id sid tid token lemma pos cord-310467-v2wlu5eq 1 1 key key JJ cord-310467-v2wlu5eq 2 1 : : : cord-310467-v2wlu5eq 2 2 cord-310467-v2wlu5eq cord-310467-v2wlu5eq NN cord-310467-v2wlu5eq 2 3 authors author NNS cord-310467-v2wlu5eq 2 4 : : : cord-310467-v2wlu5eq 2 5 Tô Tô NNP cord-310467-v2wlu5eq 2 6 , , , cord-310467-v2wlu5eq 2 7 Long Long NNP cord-310467-v2wlu5eq 2 8 - - HYPH cord-310467-v2wlu5eq 2 9 Thành Thành NNP cord-310467-v2wlu5eq 2 10 ; ; : cord-310467-v2wlu5eq 2 11 Bernard Bernard NNP cord-310467-v2wlu5eq 2 12 , , , cord-310467-v2wlu5eq 2 13 Serge Serge NNP cord-310467-v2wlu5eq 2 14 title title NN cord-310467-v2wlu5eq 2 15 : : : cord-310467-v2wlu5eq 3 1 Effect effect NN cord-310467-v2wlu5eq 3 2 of of IN cord-310467-v2wlu5eq 3 3 fixation fixation NN cord-310467-v2wlu5eq 3 4 on on IN cord-310467-v2wlu5eq 3 5 the the DT cord-310467-v2wlu5eq 3 6 detection detection NN cord-310467-v2wlu5eq 3 7 of of IN cord-310467-v2wlu5eq 3 8 transmissible transmissible JJ cord-310467-v2wlu5eq 3 9 gastroenteritis gastroenteritis JJ cord-310467-v2wlu5eq 3 10 coronavirus coronavirus NN cord-310467-v2wlu5eq 3 11 antigens antigen NNS cord-310467-v2wlu5eq 3 12 by by IN cord-310467-v2wlu5eq 3 13 the the DT cord-310467-v2wlu5eq 3 14 fixed fix VBN cord-310467-v2wlu5eq 3 15 - - HYPH cord-310467-v2wlu5eq 3 16 cell cell NN cord-310467-v2wlu5eq 3 17 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 3 18 technique technique NN cord-310467-v2wlu5eq 3 19 date date NN cord-310467-v2wlu5eq 3 20 : : : cord-310467-v2wlu5eq 3 21 1992 1992 CD cord-310467-v2wlu5eq 3 22 - - SYM cord-310467-v2wlu5eq 3 23 10 10 CD cord-310467-v2wlu5eq 3 24 - - SYM cord-310467-v2wlu5eq 3 25 02 02 CD cord-310467-v2wlu5eq 3 26 journal journal NN cord-310467-v2wlu5eq 3 27 : : : cord-310467-v2wlu5eq 3 28 Journal Journal NNP cord-310467-v2wlu5eq 3 29 of of IN cord-310467-v2wlu5eq 3 30 Immunological Immunological NNP cord-310467-v2wlu5eq 3 31 Methods Methods NNPS cord-310467-v2wlu5eq 3 32 DOI DOI NNP cord-310467-v2wlu5eq 3 33 : : : cord-310467-v2wlu5eq 3 34 10.1016/0022 10.1016/0022 CD cord-310467-v2wlu5eq 3 35 - - HYPH cord-310467-v2wlu5eq 3 36 1759(92)90192-v 1759(92)90192-v CD cord-310467-v2wlu5eq 3 37 sha sha NNP cord-310467-v2wlu5eq 3 38 : : : cord-310467-v2wlu5eq 3 39 d1db70b1ddae9d7ad593910d9619cb6d6e46de0d d1db70b1ddae9d7ad593910d9619cb6d6e46de0d NNP cord-310467-v2wlu5eq 3 40 doc_id doc_id NNP cord-310467-v2wlu5eq 3 41 : : : cord-310467-v2wlu5eq 3 42 310467 310467 CD cord-310467-v2wlu5eq 3 43 cord_uid cord_uid NN cord-310467-v2wlu5eq 3 44 : : : cord-310467-v2wlu5eq 4 1 v2wlu5eq v2wlu5eq NNP cord-310467-v2wlu5eq 4 2 Abstract Abstract NNP cord-310467-v2wlu5eq 5 1 The the DT cord-310467-v2wlu5eq 5 2 effect effect NN cord-310467-v2wlu5eq 5 3 of of IN cord-310467-v2wlu5eq 5 4 various various JJ cord-310467-v2wlu5eq 5 5 fixatives fixative NNS cord-310467-v2wlu5eq 5 6 and and CC cord-310467-v2wlu5eq 5 7 detergents detergent NNS cord-310467-v2wlu5eq 5 8 on on IN cord-310467-v2wlu5eq 5 9 the the DT cord-310467-v2wlu5eq 5 10 in in FW cord-310467-v2wlu5eq 5 11 vitro vitro FW cord-310467-v2wlu5eq 5 12 detection detection NN cord-310467-v2wlu5eq 5 13 of of IN cord-310467-v2wlu5eq 5 14 the the DT cord-310467-v2wlu5eq 5 15 viral viral JJ cord-310467-v2wlu5eq 5 16 determinants determinant NNS cord-310467-v2wlu5eq 5 17 which which WDT cord-310467-v2wlu5eq 5 18 are be VBP cord-310467-v2wlu5eq 5 19 expressed express VBN cord-310467-v2wlu5eq 5 20 in in IN cord-310467-v2wlu5eq 5 21 swine swine NN cord-310467-v2wlu5eq 5 22 testis testis NN cord-310467-v2wlu5eq 5 23 cells cell NNS cord-310467-v2wlu5eq 5 24 infected infect VBN cord-310467-v2wlu5eq 5 25 with with IN cord-310467-v2wlu5eq 5 26 the the DT cord-310467-v2wlu5eq 5 27 transmissible transmissible JJ cord-310467-v2wlu5eq 5 28 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 5 29 coronavirus coronavirus NN cord-310467-v2wlu5eq 5 30 ( ( -LRB- cord-310467-v2wlu5eq 5 31 TGEV TGEV NNP cord-310467-v2wlu5eq 5 32 ) ) -RRB- cord-310467-v2wlu5eq 5 33 was be VBD cord-310467-v2wlu5eq 5 34 studied study VBN cord-310467-v2wlu5eq 5 35 using use VBG cord-310467-v2wlu5eq 5 36 a a DT cord-310467-v2wlu5eq 5 37 microwell microwell NNP cord-310467-v2wlu5eq 5 38 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 5 39 technique technique NN cord-310467-v2wlu5eq 5 40 . . . cord-310467-v2wlu5eq 6 1 When when WRB cord-310467-v2wlu5eq 6 2 compared compare VBN cord-310467-v2wlu5eq 6 3 with with IN cord-310467-v2wlu5eq 6 4 glutaraldehyde glutaraldehyde NN cord-310467-v2wlu5eq 6 5 and and CC cord-310467-v2wlu5eq 6 6 formaldehyde formaldehyde NN cord-310467-v2wlu5eq 6 7 , , , cord-310467-v2wlu5eq 6 8 0.1 0.1 CD cord-310467-v2wlu5eq 6 9 % % NN cord-310467-v2wlu5eq 6 10 paraformaldehyde paraformaldehyde NNP cord-310467-v2wlu5eq 6 11 was be VBD cord-310467-v2wlu5eq 