42 Japan Japan NNP cord-320015-lbr2q4qh 49 43 and and CC cord-320015-lbr2q4qh 49 44 south south NNP cord-320015-lbr2q4qh 49 45 - - HYPH cord-320015-lbr2q4qh 49 46 east east NN cord-320015-lbr2q4qh 49 47 Asia Asia NNP cord-320015-lbr2q4qh 49 48 . . . cord-320015-lbr2q4qh 50 1 Below below IN cord-320015-lbr2q4qh 50 2 we -PRON- PRP cord-320015-lbr2q4qh 50 3 summarize summarize VBP cord-320015-lbr2q4qh 50 4 events event NNS cord-320015-lbr2q4qh 50 5 in in IN cord-320015-lbr2q4qh 50 6 FV3-infected fv3-infected JJ cord-320015-lbr2q4qh 50 7 cells cell NNS cord-320015-lbr2q4qh 50 8 and and CC cord-320015-lbr2q4qh 50 9 highlight highlight VBP cord-320015-lbr2q4qh 50 10 recent recent JJ cord-320015-lbr2q4qh 50 11 work work NN cord-320015-lbr2q4qh 50 12 that that WDT cord-320015-lbr2q4qh 50 13 has have VBZ cord-320015-lbr2q4qh 50 14 appeared appear VBN cord-320015-lbr2q4qh 50 15 since since IN cord-320015-lbr2q4qh 50 16 the the DT cord-320015-lbr2q4qh 50 17 last last JJ cord-320015-lbr2q4qh 50 18 comprehensive comprehensive JJ cord-320015-lbr2q4qh 50 19 reviews review NNS cord-320015-lbr2q4qh 50 20 [ [ -LRB- cord-320015-lbr2q4qh 50 21 1 1 CD cord-320015-lbr2q4qh 50 22 , , , cord-320015-lbr2q4qh 50 23 2 2 CD cord-320015-lbr2q4qh 50 24 , , , cord-320015-lbr2q4qh 50 25 15 15 CD cord-320015-lbr2q4qh 50 26 , , , cord-320015-lbr2q4qh 50 27 25 25 CD cord-320015-lbr2q4qh 50 28 ] ] -RRB- cord-320015-lbr2q4qh 50 29 . . . cord-320015-lbr2q4qh 51 1 FV3 FV3 NNP cord-320015-lbr2q4qh 51 2 virions virion NNS cord-320015-lbr2q4qh 51 3 , , , cord-320015-lbr2q4qh 51 4 and and CC cord-320015-lbr2q4qh 51 5 presumably presumably RB cord-320015-lbr2q4qh 51 6 virions virion NNS cord-320015-lbr2q4qh 51 7 of of IN cord-320015-lbr2q4qh 51 8 all all DT cord-320015-lbr2q4qh 51 9 other other JJ cord-320015-lbr2q4qh 51 10 iridoviruses iridoviruse NNS cord-320015-lbr2q4qh 51 11 , , , cord-320015-lbr2q4qh 51 12 exist exist VBP cord-320015-lbr2q4qh 51 13 in in IN cord-320015-lbr2q4qh 51 14 two two CD cord-320015-lbr2q4qh 51 15 forms form NNS cord-320015-lbr2q4qh 51 16 : : : cord-320015-lbr2q4qh 51 17 non non JJ cord-320015-lbr2q4qh 51 18 - - JJ cord-320015-lbr2q4qh 51 19 enveloped enveloped JJ cord-320015-lbr2q4qh 51 20 ( ( -LRB- cord-320015-lbr2q4qh 51 21 i.e. i.e. FW cord-320015-lbr2q4qh 51 22 , , , cord-320015-lbr2q4qh 51 23 naked naked JJ cord-320015-lbr2q4qh 51 24 ) ) -RRB- cord-320015-lbr2q4qh 51 25 particles particle NNS cord-320015-lbr2q4qh 51 26 and and CC cord-320015-lbr2q4qh 51 27 enveloped envelop VBN cord-320015-lbr2q4qh 51 28 virions virion NNS cord-320015-lbr2q4qh 51 29 . . . cord-320015-lbr2q4qh 52 1 Although although IN cord-320015-lbr2q4qh 52 2 both both DT cord-320015-lbr2q4qh 52 3 forms form NNS cord-320015-lbr2q4qh 52 4 are be VBP cord-320015-lbr2q4qh 52 5 infectious infectious JJ cord-320015-lbr2q4qh 52 6 , , , cord-320015-lbr2q4qh 52 7 enveloped enveloped JJ cord-320015-lbr2q4qh 52 8 virions virion NNS cord-320015-lbr2q4qh 52 9 were be VBD cord-320015-lbr2q4qh 52 10 shown show VBN cord-320015-lbr2q4qh 52 11 to to TO cord-320015-lbr2q4qh 52 12 have have VB cord-320015-lbr2q4qh 52 13 higher high JJR cord-320015-lbr2q4qh 52 14 specific specific JJ cord-320015-lbr2q4qh 52 15 infectivity infectivity NN cord-320015-lbr2q4qh 52 16 [ [ -LRB- cord-320015-lbr2q4qh 52 17 26 26 CD cord-320015-lbr2q4qh 52 18 , , , cord-320015-lbr2q4qh 52 19 27 27 CD cord-320015-lbr2q4qh 52 20 ] ] -RRB- cord-320015-lbr2q4qh 52 21 . . . cord-320015-lbr2q4qh 53 1 With with IN cord-320015-lbr2q4qh 53 2 regard regard NN cord-320015-lbr2q4qh 53 3 to to IN cord-320015-lbr2q4qh 53 4 entry entry NN cord-320015-lbr2q4qh 53 5 , , , cord-320015-lbr2q4qh 53 6 enveloped envelop VBD cord-320015-lbr2q4qh 53 7 particles particle NNS cord-320015-lbr2q4qh 53 8 likely likely RB cord-320015-lbr2q4qh 53 9 enter enter VBP cord-320015-lbr2q4qh 53 10 cells cell NNS cord-320015-lbr2q4qh 53 11 by by IN cord-320015-lbr2q4qh 53 12 receptor receptor NN cord-320015-lbr2q4qh 53 13 - - HYPH cord-320015-lbr2q4qh 53 14 mediated mediate VBN cord-320015-lbr2q4qh 53 15 endocytosis endocytosis NN cord-320015-lbr2q4qh 53 16 in in IN cord-320015-lbr2q4qh 53 17 a a DT cord-320015-lbr2q4qh 53 18 pH ph JJ cord-320015-lbr2q4qh 53 19 - - HYPH cord-320015-lbr2q4qh 53 20 dependent dependent JJ cord-320015-lbr2q4qh 53 21 manner manner NN cord-320015-lbr2q4qh 53 22 and and CC cord-320015-lbr2q4qh 53 23 require require VBP cord-320015-lbr2q4qh 53 24 clathrin clathrin NN cord-320015-lbr2q4qh 53 25 - - HYPH cord-320015-lbr2q4qh 53 26 coated coat VBN cord-320015-lbr2q4qh 53 27 pits pit NNS cord-320015-lbr2q4qh 53 28 , , , cord-320015-lbr2q4qh 53 29 whereas whereas IN cord-320015-lbr2q4qh 53 30 naked naked JJ cord-320015-lbr2q4qh 53 31 particles particle NNS cord-320015-lbr2q4qh 53 32 enter enter VBP cord-320015-lbr2q4qh 53 33 by by IN cord-320015-lbr2q4qh 53 34 fusion fusion NN cord-320015-lbr2q4qh 53 35 at at IN cord-320015-lbr2q4qh 53 36 the the DT cord-320015-lbr2q4qh 53 37 plasma plasma NN cord-320015-lbr2q4qh 53 38 membrane membrane NN cord-320015-lbr2q4qh 53 39 [ [ -LRB- cord-320015-lbr2q4qh 53 40 26 26 CD cord-320015-lbr2q4qh 53 41 , , , cord-320015-lbr2q4qh 53 42 28 28 CD cord-320015-lbr2q4qh 53 43 ] ] -RRB- cord-320015-lbr2q4qh 53 44 . . . cord-320015-lbr2q4qh 54 1 Recently recently RB cord-320015-lbr2q4qh 54 2 this this DT cord-320015-lbr2q4qh 54 3 model model NN cord-320015-lbr2q4qh 54 4 has have VBZ cord-320015-lbr2q4qh 54 5 been be VBN cord-320015-lbr2q4qh 54 6 questioned question VBN cord-320015-lbr2q4qh 54 7 by by IN cord-320015-lbr2q4qh 54 8 Guo Guo NNP cord-320015-lbr2q4qh 54 9 and and CC cord-320015-lbr2q4qh 54 10 co co NNS cord-320015-lbr2q4qh 54 11 - - NNS cord-320015-lbr2q4qh 54 12 workers worker NNS cord-320015-lbr2q4qh 54 13 who who WP cord-320015-lbr2q4qh 54 14 argued argue VBD cord-320015-lbr2q4qh 54 15 that that IN cord-320015-lbr2q4qh 54 16 tiger tiger NNP cord-320015-lbr2q4qh 54 17 frog frog NNP cord-320015-lbr2q4qh 54 18 virus virus NN cord-320015-lbr2q4qh 54 19 ( ( -LRB- cord-320015-lbr2q4qh 54 20 TFV TFV NNP cord-320015-lbr2q4qh 54 21 ) ) -RRB- cord-320015-lbr2q4qh 54 22 , , , cord-320015-lbr2q4qh 54 23 a a DT cord-320015-lbr2q4qh 54 24 ranavirus ranavirus NN cord-320015-lbr2q4qh 54 25 nearly nearly RB cord-320015-lbr2q4qh 54 26 identical identical JJ cord-320015-lbr2q4qh 54 27 to to IN cord-320015-lbr2q4qh 54 28 FV3 FV3 NNP cord-320015-lbr2q4qh 54 29 , , , cord-320015-lbr2q4qh 54 30 enters enter VBZ cord-320015-lbr2q4qh 54 31 cells cell NNS cord-320015-lbr2q4qh 54 32 by by IN cord-320015-lbr2q4qh 54 33 an an DT cord-320015-lbr2q4qh 54 34 atypical atypical JJ cord-320015-lbr2q4qh 54 35 , , , cord-320015-lbr2q4qh 54 36 pH ph NN cord-320015-lbr2q4qh 54 37 - - HYPH cord-320015-lbr2q4qh 54 38 dependent dependent JJ cord-320015-lbr2q4qh 54 39 , , , cord-320015-lbr2q4qh 54 40 caveola caveola JJ cord-320015-lbr2q4qh 54 41 - - HYPH cord-320015-lbr2q4qh 54 42 mediated mediate VBN cord-320015-lbr2q4qh 54 43 endocytic endocytic JJ cord-320015-lbr2q4qh 54 44 pathway pathway NN cord-320015-lbr2q4qh 54 45 [ [ -LRB- cord-320015-lbr2q4qh 54 46 29 29 CD cord-320015-lbr2q4qh 54 47 ] ] -RRB- cord-320015-lbr2q4qh 54 48 . . . cord-320015-lbr2q4qh 55 1 Their -PRON- PRP$ cord-320015-lbr2q4qh 55 2 conclusion conclusion NN cord-320015-lbr2q4qh 55 3 was be VBD cord-320015-lbr2q4qh 55 4 based base VBN cord-320015-lbr2q4qh 55 5 on on IN cord-320015-lbr2q4qh 55 6 experiments experiment NNS cord-320015-lbr2q4qh 55 7 using use VBG cord-320015-lbr2q4qh 55 8 chlorpromazine chlorpromazine NN cord-320015-lbr2q4qh 55 9 and and CC cord-320015-lbr2q4qh 55 10 over over IN cord-320015-lbr2q4qh 55 11 - - HYPH cord-320015-lbr2q4qh 55 12 expression expression NN cord-320015-lbr2q4qh 55 13 of of IN cord-320015-lbr2q4qh 55 14 a a DT cord-320015-lbr2q4qh 55 15 dominant dominant JJ cord-320015-lbr2q4qh 55 16 - - HYPH cord-320015-lbr2q4qh 55 17 negative negative JJ cord-320015-lbr2q4qh 55 18 form form NN cord-320015-lbr2q4qh 55 19 of of IN cord-320015-lbr2q4qh 55 20 Esp15 Esp15 NNP cord-320015-lbr2q4qh 55 21 that that IN cord-320015-lbr2q4qh 55 22 inhibited inhibit VBN cord-320015-lbr2q4qh 55 23 assembly assembly NN cord-320015-lbr2q4qh 55 24 of of IN cord-320015-lbr2q4qh 55 25 clathrin clathrin NN cord-320015-lbr2q4qh 55 26 - - HYPH cord-320015-lbr2q4qh 55 27 coated coat VBN cord-320015-lbr2q4qh 55 28 pits pit NNS cord-320015-lbr2q4qh 55 29 , , , cord-320015-lbr2q4qh 55 30 but but CC cord-320015-lbr2q4qh 55 31 did do VBD cord-320015-lbr2q4qh 55 32 not not RB cord-320015-lbr2q4qh 55 33 affect affect VB cord-320015-lbr2q4qh 55 34 entry entry NN cord-320015-lbr2q4qh 55 35 . . . cord-320015-lbr2q4qh 56 1 Consistent consistent JJ cord-320015-lbr2q4qh 56 2 with with IN cord-320015-lbr2q4qh 56 3 a a DT cord-320015-lbr2q4qh 56 4 role role NN cord-320015-lbr2q4qh 56 5 for for IN cord-320015-lbr2q4qh 56 6 caveolae caveolae NNP cord-320015-lbr2q4qh 56 7 , , , cord-320015-lbr2q4qh 56 8 endocytosis endocytosis NN cord-320015-lbr2q4qh 56 9 of of IN cord-320015-lbr2q4qh 56 10 TFV TFV NNP cord-320015-lbr2q4qh 56 11 was be VBD cord-320015-lbr2q4qh 56 12 dependent dependent JJ cord-320015-lbr2q4qh 56 13 on on IN cord-320015-lbr2q4qh 56 14 membrane membrane NN cord-320015-lbr2q4qh 56 15 cholesterol cholesterol NN cord-320015-lbr2q4qh 56 16 and and CC cord-320015-lbr2q4qh 56 17 was be VBD cord-320015-lbr2q4qh 56 18 blocked block VBN cord-320015-lbr2q4qh 56 19 by by IN cord-320015-lbr2q4qh 56 20 caveolin-1 caveolin-1 NNP cord-320015-lbr2q4qh 56 21 scaffolding scaffold VBG cord-320015-lbr2q4qh 56 22 domain domain NN cord-320015-lbr2q4qh 56 23 protein protein NN cord-320015-lbr2q4qh 56 24 . . . cord-320015-lbr2q4qh 57 1 Given give VBN cord-320015-lbr2q4qh 57 2 that that DT cord-320015-lbr2q4qh 57 3 FV3 FV3 NNP cord-320015-lbr2q4qh 57 4 and and CC cord-320015-lbr2q4qh 57 5 TFV TFV NNP cord-320015-lbr2q4qh 57 6 are be VBP cord-320015-lbr2q4qh 57 7 nearly nearly RB cord-320015-lbr2q4qh 57 8 identical identical JJ cord-320015-lbr2q4qh 57 9 in in IN cord-320015-lbr2q4qh 57 10 nucleotide nucleotide JJ cord-320015-lbr2q4qh 57 11 sequence sequence NN cord-320015-lbr2q4qh 57 12 [ [ -LRB- cord-320015-lbr2q4qh 57 13 30 30 CD cord-320015-lbr2q4qh 57 14 , , , cord-320015-lbr2q4qh 57 15 31 31 CD cord-320015-lbr2q4qh 57 16 ] ] -RRB- cord-320015-lbr2q4qh 57 17 , , , cord-320015-lbr2q4qh 57 18 it -PRON- PRP cord-320015-lbr2q4qh 57 19 is be VBZ cord-320015-lbr2q4qh 57 20 surprising surprising JJ cord-320015-lbr2q4qh 57 21 that that IN cord-320015-lbr2q4qh 57 22 their -PRON- PRP$ cord-320015-lbr2q4qh 57 23 modes mode NNS cord-320015-lbr2q4qh 57 24 of of IN cord-320015-lbr2q4qh 57 25 entry entry NN cord-320015-lbr2q4qh 57 26 are be VBP cord-320015-lbr2q4qh 57 27 so so RB cord-320015-lbr2q4qh 57 28 different different JJ cord-320015-lbr2q4qh 57 29 . . . cord-320015-lbr2q4qh 58 1 Because because IN cord-320015-lbr2q4qh 58 2 the the DT cord-320015-lbr2q4qh 58 3 reported report VBN cord-320015-lbr2q4qh 58 4 differences difference NNS cord-320015-lbr2q4qh 58 5 in in IN cord-320015-lbr2q4qh 58 6 entry entry NN cord-320015-lbr2q4qh 58 7 mechanisms mechanism NNS cord-320015-lbr2q4qh 58 8 between between IN cord-320015-lbr2q4qh 58 9 FV3 FV3 NNP cord-320015-lbr2q4qh 58 10 and and CC cord-320015-lbr2q4qh 58 11 TFV TFV NNP cord-320015-lbr2q4qh 58 12 may may MD cord-320015-lbr2q4qh 58 13 be be VB cord-320015-lbr2q4qh 58 14 due due JJ cord-320015-lbr2q4qh 58 15 to to IN cord-320015-lbr2q4qh 58 16 infection infection NN cord-320015-lbr2q4qh 58 17 of of IN cord-320015-lbr2q4qh 58 18 cells cell NNS cord-320015-lbr2q4qh 58 19 from from IN cord-320015-lbr2q4qh 58 20 different different JJ cord-320015-lbr2q4qh 58 21 non non JJ cord-320015-lbr2q4qh 58 22 - - JJ cord-320015-lbr2q4qh 58 23 physiological physiological JJ cord-320015-lbr2q4qh 58 24 hosts host NNS cord-320015-lbr2q4qh 58 25 , , , cord-320015-lbr2q4qh 58 26 e.g. e.g. RB cord-320015-lbr2q4qh 58 27 , , , cord-320015-lbr2q4qh 58 28 rats rat NNS cord-320015-lbr2q4qh 58 29 and and CC cord-320015-lbr2q4qh 58 30 hamsters hamster NNS cord-320015-lbr2q4qh 58 31 , , , cord-320015-lbr2q4qh 58 32 resolution resolution NN cord-320015-lbr2q4qh 58 33 of of IN cord-320015-lbr2q4qh 58 34 this this DT cord-320015-lbr2q4qh 58 35 issue issue NN cord-320015-lbr2q4qh 58 36 will will MD cord-320015-lbr2q4qh 58 37 require require VB cord-320015-lbr2q4qh 58 38 direct direct JJ cord-320015-lbr2q4qh 58 39 comparison comparison NN cord-320015-lbr2q4qh 58 40 of of IN cord-320015-lbr2q4qh 58 41 TFV TFV NNP cord-320015-lbr2q4qh 58 42 and and CC cord-320015-lbr2q4qh 58 43 FV3 FV3 NNP cord-320015-lbr2q4qh 58 44 entry entry NN cord-320015-lbr2q4qh 58 45 using use VBG cord-320015-lbr2q4qh 58 46 the the DT cord-320015-lbr2q4qh 58 47 above above JJ cord-320015-lbr2q4qh 58 48 approaches approach NNS cord-320015-lbr2q4qh 58 49 coupled couple VBN cord-320015-lbr2q4qh 58 50 with with IN cord-320015-lbr2q4qh 58 51 TEM TEM NNP cord-320015-lbr2q4qh 58 52 . . . cord-320015-lbr2q4qh 59 1 Regardless regardless RB cord-320015-lbr2q4qh 59 2 of of IN cord-320015-lbr2q4qh 59 3 whether whether IN cord-320015-lbr2q4qh 59 4 uncoating uncoating NN cord-320015-lbr2q4qh 59 5 takes take VBZ cord-320015-lbr2q4qh 59 6 place place NN cord-320015-lbr2q4qh 59 7 at at IN cord-320015-lbr2q4qh 59 8 the the DT cord-320015-lbr2q4qh 59 9 plasma plasma NN cord-320015-lbr2q4qh 59 10 or or CC cord-320015-lbr2q4qh 59 11 nuclear nuclear JJ cord-320015-lbr2q4qh 59 12 membranes membrane NNS cord-320015-lbr2q4qh 59 13 , , , cord-320015-lbr2q4qh 59 14 the the DT cord-320015-lbr2q4qh 59 15 viral viral JJ cord-320015-lbr2q4qh 59 16 genome genome NN cord-320015-lbr2q4qh 59 17 enters enter VBZ cord-320015-lbr2q4qh 59 18 the the DT cord-320015-lbr2q4qh 59 19 nucleus nucleus NN cord-320015-lbr2q4qh 59 20 . . . cord-320015-lbr2q4qh 60 1 Unlike unlike IN cord-320015-lbr2q4qh 60 2 herpesviruses herpesviruses NNP cord-320015-lbr2q4qh 60 3 , , , cord-320015-lbr2q4qh 60 4 iridovirid iridovirid NNP cord-320015-lbr2q4qh 60 5 genomes genome NNS cord-320015-lbr2q4qh 60 6 are be VBP cord-320015-lbr2q4qh 60 7 not not RB cord-320015-lbr2q4qh 60 8 infectious infectious JJ cord-320015-lbr2q4qh 60 9 , , , cord-320015-lbr2q4qh 60 10 indicating indicate VBG cord-320015-lbr2q4qh 60 11 that that IN cord-320015-lbr2q4qh 60 12 virion virion NN cord-320015-lbr2q4qh 60 13 - - HYPH cord-320015-lbr2q4qh 60 14 associated associate VBN cord-320015-lbr2q4qh 60 15 proteins protein NNS cord-320015-lbr2q4qh 60 16 are be VBP cord-320015-lbr2q4qh 60 17 required require VBN cord-320015-lbr2q4qh 60 18 to to TO cord-320015-lbr2q4qh 60 19 initiate initiate VB cord-320015-lbr2q4qh 60 20 viral viral JJ cord-320015-lbr2q4qh 60 21 gene gene NN cord-320015-lbr2q4qh 60 22 transcription transcription NN cord-320015-lbr2q4qh 60 23 [ [ -LRB- cord-320015-lbr2q4qh 60 24 32 32 CD cord-320015-lbr2q4qh 60 25 ] ] -RRB- cord-320015-lbr2q4qh 60 26 . . . cord-320015-lbr2q4qh 61 1 Accordingly accordingly RB cord-320015-lbr2q4qh 61 2 , , , cord-320015-lbr2q4qh 61 3 immediate immediate JJ cord-320015-lbr2q4qh 61 4 - - HYPH cord-320015-lbr2q4qh 61 5 early early JJ cord-320015-lbr2q4qh 61 6 ( ( -LRB- cord-320015-lbr2q4qh 61 7 IE IE NNP cord-320015-lbr2q4qh 61 8 ) ) -RRB- cord-320015-lbr2q4qh 61 9 and and CC cord-320015-lbr2q4qh 61 10 delayed delay VBD cord-320015-lbr2q4qh 61 11 early early RB cord-320015-lbr2q4qh 62 1 ( ( -LRB- cord-320015-lbr2q4qh 62 2 DE DE NNP cord-320015-lbr2q4qh 62 3 ) ) -RRB- cord-320015-lbr2q4qh 62 4 viral viral JJ cord-320015-lbr2q4qh 62 5 transcripts transcript NNS cord-320015-lbr2q4qh 62 6 are be VBP cord-320015-lbr2q4qh 62 7 synthesized synthesize VBN cord-320015-lbr2q4qh 62 8 in in IN cord-320015-lbr2q4qh 62 9 reactions reaction NNS cord-320015-lbr2q4qh 62 10 mediated mediate VBN cord-320015-lbr2q4qh 62 11 by by IN cord-320015-lbr2q4qh 62 12 putative putative JJ cord-320015-lbr2q4qh 62 13 virion virion NN cord-320015-lbr2q4qh 62 14 - - HYPH cord-320015-lbr2q4qh 62 15 associated associate VBN cord-320015-lbr2q4qh 62 16 ( ( -LRB- cord-320015-lbr2q4qh 62 17 for for IN cord-320015-lbr2q4qh 62 18 IE IE NNP cord-320015-lbr2q4qh 62 19 transcripts transcript NNS cord-320015-lbr2q4qh 62 20 ) ) -RRB- cord-320015-lbr2q4qh 62 21 and and CC cord-320015-lbr2q4qh 62 22 virus virus NN cord-320015-lbr2q4qh 62 23 - - HYPH cord-320015-lbr2q4qh 62 24 encoded encode VBN cord-320015-lbr2q4qh 62 25 ( ( -LRB- cord-320015-lbr2q4qh 62 26 for for IN cord-320015-lbr2q4qh 62 27 DE DE NNP cord-320015-lbr2q4qh 62 28 mRNA mrna NN cord-320015-lbr2q4qh 62 29 ) ) -RRB- cord-320015-lbr2q4qh 63 1 transcriptional transcriptional NNP cord-320015-lbr2q4qh 63 2 trans trans NNP cord-320015-lbr2q4qh 63 3 - - HYPH cord-320015-lbr2q4qh 63 4 activators activator NNS cord-320015-lbr2q4qh 63 5 and and CC cord-320015-lbr2q4qh 63 6 , , , cord-320015-lbr2q4qh 63 7 at at IN cord-320015-lbr2q4qh 63 8 least least JJS cord-320015-lbr2q4qh 63 9 for for IN cord-320015-lbr2q4qh 63 10 IE IE NNP cord-320015-lbr2q4qh 63 11 transcription transcription NN cord-320015-lbr2q4qh 63 12 , , , cord-320015-lbr2q4qh 63 13 host host NN cord-320015-lbr2q4qh 63 14 RNA rna NN cord-320015-lbr2q4qh 63 15 polymerase polymerase NN cord-320015-lbr2q4qh 63 16 II II NNP cord-320015-lbr2q4qh 63 17 [ [ -LRB- cord-320015-lbr2q4qh 63 18 33 33 CD cord-320015-lbr2q4qh 63 19 ] ] -RRB- cord-320015-lbr2q4qh 64 1 [ [ -LRB- cord-320015-lbr2q4qh 64 2 34 34 CD cord-320015-lbr2q4qh 64 3 ] ] -RRB- cord-320015-lbr2q4qh 64 4 [ [ -LRB- cord-320015-lbr2q4qh 64 5 35 35 CD cord-320015-lbr2q4qh 64 6 ] ] -RRB- cord-320015-lbr2q4qh 64 7 . . . cord-320015-lbr2q4qh 65 1 Among among IN cord-320015-lbr2q4qh 65 2 the the DT cord-320015-lbr2q4qh 65 3 products product NNS cord-320015-lbr2q4qh 65 4 of of IN cord-320015-lbr2q4qh 65 5 early early JJ cord-320015-lbr2q4qh 65 6 transcription transcription NN cord-320015-lbr2q4qh 65 7 is be VBZ cord-320015-lbr2q4qh 65 8 a a DT cord-320015-lbr2q4qh 65 9 viral viral JJ cord-320015-lbr2q4qh 65 10 DNA dna NN cord-320015-lbr2q4qh 65 11 polymerase polymerase NN cord-320015-lbr2q4qh 65 12 which which WDT cord-320015-lbr2q4qh 65 13 catalyzes catalyze VBZ cord-320015-lbr2q4qh 65 14 the the DT cord-320015-lbr2q4qh 65 15 synthesis synthesis NN cord-320015-lbr2q4qh 65 16 of of IN cord-320015-lbr2q4qh 65 17 unit unit NN cord-320015-lbr2q4qh 65 18 - - HYPH cord-320015-lbr2q4qh 65 19 sized sized JJ cord-320015-lbr2q4qh 65 20 copies copy NNS cord-320015-lbr2q4qh 65 21 of of IN cord-320015-lbr2q4qh 65 22 the the DT cord-320015-lbr2q4qh 65 23 viral viral JJ cord-320015-lbr2q4qh 65 24 genome genome NN cord-320015-lbr2q4qh 65 25 within within IN cord-320015-lbr2q4qh 65 26 the the DT cord-320015-lbr2q4qh 65 27 nucleus nucleus NN cord-320015-lbr2q4qh 65 28 [ [ -LRB- cord-320015-lbr2q4qh 65 29 36 36 CD cord-320015-lbr2q4qh 65 30 ] ] -RRB- cord-320015-lbr2q4qh 65 31 . . . cord-320015-lbr2q4qh 66 1 Additional additional JJ cord-320015-lbr2q4qh 66 2 IE IE NNP cord-320015-lbr2q4qh 66 3 and and CC cord-320015-lbr2q4qh 66 4 DE DE NNP cord-320015-lbr2q4qh 66 5 proteins protein NNS cord-320015-lbr2q4qh 66 6 include include VBP cord-320015-lbr2q4qh 66 7 proteins protein NNS cord-320015-lbr2q4qh 66 8 that that WDT cord-320015-lbr2q4qh 66 9 may may MD cord-320015-lbr2q4qh 66 10 play play VB cord-320015-lbr2q4qh 66 11 roles role NNS cord-320015-lbr2q4qh 66 12 in in IN cord-320015-lbr2q4qh 66 13 blocking block VBG cord-320015-lbr2q4qh 66 14 host host NN cord-320015-lbr2q4qh 66 15 immune immune JJ cord-320015-lbr2q4qh 66 16 responses response NNS cord-320015-lbr2q4qh 66 17 such such JJ cord-320015-lbr2q4qh 66 18 as as IN cord-320015-lbr2q4qh 66 19 a a DT cord-320015-lbr2q4qh 66 20 virus virus NN cord-320015-lbr2q4qh 66 21 - - HYPH cord-320015-lbr2q4qh 66 22 encoded encode VBN cord-320015-lbr2q4qh 66 23 , , , cord-320015-lbr2q4qh 66 24 CARD card NN cord-320015-lbr2q4qh 66 25 ( ( -LRB- cord-320015-lbr2q4qh 66 26 caspase caspase NN cord-320015-lbr2q4qh 66 27 activation activation NN cord-320015-lbr2q4qh 66 28 and and CC cord-320015-lbr2q4qh 66 29 recruitment recruitment NN cord-320015-lbr2q4qh 66 30 domain domain NN cord-320015-lbr2q4qh 66 31 ) ) -RRB- cord-320015-lbr2q4qh 66 32 motif motif NN cord-320015-lbr2q4qh 66 33 - - HYPH cord-320015-lbr2q4qh 66 34 containing contain VBG cord-320015-lbr2q4qh 66 35 protein protein NN cord-320015-lbr2q4qh 66 36 ( ( -LRB- cord-320015-lbr2q4qh 66 37 vCARD vcard RB cord-320015-lbr2q4qh 66 38 ) ) -RRB- cord-320015-lbr2q4qh 66 39 , , , cord-320015-lbr2q4qh 66 40 β β NN cord-320015-lbr2q4qh 66 41 - - HYPH cord-320015-lbr2q4qh 66 42 hydroxysteroid hydroxysteroid JJ cord-320015-lbr2q4qh 66 43 dehydrogenase dehydrogenase NN cord-320015-lbr2q4qh 66 44 ( ( -LRB- cord-320015-lbr2q4qh 66 45 βHSD βHSD NNP cord-320015-lbr2q4qh 66 46 ) ) -RRB- cord-320015-lbr2q4qh 66 47 , , , cord-320015-lbr2q4qh 66 48 and and CC cord-320015-lbr2q4qh 66 49 a a DT cord-320015-lbr2q4qh 67 1 RNAse rnase UH cord-320015-lbr2q4qh 67 2 III III NNP cord-320015-lbr2q4qh 67 3 - - HYPH cord-320015-lbr2q4qh 67 4 like like JJ cord-320015-lbr2q4qh 67 5 protein protein NN cord-320015-lbr2q4qh 67 6 , , , cord-320015-lbr2q4qh 67 7 catalytic catalytic JJ cord-320015-lbr2q4qh 67 8 proteins protein NNS cord-320015-lbr2q4qh 67 9 involved involve VBN cord-320015-lbr2q4qh 67 10 in in IN cord-320015-lbr2q4qh 67 11 nucleic nucleic JJ cord-320015-lbr2q4qh 67 12 acid acid NN cord-320015-lbr2q4qh 67 13 synthesis synthesis NN cord-320015-lbr2q4qh 68 1 ( ( -LRB- cord-320015-lbr2q4qh 68 2 Proliferating proliferate VBG cord-320015-lbr2q4qh 68 3 Cell Cell NNP cord-320015-lbr2q4qh 68 4 Nuclear Nuclear NNP cord-320015-lbr2q4qh 68 5 Antigen Antigen NNP cord-320015-lbr2q4qh 68 6 [ [ -LRB- cord-320015-lbr2q4qh 68 7 PCNA PCNA NNP cord-320015-lbr2q4qh 68 8 ] ] -RRB- cord-320015-lbr2q4qh 68 9 , , . cord-320015-lbr2q4qh 69 1 DNA dna NN cord-320015-lbr2q4qh 69 2 methyltransferase methyltransferase NN cord-320015-lbr2q4qh 69 3 [ [ -LRB- cord-320015-lbr2q4qh 69 4 DMTase DMTase NNP cord-320015-lbr2q4qh 69 5 ] ] -RRB- cord-320015-lbr2q4qh 69 6 , , , cord-320015-lbr2q4qh 69 7 the the DT cord-320015-lbr2q4qh 69 8 large large JJ cord-320015-lbr2q4qh 69 9 and and CC cord-320015-lbr2q4qh 69 10 small small JJ cord-320015-lbr2q4qh 69 11 subunits subunit NNS cord-320015-lbr2q4qh 69 12 of of IN cord-320015-lbr2q4qh 69 13 the the DT cord-320015-lbr2q4qh 69 14 viral viral JJ cord-320015-lbr2q4qh 69 15 homolog homolog NN cord-320015-lbr2q4qh 69 16 of of IN cord-320015-lbr2q4qh 69 17 cellular cellular JJ cord-320015-lbr2q4qh 69 18 RNA RNA NNP cord-320015-lbr2q4qh 69 19 polymerase polymerase NN cord-320015-lbr2q4qh 69 20 II II NNP cord-320015-lbr2q4qh 69 21 [ [ -LRB- cord-320015-lbr2q4qh 69 22 vPOL vPOL VBN cord-320015-lbr2q4qh 69 23 - - JJ cord-320015-lbr2q4qh 69 24 IIα iiα JJ cord-320015-lbr2q4qh 69 25 and and CC cord-320015-lbr2q4qh 69 26 -IIβ -IIβ , cord-320015-lbr2q4qh 69 27 ] ] -RRB- cord-320015-lbr2q4qh 69 28 , , , cord-320015-lbr2q4qh 69 29 transcription transcription NN cord-320015-lbr2q4qh 69 30 factor factor NN cord-320015-lbr2q4qh 69 31 IIS IIS NNP cord-320015-lbr2q4qh 69 32 ) ) -RRB- cord-320015-lbr2q4qh 69 33 , , , cord-320015-lbr2q4qh 69 34 catalytic catalytic JJ cord-320015-lbr2q4qh 69 35 proteins protein NNS cord-320015-lbr2q4qh 69 36 that that WDT cord-320015-lbr2q4qh 69 37 may may MD cord-320015-lbr2q4qh 69 38 act act VB cord-320015-lbr2q4qh 69 39 to to TO cord-320015-lbr2q4qh 69 40 increase increase VB cord-320015-lbr2q4qh 69 41 dTTP dttp JJ cord-320015-lbr2q4qh 69 42 pool pool NN cord-320015-lbr2q4qh 69 43 sizes size NNS cord-320015-lbr2q4qh 69 44 and and CC cord-320015-lbr2q4qh 69 45 influence influence NN cord-320015-lbr2q4qh 69 46 host host NN cord-320015-lbr2q4qh 69 47 range range NN cord-320015-lbr2q4qh 69 48 ( ( -LRB- cord-320015-lbr2q4qh 69 49 deoxyuridine deoxyuridine NN cord-320015-lbr2q4qh 69 50 triphosphatase triphosphatase NN cord-320015-lbr2q4qh 69 51 [ [ -LRB- cord-320015-lbr2q4qh 69 52 dUTPase dUTPase '' cord-320015-lbr2q4qh 69 53 ] ] -RRB- cord-320015-lbr2q4qh 69 54 , , . cord-320015-lbr2q4qh 70 1 deoxynucleotide deoxynucleotide NNP cord-320015-lbr2q4qh 70 2 kinase kinase NNP cord-320015-lbr2q4qh 70 3 , , , cord-320015-lbr2q4qh 70 4 the the DT cord-320015-lbr2q4qh 70 5 large large JJ cord-320015-lbr2q4qh 70 6 and and CC cord-320015-lbr2q4qh 70 7 small small JJ cord-320015-lbr2q4qh 70 8 subunits subunit NNS cord-320015-lbr2q4qh 70 9 of of IN cord-320015-lbr2q4qh 70 10 ribonucleotide ribonucleotide NN cord-320015-lbr2q4qh 70 11 reductase reductase NN cord-320015-lbr2q4qh 70 12 ) ) -RRB- cord-320015-lbr2q4qh 70 13 , , , cord-320015-lbr2q4qh 70 14 and and CC cord-320015-lbr2q4qh 70 15 proteins protein NNS cord-320015-lbr2q4qh 70 16 non non JJ cord-320015-lbr2q4qh 70 17 - - JJ cord-320015-lbr2q4qh 70 18 essential essential JJ cord-320015-lbr2q4qh 70 19 for for IN cord-320015-lbr2q4qh 70 20 replication replication NN cord-320015-lbr2q4qh 70 21 in in IN cord-320015-lbr2q4qh 70 22 vitro vitro FW cord-320015-lbr2q4qh 70 23 , , , cord-320015-lbr2q4qh 70 24 but but CC cord-320015-lbr2q4qh 70 25 needed need VBN cord-320015-lbr2q4qh 70 26 for for IN cord-320015-lbr2q4qh 70 27 growth growth NN cord-320015-lbr2q4qh 70 28 in in IN cord-320015-lbr2q4qh 70 29 vivo vivo NNP cord-320015-lbr2q4qh 70 30 ( ( -LRB- cord-320015-lbr2q4qh 70 31 the the DT cord-320015-lbr2q4qh 70 32 18 18 CD cord-320015-lbr2q4qh 70 33 K K NNP cord-320015-lbr2q4qh 70 34 protein protein NN cord-320015-lbr2q4qh 70 35 ) ) -RRB- cord-320015-lbr2q4qh 70 36 [ [ -LRB- cord-320015-lbr2q4qh 70 37 22 22 CD cord-320015-lbr2q4qh 70 38 ] ] -RRB- cord-320015-lbr2q4qh 70 39 . . . cord-320015-lbr2q4qh 71 1 Following follow VBG cord-320015-lbr2q4qh 71 2 its -PRON- PRP$ cord-320015-lbr2q4qh 71 3 synthesis synthesis NN cord-320015-lbr2q4qh 71 4 , , , cord-320015-lbr2q4qh 71 5 unit unit NN cord-320015-lbr2q4qh 71 6 - - HYPH cord-320015-lbr2q4qh 71 7 sized sized JJ cord-320015-lbr2q4qh 71 8 viral viral JJ cord-320015-lbr2q4qh 71 9 DNA dna NN cord-320015-lbr2q4qh 71 10 is be VBZ cord-320015-lbr2q4qh 71 11 transported transport VBN cord-320015-lbr2q4qh 71 12 into into IN cord-320015-lbr2q4qh 71 13 the the DT cord-320015-lbr2q4qh 71 14 cytoplasm cytoplasm NN cord-320015-lbr2q4qh 71 15 where where WRB cord-320015-lbr2q4qh 71 16 it -PRON- PRP cord-320015-lbr2q4qh 71 17 is be VBZ cord-320015-lbr2q4qh 71 18 methylated methylate VBN cord-320015-lbr2q4qh 71 19 by by IN cord-320015-lbr2q4qh 71 20 a a DT cord-320015-lbr2q4qh 71 21 virus virus NN cord-320015-lbr2q4qh 71 22 - - HYPH cord-320015-lbr2q4qh 71 23 encoded encode VBN cord-320015-lbr2q4qh 71 24 DMTase DMTase NNP cord-320015-lbr2q4qh 71 25 and and CC cord-320015-lbr2q4qh 71 26 , , , cord-320015-lbr2q4qh 71 27 following follow VBG cord-320015-lbr2q4qh 71 28 a a DT cord-320015-lbr2q4qh 71 29 second second JJ cord-320015-lbr2q4qh 71 30 round round NN cord-320015-lbr2q4qh 71 31 of of IN cord-320015-lbr2q4qh 71 32 DNA dna NN cord-320015-lbr2q4qh 71 33 synthesis synthesis NN cord-320015-lbr2q4qh 71 34 , , , cord-320015-lbr2q4qh 71 35 converted convert VBN cord-320015-lbr2q4qh 71 36 into into IN cord-320015-lbr2q4qh 71 37 large large JJ cord-320015-lbr2q4qh 71 38 concatameric concatameric JJ cord-320015-lbr2q4qh 71 39 molecules molecule NNS cord-320015-lbr2q4qh 71 40 that that WDT cord-320015-lbr2q4qh 71 41 are be VBP cord-320015-lbr2q4qh 71 42 thought think VBN cord-320015-lbr2q4qh 71 43 to to TO cord-320015-lbr2q4qh 71 44 be be VB cord-320015-lbr2q4qh 71 45 the the DT cord-320015-lbr2q4qh 71 46 substrate substrate NN cord-320015-lbr2q4qh 71 47 from from IN cord-320015-lbr2q4qh 71 48 which which WDT cord-320015-lbr2q4qh 71 49 viral viral JJ cord-320015-lbr2q4qh 71 50 genomes genome NNS cord-320015-lbr2q4qh 71 51 are be VBP cord-320015-lbr2q4qh 71 52 derived derive VBN cord-320015-lbr2q4qh 71 53 [ [ -LRB- cord-320015-lbr2q4qh 71 54 36 36 CD cord-320015-lbr2q4qh 71 55 ] ] -RRB- cord-320015-lbr2q4qh 72 1 [ [ -LRB- cord-320015-lbr2q4qh 72 2 37 37 CD cord-320015-lbr2q4qh 72 3 ] ] -RRB- cord-320015-lbr2q4qh 72 4 [ [ -LRB- cord-320015-lbr2q4qh 72 5 38 38 CD cord-320015-lbr2q4qh 72 6 ] ] -RRB- cord-320015-lbr2q4qh 72 7 . . . cord-320015-lbr2q4qh 73 1 Virion virion NN cord-320015-lbr2q4qh 73 2 formation formation NN cord-320015-lbr2q4qh 73 3 takes take VBZ cord-320015-lbr2q4qh 73 4 place place NN cord-320015-lbr2q4qh 73 5 in in IN cord-320015-lbr2q4qh 73 6 electron electron NN cord-320015-lbr2q4qh 73 7 - - HYPH cord-320015-lbr2q4qh 73 8 lucent lucent JJ cord-320015-lbr2q4qh 73 9 , , , cord-320015-lbr2q4qh 73 10 morphologically morphologically RB cord-320015-lbr2q4qh 73 11 - - HYPH cord-320015-lbr2q4qh 73 12 distinct distinct JJ cord-320015-lbr2q4qh 73 13 VAS vas NN cord-320015-lbr2q4qh 73 14 . . . cord-320015-lbr2q4qh 74 1 VAS VAS NNP cord-320015-lbr2q4qh 74 2 contain contain VBP cord-320015-lbr2q4qh 74 3 viral viral JJ cord-320015-lbr2q4qh 74 4 DNA dna NN cord-320015-lbr2q4qh 74 5 and and CC cord-320015-lbr2q4qh 74 6 the the DT cord-320015-lbr2q4qh 74 7 structural structural JJ cord-320015-lbr2q4qh 74 8 and and CC cord-320015-lbr2q4qh 74 9 non non JJ cord-320015-lbr2q4qh 74 10 - - JJ cord-320015-lbr2q4qh 74 11 structural structural JJ cord-320015-lbr2q4qh 74 12 proteins protein NNS cord-320015-lbr2q4qh 74 13 that that WDT cord-320015-lbr2q4qh 74 14 give give VBP cord-320015-lbr2q4qh 74 15 rise rise NN cord-320015-lbr2q4qh 74 16 to to IN cord-320015-lbr2q4qh 74 17 virions virion NNS cord-320015-lbr2q4qh 74 18 , , , cord-320015-lbr2q4qh 74 19 but but CC cord-320015-lbr2q4qh 74 20 are be VBP cord-320015-lbr2q4qh 74 21 devoid devoid JJ cord-320015-lbr2q4qh 74 22 of of IN cord-320015-lbr2q4qh 74 23 ribosomes ribosome NNS cord-320015-lbr2q4qh 74 24 , , , cord-320015-lbr2q4qh 74 25 mitochondria mitochondrion NNS cord-320015-lbr2q4qh 74 26 , , , cord-320015-lbr2q4qh 74 27 and and CC cord-320015-lbr2q4qh 74 28 other other JJ cord-320015-lbr2q4qh 74 29 cellular cellular JJ cord-320015-lbr2q4qh 74 30 organelles organelle NNS cord-320015-lbr2q4qh 74 31 [ [ -LRB- cord-320015-lbr2q4qh 74 32 39 39 CD cord-320015-lbr2q4qh 74 33 , , , cord-320015-lbr2q4qh 74 34 40 40 CD cord-320015-lbr2q4qh 74 35 ] ] -RRB- cord-320015-lbr2q4qh 74 36 . . . cord-320015-lbr2q4qh 75 1 Although although IN cord-320015-lbr2q4qh 75 2 little little JJ cord-320015-lbr2q4qh 75 3 is be VBZ cord-320015-lbr2q4qh 75 4 known know VBN cord-320015-lbr2q4qh 75 5 about about IN cord-320015-lbr2q4qh 75 6 the the DT cord-320015-lbr2q4qh 75 7 precise precise JJ cord-320015-lbr2q4qh 75 8 process process NN cord-320015-lbr2q4qh 75 9 of of IN cord-320015-lbr2q4qh 75 10 virion virion NN cord-320015-lbr2q4qh 75 11 morphogenesis morphogenesis NN cord-320015-lbr2q4qh 75 12 , , , cord-320015-lbr2q4qh 75 13 by by IN cord-320015-lbr2q4qh 75 14 analogy analogy NN cord-320015-lbr2q4qh 75 15 to to IN cord-320015-lbr2q4qh 75 16 African african JJ cord-320015-lbr2q4qh 75 17 swine swine NN cord-320015-lbr2q4qh 75 18 fever fever NN cord-320015-lbr2q4qh 75 19 virus virus NN cord-320015-lbr2q4qh 75 20 and and CC cord-320015-lbr2q4qh 75 21 by by IN cord-320015-lbr2q4qh 75 22 study study NN cord-320015-lbr2q4qh 75 23 of of IN cord-320015-lbr2q4qh 75 24 various various JJ cord-320015-lbr2q4qh 75 25 iridovirids iridovirid NNS cord-320015-lbr2q4qh 75 26 , , , cord-320015-lbr2q4qh 75 27 it -PRON- PRP cord-320015-lbr2q4qh 75 28 appears appear VBZ cord-320015-lbr2q4qh 75 29 that that IN cord-320015-lbr2q4qh 75 30 host host NN cord-320015-lbr2q4qh 75 31 membranes membrane NNS cord-320015-lbr2q4qh 75 32 , , , cord-320015-lbr2q4qh 75 33 perhaps perhaps RB cord-320015-lbr2q4qh 75 34 derived derive VBN cord-320015-lbr2q4qh 75 35 from from IN cord-320015-lbr2q4qh 75 36 the the DT cord-320015-lbr2q4qh 75 37 ER ER NNP cord-320015-lbr2q4qh 75 38 , , , cord-320015-lbr2q4qh 75 39 serve serve VB cord-320015-lbr2q4qh 75 40 as as IN cord-320015-lbr2q4qh 75 41 a a DT cord-320015-lbr2q4qh 75 42 scaffold scaffold NN cord-320015-lbr2q4qh 75 43 to to TO cord-320015-lbr2q4qh 75 44 which which WDT cord-320015-lbr2q4qh 75 45 capsid capsid NN cord-320015-lbr2q4qh 75 46 and and CC cord-320015-lbr2q4qh 75 47 shell shell NN cord-320015-lbr2q4qh 75 48 proteins protein NNS cord-320015-lbr2q4qh 75 49 bind bind VBP cord-320015-lbr2q4qh 75 50 [ [ -LRB- cord-320015-lbr2q4qh 75 51 41 41 CD cord-320015-lbr2q4qh 75 52 , , , cord-320015-lbr2q4qh 75 53 42 42 CD cord-320015-lbr2q4qh 75 54 ] ] -RRB- cord-320015-lbr2q4qh 75 55 . . . cord-320015-lbr2q4qh 76 1 As as IN cord-320015-lbr2q4qh 76 2 progressively progressively RB cord-320015-lbr2q4qh 76 3 larger large JJR cord-320015-lbr2q4qh 76 4 amounts amount NNS cord-320015-lbr2q4qh 76 5 of of IN cord-320015-lbr2q4qh 76 6 viral viral JJ cord-320015-lbr2q4qh 76 7 proteins protein NNS cord-320015-lbr2q4qh 76 8 bind bind VBP cord-320015-lbr2q4qh 76 9 the the DT cord-320015-lbr2q4qh 76 10 membrane membrane NN cord-320015-lbr2q4qh 76 11 scaffold scaffold NN cord-320015-lbr2q4qh 76 12 , , , cord-320015-lbr2q4qh 76 13 crescent crescent NN cord-320015-lbr2q4qh 76 14 - - HYPH cord-320015-lbr2q4qh 76 15 shaped shape VBN cord-320015-lbr2q4qh 76 16 structures structure NNS cord-320015-lbr2q4qh 76 17 that that WDT cord-320015-lbr2q4qh 76 18 resemble resemble VBP cord-320015-lbr2q4qh 76 19 icosahedral icosahedral JJ cord-320015-lbr2q4qh 76 20 vertices vertex NNS cord-320015-lbr2q4qh 76 21 are be VBP cord-320015-lbr2q4qh 76 22 formed form VBN cord-320015-lbr2q4qh 76 23 . . . cord-320015-lbr2q4qh 77 1 Ultimately ultimately RB cord-320015-lbr2q4qh 77 2 both both DT cord-320015-lbr2q4qh 77 3 full full JJ cord-320015-lbr2q4qh 77 4 and and CC cord-320015-lbr2q4qh 77 5 empty empty JJ cord-320015-lbr2q4qh 77 6 icosahedral icosahedral JJ cord-320015-lbr2q4qh 77 7 virus virus NN cord-320015-lbr2q4qh 77 8 - - HYPH cord-320015-lbr2q4qh 77 9 like like JJ cord-320015-lbr2q4qh 77 10 particles particle NNS cord-320015-lbr2q4qh 77 11 are be VBP cord-320015-lbr2q4qh 77 12 detected detect VBN cord-320015-lbr2q4qh 77 13 . . . cord-320015-lbr2q4qh 78 1 However however RB cord-320015-lbr2q4qh 78 2 , , , cord-320015-lbr2q4qh 78 3 it -PRON- PRP cord-320015-lbr2q4qh 78 4 is be VBZ cord-320015-lbr2q4qh 78 5 unclear unclear JJ cord-320015-lbr2q4qh 78 6 precisely precisely RB cord-320015-lbr2q4qh 78 7 how how WRB cord-320015-lbr2q4qh 78 8 the the DT cord-320015-lbr2q4qh 78 9 genome genome NN cord-320015-lbr2q4qh 78 10 is be VBZ cord-320015-lbr2q4qh 78 11 encapsidated encapsidate VBN cord-320015-lbr2q4qh 78 12 . . . cord-320015-lbr2q4qh 79 1 While while IN cord-320015-lbr2q4qh 79 2 packaging package VBG cord-320015-lbr2q4qh 79 3 via via IN cord-320015-lbr2q4qh 79 4 a a DT cord-320015-lbr2q4qh 79 5 headful headful JJ cord-320015-lbr2q4qh 79 6 mechanism mechanism NN cord-320015-lbr2q4qh 79 7 explains explain VBZ cord-320015-lbr2q4qh 79 8 the the DT cord-320015-lbr2q4qh 79 9 circularly circularly RB cord-320015-lbr2q4qh 79 10 - - HYPH cord-320015-lbr2q4qh 79 11 permuted permute VBN cord-320015-lbr2q4qh 79 12 terminal terminal NN cord-320015-lbr2q4qh 79 13 redundancy redundancy NN cord-320015-lbr2q4qh 79 14 detected detect VBN cord-320015-lbr2q4qh 79 15 within within IN cord-320015-lbr2q4qh 79 16 all all DT cord-320015-lbr2q4qh 79 17 iridovirids iridovirid NNS cord-320015-lbr2q4qh 79 18 [ [ -LRB- cord-320015-lbr2q4qh 79 19 43 43 CD cord-320015-lbr2q4qh 79 20 ] ] -RRB- cord-320015-lbr2q4qh 79 21 , , , cord-320015-lbr2q4qh 79 22 it -PRON- PRP cord-320015-lbr2q4qh 79 23 is be VBZ cord-320015-lbr2q4qh 79 24 not not RB cord-320015-lbr2q4qh 79 25 known know VBN cord-320015-lbr2q4qh 79 26 whether whether IN cord-320015-lbr2q4qh 79 27 nearly nearly RB cord-320015-lbr2q4qh 79 28 complete complete JJ cord-320015-lbr2q4qh 79 29 virions virion NNS cord-320015-lbr2q4qh 79 30 bind bind VBP cord-320015-lbr2q4qh 79 31 viral viral JJ cord-320015-lbr2q4qh 79 32 DNA dna NN cord-320015-lbr2q4qh 79 33 and and CC cord-320015-lbr2q4qh 79 34 internalize internalize VB cord-320015-lbr2q4qh 79 35 it -PRON- PRP cord-320015-lbr2q4qh 79 36 through through IN cord-320015-lbr2q4qh 79 37 a a DT cord-320015-lbr2q4qh 79 38 virion virion NN cord-320015-lbr2q4qh 79 39 portal portal NN cord-320015-lbr2q4qh 79 40 as as IN cord-320015-lbr2q4qh 79 41 seen see VBN cord-320015-lbr2q4qh 79 42 in in IN cord-320015-lbr2q4qh 79 43 some some DT cord-320015-lbr2q4qh 79 44 viral viral JJ cord-320015-lbr2q4qh 79 45 systems system NNS cord-320015-lbr2q4qh 79 46 [ [ -LRB- cord-320015-lbr2q4qh 79 47 44 44 CD cord-320015-lbr2q4qh 79 48 ] ] -RRB- cord-320015-lbr2q4qh 80 1 [ [ -LRB- cord-320015-lbr2q4qh 80 2 45 45 CD cord-320015-lbr2q4qh 80 3 ] ] -RRB- cord-320015-lbr2q4qh 80 4 [ [ -LRB- cord-320015-lbr2q4qh 80 5 46 46 CD cord-320015-lbr2q4qh 80 6 ] ] -RRB- cord-320015-lbr2q4qh 80 7 , , , cord-320015-lbr2q4qh 80 8 or or CC cord-320015-lbr2q4qh 80 9 whether whether IN cord-320015-lbr2q4qh 80 10 developing develop VBG cord-320015-lbr2q4qh 80 11 crescents crescent NNS cord-320015-lbr2q4qh 80 12 / / SYM cord-320015-lbr2q4qh 80 13 icosahedrons icosahedron NNS cord-320015-lbr2q4qh 80 14 engulf engulf VBP cord-320015-lbr2q4qh 80 15 the the DT cord-320015-lbr2q4qh 80 16 viral viral JJ cord-320015-lbr2q4qh 80 17 genome genome NN cord-320015-lbr2q4qh 80 18 as as IN cord-320015-lbr2q4qh 80 19 they -PRON- PRP cord-320015-lbr2q4qh 80 20 assemble assemble VBP cord-320015-lbr2q4qh 80 21 . . . cord-320015-lbr2q4qh 81 1 Following follow VBG cord-320015-lbr2q4qh 81 2 their -PRON- PRP$ cord-320015-lbr2q4qh 81 3 formation formation NN cord-320015-lbr2q4qh 81 4 , , , cord-320015-lbr2q4qh 81 5 virions virion NNS cord-320015-lbr2q4qh 81 6 remain remain VBP cord-320015-lbr2q4qh 81 7 within within IN cord-320015-lbr2q4qh 81 8 VAS VAS NNP cord-320015-lbr2q4qh 81 9 , , , cord-320015-lbr2q4qh 81 10 accumulate accumulate VBP cord-320015-lbr2q4qh 81 11 within within IN cord-320015-lbr2q4qh 81 12 cytoplasmic cytoplasmic JJ cord-320015-lbr2q4qh 81 13 paracrystalline paracrystalline NN cord-320015-lbr2q4qh 81 14 arrays array NNS cord-320015-lbr2q4qh 81 15 , , , cord-320015-lbr2q4qh 81 16 or or CC cord-320015-lbr2q4qh 81 17 bud bud NN cord-320015-lbr2q4qh 81 18 from from IN cord-320015-lbr2q4qh 81 19 the the DT cord-320015-lbr2q4qh 81 20 plasma plasma NN cord-320015-lbr2q4qh 81 21 membrane membrane NN cord-320015-lbr2q4qh 81 22 . . . cord-320015-lbr2q4qh 82 1 At at IN cord-320015-lbr2q4qh 82 2 late late JJ cord-320015-lbr2q4qh 82 3 times time NNS cord-320015-lbr2q4qh 82 4 after after IN cord-320015-lbr2q4qh 82 5 infection infection NN cord-320015-lbr2q4qh 82 6 , , , cord-320015-lbr2q4qh 82 7 virions virion NNS cord-320015-lbr2q4qh 82 8 are be VBP cord-320015-lbr2q4qh 82 9 sometimes sometimes RB cord-320015-lbr2q4qh 82 10 seen see VBN cord-320015-lbr2q4qh 82 11 within within IN cord-320015-lbr2q4qh 82 12 the the DT cord-320015-lbr2q4qh 82 13 nucleus nucleus NN cord-320015-lbr2q4qh 82 14 and and CC cord-320015-lbr2q4qh 82 15 elongated elongated JJ cord-320015-lbr2q4qh 82 16 tubular tubular JJ cord-320015-lbr2q4qh 82 17 structures structure NNS cord-320015-lbr2q4qh 82 18 can can MD cord-320015-lbr2q4qh 82 19 be be VB cord-320015-lbr2q4qh 82 20 detected detect VBN cord-320015-lbr2q4qh 82 21 in in IN cord-320015-lbr2q4qh 82 22 VAS VAS NNP cord-320015-lbr2q4qh 82 23 , , , cord-320015-lbr2q4qh 82 24 but but CC cord-320015-lbr2q4qh 82 25 these these DT cord-320015-lbr2q4qh 82 26 are be VBP cord-320015-lbr2q4qh 82 27 likely likely JJ cord-320015-lbr2q4qh 82 28 artifacts artifact NNS cord-320015-lbr2q4qh 82 29 that that WDT cord-320015-lbr2q4qh 82 30 reflect reflect VBP cord-320015-lbr2q4qh 82 31 breakdown breakdown NN cord-320015-lbr2q4qh 82 32 of of IN cord-320015-lbr2q4qh 82 33 the the DT cord-320015-lbr2q4qh 82 34 nuclear nuclear JJ cord-320015-lbr2q4qh 82 35 membrane membrane NN cord-320015-lbr2q4qh 82 36 and and CC cord-320015-lbr2q4qh 82 37 the the DT cord-320015-lbr2q4qh 82 38 disruption disruption NN cord-320015-lbr2q4qh 82 39 of of IN cord-320015-lbr2q4qh 82 40 the the DT cord-320015-lbr2q4qh 82 41 assembly assembly NN cord-320015-lbr2q4qh 82 42 process process NN cord-320015-lbr2q4qh 82 43 due due IN cord-320015-lbr2q4qh 82 44 to to IN cord-320015-lbr2q4qh 82 45 the the DT cord-320015-lbr2q4qh 82 46 shortage shortage NN cord-320015-lbr2q4qh 82 47 of of IN cord-320015-lbr2q4qh 82 48 key key JJ cord-320015-lbr2q4qh 82 49 structural structural JJ cord-320015-lbr2q4qh 82 50 proteins protein NNS cord-320015-lbr2q4qh 82 51 and and CC cord-320015-lbr2q4qh 82 52 the the DT cord-320015-lbr2q4qh 82 53 dysregulation dysregulation NN cord-320015-lbr2q4qh 82 54 of of IN cord-320015-lbr2q4qh 82 55 cellular cellular JJ cord-320015-lbr2q4qh 82 56 and and CC cord-320015-lbr2q4qh 82 57 viral viral JJ cord-320015-lbr2q4qh 82 58 macromolecular macromolecular JJ cord-320015-lbr2q4qh 82 59 synthesis synthesis NN cord-320015-lbr2q4qh 82 60 . . . cord-320015-lbr2q4qh 83 1 A a DT cord-320015-lbr2q4qh 83 2 transmission transmission NN cord-320015-lbr2q4qh 83 3 electron electron NN cord-320015-lbr2q4qh 83 4 micrograph micrograph NN cord-320015-lbr2q4qh 83 5 of of IN cord-320015-lbr2q4qh 83 6 an an DT cord-320015-lbr2q4qh 83 7 FV3-infected fv3-infected JJ cord-320015-lbr2q4qh 83 8 fathead fathead NN cord-320015-lbr2q4qh 83 9 minnow minnow NN cord-320015-lbr2q4qh 83 10 cell cell NN cord-320015-lbr2q4qh 83 11 is be VBZ cord-320015-lbr2q4qh 83 12 shown show VBN cord-320015-lbr2q4qh 83 13 in in IN cord-320015-lbr2q4qh 83 14 Figure Figure NNP cord-320015-lbr2q4qh 83 15 1A 1a NN cord-320015-lbr2q4qh 83 16 , , , cord-320015-lbr2q4qh 83 17 and and CC cord-320015-lbr2q4qh 83 18 an an DT cord-320015-lbr2q4qh 83 19 enlargement enlargement NN cord-320015-lbr2q4qh 83 20 of of IN cord-320015-lbr2q4qh 83 21 the the DT cord-320015-lbr2q4qh 83 22 VAS VAS NNP cord-320015-lbr2q4qh 83 23 , , , cord-320015-lbr2q4qh 83 24 displaying display VBG cord-320015-lbr2q4qh 83 25 full full JJ cord-320015-lbr2q4qh 83 26 and and CC cord-320015-lbr2q4qh 83 27 empty empty JJ cord-320015-lbr2q4qh 83 28 virions virion NNS cord-320015-lbr2q4qh 83 29 , , , cord-320015-lbr2q4qh 83 30 is be VBZ cord-320015-lbr2q4qh 83 31 shown show VBN cord-320015-lbr2q4qh 83 32 in in IN cord-320015-lbr2q4qh 83 33 Figure figure NN cord-320015-lbr2q4qh 83 34 1B 1b CD cord-320015-lbr2q4qh 83 35 . . . cord-320015-lbr2q4qh 84 1 The the DT cord-320015-lbr2q4qh 84 2 identities identity NNS cord-320015-lbr2q4qh 84 3 of of IN cord-320015-lbr2q4qh 84 4 virus virus NN cord-320015-lbr2q4qh 84 5 - - HYPH cord-320015-lbr2q4qh 84 6 encoded encode VBN cord-320015-lbr2q4qh 84 7 proteins protein NNS cord-320015-lbr2q4qh 84 8 involved involve VBN cord-320015-lbr2q4qh 84 9 in in IN cord-320015-lbr2q4qh 84 10 virion virion NN cord-320015-lbr2q4qh 84 11 assembly assembly NN cord-320015-lbr2q4qh 84 12 remain remain VBP cord-320015-lbr2q4qh 84 13 to to TO cord-320015-lbr2q4qh 84 14 be be VB cord-320015-lbr2q4qh 84 15 determined determine VBN cord-320015-lbr2q4qh 84 16 . . . cord-320015-lbr2q4qh 85 1 Twelve twelve CD cord-320015-lbr2q4qh 85 2 complementation complementation NN cord-320015-lbr2q4qh 85 3 groups group NNS cord-320015-lbr2q4qh 85 4 defective defective JJ cord-320015-lbr2q4qh 85 5 in in IN cord-320015-lbr2q4qh 85 6 the the DT cord-320015-lbr2q4qh 85 7 ability ability NN cord-320015-lbr2q4qh 85 8 to to TO cord-320015-lbr2q4qh 85 9 synthesize synthesize VB cord-320015-lbr2q4qh 85 10 infectious infectious JJ cord-320015-lbr2q4qh 85 11 virions virion NNS cord-320015-lbr2q4qh 85 12 have have VBP cord-320015-lbr2q4qh 85 13 been be VBN cord-320015-lbr2q4qh 85 14 identified identify VBN cord-320015-lbr2q4qh 85 15 through through IN cord-320015-lbr2q4qh 85 16 study study NN cord-320015-lbr2q4qh 85 17 of of IN cord-320015-lbr2q4qh 85 18 temperature temperature NN cord-320015-lbr2q4qh 85 19 - - HYPH cord-320015-lbr2q4qh 85 20 sensitive sensitive JJ cord-320015-lbr2q4qh 85 21 ( ( -LRB- cord-320015-lbr2q4qh 85 22 ts ts NN cord-320015-lbr2q4qh 85 23 ) ) -RRB- cord-320015-lbr2q4qh 85 24 mutants mutant NNS cord-320015-lbr2q4qh 85 25 ( ( -LRB- cord-320015-lbr2q4qh 85 26 see see VB cord-320015-lbr2q4qh 85 27 below below RB cord-320015-lbr2q4qh 85 28 ) ) -RRB- cord-320015-lbr2q4qh 85 29 , , , cord-320015-lbr2q4qh 85 30 but but CC cord-320015-lbr2q4qh 85 31 it -PRON- PRP cord-320015-lbr2q4qh 85 32 is be VBZ cord-320015-lbr2q4qh 85 33 unclear unclear JJ cord-320015-lbr2q4qh 85 34 whether whether IN cord-320015-lbr2q4qh 85 35 the the DT cord-320015-lbr2q4qh 85 36 gene gene NN cord-320015-lbr2q4qh 85 37 products product NNS cord-320015-lbr2q4qh 85 38 encoded encode VBN cord-320015-lbr2q4qh 85 39 by by IN cord-320015-lbr2q4qh 85 40 these these DT cord-320015-lbr2q4qh 85 41 12 12 CD cord-320015-lbr2q4qh 85 42 complementation complementation NN cord-320015-lbr2q4qh 85 43 groups group NNS cord-320015-lbr2q4qh 85 44 represent represent VBP cord-320015-lbr2q4qh 85 45 scaffold scaffold NN cord-320015-lbr2q4qh 85 46 proteins protein NNS cord-320015-lbr2q4qh 85 47 that that WDT cord-320015-lbr2q4qh 85 48 are be VBP cord-320015-lbr2q4qh 85 49 required require VBN cord-320015-lbr2q4qh 85 50 for for IN cord-320015-lbr2q4qh 85 51 virion virion NN cord-320015-lbr2q4qh 85 52 assembly assembly NN cord-320015-lbr2q4qh 85 53 , , , cord-320015-lbr2q4qh 85 54 but but CC cord-320015-lbr2q4qh 85 55 which which WDT cord-320015-lbr2q4qh 85 56 are be VBP cord-320015-lbr2q4qh 85 57 not not RB cord-320015-lbr2q4qh 85 58 incorporated incorporate VBN cord-320015-lbr2q4qh 85 59 into into IN cord-320015-lbr2q4qh 85 60 mature mature JJ cord-320015-lbr2q4qh 85 61 particles particle NNS cord-320015-lbr2q4qh 85 62 , , , cord-320015-lbr2q4qh 85 63 authentic authentic JJ cord-320015-lbr2q4qh 85 64 viral viral JJ cord-320015-lbr2q4qh 85 65 structural structural JJ cord-320015-lbr2q4qh 85 66 proteins protein NNS cord-320015-lbr2q4qh 85 67 , , , cord-320015-lbr2q4qh 85 68 or or CC cord-320015-lbr2q4qh 85 69 virion virion NN cord-320015-lbr2q4qh 85 70 - - HYPH cord-320015-lbr2q4qh 85 71 associated associate VBN cord-320015-lbr2q4qh 85 72 accessory accessory NN cord-320015-lbr2q4qh 85 73 proteins protein NNS cord-320015-lbr2q4qh 85 74 required require VBN cord-320015-lbr2q4qh 85 75 for for IN cord-320015-lbr2q4qh 85 76 viral viral JJ cord-320015-lbr2q4qh 85 77 replication replication NN cord-320015-lbr2q4qh 85 78 [ [ -LRB- cord-320015-lbr2q4qh 85 79 47 47 CD cord-320015-lbr2q4qh 85 80 ] ] -RRB- cord-320015-lbr2q4qh 85 81 . . . cord-320015-lbr2q4qh 86 1 Aside aside RB cord-320015-lbr2q4qh 86 2 from from IN cord-320015-lbr2q4qh 86 3 the the DT cord-320015-lbr2q4qh 86 4 MCP MCP NNP cord-320015-lbr2q4qh 86 5 , , , cord-320015-lbr2q4qh 86 6 only only RB cord-320015-lbr2q4qh 86 7 FV3 FV3 NNP cord-320015-lbr2q4qh 86 8 ORF ORF NNP cord-320015-lbr2q4qh 86 9 53R 53R NNP cord-320015-lbr2q4qh 86 10 , , , cord-320015-lbr2q4qh 86 11 which which WDT cord-320015-lbr2q4qh 86 12 encodes encode VBZ cord-320015-lbr2q4qh 86 13 a a DT cord-320015-lbr2q4qh 86 14 putative putative JJ cord-320015-lbr2q4qh 86 15 myristoylated myristoylate VBN cord-320015-lbr2q4qh 86 16 membrane membrane NN cord-320015-lbr2q4qh 86 17 protein protein NN cord-320015-lbr2q4qh 86 18 , , , cord-320015-lbr2q4qh 86 19 has have VBZ cord-320015-lbr2q4qh 86 20 been be VBN cord-320015-lbr2q4qh 86 21 linked link VBN cord-320015-lbr2q4qh 86 22 to to IN cord-320015-lbr2q4qh 86 23 virion virion NN cord-320015-lbr2q4qh 86 24 assembly assembly NN cord-320015-lbr2q4qh 86 25 . . . cord-320015-lbr2q4qh 87 1 Knock knock VB cord-320015-lbr2q4qh 87 2 down down RP cord-320015-lbr2q4qh 87 3 studies study NNS cord-320015-lbr2q4qh 87 4 using use VBG cord-320015-lbr2q4qh 87 5 either either CC cord-320015-lbr2q4qh 87 6 asMOs asMOs NNP cord-320015-lbr2q4qh 87 7 or or CC cord-320015-lbr2q4qh 87 8 artificial artificial JJ cord-320015-lbr2q4qh 87 9 microRNAs microRNAs NNPS cord-320015-lbr2q4qh 87 10 [ [ -LRB- cord-320015-lbr2q4qh 87 11 20 20 CD cord-320015-lbr2q4qh 87 12 , , , cord-320015-lbr2q4qh 87 13 48 48 CD cord-320015-lbr2q4qh 87 14 ] ] -RRB- cord-320015-lbr2q4qh 87 15 demonstrated demonstrate VBD cord-320015-lbr2q4qh 87 16 that that IN cord-320015-lbr2q4qh 87 17 53R 53r CD cord-320015-lbr2q4qh 87 18 was be VBD cord-320015-lbr2q4qh 87 19 required require VBN cord-320015-lbr2q4qh 87 20 for for IN cord-320015-lbr2q4qh 87 21 virion virion NN cord-320015-lbr2q4qh 87 22 assembly assembly NN cord-320015-lbr2q4qh 87 23 , , , cord-320015-lbr2q4qh 87 24 whereas whereas IN cord-320015-lbr2q4qh 87 25 in in FW cord-320015-lbr2q4qh 87 26 vitro vitro FW cord-320015-lbr2q4qh 87 27 studies study NNS cord-320015-lbr2q4qh 87 28 showed show VBD cord-320015-lbr2q4qh 87 29 53R 53r CD cord-320015-lbr2q4qh 87 30 was be VBD cord-320015-lbr2q4qh 87 31 associated associate VBN cord-320015-lbr2q4qh 87 32 with with IN cord-320015-lbr2q4qh 87 33 virus virus NN cord-320015-lbr2q4qh 87 34 factories factory NNS cord-320015-lbr2q4qh 87 35 and and CC cord-320015-lbr2q4qh 87 36 the the DT cord-320015-lbr2q4qh 87 37 virion virion NN cord-320015-lbr2q4qh 87 38 membrane membrane NN cord-320015-lbr2q4qh 87 39 [ [ -LRB- cord-320015-lbr2q4qh 87 40 49 49 CD cord-320015-lbr2q4qh 87 41 ] ] -RRB- cord-320015-lbr2q4qh 87 42 . . . cord-320015-lbr2q4qh 88 1 By by IN cord-320015-lbr2q4qh 88 2 analogy analogy NN cord-320015-lbr2q4qh 88 3 to to IN cord-320015-lbr2q4qh 88 4 ASFV ASFV NNP cord-320015-lbr2q4qh 88 5 , , , cord-320015-lbr2q4qh 88 6 53R 53r CD cord-320015-lbr2q4qh 88 7 may may MD cord-320015-lbr2q4qh 88 8 play play VB cord-320015-lbr2q4qh 88 9 a a DT cord-320015-lbr2q4qh 88 10 role role NN cord-320015-lbr2q4qh 88 11 in in IN cord-320015-lbr2q4qh 88 12 recruiting recruit VBG cord-320015-lbr2q4qh 88 13 ER er NN cord-320015-lbr2q4qh 88 14 - - HYPH cord-320015-lbr2q4qh 88 15 derived derive VBN cord-320015-lbr2q4qh 88 16 membranes membrane NNS cord-320015-lbr2q4qh 88 17 into into IN cord-320015-lbr2q4qh 88 18 virus virus NN cord-320015-lbr2q4qh 88 19 factories factory NNS cord-320015-lbr2q4qh 88 20 where where WRB cord-320015-lbr2q4qh 88 21 they -PRON- PRP cord-320015-lbr2q4qh 88 22 serve serve VBP cord-320015-lbr2q4qh 88 23 as as IN cord-320015-lbr2q4qh 88 24 precursors precursor NNS cord-320015-lbr2q4qh 88 25 of of IN cord-320015-lbr2q4qh 88 26 the the DT cord-320015-lbr2q4qh 88 27 inner inner JJ cord-320015-lbr2q4qh 88 28 viral viral JJ cord-320015-lbr2q4qh 88 29 lipid lipid NN cord-320015-lbr2q4qh 88 30 membrane membrane NN cord-320015-lbr2q4qh 88 31 and/or and/or CC cord-320015-lbr2q4qh 88 32 act act VB cord-320015-lbr2q4qh 88 33 as as IN cord-320015-lbr2q4qh 88 34 a a DT cord-320015-lbr2q4qh 88 35 scaffold scaffold NN cord-320015-lbr2q4qh 88 36 protein protein NN cord-320015-lbr2q4qh 88 37 . . . cord-320015-lbr2q4qh 89 1 As as IN cord-320015-lbr2q4qh 89 2 with with IN cord-320015-lbr2q4qh 89 3 other other JJ cord-320015-lbr2q4qh 89 4 nuclear nuclear JJ cord-320015-lbr2q4qh 89 5 and and CC cord-320015-lbr2q4qh 89 6 cytoplasmic cytoplasmic JJ cord-320015-lbr2q4qh 89 7 , , , cord-320015-lbr2q4qh 89 8 large large JJ cord-320015-lbr2q4qh 89 9 DNA dna NN cord-320015-lbr2q4qh 89 10 viruses virus NNS cord-320015-lbr2q4qh 89 11 ( ( -LRB- cord-320015-lbr2q4qh 89 12 NCLDV NCLDV NNP cord-320015-lbr2q4qh 89 13 ) ) -RRB- cord-320015-lbr2q4qh 89 14 late late JJ cord-320015-lbr2q4qh 89 15 viral viral JJ cord-320015-lbr2q4qh 89 16 gene gene NN cord-320015-lbr2q4qh 89 17 expression expression NN cord-320015-lbr2q4qh 89 18 is be VBZ cord-320015-lbr2q4qh 89 19 dependent dependent JJ cord-320015-lbr2q4qh 89 20 upon upon IN cord-320015-lbr2q4qh 89 21 full full JJ cord-320015-lbr2q4qh 89 22 viral viral JJ cord-320015-lbr2q4qh 89 23 DNA dna NN cord-320015-lbr2q4qh 89 24 synthesis synthesis NN cord-320015-lbr2q4qh 89 25 , , , cord-320015-lbr2q4qh 89 26 and and CC cord-320015-lbr2q4qh 89 27 is be VBZ cord-320015-lbr2q4qh 89 28 catalyzed catalyze VBN cord-320015-lbr2q4qh 89 29 by by IN cord-320015-lbr2q4qh 89 30 a a DT cord-320015-lbr2q4qh 89 31 virus virus NN cord-320015-lbr2q4qh 89 32 - - HYPH cord-320015-lbr2q4qh 89 33 encoded encode VBN cord-320015-lbr2q4qh 89 34 or or CC cord-320015-lbr2q4qh 89 35 virus virus NN cord-320015-lbr2q4qh 89 36 - - HYPH cord-320015-lbr2q4qh 89 37 modified modify VBN cord-320015-lbr2q4qh 89 38 transcriptase transcriptase NN cord-320015-lbr2q4qh 89 39 [ [ -LRB- cord-320015-lbr2q4qh 89 40 47 47 CD cord-320015-lbr2q4qh 89 41 ] ] -RRB- cord-320015-lbr2q4qh 89 42 . . . cord-320015-lbr2q4qh 90 1 Temperature temperature NN cord-320015-lbr2q4qh 90 2 - - HYPH cord-320015-lbr2q4qh 90 3 sensitive sensitive JJ cord-320015-lbr2q4qh 90 4 mutants mutant NNS cord-320015-lbr2q4qh 90 5 defective defective JJ cord-320015-lbr2q4qh 90 6 in in IN cord-320015-lbr2q4qh 90 7 viral viral JJ cord-320015-lbr2q4qh 90 8 DNA dna NN cord-320015-lbr2q4qh 90 9 synthesis synthesis NN cord-320015-lbr2q4qh 90 10 show show NN cord-320015-lbr2q4qh 90 11 markedly markedly RB cord-320015-lbr2q4qh 90 12 reduced reduce VBN cord-320015-lbr2q4qh 90 13 levels level NNS cord-320015-lbr2q4qh 90 14 of of IN cord-320015-lbr2q4qh 90 15 late late JJ cord-320015-lbr2q4qh 90 16 gene gene NN cord-320015-lbr2q4qh 90 17 expression expression NN cord-320015-lbr2q4qh 90 18 as as IN cord-320015-lbr2q4qh 90 19 do do VBP cord-320015-lbr2q4qh 90 20 cells cell NNS cord-320015-lbr2q4qh 90 21 treated treat VBN cord-320015-lbr2q4qh 90 22 with with IN cord-320015-lbr2q4qh 90 23 drugs drug NNS cord-320015-lbr2q4qh 90 24 ( ( -LRB- cord-320015-lbr2q4qh 90 25 phosphonoacetic phosphonoacetic JJ cord-320015-lbr2q4qh 90 26 acid acid NN cord-320015-lbr2q4qh 90 27 [ [ -LRB- cord-320015-lbr2q4qh 90 28 PAA PAA NNP cord-320015-lbr2q4qh 90 29 ] ] -RRB- cord-320015-lbr2q4qh 90 30 , , , cord-320015-lbr2q4qh 90 31 cytosine cytosine NN cord-320015-lbr2q4qh 90 32 arabinoside arabinoside NN cord-320015-lbr2q4qh 90 33 [ [ -LRB- cord-320015-lbr2q4qh 90 34 araC araC NNP cord-320015-lbr2q4qh 90 35 ] ] -RRB- cord-320015-lbr2q4qh 90 36 ) ) -RRB- cord-320015-lbr2q4qh 90 37 that that WDT cord-320015-lbr2q4qh 90 38 block block NN cord-320015-lbr2q4qh 90 39 DNA dna NN cord-320015-lbr2q4qh 90 40 synthesis synthesis NN cord-320015-lbr2q4qh 90 41 [ [ -LRB- cord-320015-lbr2q4qh 90 42 50 50 CD cord-320015-lbr2q4qh 90 43 , , , cord-320015-lbr2q4qh 90 44 51 51 CD cord-320015-lbr2q4qh 90 45 ] ] -RRB- cord-320015-lbr2q4qh 90 46 . . . cord-320015-lbr2q4qh 91 1 FV3 FV3 NNP cord-320015-lbr2q4qh 91 2 and and CC cord-320015-lbr2q4qh 91 3 other other JJ cord-320015-lbr2q4qh 91 4 iridovirids iridovirid NNS cord-320015-lbr2q4qh 91 5 encode encode VBP cord-320015-lbr2q4qh 91 6 homologs homolog NNS cord-320015-lbr2q4qh 91 7 of of IN cord-320015-lbr2q4qh 91 8 the the DT cord-320015-lbr2q4qh 91 9 two two CD cord-320015-lbr2q4qh 91 10 largest large JJS cord-320015-lbr2q4qh 91 11 subunits subunit NNS cord-320015-lbr2q4qh 91 12 of of IN cord-320015-lbr2q4qh 91 13 RNA RNA NNP cord-320015-lbr2q4qh 91 14 polymerase polymerase NN cord-320015-lbr2q4qh 91 15 II II NNP cord-320015-lbr2q4qh 91 16 and and CC cord-320015-lbr2q4qh 91 17 likely likely RB cord-320015-lbr2q4qh 91 18 use use VB cord-320015-lbr2q4qh 91 19 these these DT cord-320015-lbr2q4qh 91 20 proteins protein NNS cord-320015-lbr2q4qh 91 21 , , , cord-320015-lbr2q4qh 91 22 and and CC cord-320015-lbr2q4qh 91 23 perhaps perhaps RB cord-320015-lbr2q4qh 91 24 others other NNS cord-320015-lbr2q4qh 91 25 , , , cord-320015-lbr2q4qh 91 26 to to TO cord-320015-lbr2q4qh 91 27 catalyze catalyze VB cord-320015-lbr2q4qh 91 28 late late JJ cord-320015-lbr2q4qh 91 29 viral viral JJ cord-320015-lbr2q4qh 91 30 gene gene NN cord-320015-lbr2q4qh 91 31 expression expression NN cord-320015-lbr2q4qh 91 32 [ [ -LRB- cord-320015-lbr2q4qh 91 33 30 30 CD cord-320015-lbr2q4qh 91 34 ] ] -RRB- cord-320015-lbr2q4qh 91 35 . . . cord-320015-lbr2q4qh 92 1 Knock knock VB cord-320015-lbr2q4qh 92 2 down down RP cord-320015-lbr2q4qh 92 3 of of IN cord-320015-lbr2q4qh 92 4 the the DT cord-320015-lbr2q4qh 92 5 expression expression NN cord-320015-lbr2q4qh 92 6 of of IN cord-320015-lbr2q4qh 92 7 the the DT cord-320015-lbr2q4qh 92 8 largest large JJS cord-320015-lbr2q4qh 92 9 subunit subunit NN cord-320015-lbr2q4qh 92 10 of of IN cord-320015-lbr2q4qh 92 11 the the DT cord-320015-lbr2q4qh 92 12 viral viral JJ cord-320015-lbr2q4qh 92 13 homolog homolog NN cord-320015-lbr2q4qh 92 14 of of IN cord-320015-lbr2q4qh 92 15 RNA RNA NNP cord-320015-lbr2q4qh 92 16 polymerase polymerase NN cord-320015-lbr2q4qh 92 17 II II NNP cord-320015-lbr2q4qh 92 18 ( ( -LRB- cord-320015-lbr2q4qh 92 19 i.e. i.e. FW cord-320015-lbr2q4qh 92 20 , , , cord-320015-lbr2q4qh 92 21 vPOL vpol JJ cord-320015-lbr2q4qh 92 22 - - JJ cord-320015-lbr2q4qh 92 23 IIα iiα JJ cord-320015-lbr2q4qh 92 24 ) ) -RRB- cord-320015-lbr2q4qh 92 25 results result VBZ cord-320015-lbr2q4qh 92 26 in in IN cord-320015-lbr2q4qh 92 27 a a DT cord-320015-lbr2q4qh 92 28 marked marked JJ cord-320015-lbr2q4qh 92 29 reduction reduction NN cord-320015-lbr2q4qh 92 30 in in IN cord-320015-lbr2q4qh 92 31 the the DT cord-320015-lbr2q4qh 92 32 synthesis synthesis NN cord-320015-lbr2q4qh 92 33 of of IN cord-320015-lbr2q4qh 92 34 late late JJ cord-320015-lbr2q4qh 92 35 viral viral JJ cord-320015-lbr2q4qh 92 36 proteins protein NNS cord-320015-lbr2q4qh 92 37 and and CC cord-320015-lbr2q4qh 92 38 a a DT cord-320015-lbr2q4qh 92 39 corresponding corresponding JJ cord-320015-lbr2q4qh 92 40 reduction reduction NN cord-320015-lbr2q4qh 92 41 in in IN cord-320015-lbr2q4qh 92 42 virion virion NN cord-320015-lbr2q4qh 92 43 formation formation NN cord-320015-lbr2q4qh 92 44 and and CC cord-320015-lbr2q4qh 92 45 supports support VBZ cord-320015-lbr2q4qh 92 46 the the DT cord-320015-lbr2q4qh 92 47 notion notion NN cord-320015-lbr2q4qh 92 48 that that IN cord-320015-lbr2q4qh 92 49 vPOL vpol JJ cord-320015-lbr2q4qh 92 50 - - JJ cord-320015-lbr2q4qh 92 51 IIα iiα JJ cord-320015-lbr2q4qh 92 52 , , , cord-320015-lbr2q4qh 92 53 and and CC cord-320015-lbr2q4qh 92 54 other other JJ cord-320015-lbr2q4qh 92 55 viral viral JJ cord-320015-lbr2q4qh 92 56 proteins protein NNS cord-320015-lbr2q4qh 92 57 , , , cord-320015-lbr2q4qh 92 58 are be VBP cord-320015-lbr2q4qh 92 59 responsible responsible JJ cord-320015-lbr2q4qh 92 60 for for IN cord-320015-lbr2q4qh 92 61 late late JJ cord-320015-lbr2q4qh 92 62 viral viral JJ cord-320015-lbr2q4qh 92 63 gene gene NN cord-320015-lbr2q4qh 92 64 expression expression NN cord-320015-lbr2q4qh 92 65 [ [ -LRB- cord-320015-lbr2q4qh 92 66 52 52 CD cord-320015-lbr2q4qh 92 67 ] ] -RRB- cord-320015-lbr2q4qh 92 68 . . . cord-320015-lbr2q4qh 93 1 Aside aside RB cord-320015-lbr2q4qh 93 2 from from IN cord-320015-lbr2q4qh 93 3 the the DT cord-320015-lbr2q4qh 93 4 MCP MCP NNP cord-320015-lbr2q4qh 93 5 , , , cord-320015-lbr2q4qh 93 6 other other JJ cord-320015-lbr2q4qh 93 7 late late JJ cord-320015-lbr2q4qh 93 8 proteins protein NNS cord-320015-lbr2q4qh 93 9 tentatively tentatively RB cord-320015-lbr2q4qh 93 10 involved involve VBN cord-320015-lbr2q4qh 93 11 in in IN cord-320015-lbr2q4qh 93 12 DNA dna NN cord-320015-lbr2q4qh 93 13 packaging packaging NN cord-320015-lbr2q4qh 93 14 and and CC cord-320015-lbr2q4qh 93 15 virion virion NN cord-320015-lbr2q4qh 93 16 assembly assembly NN cord-320015-lbr2q4qh 93 17 include include VBP cord-320015-lbr2q4qh 93 18 ORF ORF NNP cord-320015-lbr2q4qh 93 19 1R 1R NNP cord-320015-lbr2q4qh 93 20 , , , cord-320015-lbr2q4qh 93 21 a a DT cord-320015-lbr2q4qh 93 22 putative putative JJ cord-320015-lbr2q4qh 93 23 packaging packaging NN cord-320015-lbr2q4qh 93 24 protein protein NN cord-320015-lbr2q4qh 93 25 , , , cord-320015-lbr2q4qh 93 26 and and CC cord-320015-lbr2q4qh 93 27 EVR1 EVR1 NNP cord-320015-lbr2q4qh 93 28 / / SYM cord-320015-lbr2q4qh 93 29 ALR ALR NNP cord-320015-lbr2q4qh 93 30 , , , cord-320015-lbr2q4qh 93 31 a a DT cord-320015-lbr2q4qh 93 32 protein protein NN cord-320015-lbr2q4qh 93 33 involved involve VBN cord-320015-lbr2q4qh 93 34 in in IN cord-320015-lbr2q4qh 93 35 the the DT cord-320015-lbr2q4qh 93 36 formation formation NN cord-320015-lbr2q4qh 93 37 of of IN cord-320015-lbr2q4qh 93 38 disulfide disulfide JJ cord-320015-lbr2q4qh 93 39 bonds bond NNS cord-320015-lbr2q4qh 93 40 [ [ -LRB- cord-320015-lbr2q4qh 93 41 53 53 CD cord-320015-lbr2q4qh 93 42 ] ] -RRB- cord-320015-lbr2q4qh 93 43 . . . cord-320015-lbr2q4qh 94 1 To to TO cord-320015-lbr2q4qh 94 2 illustrate illustrate VB cord-320015-lbr2q4qh 94 3 the the DT cord-320015-lbr2q4qh 94 4 above above JJ cord-320015-lbr2q4qh 94 5 , , , cord-320015-lbr2q4qh 94 6 a a DT cord-320015-lbr2q4qh 94 7 schematic schematic JJ cord-320015-lbr2q4qh 94 8 diagram diagram NN cord-320015-lbr2q4qh 94 9 of of IN cord-320015-lbr2q4qh 94 10 the the DT cord-320015-lbr2q4qh 94 11 viral viral JJ cord-320015-lbr2q4qh 94 12 replication replication NN cord-320015-lbr2q4qh 94 13 cycle cycle NN cord-320015-lbr2q4qh 94 14 is be VBZ cord-320015-lbr2q4qh 94 15 shown show VBN cord-320015-lbr2q4qh 94 16 in in IN cord-320015-lbr2q4qh 94 17 Figure figure NN cord-320015-lbr2q4qh 94 18 2 2 CD cord-320015-lbr2q4qh 94 19 . . . cord-320015-lbr2q4qh 95 1 [ [ -LRB- cord-320015-lbr2q4qh 95 2 54 54 CD cord-320015-lbr2q4qh 95 3 ] ] -RRB- cord-320015-lbr2q4qh 95 4 . . . cord-320015-lbr2q4qh 96 1 Used use VBN cord-320015-lbr2q4qh 96 2 with with IN cord-320015-lbr2q4qh 96 3 permission permission NN cord-320015-lbr2q4qh 96 4 . . . cord-320015-lbr2q4qh 97 1 As as IN cord-320015-lbr2q4qh 97 2 with with IN cord-320015-lbr2q4qh 97 3 other other JJ cord-320015-lbr2q4qh 97 4 viruses virus NNS cord-320015-lbr2q4qh 97 5 , , , cord-320015-lbr2q4qh 97 6 FV3 FV3 NNP cord-320015-lbr2q4qh 97 7 infection infection NN cord-320015-lbr2q4qh 97 8 markedly markedly RB cord-320015-lbr2q4qh 97 9 inhibits inhibit VBZ cord-320015-lbr2q4qh 97 10 host host NN cord-320015-lbr2q4qh 97 11 macromolecular macromolecular JJ cord-320015-lbr2q4qh 97 12 synthesis synthesis NN cord-320015-lbr2q4qh 97 13 and and CC cord-320015-lbr2q4qh 97 14 triggers trigger VBZ cord-320015-lbr2q4qh 97 15 apoptosis apoptosis NN cord-320015-lbr2q4qh 97 16 [ [ -LRB- cord-320015-lbr2q4qh 97 17 55 55 CD cord-320015-lbr2q4qh 97 18 ] ] -RRB- cord-320015-lbr2q4qh 97 19 [ [ -LRB- cord-320015-lbr2q4qh 97 20 56 56 CD cord-320015-lbr2q4qh 97 21 ] ] -RRB- cord-320015-lbr2q4qh 97 22 [ [ -LRB- cord-320015-lbr2q4qh 97 23 57 57 CD cord-320015-lbr2q4qh 97 24 ] ] -RRB- cord-320015-lbr2q4qh 97 25 [ [ -LRB- cord-320015-lbr2q4qh 97 26 58 58 CD cord-320015-lbr2q4qh 97 27 ] ] -RRB- cord-320015-lbr2q4qh 97 28 . . . cord-320015-lbr2q4qh 98 1 Both both DT cord-320015-lbr2q4qh 98 2 phenomena phenomenon NNS cord-320015-lbr2q4qh 98 3 are be VBP cord-320015-lbr2q4qh 98 4 mediated mediate VBN cord-320015-lbr2q4qh 98 5 by by IN cord-320015-lbr2q4qh 98 6 heat heat NN cord-320015-lbr2q4qh 98 7 - - HYPH cord-320015-lbr2q4qh 98 8 or or CC cord-320015-lbr2q4qh 98 9 UV uv NN cord-320015-lbr2q4qh 98 10 - - HYPH cord-320015-lbr2q4qh 98 11 inactivated inactivate VBN cord-320015-lbr2q4qh 98 12 virus virus NN cord-320015-lbr2q4qh 98 13 indicating indicate VBG cord-320015-lbr2q4qh 98 14 that that IN cord-320015-lbr2q4qh 98 15 the the DT cord-320015-lbr2q4qh 98 16 inhibitory inhibitory JJ cord-320015-lbr2q4qh 98 17 agent agent NN cord-320015-lbr2q4qh 98 18 is be VBZ cord-320015-lbr2q4qh 98 19 a a DT cord-320015-lbr2q4qh 98 20 virion virion NN cord-320015-lbr2q4qh 98 21 - - HYPH cord-320015-lbr2q4qh 98 22 associated associate VBN cord-320015-lbr2q4qh 98 23 protein protein NN cord-320015-lbr2q4qh 98 24 [ [ -LRB- cord-320015-lbr2q4qh 98 25 58 58 CD cord-320015-lbr2q4qh 98 26 , , , cord-320015-lbr2q4qh 98 27 59 59 CD cord-320015-lbr2q4qh 98 28 ] ] -RRB- cord-320015-lbr2q4qh 98 29 . . . cord-320015-lbr2q4qh 99 1 Viral viral JJ cord-320015-lbr2q4qh 99 2 infection infection NN cord-320015-lbr2q4qh 99 3 appears appear VBZ cord-320015-lbr2q4qh 99 4 to to TO cord-320015-lbr2q4qh 99 5 trigger trigger VB cord-320015-lbr2q4qh 99 6 activation activation NN cord-320015-lbr2q4qh 99 7 of of IN cord-320015-lbr2q4qh 99 8 PKR PKR NNP cord-320015-lbr2q4qh 99 9 , , , cord-320015-lbr2q4qh 99 10 a a DT cord-320015-lbr2q4qh 99 11 double double RB cord-320015-lbr2q4qh 99 12 - - HYPH cord-320015-lbr2q4qh 99 13 stranded strand VBN cord-320015-lbr2q4qh 99 14 RNA RNA NNP cord-320015-lbr2q4qh 99 15 - - HYPH cord-320015-lbr2q4qh 99 16 activated activate VBN cord-320015-lbr2q4qh 99 17 protein protein NN cord-320015-lbr2q4qh 99 18 kinase kinase NN cord-320015-lbr2q4qh 99 19 , , , cord-320015-lbr2q4qh 99 20 which which WDT cord-320015-lbr2q4qh 99 21 leads lead VBZ cord-320015-lbr2q4qh 99 22 to to IN cord-320015-lbr2q4qh 99 23 the the DT cord-320015-lbr2q4qh 99 24 phosphorylation phosphorylation NN cord-320015-lbr2q4qh 99 25 of of IN cord-320015-lbr2q4qh 99 26 the the DT cord-320015-lbr2q4qh 99 27 largest large JJS cord-320015-lbr2q4qh 99 28 subunit subunit NN cord-320015-lbr2q4qh 99 29 of of IN cord-320015-lbr2q4qh 99 30 eukaryotic eukaryotic JJ cord-320015-lbr2q4qh 99 31 initiation initiation NN cord-320015-lbr2q4qh 99 32 factor factor NN cord-320015-lbr2q4qh 99 33 2 2 CD cord-320015-lbr2q4qh 99 34 ( ( -LRB- cord-320015-lbr2q4qh 99 35 eIF-2α eIF-2α NNS cord-320015-lbr2q4qh 99 36 ) ) -RRB- cord-320015-lbr2q4qh 99 37 and and CC cord-320015-lbr2q4qh 99 38 the the DT cord-320015-lbr2q4qh 99 39 subsequent subsequent JJ cord-320015-lbr2q4qh 99 40 inhibition inhibition NN cord-320015-lbr2q4qh 99 41 of of IN cord-320015-lbr2q4qh 99 42 protein protein NN cord-320015-lbr2q4qh 99 43 synthesis synthesis NN cord-320015-lbr2q4qh 99 44 [ [ -LRB- cord-320015-lbr2q4qh 99 45 56 56 CD cord-320015-lbr2q4qh 99 46 ] ] -RRB- cord-320015-lbr2q4qh 99 47 . . . cord-320015-lbr2q4qh 100 1 Viral viral JJ cord-320015-lbr2q4qh 100 2 translation translation NN cord-320015-lbr2q4qh 100 3 may may MD cord-320015-lbr2q4qh 100 4 be be VB cord-320015-lbr2q4qh 100 5 spared spare VBN cord-320015-lbr2q4qh 100 6 due due IN cord-320015-lbr2q4qh 100 7 to to IN cord-320015-lbr2q4qh 100 8 the the DT cord-320015-lbr2q4qh 100 9 synthesis synthesis NN cord-320015-lbr2q4qh 100 10 of of IN cord-320015-lbr2q4qh 100 11 large large JJ cord-320015-lbr2q4qh 100 12 amounts amount NNS cord-320015-lbr2q4qh 100 13 of of IN cord-320015-lbr2q4qh 100 14 highly highly RB cord-320015-lbr2q4qh 100 15 efficient efficient JJ cord-320015-lbr2q4qh 100 16 viral viral JJ cord-320015-lbr2q4qh 100 17 transcripts transcript NNS cord-320015-lbr2q4qh 100 18 and and CC cord-320015-lbr2q4qh 100 19 the the DT cord-320015-lbr2q4qh 100 20 presence presence NN cord-320015-lbr2q4qh 100 21 of of IN cord-320015-lbr2q4qh 100 22 a a DT cord-320015-lbr2q4qh 100 23 virus virus NN cord-320015-lbr2q4qh 100 24 - - HYPH cord-320015-lbr2q4qh 100 25 encoded encode VBN cord-320015-lbr2q4qh 100 26 protein protein NN cord-320015-lbr2q4qh 100 27 , , , cord-320015-lbr2q4qh 100 28 e.g. e.g. RB cord-320015-lbr2q4qh 100 29 , , , cord-320015-lbr2q4qh 100 30 a a DT cord-320015-lbr2q4qh 100 31 viral viral JJ cord-320015-lbr2q4qh 100 32 homolog homolog NN cord-320015-lbr2q4qh 100 33 of of IN cord-320015-lbr2q4qh 100 34 eIF-2α eIF-2α NNS cord-320015-lbr2q4qh 100 35 ( ( -LRB- cord-320015-lbr2q4qh 100 36 vIF-2α vIF-2α NNP cord-320015-lbr2q4qh 100 37 ) ) -RRB- cord-320015-lbr2q4qh 100 38 , , , cord-320015-lbr2q4qh 100 39 that that DT cord-320015-lbr2q4qh 100 40 acts act VBZ cord-320015-lbr2q4qh 100 41 as as IN cord-320015-lbr2q4qh 100 42 a a DT cord-320015-lbr2q4qh 100 43 pseudosubstrate pseudosubstrate NN cord-320015-lbr2q4qh 100 44 and and CC cord-320015-lbr2q4qh 100 45 prevents prevent VBZ cord-320015-lbr2q4qh 100 46 phosphorylation phosphorylation NN cord-320015-lbr2q4qh 100 47 / / SYM cord-320015-lbr2q4qh 100 48 inactivation inactivation NN cord-320015-lbr2q4qh 100 49 of of IN cord-320015-lbr2q4qh 100 50 eIF-2α eIF-2α NNS cord-320015-lbr2q4qh 100 51 [ [ -LRB- cord-320015-lbr2q4qh 100 52 57 57 CD cord-320015-lbr2q4qh 100 53 , , , cord-320015-lbr2q4qh 100 54 [ [ -LRB- cord-320015-lbr2q4qh 100 55 60 60 CD cord-320015-lbr2q4qh 100 56 ] ] -RRB- cord-320015-lbr2q4qh 100 57 [ [ -LRB- cord-320015-lbr2q4qh 100 58 61 61 CD cord-320015-lbr2q4qh 100 59 ] ] -RRB- cord-320015-lbr2q4qh 100 60 [ [ -LRB- cord-320015-lbr2q4qh 100 61 62 62 CD cord-320015-lbr2q4qh 100 62 ] ] -RRB- cord-320015-lbr2q4qh 100 63 . . . cord-320015-lbr2q4qh 101 1 Most Most JJS cord-320015-lbr2q4qh 101 2 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 101 3 , , , cord-320015-lbr2q4qh 101 4 with with IN cord-320015-lbr2q4qh 101 5 the the DT cord-320015-lbr2q4qh 101 6 exception exception NN cord-320015-lbr2q4qh 101 7 of of IN cord-320015-lbr2q4qh 101 8 FV3 FV3 NNP cord-320015-lbr2q4qh 101 9 , , , cord-320015-lbr2q4qh 101 10 contain contain VBP cord-320015-lbr2q4qh 101 11 a a DT cord-320015-lbr2q4qh 101 12 full full JJ cord-320015-lbr2q4qh 101 13 - - HYPH cord-320015-lbr2q4qh 101 14 sized sized JJ cord-320015-lbr2q4qh 101 15 vIF-2α vif-2α JJ cord-320015-lbr2q4qh 101 16 gene gene NN cord-320015-lbr2q4qh 101 17 [ [ -LRB- cord-320015-lbr2q4qh 101 18 63 63 CD cord-320015-lbr2q4qh 101 19 , , , cord-320015-lbr2q4qh 101 20 64 64 CD cord-320015-lbr2q4qh 101 21 ] ] -RRB- cord-320015-lbr2q4qh 101 22 . . . cord-320015-lbr2q4qh 102 1 However however RB cord-320015-lbr2q4qh 102 2 , , , cord-320015-lbr2q4qh 102 3 because because IN cord-320015-lbr2q4qh 102 4 the the DT cord-320015-lbr2q4qh 102 5 absence absence NN cord-320015-lbr2q4qh 102 6 of of IN cord-320015-lbr2q4qh 102 7 a a DT cord-320015-lbr2q4qh 102 8 full full JJ cord-320015-lbr2q4qh 102 9 - - HYPH cord-320015-lbr2q4qh 102 10 sized sized JJ cord-320015-lbr2q4qh 102 11 vIF-2α vif-2α JJ cord-320015-lbr2q4qh 102 12 gene gene NN cord-320015-lbr2q4qh 102 13 in in IN cord-320015-lbr2q4qh 102 14 FV3 FV3 NNP cord-320015-lbr2q4qh 102 15 does do VBZ cord-320015-lbr2q4qh 102 16 not not RB cord-320015-lbr2q4qh 102 17 adversely adversely RB cord-320015-lbr2q4qh 102 18 affect affect VB cord-320015-lbr2q4qh 102 19 viral viral JJ cord-320015-lbr2q4qh 102 20 protein protein NN cord-320015-lbr2q4qh 102 21 synthesis synthesis NN cord-320015-lbr2q4qh 102 22 , , , cord-320015-lbr2q4qh 102 23 other other JJ cord-320015-lbr2q4qh 102 24 viral viral JJ cord-320015-lbr2q4qh 102 25 proteins protein NNS cord-320015-lbr2q4qh 102 26 may may MD cord-320015-lbr2q4qh 102 27 also also RB cord-320015-lbr2q4qh 102 28 play play VB cord-320015-lbr2q4qh 102 29 roles role NNS cord-320015-lbr2q4qh 102 30 in in IN cord-320015-lbr2q4qh 102 31 maintaining maintain VBG cord-320015-lbr2q4qh 102 32 viral viral JJ cord-320015-lbr2q4qh 102 33 protein protein NN cord-320015-lbr2q4qh 102 34 synthesis synthesis NN cord-320015-lbr2q4qh 102 35 in in IN cord-320015-lbr2q4qh 102 36 infected infected JJ cord-320015-lbr2q4qh 102 37 cells cell NNS cord-320015-lbr2q4qh 102 38 . . . cord-320015-lbr2q4qh 103 1 Aside aside RB cord-320015-lbr2q4qh 103 2 from from IN cord-320015-lbr2q4qh 103 3 the the DT cord-320015-lbr2q4qh 103 4 aforementioned aforementioned JJ cord-320015-lbr2q4qh 103 5 vIF-2α vif-2α JJ cord-320015-lbr2q4qh 103 6 protein protein NN cord-320015-lbr2q4qh 103 7 , , , cord-320015-lbr2q4qh 103 8 FV3 FV3 NNP cord-320015-lbr2q4qh 103 9 and and CC cord-320015-lbr2q4qh 103 10 other other JJ cord-320015-lbr2q4qh 103 11 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 103 12 encode encode VBP cord-320015-lbr2q4qh 103 13 a a DT cord-320015-lbr2q4qh 103 14 number number NN cord-320015-lbr2q4qh 103 15 of of IN cord-320015-lbr2q4qh 103 16 putative putative JJ cord-320015-lbr2q4qh 103 17 immune immune JJ cord-320015-lbr2q4qh 103 18 evasion evasion NN cord-320015-lbr2q4qh 103 19 gene gene NN cord-320015-lbr2q4qh 103 20 products product NNS cord-320015-lbr2q4qh 103 21 including include VBG cord-320015-lbr2q4qh 103 22 βHSD βhsd XX cord-320015-lbr2q4qh 103 23 , , , cord-320015-lbr2q4qh 103 24 vCARD vcard UH cord-320015-lbr2q4qh 103 25 , , , cord-320015-lbr2q4qh 103 26 and and CC cord-320015-lbr2q4qh 103 27 an an DT cord-320015-lbr2q4qh 103 28 RNAse RNAse NNP cord-320015-lbr2q4qh 103 29 III III NNP cord-320015-lbr2q4qh 103 30 - - HYPH cord-320015-lbr2q4qh 103 31 like like JJ cord-320015-lbr2q4qh 103 32 protein protein NN cord-320015-lbr2q4qh 103 33 . . . cord-320015-lbr2q4qh 104 1 By by IN cord-320015-lbr2q4qh 104 2 analogy analogy NN cord-320015-lbr2q4qh 104 3 to to IN cord-320015-lbr2q4qh 104 4 a a DT cord-320015-lbr2q4qh 104 5 similar similar JJ cord-320015-lbr2q4qh 104 6 protein protein NN cord-320015-lbr2q4qh 104 7 in in IN cord-320015-lbr2q4qh 104 8 vaccinia vaccinia NN cord-320015-lbr2q4qh 104 9 virus virus NN cord-320015-lbr2q4qh 104 10 , , , cord-320015-lbr2q4qh 104 11 βHSD βHSD NNP cord-320015-lbr2q4qh 104 12 is be VBZ cord-320015-lbr2q4qh 104 13 thought think VBN cord-320015-lbr2q4qh 104 14 to to TO cord-320015-lbr2q4qh 104 15 trigger trigger VB cord-320015-lbr2q4qh 104 16 glucocorticoid glucocorticoid NN cord-320015-lbr2q4qh 104 17 synthesis synthesis NN cord-320015-lbr2q4qh 104 18 in in IN cord-320015-lbr2q4qh 104 19 infected infected JJ cord-320015-lbr2q4qh 104 20 animals animal NNS cord-320015-lbr2q4qh 104 21 and and CC cord-320015-lbr2q4qh 104 22 enhance enhance VB cord-320015-lbr2q4qh 104 23 virus virus NN cord-320015-lbr2q4qh 104 24 replication replication NN cord-320015-lbr2q4qh 104 25 by by IN cord-320015-lbr2q4qh 104 26 suppression suppression NN cord-320015-lbr2q4qh 104 27 of of IN cord-320015-lbr2q4qh 104 28 the the DT cord-320015-lbr2q4qh 104 29 overall overall JJ cord-320015-lbr2q4qh 104 30 immune immune JJ cord-320015-lbr2q4qh 104 31 response response NN cord-320015-lbr2q4qh 104 32 [ [ -LRB- cord-320015-lbr2q4qh 104 33 65 65 CD cord-320015-lbr2q4qh 104 34 , , , cord-320015-lbr2q4qh 104 35 66 66 CD cord-320015-lbr2q4qh 104 36 ] ] -RRB- cord-320015-lbr2q4qh 104 37 . . . cord-320015-lbr2q4qh 105 1 Likewise likewise RB cord-320015-lbr2q4qh 105 2 , , , cord-320015-lbr2q4qh 105 3 vCARD vcard RB cord-320015-lbr2q4qh 105 4 may may MD cord-320015-lbr2q4qh 105 5 inhibit inhibit VB cord-320015-lbr2q4qh 105 6 the the DT cord-320015-lbr2q4qh 105 7 induction induction NN cord-320015-lbr2q4qh 105 8 of of IN cord-320015-lbr2q4qh 105 9 interferon interferon NN cord-320015-lbr2q4qh 105 10 and/or and/or CC cord-320015-lbr2q4qh 105 11 apoptosis apoptosis NN cord-320015-lbr2q4qh 105 12 . . . cord-320015-lbr2q4qh 106 1 Induction induction NN cord-320015-lbr2q4qh 106 2 of of IN cord-320015-lbr2q4qh 106 3 IFN IFN NNP cord-320015-lbr2q4qh 106 4 and/or and/or CC cord-320015-lbr2q4qh 106 5 apoptosis apoptosis NN cord-320015-lbr2q4qh 106 6 follows follow VBZ cord-320015-lbr2q4qh 106 7 interaction interaction NN cord-320015-lbr2q4qh 106 8 of of IN cord-320015-lbr2q4qh 106 9 RIG rig NN cord-320015-lbr2q4qh 106 10 - - : cord-320015-lbr2q4qh 106 11 I I NNP cord-320015-lbr2q4qh 106 12 or or CC cord-320015-lbr2q4qh 106 13 MDA5 MDA5 NNP cord-320015-lbr2q4qh 106 14 , , , cord-320015-lbr2q4qh 106 15 cellular cellular JJ cord-320015-lbr2q4qh 106 16 proteins protein NNS cord-320015-lbr2q4qh 106 17 that that WDT cord-320015-lbr2q4qh 106 18 bind bind VBP cord-320015-lbr2q4qh 106 19 dsRNA dsrna NN cord-320015-lbr2q4qh 106 20 , , , cord-320015-lbr2q4qh 106 21 with with IN cord-320015-lbr2q4qh 106 22 downstream downstream JJ cord-320015-lbr2q4qh 106 23 effectors effector NNS cord-320015-lbr2q4qh 106 24 such such JJ cord-320015-lbr2q4qh 106 25 as as IN cord-320015-lbr2q4qh 106 26 MAVS MAVS NNP cord-320015-lbr2q4qh 106 27 and and CC cord-320015-lbr2q4qh 106 28 ISP-1 ISP-1 NNP cord-320015-lbr2q4qh 106 29 [ [ -LRB- cord-320015-lbr2q4qh 106 30 67 67 CD cord-320015-lbr2q4qh 106 31 ] ] -RRB- cord-320015-lbr2q4qh 106 32 [ [ -LRB- cord-320015-lbr2q4qh 106 33 68 68 CD cord-320015-lbr2q4qh 106 34 ] ] -RRB- cord-320015-lbr2q4qh 106 35 [ [ -LRB- cord-320015-lbr2q4qh 106 36 69 69 CD cord-320015-lbr2q4qh 106 37 ] ] -RRB- cord-320015-lbr2q4qh 106 38 [ [ -LRB- cord-320015-lbr2q4qh 106 39 70 70 CD cord-320015-lbr2q4qh 106 40 ] ] -RRB- cord-320015-lbr2q4qh 106 41 [ [ -LRB- cord-320015-lbr2q4qh 106 42 71 71 CD cord-320015-lbr2q4qh 106 43 ] ] -RRB- cord-320015-lbr2q4qh 106 44 [ [ -LRB- cord-320015-lbr2q4qh 106 45 72 72 CD cord-320015-lbr2q4qh 106 46 ] ] -RRB- cord-320015-lbr2q4qh 106 47 [ [ -LRB- cord-320015-lbr2q4qh 106 48 73 73 CD cord-320015-lbr2q4qh 106 49 ] ] -RRB- cord-320015-lbr2q4qh 106 50 . . . cord-320015-lbr2q4qh 107 1 Since since IN cord-320015-lbr2q4qh 107 2 RIG rig NN cord-320015-lbr2q4qh 107 3 - - : cord-320015-lbr2q4qh 107 4 I I NNP cord-320015-lbr2q4qh 107 5 / / SYM cord-320015-lbr2q4qh 107 6 MDA5 MDA5 NNP cord-320015-lbr2q4qh 107 7 and and CC cord-320015-lbr2q4qh 107 8 MAVS MAVS NNP cord-320015-lbr2q4qh 107 9 / / SYM cord-320015-lbr2q4qh 107 10 ISP-1 isp-1 JJ cord-320015-lbr2q4qh 107 11 interactions interaction NNS cord-320015-lbr2q4qh 107 12 are be VBP cord-320015-lbr2q4qh 107 13 mediated mediate VBN cord-320015-lbr2q4qh 107 14 by by IN cord-320015-lbr2q4qh 107 15 CARD card NN cord-320015-lbr2q4qh 107 16 motifs motif NNS cord-320015-lbr2q4qh 107 17 , , , cord-320015-lbr2q4qh 107 18 it -PRON- PRP cord-320015-lbr2q4qh 107 19 is be VBZ cord-320015-lbr2q4qh 107 20 possible possible JJ cord-320015-lbr2q4qh 107 21 that that IN cord-320015-lbr2q4qh 107 22 a a DT cord-320015-lbr2q4qh 107 23 viral viral JJ cord-320015-lbr2q4qh 107 24 CARD card NN cord-320015-lbr2q4qh 107 25 - - HYPH cord-320015-lbr2q4qh 107 26 containing contain VBG cord-320015-lbr2q4qh 107 27 protein protein NN cord-320015-lbr2q4qh 107 28 might may MD cord-320015-lbr2q4qh 107 29 bind bind VB cord-320015-lbr2q4qh 107 30 either either CC cord-320015-lbr2q4qh 107 31 one one CD cord-320015-lbr2q4qh 107 32 or or CC cord-320015-lbr2q4qh 107 33 both both DT cord-320015-lbr2q4qh 107 34 of of IN cord-320015-lbr2q4qh 107 35 these these DT cord-320015-lbr2q4qh 107 36 proteins protein NNS cord-320015-lbr2q4qh 107 37 and and CC cord-320015-lbr2q4qh 107 38 short short JJ cord-320015-lbr2q4qh 107 39 - - HYPH cord-320015-lbr2q4qh 107 40 circuit circuit NN cord-320015-lbr2q4qh 107 41 the the DT cord-320015-lbr2q4qh 107 42 IFN IFN NNP cord-320015-lbr2q4qh 107 43 induction induction NN cord-320015-lbr2q4qh 107 44 pathway pathway NN cord-320015-lbr2q4qh 107 45 [ [ -LRB- cord-320015-lbr2q4qh 107 46 74 74 CD cord-320015-lbr2q4qh 107 47 ] ] -RRB- cord-320015-lbr2q4qh 107 48 . . . cord-320015-lbr2q4qh 108 1 The the DT cord-320015-lbr2q4qh 108 2 RNAse RNAse NNP cord-320015-lbr2q4qh 108 3 III III NNP cord-320015-lbr2q4qh 108 4 - - HYPH cord-320015-lbr2q4qh 108 5 like like JJ cord-320015-lbr2q4qh 108 6 protein protein NN cord-320015-lbr2q4qh 108 7 is be VBZ cord-320015-lbr2q4qh 108 8 found find VBN cord-320015-lbr2q4qh 108 9 within within IN cord-320015-lbr2q4qh 108 10 all all DT cord-320015-lbr2q4qh 108 11 iridoviruses iridoviruse NNS cord-320015-lbr2q4qh 108 12 and and CC cord-320015-lbr2q4qh 108 13 has have VBZ cord-320015-lbr2q4qh 108 14 been be VBN cord-320015-lbr2q4qh 108 15 postulated postulate VBN cord-320015-lbr2q4qh 108 16 to to TO cord-320015-lbr2q4qh 108 17 block block VB cord-320015-lbr2q4qh 108 18 siRNA siRNA NNP cord-320015-lbr2q4qh 108 19 - - HYPH cord-320015-lbr2q4qh 108 20 mediated mediate VBN cord-320015-lbr2q4qh 108 21 interference interference NN cord-320015-lbr2q4qh 108 22 , , , cord-320015-lbr2q4qh 108 23 or or CC cord-320015-lbr2q4qh 108 24 to to TO cord-320015-lbr2q4qh 108 25 play play VB cord-320015-lbr2q4qh 108 26 a a DT cord-320015-lbr2q4qh 108 27 role role NN cord-320015-lbr2q4qh 108 28 in in IN cord-320015-lbr2q4qh 108 29 the the DT cord-320015-lbr2q4qh 108 30 processing processing NN cord-320015-lbr2q4qh 108 31 of of IN cord-320015-lbr2q4qh 108 32 viral viral JJ cord-320015-lbr2q4qh 108 33 mRNAs mrnas CD cord-320015-lbr2q4qh 108 34 [ [ -LRB- cord-320015-lbr2q4qh 108 35 75 75 CD cord-320015-lbr2q4qh 108 36 ] ] -RRB- cord-320015-lbr2q4qh 108 37 . . . cord-320015-lbr2q4qh 109 1 Alternatively alternatively RB cord-320015-lbr2q4qh 109 2 , , , cord-320015-lbr2q4qh 109 3 viral viral JJ cord-320015-lbr2q4qh 109 4 RNAse RNAse NNP cord-320015-lbr2q4qh 109 5 III III NNP cord-320015-lbr2q4qh 109 6 could could MD cord-320015-lbr2q4qh 109 7 act act VB cord-320015-lbr2q4qh 109 8 like like IN cord-320015-lbr2q4qh 109 9 vaccinia vaccinia NN cord-320015-lbr2q4qh 109 10 virus virus NN cord-320015-lbr2q4qh 109 11 E3L e3l NN cord-320015-lbr2q4qh 109 12 and and CC cord-320015-lbr2q4qh 109 13 bind bind VB cord-320015-lbr2q4qh 109 14 and/or and/or CC cord-320015-lbr2q4qh 109 15 degrade degrade VB cord-320015-lbr2q4qh 109 16 dsRNA dsrna NN cord-320015-lbr2q4qh 109 17 and and CC cord-320015-lbr2q4qh 109 18 block block VB cord-320015-lbr2q4qh 109 19 the the DT cord-320015-lbr2q4qh 109 20 activation activation NN cord-320015-lbr2q4qh 109 21 of of IN cord-320015-lbr2q4qh 109 22 PKR PKR NNP cord-320015-lbr2q4qh 109 23 , , , cord-320015-lbr2q4qh 109 24 the the DT cord-320015-lbr2q4qh 109 25 induction induction NN cord-320015-lbr2q4qh 109 26 of of IN cord-320015-lbr2q4qh 109 27 interferon interferon NN cord-320015-lbr2q4qh 109 28 , , , cord-320015-lbr2q4qh 109 29 and and CC cord-320015-lbr2q4qh 109 30 the the DT cord-320015-lbr2q4qh 109 31 inhibition inhibition NN cord-320015-lbr2q4qh 109 32 of of IN cord-320015-lbr2q4qh 109 33 protein protein NN cord-320015-lbr2q4qh 109 34 synthesis synthesis NN cord-320015-lbr2q4qh 109 35 [ [ -LRB- cord-320015-lbr2q4qh 109 36 62 62 CD cord-320015-lbr2q4qh 109 37 ] ] -RRB- cord-320015-lbr2q4qh 109 38 . . . cord-320015-lbr2q4qh 110 1 As as IN cord-320015-lbr2q4qh 110 2 with with IN cord-320015-lbr2q4qh 110 3 vaccinia vaccinia NN cord-320015-lbr2q4qh 110 4 virus virus NN cord-320015-lbr2q4qh 110 5 [ [ -LRB- cord-320015-lbr2q4qh 110 6 76 76 CD cord-320015-lbr2q4qh 110 7 ] ] -RRB- cord-320015-lbr2q4qh 110 8 [ [ -LRB- cord-320015-lbr2q4qh 110 9 77 77 CD cord-320015-lbr2q4qh 110 10 ] ] -RRB- cord-320015-lbr2q4qh 110 11 [ [ -LRB- cord-320015-lbr2q4qh 110 12 78 78 CD cord-320015-lbr2q4qh 110 13 ] ] -RRB- cord-320015-lbr2q4qh 110 14 , , , cord-320015-lbr2q4qh 110 15 iridoviruses iridoviruse NNS cord-320015-lbr2q4qh 110 16 that that WDT cord-320015-lbr2q4qh 110 17 infect infect VBP cord-320015-lbr2q4qh 110 18 ectothermic ectothermic JJ cord-320015-lbr2q4qh 110 19 animals animal NNS cord-320015-lbr2q4qh 110 20 encode encode VBP cord-320015-lbr2q4qh 110 21 a a DT cord-320015-lbr2q4qh 110 22 series series NN cord-320015-lbr2q4qh 110 23 of of IN cord-320015-lbr2q4qh 110 24 proteins protein NNS cord-320015-lbr2q4qh 110 25 whose whose WP$ cord-320015-lbr2q4qh 110 26 function function NN cord-320015-lbr2q4qh 110 27 is be VBZ cord-320015-lbr2q4qh 110 28 to to TO cord-320015-lbr2q4qh 110 29 ensure ensure VB cord-320015-lbr2q4qh 110 30 an an DT cord-320015-lbr2q4qh 110 31 adequate adequate JJ cord-320015-lbr2q4qh 110 32 supply supply NN cord-320015-lbr2q4qh 110 33 of of IN cord-320015-lbr2q4qh 110 34 nucleotides nucleotide NNS cord-320015-lbr2q4qh 110 35 for for IN cord-320015-lbr2q4qh 110 36 viral viral JJ cord-320015-lbr2q4qh 110 37 DNA dna NN cord-320015-lbr2q4qh 110 38 synthesis synthesis NN cord-320015-lbr2q4qh 110 39 . . . cord-320015-lbr2q4qh 111 1 These these DT cord-320015-lbr2q4qh 111 2 enzymes enzyme NNS cord-320015-lbr2q4qh 111 3 include include VBP cord-320015-lbr2q4qh 111 4 the the DT cord-320015-lbr2q4qh 111 5 two two CD cord-320015-lbr2q4qh 111 6 subunits subunit NNS cord-320015-lbr2q4qh 111 7 of of IN cord-320015-lbr2q4qh 111 8 ribonucleotide ribonucleotide NN cord-320015-lbr2q4qh 111 9 reductase reductase NN cord-320015-lbr2q4qh 111 10 [ [ -LRB- cord-320015-lbr2q4qh 111 11 79 79 CD cord-320015-lbr2q4qh 111 12 , , , cord-320015-lbr2q4qh 111 13 80 80 CD cord-320015-lbr2q4qh 111 14 ] ] -RRB- cord-320015-lbr2q4qh 111 15 , , , cord-320015-lbr2q4qh 111 16 thymidine thymidine NN cord-320015-lbr2q4qh 111 17 kinase kinase NN cord-320015-lbr2q4qh 111 18 [ [ -LRB- cord-320015-lbr2q4qh 111 19 30 30 CD cord-320015-lbr2q4qh 111 20 ] ] -RRB- cord-320015-lbr2q4qh 111 21 , , , cord-320015-lbr2q4qh 111 22 thymidylate thymidylate NNP cord-320015-lbr2q4qh 111 23 synthase synthase NN cord-320015-lbr2q4qh 111 24 , , , cord-320015-lbr2q4qh 111 25 dUTPase dUTPase '' cord-320015-lbr2q4qh 111 26 [ [ -LRB- cord-320015-lbr2q4qh 111 27 81 81 CD cord-320015-lbr2q4qh 111 28 ] ] -RRB- cord-320015-lbr2q4qh 111 29 , , , cord-320015-lbr2q4qh 111 30 purine purine NN cord-320015-lbr2q4qh 111 31 nucleoside nucleoside NN cord-320015-lbr2q4qh 111 32 phosphorylase phosphorylase NN cord-320015-lbr2q4qh 111 33 ( ( -LRB- cord-320015-lbr2q4qh 111 34 PNP PNP NNP cord-320015-lbr2q4qh 111 35 ) ) -RRB- cord-320015-lbr2q4qh 111 36 , , , cord-320015-lbr2q4qh 111 37 and and CC cord-320015-lbr2q4qh 111 38 a a DT cord-320015-lbr2q4qh 111 39 dihydrofolate dihydrofolate NN cord-320015-lbr2q4qh 111 40 reductase reductase NN cord-320015-lbr2q4qh 111 41 homolog homolog NN cord-320015-lbr2q4qh 111 42 [ [ -LRB- cord-320015-lbr2q4qh 111 43 12 12 CD cord-320015-lbr2q4qh 111 44 , , , cord-320015-lbr2q4qh 111 45 82 82 CD cord-320015-lbr2q4qh 111 46 ] ] -RRB- cord-320015-lbr2q4qh 111 47 . . . cord-320015-lbr2q4qh 112 1 Not not RB cord-320015-lbr2q4qh 112 2 all all DT cord-320015-lbr2q4qh 112 3 of of IN cord-320015-lbr2q4qh 112 4 these these DT cord-320015-lbr2q4qh 112 5 putative putative JJ cord-320015-lbr2q4qh 112 6 enzymes enzyme NNS cord-320015-lbr2q4qh 112 7 are be VBP cord-320015-lbr2q4qh 112 8 found find VBN cord-320015-lbr2q4qh 112 9 within within IN cord-320015-lbr2q4qh 112 10 each each DT cord-320015-lbr2q4qh 112 11 viral viral JJ cord-320015-lbr2q4qh 112 12 species specie NNS cord-320015-lbr2q4qh 112 13 , , , cord-320015-lbr2q4qh 112 14 and and CC cord-320015-lbr2q4qh 112 15 it -PRON- PRP cord-320015-lbr2q4qh 112 16 is be VBZ cord-320015-lbr2q4qh 112 17 postulated postulate VBN cord-320015-lbr2q4qh 112 18 that that IN cord-320015-lbr2q4qh 112 19 specific specific JJ cord-320015-lbr2q4qh 112 20 enzymes enzyme NNS cord-320015-lbr2q4qh 112 21 might may MD cord-320015-lbr2q4qh 112 22 be be VB cord-320015-lbr2q4qh 112 23 needed need VBN cord-320015-lbr2q4qh 112 24 in in IN cord-320015-lbr2q4qh 112 25 certain certain JJ cord-320015-lbr2q4qh 112 26 hosts host NNS cord-320015-lbr2q4qh 112 27 . . . cord-320015-lbr2q4qh 113 1 For for IN cord-320015-lbr2q4qh 113 2 example example NN cord-320015-lbr2q4qh 113 3 , , , cord-320015-lbr2q4qh 113 4 PNP PNP NNP cord-320015-lbr2q4qh 113 5 is be VBZ cord-320015-lbr2q4qh 113 6 found find VBN cord-320015-lbr2q4qh 113 7 only only RB cord-320015-lbr2q4qh 113 8 within within IN cord-320015-lbr2q4qh 113 9 GIV GIV NNP cord-320015-lbr2q4qh 113 10 and and CC cord-320015-lbr2q4qh 113 11 it -PRON- PRP cord-320015-lbr2q4qh 113 12 has have VBZ cord-320015-lbr2q4qh 113 13 been be VBN cord-320015-lbr2q4qh 113 14 suggested suggest VBN cord-320015-lbr2q4qh 113 15 that that IN cord-320015-lbr2q4qh 113 16 because because IN cord-320015-lbr2q4qh 113 17 GIV GIV NNP cord-320015-lbr2q4qh 113 18 's 's POS cord-320015-lbr2q4qh 113 19 natural natural JJ cord-320015-lbr2q4qh 113 20 host host NN cord-320015-lbr2q4qh 113 21 , , , cord-320015-lbr2q4qh 113 22 the the DT cord-320015-lbr2q4qh 113 23 grouper grouper NN cord-320015-lbr2q4qh 113 24 , , , cord-320015-lbr2q4qh 113 25 might may MD cord-320015-lbr2q4qh 113 26 not not RB cord-320015-lbr2q4qh 113 27 be be VB cord-320015-lbr2q4qh 113 28 able able JJ cord-320015-lbr2q4qh 113 29 to to TO cord-320015-lbr2q4qh 113 30 supply supply VB cord-320015-lbr2q4qh 113 31 the the DT cord-320015-lbr2q4qh 113 32 purine purine NN cord-320015-lbr2q4qh 113 33 nucleotides nucleotide NNS cord-320015-lbr2q4qh 113 34 required require VBN cord-320015-lbr2q4qh 113 35 for for IN cord-320015-lbr2q4qh 113 36 viral viral JJ cord-320015-lbr2q4qh 113 37 replication replication NN cord-320015-lbr2q4qh 113 38 the the DT cord-320015-lbr2q4qh 113 39 virus virus NN cord-320015-lbr2q4qh 113 40 encodes encode VBZ cord-320015-lbr2q4qh 113 41 its -PRON- PRP$ cord-320015-lbr2q4qh 113 42 own own JJ cord-320015-lbr2q4qh 113 43 PNP PNP NNP cord-320015-lbr2q4qh 113 44 [ [ -LRB- cord-320015-lbr2q4qh 113 45 82 82 CD cord-320015-lbr2q4qh 113 46 ] ] -RRB- cord-320015-lbr2q4qh 113 47 . . . cord-320015-lbr2q4qh 114 1 Currently currently RB cord-320015-lbr2q4qh 114 2 there there EX cord-320015-lbr2q4qh 114 3 are be VBP cord-320015-lbr2q4qh 114 4 seven seven CD cord-320015-lbr2q4qh 114 5 completely completely RB cord-320015-lbr2q4qh 114 6 - - HYPH cord-320015-lbr2q4qh 114 7 sequenced sequence VBN cord-320015-lbr2q4qh 114 8 ranavirus ranavirus NN cord-320015-lbr2q4qh 114 9 genomes genome NNS cord-320015-lbr2q4qh 114 10 ranging range VBG cord-320015-lbr2q4qh 114 11 in in IN cord-320015-lbr2q4qh 114 12 size size NN cord-320015-lbr2q4qh 114 13 from from IN cord-320015-lbr2q4qh 114 14 105 105 CD cord-320015-lbr2q4qh 114 15 - - SYM cord-320015-lbr2q4qh 114 16 140 140 CD cord-320015-lbr2q4qh 114 17 kbp kbp NNS cord-320015-lbr2q4qh 114 18 ( ( -LRB- cord-320015-lbr2q4qh 114 19 Table table NN cord-320015-lbr2q4qh 114 20 1 1 CD cord-320015-lbr2q4qh 114 21 ) ) -RRB- cord-320015-lbr2q4qh 114 22 . . . cord-320015-lbr2q4qh 115 1 Whole whole JJ cord-320015-lbr2q4qh 115 2 genome genome NN cord-320015-lbr2q4qh 115 3 dot dot NN cord-320015-lbr2q4qh 115 4 plot plot NN cord-320015-lbr2q4qh 115 5 analyses analysis NNS cord-320015-lbr2q4qh 115 6 show show VBP cord-320015-lbr2q4qh 115 7 that that IN cord-320015-lbr2q4qh 115 8 there there EX cord-320015-lbr2q4qh 115 9 are be VBP cord-320015-lbr2q4qh 115 10 three three CD cord-320015-lbr2q4qh 115 11 distinct distinct JJ cord-320015-lbr2q4qh 115 12 genomic genomic JJ cord-320015-lbr2q4qh 115 13 organizational organizational JJ cord-320015-lbr2q4qh 115 14 profiles profile NNS cord-320015-lbr2q4qh 115 15 among among IN cord-320015-lbr2q4qh 115 16 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 115 17 that that WDT cord-320015-lbr2q4qh 115 18 are be VBP cord-320015-lbr2q4qh 115 19 based base VBN cord-320015-lbr2q4qh 115 20 on on IN cord-320015-lbr2q4qh 115 21 the the DT cord-320015-lbr2q4qh 115 22 conservation conservation NN cord-320015-lbr2q4qh 115 23 of of IN cord-320015-lbr2q4qh 115 24 gene gene NN cord-320015-lbr2q4qh 115 25 order order NN cord-320015-lbr2q4qh 115 26 [ [ -LRB- cord-320015-lbr2q4qh 115 27 12 12 CD cord-320015-lbr2q4qh 115 28 ] ] -RRB- cord-320015-lbr2q4qh 115 29 : : : cord-320015-lbr2q4qh 115 30 FV3-like fv3-like IN cord-320015-lbr2q4qh 115 31 viruses virus NNS cord-320015-lbr2q4qh 115 32 ( ( -LRB- cord-320015-lbr2q4qh 115 33 i.e. i.e. FW cord-320015-lbr2q4qh 115 34 FV3 FV3 NNP cord-320015-lbr2q4qh 115 35 , , , cord-320015-lbr2q4qh 115 36 TFV TFV NNP cord-320015-lbr2q4qh 115 37 and and CC cord-320015-lbr2q4qh 115 38 STIV STIV NNP cord-320015-lbr2q4qh 115 39 ) ) -RRB- cord-320015-lbr2q4qh 115 40 , , , cord-320015-lbr2q4qh 115 41 ATV ATV NNP cord-320015-lbr2q4qh 115 42 - - HYPH cord-320015-lbr2q4qh 115 43 like like JJ cord-320015-lbr2q4qh 115 44 viruses virus NNS cord-320015-lbr2q4qh 115 45 ( ( -LRB- cord-320015-lbr2q4qh 115 46 i.e. i.e. FW cord-320015-lbr2q4qh 115 47 ATV ATV NNP cord-320015-lbr2q4qh 115 48 and and CC cord-320015-lbr2q4qh 115 49 EHNV EHNV NNP cord-320015-lbr2q4qh 115 50 ) ) -RRB- cord-320015-lbr2q4qh 115 51 and and CC cord-320015-lbr2q4qh 115 52 GIV giv NN cord-320015-lbr2q4qh 115 53 - - HYPH cord-320015-lbr2q4qh 115 54 like like JJ cord-320015-lbr2q4qh 115 55 viruses virus NNS cord-320015-lbr2q4qh 115 56 ( ( -LRB- cord-320015-lbr2q4qh 115 57 i.e. i.e. FW cord-320015-lbr2q4qh 115 58 SGIV SGIV NNP cord-320015-lbr2q4qh 115 59 and and CC cord-320015-lbr2q4qh 115 60 GIV GIV NNP cord-320015-lbr2q4qh 115 61 ) ) -RRB- cord-320015-lbr2q4qh 115 62 . . . cord-320015-lbr2q4qh 116 1 Dot dot NN cord-320015-lbr2q4qh 116 2 plot plot NN cord-320015-lbr2q4qh 116 3 comparisons comparison NNS cord-320015-lbr2q4qh 116 4 of of IN cord-320015-lbr2q4qh 116 5 viruses virus NNS cord-320015-lbr2q4qh 116 6 within within IN cord-320015-lbr2q4qh 116 7 each each DT cord-320015-lbr2q4qh 116 8 group group NN cord-320015-lbr2q4qh 116 9 show show VBP cord-320015-lbr2q4qh 116 10 complete complete JJ cord-320015-lbr2q4qh 116 11 co co NN cord-320015-lbr2q4qh 116 12 - - NN cord-320015-lbr2q4qh 116 13 linearity linearity NN cord-320015-lbr2q4qh 116 14 . . . cord-320015-lbr2q4qh 117 1 However however RB cord-320015-lbr2q4qh 117 2 , , , cord-320015-lbr2q4qh 117 3 comparison comparison NN cord-320015-lbr2q4qh 117 4 of of IN cord-320015-lbr2q4qh 117 5 ATV ATV NNP cord-320015-lbr2q4qh 117 6 - - HYPH cord-320015-lbr2q4qh 117 7 like like JJ cord-320015-lbr2q4qh 117 8 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 117 9 with with IN cord-320015-lbr2q4qh 117 10 FV3-like fv3-like NN cord-320015-lbr2q4qh 117 11 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 117 12 detected detect VBD cord-320015-lbr2q4qh 117 13 two two CD cord-320015-lbr2q4qh 117 14 large large JJ cord-320015-lbr2q4qh 117 15 inversions inversion NNS cord-320015-lbr2q4qh 117 16 , , , cord-320015-lbr2q4qh 117 17 whereas whereas IN cord-320015-lbr2q4qh 117 18 comparing compare VBG cord-320015-lbr2q4qh 117 19 either either DT cord-320015-lbr2q4qh 117 20 of of IN cord-320015-lbr2q4qh 117 21 these these DT cord-320015-lbr2q4qh 117 22 groups group NNS cord-320015-lbr2q4qh 117 23 to to IN cord-320015-lbr2q4qh 117 24 GIV giv VB cord-320015-lbr2q4qh 117 25 - - HYPH cord-320015-lbr2q4qh 117 26 like like JJ cord-320015-lbr2q4qh 117 27 viruses virus NNS cord-320015-lbr2q4qh 117 28 revealed reveal VBD cord-320015-lbr2q4qh 117 29 very very RB cord-320015-lbr2q4qh 117 30 little little JJ cord-320015-lbr2q4qh 117 31 conservation conservation NN cord-320015-lbr2q4qh 117 32 of of IN cord-320015-lbr2q4qh 117 33 gene gene NN cord-320015-lbr2q4qh 117 34 order order NN cord-320015-lbr2q4qh 117 35 [ [ -LRB- cord-320015-lbr2q4qh 117 36 11 11 CD cord-320015-lbr2q4qh 117 37 , , , cord-320015-lbr2q4qh 117 38 83 83 CD cord-320015-lbr2q4qh 117 39 ] ] -RRB- cord-320015-lbr2q4qh 117 40 . . . cord-320015-lbr2q4qh 118 1 In in IN cord-320015-lbr2q4qh 118 2 the the DT cord-320015-lbr2q4qh 118 3 latter latter JJ cord-320015-lbr2q4qh 118 4 case case NN cord-320015-lbr2q4qh 118 5 , , , cord-320015-lbr2q4qh 118 6 gene gene NN cord-320015-lbr2q4qh 118 7 order order NN cord-320015-lbr2q4qh 118 8 is be VBZ cord-320015-lbr2q4qh 118 9 so so RB cord-320015-lbr2q4qh 118 10 jumbled jumbled JJ cord-320015-lbr2q4qh 118 11 between between IN cord-320015-lbr2q4qh 118 12 FV3 FV3 NNP cord-320015-lbr2q4qh 118 13 / / SYM cord-320015-lbr2q4qh 118 14 ATV ATV NNP cord-320015-lbr2q4qh 118 15 - - HYPH cord-320015-lbr2q4qh 118 16 like like JJ cord-320015-lbr2q4qh 118 17 viruses virus NNS cord-320015-lbr2q4qh 118 18 and and CC cord-320015-lbr2q4qh 118 19 SGIV SGIV NNP cord-320015-lbr2q4qh 118 20 / / SYM cord-320015-lbr2q4qh 118 21 GIV giv NN cord-320015-lbr2q4qh 118 22 - - HYPH cord-320015-lbr2q4qh 118 23 like like JJ cord-320015-lbr2q4qh 118 24 viruses virus NNS cord-320015-lbr2q4qh 118 25 that that WDT cord-320015-lbr2q4qh 118 26 any any DT cord-320015-lbr2q4qh 118 27 trace trace NN cord-320015-lbr2q4qh 118 28 of of IN cord-320015-lbr2q4qh 118 29 the the DT cord-320015-lbr2q4qh 118 30 45 45 CD cord-320015-lbr2q4qh 118 31 degree degree NN cord-320015-lbr2q4qh 118 32 diagonal diagonal JJ cord-320015-lbr2q4qh 118 33 line line NN cord-320015-lbr2q4qh 118 34 , , , cord-320015-lbr2q4qh 118 35 indicative indicative JJ cord-320015-lbr2q4qh 118 36 of of IN cord-320015-lbr2q4qh 118 37 complete complete JJ cord-320015-lbr2q4qh 118 38 sequence sequence NN cord-320015-lbr2q4qh 118 39 alignment alignment NN cord-320015-lbr2q4qh 118 40 between between IN cord-320015-lbr2q4qh 118 41 genomes genome NNS cord-320015-lbr2q4qh 118 42 in in IN cord-320015-lbr2q4qh 118 43 dot dot NN cord-320015-lbr2q4qh 118 44 plots plot NNS cord-320015-lbr2q4qh 118 45 , , , cord-320015-lbr2q4qh 118 46 is be VBZ cord-320015-lbr2q4qh 118 47 lost lose VBN cord-320015-lbr2q4qh 118 48 . . . cord-320015-lbr2q4qh 119 1 Interestingly interestingly RB cord-320015-lbr2q4qh 119 2 , , , cord-320015-lbr2q4qh 119 3 ranavirus ranavirus NNP cord-320015-lbr2q4qh 119 4 genomic genomic NNP cord-320015-lbr2q4qh 119 5 organization organization NN cord-320015-lbr2q4qh 119 6 is be VBZ cord-320015-lbr2q4qh 119 7 markedly markedly RB cord-320015-lbr2q4qh 119 8 different different JJ cord-320015-lbr2q4qh 119 9 from from IN cord-320015-lbr2q4qh 119 10 that that DT cord-320015-lbr2q4qh 119 11 of of IN cord-320015-lbr2q4qh 119 12 poxviruses poxvirus NNS cord-320015-lbr2q4qh 119 13 . . . cord-320015-lbr2q4qh 120 1 For for IN cord-320015-lbr2q4qh 120 2 example example NN cord-320015-lbr2q4qh 120 3 , , , cord-320015-lbr2q4qh 120 4 all all DT cord-320015-lbr2q4qh 120 5 members member NNS cord-320015-lbr2q4qh 120 6 of of IN cord-320015-lbr2q4qh 120 7 the the DT cord-320015-lbr2q4qh 120 8 subfamily subfamily NN cord-320015-lbr2q4qh 120 9 Chordopoxvirinae Chordopoxvirinae NNP cord-320015-lbr2q4qh 120 10 share share VBP cord-320015-lbr2q4qh 120 11 a a DT cord-320015-lbr2q4qh 120 12 conserved conserved JJ cord-320015-lbr2q4qh 120 13 genomic genomic JJ cord-320015-lbr2q4qh 120 14 core core NN cord-320015-lbr2q4qh 120 15 structure structure NN cord-320015-lbr2q4qh 120 16 wherein wherein WRB cord-320015-lbr2q4qh 120 17 gene gene NN cord-320015-lbr2q4qh 120 18 order order NN cord-320015-lbr2q4qh 120 19 , , , cord-320015-lbr2q4qh 120 20 with with IN cord-320015-lbr2q4qh 120 21 the the DT cord-320015-lbr2q4qh 120 22 single single JJ cord-320015-lbr2q4qh 120 23 exception exception NN cord-320015-lbr2q4qh 120 24 of of IN cord-320015-lbr2q4qh 120 25 avipoxviruses avipoxviruse NNS cord-320015-lbr2q4qh 120 26 , , , cord-320015-lbr2q4qh 120 27 is be VBZ cord-320015-lbr2q4qh 120 28 conserved conserve VBN cord-320015-lbr2q4qh 120 29 [ [ -LRB- cord-320015-lbr2q4qh 120 30 84 84 CD cord-320015-lbr2q4qh 120 31 ] ] -RRB- cord-320015-lbr2q4qh 120 32 . . . cord-320015-lbr2q4qh 121 1 However however RB cord-320015-lbr2q4qh 121 2 , , , cord-320015-lbr2q4qh 121 3 despite despite IN cord-320015-lbr2q4qh 121 4 the the DT cord-320015-lbr2q4qh 121 5 conservation conservation NN cord-320015-lbr2q4qh 121 6 of of IN cord-320015-lbr2q4qh 121 7 gene gene NN cord-320015-lbr2q4qh 121 8 order order NN cord-320015-lbr2q4qh 121 9 , , , cord-320015-lbr2q4qh 121 10 global global JJ cord-320015-lbr2q4qh 121 11 sequence sequence NN cord-320015-lbr2q4qh 121 12 alignments alignment NNS cord-320015-lbr2q4qh 121 13 show show VBP cord-320015-lbr2q4qh 121 14 that that DT cord-320015-lbr2q4qh 121 15 sequence sequence NN cord-320015-lbr2q4qh 121 16 identity identity NN cord-320015-lbr2q4qh 121 17 among among IN cord-320015-lbr2q4qh 121 18 members member NNS cord-320015-lbr2q4qh 121 19 of of IN cord-320015-lbr2q4qh 121 20 the the DT cord-320015-lbr2q4qh 121 21 Chordopoxvirinae Chordopoxvirinae NNP cord-320015-lbr2q4qh 121 22 is be VBZ cord-320015-lbr2q4qh 121 23 only only RB cord-320015-lbr2q4qh 121 24 27 27 CD cord-320015-lbr2q4qh 121 25 - - SYM cord-320015-lbr2q4qh 121 26 29 29 CD cord-320015-lbr2q4qh 121 27 % % NN cord-320015-lbr2q4qh 121 28 [ [ -LRB- cord-320015-lbr2q4qh 121 29 85 85 CD cord-320015-lbr2q4qh 121 30 , , , cord-320015-lbr2q4qh 121 31 86 86 CD cord-320015-lbr2q4qh 121 32 ] ] -RRB- cord-320015-lbr2q4qh 121 33 . . . cord-320015-lbr2q4qh 122 1 Ranaviruses Ranaviruses NNP cord-320015-lbr2q4qh 122 2 , , , cord-320015-lbr2q4qh 122 3 on on IN cord-320015-lbr2q4qh 122 4 the the DT cord-320015-lbr2q4qh 122 5 other other JJ cord-320015-lbr2q4qh 122 6 hand hand NN cord-320015-lbr2q4qh 122 7 , , , cord-320015-lbr2q4qh 122 8 share share VB cord-320015-lbr2q4qh 122 9 a a DT cord-320015-lbr2q4qh 122 10 much much RB cord-320015-lbr2q4qh 122 11 greater great JJR cord-320015-lbr2q4qh 122 12 sequence sequence NN cord-320015-lbr2q4qh 122 13 identity identity NN cord-320015-lbr2q4qh 122 14 ( ( -LRB- cord-320015-lbr2q4qh 122 15 ~72 ~72 NFP cord-320015-lbr2q4qh 122 16 -95 -95 NN cord-320015-lbr2q4qh 122 17 % % NN cord-320015-lbr2q4qh 122 18 within within IN cord-320015-lbr2q4qh 122 19 the the DT cord-320015-lbr2q4qh 122 20 MCP MCP NNP cord-320015-lbr2q4qh 122 21 ) ) -RRB- cord-320015-lbr2q4qh 122 22 yet yet CC cord-320015-lbr2q4qh 122 23 have have VBP cord-320015-lbr2q4qh 122 24 three three CD cord-320015-lbr2q4qh 122 25 widely widely RB cord-320015-lbr2q4qh 122 26 divergent divergent JJ cord-320015-lbr2q4qh 122 27 genomic genomic JJ cord-320015-lbr2q4qh 122 28 organizations organization NNS cord-320015-lbr2q4qh 122 29 . . . cord-320015-lbr2q4qh 123 1 Moreover moreover RB cord-320015-lbr2q4qh 123 2 , , , cord-320015-lbr2q4qh 123 3 as as IN cord-320015-lbr2q4qh 123 4 additional additional JJ cord-320015-lbr2q4qh 123 5 ranavirus ranavirus NN cord-320015-lbr2q4qh 123 6 genomes genome NNS cord-320015-lbr2q4qh 123 7 are be VBP cord-320015-lbr2q4qh 123 8 sequenced sequence VBN cord-320015-lbr2q4qh 123 9 , , , cord-320015-lbr2q4qh 123 10 we -PRON- PRP cord-320015-lbr2q4qh 123 11 may may MD cord-320015-lbr2q4qh 123 12 discover discover VB cord-320015-lbr2q4qh 123 13 novel novel JJ cord-320015-lbr2q4qh 123 14 genomic genomic JJ cord-320015-lbr2q4qh 123 15 morphotypes morphotype NNS cord-320015-lbr2q4qh 123 16 that that WDT cord-320015-lbr2q4qh 123 17 will will MD cord-320015-lbr2q4qh 123 18 elucidate elucidate VB cord-320015-lbr2q4qh 123 19 the the DT cord-320015-lbr2q4qh 123 20 evolutionary evolutionary JJ cord-320015-lbr2q4qh 123 21 relatedness relatedness NN cord-320015-lbr2q4qh 123 22 of of IN cord-320015-lbr2q4qh 123 23 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 123 24 . . . cord-320015-lbr2q4qh 124 1 Examination examination NN cord-320015-lbr2q4qh 124 2 of of IN cord-320015-lbr2q4qh 124 3 complete complete JJ cord-320015-lbr2q4qh 124 4 ranavirus ranavirus NNP cord-320015-lbr2q4qh 124 5 genomic genomic JJ cord-320015-lbr2q4qh 124 6 sequences sequence NNS cord-320015-lbr2q4qh 124 7 , , , cord-320015-lbr2q4qh 124 8 including include VBG cord-320015-lbr2q4qh 124 9 whole whole JJ cord-320015-lbr2q4qh 124 10 genome genome NN cord-320015-lbr2q4qh 124 11 dot dot NN cord-320015-lbr2q4qh 124 12 plot plot NN cord-320015-lbr2q4qh 124 13 and and CC cord-320015-lbr2q4qh 124 14 phylogenetic phylogenetic JJ cord-320015-lbr2q4qh 124 15 analyses analysis NNS cord-320015-lbr2q4qh 124 16 of of IN cord-320015-lbr2q4qh 124 17 ranavirus ranavirus NNP cord-320015-lbr2q4qh 124 18 core core NN cord-320015-lbr2q4qh 124 19 