id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-266488-wc6k06sm	Feng, Mingqian	Construction and next-generation sequencing analysis of a large phage-displayed V(NAR) single-domain antibody library from six naïve nurse sharks	2018-11-07		.txt	text/plain	6552	351	58	title: Construction and next-generation sequencing analysis of a large phage-displayed V(NAR) single-domain antibody library from six naïve nurse sharks METHODS: Here, we developed a library construction method based on polymerase chain reaction (PCR)-Extension Assembly and Self-Ligation (named "EASeL") to construct a large V(NAR) antibody library with a size of 1.2 × 10(10) from six naïve adult nurse sharks (Ginglymostoma cirratum). To validate the use of the shark V(NAR) library for antibody discovery, we isolated a panel of V(NAR) phage binders to cancer therapy-related antigens, including glypican-3, human epidermal growth factor receptor 2 (HER2), and programmed cell death-1 (PD1). The isolated shark single-domain antibodies including Type I and Type II V(NAR)s were produced in Escherichia coli and validated for their antigen binding. In this study, we developed a PCR-Extension Assembly and Self-Ligation-based method (named EASeL) to make a large phage-displayed V NAR single-domain library from six nurse sharks.	./cache/cord-266488-wc6k06sm.txt	./txt/cord-266488-wc6k06sm.txt