6 12 found find VBN cord-310467-v2wlu5eq 6 13 to to TO cord-310467-v2wlu5eq 6 14 be be VB cord-310467-v2wlu5eq 6 15 the the DT cord-310467-v2wlu5eq 6 16 fixative fixative NN cord-310467-v2wlu5eq 6 17 of of IN cord-310467-v2wlu5eq 6 18 choice choice NN cord-310467-v2wlu5eq 6 19 for for IN cord-310467-v2wlu5eq 6 20 the the DT cord-310467-v2wlu5eq 6 21 detection detection NN cord-310467-v2wlu5eq 6 22 of of IN cord-310467-v2wlu5eq 6 23 these these DT cord-310467-v2wlu5eq 6 24 determinants determinant NNS cord-310467-v2wlu5eq 6 25 on on IN cord-310467-v2wlu5eq 6 26 the the DT cord-310467-v2wlu5eq 6 27 membranes membrane NNS cord-310467-v2wlu5eq 6 28 of of IN cord-310467-v2wlu5eq 6 29 infected infected JJ cord-310467-v2wlu5eq 6 30 cells cell NNS cord-310467-v2wlu5eq 6 31 . . . cord-310467-v2wlu5eq 7 1 Among among IN cord-310467-v2wlu5eq 7 2 dehydrating dehydrate VBG cord-310467-v2wlu5eq 7 3 fixatives fixative NNS cord-310467-v2wlu5eq 7 4 , , , cord-310467-v2wlu5eq 7 5 80 80 CD cord-310467-v2wlu5eq 7 6 % % NN cord-310467-v2wlu5eq 7 7 acetone acetone NN cord-310467-v2wlu5eq 7 8 or or CC cord-310467-v2wlu5eq 7 9 a a DT cord-310467-v2wlu5eq 7 10 mixture mixture NN cord-310467-v2wlu5eq 7 11 of of IN cord-310467-v2wlu5eq 7 12 acetone acetone NN cord-310467-v2wlu5eq 7 13 and and CC cord-310467-v2wlu5eq 7 14 ethanol ethanol NN cord-310467-v2wlu5eq 7 15 or or CC cord-310467-v2wlu5eq 7 16 of of IN cord-310467-v2wlu5eq 7 17 acetone acetone NN cord-310467-v2wlu5eq 7 18 , , , cord-310467-v2wlu5eq 7 19 methanol methanol NN cord-310467-v2wlu5eq 7 20 and and CC cord-310467-v2wlu5eq 7 21 ethanol ethanol NN cord-310467-v2wlu5eq 7 22 were be VBD cord-310467-v2wlu5eq 7 23 found find VBN cord-310467-v2wlu5eq 7 24 to to TO cord-310467-v2wlu5eq 7 25 be be VB cord-310467-v2wlu5eq 7 26 the the DT cord-310467-v2wlu5eq 7 27 best good JJS cord-310467-v2wlu5eq 7 28 fixatives fixative NNS cord-310467-v2wlu5eq 7 29 for for IN cord-310467-v2wlu5eq 7 30 the the DT cord-310467-v2wlu5eq 7 31 detection detection NN cord-310467-v2wlu5eq 7 32 of of IN cord-310467-v2wlu5eq 7 33 these these DT cord-310467-v2wlu5eq 7 34 viral viral JJ cord-310467-v2wlu5eq 7 35 determinants determinant NNS cord-310467-v2wlu5eq 7 36 which which WDT cord-310467-v2wlu5eq 7 37 are be VBP cord-310467-v2wlu5eq 7 38 expressed express VBN cord-310467-v2wlu5eq 7 39 in in IN cord-310467-v2wlu5eq 7 40 infected infected JJ cord-310467-v2wlu5eq 7 41 cells cell NNS cord-310467-v2wlu5eq 7 42 . . . cord-310467-v2wlu5eq 8 1 In in IN cord-310467-v2wlu5eq 8 2 the the DT cord-310467-v2wlu5eq 8 3 case case NN cord-310467-v2wlu5eq 8 4 of of IN cord-310467-v2wlu5eq 8 5 acetone acetone NN cord-310467-v2wlu5eq 8 6 , , , cord-310467-v2wlu5eq 8 7 the the DT cord-310467-v2wlu5eq 8 8 temperature temperature NN cord-310467-v2wlu5eq 8 9 of of IN cord-310467-v2wlu5eq 8 10 fixation fixation NN cord-310467-v2wlu5eq 8 11 and and CC cord-310467-v2wlu5eq 8 12 its -PRON- PRP$ cord-310467-v2wlu5eq 8 13 concentration concentration NN cord-310467-v2wlu5eq 8 14 in in IN cord-310467-v2wlu5eq 8 15 the the DT cord-310467-v2wlu5eq 8 16 fixative fixative NN cord-310467-v2wlu5eq 8 17 preparation preparation NN cord-310467-v2wlu5eq 8 18 were be VBD cord-310467-v2wlu5eq 8 19 found find VBN cord-310467-v2wlu5eq 8 20 to to TO cord-310467-v2wlu5eq 8 21 be be VB cord-310467-v2wlu5eq 8 22 important important JJ cord-310467-v2wlu5eq 8 23 . . . cord-310467-v2wlu5eq 9 1 The the DT cord-310467-v2wlu5eq 9 2 treatment treatment NN cord-310467-v2wlu5eq 9 3 of of IN cord-310467-v2wlu5eq 9 4 0.05 0.05 CD cord-310467-v2wlu5eq 9 5 % % NN cord-310467-v2wlu5eq 9 6 glutaraldehyde glutaraldehyde NN cord-310467-v2wlu5eq 9 7 - - HYPH cord-310467-v2wlu5eq 9 8 fixed fix VBN cord-310467-v2wlu5eq 9 9 , , , cord-310467-v2wlu5eq 9 10 infected infected JJ cord-310467-v2wlu5eq 9 11 cells cell NNS cord-310467-v2wlu5eq 9 12 with with IN cord-310467-v2wlu5eq 9 13 0.1 0.1 CD cord-310467-v2wlu5eq 9 14 % % NN cord-310467-v2wlu5eq 9 15 saponin saponin NN cord-310467-v2wlu5eq 9 16 or or CC cord-310467-v2wlu5eq 9 17 0.1 0.1 CD cord-310467-v2wlu5eq 9 18 % % NN cord-310467-v2wlu5eq 9 19 paraformaldehyde paraformaldehyde NN cord-310467-v2wlu5eq 9 20 - - HYPH cord-310467-v2wlu5eq 9 21 fixed fix VBN cord-310467-v2wlu5eq 9 22 , , , cord-310467-v2wlu5eq 9 23 infected infected JJ cord-310467-v2wlu5eq 9 24 cells cell NNS cord-310467-v2wlu5eq 9 25 with with IN cord-310467-v2wlu5eq 9 26 1%NP-40 1%np-40 CD cord-310467-v2wlu5eq 9 27 led lead VBD cord-310467-v2wlu5eq 9 28 to to IN cord-310467-v2wlu5eq 9 29 satisfactory satisfactory JJ cord-310467-v2wlu5eq 9 30 detection detection NN cord-310467-v2wlu5eq 9 31 of of IN cord-310467-v2wlu5eq 9 32 viral viral JJ cord-310467-v2wlu5eq 9 33 determinants determinant NNS cord-310467-v2wlu5eq 9 34 . . . cord-310467-v2wlu5eq 10 1 Using use VBG