genes gene NNS cord-320015-lbr2q4qh 124 20 , , , cord-320015-lbr2q4qh 124 21 suggested suggest VBD cord-320015-lbr2q4qh 124 22 that that IN cord-320015-lbr2q4qh 124 23 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 124 24 have have VBP cord-320015-lbr2q4qh 124 25 undergone undergo VBN cord-320015-lbr2q4qh 124 26 a a DT cord-320015-lbr2q4qh 124 27 number number NN cord-320015-lbr2q4qh 124 28 of of IN cord-320015-lbr2q4qh 124 29 evolutionarily evolutionarily RB cord-320015-lbr2q4qh 124 30 - - HYPH cord-320015-lbr2q4qh 124 31 recent recent JJ cord-320015-lbr2q4qh 124 32 host host NN cord-320015-lbr2q4qh 124 33 shifts shift NNS cord-320015-lbr2q4qh 124 34 [ [ -LRB- cord-320015-lbr2q4qh 124 35 12 12 CD cord-320015-lbr2q4qh 124 36 , , , cord-320015-lbr2q4qh 124 37 87 87 CD cord-320015-lbr2q4qh 124 38 ] ] -RRB- cord-320015-lbr2q4qh 124 39 . . . cord-320015-lbr2q4qh 125 1 Phylogenetic phylogenetic JJ cord-320015-lbr2q4qh 125 2 analysis analysis NN cord-320015-lbr2q4qh 125 3 using use VBG cord-320015-lbr2q4qh 125 4 the the DT cord-320015-lbr2q4qh 125 5 26 26 CD cord-320015-lbr2q4qh 125 6 core core NN cord-320015-lbr2q4qh 125 7 iridovirid iridovirid NN cord-320015-lbr2q4qh 125 8 genes gene NNS cord-320015-lbr2q4qh 125 9 identified identify VBN cord-320015-lbr2q4qh 125 10 by by IN cord-320015-lbr2q4qh 125 11 Eaton Eaton NNP cord-320015-lbr2q4qh 125 12 and and CC cord-320015-lbr2q4qh 125 13 her -PRON- PRP$ cord-320015-lbr2q4qh 125 14 co co NNS cord-320015-lbr2q4qh 125 15 - - NNS cord-320015-lbr2q4qh 125 16 workers worker NNS cord-320015-lbr2q4qh 125 17 [ [ -LRB- cord-320015-lbr2q4qh 125 18 11 11 CD cord-320015-lbr2q4qh 125 19 ] ] -RRB- cord-320015-lbr2q4qh 125 20 support support VBP cord-320015-lbr2q4qh 125 21 whole whole JJ cord-320015-lbr2q4qh 125 22 genome genome NN cord-320015-lbr2q4qh 125 23 dot dot NN cord-320015-lbr2q4qh 125 24 plot plot NN cord-320015-lbr2q4qh 125 25 analysis analysis NN cord-320015-lbr2q4qh 125 26 and and CC cord-320015-lbr2q4qh 125 27 indicate indicate VBP cord-320015-lbr2q4qh 125 28 that that IN cord-320015-lbr2q4qh 125 29 FV3-like fv3-like IN cord-320015-lbr2q4qh 125 30 viruses virus NNS cord-320015-lbr2q4qh 125 31 ( ( -LRB- cord-320015-lbr2q4qh 125 32 FV3 FV3 NNP cord-320015-lbr2q4qh 125 33 , , , cord-320015-lbr2q4qh 125 34 TFV TFV NNP cord-320015-lbr2q4qh 125 35 , , , cord-320015-lbr2q4qh 125 36 STIV STIV NNP cord-320015-lbr2q4qh 125 37 ) ) -RRB- cord-320015-lbr2q4qh 125 38 , , , cord-320015-lbr2q4qh 125 39 ATV ATV NNP cord-320015-lbr2q4qh 125 40 - - HYPH cord-320015-lbr2q4qh 125 41 like like JJ cord-320015-lbr2q4qh 125 42 viruses virus NNS cord-320015-lbr2q4qh 125 43 ( ( -LRB- cord-320015-lbr2q4qh 125 44 ATV ATV NNP cord-320015-lbr2q4qh 125 45 and and CC cord-320015-lbr2q4qh 125 46 EHNV EHNV NNP cord-320015-lbr2q4qh 125 47 ) ) -RRB- cord-320015-lbr2q4qh 125 48 , , , cord-320015-lbr2q4qh 125 49 and and CC cord-320015-lbr2q4qh 125 50 GIV giv NN cord-320015-lbr2q4qh 125 51 - - HYPH cord-320015-lbr2q4qh 125 52 like like JJ cord-320015-lbr2q4qh 125 53 viruses virus NNS cord-320015-lbr2q4qh 125 54 ( ( -LRB- cord-320015-lbr2q4qh 125 55 SGIV SGIV NNP cord-320015-lbr2q4qh 125 56 and and CC cord-320015-lbr2q4qh 125 57 GIV GIV NNP cord-320015-lbr2q4qh 125 58 ) ) -RRB- cord-320015-lbr2q4qh 125 59 cluster cluster NN cord-320015-lbr2q4qh 125 60 on on IN cord-320015-lbr2q4qh 125 61 separate separate JJ cord-320015-lbr2q4qh 125 62 branches branch NNS cord-320015-lbr2q4qh 125 63 of of IN cord-320015-lbr2q4qh 125 64 the the DT cord-320015-lbr2q4qh 125 65 ranavirus ranavirus NNP cord-320015-lbr2q4qh 125 66 tree tree NN cord-320015-lbr2q4qh 125 67 ( ( -LRB- cord-320015-lbr2q4qh 125 68 Figure figure NN cord-320015-lbr2q4qh 125 69 3 3 CD cord-320015-lbr2q4qh 125 70 ) ) -RRB- cord-320015-lbr2q4qh 125 71 . . . cord-320015-lbr2q4qh 126 1 Collectively collectively RB cord-320015-lbr2q4qh 126 2 , , , cord-320015-lbr2q4qh 126 3 these these DT cord-320015-lbr2q4qh 126 4 data datum NNS cord-320015-lbr2q4qh 126 5 suggest suggest VBP cord-320015-lbr2q4qh 126 6 that that IN cord-320015-lbr2q4qh 126 7 for for IN cord-320015-lbr2q4qh 126 8 the the DT cord-320015-lbr2q4qh 126 9 greater great JJR cord-320015-lbr2q4qh 126 10 part part NN cord-320015-lbr2q4qh 126 11 of of IN cord-320015-lbr2q4qh 126 12 their -PRON- PRP$ cord-320015-lbr2q4qh 126 13 evolutionary evolutionary JJ cord-320015-lbr2q4qh 126 14 history history NN cord-320015-lbr2q4qh 126 15 , , , cord-320015-lbr2q4qh 126 16 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 126 17 were be VBD cord-320015-lbr2q4qh 126 18 restricted restrict VBN cord-320015-lbr2q4qh 126 19 to to IN cord-320015-lbr2q4qh 126 20 infecting infect VBG cord-320015-lbr2q4qh 126 21 fish fish NN cord-320015-lbr2q4qh 126 22 . . . cord-320015-lbr2q4qh 127 1 In in IN cord-320015-lbr2q4qh 127 2 addition addition NN cord-320015-lbr2q4qh 127 3 , , , cord-320015-lbr2q4qh 127 4 the the DT cord-320015-lbr2q4qh 127 5 shallow shallow JJ cord-320015-lbr2q4qh 127 6 branch branch NN cord-320015-lbr2q4qh 127 7 lengths length NNS cord-320015-lbr2q4qh 127 8 of of IN cord-320015-lbr2q4qh 127 9 FV3-like fv3-like NN cord-320015-lbr2q4qh 127 10 and and CC cord-320015-lbr2q4qh 127 11 ATV ATV NNP cord-320015-lbr2q4qh 127 12 - - HYPH cord-320015-lbr2q4qh 127 13 like like JJ cord-320015-lbr2q4qh 127 14 viruses virus NNS cord-320015-lbr2q4qh 127 15 , , , cord-320015-lbr2q4qh 127 16 which which WDT cord-320015-lbr2q4qh 127 17 infect infect VBP cord-320015-lbr2q4qh 127 18 amphibians amphibian NNS cord-320015-lbr2q4qh 127 19 and and CC cord-320015-lbr2q4qh 127 20 fish fish NN cord-320015-lbr2q4qh 127 21 , , , cord-320015-lbr2q4qh 127 22 suggest suggest VBP cord-320015-lbr2q4qh 127 23 that that IN cord-320015-lbr2q4qh 127 24 this this DT cord-320015-lbr2q4qh 127 25 group group NN cord-320015-lbr2q4qh 127 26 of of IN cord-320015-lbr2q4qh 127 27 viruses virus NNS cord-320015-lbr2q4qh 127 28 has have VBZ cord-320015-lbr2q4qh 127 29 recently recently RB cord-320015-lbr2q4qh 127 30 , , , cord-320015-lbr2q4qh 127 31 at at IN cord-320015-lbr2q4qh 127 32 least least JJS cord-320015-lbr2q4qh 127 33 in in IN cord-320015-lbr2q4qh 127 34 evolutionary evolutionary JJ cord-320015-lbr2q4qh 127 35 terms term NNS cord-320015-lbr2q4qh 127 36 , , , cord-320015-lbr2q4qh 127 37 radiated radiate VBD cord-320015-lbr2q4qh 127 38 to to TO cord-320015-lbr2q4qh 127 39 infect infect VB cord-320015-lbr2q4qh 127 40 a a DT cord-320015-lbr2q4qh 127 41 wide wide JJ cord-320015-lbr2q4qh 127 42 - - HYPH cord-320015-lbr2q4qh 127 43 range range NN cord-320015-lbr2q4qh 127 44 of of IN cord-320015-lbr2q4qh 127 45 poikilothermic poikilothermic JJ cord-320015-lbr2q4qh 127 46 vertebrates vertebrate NNS cord-320015-lbr2q4qh 127 47 [ [ -LRB- cord-320015-lbr2q4qh 127 48 12 12 CD cord-320015-lbr2q4qh 127 49 ] ] -RRB- cord-320015-lbr2q4qh 127 50 . . . cord-320015-lbr2q4qh 128 1 This this DT cord-320015-lbr2q4qh 128 2 hypothesis hypothesis NN cord-320015-lbr2q4qh 128 3 is be VBZ cord-320015-lbr2q4qh 128 4 supported support VBN cord-320015-lbr2q4qh 128 5 by by IN cord-320015-lbr2q4qh 128 6 studies study NNS cord-320015-lbr2q4qh 128 7 showing show VBG cord-320015-lbr2q4qh 128 8 that that IN cord-320015-lbr2q4qh 128 9 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 128 10 are be VBP cord-320015-lbr2q4qh 128 11 multi multi JJ cord-320015-lbr2q4qh 128 12 - - JJ cord-320015-lbr2q4qh 128 13 host host JJ cord-320015-lbr2q4qh 128 14 pathogens pathogen NNS cord-320015-lbr2q4qh 128 15 [ [ -LRB- cord-320015-lbr2q4qh 128 16 88 88 CD cord-320015-lbr2q4qh 128 17 ] ] -RRB- cord-320015-lbr2q4qh 128 18 . . . cord-320015-lbr2q4qh 129 1 As as IN cord-320015-lbr2q4qh 129 2 additional additional JJ cord-320015-lbr2q4qh 129 3 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 129 4 are be VBP cord-320015-lbr2q4qh 129 5 sequenced sequence VBN cord-320015-lbr2q4qh 129 6 , , , cord-320015-lbr2q4qh 129 7 particularly particularly RB cord-320015-lbr2q4qh 129 8 those those DT cord-320015-lbr2q4qh 129 9 isolates isolate NNS cord-320015-lbr2q4qh 129 10 that that WDT cord-320015-lbr2q4qh 129 11 infect infect VBP cord-320015-lbr2q4qh 129 12 multiple multiple JJ cord-320015-lbr2q4qh 129 13 host host NN cord-320015-lbr2q4qh 129 14 species specie NNS cord-320015-lbr2q4qh 129 15 , , , cord-320015-lbr2q4qh 129 16 a a DT cord-320015-lbr2q4qh 129 17 better well JJR cord-320015-lbr2q4qh 129 18 understanding understanding NN cord-320015-lbr2q4qh 129 19 of of IN cord-320015-lbr2q4qh 129 20 ranavirus ranavirus NNP cord-320015-lbr2q4qh 129 21 evolution evolution NN cord-320015-lbr2q4qh 129 22 , , , cord-320015-lbr2q4qh 129 23 host host NN cord-320015-lbr2q4qh 129 24 shifts shift NNS cord-320015-lbr2q4qh 129 25 , , , cord-320015-lbr2q4qh 129 26 and and CC cord-320015-lbr2q4qh 129 27 the the DT cord-320015-lbr2q4qh 129 28 molecular molecular JJ cord-320015-lbr2q4qh 129 29 determinants determinant NNS cord-320015-lbr2q4qh 129 30 of of IN cord-320015-lbr2q4qh 129 31 ranavirus ranavirus NNP cord-320015-lbr2q4qh 129 32 host host NN cord-320015-lbr2q4qh 129 33 range range NN cord-320015-lbr2q4qh 129 34 and and CC cord-320015-lbr2q4qh 129 35 pathogenesis pathogenesis NN cord-320015-lbr2q4qh 129 36 will will MD cord-320015-lbr2q4qh 129 37 be be VB cord-320015-lbr2q4qh 129 38 achieved achieve VBN cord-320015-lbr2q4qh 129 39 . . . cord-320015-lbr2q4qh 129 40 _SP cord-320015-lbr2q4qh 130 1 Pathological pathological JJ cord-320015-lbr2q4qh 130 2 and and CC cord-320015-lbr2q4qh 130 3 immunological immunological JJ cord-320015-lbr2q4qh 130 4 aspects aspect NNS cord-320015-lbr2q4qh 130 5 of of IN cord-320015-lbr2q4qh 130 6 infection infection NN cord-320015-lbr2q4qh 130 7 by by IN cord-320015-lbr2q4qh 130 8 FV3 FV3 NNP cord-320015-lbr2q4qh 130 9 and and CC cord-320015-lbr2q4qh 130 10 various various JJ cord-320015-lbr2q4qh 130 11 iridovirids iridovirid NNS cord-320015-lbr2q4qh 130 12 will will MD cord-320015-lbr2q4qh 130 13 be be VB cord-320015-lbr2q4qh 130 14 dealt deal VBN cord-320015-lbr2q4qh 130 15 with with IN cord-320015-lbr2q4qh 130 16 by by IN cord-320015-lbr2q4qh 130 17 Chen Chen NNP cord-320015-lbr2q4qh 130 18 and and CC cord-320015-lbr2q4qh 130 19 Robert Robert NNP cord-320015-lbr2q4qh 130 20 [ [ -LRB- cord-320015-lbr2q4qh 130 21 90 90 CD cord-320015-lbr2q4qh 130 22 ] ] -RRB- cord-320015-lbr2q4qh 130 23 and and CC cord-320015-lbr2q4qh 130 24 Miller Miller NNP cord-320015-lbr2q4qh 130 25 et et NNP cord-320015-lbr2q4qh 130 26 al al NNP cord-320015-lbr2q4qh 130 27 . . . cord-320015-lbr2q4qh 131 1 [ [ -LRB- cord-320015-lbr2q4qh 131 2 91 91 CD cord-320015-lbr2q4qh 131 3 ] ] -RRB- cord-320015-lbr2q4qh 131 4 in in IN cord-320015-lbr2q4qh 131 5 this this DT cord-320015-lbr2q4qh 131 6 special special JJ cord-320015-lbr2q4qh 131 7 issue issue NN cord-320015-lbr2q4qh 131 8 of of IN cord-320015-lbr2q4qh 131 9 Viruses virus NNS cord-320015-lbr2q4qh 131 10 . . . cord-320015-lbr2q4qh 132 1 Thus thus RB cord-320015-lbr2q4qh 132 2 , , , cord-320015-lbr2q4qh 132 3 only only RB cord-320015-lbr2q4qh 132 4 a a DT cord-320015-lbr2q4qh 132 5 brief brief JJ cord-320015-lbr2q4qh 132 6 overview overview NN cord-320015-lbr2q4qh 132 7 of of IN cord-320015-lbr2q4qh 132 8 this this DT cord-320015-lbr2q4qh 132 9 topic topic NN cord-320015-lbr2q4qh 132 10 will will MD cord-320015-lbr2q4qh 132 11 be be VB cord-320015-lbr2q4qh 132 12 provided provide VBN cord-320015-lbr2q4qh 132 13 here here RB cord-320015-lbr2q4qh 132 14 . . . cord-320015-lbr2q4qh 133 1 Although although IN cord-320015-lbr2q4qh 133 2 iridovirids iridovirid NNS cord-320015-lbr2q4qh 133 3 , , , cord-320015-lbr2q4qh 133 4 in in IN cord-320015-lbr2q4qh 133 5 contrast contrast NN cord-320015-lbr2q4qh 133 6 to to IN cord-320015-lbr2q4qh 133 7 the the DT cord-320015-lbr2q4qh 133 8 chytrid chytrid NN cord-320015-lbr2q4qh 133 9 fungus fungus NN cord-320015-lbr2q4qh 133 10 Batrachochytrium Batrachochytrium NNP cord-320015-lbr2q4qh 133 11 dendrobatidis dendrobatidis NNP cord-320015-lbr2q4qh 133 12 ( ( -LRB- cord-320015-lbr2q4qh 133 13 Bd Bd NNP cord-320015-lbr2q4qh 133 14 ) ) -RRB- cord-320015-lbr2q4qh 133 15 , , , cord-320015-lbr2q4qh 133 16 have have VBP cord-320015-lbr2q4qh 133 17 not not RB cord-320015-lbr2q4qh 133 18 been be VBN cord-320015-lbr2q4qh 133 19 reported report VBN cord-320015-lbr2q4qh 133 20 to to TO cord-320015-lbr2q4qh 133 21 drive drive VB cord-320015-lbr2q4qh 133 22 species specie NNS cord-320015-lbr2q4qh 133 23 extinction extinction NN cord-320015-lbr2q4qh 133 24 , , , cord-320015-lbr2q4qh 133 25 FV3-like fv3-like NN cord-320015-lbr2q4qh 133 26 viruses virus NNS cord-320015-lbr2q4qh 133 27 have have VBP cord-320015-lbr2q4qh 133 28 caused cause VBN cord-320015-lbr2q4qh 133 29 deaths death NNS cord-320015-lbr2q4qh 133 30 in in IN cord-320015-lbr2q4qh 133 31 several several JJ cord-320015-lbr2q4qh 133 32 amphibian amphibian NN cord-320015-lbr2q4qh 133 33 culture culture NN cord-320015-lbr2q4qh 133 34 facilities facility NNS cord-320015-lbr2q4qh 133 35 and and CC cord-320015-lbr2q4qh 133 36 in in IN cord-320015-lbr2q4qh 133 37 nature nature NN cord-320015-lbr2q4qh 133 38 , , , cord-320015-lbr2q4qh 133 39 and and CC cord-320015-lbr2q4qh 133 40 megalocytiviruses megalocytiviruse NNS cord-320015-lbr2q4qh 133 41 have have VBP cord-320015-lbr2q4qh 133 42 triggered trigger VBN cord-320015-lbr2q4qh 133 43 wide wide JJ cord-320015-lbr2q4qh 133 44 - - HYPH cord-320015-lbr2q4qh 133 45 spread spread NN cord-320015-lbr2q4qh 133 46 mortality mortality NN cord-320015-lbr2q4qh 133 47 in in IN cord-320015-lbr2q4qh 133 48 mariculture mariculture JJ cord-320015-lbr2q4qh 133 49 operations operation NNS cord-320015-lbr2q4qh 133 50 in in IN cord-320015-lbr2q4qh 133 51 south south JJ cord-320015-lbr2q4qh 133 52 - - HYPH cord-320015-lbr2q4qh 133 53 east east NN cord-320015-lbr2q4qh 133 54 Asia Asia NNP cord-320015-lbr2q4qh 133 55 [ [ -LRB- cord-320015-lbr2q4qh 133 56 1 1 CD cord-320015-lbr2q4qh 133 57 , , , cord-320015-lbr2q4qh 133 58 2 2 CD cord-320015-lbr2q4qh 133 59 , , , cord-320015-lbr2q4qh 133 60 [ [ -LRB- cord-320015-lbr2q4qh 133 61 92 92 CD cord-320015-lbr2q4qh 133 62 ] ] -RRB- cord-320015-lbr2q4qh 133 63 [ [ -LRB- cord-320015-lbr2q4qh 133 64 93 93 CD cord-320015-lbr2q4qh 133 65 ] ] -RRB- cord-320015-lbr2q4qh 133 66 [ [ -LRB- cord-320015-lbr2q4qh 133 67 94 94 CD cord-320015-lbr2q4qh 133 68 ] ] -RRB- cord-320015-lbr2q4qh 133 69 [ [ -LRB- cord-320015-lbr2q4qh 133 70 95 95 CD cord-320015-lbr2q4qh 133 71 ] ] -RRB- cord-320015-lbr2q4qh 133 72 . . . cord-320015-lbr2q4qh 134 1 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 134 2 infections infection NNS cord-320015-lbr2q4qh 134 3 range range VBP cord-320015-lbr2q4qh 134 4 from from IN cord-320015-lbr2q4qh 134 5 inapparent inapparent JJ cord-320015-lbr2q4qh 134 6 to to IN cord-320015-lbr2q4qh 134 7 fulminant fulminant JJ cord-320015-lbr2q4qh 134 8 and and CC cord-320015-lbr2q4qh 134 9 involve involve VB cord-320015-lbr2q4qh 134 10 a a DT cord-320015-lbr2q4qh 134 11 variety variety NN cord-320015-lbr2q4qh 134 12 of of IN cord-320015-lbr2q4qh 134 13 tissues tissue NNS cord-320015-lbr2q4qh 134 14 including include VBG cord-320015-lbr2q4qh 134 15 the the DT cord-320015-lbr2q4qh 134 16 skin skin NN cord-320015-lbr2q4qh 134 17 , , , cord-320015-lbr2q4qh 134 18 kidney kidney NN cord-320015-lbr2q4qh 134 19 , , , cord-320015-lbr2q4qh 134 20 liver liver NN cord-320015-lbr2q4qh 134 21 , , , cord-320015-lbr2q4qh 134 22 and and CC cord-320015-lbr2q4qh 134 23 intestine intestine NN cord-320015-lbr2q4qh 134 24 [ [ -LRB- cord-320015-lbr2q4qh 134 25 96 96 CD cord-320015-lbr2q4qh 134 26 ] ] -RRB- cord-320015-lbr2q4qh 134 27 . . . cord-320015-lbr2q4qh 135 1 Using use VBG cord-320015-lbr2q4qh 135 2 Xenopus Xenopus NNP cord-320015-lbr2q4qh 135 3 laevis laevi NNS cord-320015-lbr2q4qh 135 4 as as IN cord-320015-lbr2q4qh 135 5 a a DT cord-320015-lbr2q4qh 135 6 model model NN cord-320015-lbr2q4qh 135 7 host host NN cord-320015-lbr2q4qh 135 8 , , , cord-320015-lbr2q4qh 135 9 Robert Robert NNP cord-320015-lbr2q4qh 135 10 and and CC cord-320015-lbr2q4qh 135 11 his -PRON- PRP$ cord-320015-lbr2q4qh 135 12 colleagues colleague NNS cord-320015-lbr2q4qh 135 13 have have VBP cord-320015-lbr2q4qh 135 14 shown show VBN cord-320015-lbr2q4qh 135 15 that that IN cord-320015-lbr2q4qh 135 16 FV3 FV3 NNP cord-320015-lbr2q4qh 135 17 infection infection NN cord-320015-lbr2q4qh 135 18 occurs occur VBZ cord-320015-lbr2q4qh 135 19 in in IN cord-320015-lbr2q4qh 135 20 both both DT cord-320015-lbr2q4qh 135 21 larval larval JJ cord-320015-lbr2q4qh 135 22 and and CC cord-320015-lbr2q4qh 135 23 adult adult NN cord-320015-lbr2q4qh 135 24 animals animal NNS cord-320015-lbr2q4qh 135 25 [ [ -LRB- cord-320015-lbr2q4qh 135 26 96 96 CD cord-320015-lbr2q4qh 135 27 ] ] -RRB- cord-320015-lbr2q4qh 136 1 [ [ -LRB- cord-320015-lbr2q4qh 136 2 97 97 CD cord-320015-lbr2q4qh 136 3 ] ] -RRB- cord-320015-lbr2q4qh 136 4 [ [ -LRB- cord-320015-lbr2q4qh 136 5 98 98 CD cord-320015-lbr2q4qh 136 6 ] ] -RRB- cord-320015-lbr2q4qh 137 1 [ [ -LRB- cord-320015-lbr2q4qh 137 2 99 99 CD cord-320015-lbr2q4qh 137 3 ] ] -RRB- cord-320015-lbr2q4qh 137 4 [ [ -LRB- cord-320015-lbr2q4qh 137 5 100 100 CD cord-320015-lbr2q4qh 137 6 ] ] -RRB- cord-320015-lbr2q4qh 137 7 [ [ -LRB- cord-320015-lbr2q4qh 137 8 101 101 CD cord-320015-lbr2q4qh 137 9 ] ] -RRB- cord-320015-lbr2q4qh 137 10 [ [ -LRB- cord-320015-lbr2q4qh 137 11 102 102 CD cord-320015-lbr2q4qh 137 12 ] ] -RRB- cord-320015-lbr2q4qh 137 13 . . . cord-320015-lbr2q4qh 138 1 However however RB cord-320015-lbr2q4qh 138 2 , , , cord-320015-lbr2q4qh 138 3 whereas whereas IN cord-320015-lbr2q4qh 138 4 infections infection NNS cord-320015-lbr2q4qh 138 5 of of IN cord-320015-lbr2q4qh 138 6 immunocompetent immunocompetent JJ cord-320015-lbr2q4qh 138 7 adult adult NN cord-320015-lbr2q4qh 138 8 frogs frog NNS cord-320015-lbr2q4qh 138 9 are be VBP cord-320015-lbr2q4qh 138 10 confined confine VBN cord-320015-lbr2q4qh 138 11 to to IN cord-320015-lbr2q4qh 138 12 the the DT cord-320015-lbr2q4qh 138 13 kidney kidney NN cord-320015-lbr2q4qh 138 14 and and CC cord-320015-lbr2q4qh 138 15 cleared clear VBD cord-320015-lbr2q4qh 138 16 within within IN cord-320015-lbr2q4qh 138 17 a a DT cord-320015-lbr2q4qh 138 18 few few JJ cord-320015-lbr2q4qh 138 19 weeks week NNS cord-320015-lbr2q4qh 138 20 , , , cord-320015-lbr2q4qh 138 21 infection infection NN cord-320015-lbr2q4qh 138 22 of of IN cord-320015-lbr2q4qh 138 23 tadpoles tadpole NNS cord-320015-lbr2q4qh 138 24 or or CC cord-320015-lbr2q4qh 138 25 immunocompromised immunocompromised JJ cord-320015-lbr2q4qh 138 26 adults adult NNS cord-320015-lbr2q4qh 138 27 results result VBZ cord-320015-lbr2q4qh 138 28 in in IN cord-320015-lbr2q4qh 138 29 widespread widespread JJ cord-320015-lbr2q4qh 138 30 infection infection NN cord-320015-lbr2q4qh 138 31 that that WDT cord-320015-lbr2q4qh 138 32 spreads spread VBZ cord-320015-lbr2q4qh 138 33 to to IN cord-320015-lbr2q4qh 138 34 multiple multiple JJ cord-320015-lbr2q4qh 138 35 internal internal JJ cord-320015-lbr2q4qh 138 36 organs organ NNS cord-320015-lbr2q4qh 138 37 and and CC cord-320015-lbr2q4qh 138 38 often often RB cord-320015-lbr2q4qh 138 39 leads lead VBZ cord-320015-lbr2q4qh 138 40 to to IN cord-320015-lbr2q4qh 138 41 death death NN cord-320015-lbr2q4qh 138 42 . . . cord-320015-lbr2q4qh 139 1 Protection protection NN cord-320015-lbr2q4qh 139 2 from from IN cord-320015-lbr2q4qh 139 3 FV3 FV3 NNP cord-320015-lbr2q4qh 139 4 infection infection NN cord-320015-lbr2q4qh 139 5 appears appear VBZ cord-320015-lbr2q4qh 139 6 to to TO cord-320015-lbr2q4qh 139 7 involve involve VB cord-320015-lbr2q4qh 139 8 both both DT cord-320015-lbr2q4qh 139 9 humoral humoral JJ cord-320015-lbr2q4qh 139 10 and and CC cord-320015-lbr2q4qh 139 11 cell cell NN cord-320015-lbr2q4qh 139 12 - - HYPH cord-320015-lbr2q4qh 139 13 mediated mediate VBN cord-320015-lbr2q4qh 139 14 immunity immunity NN cord-320015-lbr2q4qh 139 15 and and CC cord-320015-lbr2q4qh 139 16 both both DT cord-320015-lbr2q4qh 139 17 antiviral antiviral JJ cord-320015-lbr2q4qh 139 18 antibodies antibody NNS cord-320015-lbr2q4qh 139 19 and and CC cord-320015-lbr2q4qh 139 20 cytotoxic cytotoxic JJ cord-320015-lbr2q4qh 139 21 T t NN cord-320015-lbr2q4qh 139 22 cells cell NNS cord-320015-lbr2q4qh 139 23 have have VBP cord-320015-lbr2q4qh 139 24 been be VBN cord-320015-lbr2q4qh 139 25 identified identify VBN cord-320015-lbr2q4qh 139 26 as as IN cord-320015-lbr2q4qh 139 27 protective protective JJ cord-320015-lbr2q4qh 139 28 . . . cord-320015-lbr2q4qh 140 1 Vaccination vaccination NN cord-320015-lbr2q4qh 140 2 may may MD cord-320015-lbr2q4qh 140 3 prove prove VB cord-320015-lbr2q4qh 140 4 useful useful JJ cord-320015-lbr2q4qh 140 5 in in IN cord-320015-lbr2q4qh 140 6 protecting protect VBG cord-320015-lbr2q4qh 140 7 susceptible susceptible JJ cord-320015-lbr2q4qh 140 8 species specie NNS cord-320015-lbr2q4qh 140 9 and and CC cord-320015-lbr2q4qh 140 10 vaccines vaccine NNS cord-320015-lbr2q4qh 140 11 have have VBP cord-320015-lbr2q4qh 140 12 been be VBN cord-320015-lbr2q4qh 140 13 developed develop VBN cord-320015-lbr2q4qh 140 14 to to TO cord-320015-lbr2q4qh 140 15 protect protect VB cord-320015-lbr2q4qh 140 16 commercially commercially RB cord-320015-lbr2q4qh 140 17 important important JJ cord-320015-lbr2q4qh 140 18 fish fish NN cord-320015-lbr2q4qh 140 19 from from IN cord-320015-lbr2q4qh 140 20 megalocytivirus megalocytivirus NNP cord-320015-lbr2q4qh 140 21 infection infection NN cord-320015-lbr2q4qh 140 22 [ [ -LRB- cord-320015-lbr2q4qh 140 23 103 103 CD cord-320015-lbr2q4qh 140 24 , , , cord-320015-lbr2q4qh 140 25 104 104 CD cord-320015-lbr2q4qh 140 26 ] ] -RRB- cord-320015-lbr2q4qh 140 27 . . . cord-320015-lbr2q4qh 141 1 Likewise likewise RB cord-320015-lbr2q4qh 141 2 , , , cord-320015-lbr2q4qh 141 3 infection infection NN cord-320015-lbr2q4qh 141 4 of of IN cord-320015-lbr2q4qh 141 5 bullfrog bullfrog NN cord-320015-lbr2q4qh 141 6 tadpoles tadpole NNS cord-320015-lbr2q4qh 141 7 ( ( -LRB- cord-320015-lbr2q4qh 141 8 Rana Rana NNP cord-320015-lbr2q4qh 141 9 catesbeiana catesbeiana NNP cord-320015-lbr2q4qh 141 10 ) ) -RRB- cord-320015-lbr2q4qh 141 11 with with IN cord-320015-lbr2q4qh 141 12 less less RBR cord-320015-lbr2q4qh 141 13 pathogenic pathogenic JJ cord-320015-lbr2q4qh 141 14 FV3 FV3 NNP cord-320015-lbr2q4qh 141 15 protected protect VBD cord-320015-lbr2q4qh 141 16 them -PRON- PRP cord-320015-lbr2q4qh 141 17 against against IN cord-320015-lbr2q4qh 141 18 death death NN cord-320015-lbr2q4qh 141 19 following follow VBG cord-320015-lbr2q4qh 141 20 infection infection NN cord-320015-lbr2q4qh 141 21 with with IN cord-320015-lbr2q4qh 141 22 the the DT cord-320015-lbr2q4qh 141 23 more more RBR cord-320015-lbr2q4qh 141 24 pathogenic pathogenic JJ cord-320015-lbr2q4qh 141 25 Rana Rana NNP cord-320015-lbr2q4qh 141 26 catesbeiana catesbeiana NN cord-320015-lbr2q4qh 141 27 virus virus NN cord-320015-lbr2q4qh 141 28 Z Z NNP cord-320015-lbr2q4qh 141 29 [ [ -LRB- cord-320015-lbr2q4qh 141 30 63 63 CD cord-320015-lbr2q4qh 141 31 ] ] -RRB- cord-320015-lbr2q4qh 141 32 . . . cord-320015-lbr2q4qh 142 1 As as IN cord-320015-lbr2q4qh 142 2 with with IN cord-320015-lbr2q4qh 142 3 the the DT cord-320015-lbr2q4qh 142 4 above above JJ cord-320015-lbr2q4qh 142 5 section section NN cord-320015-lbr2q4qh 142 6 , , , cord-320015-lbr2q4qh 142 7 the the DT cord-320015-lbr2q4qh 142 8 ecology ecology NN cord-320015-lbr2q4qh 142 9 of of IN cord-320015-lbr2q4qh 142 10 ranavirus ranavirus NNP cord-320015-lbr2q4qh 142 11 infections infection NNS cord-320015-lbr2q4qh 142 12 will will MD cord-320015-lbr2q4qh 142 13 be be VB cord-320015-lbr2q4qh 142 14 more more RBR cord-320015-lbr2q4qh 142 15 fully fully RB cord-320015-lbr2q4qh 142 16 discussed discuss VBN cord-320015-lbr2q4qh 142 17 by by IN cord-320015-lbr2q4qh 142 18 Miller Miller NNP cord-320015-lbr2q4qh 142 19 and and CC cord-320015-lbr2q4qh 142 20 co co NNS cord-320015-lbr2q4qh 142 21 - - NNS cord-320015-lbr2q4qh 142 22 workers worker NNS cord-320015-lbr2q4qh 142 23 in in IN cord-320015-lbr2q4qh 142 24 their -PRON- PRP$ cord-320015-lbr2q4qh 142 25 contribution contribution NN cord-320015-lbr2q4qh 142 26 to to IN cord-320015-lbr2q4qh 142 27 this this DT cord-320015-lbr2q4qh 142 28 issue issue NN cord-320015-lbr2q4qh 142 29 [ [ -LRB- cord-320015-lbr2q4qh 142 30 91 91 CD cord-320015-lbr2q4qh 142 31 ] ] -RRB- cord-320015-lbr2q4qh 142 32 . . . cord-320015-lbr2q4qh 143 1 Suffice suffice VB cord-320015-lbr2q4qh 143 2 it -PRON- PRP cord-320015-lbr2q4qh 143 3 to to TO cord-320015-lbr2q4qh 143 4 say say VB cord-320015-lbr2q4qh 143 5 iridovirus iridovirus NNP cord-320015-lbr2q4qh 143 6 infections infection NNS cord-320015-lbr2q4qh 143 7 have have VBP cord-320015-lbr2q4qh 143 8 a a DT cord-320015-lbr2q4qh 143 9 marked marked JJ cord-320015-lbr2q4qh 143 10 effect effect NN cord-320015-lbr2q4qh 143 11 on on IN cord-320015-lbr2q4qh 143 12 lower low JJR cord-320015-lbr2q4qh 143 13 vertebrates vertebrate NNS cord-320015-lbr2q4qh 143 14 . . . cord-320015-lbr2q4qh 144 1 As as IN cord-320015-lbr2q4qh 144 2 Green Green NNP cord-320015-lbr2q4qh 144 3 and and CC cord-320015-lbr2q4qh 144 4 co co NNS cord-320015-lbr2q4qh 144 5 - - NNS cord-320015-lbr2q4qh 144 6 workers worker NNS cord-320015-lbr2q4qh 144 7 observed observe VBD cord-320015-lbr2q4qh 144 8 most most JJS cord-320015-lbr2q4qh 144 9 mortality mortality NN cord-320015-lbr2q4qh 144 10 events event NNS cord-320015-lbr2q4qh 144 11 seen see VBN cord-320015-lbr2q4qh 144 12 among among IN cord-320015-lbr2q4qh 144 13 amphibians amphibian NNS cord-320015-lbr2q4qh 144 14 from from IN cord-320015-lbr2q4qh 144 15 1996 1996 CD cord-320015-lbr2q4qh 144 16 - - SYM cord-320015-lbr2q4qh 144 17 2001 2001 CD cord-320015-lbr2q4qh 144 18 were be VBD cord-320015-lbr2q4qh 144 19 attributable attributable JJ cord-320015-lbr2q4qh 144 20 to to IN cord-320015-lbr2q4qh 144 21 iridovirus iridovirus JJ cord-320015-lbr2q4qh 144 22 infections infection NNS cord-320015-lbr2q4qh 144 23 [ [ -LRB- cord-320015-lbr2q4qh 144 24 105 105 CD cord-320015-lbr2q4qh 144 25 ] ] -RRB- cord-320015-lbr2q4qh 144 26 . . . cord-320015-lbr2q4qh 145 1 In in IN cord-320015-lbr2q4qh 145 2 addition addition NN cord-320015-lbr2q4qh 145 3 , , , cord-320015-lbr2q4qh 145 4 whereas whereas IN cord-320015-lbr2q4qh 145 5 lymphocystivirus lymphocystivirus NNP cord-320015-lbr2q4qh 145 6 and and CC cord-320015-lbr2q4qh 145 7 megalocytivirus megalocytivirus NNP cord-320015-lbr2q4qh 145 8 infections infection NNS cord-320015-lbr2q4qh 145 9 have have VBP cord-320015-lbr2q4qh 145 10 , , , cord-320015-lbr2q4qh 145 11 to to IN cord-320015-lbr2q4qh 145 12 date date NN cord-320015-lbr2q4qh 145 13 , , , cord-320015-lbr2q4qh 145 14 only only RB cord-320015-lbr2q4qh 145 15 been be VBN cord-320015-lbr2q4qh 145 16 detected detect VBN cord-320015-lbr2q4qh 145 17 in in IN cord-320015-lbr2q4qh 145 18 fish fish NN cord-320015-lbr2q4qh 145 19 species specie NNS cord-320015-lbr2q4qh 145 20 , , , cord-320015-lbr2q4qh 145 21 ranavirus ranavirus NNP cord-320015-lbr2q4qh 145 22 infections infection NNS cord-320015-lbr2q4qh 145 23 affect affect VBP cord-320015-lbr2q4qh 145 24 three three CD cord-320015-lbr2q4qh 145 25 classes class NNS cord-320015-lbr2q4qh 145 26 of of IN cord-320015-lbr2q4qh 145 27 ectothermic ectothermic JJ cord-320015-lbr2q4qh 145 28 vertebrates vertebrate NNS cord-320015-lbr2q4qh 145 29 : : : cord-320015-lbr2q4qh 145 30 bony bony JJ cord-320015-lbr2q4qh 145 31 fish fish NN cord-320015-lbr2q4qh 145 32 , , , cord-320015-lbr2q4qh 145 33 amphibians amphibian NNS cord-320015-lbr2q4qh 145 34 , , , cord-320015-lbr2q4qh 145 35 and and CC cord-320015-lbr2q4qh 145 36 reptiles reptile NNS cord-320015-lbr2q4qh 145 37 [ [ -LRB- cord-320015-lbr2q4qh 145 38 2 2 CD cord-320015-lbr2q4qh 145 39 ] ] -RRB- cord-320015-lbr2q4qh 145 40 . . . cord-320015-lbr2q4qh 146 1 Results result NNS cord-320015-lbr2q4qh 146 2 from from IN cord-320015-lbr2q4qh 146 3 experimental experimental JJ cord-320015-lbr2q4qh 146 4 challenges challenge NNS cord-320015-lbr2q4qh 146 5 as as RB cord-320015-lbr2q4qh 146 6 well well RB cord-320015-lbr2q4qh 146 7 as as IN cord-320015-lbr2q4qh 146 8 natural natural JJ cord-320015-lbr2q4qh 146 9 infections infection NNS cord-320015-lbr2q4qh 146 10 suggest suggest VBP cord-320015-lbr2q4qh 146 11 that that IN cord-320015-lbr2q4qh 146 12 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 146 13 isolated isolate VBN cord-320015-lbr2q4qh 146 14 from from IN cord-320015-lbr2q4qh 146 15 one one CD cord-320015-lbr2q4qh 146 16 species specie NNS cord-320015-lbr2q4qh 146 17 can can MD cord-320015-lbr2q4qh 146 18 not not RB cord-320015-lbr2q4qh 146 19 only only RB cord-320015-lbr2q4qh 146 20 infect infect VB cord-320015-lbr2q4qh 146 21 different different JJ cord-320015-lbr2q4qh 146 22 host host NN cord-320015-lbr2q4qh 146 23 species specie NNS cord-320015-lbr2q4qh 146 24 within within IN cord-320015-lbr2q4qh 146 25 the the DT cord-320015-lbr2q4qh 146 26 same same JJ cord-320015-lbr2q4qh 146 27 genus genus NN cord-320015-lbr2q4qh 146 28 , , , cord-320015-lbr2q4qh 146 29 but but CC cord-320015-lbr2q4qh 146 30 also also RB cord-320015-lbr2q4qh 146 31 host host VBP cord-320015-lbr2q4qh 146 32 species specie NNS cord-320015-lbr2q4qh 146 33 from from IN cord-320015-lbr2q4qh 146 34 different different JJ cord-320015-lbr2q4qh 146 35 genera genus NNS cord-320015-lbr2q4qh 146 36 , , , cord-320015-lbr2q4qh 146 37 orders order NNS cord-320015-lbr2q4qh 146 38 , , , cord-320015-lbr2q4qh 146 39 and and CC cord-320015-lbr2q4qh 146 40 classes class NNS cord-320015-lbr2q4qh 146 41 . . . cord-320015-lbr2q4qh 147 1 For for IN cord-320015-lbr2q4qh 147 2 example example NN cord-320015-lbr2q4qh 147 3 , , , cord-320015-lbr2q4qh 147 4 amphibian amphibian NN cord-320015-lbr2q4qh 147 5 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 147 6 such such JJ cord-320015-lbr2q4qh 147 7 as as IN cord-320015-lbr2q4qh 147 8 Bohle Bohle NNP cord-320015-lbr2q4qh 147 9 iridovirus iridovirus NNP cord-320015-lbr2q4qh 147 10 , , , cord-320015-lbr2q4qh 147 11 FV3 FV3 NNP cord-320015-lbr2q4qh 147 12 , , , cord-320015-lbr2q4qh 147 13 and and CC cord-320015-lbr2q4qh 147 14 ATV ATV NNP cord-320015-lbr2q4qh 147 15 infect infect VBP cord-320015-lbr2q4qh 147 16 a a DT cord-320015-lbr2q4qh 147 17 variety variety NN cord-320015-lbr2q4qh 147 18 of of IN cord-320015-lbr2q4qh 147 19 fish fish NN cord-320015-lbr2q4qh 147 20 and and CC cord-320015-lbr2q4qh 147 21 amphibian amphibian NN cord-320015-lbr2q4qh 147 22 species specie NNS cord-320015-lbr2q4qh 147 23 , , , cord-320015-lbr2q4qh 147 24 and and CC cord-320015-lbr2q4qh 147 25 at at RB cord-320015-lbr2q4qh 147 26 least least RBS cord-320015-lbr2q4qh 147 27 one one CD cord-320015-lbr2q4qh 147 28 insect insect NN cord-320015-lbr2q4qh 147 29 virus virus NN cord-320015-lbr2q4qh 147 30 appears appear VBZ cord-320015-lbr2q4qh 147 31 to to TO cord-320015-lbr2q4qh 147 32 also also RB cord-320015-lbr2q4qh 147 33 infect infect VB cord-320015-lbr2q4qh 147 34 reptiles reptile NNS cord-320015-lbr2q4qh 147 35 [ [ -LRB- cord-320015-lbr2q4qh 147 36 9,10,88,106 9,10,88,106 NNP cord-320015-lbr2q4qh 147 37 - - HYPH cord-320015-lbr2q4qh 147 38 109 109 CD cord-320015-lbr2q4qh 147 39 ] ] -RRB- cord-320015-lbr2q4qh 147 40 . . . cord-320015-lbr2q4qh 148 1 Early early JJ cord-320015-lbr2q4qh 148 2 studies study NNS cord-320015-lbr2q4qh 148 3 with with IN cord-320015-lbr2q4qh 148 4 FV3 FV3 NNP cord-320015-lbr2q4qh 148 5 attempted attempt VBD cord-320015-lbr2q4qh 148 6 to to TO cord-320015-lbr2q4qh 148 7 elucidate elucidate VB cord-320015-lbr2q4qh 148 8 viral viral JJ cord-320015-lbr2q4qh 148 9 replicative replicative JJ cord-320015-lbr2q4qh 148 10 events event NNS cord-320015-lbr2q4qh 148 11 and and CC cord-320015-lbr2q4qh 148 12 the the DT cord-320015-lbr2q4qh 148 13 function function NN cord-320015-lbr2q4qh 148 14 of of IN cord-320015-lbr2q4qh 148 15 viral viral JJ cord-320015-lbr2q4qh 148 16 gene gene NN cord-320015-lbr2q4qh 148 17 products product NNS cord-320015-lbr2q4qh 148 18 using use VBG cord-320015-lbr2q4qh 148 19 a a DT cord-320015-lbr2q4qh 148 20 variety variety NN cord-320015-lbr2q4qh 148 21 of of IN cord-320015-lbr2q4qh 148 22 inhibitors inhibitor NNS cord-320015-lbr2q4qh 148 23 , , , cord-320015-lbr2q4qh 148 24 e.g. e.g. RB cord-320015-lbr2q4qh 148 25 , , , cord-320015-lbr2q4qh 148 26 cycloheximide cycloheximide NN cord-320015-lbr2q4qh 148 27 to to TO cord-320015-lbr2q4qh 148 28 confine confine VB cord-320015-lbr2q4qh 148 29 viral viral JJ cord-320015-lbr2q4qh 148 30 gene gene NN cord-320015-lbr2q4qh 148 31 expression expression NN cord-320015-lbr2q4qh 148 32 to to IN cord-320015-lbr2q4qh 148 33 IE IE NNP cord-320015-lbr2q4qh 148 34 transcription transcription NN cord-320015-lbr2q4qh 148 35 , , , cord-320015-lbr2q4qh 148 36 α α NN cord-320015-lbr2q4qh 148 37 - - HYPH cord-320015-lbr2q4qh 148 38 amanitin amanitin NNP cord-320015-lbr2q4qh 148 39 to to TO cord-320015-lbr2q4qh 148 40 block block VB cord-320015-lbr2q4qh 148 41 host host NN cord-320015-lbr2q4qh 148 42 RNA RNA NNP cord-320015-lbr2q4qh 148 43 polymerase polymerase NN cord-320015-lbr2q4qh 148 44 II II NNP cord-320015-lbr2q4qh 148 45 activity activity NN cord-320015-lbr2q4qh 148 46 , , , cord-320015-lbr2q4qh 148 47 phosphonacetic phosphonacetic JJ cord-320015-lbr2q4qh 148 48 acid acid NN cord-320015-lbr2q4qh 148 49 to to TO cord-320015-lbr2q4qh 148 50 block block VB cord-320015-lbr2q4qh 148 51 viral viral JJ cord-320015-lbr2q4qh 148 52 DNA dna NN cord-320015-lbr2q4qh 148 53 synthesis synthesis NN cord-320015-lbr2q4qh 148 54 , , , cord-320015-lbr2q4qh 148 55 azacytidine azacytidine NN cord-320015-lbr2q4qh 148 56 to to TO cord-320015-lbr2q4qh 148 57 block block VB cord-320015-lbr2q4qh 148 58 the the DT cord-320015-lbr2q4qh 148 59 methylation methylation NN cord-320015-lbr2q4qh 148 60 of of IN cord-320015-lbr2q4qh 148 61 viral viral JJ cord-320015-lbr2q4qh 148 62 DNA dna NN cord-320015-lbr2q4qh 148 63 , , , cord-320015-lbr2q4qh 148 64 flurophenylalanine flurophenylalanine NN cord-320015-lbr2q4qh 148 65 to to TO cord-320015-lbr2q4qh 148 66 block block VB cord-320015-lbr2q4qh 148 67 the the DT cord-320015-lbr2q4qh 148 68 expression expression NN cord-320015-lbr2q4qh 148 69 of of IN cord-320015-lbr2q4qh 148 70 late late JJ cord-320015-lbr2q4qh 148 71 viral viral JJ cord-320015-lbr2q4qh 148 72 proteins protein NNS cord-320015-lbr2q4qh 148 73 , , , cord-320015-lbr2q4qh 148 74 elevated elevated JJ cord-320015-lbr2q4qh 148 75 temperatures temperature NNS cord-320015-lbr2q4qh 148 76 ( ( -LRB- cord-320015-lbr2q4qh 148 77 37 37 CD cord-320015-lbr2q4qh 148 78 ° ° NN cord-320015-lbr2q4qh 148 79 C c NN cord-320015-lbr2q4qh 148 80 ) ) -RRB- cord-320015-lbr2q4qh 148 81 to to TO cord-320015-lbr2q4qh 148 82 confine confine VB cord-320015-lbr2q4qh 148 83 viral viral JJ cord-320015-lbr2q4qh 148 84 gene gene NN cord-320015-lbr2q4qh 148 85 expression expression NN cord-320015-lbr2q4qh 148 86 to to IN cord-320015-lbr2q4qh 148 87 early early JJ cord-320015-lbr2q4qh 148 88 transcripts transcript NNS cord-320015-lbr2q4qh 148 89 and and CC cord-320015-lbr2q4qh 148 90 proteins protein NNS cord-320015-lbr2q4qh 148 91 , , , cord-320015-lbr2q4qh 148 92 etc etc FW cord-320015-lbr2q4qh 148 93 . . . cord-320015-lbr2q4qh 149 1 ( ( -LRB- cord-320015-lbr2q4qh 149 2 reviewed review VBN cord-320015-lbr2q4qh 149 3 in in IN cord-320015-lbr2q4qh 149 4 [ [ -LRB- cord-320015-lbr2q4qh 149 5 110 110 CD cord-320015-lbr2q4qh 149 6 , , , cord-320015-lbr2q4qh 149 7 111 111 CD cord-320015-lbr2q4qh 149 8 ] ] -RRB- cord-320015-lbr2q4qh 149 9 ) ) -RRB- cord-320015-lbr2q4qh 149 10 . . . cord-320015-lbr2q4qh 150 1 In in IN cord-320015-lbr2q4qh 150 2 addition addition NN cord-320015-lbr2q4qh 150 3 , , , cord-320015-lbr2q4qh 150 4 panels panel NNS cord-320015-lbr2q4qh 150 5 of of IN cord-320015-lbr2q4qh 150 6 ts ts NNP cord-320015-lbr2q4qh 150 7 mutants mutant NNS cord-320015-lbr2q4qh 150 8 were be VBD cord-320015-lbr2q4qh 150 9 generated generate VBN cord-320015-lbr2q4qh 150 10 and and CC cord-320015-lbr2q4qh 150 11 subsequently subsequently RB cord-320015-lbr2q4qh 150 12 characterized characterize VBD cord-320015-lbr2q4qh 150 13 [ [ -LRB- cord-320015-lbr2q4qh 150 14 47 47 CD cord-320015-lbr2q4qh 150 15 , , , cord-320015-lbr2q4qh 150 16 112 112 CD cord-320015-lbr2q4qh 150 17 , , , cord-320015-lbr2q4qh 150 18 113 113 CD cord-320015-lbr2q4qh 150 19 ] ] -RRB- cord-320015-lbr2q4qh 150 20 . . . cord-320015-lbr2q4qh 151 1 Ts Ts NNP cord-320015-lbr2q4qh 151 2 mutants mutant NNS cord-320015-lbr2q4qh 151 3 proved prove VBD cord-320015-lbr2q4qh 151 4 useful useful JJ cord-320015-lbr2q4qh 151 5 in in IN cord-320015-lbr2q4qh 151 6 confirming confirm VBG cord-320015-lbr2q4qh 151 7 and and CC cord-320015-lbr2q4qh 151 8 identifying identify VBG cord-320015-lbr2q4qh 151 9 various various JJ cord-320015-lbr2q4qh 151 10 aspects aspect NNS cord-320015-lbr2q4qh 151 11 of of IN cord-320015-lbr2q4qh 151 12 the the DT cord-320015-lbr2q4qh 151 13 virus virus NN cord-320015-lbr2q4qh 151 14 life life NN cord-320015-lbr2q4qh 151 15 cycle cycle NN cord-320015-lbr2q4qh 151 16 , , , cord-320015-lbr2q4qh 151 17 e.g. e.g. RB cord-320015-lbr2q4qh 151 18 , , , cord-320015-lbr2q4qh 151 19 the the DT cord-320015-lbr2q4qh 151 20 two two CD cord-320015-lbr2q4qh 151 21 - - HYPH cord-320015-lbr2q4qh 151 22 stages stage NNS cord-320015-lbr2q4qh 151 23 of of IN cord-320015-lbr2q4qh 151 24 viral viral JJ cord-320015-lbr2q4qh 151 25 DNA dna NN cord-320015-lbr2q4qh 151 26 synthesis synthesis NN cord-320015-lbr2q4qh 151 27 [ [ -LRB- cord-320015-lbr2q4qh 151 28 114 114 CD cord-320015-lbr2q4qh 151 29 ] ] -RRB- cord-320015-lbr2q4qh 151 30 . . . cord-320015-lbr2q4qh 152 1 Ultimately ultimately RB cord-320015-lbr2q4qh 152 2 , , , cord-320015-lbr2q4qh 152 3 28 28 CD cord-320015-lbr2q4qh 152 4 ts ts JJ cord-320015-lbr2q4qh 152 5 mutants mutant NNS cord-320015-lbr2q4qh 152 6 were be VBD cord-320015-lbr2q4qh 152 7 organized organize VBN cord-320015-lbr2q4qh 152 8 into into IN cord-320015-lbr2q4qh 152 9 19 19 CD cord-320015-lbr2q4qh 152 10 complementation complementation NN cord-320015-lbr2q4qh 152 11 groups group NNS cord-320015-lbr2q4qh 152 12 comprising comprise VBG cord-320015-lbr2q4qh 152 13 three three CD cord-320015-lbr2q4qh 152 14 classes class NNS cord-320015-lbr2q4qh 152 15 [ [ -LRB- cord-320015-lbr2q4qh 152 16 47 47 CD cord-320015-lbr2q4qh 152 17 ] ] -RRB- cord-320015-lbr2q4qh 152 18 . . . cord-320015-lbr2q4qh 153 1 Class class NN cord-320015-lbr2q4qh 153 2 I -PRON- PRP cord-320015-lbr2q4qh 153 3 mutants mutant NNS cord-320015-lbr2q4qh 153 4 ( ( -LRB- cord-320015-lbr2q4qh 153 5 12 12 CD cord-320015-lbr2q4qh 153 6 complementation complementation NN cord-320015-lbr2q4qh 153 7 groups group NNS cord-320015-lbr2q4qh 153 8 ) ) -RRB- cord-320015-lbr2q4qh 153 9 appeared appear VBD cord-320015-lbr2q4qh 153 10 to to TO cord-320015-lbr2q4qh 153 11 contain contain VB cord-320015-lbr2q4qh 153 12 mutants mutant NNS cord-320015-lbr2q4qh 153 13 with with IN cord-320015-lbr2q4qh 153 14 defects defect NNS cord-320015-lbr2q4qh 153 15 in in IN cord-320015-lbr2q4qh 153 16 virus virus NN cord-320015-lbr2q4qh 153 17 assembly assembly NN cord-320015-lbr2q4qh 153 18 . . . cord-320015-lbr2q4qh 154 1 These these DT cord-320015-lbr2q4qh 154 2 mutants mutant NNS cord-320015-lbr2q4qh 154 3 synthesized synthesize VBD cord-320015-lbr2q4qh 154 4 early early RB cord-320015-lbr2q4qh 154 5 and and CC cord-320015-lbr2q4qh 154 6 late late JJ cord-320015-lbr2q4qh 154 7 viral viral JJ cord-320015-lbr2q4qh 154 8 proteins protein NNS cord-320015-lbr2q4qh 154 9 and and CC cord-320015-lbr2q4qh 154 10 viral viral JJ cord-320015-lbr2q4qh 154 11 DNA dna NN cord-320015-lbr2q4qh 154 12 , , , cord-320015-lbr2q4qh 154 13 but but CC cord-320015-lbr2q4qh 154 14 did do VBD cord-320015-lbr2q4qh 154 15 not not RB cord-320015-lbr2q4qh 154 16 generate generate VB cord-320015-lbr2q4qh 154 17 infectious infectious JJ cord-320015-lbr2q4qh 154 18 virions virion NNS cord-320015-lbr2q4qh 154 19 [ [ -LRB- cord-320015-lbr2q4qh 154 20 47 47 CD cord-320015-lbr2q4qh 154 21 ] ] -RRB- cord-320015-lbr2q4qh 154 22 . . . cord-320015-lbr2q4qh 155 1 Recently recently RB cord-320015-lbr2q4qh 155 2 , , , cord-320015-lbr2q4qh 155 3 TEM TEM NNP cord-320015-lbr2q4qh 155 4 analysis analysis NN cord-320015-lbr2q4qh 155 5 suggested suggest VBD cord-320015-lbr2q4qh 155 6 that that IN cord-320015-lbr2q4qh 155 7 this this DT cord-320015-lbr2q4qh 155 8 class class NN cord-320015-lbr2q4qh 155 9 could could MD cord-320015-lbr2q4qh 155 10 be be VB cord-320015-lbr2q4qh 155 11 further far RBR cord-320015-lbr2q4qh 155 12 sub sub NN cord-320015-lbr2q4qh 155 13 - - JJ cord-320015-lbr2q4qh 155 14 divided divided JJ cord-320015-lbr2q4qh 155 15 into into IN cord-320015-lbr2q4qh 155 16 at at RB cord-320015-lbr2q4qh 155 17 least least RBS cord-320015-lbr2q4qh 155 18 two two CD cord-320015-lbr2q4qh 155 19 subclasses subclass NNS cord-320015-lbr2q4qh 155 20 , , , cord-320015-lbr2q4qh 155 21 those those DT cord-320015-lbr2q4qh 155 22 that that WDT cord-320015-lbr2q4qh 155 23 failed fail VBD cord-320015-lbr2q4qh 155 24 to to TO cord-320015-lbr2q4qh 155 25 assemble assemble VB cord-320015-lbr2q4qh 155 26 icosahedral icosahedral JJ cord-320015-lbr2q4qh 155 27 particles particle NNS cord-320015-lbr2q4qh 155 28 and and CC cord-320015-lbr2q4qh 155 29 those those DT cord-320015-lbr2q4qh 155 30 that that WDT cord-320015-lbr2q4qh 155 31 formed form VBD cord-320015-lbr2q4qh 155 32 ostensibly ostensibly RB cord-320015-lbr2q4qh 155 33 normal normal JJ cord-320015-lbr2q4qh 155 34 , , , cord-320015-lbr2q4qh 155 35 but but CC cord-320015-lbr2q4qh 155 36 non non JJ cord-320015-lbr2q4qh 155 37 - - JJ cord-320015-lbr2q4qh 155 38 infectious infectious JJ cord-320015-lbr2q4qh 155 39 , , , cord-320015-lbr2q4qh 155 40 icosahedral icosahedral JJ cord-320015-lbr2q4qh 155 41 particles particle NNS cord-320015-lbr2q4qh 155 42 at at IN cord-320015-lbr2q4qh 155 43 the the DT cord-320015-lbr2q4qh 155 44 non non JJ cord-320015-lbr2q4qh 155 45 - - JJ cord-320015-lbr2q4qh 155 46 permissive permissive JJ cord-320015-lbr2q4qh 155 47 temperature temperature NN cord-320015-lbr2q4qh 155 48 [ [ -LRB- cord-320015-lbr2q4qh 155 49 115 115 CD cord-320015-lbr2q4qh 155 50 ] ] -RRB- cord-320015-lbr2q4qh 155 51 . . . cord-320015-lbr2q4qh 156 1 The the DT cord-320015-lbr2q4qh 156 2 remaining remain VBG cord-320015-lbr2q4qh 156 3 complementation complementation NN cord-320015-lbr2q4qh 156 4 groups group NNS cord-320015-lbr2q4qh 156 5 appeared appear VBD cord-320015-lbr2q4qh 156 6 to to TO cord-320015-lbr2q4qh 156 7 contain contain VB cord-320015-lbr2q4qh 156 8 defects defect NNS cord-320015-lbr2q4qh 156 9 in in IN cord-320015-lbr2q4qh 156 10 proteins protein NNS cord-320015-lbr2q4qh 156 11 associated associate VBN cord-320015-lbr2q4qh 156 12 with with IN cord-320015-lbr2q4qh 156 13 viral viral JJ cord-320015-lbr2q4qh 156 14 transcription transcription NN cord-320015-lbr2q4qh 156 15 ( ( -LRB- cord-320015-lbr2q4qh 156 16 Class Class NNP cord-320015-lbr2q4qh 156 17 II II NNP cord-320015-lbr2q4qh 156 18 ) ) -RRB- cord-320015-lbr2q4qh 156 19 and and CC cord-320015-lbr2q4qh 156 20 viral viral JJ cord-320015-lbr2q4qh 156 21 DNA dna NN cord-320015-lbr2q4qh 156 22 synthesis synthesis NN cord-320015-lbr2q4qh 156 23 ( ( -LRB- cord-320015-lbr2q4qh 156 24 Class Class NNP cord-320015-lbr2q4qh 156 25 III III NNP cord-320015-lbr2q4qh 156 26 ) ) -RRB- cord-320015-lbr2q4qh 156 27 . . . cord-320015-lbr2q4qh 157 1 Consistent consistent JJ cord-320015-lbr2q4qh 157 2 with with IN cord-320015-lbr2q4qh 157 3 an an DT cord-320015-lbr2q4qh 157 4 early early JJ cord-320015-lbr2q4qh 157 5 study study NN cord-320015-lbr2q4qh 157 6 suggesting suggest VBG cord-320015-lbr2q4qh 157 7 that that IN cord-320015-lbr2q4qh 157 8 only only JJ cord-320015-lbr2q4qh 157 9 early early JJ cord-320015-lbr2q4qh 157 10 viral viral JJ cord-320015-lbr2q4qh 157 11 proteins protein NNS cord-320015-lbr2q4qh 157 12 were be VBD cord-320015-lbr2q4qh 157 13 required require VBN cord-320015-lbr2q4qh 157 14 for for IN cord-320015-lbr2q4qh 157 15 assembly assembly NN cord-320015-lbr2q4qh 157 16 site site NN cord-320015-lbr2q4qh 157 17 formation formation NN cord-320015-lbr2q4qh 157 18 [ [ -LRB- cord-320015-lbr2q4qh 157 19 116 116 CD cord-320015-lbr2q4qh 157 20 ] ] -RRB- cord-320015-lbr2q4qh 157 21 , , , cord-320015-lbr2q4qh 157 22 TEM TEM NNP cord-320015-lbr2q4qh 157 23 analysis analysis NN cord-320015-lbr2q4qh 157 24 suggested suggest VBD cord-320015-lbr2q4qh 157 25 that that IN cord-320015-lbr2q4qh 157 26 full full JJ cord-320015-lbr2q4qh 157 27 viral viral JJ cord-320015-lbr2q4qh 157 28 DNA dna NN cord-320015-lbr2q4qh 157 29 synthesis synthesis NN cord-320015-lbr2q4qh 157 30 was be VBD cord-320015-lbr2q4qh 157 31 not not RB cord-320015-lbr2q4qh 157 32 required require VBN cord-320015-lbr2q4qh 157 33 for for IN cord-320015-lbr2q4qh 157 34 the the DT cord-320015-lbr2q4qh 157 35 formation formation NN cord-320015-lbr2q4qh 157 36 of of IN cord-320015-lbr2q4qh 157 37 viral viral JJ cord-320015-lbr2q4qh 157 38 assembly assembly NN cord-320015-lbr2q4qh 157 39 sites site NNS cord-320015-lbr2q4qh 157 40 as as IN cord-320015-lbr2q4qh 157 41 two two CD cord-320015-lbr2q4qh 157 42 ts t NNS cord-320015-lbr2q4qh 157 43 mutants mutant NNS cord-320015-lbr2q4qh 157 44 that that WDT cord-320015-lbr2q4qh 157 45 synthesized synthesize VBD cord-320015-lbr2q4qh 157 46 markedly markedly RB cord-320015-lbr2q4qh 157 47 reduced reduce VBN cord-320015-lbr2q4qh 157 48 levels level NNS cord-320015-lbr2q4qh 157 49 of of IN cord-320015-lbr2q4qh 157 50 viral viral JJ cord-320015-lbr2q4qh 157 51 DNA dna NN cord-320015-lbr2q4qh 157 52 were be VBD cord-320015-lbr2q4qh 157 53 able able JJ cord-320015-lbr2q4qh 157 54 to to TO cord-320015-lbr2q4qh 157 55 form form VB cord-320015-lbr2q4qh 157 56 large large JJ cord-320015-lbr2q4qh 157 57 VAS vas NN cord-320015-lbr2q4qh 157 58 , , , cord-320015-lbr2q4qh 157 59 that that WDT cord-320015-lbr2q4qh 157 60 were be VBD cord-320015-lbr2q4qh 157 61 devoid devoid JJ cord-320015-lbr2q4qh 157 62 of of IN cord-320015-lbr2q4qh 157 63 viral viral JJ cord-320015-lbr2q4qh 157 64 DNA dna NN cord-320015-lbr2q4qh 157 65 , , , cord-320015-lbr2q4qh 157 66 most most JJS cord-320015-lbr2q4qh 157 67 viral viral JJ cord-320015-lbr2q4qh 157 68 proteins protein NNS cord-320015-lbr2q4qh 157 69 , , , cord-320015-lbr2q4qh 157 70 and and CC cord-320015-lbr2q4qh 157 71 viral viral JJ cord-320015-lbr2q4qh 157 72 particles particle NNS cord-320015-lbr2q4qh 157 73 [ [ -LRB- cord-320015-lbr2q4qh 157 74 47 47 CD cord-320015-lbr2q4qh 157 75 , , , cord-320015-lbr2q4qh 157 76 115 115 CD cord-320015-lbr2q4qh 157 77 ] ] -RRB- cord-320015-lbr2q4qh 157 78 . . . cord-320015-lbr2q4qh 158 1 However however RB cord-320015-lbr2q4qh 158 2 , , , cord-320015-lbr2q4qh 158 3 although although IN cord-320015-lbr2q4qh 158 4 ts ts JJ cord-320015-lbr2q4qh 158 5 mutants mutant NNS cord-320015-lbr2q4qh 158 6 have have VBP cord-320015-lbr2q4qh 158 7 contributed contribute VBN cord-320015-lbr2q4qh 158 8 to to IN cord-320015-lbr2q4qh 158 9 our -PRON- PRP$ cord-320015-lbr2q4qh 158 10 understanding understanding NN cord-320015-lbr2q4qh 158 11 of of IN cord-320015-lbr2q4qh 158 12 FV3 FV3 NNP cord-320015-lbr2q4qh 158 13 replication replication NN cord-320015-lbr2q4qh 158 14 , , , cord-320015-lbr2q4qh 158 15 it -PRON- PRP cord-320015-lbr2q4qh 158 16 has have VBZ cord-320015-lbr2q4qh 158 17 not not RB cord-320015-lbr2q4qh 158 18 been be VBN cord-320015-lbr2q4qh 158 19 possible possible JJ cord-320015-lbr2q4qh 158 20 to to TO cord-320015-lbr2q4qh 158 21 associate associate VB cord-320015-lbr2q4qh 158 22 a a DT cord-320015-lbr2q4qh 158 23 given give VBN cord-320015-lbr2q4qh 158 24 mutant mutant JJ cord-320015-lbr2q4qh 158 25 phenotype phenotype NN cord-320015-lbr2q4qh 158 26 with with IN cord-320015-lbr2q4qh 158 27 a a DT cord-320015-lbr2q4qh 158 28 specific specific JJ cord-320015-lbr2q4qh 158 29 viral viral JJ cord-320015-lbr2q4qh 158 30 ORF orf NN cord-320015-lbr2q4qh 158 31 , , , cord-320015-lbr2q4qh 158 32 and and CC cord-320015-lbr2q4qh 158 33 thus thus RB cord-320015-lbr2q4qh 158 34 link link VBP cord-320015-lbr2q4qh 158 35 a a DT cord-320015-lbr2q4qh 158 36 specific specific JJ cord-320015-lbr2q4qh 158 37 viral viral JJ cord-320015-lbr2q4qh 158 38 protein protein NN cord-320015-lbr2q4qh 158 39 with with IN cord-320015-lbr2q4qh 158 40 its -PRON- PRP$ cord-320015-lbr2q4qh 158 41 function function NN cord-320015-lbr2q4qh 158 42 . . . cord-320015-lbr2q4qh 159 1 To to TO cord-320015-lbr2q4qh 159 2 address address VB cord-320015-lbr2q4qh 159 3 this this DT cord-320015-lbr2q4qh 159 4 issue issue NN cord-320015-lbr2q4qh 159 5 , , , cord-320015-lbr2q4qh 159 6 we -PRON- PRP cord-320015-lbr2q4qh 159 7 have have VBP cord-320015-lbr2q4qh 159 8 recently recently RB cord-320015-lbr2q4qh 159 9 developed develop VBN cord-320015-lbr2q4qh 159 10 approaches approach NNS cord-320015-lbr2q4qh 159 11 that that WDT cord-320015-lbr2q4qh 159 12 target target VBP cord-320015-lbr2q4qh 159 13 individual individual JJ cord-320015-lbr2q4qh 159 14 viral viral JJ cord-320015-lbr2q4qh 159 15 genes gene NNS cord-320015-lbr2q4qh 159 16 and and CC cord-320015-lbr2q4qh 159 17 have have VBP cord-320015-lbr2q4qh 159 18 used use VBN cord-320015-lbr2q4qh 159 19 them -PRON- PRP cord-320015-lbr2q4qh 159 20 to to TO cord-320015-lbr2q4qh 159 21 determine determine VB cord-320015-lbr2q4qh 159 22 viral viral JJ cord-320015-lbr2q4qh 159 23 gene gene NN cord-320015-lbr2q4qh 159 24 function function NN cord-320015-lbr2q4qh 159 25 by by IN cord-320015-lbr2q4qh 159 26 observing observe VBG cord-320015-lbr2q4qh 159 27 changes change NNS cord-320015-lbr2q4qh 159 28 in in IN cord-320015-lbr2q4qh 159 29 phenotype phenotype NN cord-320015-lbr2q4qh 159 30 . . . cord-320015-lbr2q4qh 160 1 To to TO cord-320015-lbr2q4qh 160 2 link link VB cord-320015-lbr2q4qh 160 3 specific specific JJ cord-320015-lbr2q4qh 160 4 viral viral JJ cord-320015-lbr2q4qh 160 5 proteins protein NNS cord-320015-lbr2q4qh 160 6 with with IN cord-320015-lbr2q4qh 160 7 their -PRON- PRP$ cord-320015-lbr2q4qh 160 8 functions function NNS cord-320015-lbr2q4qh 160 9 , , , cord-320015-lbr2q4qh 160 10 viral viral JJ cord-320015-lbr2q4qh 160 11 gene gene NN cord-320015-lbr2q4qh 160 12 expression expression NN cord-320015-lbr2q4qh 160 13 was be VBD cord-320015-lbr2q4qh 160 14 knocked knock VBN cord-320015-lbr2q4qh 160 15 down down RP cord-320015-lbr2q4qh 160 16 ( ( -LRB- cord-320015-lbr2q4qh 160 17 KD KD NNP cord-320015-lbr2q4qh 160 18 ) ) -RRB- cord-320015-lbr2q4qh 160 19 using use VBG cord-320015-lbr2q4qh 160 20 antisense antisense NN cord-320015-lbr2q4qh 160 21 morpholino morpholino NNP cord-320015-lbr2q4qh 160 22 oligonucleotides oligonucleotides NNP cord-320015-lbr2q4qh 160 23 ( ( -LRB- cord-320015-lbr2q4qh 160 24 asMOs asMOs NNP cord-320015-lbr2q4qh 160 25 ) ) -RRB- cord-320015-lbr2q4qh 160 26 and and CC cord-320015-lbr2q4qh 160 27 