id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-009439-wvlczk62	Park, Hyun-Jun	The essential role of fructose-1,6-bisphosphatase 2 enzyme in thermal homeostasis upon cold stress	2020-03-16		.txt	text/plain	6171	341	48	To measure in vivo whole-body and tissuespecific glucose fluxes under feeding-mimicking conditions where insulin and exogenous glucose are supplied, we performed hyperinsulinemic-euglycemic clamp studies on WT and Fbp2 KO mice as previously reported 23, 30 . A decreased muscle glycogen content (Fig. 3d) and energy metabolism (Fig. 1) in KO mice were observed only after fasting; therefore, we assumed that Fbp2 might have more crucial roles in the severe glycogenolytic condition. Thus, both at room temperature and after 6 h of cold challenge, we conducted experiments using a radioisotope-labeled tracer ( 14 C(U)-lactate) to quantitatively measure the incorporation of lactate into glycogen in the skeletal muscle of WT and Fbp2 KO mice (Fig. 4a) . Although we focused on the relationship between cold tolerance and intramuscular glyconeogenesis, the physiological role of Fbp2 may be extended to other conditions where skeletal muscle glycogen breakdown replenishment is increased.	./cache/cord-009439-wvlczk62.txt	./txt/cord-009439-wvlczk62.txt