cord-310467-v2wlu5eq 10 2 Triton Triton NNP cord-310467-v2wlu5eq 10 3 X-100 X-100 NNS cord-310467-v2wlu5eq 10 4 to to TO cord-310467-v2wlu5eq 10 5 render render VB cord-310467-v2wlu5eq 10 6 cells cell NNS cord-310467-v2wlu5eq 10 7 permeable permeable JJ cord-310467-v2wlu5eq 10 8 , , , cord-310467-v2wlu5eq 10 9 the the DT cord-310467-v2wlu5eq 10 10 quantities quantity NNS cord-310467-v2wlu5eq 10 11 of of IN cord-310467-v2wlu5eq 10 12 N N NNP cord-310467-v2wlu5eq 10 13 and and CC cord-310467-v2wlu5eq 10 14 M M NNP cord-310467-v2wlu5eq 10 15 antigen antigen NN cord-310467-v2wlu5eq 10 16 detected detect VBD cord-310467-v2wlu5eq 10 17 in in IN cord-310467-v2wlu5eq 10 18 TGEV TGEV NNP cord-310467-v2wlu5eq 10 19 - - HYPH cord-310467-v2wlu5eq 10 20 infected infect VBN cord-310467-v2wlu5eq 10 21 cells cell NNS cord-310467-v2wlu5eq 10 22 prefixed prefix VBN cord-310467-v2wlu5eq 10 23 with with IN cord-310467-v2wlu5eq 10 24 either either DT cord-310467-v2wlu5eq 10 25 0.05 0.05 CD cord-310467-v2wlu5eq 10 26 % % NN cord-310467-v2wlu5eq 10 27 glutaraldehyde glutaraldehyde NN cord-310467-v2wlu5eq 10 28 or or CC cord-310467-v2wlu5eq 10 29 0.1 0.1 CD cord-310467-v2wlu5eq 10 30 % % NN cord-310467-v2wlu5eq 10 31 paraformaldehyde paraformaldehyde NNP cord-310467-v2wlu5eq 10 32 were be VBD cord-310467-v2wlu5eq 10 33 equal equal JJ cord-310467-v2wlu5eq 10 34 to to IN cord-310467-v2wlu5eq 10 35 those those DT cord-310467-v2wlu5eq 10 36 of of IN cord-310467-v2wlu5eq 10 37 80 80 CD cord-310467-v2wlu5eq 10 38 % % NN cord-310467-v2wlu5eq 10 39 acetone acetone NN cord-310467-v2wlu5eq 10 40 - - HYPH cord-310467-v2wlu5eq 10 41 fixed fix VBN cord-310467-v2wlu5eq 10 42 , , , cord-310467-v2wlu5eq 10 43 TGEV TGEV NNP cord-310467-v2wlu5eq 10 44 - - HYPH cord-310467-v2wlu5eq 10 45 infected infect VBN cord-310467-v2wlu5eq 10 46 cells cell NNS cord-310467-v2wlu5eq 10 47 while while IN cord-310467-v2wlu5eq 10 48 the the DT cord-310467-v2wlu5eq 10 49 quantity quantity NN cord-310467-v2wlu5eq 10 50 of of IN cord-310467-v2wlu5eq 10 51 S S NNP cord-310467-v2wlu5eq 10 52 antigen antigen NN cord-310467-v2wlu5eq 10 53 detected detect VBN cord-310467-v2wlu5eq 10 54 was be VBD cord-310467-v2wlu5eq 10 55 diminished diminish VBN cord-310467-v2wlu5eq 10 56 . . . cord-310467-v2wlu5eq 11 1 The the DT cord-310467-v2wlu5eq 11 2 effect effect NN cord-310467-v2wlu5eq 11 3 of of IN cord-310467-v2wlu5eq 11 4 other other JJ cord-310467-v2wlu5eq 11 5 detergents detergent NNS cord-310467-v2wlu5eq 11 6 such such JJ cord-310467-v2wlu5eq 11 7 as as IN cord-310467-v2wlu5eq 11 8 zwittergent zwittergent NNP cord-310467-v2wlu5eq 11 9 , , , cord-310467-v2wlu5eq 11 10 embigen embigen NNP cord-310467-v2wlu5eq 11 11 BB BB NNP cord-310467-v2wlu5eq 11 12 , , , cord-310467-v2wlu5eq 11 13 Chaps Chaps NNPS cord-310467-v2wlu5eq 11 14 and and CC cord-310467-v2wlu5eq 11 15 N N NNP cord-310467-v2wlu5eq 11 16 - - HYPH cord-310467-v2wlu5eq 11 17 lauroylsarcosine lauroylsarcosine NN cord-310467-v2wlu5eq 11 18 on on IN cord-310467-v2wlu5eq 11 19 the the DT cord-310467-v2wlu5eq 11 20 detection detection NN cord-310467-v2wlu5eq 11 21 of of IN cord-310467-v2wlu5eq 11 22 viral viral JJ cord-310467-v2wlu5eq 11 23 determinants determinant NNS cord-310467-v2wlu5eq 11 24 was be VBD cord-310467-v2wlu5eq 11 25 also also RB cord-310467-v2wlu5eq 11 26 studied study VBN cord-310467-v2wlu5eq 11 27 . . . cord-310467-v2wlu5eq 12 1 Transmissible transmissible JJ cord-310467-v2wlu5eq 12 2 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 12 3 ( ( -LRB- cord-310467-v2wlu5eq 12 4 TGE TGE NNP cord-310467-v2wlu5eq 12 5 ) ) -RRB- cord-310467-v2wlu5eq 12 6 is be VBZ cord-310467-v2wlu5eq 12 7 a a DT cord-310467-v2wlu5eq 12 8 highly highly RB cord-310467-v2wlu5eq 12 9 contagious contagious JJ cord-310467-v2wlu5eq 12 10 enteric enteric JJ cord-310467-v2wlu5eq 12 11 infection infection NN cord-310467-v2wlu5eq 12 12 of of IN cord-310467-v2wlu5eq 12 13 swine swine NN cord-310467-v2wlu5eq 12 14 caused cause VBN cord-310467-v2wlu5eq 12 15 by by IN cord-310467-v2wlu5eq 12 16 transmissible transmissible JJ cord-310467-v2wlu5eq 12 17 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 12 18 coronavirus coronavirus NN cord-310467-v2wlu5eq 12 19 ( ( -LRB- cord-310467-v2wlu5eq 12 20 TGEV TGEV NNP cord-310467-v2wlu5eq 12 21 ) ) -RRB- cord-310467-v2wlu5eq 13 1 ( ( -LRB- cord-310467-v2wlu5eq 13 2 Woode Woode NNP cord-310467-v2wlu5eq 13 3 , , , cord-310467-v2wlu5eq 13 4 1969 1969 CD cord-310467-v2wlu5eq 13 5 major major JJ cord-310467-v2wlu5eq 13 6 structural structural JJ cord-310467-v2wlu5eq 13 7 proteins protein NNS cord-310467-v2wlu5eq 13 8 : : : cord-310467-v2wlu5eq 13 9 the the DT cord-310467-v2wlu5eq 13 10 nucleocapsid nucleocapsid NN cord-310467-v2wlu5eq 13 11 ( ( -LRB- cord-310467-v2wlu5eq 13 12 N N NNP cord-310467-v2wlu5eq 13 13 ) ) -RRB- cord-310467-v2wlu5eq 13 14 , , , cord-310467-v2wlu5eq 13 15 which which WDT cord-310467-v2wlu5eq 13 16 is be VBZ cord-310467-v2wlu5eq 13 17 associated associate VBN cord-310467-v2wlu5eq 13 18 with with IN cord-310467-v2wlu5eq 13 19 the the DT cord-310467-v2wlu5eq 13 20 RNA RNA NNP cord-310467-v2wlu5eq 13 21 genome genome NN cord-310467-v2wlu5eq 13 22 ; ; : cord-310467-v2wlu5eq 13 23 the the DT cord-310467-v2wlu5eq 13 24 peplomer peplomer NNP cord-310467-v2wlu5eq 13 25 glycoprotein glycoprotein NN cord-310467-v2wlu5eq 13 26 ( ( -LRB- cord-310467-v2wlu5eq 13 27 S S NNP cord-310467-v2wlu5eq 13 28 ) ) -RRB- cord-310467-v2wlu5eq 13 29 and and CC cord-310467-v2wlu5eq 13 30 the the DT cord-310467-v2wlu5eq 13 31 transmembrane transmembrane NN cord-310467-v2wlu5eq 13 32 glycoprotein glycoprotein NN cord-310467-v2wlu5eq 13 33 ( ( -LRB- cord-310467-v2wlu5eq 13 34 M M NNP cord-310467-v2wlu5eq 13 35 ) ) -RRB- cord-310467-v2wlu5eq 13 36 ( ( -LRB- cord-310467-v2wlu5eq 13 37 Laude Laude NNP cord-310467-v2wlu5eq 13 38 et et FW cord-310467-v2wlu5eq 13 39 al al NNP cord-310467-v2wlu5eq 13 40 . . NNP cord-310467-v2wlu5eq 13 41 , , , cord-310467-v2wlu5eq 13 42 1990 1990 CD cord-310467-v2wlu5eq 13 43 ) ) -RRB- cord-310467-v2wlu5eq 13 44 . . . cord-310467-v2wlu5eq 14 1 In in IN cord-310467-v2wlu5eq 14 2 recent recent JJ cord-310467-v2wlu5eq 14 3 years year NNS cord-310467-v2wlu5eq 14 4 there there EX cord-310467-v2wlu5eq 14 5 has have VBZ cord-310467-v2wlu5eq 14 6 been be VBN cord-310467-v2wlu5eq 14 7 increasing increase VBG cord-310467-v2wlu5eq 14 8 interest interest NN cord-310467-v2wlu5eq 14 9 in in IN cord-310467-v2wlu5eq 14 10 the the DT cord-310467-v2wlu5eq 14 11 development development NN cord-310467-v2wlu5eq 14 12 and and CC cord-310467-v2wlu5eq 14 13 application application NN cord-310467-v2wlu5eq 14 14 of of IN cord-310467-v2wlu5eq 14 15 the the DT cord-310467-v2wlu5eq 14 16 fixed fix VBN cord-310467-v2wlu5eq 14 17 - - HYPH cord-310467-v2wlu5eq 14 18 cell cell NN cord-310467-v2wlu5eq 14 19 ELISA ELISA NNP cord-310467-v2wlu5eq 14 20 system system NN cord-310467-v2wlu5eq 14 21 for for IN cord-310467-v2wlu5eq 14 22 virological virological JJ cord-310467-v2wlu5eq 14 23 and and CC cord-310467-v2wlu5eq 14 24 immunological immunological JJ cord-310467-v2wlu5eq 14 25 investigations investigation NNS cord-310467-v2wlu5eq 14 26 . . . cord-310467-v2wlu5eq 15 1 For for IN cord-310467-v2wlu5eq 15 2 TGE TGE NNP cord-310467-v2wlu5eq 15 3 infections infection NNS cord-310467-v2wlu5eq 15 4 , , , cord-310467-v2wlu5eq 15 5 this this DT cord-310467-v2wlu5eq 15 6 technique technique NN cord-310467-v2wlu5eq 15 7 using use VBG cord-310467-v2wlu5eq 15 8 infected infected JJ cord-310467-v2wlu5eq 15 9 cells cell NNS cord-310467-v2wlu5eq 15 10 as as IN cord-310467-v2wlu5eq 15 11 a a DT cord-310467-v2wlu5eq 15 12 source source NN cord-310467-v2wlu5eq 15 13 of of IN cord-310467-v2wlu5eq 15 14 antigen antigen NN cord-310467-v2wlu5eq 15 15 has have VBZ cord-310467-v2wlu5eq 15 16 been be VBN cord-310467-v2wlu5eq 15 17 used use VBN cord-310467-v2wlu5eq 15 18 to to TO cord-310467-v2wlu5eq 15 19 detect detect VB cord-310467-v2wlu5eq 15 20 total total JJ cord-310467-v2wlu5eq 15 21 anti anti JJ cord-310467-v2wlu5eq 15 22 - - JJ cord-310467-v2wlu5eq 15 23 TGEV tgev JJ cord-310467-v2wlu5eq 15 24 antibodies antibody NNS cord-310467-v2wlu5eq 15 25 ( ( -LRB- cord-310467-v2wlu5eq 15 26 Kodama Kodama NNP cord-310467-v2wlu5eq 15 27 et et FW cord-310467-v2wlu5eq 15 28 al al NNP cord-310467-v2wlu5eq 15 29 . . NNP cord-310467-v2wlu5eq 15 30 , , , cord-310467-v2wlu5eq 15 31 1980a 1980a CD cord-310467-v2wlu5eq 15 32 ) ) -RRB- cord-310467-v2wlu5eq 15 33 or or CC cord-310467-v2wlu5eq 15 34 IgA IgA NNP cord-310467-v2wlu5eq 15 35 antibodies antibody NNS cord-310467-v2wlu5eq 15 36 ( ( -LRB- cord-310467-v2wlu5eq 15 37 Kodama Kodama NNP cord-310467-v2wlu5eq 15 38 et et FW cord-310467-v2wlu5eq 15 39 al al NNP cord-310467-v2wlu5eq 15 40 . . NNP cord-310467-v2wlu5eq 15 41 , , , cord-310467-v2wlu5eq 15 42 1980b 1980b CD cord-310467-v2wlu5eq 15 43 ) ) -RRB- cord-310467-v2wlu5eq 15 44 in in IN cord-310467-v2wlu5eq 15 45 the the DT cord-310467-v2wlu5eq 15 46 serum serum NN cord-310467-v2wlu5eq 15 47 of of IN cord-310467-v2wlu5eq 15 48 infected infected JJ cord-310467-v2wlu5eq 15 49 swine swine NN cord-310467-v2wlu5eq 15 50 . . . cord-310467-v2wlu5eq 16 1 For for IN cord-310467-v2wlu5eq 16 2 other other JJ cord-310467-v2wlu5eq 16 3 viruses virus NNS cord-310467-v2wlu5eq 16 4 , , , cord-310467-v2wlu5eq 16 5 it -PRON- PRP cord-310467-v2wlu5eq 16 6 has have VBZ cord-310467-v2wlu5eq 16 7 been be VBN cord-310467-v2wlu5eq 16 8 used use VBN cord-310467-v2wlu5eq 16 9 for for IN cord-310467-v2wlu5eq 16 10 screening screen VBG cord-310467-v2wlu5eq 16 11 hybridomas hybridomas NN cord-310467-v2wlu5eq 17 