id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001260-6krujv2m	Bremer, Paul T.	Injection Route and TLR9 Agonist Addition Significantly Impact Heroin Vaccine Efficacy	2014-02-11		.txt	text/plain	3612	206	50	To thoroughly assess vaccine efficacy, full dose–response curves were generated for heroin-induced analgesia in both hot plate and tail immersion tests. In the research we disclose herein, both injection route and TLR9 agonist CpG ODN 1826 significantly affected antibody titer levels and opiate affinity, translating to marked differences in mitigation of heroin-induced analgesia. Mice were immunized with our first generation heroin vaccine (heroin− KLH conjugate formulated with alum adjuvant), and antibody titers were measured by ELISA against heroin−BSA conjugate. The current study highlights the impact of varying the injection route of our first generation heroin vaccine, along with enhancing immunogenicity by addition of the CpG ODN adjuvant. We have shown that the enhanced antidrug titers and opioid affinities from CpG ODN translate directly to reduced pharmacodynamics of the drug; in our case heroin-induced latency to nociception (analgesia) was greatly diminished in hot plate and tail immersion tests.	./cache/cord-001260-6krujv2m.txt	./txt/cord-001260-6krujv2m.txt