gene gene NN cord-320015-lbr2q4qh 160 28 function function NN cord-320015-lbr2q4qh 160 29 was be VBD cord-320015-lbr2q4qh 160 30 inferred infer VBN cord-320015-lbr2q4qh 160 31 by by IN cord-320015-lbr2q4qh 160 32 changes change NNS cord-320015-lbr2q4qh 160 33 in in IN cord-320015-lbr2q4qh 160 34 phenotype phenotype NN cord-320015-lbr2q4qh 160 35 . . . cord-320015-lbr2q4qh 161 1 AsMOs AsMOs NNP cord-320015-lbr2q4qh 161 2 are be VBP cord-320015-lbr2q4qh 161 3 DNA dna NN cord-320015-lbr2q4qh 161 4 - - HYPH cord-320015-lbr2q4qh 161 5 like like JJ cord-320015-lbr2q4qh 161 6 macromolecules macromolecule NNS cord-320015-lbr2q4qh 161 7 , , , cord-320015-lbr2q4qh 161 8 optimally optimally RB cord-320015-lbr2q4qh 161 9 25 25 CD cord-320015-lbr2q4qh 161 10 nucleotides nucleotide NNS cord-320015-lbr2q4qh 161 11 in in IN cord-320015-lbr2q4qh 161 12 length length NN cord-320015-lbr2q4qh 161 13 , , , cord-320015-lbr2q4qh 161 14 whose whose WP$ cord-320015-lbr2q4qh 161 15 backbone backbone NN cord-320015-lbr2q4qh 161 16 contains contain VBZ cord-320015-lbr2q4qh 161 17 morpholine morpholine NNP cord-320015-lbr2q4qh 161 18 rings ring NNS cord-320015-lbr2q4qh 161 19 instead instead RB cord-320015-lbr2q4qh 161 20 of of IN cord-320015-lbr2q4qh 161 21 deoxyribose deoxyribose JJ cord-320015-lbr2q4qh 161 22 rings ring NNS cord-320015-lbr2q4qh 161 23 and and CC cord-320015-lbr2q4qh 161 24 non non JJ cord-320015-lbr2q4qh 161 25 - - JJ cord-320015-lbr2q4qh 161 26 ionic ionic JJ cord-320015-lbr2q4qh 161 27 , , , cord-320015-lbr2q4qh 161 28 phosphorodiamidate phosphorodiamidate JJ cord-320015-lbr2q4qh 161 29 bonds bond NNS cord-320015-lbr2q4qh 161 30 in in IN cord-320015-lbr2q4qh 161 31 place place NN cord-320015-lbr2q4qh 161 32 of of IN cord-320015-lbr2q4qh 161 33 phosphodiester phosphodiester NN cord-320015-lbr2q4qh 161 34 links link NNS cord-320015-lbr2q4qh 161 35 [ [ -LRB- cord-320015-lbr2q4qh 161 36 117 117 CD cord-320015-lbr2q4qh 161 37 ] ] -RRB- cord-320015-lbr2q4qh 161 38 . . . cord-320015-lbr2q4qh 162 1 Moreover moreover RB cord-320015-lbr2q4qh 162 2 , , , cord-320015-lbr2q4qh 162 3 because because IN cord-320015-lbr2q4qh 162 4 phosphorodiamidate phosphorodiamidate JJ cord-320015-lbr2q4qh 162 5 bonds bond NNS cord-320015-lbr2q4qh 162 6 are be VBP cord-320015-lbr2q4qh 162 7 resistant resistant JJ cord-320015-lbr2q4qh 162 8 to to IN cord-320015-lbr2q4qh 162 9 degradation degradation NN cord-320015-lbr2q4qh 162 10 by by IN cord-320015-lbr2q4qh 162 11 cellular cellular JJ cord-320015-lbr2q4qh 162 12 nucleases nuclease NNS cord-320015-lbr2q4qh 162 13 , , , cord-320015-lbr2q4qh 162 14 asMOs asMOs NNP cord-320015-lbr2q4qh 162 15 are be VBP cord-320015-lbr2q4qh 162 16 remarkably remarkably RB cord-320015-lbr2q4qh 162 17 stable stable JJ cord-320015-lbr2q4qh 162 18 in in IN cord-320015-lbr2q4qh 162 19 cell cell NN cord-320015-lbr2q4qh 162 20 culture culture NN cord-320015-lbr2q4qh 162 21 [ [ -LRB- cord-320015-lbr2q4qh 162 22 117 117 CD cord-320015-lbr2q4qh 162 23 ] ] -RRB- cord-320015-lbr2q4qh 162 24 . . . cord-320015-lbr2q4qh 163 1 Furthermore furthermore RB cord-320015-lbr2q4qh 163 2 , , , cord-320015-lbr2q4qh 163 3 in in IN cord-320015-lbr2q4qh 163 4 contrast contrast NN cord-320015-lbr2q4qh 163 5 to to IN cord-320015-lbr2q4qh 163 6 siRNAs siRNAs NNP cord-320015-lbr2q4qh 163 7 that that WDT cord-320015-lbr2q4qh 163 8 sometimes sometimes RB cord-320015-lbr2q4qh 163 9 trigger trigger VBP cord-320015-lbr2q4qh 163 10 off off RP cord-320015-lbr2q4qh 163 11 - - HYPH cord-320015-lbr2q4qh 163 12 targeting target VBG cord-320015-lbr2q4qh 163 13 effects effect NNS cord-320015-lbr2q4qh 163 14 by by IN cord-320015-lbr2q4qh 163 15 activating activate VBG cord-320015-lbr2q4qh 163 16 pattern pattern NN cord-320015-lbr2q4qh 163 17 recognition recognition NN cord-320015-lbr2q4qh 163 18 receptors receptor NNS cord-320015-lbr2q4qh 163 19 such such JJ cord-320015-lbr2q4qh 163 20 as as IN cord-320015-lbr2q4qh 163 21 toll toll NN cord-320015-lbr2q4qh 163 22 - - HYPH cord-320015-lbr2q4qh 163 23 like like JJ cord-320015-lbr2q4qh 163 24 receptor receptor NN cord-320015-lbr2q4qh 163 25 3 3 CD cord-320015-lbr2q4qh 163 26 ( ( -LRB- cord-320015-lbr2q4qh 163 27 TLR-3 TLR-3 NNP cord-320015-lbr2q4qh 163 28 ) ) -RRB- cord-320015-lbr2q4qh 163 29 , , , cord-320015-lbr2q4qh 163 30 asMOs asMOs NNP cord-320015-lbr2q4qh 163 31 are be VBP cord-320015-lbr2q4qh 163 32 not not RB cord-320015-lbr2q4qh 163 33 recognized recognize VBN cord-320015-lbr2q4qh 163 34 by by IN cord-320015-lbr2q4qh 163 35 cellular cellular JJ cord-320015-lbr2q4qh 163 36 DNA dna NN cord-320015-lbr2q4qh 163 37 receptors receptor NNS cord-320015-lbr2q4qh 163 38 such such JJ cord-320015-lbr2q4qh 163 39 as as IN cord-320015-lbr2q4qh 163 40 TLR-9 TLR-9 NNP cord-320015-lbr2q4qh 163 41 or or CC cord-320015-lbr2q4qh 163 42 AIM-2 AIM-2 NNP cord-320015-lbr2q4qh 163 43 and and CC cord-320015-lbr2q4qh 163 44 thus thus RB cord-320015-lbr2q4qh 163 45 do do VBP cord-320015-lbr2q4qh 163 46 not not RB cord-320015-lbr2q4qh 163 47 induce induce VB cord-320015-lbr2q4qh 163 48 innate innate JJ cord-320015-lbr2q4qh 163 49 immune immune JJ cord-320015-lbr2q4qh 163 50 responses response NNS cord-320015-lbr2q4qh 163 51 . . . cord-320015-lbr2q4qh 164 1 asMOs asMOs NNP cord-320015-lbr2q4qh 164 2 bind bind VB cord-320015-lbr2q4qh 164 3 complimentary complimentary JJ cord-320015-lbr2q4qh 164 4 sequences sequence NNS cord-320015-lbr2q4qh 164 5 within within IN cord-320015-lbr2q4qh 164 6 the the DT cord-320015-lbr2q4qh 164 7 5 5 CD cord-320015-lbr2q4qh 164 8 ' ' `` cord-320015-lbr2q4qh 164 9 non non JJ cord-320015-lbr2q4qh 164 10 - - JJ cord-320015-lbr2q4qh 164 11 translated translate VBN cord-320015-lbr2q4qh 164 12 region region NN cord-320015-lbr2q4qh 164 13 , , , cord-320015-lbr2q4qh 164 14 or or CC cord-320015-lbr2q4qh 164 15 the the DT cord-320015-lbr2q4qh 164 16 region region NN cord-320015-lbr2q4qh 164 17 immediately immediately RB cord-320015-lbr2q4qh 164 18 surrounding surround VBG cord-320015-lbr2q4qh 164 19 the the DT cord-320015-lbr2q4qh 164 20 AUG AUG NNP cord-320015-lbr2q4qh 164 21 initiation initiation NN cord-320015-lbr2q4qh 164 22 codon codon NN cord-320015-lbr2q4qh 164 23 , , , cord-320015-lbr2q4qh 164 24 of of IN cord-320015-lbr2q4qh 164 25 the the DT cord-320015-lbr2q4qh 164 26 targeted target VBN cord-320015-lbr2q4qh 164 27 mRNA mrna NN cord-320015-lbr2q4qh 164 28 and and CC cord-320015-lbr2q4qh 164 29 are be VBP cord-320015-lbr2q4qh 164 30 thought think VBN cord-320015-lbr2q4qh 164 31 to to TO cord-320015-lbr2q4qh 164 32 block block VB cord-320015-lbr2q4qh 164 33 scanning scanning NN cord-320015-lbr2q4qh 164 34 of of IN cord-320015-lbr2q4qh 164 35 the the DT cord-320015-lbr2q4qh 164 36 40S 40s CD cord-320015-lbr2q4qh 164 37 ribosome ribosome NN cord-320015-lbr2q4qh 164 38 by by IN cord-320015-lbr2q4qh 164 39 steric steric JJ cord-320015-lbr2q4qh 164 40 hindrance hindrance NN cord-320015-lbr2q4qh 164 41 . . . cord-320015-lbr2q4qh 165 1 In in IN cord-320015-lbr2q4qh 165 2 addition addition NN cord-320015-lbr2q4qh 165 3 , , , cord-320015-lbr2q4qh 165 4 asMOs asMOs NNP cord-320015-lbr2q4qh 165 5 also also RB cord-320015-lbr2q4qh 165 6 block block VBP cord-320015-lbr2q4qh 165 7 gene gene NN cord-320015-lbr2q4qh 165 8 expression expression NN cord-320015-lbr2q4qh 165 9 by by IN cord-320015-lbr2q4qh 165 10 inhibiting inhibit VBG cord-320015-lbr2q4qh 165 11 splicing splicing NN cord-320015-lbr2q4qh 165 12 or or CC cord-320015-lbr2q4qh 165 13 preventing prevent VBG cord-320015-lbr2q4qh 165 14 interaction interaction NN cord-320015-lbr2q4qh 165 15 of of IN cord-320015-lbr2q4qh 165 16 regulatory regulatory JJ cord-320015-lbr2q4qh 165 17 proteins protein NNS cord-320015-lbr2q4qh 165 18 with with IN cord-320015-lbr2q4qh 165 19 their -PRON- PRP$ cord-320015-lbr2q4qh 165 20 specific specific JJ cord-320015-lbr2q4qh 165 21 target target NN cord-320015-lbr2q4qh 165 22 sequence sequence NN cord-320015-lbr2q4qh 165 23 [ [ -LRB- cord-320015-lbr2q4qh 165 24 118 118 CD cord-320015-lbr2q4qh 165 25 ] ] -RRB- cord-320015-lbr2q4qh 165 26 . . . cord-320015-lbr2q4qh 166 1 Collectively collectively RB cord-320015-lbr2q4qh 166 2 asMOs asMOs NNP cord-320015-lbr2q4qh 166 3 have have VBP cord-320015-lbr2q4qh 166 4 been be VBN cord-320015-lbr2q4qh 166 5 used use VBN cord-320015-lbr2q4qh 166 6 to to TO cord-320015-lbr2q4qh 166 7 block block VB cord-320015-lbr2q4qh 166 8 cellular cellular JJ cord-320015-lbr2q4qh 166 9 and and CC cord-320015-lbr2q4qh 166 10 viral viral JJ cord-320015-lbr2q4qh 166 11 gene gene NN cord-320015-lbr2q4qh 166 12 expression expression NN cord-320015-lbr2q4qh 166 13 both both CC cord-320015-lbr2q4qh 166 14 in in IN cord-320015-lbr2q4qh 166 15 vitro vitro FW cord-320015-lbr2q4qh 166 16 and and CC cord-320015-lbr2q4qh 166 17 in in IN cord-320015-lbr2q4qh 166 18 vivo vivo NNP cord-320015-lbr2q4qh 166 19 [ [ -LRB- cord-320015-lbr2q4qh 166 20 119 119 CD cord-320015-lbr2q4qh 166 21 ] ] -RRB- cord-320015-lbr2q4qh 167 1 [ [ -LRB- cord-320015-lbr2q4qh 167 2 120 120 CD cord-320015-lbr2q4qh 167 3 ] ] -RRB- cord-320015-lbr2q4qh 168 1 [ [ -LRB- cord-320015-lbr2q4qh 168 2 121 121 CD cord-320015-lbr2q4qh 168 3 ] ] -RRB- cord-320015-lbr2q4qh 168 4 . . . cord-320015-lbr2q4qh 169 1 AsMOs AsMOs NNP cord-320015-lbr2q4qh 169 2 have have VBP cord-320015-lbr2q4qh 169 3 been be VBN cord-320015-lbr2q4qh 169 4 used use VBN cord-320015-lbr2q4qh 169 5 to to TO cord-320015-lbr2q4qh 169 6 ascertain ascertain VB cord-320015-lbr2q4qh 169 7 the the DT cord-320015-lbr2q4qh 169 8 function function NN cord-320015-lbr2q4qh 169 9 of of IN cord-320015-lbr2q4qh 169 10 several several JJ cord-320015-lbr2q4qh 169 11 FV3 FV3 NNP cord-320015-lbr2q4qh 169 12 genes gene NNS cord-320015-lbr2q4qh 169 13 including include VBG cord-320015-lbr2q4qh 169 14 those those DT cord-320015-lbr2q4qh 169 15 encoding encode VBG cord-320015-lbr2q4qh 169 16 the the DT cord-320015-lbr2q4qh 169 17 MCP MCP NNP cord-320015-lbr2q4qh 169 18 , , , cord-320015-lbr2q4qh 169 19 vPol vPol NNP cord-320015-lbr2q4qh 169 20 - - HYPH cord-320015-lbr2q4qh 169 21 IIα IIα NNP cord-320015-lbr2q4qh 169 22 , , , cord-320015-lbr2q4qh 169 23 an an DT cord-320015-lbr2q4qh 169 24 18 18 CD cord-320015-lbr2q4qh 169 25 kDa kDa NNPS cord-320015-lbr2q4qh 169 26 immediate immediate JJ cord-320015-lbr2q4qh 169 27 - - HYPH cord-320015-lbr2q4qh 169 28 early early JJ cord-320015-lbr2q4qh 169 29 viral viral JJ cord-320015-lbr2q4qh 169 30 gene gene NN cord-320015-lbr2q4qh 169 31 product product NN cord-320015-lbr2q4qh 169 32 ( ( -LRB- cord-320015-lbr2q4qh 169 33 18 18 CD cord-320015-lbr2q4qh 169 34 K K NNP cord-320015-lbr2q4qh 169 35 ) ) -RRB- cord-320015-lbr2q4qh 169 36 , , , cord-320015-lbr2q4qh 169 37 a a DT cord-320015-lbr2q4qh 169 38 putative putative JJ cord-320015-lbr2q4qh 169 39 myristoylated myristoylate VBN cord-320015-lbr2q4qh 169 40 membrane membrane NN cord-320015-lbr2q4qh 169 41 protein protein NN cord-320015-lbr2q4qh 169 42 ( ( -LRB- cord-320015-lbr2q4qh 169 43 53R 53r CD cord-320015-lbr2q4qh 169 44 ) ) -RRB- cord-320015-lbr2q4qh 169 45 , , , cord-320015-lbr2q4qh 169 46 and and CC cord-320015-lbr2q4qh 169 47 two two CD cord-320015-lbr2q4qh 169 48 IE IE NNP cord-320015-lbr2q4qh 169 49 proteins protein NNS cord-320015-lbr2q4qh 169 50 of of IN cord-320015-lbr2q4qh 169 51 unknown unknown JJ cord-320015-lbr2q4qh 169 52 function function NN cord-320015-lbr2q4qh 169 53 : : : cord-320015-lbr2q4qh 169 54 a a DT cord-320015-lbr2q4qh 169 55 70 70 CD cord-320015-lbr2q4qh 169 56 kDa kDa NNP cord-320015-lbr2q4qh 169 57 protein protein NN cord-320015-lbr2q4qh 169 58 designated designate VBN cord-320015-lbr2q4qh 169 59 32R 32r CD cord-320015-lbr2q4qh 169 60 , , , cord-320015-lbr2q4qh 169 61 and and CC cord-320015-lbr2q4qh 170 1 46 46 CD cord-320015-lbr2q4qh 170 2 kDa kDa NNP cord-320015-lbr2q4qh 170 3 protein protein NN cord-320015-lbr2q4qh 170 4 termed term VBD cord-320015-lbr2q4qh 170 5 46 46 CD cord-320015-lbr2q4qh 170 6 K K NNP cord-320015-lbr2q4qh 170 7 [ [ -LRB- cord-320015-lbr2q4qh 170 8 20 20 CD cord-320015-lbr2q4qh 170 9 , , , cord-320015-lbr2q4qh 170 10 52 52 CD cord-320015-lbr2q4qh 170 11 , , , cord-320015-lbr2q4qh 170 12 122 122 CD cord-320015-lbr2q4qh 170 13 , , , cord-320015-lbr2q4qh 170 14 123 123 CD cord-320015-lbr2q4qh 170 15 ] ] -RRB- cord-320015-lbr2q4qh 170 16 . . . cord-320015-lbr2q4qh 171 1 In in IN cord-320015-lbr2q4qh 171 2 an an DT cord-320015-lbr2q4qh 171 3 initial initial JJ cord-320015-lbr2q4qh 171 4 proof proof NN cord-320015-lbr2q4qh 171 5 - - HYPH cord-320015-lbr2q4qh 171 6 of of IN cord-320015-lbr2q4qh 171 7 - - HYPH cord-320015-lbr2q4qh 171 8 concept concept NN cord-320015-lbr2q4qh 171 9 study study NN cord-320015-lbr2q4qh 171 10 , , , cord-320015-lbr2q4qh 171 11 Sample Sample NNP cord-320015-lbr2q4qh 171 12 et et FW cord-320015-lbr2q4qh 171 13 al al NNP cord-320015-lbr2q4qh 171 14 . . . cord-320015-lbr2q4qh 172 1 [ [ -LRB- cord-320015-lbr2q4qh 172 2 52 52 CD cord-320015-lbr2q4qh 172 3 ] ] -RRB- cord-320015-lbr2q4qh 172 4 showed show VBD cord-320015-lbr2q4qh 172 5 that that IN cord-320015-lbr2q4qh 172 6 asMO asmo NN cord-320015-lbr2q4qh 172 7 treatment treatment NN cord-320015-lbr2q4qh 172 8 knocked knock VBD cord-320015-lbr2q4qh 172 9 down down RP cord-320015-lbr2q4qh 172 10 MCP MCP NNP cord-320015-lbr2q4qh 172 11 expression expression NN cord-320015-lbr2q4qh 172 12 by by IN cord-320015-lbr2q4qh 172 13 greater great JJR cord-320015-lbr2q4qh 172 14 than than IN cord-320015-lbr2q4qh 172 15 80 80 CD cord-320015-lbr2q4qh 172 16 % % NN cord-320015-lbr2q4qh 172 17 and and CC cord-320015-lbr2q4qh 172 18 resulted result VBD cord-320015-lbr2q4qh 172 19 in in IN cord-320015-lbr2q4qh 172 20 a a DT cord-320015-lbr2q4qh 172 21 corresponding corresponding JJ cord-320015-lbr2q4qh 172 22 drop drop NN cord-320015-lbr2q4qh 172 23 in in IN cord-320015-lbr2q4qh 172 24 the the DT cord-320015-lbr2q4qh 172 25 production production NN cord-320015-lbr2q4qh 172 26 of of IN cord-320015-lbr2q4qh 172 27 infectious infectious JJ cord-320015-lbr2q4qh 172 28 virions virion NNS cord-320015-lbr2q4qh 172 29 without without IN cord-320015-lbr2q4qh 172 30 any any DT cord-320015-lbr2q4qh 172 31 collateral collateral JJ cord-320015-lbr2q4qh 172 32 adverse adverse JJ cord-320015-lbr2q4qh 172 33 effects effect NNS cord-320015-lbr2q4qh 172 34 on on IN cord-320015-lbr2q4qh 172 35 the the DT cord-320015-lbr2q4qh 172 36 expression expression NN cord-320015-lbr2q4qh 172 37 of of IN cord-320015-lbr2q4qh 172 38 other other JJ cord-320015-lbr2q4qh 172 39 viral viral JJ cord-320015-lbr2q4qh 172 40 gene gene NN cord-320015-lbr2q4qh 172 41 products product NNS cord-320015-lbr2q4qh 172 42 . . . cord-320015-lbr2q4qh 173 1 Moreover moreover RB cord-320015-lbr2q4qh 173 2 , , , cord-320015-lbr2q4qh 173 3 MCP MCP NNP cord-320015-lbr2q4qh 173 4 knock knock VBP cord-320015-lbr2q4qh 173 5 down down RP cord-320015-lbr2q4qh 173 6 was be VBD cord-320015-lbr2q4qh 173 7 accompanied accompany VBN cord-320015-lbr2q4qh 173 8 by by IN cord-320015-lbr2q4qh 173 9 the the DT cord-320015-lbr2q4qh 173 10 appearance appearance NN cord-320015-lbr2q4qh 173 11 of of IN cord-320015-lbr2q4qh 173 12 atypical atypical JJ cord-320015-lbr2q4qh 173 13 elements element NNS cord-320015-lbr2q4qh 173 14 within within IN cord-320015-lbr2q4qh 173 15 VAS VAS NNP cord-320015-lbr2q4qh 173 16 suggesting suggest VBG cord-320015-lbr2q4qh 173 17 that that IN cord-320015-lbr2q4qh 173 18 in in IN cord-320015-lbr2q4qh 173 19 the the DT cord-320015-lbr2q4qh 173 20 absence absence NN cord-320015-lbr2q4qh 173 21 of of IN cord-320015-lbr2q4qh 173 22 full full JJ cord-320015-lbr2q4qh 173 23 MCP MCP NNP cord-320015-lbr2q4qh 173 24 expression expression NN cord-320015-lbr2q4qh 173 25 aberrant aberrant JJ cord-320015-lbr2q4qh 173 26 viral viral JJ cord-320015-lbr2q4qh 173 27 structures structure NNS cord-320015-lbr2q4qh 173 28 , , , cord-320015-lbr2q4qh 173 29 perhaps perhaps RB cord-320015-lbr2q4qh 173 30 representing represent VBG cord-320015-lbr2q4qh 173 31 altered alter VBN cord-320015-lbr2q4qh 173 32 products product NNS cord-320015-lbr2q4qh 173 33 of of IN cord-320015-lbr2q4qh 173 34 virion virion NN cord-320015-lbr2q4qh 173 35 assembly assembly NN cord-320015-lbr2q4qh 173 36 , , , cord-320015-lbr2q4qh 173 37 were be VBD cord-320015-lbr2q4qh 173 38 generated generate VBN cord-320015-lbr2q4qh 173 39 . . . cord-320015-lbr2q4qh 174 1 In in IN cord-320015-lbr2q4qh 174 2 the the DT cord-320015-lbr2q4qh 174 3 same same JJ cord-320015-lbr2q4qh 174 4 study study NN cord-320015-lbr2q4qh 174 5 , , , cord-320015-lbr2q4qh 174 6 KD KD NNP cord-320015-lbr2q4qh 174 7 of of IN cord-320015-lbr2q4qh 174 8 vPOL vpol JJ cord-320015-lbr2q4qh 174 9 - - JJ cord-320015-lbr2q4qh 174 10 IIα iiα NN cord-320015-lbr2q4qh 174 11 was be VBD cord-320015-lbr2q4qh 174 12 shown show VBN cord-320015-lbr2q4qh 174 13 to to TO cord-320015-lbr2q4qh 174 14 result result VB cord-320015-lbr2q4qh 174 15 in in IN cord-320015-lbr2q4qh 174 16 a a DT cord-320015-lbr2q4qh 174 17 marked marked JJ cord-320015-lbr2q4qh 174 18 reduction reduction NN cord-320015-lbr2q4qh 174 19 in in IN cord-320015-lbr2q4qh 174 20 the the DT cord-320015-lbr2q4qh 174 21 synthesis synthesis NN cord-320015-lbr2q4qh 174 22 of of IN cord-320015-lbr2q4qh 174 23 late late JJ cord-320015-lbr2q4qh 174 24 viral viral JJ cord-320015-lbr2q4qh 174 25 proteins protein NNS cord-320015-lbr2q4qh 174 26 . . . cord-320015-lbr2q4qh 175 1 This this DT cord-320015-lbr2q4qh 175 2 result result NN cord-320015-lbr2q4qh 175 3 provided provide VBD cord-320015-lbr2q4qh 175 4 the the DT cord-320015-lbr2q4qh 175 5 first first JJ cord-320015-lbr2q4qh 175 6 formal formal JJ cord-320015-lbr2q4qh 175 7 evidence evidence NN cord-320015-lbr2q4qh 175 8 that that IN cord-320015-lbr2q4qh 175 9 viral viral JJ cord-320015-lbr2q4qh 175 10 homologs homolog NNS cord-320015-lbr2q4qh 175 11 of of IN cord-320015-lbr2q4qh 175 12 host host NN cord-320015-lbr2q4qh 175 13 RNA RNA NNP cord-320015-lbr2q4qh 175 14 polymerase polymerase NN cord-320015-lbr2q4qh 175 15 II II NNP cord-320015-lbr2q4qh 175 16 played play VBD cord-320015-lbr2q4qh 175 17 a a DT cord-320015-lbr2q4qh 175 18 role role NN cord-320015-lbr2q4qh 175 19 in in IN cord-320015-lbr2q4qh 175 20 the the DT cord-320015-lbr2q4qh 175 21 synthesis synthesis NN cord-320015-lbr2q4qh 175 22 of of IN cord-320015-lbr2q4qh 175 23 late late JJ cord-320015-lbr2q4qh 175 24 viral viral JJ cord-320015-lbr2q4qh 175 25 transcripts transcript NNS cord-320015-lbr2q4qh 175 26 . . . cord-320015-lbr2q4qh 176 1 Lastly lastly RB cord-320015-lbr2q4qh 176 2 , , , cord-320015-lbr2q4qh 176 3 Sample sample VB cord-320015-lbr2q4qh 176 4 and and CC cord-320015-lbr2q4qh 176 5 co co NNS cord-320015-lbr2q4qh 176 6 - - NNS cord-320015-lbr2q4qh 176 7 workers worker NNS cord-320015-lbr2q4qh 176 8 observed observe VBD cord-320015-lbr2q4qh 176 9 that that IN cord-320015-lbr2q4qh 176 10 KD KD NNP cord-320015-lbr2q4qh 176 11 of of IN cord-320015-lbr2q4qh 176 12 the the DT cord-320015-lbr2q4qh 176 13 18 18 CD cord-320015-lbr2q4qh 176 14 K K NNP cord-320015-lbr2q4qh 176 15 IE IE NNP cord-320015-lbr2q4qh 176 16 protein protein NN cord-320015-lbr2q4qh 176 17 had have VBD cord-320015-lbr2q4qh 176 18 no no DT cord-320015-lbr2q4qh 176 19 observable observable JJ cord-320015-lbr2q4qh 176 20 effect effect NN cord-320015-lbr2q4qh 176 21 on on IN cord-320015-lbr2q4qh 176 22 viral viral JJ cord-320015-lbr2q4qh 176 23 gene gene NN cord-320015-lbr2q4qh 176 24 expression expression NN cord-320015-lbr2q4qh 176 25 or or CC cord-320015-lbr2q4qh 176 26 replication replication NN cord-320015-lbr2q4qh 176 27 in in IN cord-320015-lbr2q4qh 176 28 vitro vitro FW cord-320015-lbr2q4qh 176 29 suggesting suggest VBG cord-320015-lbr2q4qh 176 30 that that IN cord-320015-lbr2q4qh 176 31 , , , cord-320015-lbr2q4qh 176 32 at at IN cord-320015-lbr2q4qh 176 33 least least JJS cord-320015-lbr2q4qh 176 34 in in IN cord-320015-lbr2q4qh 176 35 fathead fathead NN cord-320015-lbr2q4qh 176 36 minnow minnow NN cord-320015-lbr2q4qh 176 37 cells cell NNS cord-320015-lbr2q4qh 176 38 , , , cord-320015-lbr2q4qh 176 39 18 18 CD cord-320015-lbr2q4qh 176 40 K K NNP cord-320015-lbr2q4qh 176 41 was be VBD cord-320015-lbr2q4qh 176 42 not not RB cord-320015-lbr2q4qh 176 43 required require VBN cord-320015-lbr2q4qh 176 44 for for IN cord-320015-lbr2q4qh 176 45 the the DT cord-320015-lbr2q4qh 176 46 production production NN cord-320015-lbr2q4qh 176 47 of of IN cord-320015-lbr2q4qh 176 48 infectious infectious JJ cord-320015-lbr2q4qh 176 49 virions virion NNS cord-320015-lbr2q4qh 176 50 . . . cord-320015-lbr2q4qh 177 1 Subsequent subsequent JJ cord-320015-lbr2q4qh 177 2 KD KD NNP cord-320015-lbr2q4qh 177 3 studies study NNS cord-320015-lbr2q4qh 177 4 of of IN cord-320015-lbr2q4qh 177 5 53R 53r CD cord-320015-lbr2q4qh 177 6 , , , cord-320015-lbr2q4qh 177 7 a a DT cord-320015-lbr2q4qh 177 8 putative putative JJ cord-320015-lbr2q4qh 177 9 virus virus NN cord-320015-lbr2q4qh 177 10 - - HYPH cord-320015-lbr2q4qh 177 11 encoded encode VBN cord-320015-lbr2q4qh 177 12 myristoylated myristoylate VBN cord-320015-lbr2q4qh 177 13 membrane membrane NN cord-320015-lbr2q4qh 177 14 protein protein NN cord-320015-lbr2q4qh 177 15 , , , cord-320015-lbr2q4qh 177 16 32R 32r CD cord-320015-lbr2q4qh 177 17 , , , cord-320015-lbr2q4qh 177 18 and and CC cord-320015-lbr2q4qh 178 1 46 46 CD cord-320015-lbr2q4qh 178 2 K K NNP cord-320015-lbr2q4qh 178 3 confirmed confirm VBD cord-320015-lbr2q4qh 178 4 an an DT cord-320015-lbr2q4qh 178 5 essential essential JJ cord-320015-lbr2q4qh 178 6 role role NN cord-320015-lbr2q4qh 178 7 for for IN cord-320015-lbr2q4qh 178 8 each each DT cord-320015-lbr2q4qh 178 9 of of IN cord-320015-lbr2q4qh 178 10 these these DT cord-320015-lbr2q4qh 178 11 proteins protein NNS cord-320015-lbr2q4qh 178 12 in in IN cord-320015-lbr2q4qh 178 13 FV3 FV3 NNP cord-320015-lbr2q4qh 178 14 biogenesis biogenesis NN cord-320015-lbr2q4qh 178 15 [ [ -LRB- cord-320015-lbr2q4qh 178 16 20 20 CD cord-320015-lbr2q4qh 178 17 , , , cord-320015-lbr2q4qh 178 18 122 122 CD cord-320015-lbr2q4qh 178 19 ] ] -RRB- cord-320015-lbr2q4qh 178 20 . . . cord-320015-lbr2q4qh 179 1 KD KD NNP cord-320015-lbr2q4qh 179 2 of of IN cord-320015-lbr2q4qh 179 3 53R 53R NNP cord-320015-lbr2q4qh 179 4 resulted result VBD cord-320015-lbr2q4qh 179 5 in in IN cord-320015-lbr2q4qh 179 6 the the DT cord-320015-lbr2q4qh 179 7 appearance appearance NN cord-320015-lbr2q4qh 179 8 of of IN cord-320015-lbr2q4qh 179 9 putative putative JJ cord-320015-lbr2q4qh 179 10 non non JJ cord-320015-lbr2q4qh 179 11 - - JJ cord-320015-lbr2q4qh 179 12 encapsidated encapsidated JJ cord-320015-lbr2q4qh 179 13 viral viral JJ cord-320015-lbr2q4qh 179 14 DNA dna NN cord-320015-lbr2q4qh 179 15 cores core NNS cord-320015-lbr2q4qh 179 16 within within IN cord-320015-lbr2q4qh 179 17 VAS VAS NNP cord-320015-lbr2q4qh 179 18 and and CC cord-320015-lbr2q4qh 179 19 supported support VBD cord-320015-lbr2q4qh 179 20 the the DT cord-320015-lbr2q4qh 179 21 view view NN cord-320015-lbr2q4qh 179 22 that that WDT cord-320015-lbr2q4qh 179 23 53R 53r CD cord-320015-lbr2q4qh 179 24 plays play VBZ cord-320015-lbr2q4qh 179 25 a a DT cord-320015-lbr2q4qh 179 26 major major JJ cord-320015-lbr2q4qh 179 27 role role NN cord-320015-lbr2q4qh 179 28 in in IN cord-320015-lbr2q4qh 179 29 capsid capsid JJ cord-320015-lbr2q4qh 179 30 formation formation NN cord-320015-lbr2q4qh 179 31 . . . cord-320015-lbr2q4qh 180 1 KD KD NNP cord-320015-lbr2q4qh 180 2 of of IN cord-320015-lbr2q4qh 180 3 32R 32r CD cord-320015-lbr2q4qh 180 4 and and CC cord-320015-lbr2q4qh 180 5 46 46 CD cord-320015-lbr2q4qh 180 6 K k NN cord-320015-lbr2q4qh 180 7 , , , cord-320015-lbr2q4qh 180 8 two two CD cord-320015-lbr2q4qh 180 9 IE IE NNP cord-320015-lbr2q4qh 180 10 proteins protein NNS cord-320015-lbr2q4qh 180 11 , , , cord-320015-lbr2q4qh 180 12 did do VBD cord-320015-lbr2q4qh 180 13 not not RB cord-320015-lbr2q4qh 180 14 affect affect VB cord-320015-lbr2q4qh 180 15 the the DT cord-320015-lbr2q4qh 180 16 synthesis synthesis NN cord-320015-lbr2q4qh 180 17 of of IN cord-320015-lbr2q4qh 180 18 other other JJ cord-320015-lbr2q4qh 180 19 viral viral JJ cord-320015-lbr2q4qh 180 20 proteins protein NNS cord-320015-lbr2q4qh 180 21 , , , cord-320015-lbr2q4qh 180 22 but but CC cord-320015-lbr2q4qh 180 23 resulted result VBD cord-320015-lbr2q4qh 180 24 in in IN cord-320015-lbr2q4qh 180 25 a a DT cord-320015-lbr2q4qh 180 26 > > XX cord-320015-lbr2q4qh 180 27 80 80 CD cord-320015-lbr2q4qh 180 28 % % NN cord-320015-lbr2q4qh 180 29 reduction reduction NN cord-320015-lbr2q4qh 180 30 in in IN cord-320015-lbr2q4qh 180 31 virion virion NN cord-320015-lbr2q4qh 180 32 formation formation NN cord-320015-lbr2q4qh 180 33 . . . cord-320015-lbr2q4qh 181 1 TEM TEM NNP cord-320015-lbr2q4qh 181 2 study study NN cord-320015-lbr2q4qh 181 3 showed show VBD cord-320015-lbr2q4qh 181 4 that that IN cord-320015-lbr2q4qh 181 5 cells cell NNS cord-320015-lbr2q4qh 181 6 treated treat VBN cord-320015-lbr2q4qh 181 7 with with IN cord-320015-lbr2q4qh 181 8 asMOs asmos NN cord-320015-lbr2q4qh 181 9 targeting target VBG cord-320015-lbr2q4qh 181 10 either either CC cord-320015-lbr2q4qh 181 11 32R 32r CD cord-320015-lbr2q4qh 181 12 or or CC cord-320015-lbr2q4qh 181 13 46 46 CD cord-320015-lbr2q4qh 181 14 K k NN cord-320015-lbr2q4qh 181 15 formed form VBD cord-320015-lbr2q4qh 181 16 viral viral JJ cord-320015-lbr2q4qh 181 17 assembly assembly NN cord-320015-lbr2q4qh 181 18 sites site NNS cord-320015-lbr2q4qh 181 19 , , , cord-320015-lbr2q4qh 181 20 but but CC cord-320015-lbr2q4qh 181 21 failed fail VBD cord-320015-lbr2q4qh 181 22 to to TO cord-320015-lbr2q4qh 181 23 form form VB cord-320015-lbr2q4qh 181 24 virions virion NNS cord-320015-lbr2q4qh 181 25 or or CC cord-320015-lbr2q4qh 181 26 recognizable recognizable JJ cord-320015-lbr2q4qh 181 27 assembly assembly NN cord-320015-lbr2q4qh 181 28 intermediates intermediate NNS cord-320015-lbr2q4qh 181 29 . . . cord-320015-lbr2q4qh 182 1 Studies study NNS cord-320015-lbr2q4qh 182 2 targeting target VBG cord-320015-lbr2q4qh 182 3 the the DT cord-320015-lbr2q4qh 182 4 virus virus NN cord-320015-lbr2q4qh 182 5 - - HYPH cord-320015-lbr2q4qh 182 6 encoded encode VBN cord-320015-lbr2q4qh 182 7 RNAse RNAse NNP cord-320015-lbr2q4qh 182 8 III III NNP cord-320015-lbr2q4qh 182 9 - - HYPH cord-320015-lbr2q4qh 182 10 like like JJ cord-320015-lbr2q4qh 182 11 protein protein NN cord-320015-lbr2q4qh 182 12 ( ( -LRB- cord-320015-lbr2q4qh 182 13 ORF ORF NNP cord-320015-lbr2q4qh 182 14 80L 80L NNP cord-320015-lbr2q4qh 182 15 ) ) -RRB- cord-320015-lbr2q4qh 182 16 , , , cord-320015-lbr2q4qh 182 17 a a DT cord-320015-lbr2q4qh 182 18 putative putative JJ cord-320015-lbr2q4qh 182 19 NTPase NTPase NNP cord-320015-lbr2q4qh 182 20 ( ( -LRB- cord-320015-lbr2q4qh 182 21 ORF ORF NNP cord-320015-lbr2q4qh 182 22 9L 9l CD cord-320015-lbr2q4qh 182 23 ) ) -RRB- cord-320015-lbr2q4qh 182 24 , , , cord-320015-lbr2q4qh 182 25 the the DT cord-320015-lbr2q4qh 182 26 largest large JJS cord-320015-lbr2q4qh 182 27 subunit subunit NN cord-320015-lbr2q4qh 182 28 of of IN cord-320015-lbr2q4qh 182 29 ribonucleotide ribonucleotide NN cord-320015-lbr2q4qh 182 30 reductase reductase NN cord-320015-lbr2q4qh 182 31 ( ( -LRB- cord-320015-lbr2q4qh 182 32 ORF ORF NNP cord-320015-lbr2q4qh 182 33 38R 38R NNP cord-320015-lbr2q4qh 182 34 ) ) -RRB- cord-320015-lbr2q4qh 182 35 , , , cord-320015-lbr2q4qh 182 36 a a DT cord-320015-lbr2q4qh 182 37 putative putative JJ cord-320015-lbr2q4qh 182 38 viral viral JJ cord-320015-lbr2q4qh 182 39 membrane membrane NN cord-320015-lbr2q4qh 182 40 protein protein NN cord-320015-lbr2q4qh 182 41 ( ( -LRB- cord-320015-lbr2q4qh 182 42 ORF2L ORF2L NNP cord-320015-lbr2q4qh 182 43 ) ) -RRB- cord-320015-lbr2q4qh 182 44 , , , cord-320015-lbr2q4qh 182 45 a a DT cord-320015-lbr2q4qh 182 46 DNA dna NN cord-320015-lbr2q4qh 182 47 packaging packaging NN cord-320015-lbr2q4qh 182 48 protein protein NN cord-320015-lbr2q4qh 182 49 ( ( -LRB- cord-320015-lbr2q4qh 182 50 ORF ORF NNP cord-320015-lbr2q4qh 182 51 1R 1R NNP cord-320015-lbr2q4qh 182 52 ) ) -RRB- cord-320015-lbr2q4qh 182 53 , , , cord-320015-lbr2q4qh 182 54 a a DT cord-320015-lbr2q4qh 182 55 putative putative JJ cord-320015-lbr2q4qh 182 56 serine serine NN cord-320015-lbr2q4qh 182 57 / / SYM cord-320015-lbr2q4qh 182 58 threonine threonine NN cord-320015-lbr2q4qh 182 59 kinase kinase NN cord-320015-lbr2q4qh 182 60 ( ( -LRB- cord-320015-lbr2q4qh 182 61 ORF ORF NNP cord-320015-lbr2q4qh 182 62 57R 57r CD cord-320015-lbr2q4qh 182 63 ) ) -RRB- cord-320015-lbr2q4qh 182 64 , , , cord-320015-lbr2q4qh 182 65 a a DT cord-320015-lbr2q4qh 182 66 putative putative JJ cord-320015-lbr2q4qh 182 67 RAD2-like rad2-like NN cord-320015-lbr2q4qh 182 68 DNA dna NN cord-320015-lbr2q4qh 182 69 repair repair NN cord-320015-lbr2q4qh 182 70 protein protein NN cord-320015-lbr2q4qh 182 71 ( ( -LRB- cord-320015-lbr2q4qh 182 72 ORF ORF NNP cord-320015-lbr2q4qh 182 73 95R 95r CD cord-320015-lbr2q4qh 182 74 ) ) -RRB- cord-320015-lbr2q4qh 182 75 , , , cord-320015-lbr2q4qh 182 76 a a DT cord-320015-lbr2q4qh 182 77 129 129 CD cord-320015-lbr2q4qh 182 78 kDa kDa NNP cord-320015-lbr2q4qh 182 79 protein protein NN cord-320015-lbr2q4qh 182 80 of of IN cord-320015-lbr2q4qh 182 81 unknown unknown JJ cord-320015-lbr2q4qh 182 82 function function NN cord-320015-lbr2q4qh 182 83 ( ( -LRB- cord-320015-lbr2q4qh 182 84 ORF ORF NNP cord-320015-lbr2q4qh 182 85 41R 41r CD cord-320015-lbr2q4qh 182 86 ) ) -RRB- cord-320015-lbr2q4qh 182 87 , , , cord-320015-lbr2q4qh 182 88 and and CC cord-320015-lbr2q4qh 182 89 the the DT cord-320015-lbr2q4qh 182 90 viral viral JJ cord-320015-lbr2q4qh 182 91 DMTase DMTase NNP cord-320015-lbr2q4qh 182 92 ( ( -LRB- cord-320015-lbr2q4qh 182 93 ORF ORF NNP cord-320015-lbr2q4qh 182 94 83R 83R , cord-320015-lbr2q4qh 182 95 ) ) -RRB- cord-320015-lbr2q4qh 182 96 are be VBP cord-320015-lbr2q4qh 182 97 ongoing ongoing JJ cord-320015-lbr2q4qh 182 98 and and CC cord-320015-lbr2q4qh 182 99 have have VBP cord-320015-lbr2q4qh 182 100 demonstrated demonstrate VBN cord-320015-lbr2q4qh 182 101 reductions reduction NNS cord-320015-lbr2q4qh 182 102 in in IN cord-320015-lbr2q4qh 182 103 viral viral JJ cord-320015-lbr2q4qh 182 104 yields yield NNS cord-320015-lbr2q4qh 182 105 ranging range VBG cord-320015-lbr2q4qh 182 106 from from IN cord-320015-lbr2q4qh 182 107 41 41 CD cord-320015-lbr2q4qh 182 108 - - SYM cord-320015-lbr2q4qh 182 109 92 92 CD cord-320015-lbr2q4qh 182 110 % % NN cord-320015-lbr2q4qh 182 111 [ [ -LRB- cord-320015-lbr2q4qh 182 112 123 123 CD cord-320015-lbr2q4qh 182 113 ] ] -RRB- cord-320015-lbr2q4qh 182 114 . . . cord-320015-lbr2q4qh 183 1 However however RB cord-320015-lbr2q4qh 183 2 , , , cord-320015-lbr2q4qh 183 3 unlike unlike IN cord-320015-lbr2q4qh 183 4 the the DT cord-320015-lbr2q4qh 183 5 first first JJ cord-320015-lbr2q4qh 183 6 study study NN cord-320015-lbr2q4qh 183 7 in in IN cord-320015-lbr2q4qh 183 8 which which WDT cord-320015-lbr2q4qh 183 9 KD KD NNP cord-320015-lbr2q4qh 183 10 of of IN cord-320015-lbr2q4qh 183 11 MCP MCP NNP cord-320015-lbr2q4qh 183 12 , , , cord-320015-lbr2q4qh 183 13 vPOL vpol JJ cord-320015-lbr2q4qh 183 14 - - JJ cord-320015-lbr2q4qh 183 15 IIα iiα JJ cord-320015-lbr2q4qh 183 16 , , , cord-320015-lbr2q4qh 183 17 and and CC cord-320015-lbr2q4qh 183 18 18 18 CD cord-320015-lbr2q4qh 183 19 K K NNP cord-320015-lbr2q4qh 183 20 was be VBD cord-320015-lbr2q4qh 183 21 confirmed confirm VBN cord-320015-lbr2q4qh 183 22 by by IN cord-320015-lbr2q4qh 183 23 1D 1D NNP cord-320015-lbr2q4qh 183 24 SDS SDS NNP cord-320015-lbr2q4qh 183 25 - - HYPH cord-320015-lbr2q4qh 183 26 polyacrylamide polyacrylamide NN cord-320015-lbr2q4qh 183 27 gel gel NN cord-320015-lbr2q4qh 183 28 electrophoresis electrophoresis NN cord-320015-lbr2q4qh 183 29 , , , cord-320015-lbr2q4qh 183 30 we -PRON- PRP cord-320015-lbr2q4qh 183 31 have have VBP cord-320015-lbr2q4qh 183 32 been be VBN cord-320015-lbr2q4qh 183 33 unable unable JJ cord-320015-lbr2q4qh 183 34 to to TO cord-320015-lbr2q4qh 183 35 identify identify VB cord-320015-lbr2q4qh 183 36 unambiguously unambiguously RB cord-320015-lbr2q4qh 183 37 the the DT cord-320015-lbr2q4qh 183 38 targeted target VBN cord-320015-lbr2q4qh 183 39 protein protein NN cord-320015-lbr2q4qh 183 40 in in IN cord-320015-lbr2q4qh 183 41 these these DT cord-320015-lbr2q4qh 183 42 latest late JJS cord-320015-lbr2q4qh 183 43 studies study NNS cord-320015-lbr2q4qh 183 44 , , , cord-320015-lbr2q4qh 183 45 and and CC cord-320015-lbr2q4qh 183 46 thus thus RB cord-320015-lbr2q4qh 183 47 do do VBP cord-320015-lbr2q4qh 183 48 not not RB cord-320015-lbr2q4qh 183 49 know know VB cord-320015-lbr2q4qh 183 50 if if IN cord-320015-lbr2q4qh 183 51 the the DT cord-320015-lbr2q4qh 183 52 partial partial JJ cord-320015-lbr2q4qh 183 53 reductions reduction NNS cord-320015-lbr2q4qh 183 54 in in IN cord-320015-lbr2q4qh 183 55 virus virus NN cord-320015-lbr2q4qh 183 56 yields yield NNS cord-320015-lbr2q4qh 183 57 reflect reflect VBP cord-320015-lbr2q4qh 183 58 the the DT cord-320015-lbr2q4qh 183 59 non non JJ cord-320015-lbr2q4qh 183 60 - - JJ cord-320015-lbr2q4qh 183 61 essential essential JJ cord-320015-lbr2q4qh 183 62 nature nature NN cord-320015-lbr2q4qh 183 63 of of IN cord-320015-lbr2q4qh 183 64 the the DT cord-320015-lbr2q4qh 183 65 gene gene NN cord-320015-lbr2q4qh 183 66 product product NN cord-320015-lbr2q4qh 183 67 or or CC cord-320015-lbr2q4qh 183 68 incomplete incomplete JJ cord-320015-lbr2q4qh 183 69 KD kd NN cord-320015-lbr2q4qh 183 70 of of IN cord-320015-lbr2q4qh 183 71 the the DT cord-320015-lbr2q4qh 183 72 targeted target VBN cord-320015-lbr2q4qh 183 73 protein protein NN cord-320015-lbr2q4qh 183 74 . . . cord-320015-lbr2q4qh 184 1 To to TO cord-320015-lbr2q4qh 184 2 overcome overcome VB cord-320015-lbr2q4qh 184 3 this this DT cord-320015-lbr2q4qh 184 4 problem problem NN cord-320015-lbr2q4qh 184 5 , , , cord-320015-lbr2q4qh 184 6 recombinant recombinant JJ cord-320015-lbr2q4qh 184 7 viral viral JJ cord-320015-lbr2q4qh 184 8 proteins protein NNS cord-320015-lbr2q4qh 184 9 will will MD cord-320015-lbr2q4qh 184 10 be be VB cord-320015-lbr2q4qh 184 11 generated generate VBN cord-320015-lbr2q4qh 184 12 and and CC cord-320015-lbr2q4qh 184 13 used use VBN cord-320015-lbr2q4qh 184 14 to to TO cord-320015-lbr2q4qh 184 15 produce produce VB cord-320015-lbr2q4qh 184 16 polyclonal polyclonal JJ cord-320015-lbr2q4qh 184 17 antibodies antibody NNS cord-320015-lbr2q4qh 184 18 for for IN cord-320015-lbr2q4qh 184 19 Western western JJ cord-320015-lbr2q4qh 184 20 blot blot NN cord-320015-lbr2q4qh 184 21 and and CC cord-320015-lbr2q4qh 184 22 immunofluorescent immunofluorescent NN cord-320015-lbr2q4qh 184 23 analyses analysis NNS cord-320015-lbr2q4qh 184 24 . . . cord-320015-lbr2q4qh 185 1 Lastly lastly RB cord-320015-lbr2q4qh 185 2 , , , cord-320015-lbr2q4qh 185 3 although although IN cord-320015-lbr2q4qh 185 4 asMOs asMOs NNP cord-320015-lbr2q4qh 185 5 can can MD cord-320015-lbr2q4qh 185 6 be be VB cord-320015-lbr2q4qh 185 7 powerful powerful JJ cord-320015-lbr2q4qh 185 8 tools tool NNS cord-320015-lbr2q4qh 185 9 for for IN cord-320015-lbr2q4qh 185 10 uncovering uncover VBG cord-320015-lbr2q4qh 185 11 viral viral JJ cord-320015-lbr2q4qh 185 12 gene gene NN cord-320015-lbr2q4qh 185 13 function function NN cord-320015-lbr2q4qh 185 14 , , , cord-320015-lbr2q4qh 185 15 their -PRON- PRP$ cord-320015-lbr2q4qh 185 16 use use NN cord-320015-lbr2q4qh 185 17 is be VBZ cord-320015-lbr2q4qh 185 18 potentially potentially RB cord-320015-lbr2q4qh 185 19 limited limit VBN cord-320015-lbr2q4qh 185 20 by by IN cord-320015-lbr2q4qh 185 21 the the DT cord-320015-lbr2q4qh 185 22 nature nature NN cord-320015-lbr2q4qh 185 23 of of IN cord-320015-lbr2q4qh 185 24 some some DT cord-320015-lbr2q4qh 185 25 ranavirus ranavirus NNP cord-320015-lbr2q4qh 185 26 mRNAs mrnas CD cord-320015-lbr2q4qh 185 27 . . . cord-320015-lbr2q4qh 186 1 Because because IN cord-320015-lbr2q4qh 186 2 many many JJ cord-320015-lbr2q4qh 186 3 FV3 FV3 NNP cord-320015-lbr2q4qh 186 4 transcripts transcript NNS cord-320015-lbr2q4qh 186 5 possess possess VBP cord-320015-lbr2q4qh 186 6 very very RB cord-320015-lbr2q4qh 186 7 short short JJ cord-320015-lbr2q4qh 186 8 , , , cord-320015-lbr2q4qh 186 9 AU AU NNP cord-320015-lbr2q4qh 186 10 - - HYPH cord-320015-lbr2q4qh 186 11 rich rich JJ cord-320015-lbr2q4qh 186 12 5 5 CD cord-320015-lbr2q4qh 186 13 ' ' '' cord-320015-lbr2q4qh 186 14 non non JJ cord-320015-lbr2q4qh 186 15 - - JJ cord-320015-lbr2q4qh 186 16 translated translate VBN cord-320015-lbr2q4qh 186 17 regions region NNS cord-320015-lbr2q4qh 186 18 , , , cord-320015-lbr2q4qh 186 19 and and CC cord-320015-lbr2q4qh 186 20 because because IN cord-320015-lbr2q4qh 186 21 sequences sequence NNS cord-320015-lbr2q4qh 186 22 surrounding surround VBG cord-320015-lbr2q4qh 186 23 the the DT cord-320015-lbr2q4qh 186 24 AUG AUG NNP cord-320015-lbr2q4qh 186 25 initiation initiation NN cord-320015-lbr2q4qh 186 26 codon codon NN cord-320015-lbr2q4qh 186 27 may may MD cord-320015-lbr2q4qh 186 28 be be VB cord-320015-lbr2q4qh 186 29 unfavorable unfavorable JJ cord-320015-lbr2q4qh 186 30 for for IN cord-320015-lbr2q4qh 186 31 targeting target VBG cord-320015-lbr2q4qh 186 32 due due IN cord-320015-lbr2q4qh 186 33 to to IN cord-320015-lbr2q4qh 186 34 , , , cord-320015-lbr2q4qh 186 35 for for IN cord-320015-lbr2q4qh 186 36 example example NN cord-320015-lbr2q4qh 186 37 , , , cord-320015-lbr2q4qh 186 38 high high JJ cord-320015-lbr2q4qh 186 39 AU AU NNP cord-320015-lbr2q4qh 186 40 content content NN cord-320015-lbr2q4qh 186 41 or or CC cord-320015-lbr2q4qh 186 42 secondary secondary JJ cord-320015-lbr2q4qh 186 43 structure structure NN cord-320015-lbr2q4qh 186 44 , , , cord-320015-lbr2q4qh 186 45 asMO asmo NN cord-320015-lbr2q4qh 186 46 - - HYPH cord-320015-lbr2q4qh 186 47 mediated mediate VBN cord-320015-lbr2q4qh 186 48 KD KD NNP cord-320015-lbr2q4qh 186 49 approaches approach NNS cord-320015-lbr2q4qh 186 50 may may MD cord-320015-lbr2q4qh 186 51 not not RB cord-320015-lbr2q4qh 186 52 succeed succeed VB cord-320015-lbr2q4qh 186 53 with with IN cord-320015-lbr2q4qh 186 54 all all DT cord-320015-lbr2q4qh 186 55 targeted target VBN cord-320015-lbr2q4qh 186 56 genes gene NNS cord-320015-lbr2q4qh 186 57 . . . cord-320015-lbr2q4qh 187 1 In in IN cord-320015-lbr2q4qh 187 2 these these DT cord-320015-lbr2q4qh 187 3 cases case NNS cord-320015-lbr2q4qh 187 4 , , , cord-320015-lbr2q4qh 187 5 siRNA sirna NN cord-320015-lbr2q4qh 187 6 - - HYPH cord-320015-lbr2q4qh 187 7 mediated mediate VBN cord-320015-lbr2q4qh 187 8 KD KD NNP cord-320015-lbr2q4qh 187 9 or or CC cord-320015-lbr2q4qh 187 10 KO KO NNP cord-320015-lbr2q4qh 187 11 approaches approach NNS cord-320015-lbr2q4qh 187 12 , , , cord-320015-lbr2q4qh 187 13 discussed discuss VBN cord-320015-lbr2q4qh 187 14 below below RB cord-320015-lbr2q4qh 187 15 , , , cord-320015-lbr2q4qh 187 16 provide provide VB cord-320015-lbr2q4qh 187 17 alternative alternative JJ cord-320015-lbr2q4qh 187 18 approaches approach NNS cord-320015-lbr2q4qh 187 19 . . . cord-320015-lbr2q4qh 188 1 Nevertheless nevertheless RB cord-320015-lbr2q4qh 188 2 , , , cord-320015-lbr2q4qh 188 3 despite despite IN cord-320015-lbr2q4qh 188 4 these these DT cord-320015-lbr2q4qh 188 5 limitations limitation NNS cord-320015-lbr2q4qh 188 6 , , , cord-320015-lbr2q4qh 188 7 asMOs asmos ADD cord-320015-lbr2q4qh 188 8 have have VBP cord-320015-lbr2q4qh 188 9 provided provide VBN cord-320015-lbr2q4qh 188 10 insight insight NN cord-320015-lbr2q4qh 188 11 into into IN cord-320015-lbr2q4qh 188 12 the the DT cord-320015-lbr2q4qh 188 13 roles role NNS cord-320015-lbr2q4qh 188 14 of of IN cord-320015-lbr2q4qh 188 15 several several JJ cord-320015-lbr2q4qh 188 16 FV3 FV3 NNP cord-320015-lbr2q4qh 188 17 genes gene NNS cord-320015-lbr2q4qh 188 18 and and CC cord-320015-lbr2q4qh 188 19 will will MD cord-320015-lbr2q4qh 188 20 continue continue VB cord-320015-lbr2q4qh 188 21 to to TO cord-320015-lbr2q4qh 188 22 be be VB cord-320015-lbr2q4qh 188 23 a a DT cord-320015-lbr2q4qh 188 24 useful useful JJ cord-320015-lbr2q4qh 188 25 tool tool NN cord-320015-lbr2q4qh 188 26 for for IN cord-320015-lbr2q4qh 188 27 elucidating elucidate VBG cord-320015-lbr2q4qh 188 28 viral viral JJ cord-320015-lbr2q4qh 188 29 gene gene NN cord-320015-lbr2q4qh 188 30 function function NN cord-320015-lbr2q4qh 188 31 in in IN cord-320015-lbr2q4qh 188 32 vitro vitro FW cord-320015-lbr2q4qh 188 33 . . . cord-320015-lbr2q4qh 189 1 Together together RB cord-320015-lbr2q4qh 189 2 with with IN cord-320015-lbr2q4qh 189 3 anti anti JJ cord-320015-lbr2q4qh 189 4 - - JJ cord-320015-lbr2q4qh 189 5 viral viral JJ cord-320015-lbr2q4qh 189 6 antibodies antibody NNS cord-320015-lbr2q4qh 189 7 to to TO cord-320015-lbr2q4qh 189 8 confirm confirm VB cord-320015-lbr2q4qh 189 9 knock knock VB cord-320015-lbr2q4qh 189 10 down down RP cord-320015-lbr2q4qh 189 11 and and CC cord-320015-lbr2q4qh 189 12 identify identify VB cord-320015-lbr2q4qh 189 13 intracellular intracellular JJ cord-320015-lbr2q4qh 189 14 locations location NNS cord-320015-lbr2q4qh 189 15 , , , cord-320015-lbr2q4qh 189 16 these these DT cord-320015-lbr2q4qh 189 17 studies study NNS cord-320015-lbr2q4qh 189 18 will will MD cord-320015-lbr2q4qh 189 19 broaden broaden VB cord-320015-lbr2q4qh 189 20 our -PRON- PRP$ cord-320015-lbr2q4qh 189 21 understanding understanding NN cord-320015-lbr2q4qh 189 22 of of IN cord-320015-lbr2q4qh 189 23 the the DT cord-320015-lbr2q4qh 189 24 complex complex JJ cord-320015-lbr2q4qh 189 25 story story NN cord-320015-lbr2q4qh 189 26 of of IN cord-320015-lbr2q4qh 189 27 virus virus NN cord-320015-lbr2q4qh 189 28 - - HYPH cord-320015-lbr2q4qh 189 29 host host NN cord-320015-lbr2q4qh 189 30 interactions interaction NNS cord-320015-lbr2q4qh 189 31 . . . cord-320015-lbr2q4qh 190 1 As as IN cord-320015-lbr2q4qh 190 2 an an DT cord-320015-lbr2q4qh 190 3 alternative alternative NN cord-320015-lbr2q4qh 190 4 to to IN cord-320015-lbr2q4qh 190 5 asMOs asmos CD cord-320015-lbr2q4qh 190 6 , , , cord-320015-lbr2q4qh 190 7 siRNAs siRNAs NNP cord-320015-lbr2q4qh 190 8 have have VBP cord-320015-lbr2q4qh 190 9 also also RB cord-320015-lbr2q4qh 190 10 been be VBN cord-320015-lbr2q4qh 190 11 used use VBN cord-320015-lbr2q4qh 190 12 in in IN cord-320015-lbr2q4qh 190 13 a a DT cord-320015-lbr2q4qh 190 14 limited limited JJ cord-320015-lbr2q4qh 190 15 number number NN cord-320015-lbr2q4qh 190 16 of of IN cord-320015-lbr2q4qh 190 17 cases case NNS cord-320015-lbr2q4qh 190 18 to to TO cord-320015-lbr2q4qh 190 19 knock knock VB cord-320015-lbr2q4qh 190 20 down down RP cord-320015-lbr2q4qh 190 21 iridovirid iridovirid NNP cord-320015-lbr2q4qh 190 22 gene gene NN cord-320015-lbr2q4qh 190 23 expression expression NN cord-320015-lbr2q4qh 190 24 [ [ -LRB- cord-320015-lbr2q4qh 190 25 48 48 CD cord-320015-lbr2q4qh 190 26 , , , cord-320015-lbr2q4qh 190 27 122 122 CD cord-320015-lbr2q4qh 190 28 , , , cord-320015-lbr2q4qh 190 29 124 124 CD cord-320015-lbr2q4qh 190 30 , , , cord-320015-lbr2q4qh 190 31 125 125 CD cord-320015-lbr2q4qh 190 32 ] ] -RRB- cord-320015-lbr2q4qh 190 33 . . . cord-320015-lbr2q4qh 191 1 While while IN cord-320015-lbr2q4qh 191 2 knock knock VB cord-320015-lbr2q4qh 191 3 down down RP cord-320015-lbr2q4qh 191 4 has have VBZ cord-320015-lbr2q4qh 191 5 been be VBN cord-320015-lbr2q4qh 191 6 achieved achieve VBN cord-320015-lbr2q4qh 191 7 , , , cord-320015-lbr2q4qh 191 8 we -PRON- PRP cord-320015-lbr2q4qh 191 9 observed observe VBD cord-320015-lbr2q4qh 191 10 that that IN cord-320015-lbr2q4qh 191 11 inhibition inhibition NN cord-320015-lbr2q4qh 191 12 of of IN cord-320015-lbr2q4qh 191 13 FV3 FV3 NNP cord-320015-lbr2q4qh 191 14 gene gene NN cord-320015-lbr2q4qh 191 15 expression expression NN cord-320015-lbr2q4qh 191 16 only only RB cord-320015-lbr2q4qh 191 17 took take VBD cord-320015-lbr2q4qh 191 18 place place NN cord-320015-lbr2q4qh 191 19 if if IN cord-320015-lbr2q4qh 191 20 cells cell NNS cord-320015-lbr2q4qh 191 21 were be VBD cord-320015-lbr2q4qh 191 22 infected infect VBN cord-320015-lbr2q4qh 191 23 at at IN cord-320015-lbr2q4qh 191 24 low low JJ cord-320015-lbr2q4qh 191 25 multiplicities multiplicity NNS cord-320015-lbr2q4qh 191 26 of of IN cord-320015-lbr2q4qh 191 27 infection infection NN cord-320015-lbr2q4qh 191 28 , , , cord-320015-lbr2q4qh 191 29 i.e. i.e. FW cord-320015-lbr2q4qh 191 30 , , , cord-320015-lbr2q4qh 191 31 < < XX cord-320015-lbr2q4qh 191 32 0.1 0.1 CD cord-320015-lbr2q4qh 191 33 PFU pfu NN cord-320015-lbr2q4qh 191 34 / / SYM cord-320015-lbr2q4qh 191 35 cell cell NN cord-320015-lbr2q4qh 191 36 . . . cord-320015-lbr2q4qh 192 1 Similar similar JJ cord-320015-lbr2q4qh 192 2 to to IN cord-320015-lbr2q4qh 192 3 the the DT cord-320015-lbr2q4qh 192 4 situation situation NN cord-320015-lbr2q4qh 192 5 in in IN cord-320015-lbr2q4qh 192 6 some some DT cord-320015-lbr2q4qh 192 7 other other JJ cord-320015-lbr2q4qh 192 8 viral viral JJ cord-320015-lbr2q4qh 192 9 systems system NNS cord-320015-lbr2q4qh 192 10 , , , cord-320015-lbr2q4qh 192 11 the the DT cord-320015-lbr2q4qh 192 12 inverse inverse JJ cord-320015-lbr2q4qh 192 13 relationship relationship NN cord-320015-lbr2q4qh 192 14 between between IN cord-320015-lbr2q4qh 192 15 multiplicity multiplicity NN cord-320015-lbr2q4qh 192 16 of of IN cord-320015-lbr2q4qh 192 17 infection infection NN cord-320015-lbr2q4qh 192 18 and and CC cord-320015-lbr2q4qh 192 19 siRNA siRNA NNP cord-320015-lbr2q4qh 192 20 knockdown knockdown NNP cord-320015-lbr2q4qh 192 21 suggests suggest VBZ cord-320015-lbr2q4qh 192 22 that that IN cord-320015-lbr2q4qh 192 23 a a DT cord-320015-lbr2q4qh 192 24 virion virion NN cord-320015-lbr2q4qh 192 25 - - HYPH cord-320015-lbr2q4qh 192 26 associated associate VBN cord-320015-lbr2q4qh 192 27 or or CC cord-320015-lbr2q4qh 192 28 virus virus NN cord-320015-lbr2q4qh 192 29 - - HYPH cord-320015-lbr2q4qh 192 30 encoded encode VBN cord-320015-lbr2q4qh 192 31 protein protein NN cord-320015-lbr2q4qh 192 32 may may MD cord-320015-lbr2q4qh 192 33 block block VB cord-320015-lbr2q4qh 192 34 siRNA siRNA NNP cord-320015-lbr2q4qh 192 35 - - HYPH cord-320015-lbr2q4qh 192 36 mediated mediate VBN cord-320015-lbr2q4qh 192 37 knockdown knockdown NN cord-320015-lbr2q4qh 192 38 by by IN cord-320015-lbr2q4qh 192 39 either either DT cord-320015-lbr2q4qh 192 40 binding bind VBG cord-320015-lbr2q4qh 192 41 or or CC cord-320015-lbr2q4qh 192 42 degrading degrade VBG cord-320015-lbr2q4qh 192 43 siRNA siRNA NNP cord-320015-lbr2q4qh 192 44 [ [ -LRB- cord-320015-lbr2q4qh 192 45 126 126 CD cord-320015-lbr2q4qh 192 46 ] ] -RRB- cord-320015-lbr2q4qh 192 47 . . . cord-320015-lbr2q4qh 193 1 To to IN cord-320015-lbr2q4qh 193 2 date date NN cord-320015-lbr2q4qh 193 3 , , , cord-320015-lbr2q4qh 193 4 expression expression NN cord-320015-lbr2q4qh 193 5 of of IN cord-320015-lbr2q4qh 193 6 FV3 FV3 NNP cord-320015-lbr2q4qh 193 7 transcripts transcript NNS cord-320015-lbr2q4qh 193 8 encoding encode VBG cord-320015-lbr2q4qh 193 9 MCP MCP NNP cord-320015-lbr2q4qh 193 10 , , , cord-320015-lbr2q4qh 193 11 DMTase DMTase NNP cord-320015-lbr2q4qh 193 12 , , , cord-320015-lbr2q4qh 193 13 and and CC cord-320015-lbr2q4qh 193 14 vPOL vpol JJ cord-320015-lbr2q4qh 193 15 - - JJ cord-320015-lbr2q4qh 193 16 IIα iiα JJ cord-320015-lbr2q4qh 193 17 have have VBP cord-320015-lbr2q4qh 193 18 been be VBN cord-320015-lbr2q4qh 193 19 knocked knock VBN cord-320015-lbr2q4qh 193 20 down down RP cord-320015-lbr2q4qh 193 21 by by IN cord-320015-lbr2q4qh 193 22 siRNA siRNA NNP cord-320015-lbr2q4qh 193 23 and and CC cord-320015-lbr2q4qh 193 24 resulted result VBD cord-320015-lbr2q4qh 193 25 in in IN cord-320015-lbr2q4qh 193 26 a a DT cord-320015-lbr2q4qh 193 27 marked marked JJ cord-320015-lbr2q4qh 193 28 inhibition inhibition NN cord-320015-lbr2q4qh 193 29 of of IN cord-320015-lbr2q4qh 193 30 replication replication NN cord-320015-lbr2q4qh 193 31 . . . cord-320015-lbr2q4qh 194 1 A a DT cord-320015-lbr2q4qh 194 2 powerful powerful JJ cord-320015-lbr2q4qh 194 3 and and CC cord-320015-lbr2q4qh 194 4 potentially potentially RB cord-320015-lbr2q4qh 194 5 more more RBR cord-320015-lbr2q4qh 194 6 useful useful JJ cord-320015-lbr2q4qh 194 7 approach approach NN cord-320015-lbr2q4qh 194 8 to to TO cord-320015-lbr2q4qh 194 9 elucidate elucidate VB cord-320015-lbr2q4qh 194 10 viral viral JJ cord-320015-lbr2q4qh 194 11 gene gene NN cord-320015-lbr2q4qh 194 12 function function NN cord-320015-lbr2q4qh 194 13 involves involve VBZ cord-320015-lbr2q4qh 194 14 the the DT cord-320015-lbr2q4qh 194 15 generation generation NN cord-320015-lbr2q4qh 194 16 of of IN cord-320015-lbr2q4qh 194 17 knock knock VB cord-320015-lbr2q4qh 194 18 out out RP cord-320015-lbr2q4qh 194 19 ( ( -LRB- cord-320015-lbr2q4qh 194 20 KO KO NNP cord-320015-lbr2q4qh 194 21 ) ) -RRB- cord-320015-lbr2q4qh 194 22 mutants mutant NNS cord-320015-lbr2q4qh 194 23 since since IN cord-320015-lbr2q4qh 194 24 , , , cord-320015-lbr2q4qh 194 25 in in IN cord-320015-lbr2q4qh 194 26 contrast contrast NN cord-320015-lbr2q4qh 194 27 to to IN cord-320015-lbr2q4qh 194 28 transient transient JJ cord-320015-lbr2q4qh 194 29 KD KD NNP cord-320015-lbr2q4qh 194 30 triggered trigger VBN cord-320015-lbr2q4qh 194 31 by by IN cord-320015-lbr2q4qh 194 32 asMOs asMOs NNP cord-320015-lbr2q4qh 194 33 or or CC cord-320015-lbr2q4qh 194 34 siRNAs siRNAs NNP cord-320015-lbr2q4qh 194 35 , , , cord-320015-lbr2q4qh 194 36 the the DT cord-320015-lbr2q4qh 194 37 phenotypes phenotype NNS cord-320015-lbr2q4qh 194 38 of of IN cord-320015-lbr2q4qh 194 39 KO KO NNP cord-320015-lbr2q4qh 194 40 mutants mutant NNS cord-320015-lbr2q4qh 194 41 can can MD cord-320015-lbr2q4qh 194 42 be be VB cord-320015-lbr2q4qh 194 43 evaluated evaluate VBN cord-320015-lbr2q4qh 194 44 both both CC cord-320015-lbr2q4qh 194 45 in in IN cord-320015-lbr2q4qh 194 46 vitro vitro FW cord-320015-lbr2q4qh 194 47 and and CC cord-320015-lbr2q4qh 194 48 in in IN cord-320015-lbr2q4qh 194 49 vivo vivo NNP cord-320015-lbr2q4qh 194 50 . . . cord-320015-lbr2q4qh 195 1 This this DT cord-320015-lbr2q4qh 195 2 feature feature NN cord-320015-lbr2q4qh 195 3 makes make VBZ cord-320015-lbr2q4qh 195 4 KO KO NNP cord-320015-lbr2q4qh 195 5 mutants mutant NNS cord-320015-lbr2q4qh 195 6 especially especially RB cord-320015-lbr2q4qh 195 7 useful useful JJ cord-320015-lbr2q4qh 195 8 for for IN cord-320015-lbr2q4qh 195 9 identifying identify VBG cord-320015-lbr2q4qh 195 10 viral viral JJ cord-320015-lbr2q4qh 195 11 genes gene NNS cord-320015-lbr2q4qh 195 12 that that WDT cord-320015-lbr2q4qh 195 13 play play VBP cord-320015-lbr2q4qh 195 14 roles role NNS cord-320015-lbr2q4qh 195 15 in in IN cord-320015-lbr2q4qh 195 16 immune immune JJ cord-320015-lbr2q4qh 195 17 evasion evasion NN cord-320015-lbr2q4qh 195 18 and and CC cord-320015-lbr2q4qh 195 19 virulence virulence NN cord-320015-lbr2q4qh 195 20 . . . cord-320015-lbr2q4qh 196 1 KO KO NNP cord-320015-lbr2q4qh 