1 ( ( -LRB- cord-310467-v2wlu5eq 17 2 Russell Russell NNP cord-310467-v2wlu5eq 17 3 et et NNP cord-310467-v2wlu5eq 17 4 al al NNP cord-310467-v2wlu5eq 17 5 . . NNP cord-310467-v2wlu5eq 17 6 , , , cord-310467-v2wlu5eq 17 7 1983 1983 CD cord-310467-v2wlu5eq 17 8 ) ) -RRB- cord-310467-v2wlu5eq 17 9 , , , cord-310467-v2wlu5eq 17 10 for for IN cord-310467-v2wlu5eq 17 11 diagnosis diagnosis NN cord-310467-v2wlu5eq 18 1 ( ( -LRB- cord-310467-v2wlu5eq 18 2 Pauli Pauli NNP cord-310467-v2wlu5eq 18 3 et et NNP cord-310467-v2wlu5eq 18 4 al al NNP cord-310467-v2wlu5eq 18 5 . . NNP cord-310467-v2wlu5eq 18 6 , , , cord-310467-v2wlu5eq 18 7 1984 1984 CD cord-310467-v2wlu5eq 18 8 ; ; : cord-310467-v2wlu5eq 18 9 Groin Groin NNP cord-310467-v2wlu5eq 18 10 and and CC cord-310467-v2wlu5eq 18 11 Bernard Bernard NNP cord-310467-v2wlu5eq 18 12 , , , cord-310467-v2wlu5eq 18 13 1985 1985 CD cord-310467-v2wlu5eq 18 14 ; ; : cord-310467-v2wlu5eq 18 15 Van Van NNP cord-310467-v2wlu5eq 18 16 Tiel Tiel NNP cord-310467-v2wlu5eq 18 17 et et FW cord-310467-v2wlu5eq 18 18 al al NNP cord-310467-v2wlu5eq 18 19 . . NNP cord-310467-v2wlu5eq 18 20 , , , cord-310467-v2wlu5eq 18 21 1988 1988 CD cord-310467-v2wlu5eq 18 22 ) ) -RRB- cord-310467-v2wlu5eq 19 1 and and CC cord-310467-v2wlu5eq 19 2 for for IN cord-310467-v2wlu5eq 19 3 identification identification NN cord-310467-v2wlu5eq 19 4 of of IN cord-310467-v2wlu5eq 19 5 epitopes epitope NNS cord-310467-v2wlu5eq 19 6 on on IN cord-310467-v2wlu5eq 19 7 enveloped envelop VBN cord-310467-v2wlu5eq 19 8 or or CC cord-310467-v2wlu5eq 19 9 naked naked JJ cord-310467-v2wlu5eq 19 10 viruses virus NNS cord-310467-v2wlu5eq 19 11 ( ( -LRB- cord-310467-v2wlu5eq 19 12 Anderson Anderson NNP cord-310467-v2wlu5eq 19 13 et et NNP cord-310467-v2wlu5eq 19 14 al al NNP cord-310467-v2wlu5eq 19 15 . . NNP cord-310467-v2wlu5eq 19 16 , , , cord-310467-v2wlu5eq 19 17 1986 1986 CD cord-310467-v2wlu5eq 19 18 ; ; : cord-310467-v2wlu5eq 19 19 Boere Boere NNP cord-310467-v2wlu5eq 19 20 et et FW cord-310467-v2wlu5eq 19 21 al al NNP cord-310467-v2wlu5eq 19 22 . . NNP cord-310467-v2wlu5eq 19 23 , , , cord-310467-v2wlu5eq 19 24 1986 1986 CD cord-310467-v2wlu5eq 19 25 ; ; : cord-310467-v2wlu5eq 19 26 Oosterlaken Oosterlaken NNP cord-310467-v2wlu5eq 19 27 et et NNP cord-310467-v2wlu5eq 19 28 ai ai NNP cord-310467-v2wlu5eq 19 29 . . NNP cord-310467-v2wlu5eq 19 30 , , , cord-310467-v2wlu5eq 19 31 1989 1989 CD cord-310467-v2wlu5eq 19 32 ; ; : cord-310467-v2wlu5eq 19 33 Simkins Simkins NNP cord-310467-v2wlu5eq 19 34 et et NNP cord-310467-v2wlu5eq 19 35 al al NNP cord-310467-v2wlu5eq 19 36 . . NNP cord-310467-v2wlu5eq 19 37 , , , cord-310467-v2wlu5eq 19 38 1989 1989 CD cord-310467-v2wlu5eq 19 39 ) ) -RRB- cord-310467-v2wlu5eq 19 40 . . . cord-310467-v2wlu5eq 20 1 Recently recently RB cord-310467-v2wlu5eq 20 2 , , , cord-310467-v2wlu5eq 20 3 we -PRON- PRP cord-310467-v2wlu5eq 20 4 described describe VBD cord-310467-v2wlu5eq 20 5 a a DT cord-310467-v2wlu5eq 20 6 quantitative quantitative JJ cord-310467-v2wlu5eq 20 7 fixed fix VBN cord-310467-v2wlu5eq 20 8 - - HYPH cord-310467-v2wlu5eq 20 9 cell cell NN cord-310467-v2wlu5eq 20 10 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 20 11 technique technique NN cord-310467-v2wlu5eq 20 12 for for IN cord-310467-v2wlu5eq 20 13 the the DT cord-310467-v2wlu5eq 20 14 study study NN cord-310467-v2wlu5eq 20 15 of of IN cord-310467-v2wlu5eq 20 16 surface surface NN cord-310467-v2wlu5eq 20 17 viral viral JJ cord-310467-v2wlu5eq 20 18 antigens antigen NNS cord-310467-v2wlu5eq 20 19 induced induce VBN cord-310467-v2wlu5eq 20 20 by by IN cord-310467-v2wlu5eq 20 21 TGEV TGEV NNP cord-310467-v2wlu5eq 20 22 ( ( -LRB- cord-310467-v2wlu5eq 20 23 I"6 I"6 NNP cord-310467-v2wlu5eq 20 24 et et NNP cord-310467-v2wlu5eq 20 25 al al NNP cord-310467-v2wlu5eq 20 26 . . NNP cord-310467-v2wlu5eq 20 27 , , , cord-310467-v2wlu5eq 20 28 1991 1991 CD cord-310467-v2wlu5eq 20 29 ) ) -RRB- cord-310467-v2wlu5eq 20 30 . . . cord-310467-v2wlu5eq 21 1 As as IN cord-310467-v2wlu5eq 21 2 the the DT cord-310467-v2wlu5eq 21 3 name name NN cord-310467-v2wlu5eq 21 4 of of IN cord-310467-v2wlu5eq 21 5 the the DT cord-310467-v2wlu5eq 21 6 technique technique NN cord-310467-v2wlu5eq 21 7 implies imply VBZ cord-310467-v2wlu5eq 21 8 , , , cord-310467-v2wlu5eq 21 9 many many JJ cord-310467-v2wlu5eq 21 10 fixatives fixative NNS cord-310467-v2wlu5eq 21 11 have have VBP cord-310467-v2wlu5eq 21 12 been be VBN cord-310467-v2wlu5eq 21 13 used use VBN cord-310467-v2wlu5eq 21 14 by by IN cord-310467-v2wlu5eq 21 15 different different JJ cord-310467-v2wlu5eq 21 16 authors author NNS cord-310467-v2wlu5eq 21 17 to to TO cord-310467-v2wlu5eq 21 18 fix fix VB cord-310467-v2wlu5eq 21 19 infected infected JJ cord-310467-v2wlu5eq 21 20 cells cell NNS cord-310467-v2wlu5eq 