id author title date pages extension mime words sentences flesch summary cache txt cord-000698-v9k7vpi8 St. Leger, Judy West Nile Virus Infection in Killer Whale, Texas, USA, 2007 2011-08-17 .txt text/plain 1403 100 46 title: West Nile Virus Infection in Killer Whale, Texas, USA, 2007 In 2007, nonsuppurative encephalitis was identified in a killer whale at a Texas, USA, marine park. Panviral DNA microarray of brain tissue suggested West Nile virus (WNV); WNV was confirmed by reverse transcription PCR and sequencing. Among marine mammals, WNV infection has been reported in a harbor seal (Phoca vitulina) (1) . Meninges demonstrated moderate focally extensive and multifocal areas of acute meningeal congestion and hemorrhage. This staining demonstrated abundant WNV antigen within the cytoplasm of a small number of neurons and glial cells and in fewer macrophages in the brain tissue (Figure) . We demonstrate that WNV can infect and cause disease in killer whales. The serologic results demonstrate that subclinical infections can occur and that exposure can be variable. Serologic screening of bottlenose dolphins (Tursiops truncatus) from the Indian River Lagoon demonstrated WNV titers (7) . ./cache/cord-000698-v9k7vpi8.txt ./txt/cord-000698-v9k7vpi8.txt