id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-345430-yowyy5nr	Balzano, Francesca	Behavioral Changes in Stem-Cell Potency by HepG2-Exhausted Medium	2020-08-12		.txt	text/plain	5469	310	52	Moreover, we observed a downregulation of DNMT1, and a simultaneous overexpression of Oct-4 and c-Myc. These findings suggest that WJ-MSCs are more likely to retain a stem phenotype and sometimes to switch to a highly undifferentiable proliferative-like behavior if treated with medium exhausted by human HepG2 cell lines. Here, we investigated the relative expression levels of the c-Myc, Oct-4, DNMT1, NANOG, SIRT1, p53, SOX2, GAPDH, p21waf1, p19, p16, and TERT genes in WJ-MSCs before and after treatment with HepG2-exhausted medium. Our results showed a path in which WJ-MSCs exposed to medium exhausted by HepG2 cells exhibited an increase in the expression levels of c-Myc, SIRT1, and GAPDH, and a simultaneous decrease in p53 and p21 gene expression ( Figure 2 ). Here, we highlight that p21 downregulation in treated WJ-MSCs (as compared to untreated controls) is related to an increase in cell proliferation, further implied by the greater expression of the stemness gene c-Myc, able itself to inhibit p21 [62, 63] .	./cache/cord-345430-yowyy5nr.txt	./txt/cord-345430-yowyy5nr.txt