21 21 to to IN cord-310467-v2wlu5eq 21 22 the the DT cord-310467-v2wlu5eq 21 23 plastic plastic JJ cord-310467-v2wlu5eq 21 24 plates plate NNS cord-310467-v2wlu5eq 21 25 . . . cord-310467-v2wlu5eq 22 1 However however RB cord-310467-v2wlu5eq 22 2 , , , cord-310467-v2wlu5eq 22 3 there there EX cord-310467-v2wlu5eq 22 4 have have VBP cord-310467-v2wlu5eq 22 5 not not RB cord-310467-v2wlu5eq 22 6 been be VBN cord-310467-v2wlu5eq 22 7 any any DT cord-310467-v2wlu5eq 22 8 published publish VBN cord-310467-v2wlu5eq 22 9 reports report NNS cord-310467-v2wlu5eq 22 10 concerning concern VBG cord-310467-v2wlu5eq 22 11 the the DT cord-310467-v2wlu5eq 22 12 effect effect NN cord-310467-v2wlu5eq 22 13 of of IN cord-310467-v2wlu5eq 22 14 fixatives fixative NNS cord-310467-v2wlu5eq 22 15 on on IN cord-310467-v2wlu5eq 22 16 the the DT cord-310467-v2wlu5eq 22 17 detection detection NN cord-310467-v2wlu5eq 22 18 of of IN cord-310467-v2wlu5eq 22 19 viral viral JJ cord-310467-v2wlu5eq 22 20 antigens antigen NNS cord-310467-v2wlu5eq 22 21 in in IN cord-310467-v2wlu5eq 22 22 infected infected JJ cord-310467-v2wlu5eq 22 23 cells cell NNS cord-310467-v2wlu5eq 22 24 by by IN cord-310467-v2wlu5eq 22 25 this this DT cord-310467-v2wlu5eq 22 26 technique technique NN cord-310467-v2wlu5eq 22 27 . . . cord-310467-v2wlu5eq 23 1 In in IN cord-310467-v2wlu5eq 23 2 the the DT cord-310467-v2wlu5eq 23 3 present present JJ cord-310467-v2wlu5eq 23 4 study study NN cord-310467-v2wlu5eq 23 5 , , , cord-310467-v2wlu5eq 23 6 we -PRON- PRP cord-310467-v2wlu5eq 23 7 have have VBP cord-310467-v2wlu5eq 23 8 established establish VBN cord-310467-v2wlu5eq 23 9 the the DT cord-310467-v2wlu5eq 23 10 effect effect NN cord-310467-v2wlu5eq 23 11 of of IN cord-310467-v2wlu5eq 23 12 various various JJ cord-310467-v2wlu5eq 23 13 fixatives fixative NNS cord-310467-v2wlu5eq 23 14 and and CC cord-310467-v2wlu5eq 23 15 detergents detergent NNS cord-310467-v2wlu5eq 23 16 on on IN cord-310467-v2wlu5eq 23 17 the the DT cord-310467-v2wlu5eq 23 18 detection detection NN cord-310467-v2wlu5eq 23 19 of of IN cord-310467-v2wlu5eq 23 20 TGEV TGEV NNP cord-310467-v2wlu5eq 23 21 determinants determinant NNS cord-310467-v2wlu5eq 23 22 in in IN cord-310467-v2wlu5eq 23 23 infected infected JJ cord-310467-v2wlu5eq 23 24 cells cell NNS cord-310467-v2wlu5eq 23 25 , , , cord-310467-v2wlu5eq 23 26 by by IN cord-310467-v2wlu5eq 23 27 means mean NNS cord-310467-v2wlu5eq 23 28 of of IN cord-310467-v2wlu5eq 23 29 MAbs MAbs NNP cord-310467-v2wlu5eq 23 30 or or CC cord-310467-v2wlu5eq 23 31 PAbs PAbs NNP cord-310467-v2wlu5eq 23 32 in in IN cord-310467-v2wlu5eq 23 33 a a DT cord-310467-v2wlu5eq 23 34 quantitative quantitative JJ cord-310467-v2wlu5eq 23 35 fixed fix VBN cord-310467-v2wlu5eq 23 36 - - HYPH cord-310467-v2wlu5eq 23 37 cell cell NN cord-310467-v2wlu5eq 23 38 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 23 39 technique technique NN cord-310467-v2wlu5eq 23 40 ( ( -LRB- cord-310467-v2wlu5eq 23 41 IPT IPT NNP cord-310467-v2wlu5eq 23 42 ) ) -RRB- cord-310467-v2wlu5eq 23 43 , , , cord-310467-v2wlu5eq 23 44 The the DT cord-310467-v2wlu5eq 23 45 high high RB cord-310467-v2wlu5eq 23 46 - - HYPH cord-310467-v2wlu5eq 23 47 passaged passage VBN cord-310467-v2wlu5eq 23 48 Purdue Purdue NNP cord-310467-v2wlu5eq 23 49 - - HYPH cord-310467-v2wlu5eq 23 50 ll5 ll5 NNP cord-310467-v2wlu5eq 23 51 strain strain NN cord-310467-v2wlu5eq 23 52 of of IN cord-310467-v2wlu5eq 23 53 TGEV TGEV NNP cord-310467-v2wlu5eq 23 54 ( ( -LRB- cord-310467-v2wlu5eq 24 1 Bohl Bohl NNP cord-310467-v2wlu5eq 24 2 et et FW cord-310467-v2wlu5eq 24 3 al al NNP cord-310467-v2wlu5eq 24 4 . . NNP cord-310467-v2wlu5eq 24 5 , , , cord-310467-v2wlu5eq 24 6 1972 1972 CD cord-310467-v2wlu5eq 24 7 ) ) -RRB- cord-310467-v2wlu5eq 24 8 and and CC cord-310467-v2wlu5eq 24 9 the the DT cord-310467-v2wlu5eq 24 10 swine swine NN cord-310467-v2wlu5eq 24 11 testis testis NN cord-310467-v2wlu5eq 24 12 ( ( -LRB- cord-310467-v2wlu5eq 24 13 ST ST NNP cord-310467-v2wlu5eq 24 14 ) ) -RRB- cord-310467-v2wlu5eq 24 15 cell cell NN cord-310467-v2wlu5eq 24 16 line line NN cord-310467-v2wlu5eq 24 17 ( ( -LRB- cord-310467-v2wlu5eq 24 18 McClurkin McClurkin NNP cord-310467-v2wlu5eq 24 19 and and CC cord-310467-v2wlu5eq 24 20 Norman Norman NNP cord-310467-v2wlu5eq 24 21 , , , cord-310467-v2wlu5eq 24 22 1966 1966 CD cord-310467-v2wlu5eq 24 23 ) ) -RRB- cord-310467-v2wlu5eq 24 24 , , , cord-310467-v2wlu5eq 24 25 supplied supply VBN cord-310467-v2wlu5eq 24 26 by by IN cord-310467-v2wlu5eq 24 27 Dr. Dr. NNP cord-310467-v2wlu5eq 24 28 E.H. E.H. NNP cord-310467-v2wlu5eq 24 29 Bohl Bohl NNP cord-310467-v2wlu5eq 24 30 ( ( -LRB- cord-310467-v2wlu5eq 24 31 Wooster Wooster NNP cord-310467-v2wlu5eq 24 32 , , , cord-310467-v2wlu5eq 24 33 OH oh UH cord-310467-v2wlu5eq 24 34 , , , cord-310467-v2wlu5eq 24 35 USA USA NNP cord-310467-v2wlu5eq 24 36 ) ) -RRB- cord-310467-v2wlu5eq 24 37 were be VBD cord-310467-v2wlu5eq 24 38 used use VBN cord-310467-v2wlu5eq 24 39 in in IN cord-310467-v2wlu5eq 24 40 this this DT cord-310467-v2wlu5eq 24 41 study study NN cord-310467-v2wlu5eq 24 42 . . . cord-310467-v2wlu5eq 25 1 Eagle Eagle NNP cord-310467-v2wlu5eq 25 2 's 's POS cord-310467-v2wlu5eq 25 3 minimal minimal JJ cord-310467-v2wlu5eq 25 4 essential essential JJ cord-310467-v2wlu5eq 25 5 medium medium NN cord-310467-v2wlu5eq 25 6 ( ( -LRB- cord-310467-v2wlu5eq 25 7 EMEM EMEM NNP cord-310467-v2wlu5eq 25 8 ) ) -RRB- cord-310467-v2wlu5eq 25 9 supplemented supplement VBD cord-310467-v2wlu5eq 25 10 with with IN cord-310467-v2wlu5eq 25 11 10 10 CD cord-310467-v2wlu5eq 25 12 % % NN cord-310467-v2wlu5eq 25 13 heat heat NN cord-310467-v2wlu5eq 25 14 - - HYPH cord-310467-v2wlu5eq 25 15 inactivated inactivate VBN cord-310467-v2wlu5eq 25 16 fetal fetal JJ cord-310467-v2wlu5eq 25 17 calf calf NN cord-310467-v2wlu5eq 25 18 serum serum NN cord-310467-v2wlu5eq 25 19 ( ( -LRB- cord-310467-v2wlu5eq 25 20 FCS FCS NNP cord-310467-v2wlu5eq 25 21 ) ) -RRB- cord-310467-v2wlu5eq 25 22 , , , cord-310467-v2wlu5eq 25 23 penicillin penicillin NN cord-310467-v2wlu5eq 25 24 ( ( -LRB- cord-310467-v2wlu5eq 25 25 100 100 CD cord-310467-v2wlu5eq 25 26 IU IU NNP cord-310467-v2wlu5eq 25 27 / / SYM cord-310467-v2wlu5eq 25 28 ml ml NN cord-310467-v2wlu5eq 25 29 ) ) -RRB- cord-310467-v2wlu5eq 25 30 and and CC cord-310467-v2wlu5eq 25 31 streptomycin streptomycin NNS cord-310467-v2wlu5eq 25 32 ( ( -LRB- cord-310467-v2wlu5eq 25 33 100 100 CD cord-310467-v2wlu5eq 25 34 / / SYM cord-310467-v2wlu5eq 25 35 tg tg NNP cord-310467-v2wlu5eq 25 36 / / SYM cord-310467-v2wlu5eq 25 37 ml ml NNP cord-310467-v2wlu5eq 25 38 ) ) -RRB- cord-310467-v2wlu5eq 25 39 was be VBD cord-310467-v2wlu5eq 25 40 used use VBN cord-310467-v2wlu5eq 25 41 for for IN cord-310467-v2wlu5eq 25 42 cell cell NN cord-310467-v2wlu5eq 25 43 growth growth NN cord-310467-v2wlu5eq 25 44 . . . cord-310467-v2wlu5eq 26 1 Confluent confluent NN cord-310467-v2wlu5eq 26 2 monolayers monolayer NNS cord-310467-v2wlu5eq 26 3 of of IN cord-310467-v2wlu5eq 26 4 2.5 2.5 CD cord-310467-v2wlu5eq 26 5 × × NN cord-310467-v2wlu5eq 26 6 105 105 CD cord-310467-v2wlu5eq 26 7 ceUs ceUs NNP cord-310467-v2wlu5eq 26 8 / / SYM cord-310467-v2wlu5eq 26 9 cm cm NNP cord-310467-v2wlu5eq 26 10 2 2 CD cord-310467-v2wlu5eq 26 11 in in IN cord-310467-v2wlu5eq 26 12 96-well 96-well CD cord-310467-v2wlu5eq 26 13 , , , cord-310467-v2wlu5eq 26 14 flat flat RB cord-310467-v2wlu5eq 26 15 - - HYPH cord-310467-v2wlu5eq 26 16 bottomed bottomed JJ cord-310467-v2wlu5eq 26 17 plastic plastic NN cord-310467-v2wlu5eq 26 18 plates plate NNS cord-310467-v2wlu5eq 26 19 ( ( -LRB- cord-310467-v2wlu5eq 26 20 Falcon Falcon NNP cord-310467-v2wlu5eq 26 21 3072 3072 CD cord-310467-v2wlu5eq 26 22 , , , cord-310467-v2wlu5eq 26 23 Becton Becton NNP cord-310467-v2wlu5eq 26 24 Dickinson Dickinson NNP cord-310467-v2wlu5eq 26 25 ) ) -RRB- cord-310467-v2wlu5eq 26 26 were be VBD cord-310467-v2wlu5eq 26 27 inoculated inoculate VBN cord-310467-v2wlu5eq 26 28 with with IN cord-310467-v2wlu5eq 26 29 a a DT cord-310467-v2wlu5eq 26 30 volume volume NN cord-310467-v2wlu5eq 26 31 of of IN cord-310467-v2wlu5eq 26 32 0.1 0.1 CD cord-310467-v2wlu5eq 26 33 ml ml NN cord-310467-v2wlu5eq 26 34 / / SYM cord-310467-v2wlu5eq 26 35 well well RB cord-310467-v2wlu5eq 26 36 of of IN cord-310467-v2wlu5eq 26 37 virus virus NN cord-310467-v2wlu5eq 26 38 suspension suspension NN cord-310467-v2wlu5eq 26 39 at at IN cord-310467-v2wlu5eq 26 40 a a DT cord-310467-v2wlu5eq 26 41 multiplicity multiplicity NN cord-310467-v2wlu5eq 26 42 of of IN cord-310467-v2wlu5eq 26 43 infection infection NN cord-310467-v2wlu5eq 26 44 ( ( -LRB- cord-310467-v2wlu5eq 26 45 m.o.i m.o.i NNP cord-310467-v2wlu5eq 26 46 ) ) -RRB- cord-310467-v2wlu5eq 26 47 of of IN cord-310467-v2wlu5eq 26 48 1 1 CD cord-310467-v2wlu5eq 26 49 . . . cord-310467-v2wlu5eq 27 1 After after IN cord-310467-v2wlu5eq 27 2 30 30 CD cord-310467-v2wlu5eq 27 3 rain rain NN cord-310467-v2wlu5eq 27 4 of of IN cord-310467-v2wlu5eq 27 5 incubation incubation NN cord-310467-v2wlu5eq 27 6 at at IN cord-310467-v2wlu5eq 27 7 37 37 CD cord-310467-v2wlu5eq 27 8 ° ° NN cord-310467-v2wlu5eq 27 9 C c NN cord-310467-v2wlu5eq 27 10 under under IN cord-310467-v2wlu5eq 27 11 5.5 5.5 CD cord-310467-v2wlu5eq 27 12 % % NN cord-310467-v2wlu5eq 27 13 CO co NN cord-310467-v2wlu5eq 27 14 2 2 CD cord-310467-v2wlu5eq 27 15 , , , cord-310467-v2wlu5eq 27 16 the the DT cord-310467-v2wlu5eq 27 17 inoculum inoculum NN cord-310467-v2wlu5eq 27 18 in in IN cord-310467-v2wlu5eq 27 19 each each DT cord-310467-v2wlu5eq 27 20 well well NN cord-310467-v2wlu5eq 27 21 was be VBD