196 2 mutants mutant NNS cord-320015-lbr2q4qh 196 3 have have VBP cord-320015-lbr2q4qh 196 4 been be VBN cord-320015-lbr2q4qh 196 5 used use VBN cord-320015-lbr2q4qh 196 6 extensively extensively RB cord-320015-lbr2q4qh 196 7 to to TO cord-320015-lbr2q4qh 196 8 elucidate elucidate VB cord-320015-lbr2q4qh 196 9 the the DT cord-320015-lbr2q4qh 196 10 function function NN cord-320015-lbr2q4qh 196 11 of of IN cord-320015-lbr2q4qh 196 12 various various JJ cord-320015-lbr2q4qh 196 13 poxviruses poxvirus NNS cord-320015-lbr2q4qh 196 14 genes gene NNS cord-320015-lbr2q4qh 196 15 [ [ -LRB- cord-320015-lbr2q4qh 196 16 127 127 CD cord-320015-lbr2q4qh 196 17 ] ] -RRB- cord-320015-lbr2q4qh 196 18 [ [ -LRB- cord-320015-lbr2q4qh 196 19 128 128 CD cord-320015-lbr2q4qh 196 20 ] ] -RRB- cord-320015-lbr2q4qh 196 21 [ [ -LRB- cord-320015-lbr2q4qh 196 22 129 129 CD cord-320015-lbr2q4qh 196 23 ] ] -RRB- cord-320015-lbr2q4qh 196 24 , , , cord-320015-lbr2q4qh 196 25 and and CC cord-320015-lbr2q4qh 196 26 methodology methodology NN cord-320015-lbr2q4qh 196 27 developed develop VBN cord-320015-lbr2q4qh 196 28 here here RB cord-320015-lbr2q4qh 196 29 has have VBZ cord-320015-lbr2q4qh 196 30 been be VBN cord-320015-lbr2q4qh 196 31 adapted adapt VBN cord-320015-lbr2q4qh 196 32 to to IN cord-320015-lbr2q4qh 196 33 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 196 34 . . . cord-320015-lbr2q4qh 197 1 However however RB cord-320015-lbr2q4qh 197 2 , , , cord-320015-lbr2q4qh 197 3 despite despite IN cord-320015-lbr2q4qh 197 4 using use VBG cord-320015-lbr2q4qh 197 5 poxviruses poxvirus NNS cord-320015-lbr2q4qh 197 6 as as IN cord-320015-lbr2q4qh 197 7 a a DT cord-320015-lbr2q4qh 197 8 guide guide NN cord-320015-lbr2q4qh 197 9 , , , cord-320015-lbr2q4qh 197 10 ranavirus ranavirus NNP cord-320015-lbr2q4qh 197 11 KO KO NNP cord-320015-lbr2q4qh 197 12 mutants mutant NNS cord-320015-lbr2q4qh 197 13 , , , cord-320015-lbr2q4qh 197 14 using use VBG cord-320015-lbr2q4qh 197 15 homologous homologous JJ cord-320015-lbr2q4qh 197 16 recombination recombination NN cord-320015-lbr2q4qh 197 17 to to TO cord-320015-lbr2q4qh 197 18 replace replace VB cord-320015-lbr2q4qh 197 19 the the DT cord-320015-lbr2q4qh 197 20 targeted target VBN cord-320015-lbr2q4qh 197 21 gene gene NN cord-320015-lbr2q4qh 197 22 with with IN cord-320015-lbr2q4qh 197 23 a a DT cord-320015-lbr2q4qh 197 24 selectable selectable JJ cord-320015-lbr2q4qh 197 25 marker marker NN cord-320015-lbr2q4qh 197 26 , , , cord-320015-lbr2q4qh 197 27 have have VBP cord-320015-lbr2q4qh 197 28 only only RB cord-320015-lbr2q4qh 197 29 recently recently RB cord-320015-lbr2q4qh 197 30 been be VBN cord-320015-lbr2q4qh 197 31 isolated isolate VBN cord-320015-lbr2q4qh 197 32 . . . cord-320015-lbr2q4qh 198 1 The the DT cord-320015-lbr2q4qh 198 2 reasons reason NNS cord-320015-lbr2q4qh 198 3 for for IN cord-320015-lbr2q4qh 198 4 the the DT cord-320015-lbr2q4qh 198 5 difficulty difficulty NN cord-320015-lbr2q4qh 198 6 in in IN cord-320015-lbr2q4qh 198 7 applying apply VBG cord-320015-lbr2q4qh 198 8 this this DT cord-320015-lbr2q4qh 198 9 approach approach NN cord-320015-lbr2q4qh 198 10 to to IN cord-320015-lbr2q4qh 198 11 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 198 12 are be VBP cord-320015-lbr2q4qh 198 13 not not RB cord-320015-lbr2q4qh 198 14 clear clear JJ cord-320015-lbr2q4qh 198 15 , , , cord-320015-lbr2q4qh 198 16 but but CC cord-320015-lbr2q4qh 198 17 may may MD cord-320015-lbr2q4qh 198 18 reflect reflect VB cord-320015-lbr2q4qh 198 19 the the DT cord-320015-lbr2q4qh 198 20 presence presence NN cord-320015-lbr2q4qh 198 21 of of IN cord-320015-lbr2q4qh 198 22 a a DT cord-320015-lbr2q4qh 198 23 ranavirus ranavirus NN cord-320015-lbr2q4qh 198 24 - - HYPH cord-320015-lbr2q4qh 198 25 encoded encode VBN cord-320015-lbr2q4qh 198 26 endonuclease endonuclease NN cord-320015-lbr2q4qh 198 27 that that WDT cord-320015-lbr2q4qh 198 28 cleaves cleave VBZ cord-320015-lbr2q4qh 198 29 unmethylated unmethylated JJ cord-320015-lbr2q4qh 198 30 DNA DNA NNP cord-320015-lbr2q4qh 198 31 [ [ -LRB- cord-320015-lbr2q4qh 198 32 30 30 CD cord-320015-lbr2q4qh 198 33 , , , cord-320015-lbr2q4qh 198 34 38 38 CD cord-320015-lbr2q4qh 198 35 ] ] -RRB- cord-320015-lbr2q4qh 198 36 . . . cord-320015-lbr2q4qh 199 1 If if IN cord-320015-lbr2q4qh 199 2 correct correct JJ cord-320015-lbr2q4qh 199 3 , , , cord-320015-lbr2q4qh 199 4 introduction introduction NN cord-320015-lbr2q4qh 199 5 of of IN cord-320015-lbr2q4qh 199 6 unmethylated unmethylated JJ cord-320015-lbr2q4qh 199 7 plasmid plasmid NN cord-320015-lbr2q4qh 199 8 DNA DNA NNP cord-320015-lbr2q4qh 199 9 , , , cord-320015-lbr2q4qh 199 10 bearing bear VBG cord-320015-lbr2q4qh 199 11 the the DT cord-320015-lbr2q4qh 199 12 selectable selectable JJ cord-320015-lbr2q4qh 199 13 marker marker NN cord-320015-lbr2q4qh 199 14 , , , cord-320015-lbr2q4qh 199 15 into into IN cord-320015-lbr2q4qh 199 16 a a DT cord-320015-lbr2q4qh 199 17 virus virus NN cord-320015-lbr2q4qh 199 18 - - HYPH cord-320015-lbr2q4qh 199 19 infected infect VBN cord-320015-lbr2q4qh 199 20 cell cell NN cord-320015-lbr2q4qh 199 21 may may MD cord-320015-lbr2q4qh 199 22 lead lead VB cord-320015-lbr2q4qh 199 23 to to IN cord-320015-lbr2q4qh 199 24 degradation degradation NN cord-320015-lbr2q4qh 199 25 of of IN cord-320015-lbr2q4qh 199 26 the the DT cord-320015-lbr2q4qh 199 27 majority majority NN cord-320015-lbr2q4qh 199 28 of of IN cord-320015-lbr2q4qh 199 29 plasmid plasmid NN cord-320015-lbr2q4qh 199 30 DNA DNA NNP cord-320015-lbr2q4qh 199 31 and and CC cord-320015-lbr2q4qh 199 32 make make VB cord-320015-lbr2q4qh 199 33 recombination recombination NN cord-320015-lbr2q4qh 199 34 unlikely unlikely JJ cord-320015-lbr2q4qh 199 35 . . . cord-320015-lbr2q4qh 200 1 As as IN cord-320015-lbr2q4qh 200 2 was be VBD cord-320015-lbr2q4qh 200 3 done do VBN cord-320015-lbr2q4qh 200 4 previously previously RB cord-320015-lbr2q4qh 200 5 in in IN cord-320015-lbr2q4qh 200 6 a a DT cord-320015-lbr2q4qh 200 7 recombinant recombinant JJ cord-320015-lbr2q4qh 200 8 BIV BIV NNP cord-320015-lbr2q4qh 200 9 vector vector NN cord-320015-lbr2q4qh 200 10 [ [ -LRB- cord-320015-lbr2q4qh 200 11 130 130 CD cord-320015-lbr2q4qh 200 12 , , , cord-320015-lbr2q4qh 200 13 131 131 CD cord-320015-lbr2q4qh 200 14 ] ] -RRB- cord-320015-lbr2q4qh 200 15 , , , cord-320015-lbr2q4qh 200 16 positioning position VBG cord-320015-lbr2q4qh 200 17 the the DT cord-320015-lbr2q4qh 200 18 selectable selectable JJ cord-320015-lbr2q4qh 200 19 marker marker NN cord-320015-lbr2q4qh 200 20 , , , cord-320015-lbr2q4qh 200 21 e.g. e.g. RB cord-320015-lbr2q4qh 200 22 , , , cord-320015-lbr2q4qh 200 23 the the DT cord-320015-lbr2q4qh 200 24 neomycin neomycin NN cord-320015-lbr2q4qh 200 25 - - HYPH cord-320015-lbr2q4qh 200 26 resistance resistance NN cord-320015-lbr2q4qh 200 27 gene gene NN cord-320015-lbr2q4qh 200 28 , , , cord-320015-lbr2q4qh 200 29 downstream downstream RB cord-320015-lbr2q4qh 200 30 of of IN cord-320015-lbr2q4qh 200 31 the the DT cord-320015-lbr2q4qh 200 32 promoter promoter NN cord-320015-lbr2q4qh 200 33 for for IN cord-320015-lbr2q4qh 200 34 the the DT cord-320015-lbr2q4qh 200 35 18 18 CD cord-320015-lbr2q4qh 200 36 K K NNP cord-320015-lbr2q4qh 200 37 immediate immediate JJ cord-320015-lbr2q4qh 200 38 early early JJ cord-320015-lbr2q4qh 200 39 ( ( -LRB- cord-320015-lbr2q4qh 200 40 IE IE NNP cord-320015-lbr2q4qh 200 41 ) ) -RRB- cord-320015-lbr2q4qh 200 42 gene gene NN cord-320015-lbr2q4qh 200 43 drove drive VBD cord-320015-lbr2q4qh 200 44 high high JJ cord-320015-lbr2q4qh 200 45 levels level NNS cord-320015-lbr2q4qh 200 46 of of IN cord-320015-lbr2q4qh 200 47 expression expression NN cord-320015-lbr2q4qh 200 48 of of IN cord-320015-lbr2q4qh 200 49 the the DT cord-320015-lbr2q4qh 200 50 resistance resistance NN cord-320015-lbr2q4qh 200 51 gene gene NN cord-320015-lbr2q4qh 200 52 and and CC cord-320015-lbr2q4qh 200 53 permitted permit VBN cord-320015-lbr2q4qh 200 54 isolation isolation NN cord-320015-lbr2q4qh 200 55 of of IN cord-320015-lbr2q4qh 200 56 rare rare JJ cord-320015-lbr2q4qh 200 57 recombinants recombinant NNS cord-320015-lbr2q4qh 200 58 . . . cord-320015-lbr2q4qh 201 1 The the DT cord-320015-lbr2q4qh 201 2 first first JJ cord-320015-lbr2q4qh 201 3 ranavirus ranavirus NNP cord-320015-lbr2q4qh 201 4 KO KO NNP cord-320015-lbr2q4qh 201 5 mutant mutant NN cord-320015-lbr2q4qh 201 6 targeted target VBD cord-320015-lbr2q4qh 201 7 the the DT cord-320015-lbr2q4qh 201 8 ATV ATV NNP cord-320015-lbr2q4qh 201 9 - - HYPH cord-320015-lbr2q4qh 201 10 encoded encode VBN cord-320015-lbr2q4qh 201 11 homolog homolog NN cord-320015-lbr2q4qh 201 12 of of IN cord-320015-lbr2q4qh 201 13 eukaryotic eukaryotic JJ cord-320015-lbr2q4qh 201 14 translational translational JJ cord-320015-lbr2q4qh 201 15 initiation initiation NN cord-320015-lbr2q4qh 201 16 factor factor NN cord-320015-lbr2q4qh 201 17 2α 2α CD cord-320015-lbr2q4qh 201 18 ( ( -LRB- cord-320015-lbr2q4qh 201 19 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 201 20 ) ) -RRB- cord-320015-lbr2q4qh 202 1 [ [ -LRB- cord-320015-lbr2q4qh 202 2 132 132 CD cord-320015-lbr2q4qh 202 3 ] ] -RRB- cord-320015-lbr2q4qh 202 4 . . . cord-320015-lbr2q4qh 203 1 As as IN cord-320015-lbr2q4qh 203 2 discussed discuss VBN cord-320015-lbr2q4qh 203 3 above above RB cord-320015-lbr2q4qh 203 4 , , , cord-320015-lbr2q4qh 203 5 following follow VBG cord-320015-lbr2q4qh 203 6 virus virus NN cord-320015-lbr2q4qh 203 7 infection infection NN cord-320015-lbr2q4qh 203 8 , , , cord-320015-lbr2q4qh 203 9 host host NN cord-320015-lbr2q4qh 203 10 cells cell NNS cord-320015-lbr2q4qh 203 11 activate activate VBP cord-320015-lbr2q4qh 203 12 PKR PKR NNP cord-320015-lbr2q4qh 203 13 , , , cord-320015-lbr2q4qh 203 14 an an DT cord-320015-lbr2q4qh 203 15 interferon interferon NN cord-320015-lbr2q4qh 203 16 ( ( -LRB- cord-320015-lbr2q4qh 203 17 IFN IFN NNP cord-320015-lbr2q4qh 203 18 ) ) -RRB- cord-320015-lbr2q4qh 203 19 inducible inducible JJ cord-320015-lbr2q4qh 203 20 double double RB cord-320015-lbr2q4qh 203 21 - - HYPH cord-320015-lbr2q4qh 203 22 stranded strand VBN cord-320015-lbr2q4qh 203 23 RNA RNA NNP cord-320015-lbr2q4qh 203 24 activated activate VBN cord-320015-lbr2q4qh 203 25 protein protein NN cord-320015-lbr2q4qh 203 26 kinase kinase NN cord-320015-lbr2q4qh 203 27 [ [ -LRB- cord-320015-lbr2q4qh 203 28 133 133 CD cord-320015-lbr2q4qh 203 29 ] ] -RRB- cord-320015-lbr2q4qh 203 30 , , , cord-320015-lbr2q4qh 203 31 that that IN cord-320015-lbr2q4qh 203 32 , , , cord-320015-lbr2q4qh 203 33 in in IN cord-320015-lbr2q4qh 203 34 turn turn NN cord-320015-lbr2q4qh 203 35 , , , cord-320015-lbr2q4qh 203 36 phosphorylates phosphorylate VBZ cord-320015-lbr2q4qh 203 37 the the DT cord-320015-lbr2q4qh 203 38 α α NNP cord-320015-lbr2q4qh 203 39 subunit subunit NN cord-320015-lbr2q4qh 203 40 of of IN cord-320015-lbr2q4qh 203 41 eIF-2 eIF-2 NNP cord-320015-lbr2q4qh 203 42 and and CC cord-320015-lbr2q4qh 203 43 , , , cord-320015-lbr2q4qh 203 44 as as IN cord-320015-lbr2q4qh 203 45 a a DT cord-320015-lbr2q4qh 203 46 consequence consequence NN cord-320015-lbr2q4qh 203 47 , , , cord-320015-lbr2q4qh 203 48 inhibits inhibit VBZ cord-320015-lbr2q4qh 203 49 both both DT cord-320015-lbr2q4qh 203 50 host host NN cord-320015-lbr2q4qh 203 51 and and CC cord-320015-lbr2q4qh 203 52 viral viral JJ cord-320015-lbr2q4qh 203 53 protein protein NN cord-320015-lbr2q4qh 203 54 synthesis synthesis NN cord-320015-lbr2q4qh 203 55 . . . cord-320015-lbr2q4qh 204 1 To to TO cord-320015-lbr2q4qh 204 2 prevent prevent VB cord-320015-lbr2q4qh 204 3 translational translational JJ cord-320015-lbr2q4qh 204 4 shut shut NN cord-320015-lbr2q4qh 204 5 - - HYPH cord-320015-lbr2q4qh 204 6 off off RP cord-320015-lbr2q4qh 204 7 , , , cord-320015-lbr2q4qh 204 8 several several JJ cord-320015-lbr2q4qh 204 9 viruses virus NNS cord-320015-lbr2q4qh 204 10 , , , cord-320015-lbr2q4qh 204 11 including include VBG cord-320015-lbr2q4qh 204 12 most most JJS cord-320015-lbr2q4qh 204 13 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 204 14 , , , cord-320015-lbr2q4qh 204 15 encode encode VB cord-320015-lbr2q4qh 204 16 an an DT cord-320015-lbr2q4qh 204 17 eIF-2α eif-2α JJ cord-320015-lbr2q4qh 204 18 homolog homolog NN cord-320015-lbr2q4qh 204 19 that that WDT cord-320015-lbr2q4qh 204 20 has have VBZ cord-320015-lbr2q4qh 204 21 been be VBN cord-320015-lbr2q4qh 204 22 proposed propose VBN cord-320015-lbr2q4qh 204 23 to to TO cord-320015-lbr2q4qh 204 24 function function VB cord-320015-lbr2q4qh 204 25 as as IN cord-320015-lbr2q4qh 204 26 a a DT cord-320015-lbr2q4qh 204 27 pseudosubstrate pseudosubstrate NN cord-320015-lbr2q4qh 204 28 for for IN cord-320015-lbr2q4qh 204 29 PKR PKR NNP cord-320015-lbr2q4qh 204 30 [ [ -LRB- cord-320015-lbr2q4qh 204 31 134 134 CD cord-320015-lbr2q4qh 204 32 ] ] -RRB- cord-320015-lbr2q4qh 204 33 . . . cord-320015-lbr2q4qh 205 1 In in IN cord-320015-lbr2q4qh 205 2 vaccinia vaccinia NN cord-320015-lbr2q4qh 205 3 virus virus NN cord-320015-lbr2q4qh 205 4 this this DT cord-320015-lbr2q4qh 205 5 homolog homolog NN cord-320015-lbr2q4qh 205 6 is be VBZ cord-320015-lbr2q4qh 205 7 designated designate VBN cord-320015-lbr2q4qh 205 8 K3L k3l CD cord-320015-lbr2q4qh 205 9 , , , cord-320015-lbr2q4qh 205 10 whereas whereas IN cord-320015-lbr2q4qh 205 11 in in IN cord-320015-lbr2q4qh 205 12 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 205 13 it -PRON- PRP cord-320015-lbr2q4qh 205 14 is be VBZ cord-320015-lbr2q4qh 205 15 termed term VBN cord-320015-lbr2q4qh 205 16 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 205 17 . . . cord-320015-lbr2q4qh 206 1 Although although IN cord-320015-lbr2q4qh 206 2 K3L k3l CD cord-320015-lbr2q4qh 206 3 and and CC cord-320015-lbr2q4qh 206 4 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 206 5 differ differ VBP cord-320015-lbr2q4qh 206 6 markedly markedly RB cord-320015-lbr2q4qh 206 7 in in IN cord-320015-lbr2q4qh 206 8 size size NN cord-320015-lbr2q4qh 206 9 , , , cord-320015-lbr2q4qh 206 10 they -PRON- PRP cord-320015-lbr2q4qh 206 11 share share VBP cord-320015-lbr2q4qh 206 12 a a DT cord-320015-lbr2q4qh 206 13 common common JJ cord-320015-lbr2q4qh 206 14 sequence sequence NN cord-320015-lbr2q4qh 206 15 motif motif NN cord-320015-lbr2q4qh 206 16 ( ( -LRB- cord-320015-lbr2q4qh 206 17 VDRVDREKGYVDL VDRVDREKGYVDL NNP cord-320015-lbr2q4qh 206 18 ) ) -RRB- cord-320015-lbr2q4qh 206 19 that that WDT cord-320015-lbr2q4qh 206 20 is be VBZ cord-320015-lbr2q4qh 206 21 likely likely RB cord-320015-lbr2q4qh 206 22 required require VBN cord-320015-lbr2q4qh 206 23 for for IN cord-320015-lbr2q4qh 206 24 activity activity NN cord-320015-lbr2q4qh 206 25 . . . cord-320015-lbr2q4qh 207 1 In in IN cord-320015-lbr2q4qh 207 2 this this DT cord-320015-lbr2q4qh 207 3 scenario scenario NN cord-320015-lbr2q4qh 207 4 , , , cord-320015-lbr2q4qh 207 5 PKR PKR NNP cord-320015-lbr2q4qh 207 6 binds bind VBZ cord-320015-lbr2q4qh 207 7 K3L k3l CD cord-320015-lbr2q4qh 207 8 / / SYM cord-320015-lbr2q4qh 207 9 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 207 10 instead instead RB cord-320015-lbr2q4qh 207 11 of of IN cord-320015-lbr2q4qh 207 12 eIF-2α eIF-2α NNS cord-320015-lbr2q4qh 207 13 , , , cord-320015-lbr2q4qh 207 14 and and CC cord-320015-lbr2q4qh 207 15 as as IN cord-320015-lbr2q4qh 207 16 a a DT cord-320015-lbr2q4qh 207 17 consequence consequence NN cord-320015-lbr2q4qh 207 18 eIF-2α eif-2α NN cord-320015-lbr2q4qh 207 19 is be VBZ cord-320015-lbr2q4qh 207 20 not not RB cord-320015-lbr2q4qh 207 21 phosphorylated phosphorylated JJ cord-320015-lbr2q4qh 207 22 and and CC cord-320015-lbr2q4qh 207 23 protein protein NN cord-320015-lbr2q4qh 207 24 synthesis synthesis NN cord-320015-lbr2q4qh 207 25 is be VBZ cord-320015-lbr2q4qh 207 26 maintained maintain VBN cord-320015-lbr2q4qh 207 27 . . . cord-320015-lbr2q4qh 208 1 To to TO cord-320015-lbr2q4qh 208 2 determine determine VB cord-320015-lbr2q4qh 208 3 if if IN cord-320015-lbr2q4qh 208 4 ATV ATV NNP cord-320015-lbr2q4qh 208 5 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 208 6 ( ( -LRB- cord-320015-lbr2q4qh 208 7 ORF ORF NNP cord-320015-lbr2q4qh 208 8 57R 57r CD cord-320015-lbr2q4qh 208 9 ) ) -RRB- cord-320015-lbr2q4qh 208 10 is be VBZ cord-320015-lbr2q4qh 208 11 functionally functionally RB cord-320015-lbr2q4qh 208 12 similar similar JJ cord-320015-lbr2q4qh 208 13 to to IN cord-320015-lbr2q4qh 208 14 K3L k3l CD cord-320015-lbr2q4qh 208 15 , , , cord-320015-lbr2q4qh 208 16 the the DT cord-320015-lbr2q4qh 208 17 ranavirus ranavirus NNP cord-320015-lbr2q4qh 208 18 gene gene NN cord-320015-lbr2q4qh 208 19 was be VBD cord-320015-lbr2q4qh 208 20 replaced replace VBN cord-320015-lbr2q4qh 208 21 with with IN cord-320015-lbr2q4qh 208 22 a a DT cord-320015-lbr2q4qh 208 23 selectable selectable JJ cord-320015-lbr2q4qh 208 24 marker marker NN cord-320015-lbr2q4qh 208 25 , , , cord-320015-lbr2q4qh 208 26 the the DT cord-320015-lbr2q4qh 208 27 neomycin neomycin NN cord-320015-lbr2q4qh 208 28 resistance resistance NN cord-320015-lbr2q4qh 208 29 ( ( -LRB- cord-320015-lbr2q4qh 208 30 NeoR NeoR NNP cord-320015-lbr2q4qh 208 31 ) ) -RRB- cord-320015-lbr2q4qh 208 32 gene gene NN cord-320015-lbr2q4qh 208 33 . . . cord-320015-lbr2q4qh 209 1 To to TO cord-320015-lbr2q4qh 209 2 generate generate VB cord-320015-lbr2q4qh 209 3 an an DT cord-320015-lbr2q4qh 209 4 ATV ATV NNP cord-320015-lbr2q4qh 209 5 KO KO NNP cord-320015-lbr2q4qh 209 6 mutant mutant NN cord-320015-lbr2q4qh 209 7 , , , cord-320015-lbr2q4qh 209 8 NeoR NeoR NNP cord-320015-lbr2q4qh 209 9 was be VBD cord-320015-lbr2q4qh 209 10 inserted insert VBN cord-320015-lbr2q4qh 209 11 downstream downstream RB cord-320015-lbr2q4qh 209 12 of of IN cord-320015-lbr2q4qh 209 13 the the DT cord-320015-lbr2q4qh 209 14 ATV ATV NNP cord-320015-lbr2q4qh 209 15 18 18 CD cord-320015-lbr2q4qh 209 16 K K NNP cord-320015-lbr2q4qh 209 17 IE IE NNP cord-320015-lbr2q4qh 209 18 promoter promoter NN cord-320015-lbr2q4qh 209 19 and and CC cord-320015-lbr2q4qh 209 20 this this DT cord-320015-lbr2q4qh 209 21 construct construct NN cord-320015-lbr2q4qh 209 22 was be VBD cord-320015-lbr2q4qh 209 23 bracketed bracket VBN cord-320015-lbr2q4qh 209 24 with with IN cord-320015-lbr2q4qh 209 25 sequences sequence NNS cord-320015-lbr2q4qh 209 26 identical identical JJ cord-320015-lbr2q4qh 209 27 to to IN cord-320015-lbr2q4qh 209 28 those those DT cord-320015-lbr2q4qh 209 29 in in IN cord-320015-lbr2q4qh 209 30 the the DT cord-320015-lbr2q4qh 209 31 flanking flank VBG cord-320015-lbr2q4qh 209 32 region region NN cord-320015-lbr2q4qh 209 33 of of IN cord-320015-lbr2q4qh 209 34 the the DT cord-320015-lbr2q4qh 209 35 targeted target VBN cord-320015-lbr2q4qh 209 36 gene gene NN cord-320015-lbr2q4qh 209 37 . . . cord-320015-lbr2q4qh 210 1 A a DT cord-320015-lbr2q4qh 210 2 PCR PCR NNP cord-320015-lbr2q4qh 210 3 product product NN cord-320015-lbr2q4qh 210 4 bearing bear VBG cord-320015-lbr2q4qh 210 5 this this DT cord-320015-lbr2q4qh 210 6 construct construct NN cord-320015-lbr2q4qh 210 7 was be VBD cord-320015-lbr2q4qh 210 8 then then RB cord-320015-lbr2q4qh 210 9 transfected transfecte VBN cord-320015-lbr2q4qh 210 10 into into IN cord-320015-lbr2q4qh 210 11 BF-2 BF-2 NNP cord-320015-lbr2q4qh 210 12 cells cell NNS cord-320015-lbr2q4qh 210 13 and and CC cord-320015-lbr2q4qh 210 14 infected infect VBN cord-320015-lbr2q4qh 210 15 with with IN cord-320015-lbr2q4qh 210 16 wild wild JJ cord-320015-lbr2q4qh 210 17 - - HYPH cord-320015-lbr2q4qh 210 18 type type NN cord-320015-lbr2q4qh 210 19 ( ( -LRB- cord-320015-lbr2q4qh 210 20 wt wt NN cord-320015-lbr2q4qh 210 21 ) ) -RRB- cord-320015-lbr2q4qh 210 22 ATV ATV NNP cord-320015-lbr2q4qh 210 23 . . . cord-320015-lbr2q4qh 211 1 Recombinant recombinant JJ cord-320015-lbr2q4qh 211 2 virus virus NN cord-320015-lbr2q4qh 211 3 was be VBD cord-320015-lbr2q4qh 211 4 isolated isolate VBN cord-320015-lbr2q4qh 211 5 by by IN cord-320015-lbr2q4qh 211 6 selective selective JJ cord-320015-lbr2q4qh 211 7 growth growth NN cord-320015-lbr2q4qh 211 8 in in IN cord-320015-lbr2q4qh 211 9 the the DT cord-320015-lbr2q4qh 211 10 presence presence NN cord-320015-lbr2q4qh 211 11 of of IN cord-320015-lbr2q4qh 211 12 neomycin neomycin NN cord-320015-lbr2q4qh 211 13 , , , cord-320015-lbr2q4qh 211 14 and and CC cord-320015-lbr2q4qh 211 15 replication replication NN cord-320015-lbr2q4qh 211 16 of of IN cord-320015-lbr2q4qh 211 17 the the DT cord-320015-lbr2q4qh 211 18 KO KO NNP cord-320015-lbr2q4qh 211 19 mutant mutant NN cord-320015-lbr2q4qh 211 20 was be VBD cord-320015-lbr2q4qh 211 21 compared compare VBN cord-320015-lbr2q4qh 211 22 to to TO cord-320015-lbr2q4qh 211 23 wt wt NN cord-320015-lbr2q4qh 211 24 virus virus NN cord-320015-lbr2q4qh 211 25 in in IN cord-320015-lbr2q4qh 211 26 vitro vitro FW cord-320015-lbr2q4qh 211 27 and and CC cord-320015-lbr2q4qh 211 28 in in IN cord-320015-lbr2q4qh 211 29 vivo vivo NNP cord-320015-lbr2q4qh 211 30 . . . cord-320015-lbr2q4qh 212 1 The the DT cord-320015-lbr2q4qh 212 2 KO KO NNP cord-320015-lbr2q4qh 212 3 mutant mutant NN cord-320015-lbr2q4qh 212 4 , , , cord-320015-lbr2q4qh 212 5 designated designate VBN cord-320015-lbr2q4qh 212 6 ATV∆57R ATV∆57R NNP cord-320015-lbr2q4qh 212 7 , , , cord-320015-lbr2q4qh 212 8 replicated replicate VBN cord-320015-lbr2q4qh 212 9 to to IN cord-320015-lbr2q4qh 212 10 titers titer NNS cord-320015-lbr2q4qh 212 11 comparable comparable JJ cord-320015-lbr2q4qh 212 12 to to IN cord-320015-lbr2q4qh 212 13 that that DT cord-320015-lbr2q4qh 212 14 of of IN cord-320015-lbr2q4qh 212 15 wt wt NN cord-320015-lbr2q4qh 212 16 ATV ATV NNP cord-320015-lbr2q4qh 212 17 in in IN cord-320015-lbr2q4qh 212 18 vitro vitro FW cord-320015-lbr2q4qh 212 19 , , , cord-320015-lbr2q4qh 212 20 but but CC cord-320015-lbr2q4qh 212 21 had have VBD cord-320015-lbr2q4qh 212 22 a a DT cord-320015-lbr2q4qh 212 23 small small JJ cord-320015-lbr2q4qh 212 24 plaque plaque NN cord-320015-lbr2q4qh 212 25 phenotype phenotype NN cord-320015-lbr2q4qh 212 26 . . . cord-320015-lbr2q4qh 213 1 In in IN cord-320015-lbr2q4qh 213 2 addition addition NN cord-320015-lbr2q4qh 213 3 , , , cord-320015-lbr2q4qh 213 4 ATV∆57R ATV∆57R NNP cord-320015-lbr2q4qh 213 5 was be VBD cord-320015-lbr2q4qh 213 6 8-fold 8-fold CD cord-320015-lbr2q4qh 213 7 more more RBR cord-320015-lbr2q4qh 213 8 sensitive sensitive JJ cord-320015-lbr2q4qh 213 9 to to IN cord-320015-lbr2q4qh 213 10 IFN IFN NNP cord-320015-lbr2q4qh 213 11 than than IN cord-320015-lbr2q4qh 213 12 wt wt NN cord-320015-lbr2q4qh 213 13 ATV ATV NNP cord-320015-lbr2q4qh 213 14 . . . cord-320015-lbr2q4qh 214 1 The the DT cord-320015-lbr2q4qh 214 2 increased increase VBN cord-320015-lbr2q4qh 214 3 IFN IFN NNP cord-320015-lbr2q4qh 214 4 sensitivity sensitivity NN cord-320015-lbr2q4qh 214 5 of of IN cord-320015-lbr2q4qh 214 6 ATV∆57R ATV∆57R NNP cord-320015-lbr2q4qh 214 7 was be VBD cord-320015-lbr2q4qh 214 8 correlated correlate VBN cord-320015-lbr2q4qh 214 9 with with IN cord-320015-lbr2q4qh 214 10 the the DT cord-320015-lbr2q4qh 214 11 increased increase VBN cord-320015-lbr2q4qh 214 12 phosphorylation phosphorylation NN cord-320015-lbr2q4qh 214 13 of of IN cord-320015-lbr2q4qh 214 14 eIF-2α eIF-2α NNS cord-320015-lbr2q4qh 214 15 and and CC cord-320015-lbr2q4qh 214 16 the the DT cord-320015-lbr2q4qh 214 17 lack lack NN cord-320015-lbr2q4qh 214 18 of of IN cord-320015-lbr2q4qh 214 19 PKR PKR NNP cord-320015-lbr2q4qh 214 20 degradation degradation NN cord-320015-lbr2q4qh 214 21 . . . cord-320015-lbr2q4qh 215 1 In in IN cord-320015-lbr2q4qh 215 2 contrast contrast NN cord-320015-lbr2q4qh 215 3 , , , cord-320015-lbr2q4qh 215 4 wt wt NN cord-320015-lbr2q4qh 215 5 ATV ATV NNP cord-320015-lbr2q4qh 215 6 degraded degrade VBD cord-320015-lbr2q4qh 215 7 PKR PKR NNP cord-320015-lbr2q4qh 215 8 and and CC cord-320015-lbr2q4qh 215 9 inhibited inhibit VBN cord-320015-lbr2q4qh 215 10 cellular cellular JJ cord-320015-lbr2q4qh 215 11 eIF-2α eif-2α NN cord-320015-lbr2q4qh 215 12 phosphorylation phosphorylation NN cord-320015-lbr2q4qh 215 13 . . . cord-320015-lbr2q4qh 216 1 In in IN cord-320015-lbr2q4qh 216 2 addition addition NN cord-320015-lbr2q4qh 216 3 , , , cord-320015-lbr2q4qh 216 4 ATV∆57R ATV∆57R NNP cord-320015-lbr2q4qh 216 5 was be VBD cord-320015-lbr2q4qh 216 6 less less RBR cord-320015-lbr2q4qh 216 7 pathogenic pathogenic JJ cord-320015-lbr2q4qh 216 8 than than IN cord-320015-lbr2q4qh 216 9 wt wt NN cord-320015-lbr2q4qh 216 10 ATV ATV NNP cord-320015-lbr2q4qh 217 1 following follow VBG cord-320015-lbr2q4qh 217 2 infection infection NN cord-320015-lbr2q4qh 217 3 of of IN cord-320015-lbr2q4qh 217 4 tiger tiger NN cord-320015-lbr2q4qh 217 5 salamanders salamander NNS cord-320015-lbr2q4qh 217 6 ( ( -LRB- cord-320015-lbr2q4qh 217 7 Ambystoma Ambystoma NNP cord-320015-lbr2q4qh 217 8 tigrinum tigrinum NNP cord-320015-lbr2q4qh 217 9 ) ) -RRB- cord-320015-lbr2q4qh 217 10 indicating indicate VBG cord-320015-lbr2q4qh 217 11 that that IN cord-320015-lbr2q4qh 217 12 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 217 13 was be VBD cord-320015-lbr2q4qh 217 14 a a DT cord-320015-lbr2q4qh 217 15 likely likely JJ cord-320015-lbr2q4qh 217 16 viral viral JJ cord-320015-lbr2q4qh 217 17 virulence virulence NN cord-320015-lbr2q4qh 217 18 / / SYM cord-320015-lbr2q4qh 217 19 immune immune JJ cord-320015-lbr2q4qh 217 20 evasion evasion NN cord-320015-lbr2q4qh 217 21 protein protein NN cord-320015-lbr2q4qh 217 22 . . . cord-320015-lbr2q4qh 218 1 Thus thus RB cord-320015-lbr2q4qh 218 2 the the DT cord-320015-lbr2q4qh 218 3 ATV ATV NNP cord-320015-lbr2q4qh 218 4 vIF-2α vif-2α NN cord-320015-lbr2q4qh 218 5 gene gene NN cord-320015-lbr2q4qh 218 6 is be VBZ cord-320015-lbr2q4qh 218 7 a a DT cord-320015-lbr2q4qh 218 8 putative putative JJ cord-320015-lbr2q4qh 218 9 IFN IFN NNP cord-320015-lbr2q4qh 218 10 - - HYPH cord-320015-lbr2q4qh 218 11 resistance resistance NN cord-320015-lbr2q4qh 218 12 gene gene NN cord-320015-lbr2q4qh 218 13 that that WDT cord-320015-lbr2q4qh 218 14 inhibits inhibit VBZ cord-320015-lbr2q4qh 218 15 cellular cellular JJ cord-320015-lbr2q4qh 218 16 innate innate JJ cord-320015-lbr2q4qh 218 17 immune immune JJ cord-320015-lbr2q4qh 218 18 responses response NNS cord-320015-lbr2q4qh 218 19 by by IN cord-320015-lbr2q4qh 218 20 degrading degrade VBG cord-320015-lbr2q4qh 218 21 PKR PKR NNP cord-320015-lbr2q4qh 218 22 and and CC cord-320015-lbr2q4qh 218 23 maintaining maintain VBG cord-320015-lbr2q4qh 218 24 high high JJ cord-320015-lbr2q4qh 218 25 levels level NNS cord-320015-lbr2q4qh 218 26 of of IN cord-320015-lbr2q4qh 218 27 viral viral JJ cord-320015-lbr2q4qh 218 28 protein protein NN cord-320015-lbr2q4qh 218 29 synthesis synthesis NN cord-320015-lbr2q4qh 218 30 . . . cord-320015-lbr2q4qh 219 1 In in IN cord-320015-lbr2q4qh 219 2 an an DT cord-320015-lbr2q4qh 219 3 effort effort NN cord-320015-lbr2q4qh 219 4 to to TO cord-320015-lbr2q4qh 219 5 improve improve VB cord-320015-lbr2q4qh 219 6 the the DT cord-320015-lbr2q4qh 219 7 KO KO NNP cord-320015-lbr2q4qh 219 8 methodology methodology NN cord-320015-lbr2q4qh 219 9 , , , cord-320015-lbr2q4qh 219 10 Chen Chen NNP cord-320015-lbr2q4qh 219 11 et et NNP cord-320015-lbr2q4qh 219 12 al al NNP cord-320015-lbr2q4qh 219 13 . . . cord-320015-lbr2q4qh 220 1 [ [ -LRB- cord-320015-lbr2q4qh 220 2 135 135 CD cord-320015-lbr2q4qh 220 3 ] ] -RRB- cord-320015-lbr2q4qh 220 4 recently recently RB cord-320015-lbr2q4qh 220 5 developed develop VBD cord-320015-lbr2q4qh 220 6 a a DT cord-320015-lbr2q4qh 220 7 potentially potentially RB cord-320015-lbr2q4qh 220 8 more more RBR cord-320015-lbr2q4qh 220 9 powerful powerful JJ cord-320015-lbr2q4qh 220 10 dual dual JJ cord-320015-lbr2q4qh 220 11 selection selection NN cord-320015-lbr2q4qh 220 12 method method NN cord-320015-lbr2q4qh 220 13 to to TO cord-320015-lbr2q4qh 220 14 generate generate VB cord-320015-lbr2q4qh 220 15 KO KO NNP cord-320015-lbr2q4qh 220 16 mutants mutant NNS cord-320015-lbr2q4qh 220 17 within within IN cord-320015-lbr2q4qh 220 18 FV3 FV3 NNP cord-320015-lbr2q4qh 220 19 . . . cord-320015-lbr2q4qh 221 1 In in IN cord-320015-lbr2q4qh 221 2 this this DT cord-320015-lbr2q4qh 221 3 system system NN cord-320015-lbr2q4qh 221 4 , , , cord-320015-lbr2q4qh 221 5 the the DT cord-320015-lbr2q4qh 221 6 gene gene NN cord-320015-lbr2q4qh 221 7 of of IN cord-320015-lbr2q4qh 221 8 interest interest NN cord-320015-lbr2q4qh 221 9 is be VBZ cord-320015-lbr2q4qh 221 10 replaced replace VBN cord-320015-lbr2q4qh 221 11 , , , cord-320015-lbr2q4qh 221 12 via via IN cord-320015-lbr2q4qh 221 13 homologous homologous JJ cord-320015-lbr2q4qh 221 14 recombination recombination NN cord-320015-lbr2q4qh 221 15 , , , cord-320015-lbr2q4qh 221 16 with with IN cord-320015-lbr2q4qh 221 17 a a DT cord-320015-lbr2q4qh 221 18 gene gene NN cord-320015-lbr2q4qh 221 19 encoding encode VBG cord-320015-lbr2q4qh 221 20 the the DT cord-320015-lbr2q4qh 221 21 puromycin puromycin NN cord-320015-lbr2q4qh 221 22 - - HYPH cord-320015-lbr2q4qh 221 23 resistance resistance NN cord-320015-lbr2q4qh 221 24 gene gene NN cord-320015-lbr2q4qh 221 25 fused fuse VBN cord-320015-lbr2q4qh 221 26 to to IN cord-320015-lbr2q4qh 221 27 the the DT cord-320015-lbr2q4qh 221 28 gene gene NN cord-320015-lbr2q4qh 221 29 for for IN cord-320015-lbr2q4qh 221 30 Enhanced Enhanced NNP cord-320015-lbr2q4qh 221 31 Green Green NNP cord-320015-lbr2q4qh 221 32 Fluorescent Fluorescent NNP cord-320015-lbr2q4qh 221 33 Protein Protein NNP cord-320015-lbr2q4qh 221 34 ( ( -LRB- cord-320015-lbr2q4qh 221 35 EGFP EGFP NNP cord-320015-lbr2q4qh 221 36 ) ) -RRB- cord-320015-lbr2q4qh 221 37 . . . cord-320015-lbr2q4qh 222 1 In in IN cord-320015-lbr2q4qh 222 2 initial initial JJ cord-320015-lbr2q4qh 222 3 experiments experiment NNS cord-320015-lbr2q4qh 222 4 KO KO NNP cord-320015-lbr2q4qh 222 5 mutants mutant NNS cord-320015-lbr2q4qh 222 6 targeting target VBG cord-320015-lbr2q4qh 222 7 the the DT cord-320015-lbr2q4qh 222 8 truncated truncated JJ cord-320015-lbr2q4qh 222 9 vIF-2α vif-2α JJ cord-320015-lbr2q4qh 222 10 protein protein NN cord-320015-lbr2q4qh 222 11 ( ( -LRB- cord-320015-lbr2q4qh 222 12 FV3-∆vIF-2α FV3-∆vIF-2α NNP cord-320015-lbr2q4qh 222 13 ) ) -RRB- cord-320015-lbr2q4qh 222 14 and and CC cord-320015-lbr2q4qh 222 15 the the DT cord-320015-lbr2q4qh 222 16 18 18 CD cord-320015-lbr2q4qh 222 17 K K NNP cord-320015-lbr2q4qh 222 18 IE IE NNP cord-320015-lbr2q4qh 222 19 protein protein NN cord-320015-lbr2q4qh 222 20 ( ( -LRB- cord-320015-lbr2q4qh 222 21 FV3-∆18 FV3-∆18 NNP cord-320015-lbr2q4qh 222 22 K K NNP cord-320015-lbr2q4qh 222 23 ) ) -RRB- cord-320015-lbr2q4qh 222 24 were be VBD cord-320015-lbr2q4qh 222 25 generated generate VBN cord-320015-lbr2q4qh 222 26 as as RB cord-320015-lbr2q4qh 222 27 well well RB cord-320015-lbr2q4qh 222 28 as as IN cord-320015-lbr2q4qh 222 29 a a DT cord-320015-lbr2q4qh 222 30 control control NN cord-320015-lbr2q4qh 222 31 , , , cord-320015-lbr2q4qh 222 32 " " '' cord-320015-lbr2q4qh 222 33 knock knock VB cord-320015-lbr2q4qh 222 34 in in RP cord-320015-lbr2q4qh 222 35 " " `` cord-320015-lbr2q4qh 222 36 mutant mutant JJ cord-320015-lbr2q4qh 222 37 in in IN cord-320015-lbr2q4qh 222 38 which which WDT cord-320015-lbr2q4qh 222 39 the the DT cord-320015-lbr2q4qh 222 40 puromycin puromycin NN cord-320015-lbr2q4qh 222 41 - - HYPH cord-320015-lbr2q4qh 222 42 resistance resistance NN cord-320015-lbr2q4qh 222 43 / / SYM cord-320015-lbr2q4qh 222 44 EGFP EGFP NNP cord-320015-lbr2q4qh 222 45 gene gene NN cord-320015-lbr2q4qh 222 46 was be VBD cord-320015-lbr2q4qh 222 47 inserted insert VBN cord-320015-lbr2q4qh 222 48 into into IN cord-320015-lbr2q4qh 222 49 a a DT cord-320015-lbr2q4qh 222 50 non non JJ cord-320015-lbr2q4qh 222 51 - - JJ cord-320015-lbr2q4qh 222 52 coding coding JJ cord-320015-lbr2q4qh 222 53 portion portion NN cord-320015-lbr2q4qh 222 54 of of IN cord-320015-lbr2q4qh 222 55 the the DT cord-320015-lbr2q4qh 222 56 genome genome NN cord-320015-lbr2q4qh 222 57 . . . cord-320015-lbr2q4qh 223 1 While while IN cord-320015-lbr2q4qh 223 2 all all DT cord-320015-lbr2q4qh 223 3 three three CD cord-320015-lbr2q4qh 223 4 recombinant recombinant JJ cord-320015-lbr2q4qh 223 5 viruses virus NNS cord-320015-lbr2q4qh 223 6 grew grow VBD cord-320015-lbr2q4qh 223 7 well well RB cord-320015-lbr2q4qh 223 8 in in IN cord-320015-lbr2q4qh 223 9 vitro vitro FW cord-320015-lbr2q4qh 223 10 , , , cord-320015-lbr2q4qh 223 11 the the DT cord-320015-lbr2q4qh 223 12 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 223 13 and and CC cord-320015-lbr2q4qh 223 14 18 18 CD cord-320015-lbr2q4qh 223 15 K K NNP cord-320015-lbr2q4qh 223 16 KO KO NNP cord-320015-lbr2q4qh 223 17 mutants mutant NNS cord-320015-lbr2q4qh 223 18 , , , cord-320015-lbr2q4qh 223 19 but but CC cord-320015-lbr2q4qh 223 20 not not RB cord-320015-lbr2q4qh 223 21 the the DT cord-320015-lbr2q4qh 223 22 control control NN cord-320015-lbr2q4qh 223 23 " " `` cord-320015-lbr2q4qh 223 24 knock knock VB cord-320015-lbr2q4qh 223 25 in in IN cord-320015-lbr2q4qh 223 26 " " `` cord-320015-lbr2q4qh 223 27 mutant mutant JJ cord-320015-lbr2q4qh 223 28 , , , cord-320015-lbr2q4qh 223 29 showed show VBD cord-320015-lbr2q4qh 223 30 a a DT cord-320015-lbr2q4qh 223 31 90 90 CD cord-320015-lbr2q4qh 223 32 % % NN cord-320015-lbr2q4qh 223 33 drop drop NN cord-320015-lbr2q4qh 223 34 in in IN cord-320015-lbr2q4qh 223 35 virus virus NN cord-320015-lbr2q4qh 223 36 levels level NNS cord-320015-lbr2q4qh 223 37 in in IN cord-320015-lbr2q4qh 223 38 X. X. NNP cord-320015-lbr2q4qh 223 39 laevis laevi NNS cord-320015-lbr2q4qh 223 40 tadpoles tadpole NNS cord-320015-lbr2q4qh 223 41 . . . cord-320015-lbr2q4qh 224 1 These these DT cord-320015-lbr2q4qh 224 2 results result NNS cord-320015-lbr2q4qh 224 3 confirmed confirm VBD cord-320015-lbr2q4qh 224 4 the the DT cord-320015-lbr2q4qh 224 5 previous previous JJ cord-320015-lbr2q4qh 224 6 observation observation NN cord-320015-lbr2q4qh 224 7 that that IN cord-320015-lbr2q4qh 224 8 the the DT cord-320015-lbr2q4qh 224 9 18 18 CD cord-320015-lbr2q4qh 224 10 K K NNP cord-320015-lbr2q4qh 224 11 protein protein NN cord-320015-lbr2q4qh 224 12 was be VBD cord-320015-lbr2q4qh 224 13 not not RB cord-320015-lbr2q4qh 224 14 required require VBN cord-320015-lbr2q4qh 224 15 for for IN cord-320015-lbr2q4qh 224 16 replication replication NN cord-320015-lbr2q4qh 224 17 in in IN cord-320015-lbr2q4qh 224 18 vitro vitro FW cord-320015-lbr2q4qh 224 19 [ [ -LRB- cord-320015-lbr2q4qh 224 20 52 52 CD cord-320015-lbr2q4qh 224 21 ] ] -RRB- cord-320015-lbr2q4qh 224 22 , , , cord-320015-lbr2q4qh 224 23 and and CC cord-320015-lbr2q4qh 224 24 indicate indicate VBP cord-320015-lbr2q4qh 224 25 that that IN cord-320015-lbr2q4qh 224 26 18 18 CD cord-320015-lbr2q4qh 224 27 K K NNP cord-320015-lbr2q4qh 224 28 plays play VBZ cord-320015-lbr2q4qh 224 29 a a DT cord-320015-lbr2q4qh 224 30 role role NN cord-320015-lbr2q4qh 224 31 , , , cord-320015-lbr2q4qh 224 32 albeit albeit IN cord-320015-lbr2q4qh 224 33 unknown unknown JJ cord-320015-lbr2q4qh 224 34 , , , cord-320015-lbr2q4qh 224 35 in in IN cord-320015-lbr2q4qh 224 36 replication replication NN cord-320015-lbr2q4qh 224 37 in in IN cord-320015-lbr2q4qh 224 38 vivo vivo NNP cord-320015-lbr2q4qh 224 39 . . . cord-320015-lbr2q4qh 225 1 The the DT cord-320015-lbr2q4qh 225 2 finding finding NN cord-320015-lbr2q4qh 225 3 that that IN cord-320015-lbr2q4qh 225 4 the the DT cord-320015-lbr2q4qh 225 5 truncated truncated JJ cord-320015-lbr2q4qh 225 6 vIF-2α vif-2α JJ cord-320015-lbr2q4qh 225 7 protein protein NN cord-320015-lbr2q4qh 225 8 of of IN cord-320015-lbr2q4qh 225 9 FV3 FV3 NNP cord-320015-lbr2q4qh 225 10 was be VBD cord-320015-lbr2q4qh 225 11 also also RB cord-320015-lbr2q4qh 225 12 required require VBN cord-320015-lbr2q4qh 225 13 for for IN cord-320015-lbr2q4qh 225 14 replication replication NN cord-320015-lbr2q4qh 225 15 in in IN cord-320015-lbr2q4qh 225 16 vivo vivo NNP cord-320015-lbr2q4qh 225 17 was be VBD cord-320015-lbr2q4qh 225 18 surprising surprising JJ cord-320015-lbr2q4qh 225 19 because because IN cord-320015-lbr2q4qh 225 20 the the DT cord-320015-lbr2q4qh 225 21 FV3 FV3 NNP cord-320015-lbr2q4qh 225 22 homolog homolog NN cord-320015-lbr2q4qh 225 23 of of IN cord-320015-lbr2q4qh 225 24 vIF-2α vIF-2α NNPS cord-320015-lbr2q4qh 225 25 is be VBZ cord-320015-lbr2q4qh 225 26 missing miss VBG cord-320015-lbr2q4qh 225 27 the the DT cord-320015-lbr2q4qh 225 28 N n CD cord-320015-lbr2q4qh 225 29 - - HYPH cord-320015-lbr2q4qh 225 30 terminal terminal JJ cord-320015-lbr2q4qh 225 31 two two CD cord-320015-lbr2q4qh 225 32 - - HYPH cord-320015-lbr2q4qh 225 33 thirds third NNS cord-320015-lbr2q4qh 225 34 of of IN cord-320015-lbr2q4qh 225 35 this this DT cord-320015-lbr2q4qh 225 36 protein protein NN cord-320015-lbr2q4qh 225 37 , , , cord-320015-lbr2q4qh 225 38 including include VBG cord-320015-lbr2q4qh 225 39 the the DT cord-320015-lbr2q4qh 225 40 region region NN cord-320015-lbr2q4qh 225 41 homologous homologous JJ cord-320015-lbr2q4qh 225 42 to to IN cord-320015-lbr2q4qh 225 43 vaccinia vaccinia NN cord-320015-lbr2q4qh 225 44 virus virus NN cord-320015-lbr2q4qh 226 1 K3L k3l JJ cord-320015-lbr2q4qh 226 2 and and CC cord-320015-lbr2q4qh 226 3 eukaryotic eukaryotic JJ cord-320015-lbr2q4qh 226 4 translational translational JJ cord-320015-lbr2q4qh 226 5 initiation initiation NN cord-320015-lbr2q4qh 226 6 factor factor NN cord-320015-lbr2q4qh 226 7 2α 2α CD cord-320015-lbr2q4qh 226 8 . . . cord-320015-lbr2q4qh 227 1 Inspection inspection NN cord-320015-lbr2q4qh 227 2 of of IN cord-320015-lbr2q4qh 227 3 the the DT cord-320015-lbr2q4qh 227 4 FV3 FV3 NNP cord-320015-lbr2q4qh 227 5 nucleotide nucleotide JJ cord-320015-lbr2q4qh 227 6 sequence sequence NN cord-320015-lbr2q4qh 227 7 indicated indicate VBD cord-320015-lbr2q4qh 227 8 that that IN cord-320015-lbr2q4qh 227 9 FV3 FV3 NNP cord-320015-lbr2q4qh 227 10 vIF-2α vIF-2α NNP cord-320015-lbr2q4qh 227 11 is be VBZ cord-320015-lbr2q4qh 227 12 a a DT cord-320015-lbr2q4qh 227 13 chimeric chimeric JJ cord-320015-lbr2q4qh 227 14 protein protein NN cord-320015-lbr2q4qh 227 15 resulting result VBG cord-320015-lbr2q4qh 227 16 from from IN cord-320015-lbr2q4qh 227 17 deletion deletion NN cord-320015-lbr2q4qh 227 18 and and CC cord-320015-lbr2q4qh 227 19 in in IN cord-320015-lbr2q4qh 227 20 - - HYPH cord-320015-lbr2q4qh 227 21 frame frame NN cord-320015-lbr2q4qh 227 22 fusion fusion NN cord-320015-lbr2q4qh 227 23 of of IN cord-320015-lbr2q4qh 227 24 a a DT cord-320015-lbr2q4qh 227 25 small small JJ cord-320015-lbr2q4qh 227 26 upstream upstream JJ cord-320015-lbr2q4qh 227 27 ORF ORF NNP cord-320015-lbr2q4qh 227 28 with with IN cord-320015-lbr2q4qh 227 29 the the DT cord-320015-lbr2q4qh 227 30 larger large JJR cord-320015-lbr2q4qh 227 31 , , , cord-320015-lbr2q4qh 227 32 downstream downstream JJ cord-320015-lbr2q4qh 227 33 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 227 34 ORF ORF NNP cord-320015-lbr2q4qh 227 35 . . . cord-320015-lbr2q4qh 228 1 The the DT cord-320015-lbr2q4qh 228 2 resulting result VBG cord-320015-lbr2q4qh 228 3 product product NN cord-320015-lbr2q4qh 228 4 contains contain VBZ cord-320015-lbr2q4qh 228 5 10 10 CD cord-320015-lbr2q4qh 228 6 amino amino NN cord-320015-lbr2q4qh 228 7 acids acid NNS cord-320015-lbr2q4qh 228 8 from from IN cord-320015-lbr2q4qh 228 9 the the DT cord-320015-lbr2q4qh 228 10 N n CD cord-320015-lbr2q4qh 228 11 - - HYPH cord-320015-lbr2q4qh 228 12 terminus terminus NN cord-320015-lbr2q4qh 228 13 of of IN cord-320015-lbr2q4qh 228 14 the the DT cord-320015-lbr2q4qh 228 15 upstream upstream JJ cord-320015-lbr2q4qh 228 16 ORF ORF NNP cord-320015-lbr2q4qh 228 17 and and CC cord-320015-lbr2q4qh 228 18 the the DT cord-320015-lbr2q4qh 228 19 last last JJ cord-320015-lbr2q4qh 228 20 65 65 CD cord-320015-lbr2q4qh 228 21 amino amino JJ cord-320015-lbr2q4qh 228 22 acids acid NNS cord-320015-lbr2q4qh 228 23 of of IN cord-320015-lbr2q4qh 228 24 the the DT cord-320015-lbr2q4qh 228 25 full full JJ cord-320015-lbr2q4qh 228 26 - - HYPH cord-320015-lbr2q4qh 228 27 length length NN cord-320015-lbr2q4qh 228 28 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 228 29 protein protein NN cord-320015-lbr2q4qh 228 30 . . . cord-320015-lbr2q4qh 229 1 The the DT cord-320015-lbr2q4qh 229 2 reduced reduce VBN cord-320015-lbr2q4qh 229 3 replication replication NN cord-320015-lbr2q4qh 229 4 of of IN cord-320015-lbr2q4qh 229 5 FV3-∆vIF-2α fv3-∆vif-2α NN cord-320015-lbr2q4qh 229 6 in in IN cord-320015-lbr2q4qh 229 7 vivo vivo NNP cord-320015-lbr2q4qh 229 8 suggests suggest VBZ cord-320015-lbr2q4qh 229 9 that that IN cord-320015-lbr2q4qh 229 10 the the DT cord-320015-lbr2q4qh 229 11 C C NNP cord-320015-lbr2q4qh 229 12 - - HYPH cord-320015-lbr2q4qh 229 13 terminal terminal JJ cord-320015-lbr2q4qh 229 14 end end NN cord-320015-lbr2q4qh 229 15 of of IN cord-320015-lbr2q4qh 229 16 the the DT cord-320015-lbr2q4qh 229 17 FV3 FV3 NNP cord-320015-lbr2q4qh 229 18 vIF-2α vif-2α JJ cord-320015-lbr2q4qh 229 19 homolog homolog NN cord-320015-lbr2q4qh 229 20 is be VBZ cord-320015-lbr2q4qh 229 21 required require VBN cord-320015-lbr2q4qh 229 22 for for IN cord-320015-lbr2q4qh 229 23 full full JJ cord-320015-lbr2q4qh 229 24 replication replication NN cord-320015-lbr2q4qh 229 25 in in IN cord-320015-lbr2q4qh 229 26 vivo vivo NN cord-320015-lbr2q4qh 229 27 . . . cord-320015-lbr2q4qh 230 1 In in IN cord-320015-lbr2q4qh 230 2 addition addition NN cord-320015-lbr2q4qh 230 3 to to IN cord-320015-lbr2q4qh 230 4 the the DT cord-320015-lbr2q4qh 230 5 above above JJ cord-320015-lbr2q4qh 230 6 KO KO NNP cord-320015-lbr2q4qh 230 7 mutants mutant NNS cord-320015-lbr2q4qh 230 8 , , , cord-320015-lbr2q4qh 230 9 a a DT cord-320015-lbr2q4qh 230 10 recombinant recombinant JJ cord-320015-lbr2q4qh 230 11 soft soft RB cord-320015-lbr2q4qh 230 12 - - HYPH cord-320015-lbr2q4qh 230 13 shelled shell VBN cord-320015-lbr2q4qh 230 14 turtle turtle NN cord-320015-lbr2q4qh 230 15 iridovirus iridovirus NN cord-320015-lbr2q4qh 230 16 has have VBZ cord-320015-lbr2q4qh 230 17 recently recently RB cord-320015-lbr2q4qh 230 18 been be VBN cord-320015-lbr2q4qh 230 19 constructed construct VBN cord-320015-lbr2q4qh 230 20 that that WDT cord-320015-lbr2q4qh 230 21 expresses express VBZ cord-320015-lbr2q4qh 230 22 EGFP EGFP NNP cord-320015-lbr2q4qh 230 23 . . . cord-320015-lbr2q4qh 231 1 EGFP EGFP NNP cord-320015-lbr2q4qh 231 2 - - HYPH cord-320015-lbr2q4qh 231 3 expressing express VBG cord-320015-lbr2q4qh 231 4 mutants mutant NNS cord-320015-lbr2q4qh 231 5 may may MD cord-320015-lbr2q4qh 231 6 provide provide VB cord-320015-lbr2q4qh 231 7 an an DT cord-320015-lbr2q4qh 231 8 alternative alternative NN cord-320015-lbr2q4qh 231 9 , , , cord-320015-lbr2q4qh 231 10 simplified simplified JJ cord-320015-lbr2q4qh 231 11 strategy strategy NN cord-320015-lbr2q4qh 231 12 for for IN cord-320015-lbr2q4qh 231 13 screening screen VBG cord-320015-lbr2q4qh 231 14 antiviral antiviral JJ cord-320015-lbr2q4qh 231 15 substrates substrate NNS cord-320015-lbr2q4qh 231 16 through through IN cord-320015-lbr2q4qh 231 17 the the DT cord-320015-lbr2q4qh 231 18 visualization visualization NN cord-320015-lbr2q4qh 231 19 and and CC cord-320015-lbr2q4qh 231 20 quantification quantification NN cord-320015-lbr2q4qh 231 21 of of IN cord-320015-lbr2q4qh 231 22 fluorescent fluorescent JJ cord-320015-lbr2q4qh 231 23 virus virus NN cord-320015-lbr2q4qh 231 24 [ [ -LRB- cord-320015-lbr2q4qh 231 25 136 136 CD cord-320015-lbr2q4qh 231 26 ] ] -RRB- cord-320015-lbr2q4qh 231 27 . . . cord-320015-lbr2q4qh 232 1 In in IN cord-320015-lbr2q4qh 232 2 contrast contrast NN cord-320015-lbr2q4qh 232 3 to to IN cord-320015-lbr2q4qh 232 4 the the DT cord-320015-lbr2q4qh 232 5 above above JJ cord-320015-lbr2q4qh 232 6 studies study NNS cord-320015-lbr2q4qh 232 7 , , , cord-320015-lbr2q4qh 232 8 a a DT cord-320015-lbr2q4qh 232 9 fourth fourth JJ cord-320015-lbr2q4qh 232 10 approach approach NN cord-320015-lbr2q4qh 232 11 for for IN cord-320015-lbr2q4qh 232 12 determining determine VBG cord-320015-lbr2q4qh 232 13 viral viral JJ cord-320015-lbr2q4qh 232 14 gene gene NN cord-320015-lbr2q4qh 232 15 function function NN cord-320015-lbr2q4qh 232 16 involves involve VBZ cord-320015-lbr2q4qh 232 17 the the DT cord-320015-lbr2q4qh 232 18 synthesis synthesis NN cord-320015-lbr2q4qh 232 19 of of IN cord-320015-lbr2q4qh 232 20 recombinant recombinant JJ cord-320015-lbr2q4qh 232 21 viral viral JJ cord-320015-lbr2q4qh 232 22 proteins protein NNS cord-320015-lbr2q4qh 232 23 and and CC cord-320015-lbr2q4qh 232 24 assessment assessment NN cord-320015-lbr2q4qh 232 25 of of IN cord-320015-lbr2q4qh 232 26 their -PRON- PRP$ cord-320015-lbr2q4qh 232 27 functions function NNS cord-320015-lbr2q4qh 232 28 in in IN cord-320015-lbr2q4qh 232 29 vivo vivo NN cord-320015-lbr2q4qh 232 30 and and CC cord-320015-lbr2q4qh 232 31 in in IN cord-320015-lbr2q4qh 232 32 vitro vitro FW cord-320015-lbr2q4qh 232 33 . . . cord-320015-lbr2q4qh 233 1 Several several JJ cord-320015-lbr2q4qh 233 2 investigators investigator NNS cord-320015-lbr2q4qh 233 3 have have VBP cord-320015-lbr2q4qh 233 4 used use VBN cord-320015-lbr2q4qh 233 5 this this DT cord-320015-lbr2q4qh 233 6 approach approach NN cord-320015-lbr2q4qh 233 7 and and CC cord-320015-lbr2q4qh 233 8 representative representative JJ cord-320015-lbr2q4qh 233 9 examples example NNS cord-320015-lbr2q4qh 233 10 are be VBP cord-320015-lbr2q4qh 233 11 discussed discuss VBN cord-320015-lbr2q4qh 233 12 below below RB cord-320015-lbr2q4qh 233 13 . . . cord-320015-lbr2q4qh 234 1 A a DT cord-320015-lbr2q4qh 234 2 putative putative JJ cord-320015-lbr2q4qh 234 3 homolog homolog NN cord-320015-lbr2q4qh 234 4 of of IN cord-320015-lbr2q4qh 234 5 FV3 FV3 NNP cord-320015-lbr2q4qh 234 6 ICP46 ICP46 NNP cord-320015-lbr2q4qh 234 7 was be VBD cord-320015-lbr2q4qh 234 8 identified identify VBN cord-320015-lbr2q4qh 234 9 in in IN cord-320015-lbr2q4qh 234 10 SGIV SGIV NNP cord-320015-lbr2q4qh 234 11 [ [ -LRB- cord-320015-lbr2q4qh 234 12 137 137 CD cord-320015-lbr2q4qh 234 13 ] ] -RRB- cord-320015-lbr2q4qh 234 14 . . . cord-320015-lbr2q4qh 235 1 ICP46 icp46 RB cord-320015-lbr2q4qh 236 1 ( ( -LRB- cord-320015-lbr2q4qh 236 2 mol mol NN cord-320015-lbr2q4qh 236 3 wt wt NN cord-320015-lbr2q4qh 236 4 44.1 44.1 CD cord-320015-lbr2q4qh 236 5 kDa kDa NNPS cord-320015-lbr2q4qh 236 6 ) ) -RRB- cord-320015-lbr2q4qh 236 7 is be VBZ cord-320015-lbr2q4qh 236 8 an an DT cord-320015-lbr2q4qh 236 9 IE IE NNP cord-320015-lbr2q4qh 236 10 gene gene NN cord-320015-lbr2q4qh 236 11 product product NN cord-320015-lbr2q4qh 236 12 that that WDT cord-320015-lbr2q4qh 236 13 is be VBZ cord-320015-lbr2q4qh 236 14 distributed distribute VBN cord-320015-lbr2q4qh 236 15 predominantly predominantly RB cord-320015-lbr2q4qh 236 16 within within IN cord-320015-lbr2q4qh 236 17 the the DT cord-320015-lbr2q4qh 236 18 cytoplasm cytoplasm NN cord-320015-lbr2q4qh 236 19 but but CC cord-320015-lbr2q4qh 236 20 is be VBZ cord-320015-lbr2q4qh 236 21 also also RB cord-320015-lbr2q4qh 236 22 found find VBN cord-320015-lbr2q4qh 236 23 within within IN cord-320015-lbr2q4qh 236 24 virions virion NNS cord-320015-lbr2q4qh 236 25 . . . cord-320015-lbr2q4qh 237 1 A a DT cord-320015-lbr2q4qh 237 2 plasmid plasmid NN cord-320015-lbr2q4qh 237 3 expressing express VBG cord-320015-lbr2q4qh 237 4 ICP46 icp46 NN cord-320015-lbr2q4qh 237 5 was be VBD cord-320015-lbr2q4qh 237 6 introduced introduce VBN cord-320015-lbr2q4qh 237 7 into into IN cord-320015-lbr2q4qh 237 8 grouper grouper NN cord-320015-lbr2q4qh 237 9 embryonic embryonic JJ cord-320015-lbr2q4qh 237 10 ( ( -LRB- cord-320015-lbr2q4qh 237 11 GP GP NNP cord-320015-lbr2q4qh 237 12 ) ) -RRB- cord-320015-lbr2q4qh 237 13 and and CC cord-320015-lbr2q4qh 237 14 fathead fathead NN cord-320015-lbr2q4qh 237 15 minnow minnow NN cord-320015-lbr2q4qh 237 16 cells cell NNS cord-320015-lbr2q4qh 237 17 and and CC cord-320015-lbr2q4qh 237 18 stably stably RB cord-320015-lbr2q4qh 237 19 transfected transfecte VBN cord-320015-lbr2q4qh 237 20 cells cell NNS cord-320015-lbr2q4qh 237 21 were be VBD cord-320015-lbr2q4qh 237 22 characterized characterize VBN cord-320015-lbr2q4qh 237 23 . . . cord-320015-lbr2q4qh 238 1 Over over IN cord-320015-lbr2q4qh 238 2 expression expression NN cord-320015-lbr2q4qh 238 3 of of IN cord-320015-lbr2q4qh 238 4 SGIV SGIV NNP cord-320015-lbr2q4qh 238 5 ICP46 ICP46 NNP cord-320015-lbr2q4qh 238 6 resulted result VBD cord-320015-lbr2q4qh 238 7 in in IN cord-320015-lbr2q4qh 238 8 higher high JJR cord-320015-lbr2q4qh 238 9 cell cell NN cord-320015-lbr2q4qh 238 10 densities density NNS cord-320015-lbr2q4qh 238 11 and and CC cord-320015-lbr2q4qh 238 12 increased increase VBN cord-320015-lbr2q4qh 238 13 growth growth NN cord-320015-lbr2q4qh 238 14 of of IN cord-320015-lbr2q4qh 238 15 monolayer monolayer NN cord-320015-lbr2q4qh 238 16 cultures culture NNS cord-320015-lbr2q4qh 238 17 . . . cord-320015-lbr2q4qh 239 1 SGIV SGIV NNP cord-320015-lbr2q4qh 239 2 replication replication NN cord-320015-lbr2q4qh 239 3 was be VBD cord-320015-lbr2q4qh 239 4 compared compare VBN cord-320015-lbr2q4qh 239 5 in in IN cord-320015-lbr2q4qh 239 6 GP gp NN cord-320015-lbr2q4qh 239 7 cells cell NNS cord-320015-lbr2q4qh 239 8 transfected transfecte VBN cord-320015-lbr2q4qh 239 9 with with IN cord-320015-lbr2q4qh 239 10 an an DT cord-320015-lbr2q4qh 239 11 empty empty JJ cord-320015-lbr2q4qh 239 12 vector vector NN cord-320015-lbr2q4qh 239 13 and and CC cord-320015-lbr2q4qh 239 14 in in IN cord-320015-lbr2q4qh 239 15 GP GP NNP cord-320015-lbr2q4qh 239 16 cells cell NNS cord-320015-lbr2q4qh 239 17 expressing express VBG cord-320015-lbr2q4qh 239 18 ICP46 icp46 RB cord-320015-lbr2q4qh 239 19 . . . cord-320015-lbr2q4qh 240 1 SGIV SGIV NNP cord-320015-lbr2q4qh 240 2 replicated replicate VBD cord-320015-lbr2q4qh 240 3 more more RBR cord-320015-lbr2q4qh 240 4 rapidly rapidly RB cord-320015-lbr2q4qh 240 5 and and CC cord-320015-lbr2q4qh 240 6 reached reach VBN cord-320015-lbr2q4qh 240 7 titers titer NNS cord-320015-lbr2q4qh 240 8 that that WDT cord-320015-lbr2q4qh 240 9 were be VBD cord-320015-lbr2q4qh 240 10 10-fold 10-fold JJ cord-320015-lbr2q4qh 240 11 higher high JJR cord-320015-lbr2q4qh 240 12 in in IN cord-320015-lbr2q4qh 240 13 ICP46 icp46 NN cord-320015-lbr2q4qh 240 14 expressing express VBG cord-320015-lbr2q4qh 240 15 cells cell NNS cord-320015-lbr2q4qh 240 16 than than IN cord-320015-lbr2q4qh 240 17 in in IN cord-320015-lbr2q4qh 240 18 control control NN cord-320015-lbr2q4qh 240 19 cells cell NNS cord-320015-lbr2q4qh 240 20 . . . cord-320015-lbr2q4qh 241 1 Although although IN cord-320015-lbr2q4qh 241 2 conserved conserved JJ cord-320015-lbr2q4qh 241 3 domains domain NNS cord-320015-lbr2q4qh 241 4 suggestive suggestive JJ cord-320015-lbr2q4qh 241 5 of of IN cord-320015-lbr2q4qh 241 6 function function NN cord-320015-lbr2q4qh 241 7 were be VBD cord-320015-lbr2q4qh 241 8 not not RB cord-320015-lbr2q4qh 241 9 detected detect VBN