id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292231-vxaqizkj	Bouquet, Jerome	Microbial burden and viral exacerbations in a longitudinal multicenter COPD cohort	2020-03-30		.txt	text/plain	5746	318	34	We performed nucleic acid detection for 20 respiratory viruses and 16S ribosomal RNA gene sequencing to evaluate the bacterial microbiota in 1179 sputum samples collected at stable, acute exacerbation and follow-up visits. The odds ratio of an acute exacerbation event and frequent exacerbations (≥2 events/ year) was calculated for demographic and clinical data, viral infections and abundance of certain bacterial taxa in the lung microbiota (Fig. 3 ). Microbiota variability in seemingly stable disease state were associated with higher exacerbation frequency and frequent viral infections (Fig. 4b) . Samples with high relative abundance of Streptococcus/Rothia (biotype 2) were found in greater proportion in USA patients, which was associated with longer history of COPD and less frequent detection of viruses at stable visits (Fig. 6a) . Streptococcus and Haemophilus were found in a majority of USA samples, and associated with longer COPD duration, higher exacerbation frequency, antibiotics and corticosteroid use, but did not significantly correlate with higher viral infections (Fig. 6b) .	./cache/cord-292231-vxaqizkj.txt	./txt/cord-292231-vxaqizkj.txt