id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293747-ds8rhbkv	Lani, Rafidah	Antiviral activity of silymarin against chikungunya virus	2015-06-16		.txt	text/plain	5074	254	51	Three compounds: silymarin, quercetin and kaempferol were evaluated for their in vitro antiviral activities against CHIKV using a CHIKV replicon cell line and clinical isolate of CHIKV of Central/East African genotype. A cytopathic effect inhibition assay was used to determine their activities on CHIKV viral replication and quantitative reverse transcription PCR was used to calculate virus yield. Different non-cytotoxic concentrations of silymarin, kaempferol and quercetin were tested on CHIKV-infected Vero cells to find the effective compound. To confirm the post-entry antiviral activity of silymarin against CHIKV a virus yield assay using qRT-PCR was used. In contrast, dose-dependent reduction of amounts of nsP1, nsP3 and E2 proteins was observed (Fig. 6) indicating that silymarin limited CHIKV replication and virus-encoded protein synthesis within the treated cells. However, as in virus expression and replicon cell lines the synthesis of viral RNAs and proteins are coupled further study is necessary to evaluate the direct effect of silymarin on inhibition of newly synthesized CHIKV proteins.	./cache/cord-293747-ds8rhbkv.txt	./txt/cord-293747-ds8rhbkv.txt