cord-320015-lbr2q4qh 241 10 within within IN cord-320015-lbr2q4qh 241 11 ICP46 ICP46 NNP cord-320015-lbr2q4qh 241 12 , , , cord-320015-lbr2q4qh 241 13 the the DT cord-320015-lbr2q4qh 241 14 authors author NNS cord-320015-lbr2q4qh 241 15 speculate speculate VBP cord-320015-lbr2q4qh 241 16 , , , cord-320015-lbr2q4qh 241 17 based base VBN cord-320015-lbr2q4qh 241 18 on on IN cord-320015-lbr2q4qh 241 19 the the DT cord-320015-lbr2q4qh 241 20 above above JJ cord-320015-lbr2q4qh 241 21 results result NNS cord-320015-lbr2q4qh 241 22 , , , cord-320015-lbr2q4qh 241 23 that that DT cord-320015-lbr2q4qh 241 24 ICP46 icp46 NN cord-320015-lbr2q4qh 241 25 might may MD cord-320015-lbr2q4qh 241 26 encode encode VB cord-320015-lbr2q4qh 241 27 a a DT cord-320015-lbr2q4qh 241 28 protein protein NN cord-320015-lbr2q4qh 241 29 involved involve VBN cord-320015-lbr2q4qh 241 30 in in IN cord-320015-lbr2q4qh 241 31 cell cell NN cord-320015-lbr2q4qh 241 32 growth growth NN cord-320015-lbr2q4qh 241 33 control control NN cord-320015-lbr2q4qh 241 34 . . . cord-320015-lbr2q4qh 242 1 The the DT cord-320015-lbr2q4qh 242 2 authors author NNS cord-320015-lbr2q4qh 242 3 suggested suggest VBD cord-320015-lbr2q4qh 242 4 that that IN cord-320015-lbr2q4qh 242 5 ICP46 icp46 RB cord-320015-lbr2q4qh 242 6 , , , cord-320015-lbr2q4qh 242 7 like like IN cord-320015-lbr2q4qh 242 8 IE IE NNP cord-320015-lbr2q4qh 242 9 proteins protein NNS cord-320015-lbr2q4qh 242 10 in in IN cord-320015-lbr2q4qh 242 11 other other JJ cord-320015-lbr2q4qh 242 12 viral viral JJ cord-320015-lbr2q4qh 242 13 systems system NNS cord-320015-lbr2q4qh 242 14 , , , cord-320015-lbr2q4qh 242 15 might may MD cord-320015-lbr2q4qh 242 16 control control VB cord-320015-lbr2q4qh 242 17 host host NN cord-320015-lbr2q4qh 242 18 cell cell NN cord-320015-lbr2q4qh 242 19 growth growth NN cord-320015-lbr2q4qh 242 20 by by IN cord-320015-lbr2q4qh 242 21 regulating regulate VBG cord-320015-lbr2q4qh 242 22 the the DT cord-320015-lbr2q4qh 242 23 cell cell NN cord-320015-lbr2q4qh 242 24 cycle cycle NN cord-320015-lbr2q4qh 242 25 , , , cord-320015-lbr2q4qh 242 26 preventing prevent VBG cord-320015-lbr2q4qh 242 27 growth growth NN cord-320015-lbr2q4qh 242 28 arrest arrest NN cord-320015-lbr2q4qh 242 29 , , , cord-320015-lbr2q4qh 242 30 or or CC cord-320015-lbr2q4qh 242 31 delaying delay VBG cord-320015-lbr2q4qh 242 32 apoptosis apoptosis NN cord-320015-lbr2q4qh 242 33 and and CC cord-320015-lbr2q4qh 242 34 play play VB cord-320015-lbr2q4qh 242 35 a a DT cord-320015-lbr2q4qh 242 36 critical critical JJ cord-320015-lbr2q4qh 242 37 role role NN cord-320015-lbr2q4qh 242 38 in in IN cord-320015-lbr2q4qh 242 39 promoting promote VBG cord-320015-lbr2q4qh 242 40 a a DT cord-320015-lbr2q4qh 242 41 proliferative proliferative JJ cord-320015-lbr2q4qh 242 42 environment environment NN cord-320015-lbr2q4qh 242 43 that that WDT cord-320015-lbr2q4qh 242 44 enhances enhance VBZ cord-320015-lbr2q4qh 242 45 virus virus NN cord-320015-lbr2q4qh 242 46 replication replication NN cord-320015-lbr2q4qh 242 47 . . . cord-320015-lbr2q4qh 243 1 ISKNV ISKNV NNP cord-320015-lbr2q4qh 243 2 ( ( -LRB- cord-320015-lbr2q4qh 243 3 genus genus NN cord-320015-lbr2q4qh 244 1 Megalocytivirus Megalocytivirus NNP cord-320015-lbr2q4qh 244 2 ) ) -RRB- cord-320015-lbr2q4qh 244 3 ORF ORF NNP cord-320015-lbr2q4qh 244 4 48R 48r LS cord-320015-lbr2q4qh 244 5 encodes encode VBZ cord-320015-lbr2q4qh 244 6 a a DT cord-320015-lbr2q4qh 244 7 viral viral JJ cord-320015-lbr2q4qh 244 8 protein protein NN cord-320015-lbr2q4qh 244 9 with with IN cord-320015-lbr2q4qh 244 10 marked marked JJ cord-320015-lbr2q4qh 244 11 similarity similarity NN cord-320015-lbr2q4qh 244 12 to to IN cord-320015-lbr2q4qh 244 13 vascular vascular JJ cord-320015-lbr2q4qh 244 14 endothelial endothelial JJ cord-320015-lbr2q4qh 244 15 growth growth NN cord-320015-lbr2q4qh 244 16 factor factor NN cord-320015-lbr2q4qh 244 17 ( ( -LRB- cord-320015-lbr2q4qh 244 18 vVEGF vvegf NN cord-320015-lbr2q4qh 244 19 ) ) -RRB- cord-320015-lbr2q4qh 245 1 [ [ -LRB- cord-320015-lbr2q4qh 245 2 138 138 CD cord-320015-lbr2q4qh 245 3 ] ] -RRB- cord-320015-lbr2q4qh 245 4 . . . cord-320015-lbr2q4qh 246 1 Microinjection microinjection NN cord-320015-lbr2q4qh 246 2 of of IN cord-320015-lbr2q4qh 246 3 a a DT cord-320015-lbr2q4qh 246 4 plasmid plasmid NN cord-320015-lbr2q4qh 246 5 expressing express VBG cord-320015-lbr2q4qh 246 6 vVEGF vvegf NN cord-320015-lbr2q4qh 246 7 into into IN cord-320015-lbr2q4qh 246 8 zebrafish zebrafish NN cord-320015-lbr2q4qh 246 9 one one CD cord-320015-lbr2q4qh 246 10 - - HYPH cord-320015-lbr2q4qh 246 11 cell cell NN cord-320015-lbr2q4qh 246 12 embryos embryo NNS cord-320015-lbr2q4qh 246 13 resulted result VBD cord-320015-lbr2q4qh 246 14 in in IN cord-320015-lbr2q4qh 246 15 pericardial pericardial JJ cord-320015-lbr2q4qh 246 16 edema edema NN cord-320015-lbr2q4qh 246 17 and and CC cord-320015-lbr2q4qh 246 18 dilation dilation NN cord-320015-lbr2q4qh 246 19 of of IN cord-320015-lbr2q4qh 246 20 the the DT cord-320015-lbr2q4qh 246 21 tail tail NN cord-320015-lbr2q4qh 246 22 region region NN cord-320015-lbr2q4qh 246 23 suggesting suggest VBG cord-320015-lbr2q4qh 246 24 that that IN cord-320015-lbr2q4qh 246 25 vVEGF vvegf NN cord-320015-lbr2q4qh 246 26 triggers trigger VBZ cord-320015-lbr2q4qh 246 27 vascular vascular JJ cord-320015-lbr2q4qh 246 28 permeability permeability NN cord-320015-lbr2q4qh 246 29 . . . cord-320015-lbr2q4qh 247 1 Moreover moreover RB cord-320015-lbr2q4qh 247 2 , , , cord-320015-lbr2q4qh 247 3 vVEGF vvegf NN cord-320015-lbr2q4qh 247 4 binds bind NNS cord-320015-lbr2q4qh 247 5 and and CC cord-320015-lbr2q4qh 247 6 up up RB cord-320015-lbr2q4qh 247 7 - - HYPH cord-320015-lbr2q4qh 247 8 regulates regulate VBZ cord-320015-lbr2q4qh 247 9 the the DT cord-320015-lbr2q4qh 247 10 expression expression NN cord-320015-lbr2q4qh 247 11 of of IN cord-320015-lbr2q4qh 247 12 FLK-1 FLK-1 NNP cord-320015-lbr2q4qh 247 13 and and CC cord-320015-lbr2q4qh 247 14 together together RB cord-320015-lbr2q4qh 247 15 both both DT cord-320015-lbr2q4qh 247 16 proteins protein NNS cord-320015-lbr2q4qh 247 17 likely likely RB cord-320015-lbr2q4qh 247 18 contribute contribute VBP cord-320015-lbr2q4qh 247 19 to to IN cord-320015-lbr2q4qh 247 20 the the DT cord-320015-lbr2q4qh 247 21 proliferative proliferative JJ cord-320015-lbr2q4qh 247 22 and and CC cord-320015-lbr2q4qh 247 23 highly highly RB cord-320015-lbr2q4qh 247 24 vascularized vascularized JJ cord-320015-lbr2q4qh 247 25 nature nature NN cord-320015-lbr2q4qh 247 26 of of IN cord-320015-lbr2q4qh 247 27 ISKNV ISKNV NNP cord-320015-lbr2q4qh 247 28 lesions lesion NNS cord-320015-lbr2q4qh 247 29 in in IN cord-320015-lbr2q4qh 247 30 its -PRON- PRP$ cord-320015-lbr2q4qh 247 31 natural natural JJ cord-320015-lbr2q4qh 247 32 host host NN cord-320015-lbr2q4qh 247 33 , , , cord-320015-lbr2q4qh 248 1 the the DT cord-320015-lbr2q4qh 248 2 Chinese chinese JJ cord-320015-lbr2q4qh 248 3 mandarin mandarin NN cord-320015-lbr2q4qh 248 4 fish fish NN cord-320015-lbr2q4qh 248 5 . . . cord-320015-lbr2q4qh 249 1 Although although IN cord-320015-lbr2q4qh 249 2 these these DT cord-320015-lbr2q4qh 249 3 results result NNS cord-320015-lbr2q4qh 249 4 suggest suggest VBP cord-320015-lbr2q4qh 249 5 that that IN cord-320015-lbr2q4qh 249 6 ISKNV ISKNV NNP cord-320015-lbr2q4qh 249 7 ORF48R orf48r NN cord-320015-lbr2q4qh 249 8 plays play VBZ cord-320015-lbr2q4qh 249 9 a a DT cord-320015-lbr2q4qh 249 10 vital vital JJ cord-320015-lbr2q4qh 249 11 role role NN cord-320015-lbr2q4qh 249 12 in in IN cord-320015-lbr2q4qh 249 13 host host NN cord-320015-lbr2q4qh 249 14 infection infection NN cord-320015-lbr2q4qh 249 15 and and CC cord-320015-lbr2q4qh 249 16 viral viral JJ cord-320015-lbr2q4qh 249 17 replication replication NN cord-320015-lbr2q4qh 249 18 , , , cord-320015-lbr2q4qh 249 19 its -PRON- PRP$ cord-320015-lbr2q4qh 249 20 precise precise JJ cord-320015-lbr2q4qh 249 21 function function NN cord-320015-lbr2q4qh 249 22 remains remain VBZ cord-320015-lbr2q4qh 249 23 unclear unclear JJ cord-320015-lbr2q4qh 249 24 . . . cord-320015-lbr2q4qh 250 1 Following follow VBG cord-320015-lbr2q4qh 250 2 Orf Orf NNP cord-320015-lbr2q4qh 250 3 virus virus NN cord-320015-lbr2q4qh 250 4 ( ( -LRB- cord-320015-lbr2q4qh 250 5 family family NN cord-320015-lbr2q4qh 250 6 Poxviridae Poxviridae NNP cord-320015-lbr2q4qh 250 7 ) ) -RRB- cord-320015-lbr2q4qh 250 8 infection infection NN cord-320015-lbr2q4qh 250 9 , , , cord-320015-lbr2q4qh 250 10 the the DT cord-320015-lbr2q4qh 250 11 vVEGF vvegf NN cord-320015-lbr2q4qh 250 12 homolog homolog NN cord-320015-lbr2q4qh 250 13 aids aids NNP cord-320015-lbr2q4qh 250 14 in in IN cord-320015-lbr2q4qh 250 15 scab scab NN cord-320015-lbr2q4qh 250 16 formation formation NN cord-320015-lbr2q4qh 250 17 and and CC cord-320015-lbr2q4qh 250 18 wound wound VB cord-320015-lbr2q4qh 250 19 healing healing NN cord-320015-lbr2q4qh 250 20 and and CC cord-320015-lbr2q4qh 250 21 may may MD cord-320015-lbr2q4qh 250 22 enhance enhance VB cord-320015-lbr2q4qh 250 23 transmission transmission NN cord-320015-lbr2q4qh 250 24 since since IN cord-320015-lbr2q4qh 250 25 scabs scab NNS cord-320015-lbr2q4qh 250 26 contains contain VBZ cord-320015-lbr2q4qh 250 27 substantial substantial JJ cord-320015-lbr2q4qh 250 28 amounts amount NNS cord-320015-lbr2q4qh 250 29 of of IN cord-320015-lbr2q4qh 250 30 infectious infectious JJ cord-320015-lbr2q4qh 250 31 virus virus NN cord-320015-lbr2q4qh 250 32 . . . cord-320015-lbr2q4qh 251 1 A a DT cord-320015-lbr2q4qh 251 2 novel novel JJ cord-320015-lbr2q4qh 251 3 histone histone NN cord-320015-lbr2q4qh 251 4 - - HYPH cord-320015-lbr2q4qh 251 5 binding bind VBG cord-320015-lbr2q4qh 251 6 protein protein NN cord-320015-lbr2q4qh 251 7 was be VBD cord-320015-lbr2q4qh 251 8 identified identify VBN cord-320015-lbr2q4qh 251 9 in in IN cord-320015-lbr2q4qh 251 10 SGIV SGIV NNP cord-320015-lbr2q4qh 251 11 by by IN cord-320015-lbr2q4qh 251 12 structural structural JJ cord-320015-lbr2q4qh 251 13 analysis analysis NN cord-320015-lbr2q4qh 251 14 of of IN cord-320015-lbr2q4qh 251 15 the the DT cord-320015-lbr2q4qh 251 16 ORF ORF NNP cord-320015-lbr2q4qh 251 17 158L 158l CD cord-320015-lbr2q4qh 251 18 gene gene NN cord-320015-lbr2q4qh 251 19 product product NN cord-320015-lbr2q4qh 251 20 [ [ -LRB- cord-320015-lbr2q4qh 251 21 139 139 CD cord-320015-lbr2q4qh 251 22 ] ] -RRB- cord-320015-lbr2q4qh 251 23 . . . cord-320015-lbr2q4qh 252 1 X x NN cord-320015-lbr2q4qh 252 2 - - NN cord-320015-lbr2q4qh 252 3 ray ray NN cord-320015-lbr2q4qh 252 4 diffraction diffraction NN cord-320015-lbr2q4qh 252 5 analysis analysis NN cord-320015-lbr2q4qh 252 6 of of IN cord-320015-lbr2q4qh 252 7 recombinant recombinant JJ cord-320015-lbr2q4qh 252 8 158L 158l CD cord-320015-lbr2q4qh 252 9 determined determine VBD cord-320015-lbr2q4qh 252 10 the the DT cord-320015-lbr2q4qh 252 11 crystal crystal JJ cord-320015-lbr2q4qh 252 12 structure structure NN cord-320015-lbr2q4qh 252 13 at at IN cord-320015-lbr2q4qh 252 14 a a DT cord-320015-lbr2q4qh 252 15 resolution resolution NN cord-320015-lbr2q4qh 252 16 of of IN cord-320015-lbr2q4qh 252 17 2.2 2.2 CD cord-320015-lbr2q4qh 252 18 Å å NN cord-320015-lbr2q4qh 252 19 , , , cord-320015-lbr2q4qh 252 20 and and CC cord-320015-lbr2q4qh 252 21 revealed reveal VBD cord-320015-lbr2q4qh 252 22 that that IN cord-320015-lbr2q4qh 252 23 158L 158l CD cord-320015-lbr2q4qh 252 24 exhibited exhibit VBD cord-320015-lbr2q4qh 252 25 partial partial JJ cord-320015-lbr2q4qh 252 26 structural structural JJ cord-320015-lbr2q4qh 252 27 resemblance resemblance NN cord-320015-lbr2q4qh 252 28 to to IN cord-320015-lbr2q4qh 252 29 the the DT cord-320015-lbr2q4qh 252 30 histone histone NN cord-320015-lbr2q4qh 252 31 - - HYPH cord-320015-lbr2q4qh 252 32 binding bind VBG cord-320015-lbr2q4qh 252 33 region region NN cord-320015-lbr2q4qh 252 34 of of IN cord-320015-lbr2q4qh 252 35 anti anti JJ cord-320015-lbr2q4qh 252 36 - - JJ cord-320015-lbr2q4qh 252 37 silencing silencing JJ cord-320015-lbr2q4qh 252 38 factor factor NN cord-320015-lbr2q4qh 252 39 1 1 CD cord-320015-lbr2q4qh 252 40 ( ( -LRB- cord-320015-lbr2q4qh 252 41 Asf1 Asf1 NNP cord-320015-lbr2q4qh 252 42 ) ) -RRB- cord-320015-lbr2q4qh 252 43 , , , cord-320015-lbr2q4qh 252 44 a a DT cord-320015-lbr2q4qh 252 45 histone histone NN cord-320015-lbr2q4qh 252 46 H3 H3 NNP cord-320015-lbr2q4qh 252 47 / / SYM cord-320015-lbr2q4qh 252 48 H4 H4 NNP cord-320015-lbr2q4qh 252 49 chaperon chaperon NN cord-320015-lbr2q4qh 252 50 . . . cord-320015-lbr2q4qh 253 1 Using use VBG cord-320015-lbr2q4qh 253 2 recombinant recombinant JJ cord-320015-lbr2q4qh 253 3 158L 158l CD cord-320015-lbr2q4qh 253 4 protein protein NN cord-320015-lbr2q4qh 253 5 , , , cord-320015-lbr2q4qh 253 6 interaction interaction NN cord-320015-lbr2q4qh 253 7 was be VBD cord-320015-lbr2q4qh 253 8 demonstrated demonstrate VBN cord-320015-lbr2q4qh 253 9 between between IN cord-320015-lbr2q4qh 253 10 158L 158l CD cord-320015-lbr2q4qh 253 11 and and CC cord-320015-lbr2q4qh 253 12 histone histone NN cord-320015-lbr2q4qh 253 13 H3 H3 NNP cord-320015-lbr2q4qh 253 14 / / SYM cord-320015-lbr2q4qh 253 15 H4 H4 NNP cord-320015-lbr2q4qh 253 16 complexes complex NNS cord-320015-lbr2q4qh 253 17 and and CC cord-320015-lbr2q4qh 253 18 H3 H3 NNP cord-320015-lbr2q4qh 253 19 by by IN cord-320015-lbr2q4qh 253 20 isothermal isothermal JJ cord-320015-lbr2q4qh 253 21 titration titration NN cord-320015-lbr2q4qh 253 22 calorimetry calorimetry NN cord-320015-lbr2q4qh 253 23 . . . cord-320015-lbr2q4qh 254 1 The the DT cord-320015-lbr2q4qh 254 2 authors author NNS cord-320015-lbr2q4qh 254 3 suggested suggest VBD cord-320015-lbr2q4qh 254 4 that that IN cord-320015-lbr2q4qh 254 5 158L 158l CD cord-320015-lbr2q4qh 254 6 may may MD cord-320015-lbr2q4qh 254 7 be be VB cord-320015-lbr2q4qh 254 8 involved involve VBN cord-320015-lbr2q4qh 254 9 in in IN cord-320015-lbr2q4qh 254 10 both both CC cord-320015-lbr2q4qh 254 11 the the DT cord-320015-lbr2q4qh 254 12 regulation regulation NN cord-320015-lbr2q4qh 254 13 and and CC cord-320015-lbr2q4qh 254 14 expression expression NN cord-320015-lbr2q4qh 254 15 of of IN cord-320015-lbr2q4qh 254 16 histone histone NN cord-320015-lbr2q4qh 254 17 H3 H3 NNP cord-320015-lbr2q4qh 254 18 and and CC cord-320015-lbr2q4qh 254 19 H4 H4 NNP cord-320015-lbr2q4qh 254 20 methylation methylation NN cord-320015-lbr2q4qh 254 21 , , , cord-320015-lbr2q4qh 254 22 events event NNS cord-320015-lbr2q4qh 254 23 which which WDT cord-320015-lbr2q4qh 254 24 allow allow VBP cord-320015-lbr2q4qh 254 25 the the DT cord-320015-lbr2q4qh 254 26 virus virus NN cord-320015-lbr2q4qh 254 27 to to TO cord-320015-lbr2q4qh 254 28 control control VB cord-320015-lbr2q4qh 254 29 host host NN cord-320015-lbr2q4qh 254 30 cell cell NN cord-320015-lbr2q4qh 254 31 gene gene NN cord-320015-lbr2q4qh 254 32 expression expression NN cord-320015-lbr2q4qh 254 33 and and CC cord-320015-lbr2q4qh 254 34 facilitate facilitate VB cord-320015-lbr2q4qh 254 35 viral viral JJ cord-320015-lbr2q4qh 254 36 replication replication NN cord-320015-lbr2q4qh 254 37 . . . cord-320015-lbr2q4qh 255 1 SGIV SGIV NNP cord-320015-lbr2q4qh 255 2 ORF ORF NNP cord-320015-lbr2q4qh 255 3 86R 86R NNP cord-320015-lbr2q4qh 255 4 encodes encode VBZ cord-320015-lbr2q4qh 255 5 a a DT cord-320015-lbr2q4qh 255 6 homolog homolog NN cord-320015-lbr2q4qh 255 7 of of IN cord-320015-lbr2q4qh 255 8 the the DT cord-320015-lbr2q4qh 255 9 FV3 FV3 NNP cord-320015-lbr2q4qh 255 10 18 18 CD cord-320015-lbr2q4qh 255 11 K K NNP cord-320015-lbr2q4qh 255 12 immediate immediate JJ cord-320015-lbr2q4qh 255 13 early early JJ cord-320015-lbr2q4qh 255 14 protein protein NN cord-320015-lbr2q4qh 255 15 . . . cord-320015-lbr2q4qh 256 1 Although although IN cord-320015-lbr2q4qh 256 2 KD KD NNP cord-320015-lbr2q4qh 256 3 studies study NNS cord-320015-lbr2q4qh 256 4 using use VBG cord-320015-lbr2q4qh 256 5 an an DT cord-320015-lbr2q4qh 256 6 asMO asmo NN cord-320015-lbr2q4qh 256 7 targeted target VBN cord-320015-lbr2q4qh 256 8 against against IN cord-320015-lbr2q4qh 256 9 18 18 CD cord-320015-lbr2q4qh 256 10 K K NNP cord-320015-lbr2q4qh 256 11 [ [ -LRB- cord-320015-lbr2q4qh 256 12 52 52 CD cord-320015-lbr2q4qh 256 13 ] ] -RRB- cord-320015-lbr2q4qh 256 14 and and CC cord-320015-lbr2q4qh 256 15 an an DT cord-320015-lbr2q4qh 256 16 18 18 CD cord-320015-lbr2q4qh 256 17 K K NNP cord-320015-lbr2q4qh 256 18 knock knock VB cord-320015-lbr2q4qh 256 19 out out RP cord-320015-lbr2q4qh 256 20 mutant mutant JJ cord-320015-lbr2q4qh 256 21 [ [ -LRB- cord-320015-lbr2q4qh 256 22 135 135 CD cord-320015-lbr2q4qh 256 23 ] ] -RRB- cord-320015-lbr2q4qh 256 24 indicated indicate VBD cord-320015-lbr2q4qh 256 25 that that DT cord-320015-lbr2q4qh 256 26 expression expression NN cord-320015-lbr2q4qh 256 27 of of IN cord-320015-lbr2q4qh 256 28 18 18 CD cord-320015-lbr2q4qh 256 29 K K NNP cord-320015-lbr2q4qh 256 30 was be VBD cord-320015-lbr2q4qh 256 31 not not RB cord-320015-lbr2q4qh 256 32 required require VBN cord-320015-lbr2q4qh 256 33 for for IN cord-320015-lbr2q4qh 256 34 replication replication NN cord-320015-lbr2q4qh 256 35 in in IN cord-320015-lbr2q4qh 256 36 either either CC cord-320015-lbr2q4qh 256 37 FHM FHM NNP cord-320015-lbr2q4qh 256 38 or or CC cord-320015-lbr2q4qh 256 39 BHK BHK NNP cord-320015-lbr2q4qh 256 40 cells cell NNS cord-320015-lbr2q4qh 256 41 , , , cord-320015-lbr2q4qh 256 42 replication replication NN cord-320015-lbr2q4qh 256 43 of of IN cord-320015-lbr2q4qh 256 44 the the DT cord-320015-lbr2q4qh 256 45 18 18 CD cord-320015-lbr2q4qh 256 46 K K NNP cord-320015-lbr2q4qh 256 47 KO KO NNP cord-320015-lbr2q4qh 256 48 in in IN cord-320015-lbr2q4qh 256 49 Xenopus Xenopus NNP cord-320015-lbr2q4qh 256 50 laevis laevi NNS cord-320015-lbr2q4qh 256 51 tadpoles tadpole NNS cord-320015-lbr2q4qh 256 52 was be VBD cord-320015-lbr2q4qh 256 53 reduced reduce VBN cord-320015-lbr2q4qh 256 54 about about IN cord-320015-lbr2q4qh 256 55 10-fold 10-fold JJ cord-320015-lbr2q4qh 256 56 compared compare VBN cord-320015-lbr2q4qh 256 57 to to IN cord-320015-lbr2q4qh 256 58 wt wt NN cord-320015-lbr2q4qh 256 59 virus virus NN cord-320015-lbr2q4qh 256 60 or or CC cord-320015-lbr2q4qh 256 61 a a DT cord-320015-lbr2q4qh 256 62 " " `` cord-320015-lbr2q4qh 256 63 knock knock VB cord-320015-lbr2q4qh 256 64 in in IN cord-320015-lbr2q4qh 256 65 " " `` cord-320015-lbr2q4qh 256 66 mutant mutant JJ cord-320015-lbr2q4qh 256 67 . . . cord-320015-lbr2q4qh 257 1 To to TO cord-320015-lbr2q4qh 257 2 determine determine VB cord-320015-lbr2q4qh 257 3 the the DT cord-320015-lbr2q4qh 257 4 intracellular intracellular JJ cord-320015-lbr2q4qh 257 5 location location NN cord-320015-lbr2q4qh 257 6 of of IN cord-320015-lbr2q4qh 257 7 the the DT cord-320015-lbr2q4qh 257 8 18 18 CD cord-320015-lbr2q4qh 257 9 K K NNP cord-320015-lbr2q4qh 257 10 gene gene NN cord-320015-lbr2q4qh 257 11 product product NN cord-320015-lbr2q4qh 257 12 , , , cord-320015-lbr2q4qh 257 13 Xia Xia NNP cord-320015-lbr2q4qh 257 14 et et NNP cord-320015-lbr2q4qh 257 15 al al NNP cord-320015-lbr2q4qh 257 16 . . . cord-320015-lbr2q4qh 258 1 [ [ -LRB- cord-320015-lbr2q4qh 258 2 140 140 CD cord-320015-lbr2q4qh 258 3 ] ] -RRB- cord-320015-lbr2q4qh 258 4 generated generate VBN cord-320015-lbr2q4qh 258 5 recombinant recombinant JJ cord-320015-lbr2q4qh 258 6 SGIV SGIV NNP cord-320015-lbr2q4qh 258 7 18 18 CD cord-320015-lbr2q4qh 258 8 K K NNP cord-320015-lbr2q4qh 258 9 protein protein NN cord-320015-lbr2q4qh 258 10 and and CC cord-320015-lbr2q4qh 258 11 used use VBD cord-320015-lbr2q4qh 258 12 it -PRON- PRP cord-320015-lbr2q4qh 258 13 to to TO cord-320015-lbr2q4qh 258 14 produce produce VB cord-320015-lbr2q4qh 258 15 rabbit rabbit NN cord-320015-lbr2q4qh 258 16 anti-18 anti-18 NN cord-320015-lbr2q4qh 258 17 K K NNP cord-320015-lbr2q4qh 258 18 serum serum NN cord-320015-lbr2q4qh 258 19 . . . cord-320015-lbr2q4qh 259 1 Immunofluorescent Immunofluorescent NNP cord-320015-lbr2q4qh 259 2 assay assay NN cord-320015-lbr2q4qh 259 3 showed show VBD cord-320015-lbr2q4qh 259 4 SGIV SGIV NNP cord-320015-lbr2q4qh 259 5 18 18 CD cord-320015-lbr2q4qh 259 6 K K NNP cord-320015-lbr2q4qh 259 7 to to TO cord-320015-lbr2q4qh 259 8 be be VB cord-320015-lbr2q4qh 259 9 distributed distribute VBN cord-320015-lbr2q4qh 259 10 within within IN cord-320015-lbr2q4qh 259 11 the the DT cord-320015-lbr2q4qh 259 12 cytoplasm cytoplasm NN cord-320015-lbr2q4qh 259 13 adjacent adjacent JJ cord-320015-lbr2q4qh 259 14 to to IN cord-320015-lbr2q4qh 259 15 VAS VAS NNP cord-320015-lbr2q4qh 259 16 and and CC cord-320015-lbr2q4qh 259 17 nuclei nucleus NNS cord-320015-lbr2q4qh 259 18 , , , cord-320015-lbr2q4qh 259 19 and and CC cord-320015-lbr2q4qh 259 20 Western western JJ cord-320015-lbr2q4qh 259 21 blotting blotting NN cord-320015-lbr2q4qh 259 22 using use VBG cord-320015-lbr2q4qh 259 23 purified purify VBN cord-320015-lbr2q4qh 259 24 and and CC cord-320015-lbr2q4qh 259 25 disrupted disrupt VBN cord-320015-lbr2q4qh 259 26 virions virion NNS cord-320015-lbr2q4qh 259 27 indicated indicate VBD cord-320015-lbr2q4qh 259 28 18 18 CD cord-320015-lbr2q4qh 259 29 K K NNP cord-320015-lbr2q4qh 259 30 was be VBD cord-320015-lbr2q4qh 259 31 a a DT cord-320015-lbr2q4qh 259 32 non non JJ cord-320015-lbr2q4qh 259 33 - - JJ cord-320015-lbr2q4qh 259 34 envelope envelope JJ cord-320015-lbr2q4qh 259 35 virion virion NN cord-320015-lbr2q4qh 259 36 - - HYPH cord-320015-lbr2q4qh 259 37 associated associate VBN cord-320015-lbr2q4qh 259 38 protein protein NN cord-320015-lbr2q4qh 259 39 . . . cord-320015-lbr2q4qh 260 1 Transfection transfection NN cord-320015-lbr2q4qh 260 2 of of IN cord-320015-lbr2q4qh 260 3 uninfected uninfected JJ cord-320015-lbr2q4qh 260 4 grouper grouper NN cord-320015-lbr2q4qh 260 5 cells cell NNS cord-320015-lbr2q4qh 260 6 with with IN cord-320015-lbr2q4qh 260 7 a a DT cord-320015-lbr2q4qh 260 8 vector vector NN cord-320015-lbr2q4qh 260 9 expressing express VBG cord-320015-lbr2q4qh 260 10 18 18 CD cord-320015-lbr2q4qh 260 11 K K NNP cord-320015-lbr2q4qh 260 12 enhanced enhance VBN cord-320015-lbr2q4qh 260 13 cellular cellular JJ cord-320015-lbr2q4qh 260 14 growth growth NN cord-320015-lbr2q4qh 260 15 rates rate NNS cord-320015-lbr2q4qh 260 16 and and CC cord-320015-lbr2q4qh 260 17 led lead VBD cord-320015-lbr2q4qh 260 18 to to IN cord-320015-lbr2q4qh 260 19 an an DT cord-320015-lbr2q4qh 260 20 increase increase NN cord-320015-lbr2q4qh 260 21 in in IN cord-320015-lbr2q4qh 260 22 the the DT cord-320015-lbr2q4qh 260 23 density density NN cord-320015-lbr2q4qh 260 24 of of IN cord-320015-lbr2q4qh 260 25 monolayer monolayer NN cord-320015-lbr2q4qh 260 26 cultures culture NNS cord-320015-lbr2q4qh 260 27 . . . cord-320015-lbr2q4qh 261 1 Moreover moreover RB cord-320015-lbr2q4qh 261 2 , , , cord-320015-lbr2q4qh 261 3 SGIV sgiv JJ cord-320015-lbr2q4qh 261 4 replication replication NN cord-320015-lbr2q4qh 261 5 in in IN cord-320015-lbr2q4qh 261 6 cultures culture NNS cord-320015-lbr2q4qh 261 7 expressing express VBG cord-320015-lbr2q4qh 261 8 18 18 CD cord-320015-lbr2q4qh 261 9 K K NNP cord-320015-lbr2q4qh 261 10 were be VBD cord-320015-lbr2q4qh 261 11 about about RB cord-320015-lbr2q4qh 261 12 10-fold 10-fold JJ cord-320015-lbr2q4qh 261 13 higher high JJR cord-320015-lbr2q4qh 261 14 than than IN cord-320015-lbr2q4qh 261 15 in in IN cord-320015-lbr2q4qh 261 16 cultures culture NNS cord-320015-lbr2q4qh 261 17 transfected transfecte VBN cord-320015-lbr2q4qh 261 18 with with IN cord-320015-lbr2q4qh 261 19 an an DT cord-320015-lbr2q4qh 261 20 empty empty JJ cord-320015-lbr2q4qh 261 21 vector vector NN cord-320015-lbr2q4qh 261 22 . . . cord-320015-lbr2q4qh 262 1 The the DT cord-320015-lbr2q4qh 262 2 authors author NNS cord-320015-lbr2q4qh 262 3 suggest suggest VBP cord-320015-lbr2q4qh 262 4 that that IN cord-320015-lbr2q4qh 262 5 18 18 CD cord-320015-lbr2q4qh 262 6 K K NNP cord-320015-lbr2q4qh 262 7 may may MD cord-320015-lbr2q4qh 262 8 be be VB cord-320015-lbr2q4qh 262 9 a a DT cord-320015-lbr2q4qh 262 10 protein protein NN cord-320015-lbr2q4qh 262 11 that that WDT cord-320015-lbr2q4qh 262 12 plays play VBZ cord-320015-lbr2q4qh 262 13 a a DT cord-320015-lbr2q4qh 262 14 role role NN cord-320015-lbr2q4qh 262 15 in in IN cord-320015-lbr2q4qh 262 16 the the DT cord-320015-lbr2q4qh 262 17 control control NN cord-320015-lbr2q4qh 262 18 of of IN cord-320015-lbr2q4qh 262 19 cellular cellular JJ cord-320015-lbr2q4qh 262 20 growth growth NN cord-320015-lbr2q4qh 262 21 and and CC cord-320015-lbr2q4qh 262 22 thus thus RB cord-320015-lbr2q4qh 262 23 indirectly indirectly RB cord-320015-lbr2q4qh 262 24 enhances enhance VBZ cord-320015-lbr2q4qh 262 25 SGIV SGIV NNP cord-320015-lbr2q4qh 262 26 replication replication NN cord-320015-lbr2q4qh 262 27 . . . cord-320015-lbr2q4qh 263 1 However however RB cord-320015-lbr2q4qh 263 2 , , , cord-320015-lbr2q4qh 263 3 because because IN cord-320015-lbr2q4qh 263 4 virus virus NN cord-320015-lbr2q4qh 263 5 yields yield NNS cord-320015-lbr2q4qh 263 6 were be VBD cord-320015-lbr2q4qh 263 7 expressed express VBN cord-320015-lbr2q4qh 263 8 as as IN cord-320015-lbr2q4qh 263 9 TCID TCID NNP cord-320015-lbr2q4qh 263 10 50 50 CD cord-320015-lbr2q4qh 263 11 /mL /mL NFP cord-320015-lbr2q4qh 263 12 , , , cord-320015-lbr2q4qh 263 13 it -PRON- PRP cord-320015-lbr2q4qh 263 14 was be VBD cord-320015-lbr2q4qh 263 15 not not RB cord-320015-lbr2q4qh 263 16 clear clear JJ cord-320015-lbr2q4qh 263 17 if if IN cord-320015-lbr2q4qh 263 18 the the DT cord-320015-lbr2q4qh 263 19 increase increase NN cord-320015-lbr2q4qh 263 20 in in IN cord-320015-lbr2q4qh 263 21 SGIV SGIV NNP cord-320015-lbr2q4qh 263 22 yield yield NN cord-320015-lbr2q4qh 263 23 reflected reflect VBD cord-320015-lbr2q4qh 263 24 higher high JJR cord-320015-lbr2q4qh 263 25 virus virus NN cord-320015-lbr2q4qh 263 26 production production NN cord-320015-lbr2q4qh 263 27 per per IN cord-320015-lbr2q4qh 263 28 cell cell NN cord-320015-lbr2q4qh 263 29 or or CC cord-320015-lbr2q4qh 263 30 simply simply RB cord-320015-lbr2q4qh 263 31 equivalent equivalent JJ cord-320015-lbr2q4qh 263 32 cellular cellular JJ cord-320015-lbr2q4qh 263 33 yields yield NNS cord-320015-lbr2q4qh 263 34 in in IN cord-320015-lbr2q4qh 263 35 cultures culture NNS cord-320015-lbr2q4qh 263 36 containing contain VBG cord-320015-lbr2q4qh 263 37 varying vary VBG cord-320015-lbr2q4qh 263 38 numbers number NNS cord-320015-lbr2q4qh 263 39 of of IN cord-320015-lbr2q4qh 263 40 cells cell NNS cord-320015-lbr2q4qh 263 41 . . . cord-320015-lbr2q4qh 264 1 Rothenburg Rothenburg NNP cord-320015-lbr2q4qh 264 2 et et FW cord-320015-lbr2q4qh 264 3 al al NNP cord-320015-lbr2q4qh 264 4 . . . cord-320015-lbr2q4qh 265 1 [ [ -LRB- cord-320015-lbr2q4qh 265 2 61 61 CD cord-320015-lbr2q4qh 265 3 ] ] -RRB- cord-320015-lbr2q4qh 265 4 cloned clone VBD cord-320015-lbr2q4qh 265 5 the the DT cord-320015-lbr2q4qh 265 6 vIF-2α vif-2α JJ cord-320015-lbr2q4qh 265 7 gene gene NN cord-320015-lbr2q4qh 265 8 from from IN cord-320015-lbr2q4qh 265 9 Rana Rana NNP cord-320015-lbr2q4qh 265 10 catesbeiana catesbeiana NNP cord-320015-lbr2q4qh 265 11 virus virus NN cord-320015-lbr2q4qh 265 12 Z z NN cord-320015-lbr2q4qh 265 13 ( ( -LRB- cord-320015-lbr2q4qh 265 14 RCV RCV NNP cord-320015-lbr2q4qh 265 15 - - HYPH cord-320015-lbr2q4qh 265 16 Z Z NNP cord-320015-lbr2q4qh 265 17 ) ) -RRB- cord-320015-lbr2q4qh 265 18 into into IN cord-320015-lbr2q4qh 265 19 an an DT cord-320015-lbr2q4qh 265 20 expression expression NN cord-320015-lbr2q4qh 265 21 vector vector NN cord-320015-lbr2q4qh 265 22 and and CC cord-320015-lbr2q4qh 265 23 examined examine VBD cord-320015-lbr2q4qh 265 24 its -PRON- PRP$ cord-320015-lbr2q4qh 265 25 function function NN cord-320015-lbr2q4qh 265 26 in in IN cord-320015-lbr2q4qh 265 27 a a DT cord-320015-lbr2q4qh 265 28 yeast yeast NN cord-320015-lbr2q4qh 265 29 - - HYPH cord-320015-lbr2q4qh 265 30 based base VBN cord-320015-lbr2q4qh 265 31 assay assay NN cord-320015-lbr2q4qh 265 32 system system NN cord-320015-lbr2q4qh 265 33 . . . cord-320015-lbr2q4qh 266 1 Yeast yeast NN cord-320015-lbr2q4qh 266 2 expressing express VBG cord-320015-lbr2q4qh 266 3 human human JJ cord-320015-lbr2q4qh 266 4 PKR PKR NNP cord-320015-lbr2q4qh 266 5 failed fail VBD cord-320015-lbr2q4qh 266 6 to to TO cord-320015-lbr2q4qh 266 7 grow grow VB cord-320015-lbr2q4qh 266 8 due due IN cord-320015-lbr2q4qh 266 9 to to IN cord-320015-lbr2q4qh 266 10 the the DT cord-320015-lbr2q4qh 266 11 toxicity toxicity NN cord-320015-lbr2q4qh 266 12 of of IN cord-320015-lbr2q4qh 266 13 the the DT cord-320015-lbr2q4qh 266 14 PKR PKR NNP cord-320015-lbr2q4qh 266 15 protein protein NN cord-320015-lbr2q4qh 266 16 . . . cord-320015-lbr2q4qh 267 1 However however RB cord-320015-lbr2q4qh 267 2 , , , cord-320015-lbr2q4qh 267 3 yeast yeast NN cord-320015-lbr2q4qh 267 4 expressing express VBG cord-320015-lbr2q4qh 267 5 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 267 6 , , , cord-320015-lbr2q4qh 267 7 or or CC cord-320015-lbr2q4qh 267 8 the the DT cord-320015-lbr2q4qh 267 9 vaccinia vaccinia NN cord-320015-lbr2q4qh 267 10 virus virus NN cord-320015-lbr2q4qh 267 11 K3L k3l CD cord-320015-lbr2q4qh 267 12 protein protein NN cord-320015-lbr2q4qh 267 13 , , , cord-320015-lbr2q4qh 267 14 suppressed suppress VBD cord-320015-lbr2q4qh 267 15 the the DT cord-320015-lbr2q4qh 267 16 toxic toxic JJ cord-320015-lbr2q4qh 267 17 effects effect NNS cord-320015-lbr2q4qh 267 18 of of IN cord-320015-lbr2q4qh 267 19 human human JJ cord-320015-lbr2q4qh 267 20 PKR PKR NNP cord-320015-lbr2q4qh 267 21 indicating indicate VBG cord-320015-lbr2q4qh 267 22 that that IN cord-320015-lbr2q4qh 267 23 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 267 24 was be VBD cord-320015-lbr2q4qh 267 25 functionally functionally RB cord-320015-lbr2q4qh 267 26 equivalent equivalent JJ cord-320015-lbr2q4qh 267 27 to to IN cord-320015-lbr2q4qh 267 28 the the DT cord-320015-lbr2q4qh 267 29 vaccinia vaccinia NN cord-320015-lbr2q4qh 267 30 virus virus NN cord-320015-lbr2q4qh 267 31 protein protein NN cord-320015-lbr2q4qh 267 32 . . . cord-320015-lbr2q4qh 268 1 Subsequent subsequent JJ cord-320015-lbr2q4qh 268 2 work work NN cord-320015-lbr2q4qh 268 3 showed show VBD cord-320015-lbr2q4qh 268 4 that that IN cord-320015-lbr2q4qh 268 5 whereas whereas IN cord-320015-lbr2q4qh 268 6 K3L k3l CD cord-320015-lbr2q4qh 268 7 was be VBD cord-320015-lbr2q4qh 268 8 effective effective JJ cord-320015-lbr2q4qh 268 9 only only RB cord-320015-lbr2q4qh 268 10 against against IN cord-320015-lbr2q4qh 268 11 human human JJ cord-320015-lbr2q4qh 268 12 PKR PKR NNP cord-320015-lbr2q4qh 268 13 , , , cord-320015-lbr2q4qh 268 14 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 268 15 suppressed suppress VBD cord-320015-lbr2q4qh 268 16 the the DT cord-320015-lbr2q4qh 268 17 toxic toxic JJ cord-320015-lbr2q4qh 268 18 effects effect NNS cord-320015-lbr2q4qh 268 19 of of IN cord-320015-lbr2q4qh 268 20 both both DT cord-320015-lbr2q4qh 268 21 human human JJ cord-320015-lbr2q4qh 268 22 and and CC cord-320015-lbr2q4qh 268 23 zebrafish zebrafish NN cord-320015-lbr2q4qh 268 24 PKR PKR NNP cord-320015-lbr2q4qh 268 25 suggesting suggest VBG cord-320015-lbr2q4qh 268 26 that that IN cord-320015-lbr2q4qh 268 27 PKR PKR NNP cord-320015-lbr2q4qh 268 28 antagonists antagonist NNS cord-320015-lbr2q4qh 268 29 evolved evolve VBD cord-320015-lbr2q4qh 268 30 to to TO cord-320015-lbr2q4qh 268 31 protect protect VB cord-320015-lbr2q4qh 268 32 physiologically physiologically RB cord-320015-lbr2q4qh 268 33 - - HYPH cord-320015-lbr2q4qh 268 34 relevant relevant JJ cord-320015-lbr2q4qh 268 35 / / SYM cord-320015-lbr2q4qh 268 36 phylogeneticallyrelated phylogeneticallyrelated JJ cord-320015-lbr2q4qh 268 37 targets target NNS cord-320015-lbr2q4qh 268 38 . . . cord-320015-lbr2q4qh 269 1 In in IN cord-320015-lbr2q4qh 269 2 addition addition NN cord-320015-lbr2q4qh 269 3 , , , cord-320015-lbr2q4qh 269 4 a a DT cord-320015-lbr2q4qh 269 5 study study NN cord-320015-lbr2q4qh 269 6 using use VBG cord-320015-lbr2q4qh 269 7 vectors vector NNS cord-320015-lbr2q4qh 269 8 expressing express VBG cord-320015-lbr2q4qh 269 9 the the DT cord-320015-lbr2q4qh 269 10 various various JJ cord-320015-lbr2q4qh 269 11 domains domain NNS cord-320015-lbr2q4qh 269 12 of of IN cord-320015-lbr2q4qh 269 13 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 269 14 , , , cord-320015-lbr2q4qh 269 15 i.e. i.e. FW cord-320015-lbr2q4qh 269 16 , , , cord-320015-lbr2q4qh 269 17 N n CD cord-320015-lbr2q4qh 269 18 - - HYPH cord-320015-lbr2q4qh 269 19 terminal terminal JJ cord-320015-lbr2q4qh 269 20 , , , cord-320015-lbr2q4qh 269 21 central central JJ cord-320015-lbr2q4qh 269 22 / / SYM cord-320015-lbr2q4qh 269 23 helical helical JJ cord-320015-lbr2q4qh 269 24 , , , cord-320015-lbr2q4qh 269 25 and and CC cord-320015-lbr2q4qh 269 26 C c NN cord-320015-lbr2q4qh 269 27 - - HYPH cord-320015-lbr2q4qh 269 28 terminal terminal NN cord-320015-lbr2q4qh 269 29 , , , cord-320015-lbr2q4qh 269 30 demonstrated demonstrate VBD cord-320015-lbr2q4qh 269 31 that that IN cord-320015-lbr2q4qh 269 32 the the DT cord-320015-lbr2q4qh 269 33 N n CD cord-320015-lbr2q4qh 269 34 - - HYPH cord-320015-lbr2q4qh 269 35 terminal terminal NN cord-320015-lbr2q4qh 269 36 and and CC cord-320015-lbr2q4qh 269 37 central central JJ cord-320015-lbr2q4qh 269 38 / / SYM cord-320015-lbr2q4qh 269 39 helical helical JJ cord-320015-lbr2q4qh 269 40 domains domain NNS cord-320015-lbr2q4qh 269 41 were be VBD cord-320015-lbr2q4qh 269 42 sufficient sufficient JJ cord-320015-lbr2q4qh 269 43 for for IN cord-320015-lbr2q4qh 269 44 suppressing suppress VBG cord-320015-lbr2q4qh 269 45 PKR PKR NNP cord-320015-lbr2q4qh 269 46 toxicity toxicity NN cord-320015-lbr2q4qh 269 47 . . . cord-320015-lbr2q4qh 270 1 Collectively collectively RB cord-320015-lbr2q4qh 270 2 , , , cord-320015-lbr2q4qh 270 3 these these DT cord-320015-lbr2q4qh 270 4 results result NNS cord-320015-lbr2q4qh 270 5 provide provide VBP cord-320015-lbr2q4qh 270 6 the the DT cord-320015-lbr2q4qh 270 7 first first JJ cord-320015-lbr2q4qh 270 8 formal formal JJ cord-320015-lbr2q4qh 270 9 proof proof NN cord-320015-lbr2q4qh 270 10 that that IN cord-320015-lbr2q4qh 270 11 vIF-2α vIF-2α NNS cord-320015-lbr2q4qh 270 12 functions function NNS cord-320015-lbr2q4qh 270 13 as as IN cord-320015-lbr2q4qh 270 14 a a DT cord-320015-lbr2q4qh 270 15 virus virus NN cord-320015-lbr2q4qh 270 16 - - HYPH cord-320015-lbr2q4qh 270 17 encoded encode VBN cord-320015-lbr2q4qh 270 18 PKR PKR NNP cord-320015-lbr2q4qh 270 19 antagonist antagonist NN cord-320015-lbr2q4qh 270 20 . . . cord-320015-lbr2q4qh 271 1 Recombinant recombinant JJ cord-320015-lbr2q4qh 271 2 versions version NNS cord-320015-lbr2q4qh 271 3 of of IN cord-320015-lbr2q4qh 271 4 thymidylate thymidylate NN cord-320015-lbr2q4qh 271 5 synthase synthase NN cord-320015-lbr2q4qh 271 6 ( ( -LRB- cord-320015-lbr2q4qh 271 7 LCDV LCDV NNP cord-320015-lbr2q4qh 271 8 - - HYPH cord-320015-lbr2q4qh 271 9 China China NNP cord-320015-lbr2q4qh 271 10 ) ) -RRB- cord-320015-lbr2q4qh 271 11 , , , cord-320015-lbr2q4qh 271 12 ERV1 ERV1 NNP cord-320015-lbr2q4qh 271 13 ( ( -LRB- cord-320015-lbr2q4qh 271 14 Rana Rana NNP cord-320015-lbr2q4qh 271 15 grylio grylio NNP cord-320015-lbr2q4qh 271 16 virus virus NN cord-320015-lbr2q4qh 271 17 , , , cord-320015-lbr2q4qh 271 18 RGV RGV NNP cord-320015-lbr2q4qh 271 19 ) ) -RRB- cord-320015-lbr2q4qh 271 20 , , , cord-320015-lbr2q4qh 271 21 dUTPase dUTPase NNP cord-320015-lbr2q4qh 271 22 ( ( -LRB- cord-320015-lbr2q4qh 271 23 RGV RGV NNP cord-320015-lbr2q4qh 271 24 ) ) -RRB- cord-320015-lbr2q4qh 271 25 , , , cord-320015-lbr2q4qh 271 26 RNAse RNAse NNP cord-320015-lbr2q4qh 271 27 III III NNP cord-320015-lbr2q4qh 271 28 ( ( -LRB- cord-320015-lbr2q4qh 271 29 RBIV RBIV NNP cord-320015-lbr2q4qh 271 30 ) ) -RRB- cord-320015-lbr2q4qh 271 31 , , , cord-320015-lbr2q4qh 271 32 and and CC cord-320015-lbr2q4qh 271 33 LITAF LITAF NNP cord-320015-lbr2q4qh 271 34 ( ( -LRB- cord-320015-lbr2q4qh 271 35 SGIV SGIV NNP cord-320015-lbr2q4qh 271 36 ) ) -RRB- cord-320015-lbr2q4qh 271 37 were be VBD cord-320015-lbr2q4qh 271 38 generated generate VBN cord-320015-lbr2q4qh 271 39 and and CC cord-320015-lbr2q4qh 271 40 evaluated evaluate VBN cord-320015-lbr2q4qh 271 41 for for IN cord-320015-lbr2q4qh 271 42 their -PRON- PRP$ cord-320015-lbr2q4qh 271 43 ability ability NN cord-320015-lbr2q4qh 271 44 to to TO cord-320015-lbr2q4qh 271 45 influence influence VB cord-320015-lbr2q4qh 271 46 cellular cellular JJ cord-320015-lbr2q4qh 271 47 growth growth NN cord-320015-lbr2q4qh 271 48 , , , cord-320015-lbr2q4qh 271 49 enhance enhance VB cord-320015-lbr2q4qh 271 50 virus virus NN cord-320015-lbr2q4qh 271 51 replication replication NN cord-320015-lbr2q4qh 271 52 , , , cord-320015-lbr2q4qh 271 53 induce induce VB cord-320015-lbr2q4qh 271 54 apoptosis apoptosis NN cord-320015-lbr2q4qh 271 55 , , , cord-320015-lbr2q4qh 271 56 and and CC cord-320015-lbr2q4qh 271 57 cleave cleave VB cord-320015-lbr2q4qh 271 58 dsRNA dsRNA VBG cord-320015-lbr2q4qh 271 59 [ [ -LRB- cord-320015-lbr2q4qh 271 60 51 51 CD cord-320015-lbr2q4qh 271 61 , , , cord-320015-lbr2q4qh 271 62 75 75 CD cord-320015-lbr2q4qh 271 63 , , , cord-320015-lbr2q4qh 271 64 141 141 CD cord-320015-lbr2q4qh 271 65 , , , cord-320015-lbr2q4qh 271 66 142 142 CD cord-320015-lbr2q4qh 271 67 ] ] -RRB- cord-320015-lbr2q4qh 271 68 . . . cord-320015-lbr2q4qh 272 1 Recombinant recombinant JJ cord-320015-lbr2q4qh 272 2 thymidylate thymidylate NN cord-320015-lbr2q4qh 272 3 synthase synthase NN cord-320015-lbr2q4qh 272 4 from from IN cord-320015-lbr2q4qh 272 5 LCDV LCDV NNP cord-320015-lbr2q4qh 273 1 -China -China : cord-320015-lbr2q4qh 273 2 was be VBD cord-320015-lbr2q4qh 273 3 found find VBN cord-320015-lbr2q4qh 273 4 to to TO cord-320015-lbr2q4qh 273 5 promote promote VB cord-320015-lbr2q4qh 273 6 cell cell NN cord-320015-lbr2q4qh 273 7 cycle cycle NN cord-320015-lbr2q4qh 273 8 progression progression NN cord-320015-lbr2q4qh 273 9 and and CC cord-320015-lbr2q4qh 273 10 produced produce VBD cord-320015-lbr2q4qh 273 11 a a DT cord-320015-lbr2q4qh 273 12 transformed transform VBN cord-320015-lbr2q4qh 273 13 phenotype phenotype NN cord-320015-lbr2q4qh 273 14 [ [ -LRB- cord-320015-lbr2q4qh 273 15 143 143 CD cord-320015-lbr2q4qh 273 16 ] ] -RRB- cord-320015-lbr2q4qh 273 17 . . . cord-320015-lbr2q4qh 274 1 Antibodies antibody NNS cord-320015-lbr2q4qh 274 2 directed direct VBN cord-320015-lbr2q4qh 274 3 against against IN cord-320015-lbr2q4qh 274 4 Rana Rana NNP cord-320015-lbr2q4qh 274 5 gyrlio gyrlio NNP cord-320015-lbr2q4qh 274 6 virus virus NN cord-320015-lbr2q4qh 275 1 ( ( -LRB- cord-320015-lbr2q4qh 275 2 RGV RGV NNP cord-320015-lbr2q4qh 275 3 ) ) -RRB- cord-320015-lbr2q4qh 275 4 ERV1 ERV1 NNP cord-320015-lbr2q4qh 275 5 detected detect VBD cord-320015-lbr2q4qh 275 6 ERV1 erv1 JJ cord-320015-lbr2q4qh 275 7 expression expression NN cord-320015-lbr2q4qh 275 8 within within IN cord-320015-lbr2q4qh 275 9 the the DT cord-320015-lbr2q4qh 275 10 cytoplasm cytoplasm NN cord-320015-lbr2q4qh 275 11 and and CC cord-320015-lbr2q4qh 275 12 nucleus nucleus NN cord-320015-lbr2q4qh 275 13 , , , cord-320015-lbr2q4qh 275 14 but but CC cord-320015-lbr2q4qh 275 15 not not RB cord-320015-lbr2q4qh 275 16 within within IN cord-320015-lbr2q4qh 275 17 VAS VAS NNP cord-320015-lbr2q4qh 275 18 ; ; : cord-320015-lbr2q4qh 275 19 transcript transcript NN cord-320015-lbr2q4qh 275 20 analysis analysis NN cord-320015-lbr2q4qh 275 21 indicated indicate VBD cord-320015-lbr2q4qh 275 22 that that IN cord-320015-lbr2q4qh 275 23 ERV1 ERV1 NNP cord-320015-lbr2q4qh 275 24 was be VBD cord-320015-lbr2q4qh 275 25 a a DT cord-320015-lbr2q4qh 275 26 late late JJ cord-320015-lbr2q4qh 275 27 viral viral JJ cord-320015-lbr2q4qh 275 28 gene gene NN cord-320015-lbr2q4qh 275 29 product product NN cord-320015-lbr2q4qh 275 30 [ [ -LRB- cord-320015-lbr2q4qh 275 31 141 141 CD cord-320015-lbr2q4qh 275 32 ] ] -RRB- cord-320015-lbr2q4qh 275 33 . . . cord-320015-lbr2q4qh 276 1 RGV RGV NNP cord-320015-lbr2q4qh 276 2 dUTPase dUTPase NNP cord-320015-lbr2q4qh 276 3 was be VBD cord-320015-lbr2q4qh 276 4 determined determine VBN cord-320015-lbr2q4qh 276 5 to to TO cord-320015-lbr2q4qh 276 6 be be VB cord-320015-lbr2q4qh 276 7 a a DT cord-320015-lbr2q4qh 276 8 DE DE NNP cord-320015-lbr2q4qh 276 9 gene gene NN cord-320015-lbr2q4qh 276 10 product product NN cord-320015-lbr2q4qh 276 11 that that WDT cord-320015-lbr2q4qh 276 12 was be VBD cord-320015-lbr2q4qh 276 13 localized localize VBN cord-320015-lbr2q4qh 276 14 to to IN cord-320015-lbr2q4qh 276 15 the the DT cord-320015-lbr2q4qh 276 16 cytoplasm cytoplasm NN cord-320015-lbr2q4qh 276 17 . . . cord-320015-lbr2q4qh 277 1 Ectopic ectopic JJ cord-320015-lbr2q4qh 277 2 expression expression NN cord-320015-lbr2q4qh 277 3 did do VBD cord-320015-lbr2q4qh 277 4 not not RB cord-320015-lbr2q4qh 277 5 enhance enhance VB cord-320015-lbr2q4qh 277 6 virus virus NN cord-320015-lbr2q4qh 277 7 replication replication NN cord-320015-lbr2q4qh 277 8 , , , cord-320015-lbr2q4qh 277 9 however however RB cord-320015-lbr2q4qh 277 10 , , , cord-320015-lbr2q4qh 277 11 its -PRON- PRP$ cord-320015-lbr2q4qh 277 12 effect effect NN cord-320015-lbr2q4qh 277 13 on on IN cord-320015-lbr2q4qh 277 14 cellular cellular JJ cord-320015-lbr2q4qh 277 15 proliferation proliferation NN cord-320015-lbr2q4qh 277 16 was be VBD cord-320015-lbr2q4qh 277 17 not not RB cord-320015-lbr2q4qh 277 18 examined examine VBN cord-320015-lbr2q4qh 277 19 [ [ -LRB- cord-320015-lbr2q4qh 277 20 51 51 CD cord-320015-lbr2q4qh 277 21 ] ] -RRB- cord-320015-lbr2q4qh 277 22 . . . cord-320015-lbr2q4qh 278 1 Recombinant Recombinant NNP cord-320015-lbr2q4qh 278 2 RNAse RNAse NNP cord-320015-lbr2q4qh 278 3 III III NNP cord-320015-lbr2q4qh 278 4 from from IN cord-320015-lbr2q4qh 278 5 RBIV RBIV NNP cord-320015-lbr2q4qh 278 6 cleaved cleave VBD cord-320015-lbr2q4qh 278 7 dsRNA dsrna NN cord-320015-lbr2q4qh 278 8 , , , cord-320015-lbr2q4qh 278 9 but but CC cord-320015-lbr2q4qh 278 10 the the DT cord-320015-lbr2q4qh 278 11 salt salt NN cord-320015-lbr2q4qh 278 12 optimum optimum NN cord-320015-lbr2q4qh 278 13 for for IN cord-320015-lbr2q4qh 278 14 cleavage cleavage NN cord-320015-lbr2q4qh 278 15 was be VBD cord-320015-lbr2q4qh 278 16 inconsistent inconsistent JJ cord-320015-lbr2q4qh 278 17 with with IN cord-320015-lbr2q4qh 278 18 a a DT cord-320015-lbr2q4qh 278 19 physiological physiological JJ cord-320015-lbr2q4qh 278 20 effect effect NN cord-320015-lbr2q4qh 278 21 [ [ -LRB- cord-320015-lbr2q4qh 278 22 75 75 CD cord-320015-lbr2q4qh 278 23 ] ] -RRB- cord-320015-lbr2q4qh 278 24 . . . cord-320015-lbr2q4qh 279 1 The the DT cord-320015-lbr2q4qh 279 2 authors author NNS cord-320015-lbr2q4qh 279 3 speculated speculate VBD cord-320015-lbr2q4qh 279 4 that that IN cord-320015-lbr2q4qh 279 5 RNAse RNAse NNP cord-320015-lbr2q4qh 279 6 III III NNP cord-320015-lbr2q4qh 279 7 might may MD cord-320015-lbr2q4qh 279 8 play play VB cord-320015-lbr2q4qh 279 9 a a DT cord-320015-lbr2q4qh 279 10 role role NN cord-320015-lbr2q4qh 279 11 in in IN cord-320015-lbr2q4qh 279 12 the the DT cord-320015-lbr2q4qh 279 13 suppression suppression NN cord-320015-lbr2q4qh 279 14 of of IN cord-320015-lbr2q4qh 279 15 RNA RNA NNP cord-320015-lbr2q4qh 279 16 interference interference NN cord-320015-lbr2q4qh 279 17 , , , cord-320015-lbr2q4qh 279 18 as as IN cord-320015-lbr2q4qh 279 19 has have VBZ cord-320015-lbr2q4qh 279 20 been be VBN cord-320015-lbr2q4qh 279 21 suggested suggest VBN cord-320015-lbr2q4qh 279 22 for for IN cord-320015-lbr2q4qh 279 23 other other JJ cord-320015-lbr2q4qh 279 24 viral viral JJ cord-320015-lbr2q4qh 279 25 dsRNA dsrna RB cord-320015-lbr2q4qh 279 26 - - HYPH cord-320015-lbr2q4qh 279 27 binding bind VBG cord-320015-lbr2q4qh 279 28 proteins protein NNS cord-320015-lbr2q4qh 279 29 , , , cord-320015-lbr2q4qh 279 30 or or CC cord-320015-lbr2q4qh 279 31 could could MD cord-320015-lbr2q4qh 279 32 be be VB cord-320015-lbr2q4qh 279 33 involved involve VBN cord-320015-lbr2q4qh 279 34 in in IN cord-320015-lbr2q4qh 279 35 the the DT cord-320015-lbr2q4qh 279 36 processing processing NN cord-320015-lbr2q4qh 279 37 of of IN cord-320015-lbr2q4qh 279 38 viral viral JJ cord-320015-lbr2q4qh 279 39 RNA RNA NNP cord-320015-lbr2q4qh 279 40 . . . cord-320015-lbr2q4qh 280 1 Lastly lastly RB cord-320015-lbr2q4qh 280 2 , , , cord-320015-lbr2q4qh 280 3 Huang Huang NNP cord-320015-lbr2q4qh 280 4 et et NNP cord-320015-lbr2q4qh 280 5 al al NNP cord-320015-lbr2q4qh 280 6 . . . cord-320015-lbr2q4qh 281 1 [ [ -LRB- cord-320015-lbr2q4qh 281 2 142 142 CD cord-320015-lbr2q4qh 281 3 ] ] -RRB- cord-320015-lbr2q4qh 281 4 demonstrated demonstrate VBD cord-320015-lbr2q4qh 281 5 that that IN cord-320015-lbr2q4qh 281 6 the the DT cord-320015-lbr2q4qh 281 7 SGIV SGIV NNP cord-320015-lbr2q4qh 281 8 homolog homolog NN cord-320015-lbr2q4qh 281 9 of of IN cord-320015-lbr2q4qh 281 10 LITAF LITAF NNP cord-320015-lbr2q4qh 281 11 ( ( -LRB- cord-320015-lbr2q4qh 281 12 lipopolysaccharide lipopolysaccharide NN cord-320015-lbr2q4qh 281 13 - - HYPH cord-320015-lbr2q4qh 281 14 induced induce VBN cord-320015-lbr2q4qh 281 15 TNF TNF NNP cord-320015-lbr2q4qh 281 16 - - HYPH cord-320015-lbr2q4qh 281 17 α α NN cord-320015-lbr2q4qh 281 18 factor factor NN cord-320015-lbr2q4qh 281 19 ) ) -RRB- cord-320015-lbr2q4qh 281 20 was be VBD cord-320015-lbr2q4qh 281 21 a a DT cord-320015-lbr2q4qh 281 22 DE DE NNP cord-320015-lbr2q4qh 281 23 viral viral JJ cord-320015-lbr2q4qh 281 24 gene gene NN cord-320015-lbr2q4qh 281 25 product product NN cord-320015-lbr2q4qh 281 26 . . . cord-320015-lbr2q4qh 282 1 LITAF LITAF NNP cord-320015-lbr2q4qh 282 2 was be VBD cord-320015-lbr2q4qh 282 3 located locate VBN cord-320015-lbr2q4qh 282 4 within within IN cord-320015-lbr2q4qh 282 5 the the DT cord-320015-lbr2q4qh 282 6 cytoplasm cytoplasm NN cord-320015-lbr2q4qh 282 7 and and CC cord-320015-lbr2q4qh 282 8 was be VBD cord-320015-lbr2q4qh 282 9 associated associate VBN cord-320015-lbr2q4qh 282 10 with with IN cord-320015-lbr2q4qh 282 11 mitochondria mitochondrion NNS cord-320015-lbr2q4qh 282 12 . . . cord-320015-lbr2q4qh 283 1 When when WRB cord-320015-lbr2q4qh 283 2 over over RB cord-320015-lbr2q4qh 283 3 - - HYPH cord-320015-lbr2q4qh 283 4 expressed express VBN cord-320015-lbr2q4qh 283 5 ectopically ectopically RB cord-320015-lbr2q4qh 283 6 , , , cord-320015-lbr2q4qh 283 7 it -PRON- PRP cord-320015-lbr2q4qh 283 8 led lead VBD cord-320015-lbr2q4qh 283 9 to to IN cord-320015-lbr2q4qh 283 10 the the DT cord-320015-lbr2q4qh 283 11 activation activation NN cord-320015-lbr2q4qh 283 12 of of IN cord-320015-lbr2q4qh 283 13 caspase caspase NN cord-320015-lbr2q4qh 283 14 3 3 CD cord-320015-lbr2q4qh 283 15 and and CC cord-320015-lbr2q4qh 283 16 the the DT cord-320015-lbr2q4qh 283 17 induction induction NN cord-320015-lbr2q4qh 283 18 of of IN cord-320015-lbr2q4qh 283 19 apoptosis apoptosis NN cord-320015-lbr2q4qh 283 20 . . . cord-320015-lbr2q4qh 284 1 Collectively collectively RB cord-320015-lbr2q4qh 284 2 , , , cord-320015-lbr2q4qh 284 3 ts ts NNP cord-320015-lbr2q4qh 284 4 mutants mutant NNS cord-320015-lbr2q4qh 284 5 , , , cord-320015-lbr2q4qh 284 6 asMO asmo NN cord-320015-lbr2q4qh 284 7 - - HYPH cord-320015-lbr2q4qh 284 8 and and CC cord-320015-lbr2q4qh 284 9 siRNA siRNA NNP cord-320015-lbr2q4qh 284 10 - - HYPH cord-320015-lbr2q4qh 284 11 mediated mediate VBN cord-320015-lbr2q4qh 284 12 knock knock NN cord-320015-lbr2q4qh 284 13 down down RP cord-320015-lbr2q4qh 284 14 , , , cord-320015-lbr2q4qh 284 15 gene gene NN cord-320015-lbr2q4qh 284 16 knock knock VB cord-320015-lbr2q4qh 284 17 out out RP cord-320015-lbr2q4qh 284 18 , , , cord-320015-lbr2q4qh 284 19 and and CC cord-320015-lbr2q4qh 284 20 recombinant recombinant JJ cord-320015-lbr2q4qh 284 21 protein protein NN cord-320015-lbr2q4qh 284 22 studies study NNS cord-320015-lbr2q4qh 284 23 are be VBP cord-320015-lbr2q4qh 284 24 slowly slowly RB cord-320015-lbr2q4qh 284 25 elucidating elucidate VBG cord-320015-lbr2q4qh 284 26 the the DT cord-320015-lbr2q4qh 284 27 function function NN cord-320015-lbr2q4qh 284 28 of of IN cord-320015-lbr2q4qh 284 29 iridovirid iridovirid NNP cord-320015-lbr2q4qh 284 30 genes gene NNS cord-320015-lbr2q4qh 284 31 and and CC cord-320015-lbr2q4qh 284 32 providing provide VBG cord-320015-lbr2q4qh 284 33 a a DT cord-320015-lbr2q4qh 284 34 clearer clear JJR cord-320015-lbr2q4qh 284 35 and and CC cord-320015-lbr2q4qh 284 36 more more RBR cord-320015-lbr2q4qh 284 37 complete complete JJ cord-320015-lbr2q4qh 284 38 picture picture NN cord-320015-lbr2q4qh 284 39 of of IN cord-320015-lbr2q4qh 284 40 how how WRB cord-320015-lbr2q4qh 284 41 those those DT cord-320015-lbr2q4qh 284 42 genes gene NNS cord-320015-lbr2q4qh 284 43 enhance enhance VBP cord-320015-lbr2q4qh 284 44 viral viral JJ cord-320015-lbr2q4qh 284 45 replication replication NN cord-320015-lbr2q4qh 284 46 and and CC cord-320015-lbr2q4qh 284 47 modulate modulate VB cord-320015-lbr2q4qh 284 48 cellular cellular JJ cord-320015-lbr2q4qh 284 49 functions function NNS cord-320015-lbr2q4qh 284 50 . . . cord-320015-lbr2q4qh 285 1 Future future JJ cord-320015-lbr2q4qh 285 2 studies study NNS cord-320015-lbr2q4qh 285 3 of of IN cord-320015-lbr2q4qh 285 4 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 285 5 and and CC cord-320015-lbr2q4qh 285 6 other other JJ cord-320015-lbr2q4qh 285 7 iridoviruses iridoviruse NNS cord-320015-lbr2q4qh 285 8 infecting infect VBG cord-320015-lbr2q4qh 285 9 ectothermic ectothermic JJ cord-320015-lbr2q4qh 285 10 animals animal NNS cord-320015-lbr2q4qh 285 11 will will MD cord-320015-lbr2q4qh 285 12 focus focus VB cord-320015-lbr2q4qh 285 13 on on IN cord-320015-lbr2q4qh 285 14 the the DT cord-320015-lbr2q4qh 285 15 following follow VBG cord-320015-lbr2q4qh 285 16 areas area NNS cord-320015-lbr2q4qh 285 17 : : : cord-320015-lbr2q4qh 285 18 ( ( -LRB- cord-320015-lbr2q4qh 285 19 1 1 LS cord-320015-lbr2q4qh 285 20 ) ) -RRB- cord-320015-lbr2q4qh 285 21 identifying identify VBG cord-320015-lbr2q4qh 285 22 and and CC cord-320015-lbr2q4qh 285 23 elucidating elucidate VBG cord-320015-lbr2q4qh 285 24 the the DT cord-320015-lbr2q4qh 285 25 function function NN cord-320015-lbr2q4qh 285 26 of of IN cord-320015-lbr2q4qh 285 27 replicative replicative JJ cord-320015-lbr2q4qh 285 28 genes gene NNS cord-320015-lbr2q4qh 285 29 and and CC cord-320015-lbr2q4qh 285 30 those those DT cord-320015-lbr2q4qh 285 31 contributing contribute VBG cord-320015-lbr2q4qh 285 32 to to IN cord-320015-lbr2q4qh 285 33 virulence virulence NN cord-320015-lbr2q4qh 285 34 , , , cord-320015-lbr2q4qh 285 35 host host NN cord-320015-lbr2q4qh 285 36 range range NN cord-320015-lbr2q4qh 285 37 , , , cord-320015-lbr2q4qh 285 38 and and CC cord-320015-lbr2q4qh 285 39 immune immune JJ cord-320015-lbr2q4qh 285 40 evasion evasion NN cord-320015-lbr2q4qh 285 41 ; ; : cord-320015-lbr2q4qh 285 42 ( ( -LRB- cord-320015-lbr2q4qh 