id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004518-jd1wxobz	Běláková, Jana	DNA vaccines: are they still just a powerful tool for the future?	2007-12-03		.txt	text/plain	8107	409	40	Immunization with plasmid DNA (DNA-based vaccination) is a relatively novel technique for the efficacious stimulation of a specific cellular and humoral immune response to protein antigens. The application of DNA vaccine to the liver is associated with enormous protein expression followed by a strong antibody-and cell-mediated immune response. One highly efficacious delivery system for DNA vaccines, or, more precisely, genetic vaccines, is based on recombinant viral vectors derived either from attenuated viruses used for preventive vaccination (vaccinia, poliovirus, hepatitis B virus, measles virus) or from viruses such as human adenovirus (HAdV), adeno-associated virus (AAV), alphavirus, vesicular stomatitis virus (VSV), or poxviruses other than vaccinia [41] . In animal experiments a recombinant measles vector expressing HIV-1 envelope antigen induced neutralizing antibodies and envelope-specific CD4 + and CD8 + cell responses after a single dose [67] . Although some mucosal response is detectable after a systemic DNA vaccination, i.m. injection and gene-gun delivery of plasmid DNA have a limited ability to induce mucosal immune responses [99] .	./cache/cord-004518-jd1wxobz.txt	./txt/cord-004518-jd1wxobz.txt