id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-013325-8lds7nti	Ecker, Jeffrey W.	High-Yield Expression and Purification of Recombinant Influenza Virus Proteins from Stably-Transfected Mammalian Cell Lines	2020-08-21		.txt	text/plain	9393	426	43	title: High-Yield Expression and Purification of Recombinant Influenza Virus Proteins from Stably-Transfected Mammalian Cell Lines Herein, we describe the approach for developing stable transfected human cell lines for the expression of recombinant influenza virus hemagglutinin (HA) and recombinant influenza virus neuraminidase (NA) proteins for the purpose of in vitro and in vivo vaccine development. Herein, we describe an approach for developing stable transfected human cell lines expressing recombinant influenza virus hemagglutinin (HA) and influenza virus neuraminidase (NA) proteins for the purpose of in vitro and in vivo vaccine development. Plasmids for expressing recombinant wild-type HA influenza proteins were human codon optimized and ordered from Genewiz (South Plainfield, NJ, USA) or cloned from a full-length HA gene into a pcDNA3.1/Zeo (+) vector (Thermo Fisher Scientific) using the BamHI and HindIII restriction sites. As shown in Figure 4B , the maximal rHA yields were achieved by the previously described Computationally-Optimized Broadly Reactive Antigen (COBRA) P1 HA protein [3, 24] stable transfected cells.	./cache/cord-013325-8lds7nti.txt	./txt/cord-013325-8lds7nti.txt