285 43 2 2 LS cord-320015-lbr2q4qh 285 44 ) ) -RRB- cord-320015-lbr2q4qh 285 45 understanding understand VBG cord-320015-lbr2q4qh 285 46 the the DT cord-320015-lbr2q4qh 285 47 correlates correlate NNS cord-320015-lbr2q4qh 285 48 of of IN cord-320015-lbr2q4qh 285 49 antiviral antiviral JJ cord-320015-lbr2q4qh 285 50 immunity immunity NN cord-320015-lbr2q4qh 285 51 in in IN cord-320015-lbr2q4qh 285 52 an an DT cord-320015-lbr2q4qh 285 53 effort effort NN cord-320015-lbr2q4qh 285 54 to to TO cord-320015-lbr2q4qh 285 55 understand understand VB cord-320015-lbr2q4qh 285 56 the the DT cord-320015-lbr2q4qh 285 57 cellular cellular JJ cord-320015-lbr2q4qh 285 58 and and CC cord-320015-lbr2q4qh 285 59 molecular molecular JJ cord-320015-lbr2q4qh 285 60 basis basis NN cord-320015-lbr2q4qh 285 61 of of IN cord-320015-lbr2q4qh 285 62 anti anti JJ cord-320015-lbr2q4qh 285 63 - - JJ cord-320015-lbr2q4qh 285 64 viral viral JJ cord-320015-lbr2q4qh 285 65 immunity immunity NN cord-320015-lbr2q4qh 285 66 and and CC cord-320015-lbr2q4qh 285 67 to to TO cord-320015-lbr2q4qh 285 68 protect protect VB cord-320015-lbr2q4qh 285 69 endangered endanger VBN cord-320015-lbr2q4qh 285 70 and and CC cord-320015-lbr2q4qh 285 71 commercially commercially RB cord-320015-lbr2q4qh 285 72 important important JJ cord-320015-lbr2q4qh 285 73 species specie NNS cord-320015-lbr2q4qh 285 74 from from IN cord-320015-lbr2q4qh 285 75 infection infection NN cord-320015-lbr2q4qh 285 76 ; ; , cord-320015-lbr2q4qh 285 77 ( ( -LRB- cord-320015-lbr2q4qh 285 78 3 3 LS cord-320015-lbr2q4qh 285 79 ) ) -RRB- cord-320015-lbr2q4qh 285 80 determining determine VBG cord-320015-lbr2q4qh 285 81 the the DT cord-320015-lbr2q4qh 285 82 impact impact NN cord-320015-lbr2q4qh 285 83 of of IN cord-320015-lbr2q4qh 285 84 iridovirus iridovirus JJ cord-320015-lbr2q4qh 285 85 infections infection NNS cord-320015-lbr2q4qh 285 86 in in IN cord-320015-lbr2q4qh 285 87 nature nature NN cord-320015-lbr2q4qh 285 88 , , , cord-320015-lbr2q4qh 285 89 defining define VBG cord-320015-lbr2q4qh 285 90 viral viral JJ cord-320015-lbr2q4qh 285 91 host host NN cord-320015-lbr2q4qh 285 92 range range NN cord-320015-lbr2q4qh 285 93 , , , cord-320015-lbr2q4qh 285 94 identifying identify VBG cord-320015-lbr2q4qh 285 95 susceptible susceptible JJ cord-320015-lbr2q4qh 285 96 hosts host NNS cord-320015-lbr2q4qh 285 97 and and CC cord-320015-lbr2q4qh 285 98 reservoir reservoir NN cord-320015-lbr2q4qh 285 99 species specie NNS cord-320015-lbr2q4qh 285 100 ; ; , cord-320015-lbr2q4qh 285 101 ( ( -LRB- cord-320015-lbr2q4qh 285 102 4 4 LS cord-320015-lbr2q4qh 285 103 ) ) -RRB- cord-320015-lbr2q4qh 285 104 understanding understand VBG cord-320015-lbr2q4qh 285 105 how how WRB cord-320015-lbr2q4qh 285 106 intrinsic intrinsic JJ cord-320015-lbr2q4qh 285 107 ( ( -LRB- cord-320015-lbr2q4qh 285 108 e.g. e.g. RB cord-320015-lbr2q4qh 285 109 , , , cord-320015-lbr2q4qh 285 110 host host NN cord-320015-lbr2q4qh 285 111 immune immune JJ cord-320015-lbr2q4qh 285 112 suppression suppression NN cord-320015-lbr2q4qh 285 113 , , , cord-320015-lbr2q4qh 285 114 host host NN cord-320015-lbr2q4qh 285 115 MHC MHC NNP cord-320015-lbr2q4qh 285 116 repertoire repertoire NN cord-320015-lbr2q4qh 285 117 , , , cord-320015-lbr2q4qh 285 118 etc etc FW cord-320015-lbr2q4qh 285 119 . . . cord-320015-lbr2q4qh 285 120 ) ) -RRB- cord-320015-lbr2q4qh 286 1 and and CC cord-320015-lbr2q4qh 286 2 extrinsic extrinsic JJ cord-320015-lbr2q4qh 286 3 ( ( -LRB- cord-320015-lbr2q4qh 286 4 e.g. e.g. RB cord-320015-lbr2q4qh 286 5 , , , cord-320015-lbr2q4qh 286 6 habitat habitat NN cord-320015-lbr2q4qh 286 7 disruption disruption NN cord-320015-lbr2q4qh 286 8 , , , cord-320015-lbr2q4qh 286 9 environmental environmental JJ cord-320015-lbr2q4qh 286 10 stress stress NN cord-320015-lbr2q4qh 286 11 , , , cord-320015-lbr2q4qh 286 12 introduction introduction NN cord-320015-lbr2q4qh 286 13 of of IN cord-320015-lbr2q4qh 286 14 invasive invasive JJ cord-320015-lbr2q4qh 286 15 species specie NNS cord-320015-lbr2q4qh 286 16 , , , cord-320015-lbr2q4qh 286 17 etc etc FW cord-320015-lbr2q4qh 286 18 . . . cord-320015-lbr2q4qh 286 19 ) ) -RRB- cord-320015-lbr2q4qh 287 1 factors factor NNS cord-320015-lbr2q4qh 287 2 influence influence VBP cord-320015-lbr2q4qh 287 3 the the DT cord-320015-lbr2q4qh 287 4 clinical clinical JJ cord-320015-lbr2q4qh 287 5 outcomes outcome NNS cord-320015-lbr2q4qh 287 6 of of IN cord-320015-lbr2q4qh 287 7 iridovirus iridovirus JJ cord-320015-lbr2q4qh 287 8 infection infection NN cord-320015-lbr2q4qh 287 9 . . . cord-320015-lbr2q4qh 288 1 For for IN cord-320015-lbr2q4qh 288 2 example example NN cord-320015-lbr2q4qh 288 3 , , , cord-320015-lbr2q4qh 288 4 determining determine VBG cord-320015-lbr2q4qh 288 5 whether whether IN cord-320015-lbr2q4qh 288 6 iridovirids iridovirid NNS cord-320015-lbr2q4qh 288 7 encode encode VB cord-320015-lbr2q4qh 288 8 viral viral JJ cord-320015-lbr2q4qh 288 9 immune immune JJ cord-320015-lbr2q4qh 288 10 evasion evasion NN cord-320015-lbr2q4qh 288 11 proteins protein NNS cord-320015-lbr2q4qh 288 12 and and CC cord-320015-lbr2q4qh 288 13 how how WRB cord-320015-lbr2q4qh 288 14 these these DT cord-320015-lbr2q4qh 288 15 proteins protein NNS cord-320015-lbr2q4qh 288 16 circumvent circumvent VBP cord-320015-lbr2q4qh 288 17 host host NN cord-320015-lbr2q4qh 288 18 immunity immunity NN cord-320015-lbr2q4qh 288 19 will will MD cord-320015-lbr2q4qh 288 20 highlight highlight VB cord-320015-lbr2q4qh 288 21 events event NNS cord-320015-lbr2q4qh 288 22 at at IN cord-320015-lbr2q4qh 288 23 the the DT cord-320015-lbr2q4qh 288 24 interface interface NN cord-320015-lbr2q4qh 288 25 of of IN cord-320015-lbr2q4qh 288 26 virology virology NN cord-320015-lbr2q4qh 288 27 and and CC cord-320015-lbr2q4qh 288 28 immunology immunology NN cord-320015-lbr2q4qh 288 29 , , , cord-320015-lbr2q4qh 288 30 and and CC cord-320015-lbr2q4qh 288 31 may may MD cord-320015-lbr2q4qh 288 32 identify identify VB cord-320015-lbr2q4qh 288 33 possible possible JJ cord-320015-lbr2q4qh 288 34 targets target NNS cord-320015-lbr2q4qh 288 35 for for IN cord-320015-lbr2q4qh 288 36 viral viral JJ cord-320015-lbr2q4qh 288 37 attenuation attenuation NN cord-320015-lbr2q4qh 288 38 . . . cord-320015-lbr2q4qh 289 1 Successful successful JJ cord-320015-lbr2q4qh 289 2 completion completion NN cord-320015-lbr2q4qh 289 3 of of IN cord-320015-lbr2q4qh 289 4 these these DT cord-320015-lbr2q4qh 289 5 studies study NNS cord-320015-lbr2q4qh 289 6 will will MD cord-320015-lbr2q4qh 289 7 involve involve VB cord-320015-lbr2q4qh 289 8 the the DT cord-320015-lbr2q4qh 289 9 interactive interactive JJ cord-320015-lbr2q4qh 289 10 efforts effort NNS cord-320015-lbr2q4qh 289 11 of of IN cord-320015-lbr2q4qh 289 12 virologists virologist NNS cord-320015-lbr2q4qh 289 13 , , , cord-320015-lbr2q4qh 289 14 immunologists immunologist NNS cord-320015-lbr2q4qh 289 15 , , , cord-320015-lbr2q4qh 289 16 population population NN cord-320015-lbr2q4qh 289 17 biologists biologist NNS cord-320015-lbr2q4qh 289 18 , , , cord-320015-lbr2q4qh 289 19 ecologists ecologist NNS cord-320015-lbr2q4qh 289 20 , , , cord-320015-lbr2q4qh 289 21 and and CC cord-320015-lbr2q4qh 289 22 veterinary veterinary JJ cord-320015-lbr2q4qh 289 23 pathologists pathologist NNS cord-320015-lbr2q4qh 289 24 . . . cord-320015-lbr2q4qh 290 1 The the DT cord-320015-lbr2q4qh 290 2 Global Global NNP cord-320015-lbr2q4qh 290 3 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 290 4 Consortium Consortium NNP cord-320015-lbr2q4qh 290 5 [ [ -LRB- cord-320015-lbr2q4qh 290 6 144 144 CD cord-320015-lbr2q4qh 290 7 ] ] -RRB- cord-320015-lbr2q4qh 290 8 is be VBZ cord-320015-lbr2q4qh 290 9 but but CC cord-320015-lbr2q4qh 290 10 one one CD cord-320015-lbr2q4qh 290 11 interactive interactive JJ cord-320015-lbr2q4qh 290 12 group group NN cord-320015-lbr2q4qh 290 13 of of IN cord-320015-lbr2q4qh 290 14 scientists scientist NNS cord-320015-lbr2q4qh 290 15 interested interested JJ cord-320015-lbr2q4qh 290 16 in in IN cord-320015-lbr2q4qh 290 17 the the DT cord-320015-lbr2q4qh 290 18 role role NN cord-320015-lbr2q4qh 290 19 of of IN cord-320015-lbr2q4qh 290 20 infectious infectious JJ cord-320015-lbr2q4qh 290 21 agents agent NNS cord-320015-lbr2q4qh 290 22 in in IN cord-320015-lbr2q4qh 290 23 population population NN cord-320015-lbr2q4qh 290 24 declines decline VBZ cord-320015-lbr2q4qh 290 25 . . . cord-320015-lbr2q4qh 291 1 Moreover moreover RB cord-320015-lbr2q4qh 291 2 , , , cord-320015-lbr2q4qh 291 3 it -PRON- PRP cord-320015-lbr2q4qh 291 4 is be VBZ cord-320015-lbr2q4qh 291 5 anticipated anticipate VBN cord-320015-lbr2q4qh 291 6 that that IN cord-320015-lbr2q4qh 291 7 general general JJ cord-320015-lbr2q4qh 291 8 principles principle NNS cord-320015-lbr2q4qh 291 9 learned learn VBD cord-320015-lbr2q4qh 291 10 through through IN cord-320015-lbr2q4qh 291 11 study study NN cord-320015-lbr2q4qh 291 12 of of IN cord-320015-lbr2q4qh 291 13 iridovirus iridovirus NNP cord-320015-lbr2q4qh 291 14 infections infection NNS cord-320015-lbr2q4qh 291 15 will will MD cord-320015-lbr2q4qh 291 16 be be VB cord-320015-lbr2q4qh 291 17 applicable applicable JJ cord-320015-lbr2q4qh 291 18 to to IN cord-320015-lbr2q4qh 291 19 the the DT cord-320015-lbr2q4qh 291 20 study study NN cord-320015-lbr2q4qh 291 21 of of IN cord-320015-lbr2q4qh 291 22 other other JJ cord-320015-lbr2q4qh 291 23 pathogens pathogen NNS cord-320015-lbr2q4qh 291 24 of of IN cord-320015-lbr2q4qh 291 25 cold cold JJ cord-320015-lbr2q4qh 291 26 - - HYPH cord-320015-lbr2q4qh 291 27 blooded blooded JJ cord-320015-lbr2q4qh 291 28 vertebrates vertebrate NNS cord-320015-lbr2q4qh 291 29 . . . cord-320015-lbr2q4qh 292 1 Ranaviruses Ranaviruses NNP cord-320015-lbr2q4qh 292 2 ( ( -LRB- cord-320015-lbr2q4qh 292 3 family family NN cord-320015-lbr2q4qh 292 4 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 292 5 ) ) -RRB- cord-320015-lbr2q4qh 292 6 : : : cord-320015-lbr2q4qh 292 7 emerging emerge VBG cord-320015-lbr2q4qh 292 8 cold cold JJ cord-320015-lbr2q4qh 292 9 - - HYPH cord-320015-lbr2q4qh 292 10 blooded blooded JJ cord-320015-lbr2q4qh 292 11 killers killer NNS cord-320015-lbr2q4qh 292 12 Family Family NNP cord-320015-lbr2q4qh 292 13 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 292 14 : : : cord-320015-lbr2q4qh 292 15 poor poor JJ cord-320015-lbr2q4qh 292 16 viral viral JJ cord-320015-lbr2q4qh 292 17 relations relation NNS cord-320015-lbr2q4qh 292 18 no no DT cord-320015-lbr2q4qh 292 19 longer long RBR cord-320015-lbr2q4qh 292 20 Evolutionary evolutionary JJ cord-320015-lbr2q4qh 292 21 genomics genomic NNS cord-320015-lbr2q4qh 292 22 of of IN cord-320015-lbr2q4qh 292 23 nucleo nucleo JJ cord-320015-lbr2q4qh 292 24 - - JJ cord-320015-lbr2q4qh 292 25 cytoplasmic cytoplasmic JJ cord-320015-lbr2q4qh 292 26 large large JJ cord-320015-lbr2q4qh 292 27 DNA dna NN cord-320015-lbr2q4qh 292 28 viruses virus NNS cord-320015-lbr2q4qh 292 29 Common common JJ cord-320015-lbr2q4qh 292 30 origin origin NN cord-320015-lbr2q4qh 292 31 of of IN cord-320015-lbr2q4qh 292 32 four four CD cord-320015-lbr2q4qh 292 33 diverse diverse JJ cord-320015-lbr2q4qh 292 34 families family NNS cord-320015-lbr2q4qh 292 35 of of IN cord-320015-lbr2q4qh 292 36 large large JJ cord-320015-lbr2q4qh 292 37 eukaryotic eukaryotic JJ cord-320015-lbr2q4qh 292 38 DNA dna NN cord-320015-lbr2q4qh 292 39 viruses virus NNS cord-320015-lbr2q4qh 292 40 Evolution evolution NN cord-320015-lbr2q4qh 292 41 of of IN cord-320015-lbr2q4qh 292 42 DNA dna NN cord-320015-lbr2q4qh 292 43 ligases ligase NNS cord-320015-lbr2q4qh 292 44 of of IN cord-320015-lbr2q4qh 292 45 nucleo nucleo JJ cord-320015-lbr2q4qh 292 46 - - JJ cord-320015-lbr2q4qh 292 47 cytoplasmic cytoplasmic JJ cord-320015-lbr2q4qh 292 48 large large JJ cord-320015-lbr2q4qh 292 49 DNA dna NN cord-320015-lbr2q4qh 292 50 viruses virus NNS cord-320015-lbr2q4qh 292 51 of of IN cord-320015-lbr2q4qh 292 52 eukaryotes eukaryote NNS cord-320015-lbr2q4qh 292 53 : : : cord-320015-lbr2q4qh 292 54 a a DT cord-320015-lbr2q4qh 292 55 case case NN cord-320015-lbr2q4qh 292 56 of of IN cord-320015-lbr2q4qh 292 57 hidden hide VBN cord-320015-lbr2q4qh 292 58 complexity complexity NN cord-320015-lbr2q4qh 292 59 Eukaryotic eukaryotic JJ cord-320015-lbr2q4qh 292 60 large large JJ cord-320015-lbr2q4qh 292 61 nucleo nucleo JJ cord-320015-lbr2q4qh 292 62 - - JJ cord-320015-lbr2q4qh 292 63 cytoplasmic cytoplasmic JJ cord-320015-lbr2q4qh 292 64 DNA dna NN cord-320015-lbr2q4qh 292 65 viruses virus NNS cord-320015-lbr2q4qh 292 66 : : : cord-320015-lbr2q4qh 292 67 Clusters cluster NNS cord-320015-lbr2q4qh 292 68 of of IN cord-320015-lbr2q4qh 292 69 orthologous orthologous JJ cord-320015-lbr2q4qh 292 70 genes gene NNS cord-320015-lbr2q4qh 292 71 and and CC cord-320015-lbr2q4qh 292 72 reconstruction reconstruction NN cord-320015-lbr2q4qh 292 73 of of IN cord-320015-lbr2q4qh 292 74 viral viral JJ cord-320015-lbr2q4qh 292 75 genome genome NN cord-320015-lbr2q4qh 292 76 evolution evolution NN cord-320015-lbr2q4qh 292 77 Functional functional JJ cord-320015-lbr2q4qh 292 78 genomics genomic NNS cord-320015-lbr2q4qh 292 79 analysis analysis NN cord-320015-lbr2q4qh 292 80 of of IN cord-320015-lbr2q4qh 292 81 Singapore Singapore NNP cord-320015-lbr2q4qh 292 82 grouper grouper NN cord-320015-lbr2q4qh 292 83 iridovirus iridovirus NN cord-320015-lbr2q4qh 292 84 : : : cord-320015-lbr2q4qh 292 85 Complete complete JJ cord-320015-lbr2q4qh 292 86 sequence sequence NN cord-320015-lbr2q4qh 292 87 determination determination NN cord-320015-lbr2q4qh 292 88 and and CC cord-320015-lbr2q4qh 292 89 proteomic proteomic JJ cord-320015-lbr2q4qh 292 90 analysis analysis NN cord-320015-lbr2q4qh 292 91 Occurrence occurrence NN cord-320015-lbr2q4qh 292 92 of of IN cord-320015-lbr2q4qh 292 93 an an DT cord-320015-lbr2q4qh 292 94 invertebrate invertebrate JJ cord-320015-lbr2q4qh 292 95 iridescent iridescent NN cord-320015-lbr2q4qh 292 96 - - HYPH cord-320015-lbr2q4qh 292 97 like like JJ cord-320015-lbr2q4qh 292 98 virus virus NN cord-320015-lbr2q4qh 292 99 ( ( -LRB- cord-320015-lbr2q4qh 292 100 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 292 101 ) ) -RRB- cord-320015-lbr2q4qh 292 102 in in IN cord-320015-lbr2q4qh 292 103 reptiles reptile NNS cord-320015-lbr2q4qh 292 104 Experimental experimental JJ cord-320015-lbr2q4qh 292 105 infection infection NN cord-320015-lbr2q4qh 292 106 of of IN cord-320015-lbr2q4qh 292 107 crickets cricket NNS cord-320015-lbr2q4qh 292 108 ( ( -LRB- cord-320015-lbr2q4qh 292 109 Gryllus Gryllus NNP cord-320015-lbr2q4qh 292 110 bimaculatus bimaculatus NNP cord-320015-lbr2q4qh 292 111 ) ) -RRB- cord-320015-lbr2q4qh 292 112 with with IN cord-320015-lbr2q4qh 292 113 an an DT cord-320015-lbr2q4qh 292 114 invertebrate invertebrate JJ cord-320015-lbr2q4qh 292 115 iridovirus iridovirus NN cord-320015-lbr2q4qh 292 116 isolated isolate VBN cord-320015-lbr2q4qh 292 117 from from IN cord-320015-lbr2q4qh 292 118 the the DT cord-320015-lbr2q4qh 292 119 high high JJ cord-320015-lbr2q4qh 292 120 casqued casque VBN cord-320015-lbr2q4qh 292 121 chameleon chameleon NN cord-320015-lbr2q4qh 292 122 ( ( -LRB- cord-320015-lbr2q4qh 292 123 Chamaeleo Chamaeleo NNP cord-320015-lbr2q4qh 292 124 hoenelii hoenelii NN cord-320015-lbr2q4qh 292 125 ) ) -RRB- cord-320015-lbr2q4qh 293 1 Comparative comparative JJ cord-320015-lbr2q4qh 293 2 genomic genomic JJ cord-320015-lbr2q4qh 293 3 analysis analysis NN cord-320015-lbr2q4qh 293 4 of of IN cord-320015-lbr2q4qh 293 5 the the DT cord-320015-lbr2q4qh 293 6 family family NN cord-320015-lbr2q4qh 293 7 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 293 8 : : : cord-320015-lbr2q4qh 293 9 Re re NN cord-320015-lbr2q4qh 293 10 - - NN cord-320015-lbr2q4qh 293 11 annotating annotate VBG cord-320015-lbr2q4qh 293 12 and and CC cord-320015-lbr2q4qh 293 13 defining define VBG cord-320015-lbr2q4qh 293 14 the the DT cord-320015-lbr2q4qh 293 15 core core NN cord-320015-lbr2q4qh 293 16 set set NN cord-320015-lbr2q4qh 293 17 of of IN cord-320015-lbr2q4qh 293 18 iridovirus iridovirus JJ cord-320015-lbr2q4qh 293 19 genes gene NNS cord-320015-lbr2q4qh 293 20 Evidence evidence NN cord-320015-lbr2q4qh 293 21 for for IN cord-320015-lbr2q4qh 293 22 multiple multiple JJ cord-320015-lbr2q4qh 293 23 recent recent JJ cord-320015-lbr2q4qh 293 24 host host NN cord-320015-lbr2q4qh 293 25 species species NN cord-320015-lbr2q4qh 293 26 shifts shift NNS cord-320015-lbr2q4qh 293 27 among among IN cord-320015-lbr2q4qh 293 28 the the DT cord-320015-lbr2q4qh 293 29 Ranaviruses Ranaviruses NNPS cord-320015-lbr2q4qh 293 30 ( ( -LRB- cord-320015-lbr2q4qh 293 31 family family NN cord-320015-lbr2q4qh 293 32 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 293 33 ) ) -RRB- cord-320015-lbr2q4qh 293 34 Genomic genomic JJ cord-320015-lbr2q4qh 293 35 and and CC cord-320015-lbr2q4qh 293 36 proteomic proteomic JJ cord-320015-lbr2q4qh 293 37 analysis analysis NN cord-320015-lbr2q4qh 293 38 of of IN cord-320015-lbr2q4qh 293 39 invertebrate invertebrate JJ cord-320015-lbr2q4qh 293 40 iridovirus iridovirus NNP cord-320015-lbr2q4qh 293 41 type type NN cord-320015-lbr2q4qh 293 42 9 9 CD cord-320015-lbr2q4qh 293 43 Complete complete JJ cord-320015-lbr2q4qh 293 44 genome genome NN cord-320015-lbr2q4qh 293 45 sequence sequence NN cord-320015-lbr2q4qh 293 46 of of IN cord-320015-lbr2q4qh 293 47 a a DT cord-320015-lbr2q4qh 293 48 megalocytivirus megalocytivirus NN cord-320015-lbr2q4qh 293 49 ( ( -LRB- cord-320015-lbr2q4qh 293 50 family family NN cord-320015-lbr2q4qh 293 51 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 293 52 ) ) -RRB- cord-320015-lbr2q4qh 293 53 associated associate VBN cord-320015-lbr2q4qh 293 54 with with IN cord-320015-lbr2q4qh 293 55 turbot turbot NN cord-320015-lbr2q4qh 293 56 mortality mortality NN cord-320015-lbr2q4qh 293 57 in in IN cord-320015-lbr2q4qh 293 58 China China NNP cord-320015-lbr2q4qh 294 1 The the DT cord-320015-lbr2q4qh 294 2 iridoviruses iridoviruse NNS cord-320015-lbr2q4qh 294 3 Comparative Comparative NNP cord-320015-lbr2q4qh 294 4 ultrastructure ultrastructure NN cord-320015-lbr2q4qh 294 5 of of IN cord-320015-lbr2q4qh 294 6 iridoviridae iridoviridae NNP cord-320015-lbr2q4qh 295 1 The the DT cord-320015-lbr2q4qh 295 2 capsid capsid NN cord-320015-lbr2q4qh 295 3 proteins protein NNS cord-320015-lbr2q4qh 295 4 of of IN cord-320015-lbr2q4qh 295 5 a a DT cord-320015-lbr2q4qh 295 6 large large JJ cord-320015-lbr2q4qh 295 7 , , , cord-320015-lbr2q4qh 295 8 icosahedral icosahedral JJ cord-320015-lbr2q4qh 295 9 dsDNA dsdna JJ cord-320015-lbr2q4qh 295 10 virus virus NN cord-320015-lbr2q4qh 295 11 Cloning cloning NN cord-320015-lbr2q4qh 295 12 , , , cord-320015-lbr2q4qh 295 13 sequence sequence NN cord-320015-lbr2q4qh 295 14 analysis analysis NN cord-320015-lbr2q4qh 295 15 , , , cord-320015-lbr2q4qh 295 16 and and CC cord-320015-lbr2q4qh 295 17 expression expression NN cord-320015-lbr2q4qh 295 18 of of IN cord-320015-lbr2q4qh 295 19 the the DT cord-320015-lbr2q4qh 295 20 major major JJ cord-320015-lbr2q4qh 295 21 capsid capsid NN cord-320015-lbr2q4qh 295 22 protein protein NN cord-320015-lbr2q4qh 295 23 of of IN cord-320015-lbr2q4qh 295 24 the the DT cord-320015-lbr2q4qh 295 25 iridovirus iridovirus NNP cord-320015-lbr2q4qh 295 26 frog frog NNP cord-320015-lbr2q4qh 295 27 virus virus NN cord-320015-lbr2q4qh 295 28 3 3 CD cord-320015-lbr2q4qh 295 29 Molecular molecular JJ cord-320015-lbr2q4qh 295 30 characterization characterization NN cord-320015-lbr2q4qh 295 31 , , , cord-320015-lbr2q4qh 295 32 sequence sequence NN cord-320015-lbr2q4qh 295 33 analysis analysis NN cord-320015-lbr2q4qh 295 34 , , , cord-320015-lbr2q4qh 295 35 and and CC cord-320015-lbr2q4qh 295 36 taxonomic taxonomic JJ cord-320015-lbr2q4qh 295 37 position position NN cord-320015-lbr2q4qh 295 38 of of IN cord-320015-lbr2q4qh 295 39 newly newly RB cord-320015-lbr2q4qh 295 40 isolated isolate VBN cord-320015-lbr2q4qh 295 41 fish fish NN cord-320015-lbr2q4qh 295 42 iridoviruses iridoviruses NNP cord-320015-lbr2q4qh 295 43 Frog Frog NNP cord-320015-lbr2q4qh 295 44 virus virus NN cord-320015-lbr2q4qh 295 45 3 3 CD cord-320015-lbr2q4qh 295 46 ORF ORF NNP cord-320015-lbr2q4qh 295 47 53R 53r CD cord-320015-lbr2q4qh 295 48 , , , cord-320015-lbr2q4qh 295 49 a a DT cord-320015-lbr2q4qh 295 50 putative putative JJ cord-320015-lbr2q4qh 295 51 myristoylated myristoylate VBN cord-320015-lbr2q4qh 295 52 membrane membrane NN cord-320015-lbr2q4qh 295 53 protein protein NN cord-320015-lbr2q4qh 295 54 , , , cord-320015-lbr2q4qh 295 55 is be VBZ cord-320015-lbr2q4qh 295 56 essential essential JJ cord-320015-lbr2q4qh 295 57 for for IN cord-320015-lbr2q4qh 295 58 virus virus NN cord-320015-lbr2q4qh 295 59 replication replication NN cord-320015-lbr2q4qh 295 60 in in IN cord-320015-lbr2q4qh 295 61 vitro vitro FW cord-320015-lbr2q4qh 295 62 Identification identification NN cord-320015-lbr2q4qh 295 63 of of IN cord-320015-lbr2q4qh 295 64 a a DT cord-320015-lbr2q4qh 295 65 novel novel JJ cord-320015-lbr2q4qh 295 66 marine marine JJ cord-320015-lbr2q4qh 295 67 fish fish NN cord-320015-lbr2q4qh 295 68 virus virus NN cord-320015-lbr2q4qh 295 69 , , , cord-320015-lbr2q4qh 295 70 Singapore Singapore NNP cord-320015-lbr2q4qh 295 71 grouper grouper NN cord-320015-lbr2q4qh 295 72 iridovirus iridovirus NN cord-320015-lbr2q4qh 295 73 - - HYPH cord-320015-lbr2q4qh 295 74 encoded encode VBN cord-320015-lbr2q4qh 295 75 microRNAs microRNAs NNPS cord-320015-lbr2q4qh 295 76 expressed express VBN cord-320015-lbr2q4qh 295 77 in in IN cord-320015-lbr2q4qh 295 78 grouper grouper NN cord-320015-lbr2q4qh 295 79 cells cell NNS cord-320015-lbr2q4qh 295 80 by by IN cord-320015-lbr2q4qh 295 81 Solexa Solexa NNP cord-320015-lbr2q4qh 295 82 sequencing sequence VBG cord-320015-lbr2q4qh 295 83 Transcriptome Transcriptome NNP cord-320015-lbr2q4qh 295 84 analysis analysis NN cord-320015-lbr2q4qh 295 85 of of IN cord-320015-lbr2q4qh 295 86 Frog Frog NNP cord-320015-lbr2q4qh 295 87 virus virus NN cord-320015-lbr2q4qh 295 88 3 3 CD cord-320015-lbr2q4qh 295 89 , , , cord-320015-lbr2q4qh 295 90 the the DT cord-320015-lbr2q4qh 295 91 type type NN cord-320015-lbr2q4qh 295 92 species specie NNS cord-320015-lbr2q4qh 295 93 of of IN cord-320015-lbr2q4qh 295 94 the the DT cord-320015-lbr2q4qh 295 95 genus genus NN cord-320015-lbr2q4qh 295 96 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 295 97 , , , cord-320015-lbr2q4qh 295 98 family family NN cord-320015-lbr2q4qh 295 99 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 295 100 Whole Whole NNP cord-320015-lbr2q4qh 295 101 - - HYPH cord-320015-lbr2q4qh 295 102 genome genome NN cord-320015-lbr2q4qh 295 103 transcriptional transcriptional JJ cord-320015-lbr2q4qh 295 104 profiles profile NNS cord-320015-lbr2q4qh 295 105 of of IN cord-320015-lbr2q4qh 295 106 a a DT cord-320015-lbr2q4qh 295 107 novel novel JJ cord-320015-lbr2q4qh 295 108 marine marine JJ cord-320015-lbr2q4qh 295 109 fish fish NN cord-320015-lbr2q4qh 295 110 iridovirus iridovirus NN cord-320015-lbr2q4qh 295 111 , , , cord-320015-lbr2q4qh 295 112 Singapore Singapore NNP cord-320015-lbr2q4qh 295 113 grouper grouper NN cord-320015-lbr2q4qh 295 114 iridovirus iridovirus NN cord-320015-lbr2q4qh 295 115 ( ( -LRB- cord-320015-lbr2q4qh 295 116 SGIV SGIV NNP cord-320015-lbr2q4qh 295 117 ) ) -RRB- cord-320015-lbr2q4qh 295 118 in in IN cord-320015-lbr2q4qh 295 119 virus virus NN cord-320015-lbr2q4qh 295 120 - - HYPH cord-320015-lbr2q4qh 295 121 infected infect VBN cord-320015-lbr2q4qh 295 122 grouper grouper NN cord-320015-lbr2q4qh 295 123 spleen spleen NN cord-320015-lbr2q4qh 295 124 cell cell NN cord-320015-lbr2q4qh 295 125 cultures culture NNS cord-320015-lbr2q4qh 295 126 and and CC cord-320015-lbr2q4qh 295 127 in in IN cord-320015-lbr2q4qh 295 128 orange orange RB cord-320015-lbr2q4qh 295 129 - - HYPH cord-320015-lbr2q4qh 295 130 spotted spot VBN cord-320015-lbr2q4qh 295 131 grouper grouper NN cord-320015-lbr2q4qh 295 132 , , . cord-320015-lbr2q4qh 296 1 Epinephulus Epinephulus NNP cord-320015-lbr2q4qh 296 2 coioides coioide NNS cord-320015-lbr2q4qh 297 1 Temporal temporal JJ cord-320015-lbr2q4qh 297 2 and and CC cord-320015-lbr2q4qh 297 3 differential differential JJ cord-320015-lbr2q4qh 297 4 gene gene NN cord-320015-lbr2q4qh 297 5 expression expression NN cord-320015-lbr2q4qh 297 6 of of IN cord-320015-lbr2q4qh 297 7 Singapore Singapore NNP cord-320015-lbr2q4qh 297 8 grouper grouper NN cord-320015-lbr2q4qh 297 9 iridovirus iridovirus NN cord-320015-lbr2q4qh 298 1 A a DT cord-320015-lbr2q4qh 298 2 decade decade NN cord-320015-lbr2q4qh 298 3 of of IN cord-320015-lbr2q4qh 298 4 advances advance NNS cord-320015-lbr2q4qh 298 5 in in IN cord-320015-lbr2q4qh 298 6 iridovirus iridovirus NNP cord-320015-lbr2q4qh 298 7 research research NN cord-320015-lbr2q4qh 298 8 Ultrastructural Ultrastructural NNP cord-320015-lbr2q4qh 298 9 and and CC cord-320015-lbr2q4qh 298 10 biochemical biochemical JJ cord-320015-lbr2q4qh 298 11 study study NN cord-320015-lbr2q4qh 298 12 of of IN cord-320015-lbr2q4qh 298 13 frog frog NNP cord-320015-lbr2q4qh 298 14 virus virus NN cord-320015-lbr2q4qh 298 15 3 3 CD cord-320015-lbr2q4qh 298 16 uptake uptake NN cord-320015-lbr2q4qh 298 17 by by IN cord-320015-lbr2q4qh 299 1 BHK-21 bhk-21 DT cord-320015-lbr2q4qh 299 2 cells cell VBZ cord-320015-lbr2q4qh 299 3 Comparison comparison NN cord-320015-lbr2q4qh 299 4 of of IN cord-320015-lbr2q4qh 299 5 the the DT cord-320015-lbr2q4qh 299 6 properties property NNS cord-320015-lbr2q4qh 299 7 of of IN cord-320015-lbr2q4qh 299 8 enveloped envelop VBN cord-320015-lbr2q4qh 299 9 and and CC cord-320015-lbr2q4qh 299 10 naked naked JJ cord-320015-lbr2q4qh 299 11 frog frog NN cord-320015-lbr2q4qh 299 12 virus virus NN cord-320015-lbr2q4qh 299 13 3 3 CD cord-320015-lbr2q4qh 300 1 ( ( -LRB- cord-320015-lbr2q4qh 300 2 FV3 FV3 NNP cord-320015-lbr2q4qh 300 3 ) ) -RRB- cord-320015-lbr2q4qh 300 4 particles particle VBZ cord-320015-lbr2q4qh 300 5 Penetration penetration NN cord-320015-lbr2q4qh 300 6 and and CC cord-320015-lbr2q4qh 300 7 uncoating uncoating NN cord-320015-lbr2q4qh 300 8 of of IN cord-320015-lbr2q4qh 300 9 frog frog NN cord-320015-lbr2q4qh 300 10 virus virus NN cord-320015-lbr2q4qh 300 11 3 3 CD cord-320015-lbr2q4qh 300 12 ( ( -LRB- cord-320015-lbr2q4qh 300 13 FV3 FV3 NNP cord-320015-lbr2q4qh 300 14 ) ) -RRB- cord-320015-lbr2q4qh 300 15 in in IN cord-320015-lbr2q4qh 300 16 cultured cultured JJ cord-320015-lbr2q4qh 300 17 rat rat NN cord-320015-lbr2q4qh 300 18 Kupffer Kupffer NNP cord-320015-lbr2q4qh 300 19 cells cell NNS cord-320015-lbr2q4qh 300 20 Entry Entry NNP cord-320015-lbr2q4qh 300 21 of of IN cord-320015-lbr2q4qh 300 22 Tiger Tiger NNP cord-320015-lbr2q4qh 300 23 Frog Frog NNP cord-320015-lbr2q4qh 300 24 Virus Virus NNP cord-320015-lbr2q4qh 300 25 ( ( -LRB- cord-320015-lbr2q4qh 300 26 an an DT cord-320015-lbr2q4qh 300 27 Iridovirus Iridovirus NNP cord-320015-lbr2q4qh 300 28 ) ) -RRB- cord-320015-lbr2q4qh 300 29 into into IN cord-320015-lbr2q4qh 300 30 HepG2 HepG2 NNP cord-320015-lbr2q4qh 300 31 Cells Cells NNPS cord-320015-lbr2q4qh 300 32 via via IN cord-320015-lbr2q4qh 300 33 a a DT cord-320015-lbr2q4qh 300 34 pH ph NN cord-320015-lbr2q4qh 300 35 - - HYPH cord-320015-lbr2q4qh 300 36 Dependent dependent JJ cord-320015-lbr2q4qh 300 37 , , , cord-320015-lbr2q4qh 300 38 Atypical atypical JJ cord-320015-lbr2q4qh 300 39 , , , cord-320015-lbr2q4qh 300 40 Caveola Caveola NNP cord-320015-lbr2q4qh 300 41 - - HYPH cord-320015-lbr2q4qh 300 42 Mediated Mediated NNP cord-320015-lbr2q4qh 300 43 Endocytosis Endocytosis NNP cord-320015-lbr2q4qh 300 44 Pathway Pathway NNP cord-320015-lbr2q4qh 300 45 Comparative Comparative NNP cord-320015-lbr2q4qh 300 46 genomic genomic JJ cord-320015-lbr2q4qh 300 47 analyses analysis NNS cord-320015-lbr2q4qh 300 48 of of IN cord-320015-lbr2q4qh 300 49 frog frog NN cord-320015-lbr2q4qh 300 50 virus virus NN cord-320015-lbr2q4qh 300 51 3 3 CD cord-320015-lbr2q4qh 300 52 , , , cord-320015-lbr2q4qh 300 53 type type NN cord-320015-lbr2q4qh 300 54 species specie NNS cord-320015-lbr2q4qh 300 55 of of IN cord-320015-lbr2q4qh 300 56 the the DT cord-320015-lbr2q4qh 300 57 genus genus NN cord-320015-lbr2q4qh 300 58 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 300 59 ( ( -LRB- cord-320015-lbr2q4qh 300 60 family family NN cord-320015-lbr2q4qh 300 61 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 300 62 ) ) -RRB- cord-320015-lbr2q4qh 300 63 Sequence sequence NN cord-320015-lbr2q4qh 300 64 analysis analysis NN cord-320015-lbr2q4qh 300 65 of of IN cord-320015-lbr2q4qh 300 66 the the DT cord-320015-lbr2q4qh 300 67 complete complete JJ cord-320015-lbr2q4qh 300 68 genome genome NN cord-320015-lbr2q4qh 300 69 of of IN cord-320015-lbr2q4qh 300 70 an an DT cord-320015-lbr2q4qh 300 71 iridovirus iridovirus NN cord-320015-lbr2q4qh 300 72 isolated isolate VBN cord-320015-lbr2q4qh 300 73 from from IN cord-320015-lbr2q4qh 300 74 the the DT cord-320015-lbr2q4qh 300 75 tiger tiger NN cord-320015-lbr2q4qh 300 76 frog frog NNP cord-320015-lbr2q4qh 301 1 Nongenetic nongenetic JJ cord-320015-lbr2q4qh 301 2 reactivation reactivation NN cord-320015-lbr2q4qh 301 3 of of IN cord-320015-lbr2q4qh 301 4 frog frog NN cord-320015-lbr2q4qh 301 5 virus virus NN cord-320015-lbr2q4qh 301 6 3 3 CD cord-320015-lbr2q4qh 301 7 DNA DNA NNP cord-320015-lbr2q4qh 301 8 Macromolecular Macromolecular NNP cord-320015-lbr2q4qh 301 9 synthesis synthesis NN cord-320015-lbr2q4qh 301 10 in in IN cord-320015-lbr2q4qh 301 11 cells cell NNS cord-320015-lbr2q4qh 301 12 infected infect VBN cord-320015-lbr2q4qh 301 13 by by IN cord-320015-lbr2q4qh 301 14 frog frog NN cord-320015-lbr2q4qh 301 15 virus virus NN cord-320015-lbr2q4qh 301 16 3 3 CD cord-320015-lbr2q4qh 301 17 . . . cord-320015-lbr2q4qh 302 1 VIII VIII NNP cord-320015-lbr2q4qh 302 2 . . . cord-320015-lbr2q4qh 303 1 The the DT cord-320015-lbr2q4qh 303 2 nucleus nucleus NN cord-320015-lbr2q4qh 303 3 is be VBZ cord-320015-lbr2q4qh 303 4 a a DT cord-320015-lbr2q4qh 303 5 site site NN cord-320015-lbr2q4qh 303 6 of of IN cord-320015-lbr2q4qh 303 7 frog frog NN cord-320015-lbr2q4qh 303 8 virus virus NN cord-320015-lbr2q4qh 303 9 3 3 CD cord-320015-lbr2q4qh 303 10 DNA DNA NNP cord-320015-lbr2q4qh 303 11 and and CC cord-320015-lbr2q4qh 303 12 RNA RNA NNP cord-320015-lbr2q4qh 303 13 synthesis synthesis NN cord-320015-lbr2q4qh 303 14 Frog Frog NNP cord-320015-lbr2q4qh 303 15 virus virus NN cord-320015-lbr2q4qh 303 16 3 3 CD cord-320015-lbr2q4qh 303 17 requires require VBZ cord-320015-lbr2q4qh 303 18 RNA RNA NNP cord-320015-lbr2q4qh 303 19 polymerase polymerase NN cord-320015-lbr2q4qh 303 20 II II NNP cord-320015-lbr2q4qh 303 21 for for IN cord-320015-lbr2q4qh 303 22 its -PRON- PRP$ cord-320015-lbr2q4qh 303 23 replication replication NN cord-320015-lbr2q4qh 303 24 Transactivation Transactivation NNP cord-320015-lbr2q4qh 303 25 of of IN cord-320015-lbr2q4qh 303 26 an an DT cord-320015-lbr2q4qh 303 27 immediate immediate JJ cord-320015-lbr2q4qh 303 28 - - HYPH cord-320015-lbr2q4qh 303 29 early early JJ cord-320015-lbr2q4qh 303 30 frog frog NN cord-320015-lbr2q4qh 303 31 virus virus NN cord-320015-lbr2q4qh 303 32 3 3 CD cord-320015-lbr2q4qh 303 33 promoter promoter NN cord-320015-lbr2q4qh 303 34 by by IN cord-320015-lbr2q4qh 303 35 a a DT cord-320015-lbr2q4qh 303 36 virion virion NN cord-320015-lbr2q4qh 303 37 protein protein NN cord-320015-lbr2q4qh 303 38 Frog Frog NNP cord-320015-lbr2q4qh 303 39 virus virus NN cord-320015-lbr2q4qh 304 1 3 3 CD cord-320015-lbr2q4qh 304 2 DNA dna NN cord-320015-lbr2q4qh 304 3 replication replication NN cord-320015-lbr2q4qh 304 4 occurs occur VBZ cord-320015-lbr2q4qh 304 5 in in IN cord-320015-lbr2q4qh 304 6 two two CD cord-320015-lbr2q4qh 304 7 stages stage NNS cord-320015-lbr2q4qh 305 1 The the DT cord-320015-lbr2q4qh 305 2 role role NN cord-320015-lbr2q4qh 305 3 of of IN cord-320015-lbr2q4qh 305 4 DNA dna NN cord-320015-lbr2q4qh 305 5 methylation methylation NN cord-320015-lbr2q4qh 305 6 in in IN cord-320015-lbr2q4qh 305 7 virus virus NN cord-320015-lbr2q4qh 305 8 replication replication NN cord-320015-lbr2q4qh 305 9 : : : cord-320015-lbr2q4qh 305 10 inhibition inhibition NN cord-320015-lbr2q4qh 305 11 of of IN cord-320015-lbr2q4qh 305 12 frog frog NN cord-320015-lbr2q4qh 305 13 virus virus NN cord-320015-lbr2q4qh 305 14 3 3 CD cord-320015-lbr2q4qh 305 15 replication replication NN cord-320015-lbr2q4qh 305 16 by by IN cord-320015-lbr2q4qh 305 17 5-azacytidine 5-azacytidine CD cord-320015-lbr2q4qh 305 18 DNA dna NN cord-320015-lbr2q4qh 305 19 methyltransferase methyltransferase NN cord-320015-lbr2q4qh 305 20 induced induce VBN cord-320015-lbr2q4qh 305 21 by by IN cord-320015-lbr2q4qh 305 22 frog frog NN cord-320015-lbr2q4qh 305 23 virus virus NN cord-320015-lbr2q4qh 305 24 3 3 CD cord-320015-lbr2q4qh 305 25 Interaction Interaction NNP cord-320015-lbr2q4qh 305 26 of of IN cord-320015-lbr2q4qh 305 27 frog frog NN cord-320015-lbr2q4qh 305 28 virus virus NN cord-320015-lbr2q4qh 305 29 3 3 CD cord-320015-lbr2q4qh 305 30 with with IN cord-320015-lbr2q4qh 305 31 the the DT cord-320015-lbr2q4qh 305 32 cytomatrix cytomatrix NNP cord-320015-lbr2q4qh 305 33 . . . cord-320015-lbr2q4qh 306 1 II II NNP cord-320015-lbr2q4qh 306 2 . . . cord-320015-lbr2q4qh 307 1 Structure structure NN cord-320015-lbr2q4qh 307 2 and and CC cord-320015-lbr2q4qh 307 3 composition composition NN cord-320015-lbr2q4qh 307 4 of of IN cord-320015-lbr2q4qh 307 5 the the DT cord-320015-lbr2q4qh 307 6 virus virus NN cord-320015-lbr2q4qh 307 7 assembly assembly NN cord-320015-lbr2q4qh 307 8 site site NN cord-320015-lbr2q4qh 308 1 An an DT cord-320015-lbr2q4qh 308 2 unusual unusual JJ cord-320015-lbr2q4qh 308 3 replication replication NN cord-320015-lbr2q4qh 308 4 strategy strategy NN cord-320015-lbr2q4qh 308 5 of of IN cord-320015-lbr2q4qh 308 6 an an DT cord-320015-lbr2q4qh 308 7 animal animal NN cord-320015-lbr2q4qh 308 8 iridovirus iridovirus NN cord-320015-lbr2q4qh 309 1 African african JJ cord-320015-lbr2q4qh 309 2 swine swine NN cord-320015-lbr2q4qh 309 3 fever fever NN cord-320015-lbr2q4qh 309 4 virus virus NN cord-320015-lbr2q4qh 309 5 polyprotein polyprotein NNP cord-320015-lbr2q4qh 309 6 pp62 pp62 NNP cord-320015-lbr2q4qh 309 7 is be VBZ cord-320015-lbr2q4qh 309 8 essential essential JJ cord-320015-lbr2q4qh 309 9 for for IN cord-320015-lbr2q4qh 309 10 viral viral JJ cord-320015-lbr2q4qh 309 11 core core NN cord-320015-lbr2q4qh 309 12 development development NN cord-320015-lbr2q4qh 309 13 African african JJ cord-320015-lbr2q4qh 309 14 swine swine NN cord-320015-lbr2q4qh 309 15 fever fever NN cord-320015-lbr2q4qh 309 16 virus virus NN cord-320015-lbr2q4qh 309 17 polyproteins polyprotein VBZ cord-320015-lbr2q4qh 309 18 pp220 pp220 NNP cord-320015-lbr2q4qh 309 19 and and CC cord-320015-lbr2q4qh 310 1 pp62 pp62 NNP cord-320015-lbr2q4qh 310 2 assemble assemble VB cord-320015-lbr2q4qh 310 3 into into IN cord-320015-lbr2q4qh 310 4 the the DT cord-320015-lbr2q4qh 310 5 core core NN cord-320015-lbr2q4qh 310 6 shell shell NN cord-320015-lbr2q4qh 311 1 The the DT cord-320015-lbr2q4qh 311 2 genome genome NN cord-320015-lbr2q4qh 311 3 of of IN cord-320015-lbr2q4qh 311 4 frog frog NN cord-320015-lbr2q4qh 311 5 virus virus NN cord-320015-lbr2q4qh 311 6 3 3 CD cord-320015-lbr2q4qh 311 7 , , , cord-320015-lbr2q4qh 311 8 an an DT cord-320015-lbr2q4qh 311 9 animal animal NN cord-320015-lbr2q4qh 311 10 DNA dna NN cord-320015-lbr2q4qh 311 11 virus virus NN cord-320015-lbr2q4qh 311 12 , , , cord-320015-lbr2q4qh 311 13 is be VBZ cord-320015-lbr2q4qh 311 14 circularly circularly RB cord-320015-lbr2q4qh 311 15 permuted permute VBN cord-320015-lbr2q4qh 311 16 and and CC cord-320015-lbr2q4qh 311 17 terminally terminally RB cord-320015-lbr2q4qh 311 18 redundant redundant JJ cord-320015-lbr2q4qh 311 19 Tryptophan tryptophan NN cord-320015-lbr2q4qh 311 20 residues residue NNS cord-320015-lbr2q4qh 311 21 in in IN cord-320015-lbr2q4qh 311 22 the the DT cord-320015-lbr2q4qh 311 23 portal portal JJ cord-320015-lbr2q4qh 311 24 protein protein NN cord-320015-lbr2q4qh 311 25 of of IN cord-320015-lbr2q4qh 311 26 herpes herpes NNP cord-320015-lbr2q4qh 311 27 simplex simplex NNP cord-320015-lbr2q4qh 311 28 virus virus NN cord-320015-lbr2q4qh 311 29 1 1 CD cord-320015-lbr2q4qh 311 30 critical critical JJ cord-320015-lbr2q4qh 311 31 to to IN cord-320015-lbr2q4qh 311 32 the the DT cord-320015-lbr2q4qh 311 33 interaction interaction NN cord-320015-lbr2q4qh 311 34 with with IN cord-320015-lbr2q4qh 311 35 scafford scafford NNP cord-320015-lbr2q4qh 311 36 protreins protrein NNS cord-320015-lbr2q4qh 311 37 and and CC cord-320015-lbr2q4qh 311 38 incorporation incorporation NN cord-320015-lbr2q4qh 311 39 of of IN cord-320015-lbr2q4qh 311 40 the the DT cord-320015-lbr2q4qh 311 41 portal portal NN cord-320015-lbr2q4qh 311 42 into into IN cord-320015-lbr2q4qh 311 43 capsids capsid NNS cord-320015-lbr2q4qh 311 44 Electron Electron NNP cord-320015-lbr2q4qh 311 45 cryotomography cryotomography NN cord-320015-lbr2q4qh 311 46 reveals reveal VBZ cord-320015-lbr2q4qh 311 47 the the DT cord-320015-lbr2q4qh 311 48 portal portal NN cord-320015-lbr2q4qh 311 49 in in IN cord-320015-lbr2q4qh 311 50 the the DT cord-320015-lbr2q4qh 311 51 herpesvirus herpesvirus NN cord-320015-lbr2q4qh 311 52 capsid capsid NN cord-320015-lbr2q4qh 311 53 Distinct Distinct NNP cord-320015-lbr2q4qh 311 54 DNA dna NN cord-320015-lbr2q4qh 311 55 exit exit NN cord-320015-lbr2q4qh 311 56 and and CC cord-320015-lbr2q4qh 311 57 packaging packaging NN cord-320015-lbr2q4qh 311 58 portals portal NNS cord-320015-lbr2q4qh 311 59 in in IN cord-320015-lbr2q4qh 311 60 the the DT cord-320015-lbr2q4qh 311 61 virus virus NN cord-320015-lbr2q4qh 311 62 Acanthamoeba Acanthamoeba NNP cord-320015-lbr2q4qh 311 63 polyphaga polyphaga NNP cord-320015-lbr2q4qh 311 64 mimivirus mimivirus NN cord-320015-lbr2q4qh 311 65 Temperature temperature NN cord-320015-lbr2q4qh 311 66 - - HYPH cord-320015-lbr2q4qh 311 67 sensitive sensitive JJ cord-320015-lbr2q4qh 311 68 mutants mutant NNS cord-320015-lbr2q4qh 311 69 of of IN cord-320015-lbr2q4qh 311 70 frog frog NN cord-320015-lbr2q4qh 311 71 virus virus NN cord-320015-lbr2q4qh 311 72 3 3 CD cord-320015-lbr2q4qh 311 73 : : : cord-320015-lbr2q4qh 311 74 biochemical biochemical JJ cord-320015-lbr2q4qh 311 75 and and CC cord-320015-lbr2q4qh 311 76 genetic genetic JJ cord-320015-lbr2q4qh 311 77 characterization characterization NN cord-320015-lbr2q4qh 311 78 Viral viral JJ cord-320015-lbr2q4qh 311 79 envelope envelope NN cord-320015-lbr2q4qh 311 80 protein protein NN cord-320015-lbr2q4qh 311 81 53R 53r CD cord-320015-lbr2q4qh 311 82 gene gene NN cord-320015-lbr2q4qh 311 83 highly highly RB cord-320015-lbr2q4qh 311 84 specific specific JJ cord-320015-lbr2q4qh 311 85 silencing silencing NN cord-320015-lbr2q4qh 311 86 and and CC cord-320015-lbr2q4qh 311 87 iridovirus iridovirus NN cord-320015-lbr2q4qh 311 88 resistance resistance NN cord-320015-lbr2q4qh 311 89 in in IN cord-320015-lbr2q4qh 311 90 fish fish NN cord-320015-lbr2q4qh 311 91 cells cell NNS cord-320015-lbr2q4qh 311 92 by by IN cord-320015-lbr2q4qh 311 93 AmiRNA AmiRNA JJR cord-320015-lbr2q4qh 311 94 Identification identification NN cord-320015-lbr2q4qh 311 95 and and CC cord-320015-lbr2q4qh 311 96 characterization characterization NN cord-320015-lbr2q4qh 311 97 of of IN cord-320015-lbr2q4qh 311 98 a a DT cord-320015-lbr2q4qh 311 99 novel novel JJ cord-320015-lbr2q4qh 311 100 envelope envelope NN cord-320015-lbr2q4qh 311 101 protein protein NN cord-320015-lbr2q4qh 311 102 in in IN cord-320015-lbr2q4qh 311 103 Rana Rana NNP cord-320015-lbr2q4qh 311 104 grylio grylio NNP cord-320015-lbr2q4qh 311 105 virus virus NN cord-320015-lbr2q4qh 311 106 Isolation isolation NN cord-320015-lbr2q4qh 311 107 and and CC cord-320015-lbr2q4qh 311 108 characterization characterization NN cord-320015-lbr2q4qh 311 109 of of IN cord-320015-lbr2q4qh 311 110 a a DT cord-320015-lbr2q4qh 311 111 frog frog NN cord-320015-lbr2q4qh 311 112 virus virus NN cord-320015-lbr2q4qh 311 113 3 3 CD cord-320015-lbr2q4qh 311 114 variant variant NN cord-320015-lbr2q4qh 311 115 resistant resistant JJ cord-320015-lbr2q4qh 311 116 to to IN cord-320015-lbr2q4qh 311 117 phosphonoacetate phosphonoacetate VB cord-320015-lbr2q4qh 311 118 : : : cord-320015-lbr2q4qh 311 119 genetic genetic JJ cord-320015-lbr2q4qh 311 120 evidence evidence NN cord-320015-lbr2q4qh 311 121 for for IN cord-320015-lbr2q4qh 311 122 a a DT cord-320015-lbr2q4qh 311 123 virus virus NN cord-320015-lbr2q4qh 311 124 - - HYPH cord-320015-lbr2q4qh 311 125 specific specific JJ cord-320015-lbr2q4qh 311 126 DNA dna NN cord-320015-lbr2q4qh 311 127 polymerase polymerase NN cord-320015-lbr2q4qh 311 128 Characterization characterization NN cord-320015-lbr2q4qh 311 129 of of IN cord-320015-lbr2q4qh 311 130 an an DT cord-320015-lbr2q4qh 311 131 early early JJ cord-320015-lbr2q4qh 311 132 gene gene NN cord-320015-lbr2q4qh 311 133 encoding encoding NN cord-320015-lbr2q4qh 311 134 for for IN cord-320015-lbr2q4qh 311 135 dUTPase dUTPase NNP cord-320015-lbr2q4qh 311 136 in in IN cord-320015-lbr2q4qh 311 137 Rana Rana NNP cord-320015-lbr2q4qh 311 138 grylio grylio NNP cord-320015-lbr2q4qh 311 139 virus virus NN cord-320015-lbr2q4qh 311 140 Inhibition Inhibition NNP cord-320015-lbr2q4qh 311 141 of of IN cord-320015-lbr2q4qh 311 142 iridovirus iridovirus NNP cord-320015-lbr2q4qh 311 143 protein protein NN cord-320015-lbr2q4qh 311 144 synthesis synthesis NN cord-320015-lbr2q4qh 311 145 and and CC cord-320015-lbr2q4qh 311 146 virus virus NN cord-320015-lbr2q4qh 311 147 replication replication NN cord-320015-lbr2q4qh 311 148 by by IN cord-320015-lbr2q4qh 311 149 antisense antisense NNP cord-320015-lbr2q4qh 311 150 morpholino morpholino NNP cord-320015-lbr2q4qh 311 151 oligonucleotides oligonucleotide NNS cord-320015-lbr2q4qh 311 152 targeted target VBN cord-320015-lbr2q4qh 311 153 to to IN cord-320015-lbr2q4qh 311 154 the the DT cord-320015-lbr2q4qh 311 155 major major JJ cord-320015-lbr2q4qh 311 156 capsid capsid NN cord-320015-lbr2q4qh 311 157 protein protein NN cord-320015-lbr2q4qh 311 158 , , , cord-320015-lbr2q4qh 311 159 the the DT cord-320015-lbr2q4qh 311 160 18 18 CD cord-320015-lbr2q4qh 311 161 kDa kDa NNPS cord-320015-lbr2q4qh 311 162 immediate immediate JJ cord-320015-lbr2q4qh 311 163 - - HYPH cord-320015-lbr2q4qh 311 164 early early JJ cord-320015-lbr2q4qh 311 165 protein protein NN cord-320015-lbr2q4qh 311 166 , , , cord-320015-lbr2q4qh 311 167 and and CC cord-320015-lbr2q4qh 311 168 a a DT cord-320015-lbr2q4qh 311 169 viral viral JJ cord-320015-lbr2q4qh 311 170 homolog homolog NN cord-320015-lbr2q4qh 311 171 of of IN cord-320015-lbr2q4qh 311 172 RNA RNA NNP cord-320015-lbr2q4qh 311 173 polymerase polymerase NN cord-320015-lbr2q4qh 311 174 II II NNP cord-320015-lbr2q4qh 312 1 The the DT cord-320015-lbr2q4qh 312 2 structure structure NN cord-320015-lbr2q4qh 312 3 of of IN cord-320015-lbr2q4qh 312 4 G4 G4 NNP cord-320015-lbr2q4qh 312 5 , , , cord-320015-lbr2q4qh 312 6 the the DT cord-320015-lbr2q4qh 312 7 poxvirus poxvirus NNP cord-320015-lbr2q4qh 312 8 disulfide disulfide NNP cord-320015-lbr2q4qh 312 9 oxidoreductase oxidoreductase NN cord-320015-lbr2q4qh 312 10 essential essential NN cord-320015-lbr2q4qh 312 11 for for IN cord-320015-lbr2q4qh 312 12 virus virus NN cord-320015-lbr2q4qh 312 13 maturation maturation NN cord-320015-lbr2q4qh 312 14 and and CC cord-320015-lbr2q4qh 312 15 infectivity infectivity NN cord-320015-lbr2q4qh 312 16 Ecology ecology NN cord-320015-lbr2q4qh 312 17 of of IN cord-320015-lbr2q4qh 312 18 viruses virus NNS cord-320015-lbr2q4qh 312 19 infecting infect VBG cord-320015-lbr2q4qh 312 20 ectothermic ectothermic JJ cord-320015-lbr2q4qh 312 21 vertebrates vertebrate NNS cord-320015-lbr2q4qh 312 22 -the -the DT cord-320015-lbr2q4qh 312 23 impact impact NN cord-320015-lbr2q4qh 312 24 of of IN cord-320015-lbr2q4qh 312 25 ranavirus ranavirus NNP cord-320015-lbr2q4qh 312 26 infections infection NNS cord-320015-lbr2q4qh 312 27 on on IN cord-320015-lbr2q4qh 312 28 amphibians amphibian NNS cord-320015-lbr2q4qh 312 29 Macromolecular Macromolecular NNP cord-320015-lbr2q4qh 312 30 synthesis synthesis NN cord-320015-lbr2q4qh 312 31 in in IN cord-320015-lbr2q4qh 312 32 cells cell NNS cord-320015-lbr2q4qh 312 33 infected infect VBN cord-320015-lbr2q4qh 312 34 by by IN cord-320015-lbr2q4qh 312 35 frog frog NN cord-320015-lbr2q4qh 312 36 virus virus NN cord-320015-lbr2q4qh 312 37 3 3 CD cord-320015-lbr2q4qh 312 38 . . . cord-320015-lbr2q4qh 313 1 X. X. NNP cord-320015-lbr2q4qh 313 2 Inhibition inhibition NN cord-320015-lbr2q4qh 313 3 of of IN cord-320015-lbr2q4qh 313 4 cellular cellular JJ cord-320015-lbr2q4qh 313 5 protein protein NN cord-320015-lbr2q4qh 313 6 synthesis synthesis NN cord-320015-lbr2q4qh 313 7 by by IN cord-320015-lbr2q4qh 313 8 heat heat NN cord-320015-lbr2q4qh 313 9 - - HYPH cord-320015-lbr2q4qh 313 10 inactivated inactivate VBN cord-320015-lbr2q4qh 313 11 virus virus NN cord-320015-lbr2q4qh 313 12 Frog Frog NNP cord-320015-lbr2q4qh 313 13 virus virus NN cord-320015-lbr2q4qh 314 1 3-induced 3-induced CD cord-320015-lbr2q4qh 314 2 translational translational JJ cord-320015-lbr2q4qh 314 3 shut shut NN cord-320015-lbr2q4qh 314 4 - - HYPH cord-320015-lbr2q4qh 314 5 off off NN cord-320015-lbr2q4qh 314 6 : : : cord-320015-lbr2q4qh 314 7 activation activation NN cord-320015-lbr2q4qh 314 8 of of IN cord-320015-lbr2q4qh 314 9 an an DT cord-320015-lbr2q4qh 314 10 eIF-2 eIF-2 NNP cord-320015-lbr2q4qh 314 11 kinase kinase NN cord-320015-lbr2q4qh 314 12 in in IN cord-320015-lbr2q4qh 314 13 virus virus NN cord-320015-lbr2q4qh 314 14 - - HYPH cord-320015-lbr2q4qh 314 15 infected infect VBN cord-320015-lbr2q4qh 314 16 cells cell NNS cord-320015-lbr2q4qh 314 17 Frog Frog NNP cord-320015-lbr2q4qh 314 18 virus virus NN cord-320015-lbr2q4qh 314 19 3-mediated 3-mediated CD cord-320015-lbr2q4qh 314 20 translational translational JJ cord-320015-lbr2q4qh 314 21 shut shut NN cord-320015-lbr2q4qh 314 22 - - HYPH cord-320015-lbr2q4qh 314 23 off off NN cord-320015-lbr2q4qh 314 24 : : : cord-320015-lbr2q4qh 315 1 frog frog NNP cord-320015-lbr2q4qh 315 2 virus virus NN cord-320015-lbr2q4qh 315 3 3 3 CD cord-320015-lbr2q4qh 315 4 messages message NNS cord-320015-lbr2q4qh 315 5 are be VBP cord-320015-lbr2q4qh 315 6 translationally translationally RB cord-320015-lbr2q4qh 315 7 more more RBR cord-320015-lbr2q4qh 315 8 efficient efficient JJ cord-320015-lbr2q4qh 315 9 than than IN cord-320015-lbr2q4qh 315 10 host host NN cord-320015-lbr2q4qh 315 11 and and CC cord-320015-lbr2q4qh 315 12 heterologous heterologous JJ cord-320015-lbr2q4qh 315 13 viral viral JJ cord-320015-lbr2q4qh 315 14 messages message NNS cord-320015-lbr2q4qh 315 15 under under IN cord-320015-lbr2q4qh 315 16 conditions condition NNS cord-320015-lbr2q4qh 315 17 of of IN cord-320015-lbr2q4qh 315 18 increased increase VBN cord-320015-lbr2q4qh 315 19 translational translational JJ cord-320015-lbr2q4qh 315 20 stress stress NN cord-320015-lbr2q4qh 315 21 Induction Induction NNP cord-320015-lbr2q4qh 315 22 of of IN cord-320015-lbr2q4qh 315 23 apoptosis apoptosis NN cord-320015-lbr2q4qh 315 24 in in IN cord-320015-lbr2q4qh 315 25 frog frog NN cord-320015-lbr2q4qh 315 26 virus virus NN cord-320015-lbr2q4qh 315 27 3-infected 3-infected CD cord-320015-lbr2q4qh 315 28 cells cell NNS cord-320015-lbr2q4qh 315 29 Heat heat NN cord-320015-lbr2q4qh 315 30 - - HYPH cord-320015-lbr2q4qh 315 31 inactivated inactivate VBN cord-320015-lbr2q4qh 315 32 frog frog NN cord-320015-lbr2q4qh 315 33 virus virus NN cord-320015-lbr2q4qh 315 34 3 3 CD cord-320015-lbr2q4qh 315 35 selectively selectively RB cord-320015-lbr2q4qh 315 36 inhibits inhibit VBZ cord-320015-lbr2q4qh 315 37 equine equine JJ cord-320015-lbr2q4qh 315 38 herpesvirus herpesvirus NN cord-320015-lbr2q4qh 315 39 type type NN cord-320015-lbr2q4qh 315 40 1 1 CD cord-320015-lbr2q4qh 315 41 translation translation NN cord-320015-lbr2q4qh 315 42 in in IN cord-320015-lbr2q4qh 315 43 a a DT cord-320015-lbr2q4qh 315 44 temporal temporal JJ cord-320015-lbr2q4qh 315 45 class class NN cord-320015-lbr2q4qh 315 46 - - HYPH cord-320015-lbr2q4qh 315 47 dependent dependent JJ cord-320015-lbr2q4qh 315 48 manner manner NN cord-320015-lbr2q4qh 315 49 Translational translational JJ cord-320015-lbr2q4qh 315 50 efficiency efficiency NN cord-320015-lbr2q4qh 315 51 : : : cord-320015-lbr2q4qh 315 52 iridovirus iridovirus NNP cord-320015-lbr2q4qh 315 53 early early RB cord-320015-lbr2q4qh 315 54 mRNAs mrnas PRP cord-320015-lbr2q4qh 315 55 outcompete outcompete VBP cord-320015-lbr2q4qh 315 56 tobacco tobacco NN cord-320015-lbr2q4qh 315 57 mosaic mosaic JJ cord-320015-lbr2q4qh 315 58 virus virus NN cord-320015-lbr2q4qh 315 59 message message NN cord-320015-lbr2q4qh 315 60 in in IN cord-320015-lbr2q4qh 315 61 vitro vitro FW cord-320015-lbr2q4qh 315 62 Characterization characterization NN cord-320015-lbr2q4qh 315 63 of of IN cord-320015-lbr2q4qh 315 64 a a DT cord-320015-lbr2q4qh 315 65 ranavirus ranavirus NNP cord-320015-lbr2q4qh 315 66 inhibitor inhibitor NN cord-320015-lbr2q4qh 315 67 of of IN cord-320015-lbr2q4qh 315 68 the the DT cord-320015-lbr2q4qh 315 69 antiviral antiviral JJ cord-320015-lbr2q4qh 315 70 protein protein NN cord-320015-lbr2q4qh 315 71 kinase kinase VBZ cord-320015-lbr2q4qh 315 72 PKR PKR NNP cord-320015-lbr2q4qh 316 1 The the DT cord-320015-lbr2q4qh 316 2 role role NN cord-320015-lbr2q4qh 316 3 of of IN cord-320015-lbr2q4qh 316 4 the the DT cord-320015-lbr2q4qh 316 5 PKR PKR NNP cord-320015-lbr2q4qh 316 6 - - HYPH cord-320015-lbr2q4qh 316 7 inhibitory inhibitory JJ cord-320015-lbr2q4qh 316 8 genes gene NNS cord-320015-lbr2q4qh 316 9 , , , cord-320015-lbr2q4qh 316 10 E3L e3l NN cord-320015-lbr2q4qh 316 11 and and CC cord-320015-lbr2q4qh 316 12 K3L k3l CD cord-320015-lbr2q4qh 316 13 , , , cord-320015-lbr2q4qh 316 14 in in IN cord-320015-lbr2q4qh 316 15 determining determine VBG cord-320015-lbr2q4qh 316 16 vaccinia vaccinia NN cord-320015-lbr2q4qh 316 17 virus virus NN cord-320015-lbr2q4qh 316 18 host host NN cord-320015-lbr2q4qh 316 19 range range NN cord-320015-lbr2q4qh 316 20 Rana Rana NNP cord-320015-lbr2q4qh 316 21 catesbeiana catesbeiana NNP cord-320015-lbr2q4qh 317 1 virus virus NN cord-320015-lbr2q4qh 317 2 Z Z NNP cord-320015-lbr2q4qh 317 3 ( ( -LRB- cord-320015-lbr2q4qh 317 4 RCV RCV NNP cord-320015-lbr2q4qh 317 5 - - HYPH cord-320015-lbr2q4qh 317 6 Z Z NNP cord-320015-lbr2q4qh 317 7 ) ) -RRB- cord-320015-lbr2q4qh 317 8 : : : cord-320015-lbr2q4qh 318 1 a a DT cord-320015-lbr2q4qh 318 2 novel novel JJ cord-320015-lbr2q4qh 318 3 pathogenic pathogenic JJ cord-320015-lbr2q4qh 318 4 ranavirus ranavirus NN cord-320015-lbr2q4qh 318 5 Comparison comparison NN cord-320015-lbr2q4qh 318 6 of of IN cord-320015-lbr2q4qh 318 7 the the DT cord-320015-lbr2q4qh 318 8 eIF-2alpha eif-2alpha JJ cord-320015-lbr2q4qh 318 9 