id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-006771-n1w56x2m	Liu, Chien-Ying	Apoptotic neutrophils undergoing secondary necrosis induce human lung epithelial cell detachment	2003-10-17		.txt	text/plain	4661	264	33	title: Apoptotic neutrophils undergoing secondary necrosis induce human lung epithelial cell detachment However, the apoptosing neutrophil-induced detachment of airway epithelial cells was completely abrogated by exogenous SLPI, a specific inhibitor of serine proteinases ( fig. To determine whether early apoptotic neutrophils release functional elastase, we examined elastase activity in supernatant from the annexin V-selected fraction cultured in vitro, and the data revealed that elastase activity continually increased with time ( fig. Our study demonstrates that early apoptotic neutrophils induce lung epithelial cell detachment. Furthermore, the timing of this release correlates with the progression of early apoptotic neutrophils to secondary necrosis and lung epithelial cell detachment in vitro. On the other hand, SLPI, one of the endogenous serine proteinase-specific antiproteinase defenses, abrogates both live, activated neutrophil-and apoptosing neutrophil-induced lung epithelial cell detachment. In conclusion, this study demonstrates that apoptosing neutrophils release, at least, functional elastase activity during secondary necrosis and induce a cytotoxic effect on airway epithelial cells.	./cache/cord-006771-n1w56x2m.txt	./txt/cord-006771-n1w56x2m.txt