homologous homologous JJ cord-320015-lbr2q4qh 318 10 proteins protein NNS cord-320015-lbr2q4qh 318 11 of of IN cord-320015-lbr2q4qh 318 12 seven seven CD cord-320015-lbr2q4qh 318 13 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 318 14 ( ( -LRB- cord-320015-lbr2q4qh 318 15 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 318 16 ) ) -RRB- cord-320015-lbr2q4qh 318 17 Steroid steroid JJ cord-320015-lbr2q4qh 318 18 hormone hormone NN cord-320015-lbr2q4qh 318 19 synthesis synthesis NN cord-320015-lbr2q4qh 318 20 by by IN cord-320015-lbr2q4qh 318 21 vaccinia vaccinia NN cord-320015-lbr2q4qh 318 22 virus virus NN cord-320015-lbr2q4qh 318 23 suppresses suppress VBZ cord-320015-lbr2q4qh 318 24 the the DT cord-320015-lbr2q4qh 318 25 inflammatory inflammatory JJ cord-320015-lbr2q4qh 318 26 response response NN cord-320015-lbr2q4qh 318 27 to to IN cord-320015-lbr2q4qh 318 28 infection infection NN cord-320015-lbr2q4qh 318 29 Steroid steroid JJ cord-320015-lbr2q4qh 318 30 hormone hormone NN cord-320015-lbr2q4qh 318 31 synthesis synthesis NN cord-320015-lbr2q4qh 318 32 by by IN cord-320015-lbr2q4qh 318 33 a a DT cord-320015-lbr2q4qh 318 34 vaccinia vaccinia NN cord-320015-lbr2q4qh 318 35 enzyme enzyme NN cord-320015-lbr2q4qh 318 36 : : : cord-320015-lbr2q4qh 318 37 a a DT cord-320015-lbr2q4qh 318 38 new new JJ cord-320015-lbr2q4qh 318 39 type type NN cord-320015-lbr2q4qh 318 40 of of IN cord-320015-lbr2q4qh 318 41 virus virus NN cord-320015-lbr2q4qh 318 42 virulence virulence NN cord-320015-lbr2q4qh 318 43 factor factor NN cord-320015-lbr2q4qh 318 44 Proapoptotic Proapoptotic NNP cord-320015-lbr2q4qh 318 45 signaling signal VBG cord-320015-lbr2q4qh 318 46 induced induce VBN cord-320015-lbr2q4qh 318 47 by by IN cord-320015-lbr2q4qh 318 48 RIG rig NN cord-320015-lbr2q4qh 318 49 - - : cord-320015-lbr2q4qh 318 50 I I NNP cord-320015-lbr2q4qh 318 51 and and CC cord-320015-lbr2q4qh 318 52 MDA-5 MDA-5 NNP cord-320015-lbr2q4qh 318 53 results result NNS cord-320015-lbr2q4qh 318 54 in in IN cord-320015-lbr2q4qh 318 55 type type NN cord-320015-lbr2q4qh 319 1 I -PRON- PRP cord-320015-lbr2q4qh 320 1 interferon interferon NN cord-320015-lbr2q4qh 320 2 - - HYPH cord-320015-lbr2q4qh 320 3 independent independent JJ cord-320015-lbr2q4qh 320 4 apoptosis apoptosis NN cord-320015-lbr2q4qh 320 5 in in IN cord-320015-lbr2q4qh 320 6 human human JJ cord-320015-lbr2q4qh 320 7 melanoma melanoma NN cord-320015-lbr2q4qh 320 8 cells cell NNS cord-320015-lbr2q4qh 320 9 CARD card NN cord-320015-lbr2q4qh 320 10 games game NNS cord-320015-lbr2q4qh 320 11 in in IN cord-320015-lbr2q4qh 320 12 apoptosis apoptosis NN cord-320015-lbr2q4qh 320 13 and and CC cord-320015-lbr2q4qh 320 14 immunity immunity NN cord-320015-lbr2q4qh 321 1 The the DT cord-320015-lbr2q4qh 321 2 CARD card NN cord-320015-lbr2q4qh 321 3 domain domain NN cord-320015-lbr2q4qh 321 4 : : : cord-320015-lbr2q4qh 321 5 a a DT cord-320015-lbr2q4qh 321 6 new new JJ cord-320015-lbr2q4qh 321 7 apoptotic apoptotic JJ cord-320015-lbr2q4qh 321 8 signalling signal VBG cord-320015-lbr2q4qh 321 9 motif motif NN cord-320015-lbr2q4qh 322 1 MAVS MAVS NNP cord-320015-lbr2q4qh 322 2 - - HYPH cord-320015-lbr2q4qh 322 3 mediated mediate VBN cord-320015-lbr2q4qh 322 4 apoptosis apoptosis NN cord-320015-lbr2q4qh 322 5 and and CC cord-320015-lbr2q4qh 322 6 its -PRON- PRP$ cord-320015-lbr2q4qh 322 7 inhibition inhibition NN cord-320015-lbr2q4qh 322 8 by by IN cord-320015-lbr2q4qh 322 9 viral viral JJ cord-320015-lbr2q4qh 322 10 proteins protein NNS cord-320015-lbr2q4qh 323 1 The the DT cord-320015-lbr2q4qh 323 2 RNA RNA NNP cord-320015-lbr2q4qh 323 3 helicase helicase NN cord-320015-lbr2q4qh 323 4 RIG rig NN cord-320015-lbr2q4qh 323 5 - - : cord-320015-lbr2q4qh 323 6 I -PRON- PRP cord-320015-lbr2q4qh 323 7 has have VBZ cord-320015-lbr2q4qh 323 8 an an DT cord-320015-lbr2q4qh 323 9 essential essential JJ cord-320015-lbr2q4qh 323 10 function function NN cord-320015-lbr2q4qh 323 11 in in IN cord-320015-lbr2q4qh 323 12 double double RB cord-320015-lbr2q4qh 323 13 - - HYPH cord-320015-lbr2q4qh 323 14 stranded strand VBN cord-320015-lbr2q4qh 323 15 RNAinduced rnainduced NN cord-320015-lbr2q4qh 323 16 innate innate JJ cord-320015-lbr2q4qh 323 17 antiviral antiviral JJ cord-320015-lbr2q4qh 323 18 responses response NNS cord-320015-lbr2q4qh 323 19 RIG rig NN cord-320015-lbr2q4qh 323 20 - - : cord-320015-lbr2q4qh 323 21 I i NN cord-320015-lbr2q4qh 323 22 and and CC cord-320015-lbr2q4qh 323 23 dsRNA dsrna RB cord-320015-lbr2q4qh 323 24 - - HYPH cord-320015-lbr2q4qh 323 25 induced induce VBN cord-320015-lbr2q4qh 323 26 IFNbeta IFNbeta NNP cord-320015-lbr2q4qh 323 27 activation activation NN cord-320015-lbr2q4qh 323 28 RIGging rig VBG cord-320015-lbr2q4qh 323 29 an an DT cord-320015-lbr2q4qh 323 30 antiviral antiviral JJ cord-320015-lbr2q4qh 323 31 defense- defense- NN cord-320015-lbr2q4qh 324 1 It -PRON- PRP cord-320015-lbr2q4qh 324 2 's be VBZ cord-320015-lbr2q4qh 324 3 in in IN cord-320015-lbr2q4qh 324 4 the the DT cord-320015-lbr2q4qh 324 5 CARDs card NNS cord-320015-lbr2q4qh 324 6 Cardif Cardif NNP cord-320015-lbr2q4qh 324 7 is be VBZ cord-320015-lbr2q4qh 324 8 an an DT cord-320015-lbr2q4qh 324 9 adaptor adaptor NN cord-320015-lbr2q4qh 324 10 protein protein NN cord-320015-lbr2q4qh 324 11 in in IN cord-320015-lbr2q4qh 324 12 the the DT cord-320015-lbr2q4qh 324 13 RIG rig NN cord-320015-lbr2q4qh 324 14 - - : cord-320015-lbr2q4qh 324 15 I I NNP cord-320015-lbr2q4qh 324 16 antiviral antiviral JJ cord-320015-lbr2q4qh 324 17 pathway pathway NN cord-320015-lbr2q4qh 324 18 and and CC cord-320015-lbr2q4qh 324 19 is be VBZ cord-320015-lbr2q4qh 324 20 targeted target VBN cord-320015-lbr2q4qh 324 21 by by IN cord-320015-lbr2q4qh 324 22 hepatitis hepatitis NN cord-320015-lbr2q4qh 324 23 C C NNP cord-320015-lbr2q4qh 324 24 virus virus NN cord-320015-lbr2q4qh 324 25 Functional functional JJ cord-320015-lbr2q4qh 324 26 characterization characterization NN cord-320015-lbr2q4qh 324 27 of of IN cord-320015-lbr2q4qh 324 28 the the DT cord-320015-lbr2q4qh 324 29 RNase RNase NNP cord-320015-lbr2q4qh 324 30 III III NNP cord-320015-lbr2q4qh 324 31 gene gene NN cord-320015-lbr2q4qh 324 32 of of IN cord-320015-lbr2q4qh 324 33 rock rock NN cord-320015-lbr2q4qh 324 34 bream bream NN cord-320015-lbr2q4qh 324 35 iridovirus iridovirus NNP cord-320015-lbr2q4qh 324 36 Poxvirus Poxvirus NNP cord-320015-lbr2q4qh 324 37 immunomodulatory immunomodulatory JJ cord-320015-lbr2q4qh 324 38 strategies strategy NNS cord-320015-lbr2q4qh 324 39 : : : cord-320015-lbr2q4qh 324 40 Current current JJ cord-320015-lbr2q4qh 324 41 perspectives perspective NNS cord-320015-lbr2q4qh 324 42 Poxviruses Poxviruses NNPS cord-320015-lbr2q4qh 324 43 and and CC cord-320015-lbr2q4qh 324 44 immune immune JJ cord-320015-lbr2q4qh 324 45 evasion evasion NN cord-320015-lbr2q4qh 324 46 Identification identification NN cord-320015-lbr2q4qh 324 47 of of IN cord-320015-lbr2q4qh 324 48 host host NN cord-320015-lbr2q4qh 324 49 range range NN cord-320015-lbr2q4qh 324 50 mutants mutant NNS cord-320015-lbr2q4qh 324 51 of of IN cord-320015-lbr2q4qh 324 52 myxoma myxoma NN cord-320015-lbr2q4qh 324 53 virus virus NN cord-320015-lbr2q4qh 324 54 with with IN cord-320015-lbr2q4qh 324 55 altered altered JJ cord-320015-lbr2q4qh 324 56 oncolytic oncolytic JJ cord-320015-lbr2q4qh 324 57 potential potential NN cord-320015-lbr2q4qh 324 58 in in IN cord-320015-lbr2q4qh 324 59 human human JJ cord-320015-lbr2q4qh 324 60 glioma glioma NN cord-320015-lbr2q4qh 324 61 cells cell NNS cord-320015-lbr2q4qh 324 62 Role role NN cord-320015-lbr2q4qh 324 63 of of IN cord-320015-lbr2q4qh 324 64 viral viral JJ cord-320015-lbr2q4qh 324 65 ribonucleotide ribonucleotide NN cord-320015-lbr2q4qh 324 66 reductase reductase NN cord-320015-lbr2q4qh 324 67 in in IN cord-320015-lbr2q4qh 324 68 the the DT cord-320015-lbr2q4qh 324 69 increase increase NN cord-320015-lbr2q4qh 324 70 of of IN cord-320015-lbr2q4qh 324 71 dTTP dttp JJ cord-320015-lbr2q4qh 324 72 pool pool NN cord-320015-lbr2q4qh 324 73 size size NN cord-320015-lbr2q4qh 324 74 in in IN cord-320015-lbr2q4qh 325 1 herpes herpes NNP cord-320015-lbr2q4qh 325 2 simplex simplex NNP cord-320015-lbr2q4qh 325 3 virus virus NN cord-320015-lbr2q4qh 325 4 - - HYPH cord-320015-lbr2q4qh 325 5 infected infect VBN cord-320015-lbr2q4qh 325 6 Vero Vero NNP cord-320015-lbr2q4qh 325 7 cells cell NNS cord-320015-lbr2q4qh 325 8 Ribonucleotide Ribonucleotide NNP cord-320015-lbr2q4qh 325 9 reductase reductase NN cord-320015-lbr2q4qh 325 10 - - HYPH cord-320015-lbr2q4qh 325 11 deficient deficient JJ cord-320015-lbr2q4qh 325 12 mutants mutant NNS cord-320015-lbr2q4qh 325 13 of of IN cord-320015-lbr2q4qh 325 14 pseudorabies pseudorabies NN cord-320015-lbr2q4qh 325 15 virus virus NN cord-320015-lbr2q4qh 325 16 are be VBP cord-320015-lbr2q4qh 325 17 avirulent avirulent JJ cord-320015-lbr2q4qh 325 18 for for IN cord-320015-lbr2q4qh 325 19 pigs pig NNS cord-320015-lbr2q4qh 325 20 and and CC cord-320015-lbr2q4qh 325 21 induce induce VB cord-320015-lbr2q4qh 325 22 partial partial JJ cord-320015-lbr2q4qh 325 23 protective protective JJ cord-320015-lbr2q4qh 325 24 immunity immunity NN cord-320015-lbr2q4qh 326 1 African african JJ cord-320015-lbr2q4qh 326 2 swine swine NN cord-320015-lbr2q4qh 326 3 fever fever NN cord-320015-lbr2q4qh 326 4 virus virus NN cord-320015-lbr2q4qh 326 5 dUTPase dUTPase '' cord-320015-lbr2q4qh 326 6 is be VBZ cord-320015-lbr2q4qh 326 7 a a DT cord-320015-lbr2q4qh 326 8 highly highly RB cord-320015-lbr2q4qh 326 9 specific specific JJ cord-320015-lbr2q4qh 326 10 enzyme enzyme NN cord-320015-lbr2q4qh 326 11 required require VBN cord-320015-lbr2q4qh 326 12 for for IN cord-320015-lbr2q4qh 326 13 efficient efficient JJ cord-320015-lbr2q4qh 326 14 replication replication NN cord-320015-lbr2q4qh 326 15 in in IN cord-320015-lbr2q4qh 326 16 swine swine NN cord-320015-lbr2q4qh 326 17 macrophages macrophage NNS cord-320015-lbr2q4qh 326 18 Identification identification NN cord-320015-lbr2q4qh 326 19 and and CC cord-320015-lbr2q4qh 326 20 characterization characterization NN cord-320015-lbr2q4qh 326 21 of of IN cord-320015-lbr2q4qh 326 22 a a DT cord-320015-lbr2q4qh 326 23 novel novel JJ cord-320015-lbr2q4qh 326 24 gene gene NN cord-320015-lbr2q4qh 326 25 of of IN cord-320015-lbr2q4qh 326 26 grouper grouper NNP cord-320015-lbr2q4qh 326 27 iridovirus iridovirus NNP cord-320015-lbr2q4qh 326 28 encoding encode VBG cord-320015-lbr2q4qh 327 1 a a DT cord-320015-lbr2q4qh 327 2 purine purine NN cord-320015-lbr2q4qh 327 3 nucleoside nucleoside NN cord-320015-lbr2q4qh 327 4 phosphorylase phosphorylase NNP cord-320015-lbr2q4qh 327 5 Genomic genomic JJ cord-320015-lbr2q4qh 327 6 sequence sequence NN cord-320015-lbr2q4qh 327 7 of of IN cord-320015-lbr2q4qh 327 8 a a DT cord-320015-lbr2q4qh 327 9 ranavirus ranavirus NN cord-320015-lbr2q4qh 327 10 ( ( -LRB- cord-320015-lbr2q4qh 327 11 family family NN cord-320015-lbr2q4qh 327 12 Iridoviridae Iridoviridae NNP cord-320015-lbr2q4qh 327 13 ) ) -RRB- cord-320015-lbr2q4qh 327 14 associated associate VBN cord-320015-lbr2q4qh 327 15 with with IN cord-320015-lbr2q4qh 327 16 salamander salamander NN cord-320015-lbr2q4qh 327 17 mortalities mortality NNS cord-320015-lbr2q4qh 327 18 in in IN cord-320015-lbr2q4qh 327 19 North North NNP cord-320015-lbr2q4qh 327 20 America America NNP cord-320015-lbr2q4qh 327 21 Poxviruses Poxviruses NNPS cord-320015-lbr2q4qh 327 22 : : : cord-320015-lbr2q4qh 327 23 past past JJ cord-320015-lbr2q4qh 327 24 , , , cord-320015-lbr2q4qh 327 25 present present JJ cord-320015-lbr2q4qh 327 26 , , , cord-320015-lbr2q4qh 327 27 and and CC cord-320015-lbr2q4qh 327 28 future future NN cord-320015-lbr2q4qh 327 29 How how WRB cord-320015-lbr2q4qh 327 30 similar similar JJ cord-320015-lbr2q4qh 327 31 are be VBP cord-320015-lbr2q4qh 327 32 poxviruses poxvirus NNS cord-320015-lbr2q4qh 327 33 ? ? . cord-320015-lbr2q4qh 328 1 Science Science NNP cord-320015-lbr2q4qh 328 2 Host Host NNP cord-320015-lbr2q4qh 328 3 range range NN cord-320015-lbr2q4qh 328 4 , , , cord-320015-lbr2q4qh 328 5 host host NN cord-320015-lbr2q4qh 328 6 specificity specificity NN cord-320015-lbr2q4qh 328 7 and and CC cord-320015-lbr2q4qh 328 8 hypothesized hypothesize VBD cord-320015-lbr2q4qh 328 9 host host NN cord-320015-lbr2q4qh 328 10 shift shift NN cord-320015-lbr2q4qh 328 11 events event NNS cord-320015-lbr2q4qh 328 12 among among IN cord-320015-lbr2q4qh 328 13 viruses virus NNS cord-320015-lbr2q4qh 328 14 of of IN cord-320015-lbr2q4qh 328 15 lower low JJR cord-320015-lbr2q4qh 328 16 vertebrates vertebrate NNS cord-320015-lbr2q4qh 329 1 Experimental experimental JJ cord-320015-lbr2q4qh 329 2 evidence evidence NN cord-320015-lbr2q4qh 329 3 that that IN cord-320015-lbr2q4qh 329 4 amphibian amphibian NN cord-320015-lbr2q4qh 329 5 ranaviruses ranaviruse NNS cord-320015-lbr2q4qh 329 6 are be VBP cord-320015-lbr2q4qh 329 7 multihost multihost NN cord-320015-lbr2q4qh 329 8 pathogens pathogen NNS cord-320015-lbr2q4qh 329 9 Molecular Molecular NNP cord-320015-lbr2q4qh 329 10 Evolutionary Evolutionary NNP cord-320015-lbr2q4qh 329 11 Genetics Genetics NNP cord-320015-lbr2q4qh 329 12 Analysis Analysis NNP cord-320015-lbr2q4qh 329 13 ( ( -LRB- cord-320015-lbr2q4qh 329 14 MEGA MEGA NNP cord-320015-lbr2q4qh 329 15 ) ) -RRB- cord-320015-lbr2q4qh 329 16 software software NN cord-320015-lbr2q4qh 329 17 version version NN cord-320015-lbr2q4qh 329 18 4.0 4.0 CD cord-320015-lbr2q4qh 329 19 Antiviral Antiviral NNP cord-320015-lbr2q4qh 329 20 Immunity Immunity NNP cord-320015-lbr2q4qh 329 21 in in IN cord-320015-lbr2q4qh 329 22 Amphibians amphibian NNS cord-320015-lbr2q4qh 329 23 . . . cord-320015-lbr2q4qh 330 1 Viruses virus NNS cord-320015-lbr2q4qh 330 2 , , , cord-320015-lbr2q4qh 330 3 submitted submit VBN cord-320015-lbr2q4qh 330 4 for for IN cord-320015-lbr2q4qh 330 5 publication publication NN cord-320015-lbr2q4qh 330 6 Ecopathology Ecopathology NNP cord-320015-lbr2q4qh 330 7 of of IN cord-320015-lbr2q4qh 330 8 Ranaviruses Ranaviruses NNPS cord-320015-lbr2q4qh 330 9 Infecting infect VBG cord-320015-lbr2q4qh 330 10 Amphibians Amphibians NNPS cord-320015-lbr2q4qh 330 11 . . . cord-320015-lbr2q4qh 331 1 Viruses virus NNS cord-320015-lbr2q4qh 331 2 , , , cord-320015-lbr2q4qh 331 3 submitted submit VBN cord-320015-lbr2q4qh 331 4 for for IN cord-320015-lbr2q4qh 331 5 publication publication NN cord-320015-lbr2q4qh 331 6 Emerging emerge VBG cord-320015-lbr2q4qh 331 7 viral viral JJ cord-320015-lbr2q4qh 331 8 diseases disease NNS cord-320015-lbr2q4qh 331 9 of of IN cord-320015-lbr2q4qh 331 10 fish fish NN cord-320015-lbr2q4qh 331 11 and and CC cord-320015-lbr2q4qh 331 12 shrimp shrimp NN cord-320015-lbr2q4qh 331 13 Iridovirus Iridovirus NNP cord-320015-lbr2q4qh 331 14 infections infection NNS cord-320015-lbr2q4qh 331 15 in in IN cord-320015-lbr2q4qh 331 16 finfish finfish NN cord-320015-lbr2q4qh 331 17 - - HYPH cord-320015-lbr2q4qh 331 18 Critical critical JJ cord-320015-lbr2q4qh 331 19 review review NN cord-320015-lbr2q4qh 331 20 with with IN cord-320015-lbr2q4qh 331 21 emphasis emphasis NN cord-320015-lbr2q4qh 331 22 on on IN cord-320015-lbr2q4qh 331 23 ranaviruses ranaviruses NNP cord-320015-lbr2q4qh 331 24 Mass Mass NNP cord-320015-lbr2q4qh 331 25 mortality mortality NN cord-320015-lbr2q4qh 331 26 associated associate VBN cord-320015-lbr2q4qh 331 27 with with IN cord-320015-lbr2q4qh 331 28 a a DT cord-320015-lbr2q4qh 331 29 frog frog NN cord-320015-lbr2q4qh 331 30 virus virus NN cord-320015-lbr2q4qh 331 31 3-like 3-like CD cord-320015-lbr2q4qh 331 32 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 331 33 infection infection NN cord-320015-lbr2q4qh 331 34 in in IN cord-320015-lbr2q4qh 331 35 farmed farm VBN cord-320015-lbr2q4qh 331 36 tadpoles tadpole NNS cord-320015-lbr2q4qh 331 37 Rana Rana NNP cord-320015-lbr2q4qh 331 38 catesbeiana catesbeiana NNP cord-320015-lbr2q4qh 331 39 from from IN cord-320015-lbr2q4qh 331 40 Brazil Brazil NNP cord-320015-lbr2q4qh 331 41 Ecology Ecology NNP cord-320015-lbr2q4qh 331 42 and and CC cord-320015-lbr2q4qh 331 43 pathology pathology NN cord-320015-lbr2q4qh 331 44 of of IN cord-320015-lbr2q4qh 331 45 amphibian amphibian NN cord-320015-lbr2q4qh 331 46 ranaviruses ranaviruses NNP cord-320015-lbr2q4qh 331 47 Development Development NNP cord-320015-lbr2q4qh 331 48 and and CC cord-320015-lbr2q4qh 331 49 characterization characterization NN cord-320015-lbr2q4qh 331 50 of of IN cord-320015-lbr2q4qh 331 51 a a DT cord-320015-lbr2q4qh 331 52 model model NN cord-320015-lbr2q4qh 331 53 system system NN cord-320015-lbr2q4qh 331 54 to to TO cord-320015-lbr2q4qh 331 55 study study VB cord-320015-lbr2q4qh 331 56 amphibian amphibian NN cord-320015-lbr2q4qh 331 57 immune immune JJ cord-320015-lbr2q4qh 331 58 responses response NNS cord-320015-lbr2q4qh 331 59 to to IN cord-320015-lbr2q4qh 331 60 iridoviruses iridoviruses NNP cord-320015-lbr2q4qh 331 61 Generation Generation NNP cord-320015-lbr2q4qh 331 62 of of IN cord-320015-lbr2q4qh 331 63 a a DT cord-320015-lbr2q4qh 331 64 long long RB cord-320015-lbr2q4qh 331 65 - - HYPH cord-320015-lbr2q4qh 331 66 lasting last VBG cord-320015-lbr2q4qh 331 67 , , , cord-320015-lbr2q4qh 331 68 protective protective JJ cord-320015-lbr2q4qh 331 69 , , , cord-320015-lbr2q4qh 331 70 and and CC cord-320015-lbr2q4qh 331 71 neutralizing neutralize VBG cord-320015-lbr2q4qh 331 72 antibody antibody NN cord-320015-lbr2q4qh 331 73 response response NN cord-320015-lbr2q4qh 331 74 to to IN cord-320015-lbr2q4qh 331 75 the the DT cord-320015-lbr2q4qh 331 76 ranavirus ranavirus NNP cord-320015-lbr2q4qh 331 77 FV3 FV3 NNP cord-320015-lbr2q4qh 331 78 by by IN cord-320015-lbr2q4qh 331 79 the the DT cord-320015-lbr2q4qh 331 80 frog frog NN cord-320015-lbr2q4qh 332 1 Xenopus Xenopus NNP cord-320015-lbr2q4qh 332 2 Innate innate JJ cord-320015-lbr2q4qh 332 3 immune immune JJ cord-320015-lbr2q4qh 332 4 responses response NNS cord-320015-lbr2q4qh 332 5 and and CC cord-320015-lbr2q4qh 332 6 permissiveness permissiveness NN cord-320015-lbr2q4qh 332 7 to to IN cord-320015-lbr2q4qh 332 8 ranavirus ranavirus NN cord-320015-lbr2q4qh 332 9 infection infection NN cord-320015-lbr2q4qh 332 10 of of IN cord-320015-lbr2q4qh 332 11 peritoneal peritoneal JJ cord-320015-lbr2q4qh 332 12 leukocytes leukocyte NNS cord-320015-lbr2q4qh 332 13 in in IN cord-320015-lbr2q4qh 332 14 the the DT cord-320015-lbr2q4qh 332 15 frog frog NN cord-320015-lbr2q4qh 333 1 Xenopus Xenopus NNP cord-320015-lbr2q4qh 333 2 laevis laevi NNS cord-320015-lbr2q4qh 334 1 Characterization characterization NN cord-320015-lbr2q4qh 334 2 of of IN cord-320015-lbr2q4qh 334 3 primary primary JJ cord-320015-lbr2q4qh 334 4 and and CC cord-320015-lbr2q4qh 334 5 memory memory NN cord-320015-lbr2q4qh 334 6 CD8 CD8 NNP cord-320015-lbr2q4qh 334 7 T T NNP cord-320015-lbr2q4qh 334 8 - - HYPH cord-320015-lbr2q4qh 334 9 cell cell NN cord-320015-lbr2q4qh 334 10 responses response NNS cord-320015-lbr2q4qh 334 11 against against IN cord-320015-lbr2q4qh 334 12 ranavirus ranavirus NNP cord-320015-lbr2q4qh 334 13 ( ( -LRB- cord-320015-lbr2q4qh 334 14 FV3 FV3 NNP cord-320015-lbr2q4qh 334 15 ) ) -RRB- cord-320015-lbr2q4qh 334 16 in in IN cord-320015-lbr2q4qh 334 17 Xenopus Xenopus NNP cord-320015-lbr2q4qh 334 18 laevis laevi VBZ cord-320015-lbr2q4qh 334 19 Xenopus Xenopus NNP cord-320015-lbr2q4qh 334 20 laevis laevi NNS cord-320015-lbr2q4qh 334 21 : : : cord-320015-lbr2q4qh 334 22 A a DT cord-320015-lbr2q4qh 334 23 possible possible JJ cord-320015-lbr2q4qh 334 24 vector vector NN cord-320015-lbr2q4qh 334 25 of of IN cord-320015-lbr2q4qh 334 26 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 334 27 infection infection NN cord-320015-lbr2q4qh 334 28 ? ? . cord-320015-lbr2q4qh 335 1 Waterborne waterborne JJ cord-320015-lbr2q4qh 335 2 infectivity infectivity NN cord-320015-lbr2q4qh 335 3 of of IN cord-320015-lbr2q4qh 335 4 the the DT cord-320015-lbr2q4qh 335 5 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 335 6 frog frog NN cord-320015-lbr2q4qh 335 7 virus virus NN cord-320015-lbr2q4qh 335 8 3 3 CD cord-320015-lbr2q4qh 335 9 in in IN cord-320015-lbr2q4qh 335 10 Xenopus Xenopus NNP cord-320015-lbr2q4qh 335 11 laevis laevi NNS cord-320015-lbr2q4qh 336 1 Adaptive adaptive JJ cord-320015-lbr2q4qh 336 2 immunity immunity NN cord-320015-lbr2q4qh 336 3 and and CC cord-320015-lbr2q4qh 336 4 histopathology histopathology NN cord-320015-lbr2q4qh 336 5 in in IN cord-320015-lbr2q4qh 336 6 frog frog NN cord-320015-lbr2q4qh 336 7 virus virus NN cord-320015-lbr2q4qh 336 8 3-infected 3-infected CD cord-320015-lbr2q4qh 336 9 Xenopus Xenopus NNP cord-320015-lbr2q4qh 336 10 Immunogenicity Immunogenicity NNP cord-320015-lbr2q4qh 336 11 , , , cord-320015-lbr2q4qh 336 12 retention retention NN cord-320015-lbr2q4qh 336 13 and and CC cord-320015-lbr2q4qh 336 14 protective protective JJ cord-320015-lbr2q4qh 336 15 effects effect NNS cord-320015-lbr2q4qh 336 16 of of IN cord-320015-lbr2q4qh 336 17 the the DT cord-320015-lbr2q4qh 336 18 protein protein NN cord-320015-lbr2q4qh 336 19 derivatives derivative NNS cord-320015-lbr2q4qh 336 20 of of IN cord-320015-lbr2q4qh 336 21 formalin formalin NN cord-320015-lbr2q4qh 336 22 - - HYPH cord-320015-lbr2q4qh 336 23 inactivated inactivate VBN cord-320015-lbr2q4qh 336 24 red red JJ cord-320015-lbr2q4qh 336 25 seabream seabream NN cord-320015-lbr2q4qh 336 26 iridovirus iridovirus NNP cord-320015-lbr2q4qh 336 27 ( ( -LRB- cord-320015-lbr2q4qh 336 28 RSIV RSIV NNP cord-320015-lbr2q4qh 336 29 ) ) -RRB- cord-320015-lbr2q4qh 336 30 vaccine vaccine NN cord-320015-lbr2q4qh 336 31 in in IN cord-320015-lbr2q4qh 336 32 red red JJ cord-320015-lbr2q4qh 336 33 seabream seabream NN cord-320015-lbr2q4qh 336 34 , , , cord-320015-lbr2q4qh 336 35 Pagrus Pagrus NNP cord-320015-lbr2q4qh 336 36 major major JJ cord-320015-lbr2q4qh 336 37 Genetic genetic JJ cord-320015-lbr2q4qh 336 38 vaccines vaccine NNS cord-320015-lbr2q4qh 336 39 protect protect VBP cord-320015-lbr2q4qh 336 40 red red JJ cord-320015-lbr2q4qh 336 41 seabream seabream NN cord-320015-lbr2q4qh 336 42 , , , cord-320015-lbr2q4qh 336 43 Pagrus Pagrus NNP cord-320015-lbr2q4qh 336 44 major major NN cord-320015-lbr2q4qh 336 45 , , , cord-320015-lbr2q4qh 336 46 upon upon IN cord-320015-lbr2q4qh 336 47 challenge challenge NN cord-320015-lbr2q4qh 336 48 with with IN cord-320015-lbr2q4qh 336 49 red red NNP cord-320015-lbr2q4qh 336 50 seabream seabream NNP cord-320015-lbr2q4qh 336 51 iridovirus iridovirus NNP cord-320015-lbr2q4qh 336 52 ( ( -LRB- cord-320015-lbr2q4qh 336 53 RSIV RSIV NNP cord-320015-lbr2q4qh 336 54 ) ) -RRB- cord-320015-lbr2q4qh 337 1 Epizootiology epizootiology NN cord-320015-lbr2q4qh 337 2 of of IN cord-320015-lbr2q4qh 337 3 sixty sixty CD cord-320015-lbr2q4qh 337 4 - - HYPH cord-320015-lbr2q4qh 337 5 four four CD cord-320015-lbr2q4qh 337 6 amphibian amphibian NN cord-320015-lbr2q4qh 337 7 morbidity morbidity NN cord-320015-lbr2q4qh 337 8 and and CC cord-320015-lbr2q4qh 337 9 mortality mortality NN cord-320015-lbr2q4qh 337 10 events event NNS cord-320015-lbr2q4qh 337 11 in in IN cord-320015-lbr2q4qh 337 12 the the DT cord-320015-lbr2q4qh 337 13 USA USA NNP cord-320015-lbr2q4qh 337 14 Experimental Experimental NNP cord-320015-lbr2q4qh 337 15 challenge challenge NN cord-320015-lbr2q4qh 337 16 and and CC cord-320015-lbr2q4qh 337 17 clinical clinical JJ cord-320015-lbr2q4qh 337 18 cases case NNS cord-320015-lbr2q4qh 337 19 of of IN cord-320015-lbr2q4qh 337 20 Bohle Bohle NNP cord-320015-lbr2q4qh 337 21 iridovirus iridovirus NN cord-320015-lbr2q4qh 337 22 ( ( -LRB- cord-320015-lbr2q4qh 337 23 BIV BIV NNP cord-320015-lbr2q4qh 337 24 ) ) -RRB- cord-320015-lbr2q4qh 337 25 in in IN cord-320015-lbr2q4qh 337 26 native native JJ cord-320015-lbr2q4qh 337 27 Australian australian JJ cord-320015-lbr2q4qh 337 28 anurans anuran NNS cord-320015-lbr2q4qh 337 29 Molecular molecular JJ cord-320015-lbr2q4qh 337 30 characterization characterization NN cord-320015-lbr2q4qh 337 31 of of IN cord-320015-lbr2q4qh 337 32 iridoviruses iridoviruse NNS cord-320015-lbr2q4qh 337 33 isolated isolate VBN cord-320015-lbr2q4qh 337 34 from from IN cord-320015-lbr2q4qh 337 35 sympatric sympatric JJ cord-320015-lbr2q4qh 337 36 amphibians amphibian NNS cord-320015-lbr2q4qh 337 37 and and CC cord-320015-lbr2q4qh 337 38 fish fish VB cord-320015-lbr2q4qh 337 39 Susceptibility susceptibility NN cord-320015-lbr2q4qh 337 40 of of IN cord-320015-lbr2q4qh 337 41 pike pike NN cord-320015-lbr2q4qh 337 42 Esox Esox NNP cord-320015-lbr2q4qh 337 43 lucius lucius NNP cord-320015-lbr2q4qh 337 44 to to IN cord-320015-lbr2q4qh 337 45 a a DT cord-320015-lbr2q4qh 337 46 panel panel NN cord-320015-lbr2q4qh 337 47 of of IN cord-320015-lbr2q4qh 337 48 Ranavirus Ranavirus NNP cord-320015-lbr2q4qh 337 49 isolates isolate VBZ cord-320015-lbr2q4qh 337 50 Experimental experimental JJ cord-320015-lbr2q4qh 337 51 demonstration demonstration NN cord-320015-lbr2q4qh 337 52 of of IN cord-320015-lbr2q4qh 337 53 pathogenicity pathogenicity NN cord-320015-lbr2q4qh 337 54 of of IN cord-320015-lbr2q4qh 337 55 a a DT cord-320015-lbr2q4qh 337 56 frog frog NN cord-320015-lbr2q4qh 337 57 virus virus NN cord-320015-lbr2q4qh 337 58 , , , cord-320015-lbr2q4qh 337 59 Bohle Bohle NNP cord-320015-lbr2q4qh 337 60 iridovirus iridovirus NNP cord-320015-lbr2q4qh 337 61 , , , cord-320015-lbr2q4qh 337 62 for for IN cord-320015-lbr2q4qh 337 63 a a DT cord-320015-lbr2q4qh 337 64 fish fish NN cord-320015-lbr2q4qh 337 65 species species NN cord-320015-lbr2q4qh 337 66 , , , cord-320015-lbr2q4qh 337 67 barramundi barramundi NN cord-320015-lbr2q4qh 337 68 Lates Lates NNP cord-320015-lbr2q4qh 337 69 Calcarifer Calcarifer NNP cord-320015-lbr2q4qh 337 70 Macromolecular Macromolecular NNP cord-320015-lbr2q4qh 337 71 synthesis synthesis NN cord-320015-lbr2q4qh 337 72 in in IN cord-320015-lbr2q4qh 337 73 cells cell NNS cord-320015-lbr2q4qh 337 74 infected infect VBN cord-320015-lbr2q4qh 337 75 by by IN cord-320015-lbr2q4qh 337 76 frog frog NN cord-320015-lbr2q4qh 337 77 virus virus NN cord-320015-lbr2q4qh 337 78 3 3 CD cord-320015-lbr2q4qh 337 79 Icosahedral icosahedral JJ cord-320015-lbr2q4qh 337 80 cytoplasmic cytoplasmic JJ cord-320015-lbr2q4qh 337 81 deoxyriboviruses deoxyriboviruse NNS cord-320015-lbr2q4qh 337 82 Viruses virus NNS cord-320015-lbr2q4qh 337 83 and and CC cord-320015-lbr2q4qh 337 84 renal renal JJ cord-320015-lbr2q4qh 337 85 carcinoma carcinoma NN cord-320015-lbr2q4qh 337 86 of of IN cord-320015-lbr2q4qh 337 87 Rana Rana NNP cord-320015-lbr2q4qh 337 88 pipiens pipien NNS cord-320015-lbr2q4qh 337 89 . . . cord-320015-lbr2q4qh 338 1 XI XI NNP cord-320015-lbr2q4qh 338 2 . . . cord-320015-lbr2q4qh 339 1 Isolation isolation NN cord-320015-lbr2q4qh 339 2 of of IN cord-320015-lbr2q4qh 339 3 frog frog NN cord-320015-lbr2q4qh 339 4 virus virus NN cord-320015-lbr2q4qh 339 5 3 3 CD cord-320015-lbr2q4qh 339 6 temperature temperature NN cord-320015-lbr2q4qh 339 7 - - HYPH cord-320015-lbr2q4qh 339 8 sensitive sensitive JJ cord-320015-lbr2q4qh 339 9 mutants mutant NNS cord-320015-lbr2q4qh 339 10 ; ; : cord-320015-lbr2q4qh 339 11 complementation complementation NN cord-320015-lbr2q4qh 339 12 and and CC cord-320015-lbr2q4qh 339 13 genetic genetic JJ cord-320015-lbr2q4qh 339 14 recombination recombination NN cord-320015-lbr2q4qh 339 15 Viruses virus NNS cord-320015-lbr2q4qh 339 16 and and CC cord-320015-lbr2q4qh 339 17 renal renal JJ cord-320015-lbr2q4qh 339 18 carcinoma carcinoma NN cord-320015-lbr2q4qh 339 19 of of IN cord-320015-lbr2q4qh 339 20 Rana Rana NNP cord-320015-lbr2q4qh 339 21 pipiens pipien NNS cord-320015-lbr2q4qh 339 22 . . . cord-320015-lbr2q4qh 340 1 XIV XIV NNP cord-320015-lbr2q4qh 340 2 . . . cord-320015-lbr2q4qh 341 1 Temperature temperature NN cord-320015-lbr2q4qh 341 2 - - HYPH cord-320015-lbr2q4qh 341 3 sensitive sensitive JJ cord-320015-lbr2q4qh 341 4 mutants mutant NNS cord-320015-lbr2q4qh 341 5 of of IN cord-320015-lbr2q4qh 341 6 frog frog NN cord-320015-lbr2q4qh 341 7 virus virus NN cord-320015-lbr2q4qh 341 8 3 3 CD cord-320015-lbr2q4qh 341 9 with with IN cord-320015-lbr2q4qh 341 10 defective defective JJ cord-320015-lbr2q4qh 341 11 encapsidation encapsidation NN cord-320015-lbr2q4qh 341 12 Characterization characterization NN cord-320015-lbr2q4qh 341 13 of of IN cord-320015-lbr2q4qh 341 14 a a DT cord-320015-lbr2q4qh 341 15 temperature temperature NN cord-320015-lbr2q4qh 341 16 - - HYPH cord-320015-lbr2q4qh 341 17 sensitive sensitive JJ cord-320015-lbr2q4qh 341 18 mutant mutant NN cord-320015-lbr2q4qh 341 19 of of IN cord-320015-lbr2q4qh 341 20 frog frog NN cord-320015-lbr2q4qh 341 21 virus virus NN cord-320015-lbr2q4qh 341 22 3 3 CD cord-320015-lbr2q4qh 341 23 defective defective JJ cord-320015-lbr2q4qh 341 24 in in IN cord-320015-lbr2q4qh 341 25 DNA dna NN cord-320015-lbr2q4qh 341 26 replication replication NN cord-320015-lbr2q4qh 341 27 Elucidation elucidation NN cord-320015-lbr2q4qh 341 28 of of IN cord-320015-lbr2q4qh 341 29 Frog Frog NNP cord-320015-lbr2q4qh 341 30 Virus Virus NNP cord-320015-lbr2q4qh 341 31 3 3 CD cord-320015-lbr2q4qh 341 32 Gene Gene NNP cord-320015-lbr2q4qh 341 33 Function Function NNP cord-320015-lbr2q4qh 341 34 and and CC cord-320015-lbr2q4qh 341 35 Pathways Pathways NNPS cord-320015-lbr2q4qh 341 36 of of IN cord-320015-lbr2q4qh 341 37 Virion Virion NNP cord-320015-lbr2q4qh 341 38 Formation Formation NNP cord-320015-lbr2q4qh 341 39 Early early JJ cord-320015-lbr2q4qh 341 40 proteins protein NNS cord-320015-lbr2q4qh 341 41 are be VBP cord-320015-lbr2q4qh 341 42 required require VBN cord-320015-lbr2q4qh 341 43 for for IN cord-320015-lbr2q4qh 341 44 the the DT cord-320015-lbr2q4qh 341 45 formation formation NN cord-320015-lbr2q4qh 341 46 of of IN cord-320015-lbr2q4qh 341 47 frog frog NN cord-320015-lbr2q4qh 341 48 virus virus NN cord-320015-lbr2q4qh 341 49 3 3 CD cord-320015-lbr2q4qh 341 50 assembly assembly NN cord-320015-lbr2q4qh 341 51 sites site NNS cord-320015-lbr2q4qh 341 52 Oligonucleotide Oligonucleotide NNP cord-320015-lbr2q4qh 341 53 - - HYPH cord-320015-lbr2q4qh 341 54 based base VBN cord-320015-lbr2q4qh 341 55 strategies strategy NNS cord-320015-lbr2q4qh 341 56 to to TO cord-320015-lbr2q4qh 341 57 reduce reduce VB cord-320015-lbr2q4qh 341 58 gene gene NN cord-320015-lbr2q4qh 341 59 expression expression NN cord-320015-lbr2q4qh 341 60 Reverse Reverse NNP cord-320015-lbr2q4qh 341 61 genetics genetic NNS cord-320015-lbr2q4qh 341 62 in in IN cord-320015-lbr2q4qh 341 63 eukaryotes eukaryote NNS cord-320015-lbr2q4qh 341 64 Antisense Antisense NNP cord-320015-lbr2q4qh 341 65 morpholino morpholino NN cord-320015-lbr2q4qh 341 66 - - HYPH cord-320015-lbr2q4qh 341 67 oligomers oligomer NNS cord-320015-lbr2q4qh 341 68 directed direct VBN cord-320015-lbr2q4qh 341 69 against against IN cord-320015-lbr2q4qh 341 70 the the DT cord-320015-lbr2q4qh 341 71 5 5 CD cord-320015-lbr2q4qh 341 72 ' ' NN cord-320015-lbr2q4qh 341 73 end end NN cord-320015-lbr2q4qh 341 74 of of IN cord-320015-lbr2q4qh 342 1 the the DT cord-320015-lbr2q4qh 342 2 genome genome NN cord-320015-lbr2q4qh 342 3 inhibit inhibit NN cord-320015-lbr2q4qh 343 1 coronavirus coronavirus NN cord-320015-lbr2q4qh 343 2 proliferation proliferation NN cord-320015-lbr2q4qh 343 3 and and CC cord-320015-lbr2q4qh 343 4 growth growth NN cord-320015-lbr2q4qh 343 5 Inhibition inhibition NN cord-320015-lbr2q4qh 343 6 of of IN cord-320015-lbr2q4qh 343 7 flavivirus flavivirus JJ cord-320015-lbr2q4qh 343 8 infections infection NNS cord-320015-lbr2q4qh 343 9 by by IN cord-320015-lbr2q4qh 343 10 antisense antisense NN cord-320015-lbr2q4qh 343 11 oligomers oligomer NNS cord-320015-lbr2q4qh 343 12 specifically specifically RB cord-320015-lbr2q4qh 343 13 suppressing suppress VBG cord-320015-lbr2q4qh 343 14 viral viral JJ cord-320015-lbr2q4qh 343 15 translation translation NN cord-320015-lbr2q4qh 343 16 and and CC cord-320015-lbr2q4qh 343 17 RNA RNA NNP cord-320015-lbr2q4qh 343 18 replication replication NN cord-320015-lbr2q4qh 343 19 Inhibition inhibition NN cord-320015-lbr2q4qh 343 20 of of IN cord-320015-lbr2q4qh 343 21 respiratory respiratory JJ cord-320015-lbr2q4qh 343 22 syncytial syncytial JJ cord-320015-lbr2q4qh 343 23 virus virus NN cord-320015-lbr2q4qh 343 24 infections infection NNS cord-320015-lbr2q4qh 343 25 with with IN cord-320015-lbr2q4qh 343 26 morpholino morpholino JJ cord-320015-lbr2q4qh 343 27 oligomers oligomer NNS cord-320015-lbr2q4qh 343 28 in in IN cord-320015-lbr2q4qh 343 29 cell cell NN cord-320015-lbr2q4qh 343 30 cultures culture NNS cord-320015-lbr2q4qh 343 31 and and CC cord-320015-lbr2q4qh 343 32 in in IN cord-320015-lbr2q4qh 343 33 mice mouse NNS cord-320015-lbr2q4qh 344 1 Antisense antisense NN cord-320015-lbr2q4qh 344 2 approaches approach VBZ cord-320015-lbr2q4qh 344 3 for for IN cord-320015-lbr2q4qh 344 4 elucidating elucidate VBG cord-320015-lbr2q4qh 344 5 ranavirus ranavirus NNP cord-320015-lbr2q4qh 344 6 gene gene NN cord-320015-lbr2q4qh 344 7 function function NN cord-320015-lbr2q4qh 344 8 in in IN cord-320015-lbr2q4qh 344 9 an an DT cord-320015-lbr2q4qh 344 10 infected infected JJ cord-320015-lbr2q4qh 344 11 fish fish NN cord-320015-lbr2q4qh 344 12 cell cell NN cord-320015-lbr2q4qh 344 13 line line NN cord-320015-lbr2q4qh 344 14 Unpublished unpublished JJ cord-320015-lbr2q4qh 344 15 work work NN cord-320015-lbr2q4qh 344 16 Inhibition inhibition NN cord-320015-lbr2q4qh 344 17 of of IN cord-320015-lbr2q4qh 344 18 red red JJ cord-320015-lbr2q4qh 344 19 seabream seabream NN cord-320015-lbr2q4qh 344 20 iridovirus iridovirus NNP cord-320015-lbr2q4qh 344 21 ( ( -LRB- cord-320015-lbr2q4qh 344 22 RSIV RSIV NNP cord-320015-lbr2q4qh 344 23 ) ) -RRB- cord-320015-lbr2q4qh 344 24 replication replication NN cord-320015-lbr2q4qh 344 25 by by IN cord-320015-lbr2q4qh 344 26 small small JJ cord-320015-lbr2q4qh 344 27 interfering interfere VBG cord-320015-lbr2q4qh 344 28 RNA RNA NNP cord-320015-lbr2q4qh 344 29 ( ( -LRB- cord-320015-lbr2q4qh 344 30 siRNA siRNA NNP cord-320015-lbr2q4qh 344 31 ) ) -RRB- cord-320015-lbr2q4qh 344 32 in in IN cord-320015-lbr2q4qh 344 33 a a DT cord-320015-lbr2q4qh 344 34 cell cell NN cord-320015-lbr2q4qh 344 35 culture culture NN cord-320015-lbr2q4qh 344 36 system system NN cord-320015-lbr2q4qh 344 37 Inhibition inhibition NN cord-320015-lbr2q4qh 344 38 of of IN cord-320015-lbr2q4qh 344 39 reporter reporter NN cord-320015-lbr2q4qh 344 40 gene gene NN cord-320015-lbr2q4qh 344 41 and and CC cord-320015-lbr2q4qh 344 42 Iridovirus Iridovirus NNP cord-320015-lbr2q4qh 344 43 - - HYPH cord-320015-lbr2q4qh 344 44 tiger tiger NNP cord-320015-lbr2q4qh 344 45 frog frog NNP cord-320015-lbr2q4qh 344 46 virus virus NN cord-320015-lbr2q4qh 344 47 in in IN cord-320015-lbr2q4qh 344 48 fish fish NN cord-320015-lbr2q4qh 344 49 cell cell NN cord-320015-lbr2q4qh 344 50 by by IN cord-320015-lbr2q4qh 344 51 RNA RNA NNP cord-320015-lbr2q4qh 344 52 interference interference NN cord-320015-lbr2q4qh 345 1 An an DT cord-320015-lbr2q4qh 345 2 ascovirus ascovirus RB cord-320015-lbr2q4qh 345 3 - - HYPH cord-320015-lbr2q4qh 345 4 encoded encode VBN cord-320015-lbr2q4qh 345 5 RNase RNase NNP cord-320015-lbr2q4qh 345 6 III III NNP cord-320015-lbr2q4qh 345 7 autoregulates autoregulate VBZ cord-320015-lbr2q4qh 345 8 its -PRON- PRP$ cord-320015-lbr2q4qh 345 9 expression expression NN cord-320015-lbr2q4qh 345 10 and and CC cord-320015-lbr2q4qh 345 11 suppresses suppress VBZ cord-320015-lbr2q4qh 345 12 RNA RNA NNP cord-320015-lbr2q4qh 345 13 interference interference NN cord-320015-lbr2q4qh 345 14 - - HYPH cord-320015-lbr2q4qh 345 15 mediated mediate VBN cord-320015-lbr2q4qh 345 16 gene gene NN cord-320015-lbr2q4qh 345 17 silencing silence VBG cord-320015-lbr2q4qh 345 18 Vaccinia Vaccinia NNP cord-320015-lbr2q4qh 345 19 virus virus NN cord-320015-lbr2q4qh 345 20 expression expression NN cord-320015-lbr2q4qh 345 21 vector vector NN cord-320015-lbr2q4qh 345 22 : : : cord-320015-lbr2q4qh 345 23 Coexpression coexpression NN cord-320015-lbr2q4qh 345 24 of of IN cord-320015-lbr2q4qh 345 25 betagalactosidase betagalactosidase NN cord-320015-lbr2q4qh 345 26 provides provide VBZ cord-320015-lbr2q4qh 345 27 visual visual JJ cord-320015-lbr2q4qh 345 28 screening screening NN cord-320015-lbr2q4qh 345 29 of of IN cord-320015-lbr2q4qh 345 30 recombinant recombinant JJ cord-320015-lbr2q4qh 345 31 virus virus NN cord-320015-lbr2q4qh 345 32 plaques plaque NNS cord-320015-lbr2q4qh 345 33 Neomycin Neomycin NNP cord-320015-lbr2q4qh 345 34 resistance resistance NN cord-320015-lbr2q4qh 345 35 as as IN cord-320015-lbr2q4qh 345 36 a a DT cord-320015-lbr2q4qh 345 37 dominant dominant JJ cord-320015-lbr2q4qh 345 38 selectable selectable JJ cord-320015-lbr2q4qh 345 39 marker marker NN cord-320015-lbr2q4qh 345 40 for for IN cord-320015-lbr2q4qh 345 41 selection selection NN cord-320015-lbr2q4qh 345 42 and and CC cord-320015-lbr2q4qh 345 43 isolation isolation NN cord-320015-lbr2q4qh 345 44 of of IN cord-320015-lbr2q4qh 345 45 vaccinia vaccinia NN cord-320015-lbr2q4qh 345 46 virus virus NN cord-320015-lbr2q4qh 345 47 recombinants recombinant NNS cord-320015-lbr2q4qh 345 48 Mapping mapping NN cord-320015-lbr2q4qh 345 49 and and CC cord-320015-lbr2q4qh 345 50 functional functional JJ cord-320015-lbr2q4qh 345 51 analysis analysis NN cord-320015-lbr2q4qh 345 52 of of IN cord-320015-lbr2q4qh 345 53 internal internal JJ cord-320015-lbr2q4qh 345 54 deletions deletion NNS cord-320015-lbr2q4qh 345 55 within within IN cord-320015-lbr2q4qh 345 56 the the DT cord-320015-lbr2q4qh 345 57 cytoplasmic cytoplasmic JJ cord-320015-lbr2q4qh 345 58 domains domain NNS cord-320015-lbr2q4qh 345 59 of of IN cord-320015-lbr2q4qh 345 60 the the DT cord-320015-lbr2q4qh 345 61 vaccinia vaccinia NN cord-320015-lbr2q4qh 345 62 virus virus NN cord-320015-lbr2q4qh 345 63 A33R a33r NN cord-320015-lbr2q4qh 345 64 and and CC cord-320015-lbr2q4qh 345 65 A36R a36r JJ cord-320015-lbr2q4qh 345 66 entry entry NN cord-320015-lbr2q4qh 345 67 proteins protein NNS cord-320015-lbr2q4qh 345 68 Promoter Promoter NNP cord-320015-lbr2q4qh 345 69 activity activity NN cord-320015-lbr2q4qh 345 70 in in IN cord-320015-lbr2q4qh 345 71 the the DT cord-320015-lbr2q4qh 345 72 5 5 CD cord-320015-lbr2q4qh 345 73 ' ' POS cord-320015-lbr2q4qh 345 74 flanking flank VBG cord-320015-lbr2q4qh 345 75 regions region NNS cord-320015-lbr2q4qh 345 76 of of IN cord-320015-lbr2q4qh 345 77 the the DT cord-320015-lbr2q4qh 345 78 Bohle Bohle NNP cord-320015-lbr2q4qh 345 79 iridovirus iridovirus NN cord-320015-lbr2q4qh 345 80 ICP ICP NNP cord-320015-lbr2q4qh 345 81 18 18 CD cord-320015-lbr2q4qh 345 82 , , , cord-320015-lbr2q4qh 345 83 ICP ICP NNP cord-320015-lbr2q4qh 345 84 46 46 CD cord-320015-lbr2q4qh 345 85 and and CC cord-320015-lbr2q4qh 345 86 major major JJ cord-320015-lbr2q4qh 345 87 capsid capsid NN cord-320015-lbr2q4qh 345 88 protein protein NN cord-320015-lbr2q4qh 345 89 genes gene NNS cord-320015-lbr2q4qh 345 90 Bohle Bohle NNP cord-320015-lbr2q4qh 345 91 iridovirus iridovirus NN cord-320015-lbr2q4qh 345 92 as as IN cord-320015-lbr2q4qh 345 93 a a DT cord-320015-lbr2q4qh 345 94 vector vector NN cord-320015-lbr2q4qh 345 95 for for IN cord-320015-lbr2q4qh 345 96 heterologous heterologous JJ cord-320015-lbr2q4qh 345 97 gene gene NN cord-320015-lbr2q4qh 345 98 expression expression NN cord-320015-lbr2q4qh 346 1 Innate innate JJ cord-320015-lbr2q4qh 346 2 immune immune JJ cord-320015-lbr2q4qh 346 3 evasion evasion NN cord-320015-lbr2q4qh 346 4 mediated mediate VBN cord-320015-lbr2q4qh 346 5 by by IN cord-320015-lbr2q4qh 346 6 the the DT cord-320015-lbr2q4qh 346 7 Ambystoma Ambystoma NNP cord-320015-lbr2q4qh 346 8 tigrinum tigrinum NNP cord-320015-lbr2q4qh 346 9 virus virus NN cord-320015-lbr2q4qh 346 10 eukaryotic eukaryotic JJ cord-320015-lbr2q4qh 346 11 translation translation NN cord-320015-lbr2q4qh 346 12 initiation initiation NN cord-320015-lbr2q4qh 346 13 factor factor NN cord-320015-lbr2q4qh 346 14 2 2 CD cord-320015-lbr2q4qh 346 15 alpha alpha NN cord-320015-lbr2q4qh 346 16 homologue homologue NN cord-320015-lbr2q4qh 347 1 The the DT cord-320015-lbr2q4qh 347 2 eIF-2 eIF-2 NNP cord-320015-lbr2q4qh 347 3 alpha alpha NN cord-320015-lbr2q4qh 347 4 protein protein NN cord-320015-lbr2q4qh 347 5 kinases kinase NNS cord-320015-lbr2q4qh 347 6 , , , cord-320015-lbr2q4qh 347 7 regulators regulator NNS cord-320015-lbr2q4qh 347 8 of of IN cord-320015-lbr2q4qh 347 9 translation translation NN cord-320015-lbr2q4qh 347 10 in in IN cord-320015-lbr2q4qh 347 11 eukaryotes eukaryote NNS cord-320015-lbr2q4qh 347 12 from from IN cord-320015-lbr2q4qh 347 13 yeast yeast NN cord-320015-lbr2q4qh 347 14 to to IN cord-320015-lbr2q4qh 347 15 humans human NNS cord-320015-lbr2q4qh 347 16 Inhibition Inhibition NNP cord-320015-lbr2q4qh 347 17 of of IN cord-320015-lbr2q4qh 347 18 PKR PKR NNP cord-320015-lbr2q4qh 347 19 by by IN cord-320015-lbr2q4qh 347 20 RNA RNA NNP cord-320015-lbr2q4qh 347 21 and and CC cord-320015-lbr2q4qh 347 22 DNA dna NN cord-320015-lbr2q4qh 347 23 viruses virus NNS cord-320015-lbr2q4qh 347 24 Improved improve VBN cord-320015-lbr2q4qh 347 25 knockout knockout NN cord-320015-lbr2q4qh 347 26 methodology methodology NN cord-320015-lbr2q4qh 347 27 reveals reveal VBZ cord-320015-lbr2q4qh 347 28 that that IN cord-320015-lbr2q4qh 347 29 Frog Frog NNP cord-320015-lbr2q4qh 347 30 virus virus NN cord-320015-lbr2q4qh 347 31 3 3 CD cord-320015-lbr2q4qh 347 32 mutants mutant NNS cord-320015-lbr2q4qh 347 33 lacking lack VBG cord-320015-lbr2q4qh 347 34 either either CC cord-320015-lbr2q4qh 347 35 the the DT cord-320015-lbr2q4qh 347 36 18 18 CD cord-320015-lbr2q4qh 347 37 K K NNP cord-320015-lbr2q4qh 347 38 immediate immediate JJ cord-320015-lbr2q4qh 347 39 - - HYPH cord-320015-lbr2q4qh 347 40 early early JJ cord-320015-lbr2q4qh 347 41 gene gene NN cord-320015-lbr2q4qh 347 42 or or CC cord-320015-lbr2q4qh 347 43 the the DT cord-320015-lbr2q4qh 347 44 truncated truncated JJ cord-320015-lbr2q4qh 347 45 vIF-2 vIF-2 NNP cord-320015-lbr2q4qh 347 46 alpha alpha NN cord-320015-lbr2q4qh 347 47 gene gene NN cord-320015-lbr2q4qh 347 48 are be VBP cord-320015-lbr2q4qh 347 49 defective defective JJ cord-320015-lbr2q4qh 347 50 for for IN cord-320015-lbr2q4qh 347 51 replication replication NN cord-320015-lbr2q4qh 347 52 in in IN cord-320015-lbr2q4qh 347 53 vivo vivo NNP cord-320015-lbr2q4qh 347 54 Construction construction NN cord-320015-lbr2q4qh 347 55 of of IN cord-320015-lbr2q4qh 347 56 green green JJ cord-320015-lbr2q4qh 347 57 fluorescent fluorescent NN cord-320015-lbr2q4qh 347 58 protein protein NN cord-320015-lbr2q4qh 347 59 - - HYPH cord-320015-lbr2q4qh 347 60 tagged tag VBN cord-320015-lbr2q4qh 347 61 recombinant recombinant JJ cord-320015-lbr2q4qh 347 62 iridovirus iridovirus NN cord-320015-lbr2q4qh 347 63 to to TO cord-320015-lbr2q4qh 347 64 assess assess VB cord-320015-lbr2q4qh 347 65 viral viral JJ cord-320015-lbr2q4qh 347 66 replication replication NN cord-320015-lbr2q4qh 347 67 Identification identification NN cord-320015-lbr2q4qh 347 68 and and CC cord-320015-lbr2q4qh 347 69 characterization characterization NN cord-320015-lbr2q4qh 347 70 of of IN cord-320015-lbr2q4qh 347 71 Singapore Singapore NNP cord-320015-lbr2q4qh 347 72 grouper grouper NN cord-320015-lbr2q4qh 347 73 iridovirus iridovirus NN cord-320015-lbr2q4qh 348 1 ( ( -LRB- cord-320015-lbr2q4qh 348 2 SGIV SGIV NNP cord-320015-lbr2q4qh 348 3 ) ) -RRB- cord-320015-lbr2q4qh 348 4 ORF162L ORF162L NNP cord-320015-lbr2q4qh 348 5 , , , cord-320015-lbr2q4qh 348 6 an an DT cord-320015-lbr2q4qh 348 7 immediate immediate JJ cord-320015-lbr2q4qh 348 8 - - HYPH cord-320015-lbr2q4qh 348 9 early early JJ cord-320015-lbr2q4qh 348 10 gene gene NN cord-320015-lbr2q4qh 348 11 involved involve VBN cord-320015-lbr2q4qh 348 12 in in IN cord-320015-lbr2q4qh 348 13 cell cell NN cord-320015-lbr2q4qh 348 14 growth growth NN cord-320015-lbr2q4qh 348 15 control control NN cord-320015-lbr2q4qh 348 16 and and CC cord-320015-lbr2q4qh 348 17 viral viral JJ cord-320015-lbr2q4qh 348 18 replication replication NN cord-320015-lbr2q4qh 348 19 ORF018R ORF018R NNP cord-320015-lbr2q4qh 348 20 , , , cord-320015-lbr2q4qh 348 21 a a DT cord-320015-lbr2q4qh 348 22 highly highly RB cord-320015-lbr2q4qh 348 23 abundant abundant JJ cord-320015-lbr2q4qh 348 24 virion virion NN cord-320015-lbr2q4qh 348 25 protein protein NN cord-320015-lbr2q4qh 348 26 from from IN cord-320015-lbr2q4qh 348 27 Singapore Singapore NNP cord-320015-lbr2q4qh 348 28 grouper grouper NN cord-320015-lbr2q4qh 348 29 iridovirus iridovirus NN cord-320015-lbr2q4qh 348 30 , , , cord-320015-lbr2q4qh 348 31 is be VBZ cord-320015-lbr2q4qh 348 32 involved involve VBN cord-320015-lbr2q4qh 348 33 in in IN cord-320015-lbr2q4qh 348 34 serine serine NN cord-320015-lbr2q4qh 348 35 / / SYM cord-320015-lbr2q4qh 348 36 threonine threonine NN cord-320015-lbr2q4qh 348 37 phosphorylation phosphorylation NN cord-320015-lbr2q4qh 348 38 and and CC cord-320015-lbr2q4qh 348 39 virion virion NN cord-320015-lbr2q4qh 348 40 assembly assembly NN cord-320015-lbr2q4qh 348 41 A a DT cord-320015-lbr2q4qh 348 42 Novel Novel NNP cord-320015-lbr2q4qh 348 43 Histone Histone NNP cord-320015-lbr2q4qh 348 44 H3 H3 NNP cord-320015-lbr2q4qh 348 45 Binding Binding NNP cord-320015-lbr2q4qh 348 46 Protein Protein NNP cord-320015-lbr2q4qh 348 47 ORF158L ORF158L NNP cord-320015-lbr2q4qh 348 48 from from IN cord-320015-lbr2q4qh 348 49 the the DT cord-320015-lbr2q4qh 348 50 Singapore Singapore NNP cord-320015-lbr2q4qh 348 51 Grouper Grouper NNP cord-320015-lbr2q4qh 348 52 Iridovirus Iridovirus NNP cord-320015-lbr2q4qh 348 53 ( ( -LRB- cord-320015-lbr2q4qh 348 54 SGIV SGIV NNP cord-320015-lbr2q4qh 348 55 ) ) -RRB- cord-320015-lbr2q4qh 348 56 Characterization characterization NN cord-320015-lbr2q4qh 348 57 of of IN cord-320015-lbr2q4qh 348 58 Singapore Singapore NNP cord-320015-lbr2q4qh 348 59 grouper grouper NN cord-320015-lbr2q4qh 348 60 iridovirus iridovirus NN cord-320015-lbr2q4qh 348 61 ( ( -LRB- cord-320015-lbr2q4qh 348 62 SGIV SGIV NNP cord-320015-lbr2q4qh 348 63 ) ) -RRB- cord-320015-lbr2q4qh 348 64 ORF086R ORF086R NNP cord-320015-lbr2q4qh 348 65 , , , cord-320015-lbr2q4qh 348 66 a a DT cord-320015-lbr2q4qh 348 67 putative putative JJ cord-320015-lbr2q4qh 348 68 homolog homolog NN cord-320015-lbr2q4qh 348 69 of of IN cord-320015-lbr2q4qh 348 70 ICP18 ICP18 NNP cord-320015-lbr2q4qh 348 71 involved involved JJ cord-320015-lbr2q4qh 348 72 in in IN cord-320015-lbr2q4qh 348 73 cell cell NN cord-320015-lbr2q4qh 348 74 growth growth NN cord-320015-lbr2q4qh 348 75 control control NN cord-320015-lbr2q4qh 348 76 and and CC cord-320015-lbr2q4qh 348 77 virus virus NN cord-320015-lbr2q4qh 348 78 replication replication NN cord-320015-lbr2q4qh 348 79 Cloning cloning NN cord-320015-lbr2q4qh 348 80 , , , cord-320015-lbr2q4qh 348 81 expression expression NN cord-320015-lbr2q4qh 348 82 and and CC cord-320015-lbr2q4qh 348 83 subcellular subcellular JJ cord-320015-lbr2q4qh 348 84 distribution distribution NN cord-320015-lbr2q4qh 348 85 of of IN cord-320015-lbr2q4qh 348 86 a a DT cord-320015-lbr2q4qh 348 87 Rana Rana NNP cord-320015-lbr2q4qh 348 88 grylio grylio NNP cord-320015-lbr2q4qh 348 89 virus virus NN cord-320015-lbr2q4qh 348 90 late late JJ cord-320015-lbr2q4qh 348 91 gene gene NN cord-320015-lbr2q4qh 348 92 encoding encode VBG cord-320015-lbr2q4qh 348 93 ERV1 erv1 JJ cord-320015-lbr2q4qh 348 94 homologue homologue NN cord-320015-lbr2q4qh 348 95 Identification identification NN cord-320015-lbr2q4qh 348 96 and and CC cord-320015-lbr2q4qh 348 97 characterization characterization NN cord-320015-lbr2q4qh 348 98 of of IN cord-320015-lbr2q4qh 348 99 a a DT cord-320015-lbr2q4qh 348 100 putative putative JJ cord-320015-lbr2q4qh 348 101 lipopolysaccharide lipopolysaccharide NN cord-320015-lbr2q4qh 348 102 - - HYPH cord-320015-lbr2q4qh 348 103 induced induce VBN cord-320015-lbr2q4qh 348 104 TNF tnf NN cord-320015-lbr2q4qh 348 105 - - HYPH cord-320015-lbr2q4qh 348 106 alpha alpha NN cord-320015-lbr2q4qh 348 107 factor factor NN cord-320015-lbr2q4qh 349 1 ( ( -LRB- cord-320015-lbr2q4qh 349 2 LITAF LITAF NNP cord-320015-lbr2q4qh 349 3 ) ) -RRB- cord-320015-lbr2q4qh 349 4 homolog homolog NN cord-320015-lbr2q4qh 349 5 from from IN cord-320015-lbr2q4qh 349 6 Singapore Singapore NNP cord-320015-lbr2q4qh 349 7 grouper grouper NN cord-320015-lbr2q4qh 349 8 iridovirus iridovirus NN cord-320015-lbr2q4qh 349 9 Constitutive constitutive JJ cord-320015-lbr2q4qh 349 10 expression expression NN cord-320015-lbr2q4qh 349 11 of of IN cord-320015-lbr2q4qh 349 12 thymidylate thymidylate NN cord-320015-lbr2q4qh 349 13 synthase synthase NN cord-320015-lbr2q4qh 349 14 from from IN cord-320015-lbr2q4qh 349 15 LCDV LCDV NNP cord-320015-lbr2q4qh 349 16 - - HYPH cord-320015-lbr2q4qh 349 17 C C NNP cord-320015-lbr2q4qh 349 18 induces induce VBZ cord-320015-lbr2q4qh 349 19 a a DT cord-320015-lbr2q4qh 349 20 transformed transform VBN cord-320015-lbr2q4qh 349 21 phenoptype phenoptype NN cord-320015-lbr2q4qh 349 22 in in IN cord-320015-lbr2q4qh 349 23 fish fish NN cord-320015-lbr2q4qh 349 24 cells cell NNS cord-320015-lbr2q4qh 350 1 We -PRON- PRP cord-320015-lbr2q4qh 350 2 would would MD cord-320015-lbr2q4qh 350 3 like like VB cord-320015-lbr2q4qh 350 4 to to TO cord-320015-lbr2q4qh 350 5 thank thank VB cord-320015-lbr2q4qh 350 6 Robert Robert NNP cord-320015-lbr2q4qh 350 7 Sample Sample NNP cord-320015-lbr2q4qh 350 8 for for IN cord-320015-lbr2q4qh 350 9 the the DT cord-320015-lbr2q4qh 350 10 electron electron NN cord-320015-lbr2q4qh 350 11 micrographs micrograph NNS cord-320015-lbr2q4qh 350 12 shown show VBN cord-320015-lbr2q4qh 350 13 in in IN cord-320015-lbr2q4qh 350 14 Figure Figure NNP cord-320015-lbr2q4qh 350 15 1 1 CD cord-320015-lbr2q4qh 350 16 and and CC cord-320015-lbr2q4qh 350 17 the the DT cord-320015-lbr2q4qh 350 18 artwork artwork NN cord-320015-lbr2q4qh 350 19 depicted depict VBN cord-320015-lbr2q4qh 350 20 in in IN cord-320015-lbr2q4qh 350 21 Figure figure NN cord-320015-lbr2q4qh 350 22 2 2 CD cord-320015-lbr2q4qh 350 23 . . . cord-320015-lbr2q4qh 351 1 The the DT cord-320015-lbr2q4qh 351 2 work work NN cord-320015-lbr2q4qh 351 3 was be VBD cord-320015-lbr2q4qh 351 4 partially partially RB cord-320015-lbr2q4qh 351 5 supported support VBN cord-320015-lbr2q4qh 351 6 by by IN cord-320015-lbr2q4qh 351 7 NSF NSF NNP cord-320015-lbr2q4qh 351 8 award award NN cord-320015-lbr2q4qh 352 1 No no UH cord-320015-lbr2q4qh 352 2 . . . cord-320015-lbr2q4qh 353 1 IOS IOS NNP cord-320015-lbr2q4qh 353 2 07 07 CD cord-320015-lbr2q4qh 353 3 - - HYPH cord-320015-lbr2q4qh 353 4 42711 42711 CD cord-320015-lbr2q4qh 353 5 . . . cord-320015-lbr2q4qh 354 1 The the DT cord-320015-lbr2q4qh 354 2 authors author NNS cord-320015-lbr2q4qh 354 3 declare declare VBP cord-320015-lbr2q4qh 354 4 no no DT cord-320015-lbr2q4qh 354 5 conflict conflict NN cord-320015-lbr2q4qh 354 6 of of IN cord-320015-lbr2q4qh 354 7 interest interest NN cord-320015-lbr2q4qh 354 8 . . .