cord-310467-v2wlu5eq 27 22 removed remove VBN cord-310467-v2wlu5eq 27 23 , , , cord-310467-v2wlu5eq 27 24 washed wash VBN cord-310467-v2wlu5eq 27 25 twice twice RB cord-310467-v2wlu5eq 27 26 with with IN cord-310467-v2wlu5eq 27 27 phosphate phosphate NN cord-310467-v2wlu5eq 27 28 buffered buffer VBN cord-310467-v2wlu5eq 27 29 saline saline NN cord-310467-v2wlu5eq 27 30 ( ( -LRB- cord-310467-v2wlu5eq 27 31 PBS PBS NNP cord-310467-v2wlu5eq 27 32 ) ) -RRB- cord-310467-v2wlu5eq 27 33 and and CC cord-310467-v2wlu5eq 27 34 replaced replace VBD cord-310467-v2wlu5eq 27 35 with with IN cord-310467-v2wlu5eq 27 36 0.1 0.1 CD cord-310467-v2wlu5eq 27 37 ml ml NN cord-310467-v2wlu5eq 27 38 / / SYM cord-310467-v2wlu5eq 27 39 well well NN cord-310467-v2wlu5eq 27 40 of of IN cord-310467-v2wlu5eq 27 41 EMEM EMEM NNP cord-310467-v2wlu5eq 27 42 containing contain VBG cord-310467-v2wlu5eq 27 43 5 5 CD cord-310467-v2wlu5eq 27 44 % % NN cord-310467-v2wlu5eq 27 45 heatinactivated heatinactivate VBN cord-310467-v2wlu5eq 27 46 FCS FCS NNP cord-310467-v2wlu5eq 27 47 . . . cord-310467-v2wlu5eq 28 1 The the DT cord-310467-v2wlu5eq 28 2 infected infected JJ cord-310467-v2wlu5eq 28 3 cells cell NNS cord-310467-v2wlu5eq 28 4 were be VBD cord-310467-v2wlu5eq 28 5 incubated incubate VBN cord-310467-v2wlu5eq 28 6 at at IN cord-310467-v2wlu5eq 28 7 37 37 CD cord-310467-v2wlu5eq 28 8 ° ° NN cord-310467-v2wlu5eq 28 9 C c NN cord-310467-v2wlu5eq 28 10 under under IN cord-310467-v2wlu5eq 28 11 5.5 5.5 CD cord-310467-v2wlu5eq 28 12 % % NN cord-310467-v2wlu5eq 28 13 CO co NN cord-310467-v2wlu5eq 28 14 2 2 CD cord-310467-v2wlu5eq 28 15 for for IN cord-310467-v2wlu5eq 28 16 an an DT cord-310467-v2wlu5eq 28 17 additional additional JJ cord-310467-v2wlu5eq 28 18 15 15 CD cord-310467-v2wlu5eq 28 19 h h NN cord-310467-v2wlu5eq 28 20 , , , cord-310467-v2wlu5eq 28 21 The the DT cord-310467-v2wlu5eq 28 22 unfixed unfixed JJ cord-310467-v2wlu5eq 28 23 , , , cord-310467-v2wlu5eq 28 24 TGEV TGEV NNP cord-310467-v2wlu5eq 28 25 - - HYPH cord-310467-v2wlu5eq 28 26 infecmd infecmd NNP cord-310467-v2wlu5eq 28 27 cells cell NNS cord-310467-v2wlu5eq 28 28 served serve VBD cord-310467-v2wlu5eq 28 29 as as IN cord-310467-v2wlu5eq 28 30 controls control NNS cord-310467-v2wlu5eq 28 31 . . . cord-310467-v2wlu5eq 29 1 The the DT cord-310467-v2wlu5eq 29 2 MAbs MAbs NNP cord-310467-v2wlu5eq 29 3 were be VBD cord-310467-v2wlu5eq 29 4 directed direct VBN cord-310467-v2wlu5eq 29 5 against against IN cord-310467-v2wlu5eq 29 6 M M NNP cord-310467-v2wlu5eq 29 7 ( ( -LRB- cord-310467-v2wlu5eq 29 8 25 25 CD cord-310467-v2wlu5eq 29 9 - - SYM cord-310467-v2wlu5eq 29 10 22 22 CD cord-310467-v2wlu5eq 29 11 ) ) -RRB- cord-310467-v2wlu5eq 29 12 , , , cord-310467-v2wlu5eq 29 13 S S NNP cord-310467-v2wlu5eq 29 14 ( ( -LRB- cord-310467-v2wlu5eq 29 15 51 51 CD cord-310467-v2wlu5eq 29 16 - - SYM cord-310467-v2wlu5eq 29 17 13 13 CD cord-310467-v2wlu5eq 29 18 ) ) -RRB- cord-310467-v2wlu5eq 29 19 and and CC cord-310467-v2wlu5eq 29 20 N N NNP cord-310467-v2wlu5eq 29 21 ( ( -LRB- cord-310467-v2wlu5eq 29 22 22 22 CD cord-310467-v2wlu5eq 29 23 - - SYM cord-310467-v2wlu5eq 29 24 6 6 CD cord-310467-v2wlu5eq 29 25 ) ) -RRB- cord-310467-v2wlu5eq 29 26 determinants determinant NNS cord-310467-v2wlu5eq 29 27 of of IN cord-310467-v2wlu5eq 29 28 TGEV TGEV NNS cord-310467-v2wlu5eq 29 29 and and CC cord-310467-v2wlu5eq 29 30 had have VBD cord-310467-v2wlu5eq 29 31 been be VBN cord-310467-v2wlu5eq 29 32 prepared prepare VBN cord-310467-v2wlu5eq 29 33 and and CC cord-310467-v2wlu5eq 29 34 used use VBN cord-310467-v2wlu5eq 29 35 as as IN cord-310467-v2wlu5eq 29 36 ascitic ascitic JJ cord-310467-v2wlu5eq 29 37 fluids fluid NNS cord-310467-v2wlu5eq 29 38 following follow VBG cord-310467-v2wlu5eq 29 39 injection injection NN cord-310467-v2wlu5eq 29 40 of of IN cord-310467-v2wlu5eq 29 41 BALB BALB NNP cord-310467-v2wlu5eq 29 42 / / SYM cord-310467-v2wlu5eq 29 43 c c NN cord-310467-v2wlu5eq 29 44 mice mouse NNS cord-310467-v2wlu5eq 29 45 with with IN cord-310467-v2wlu5eq 29 46 the the DT cord-310467-v2wlu5eq 29 47 antibody antibody NN cord-310467-v2wlu5eq 29 48 - - HYPH cord-310467-v2wlu5eq 29 49 producing produce VBG cord-310467-v2wlu5eq 29 50 hybridomas hybridomas NN cord-310467-v2wlu5eq 30 1 ( ( -LRB- cord-310467-v2wlu5eq 30 2 Laude Laude NNP cord-310467-v2wlu5eq 30 3 et et NNP cord-310467-v2wlu5eq 30 4 al al NNP cord-310467-v2wlu5eq 30 5 . . NNP cord-310467-v2wlu5eq 30 6 , , , cord-310467-v2wlu5eq 30 7 1986 1986 CD cord-310467-v2wlu5eq 30 8 ) ) -RRB- cord-310467-v2wlu5eq 30 9 . . . cord-310467-v2wlu5eq 31 1 The the DT cord-310467-v2wlu5eq 31 2 anti anti JJ cord-310467-v2wlu5eq 31 3 - - JJ cord-310467-v2wlu5eq 31 4 TGEV tgev JJ cord-310467-v2wlu5eq 31 5 PAbs PAbs NNP cord-310467-v2wlu5eq 31 6 ( ( -LRB- cord-310467-v2wlu5eq 31 7 53 53 CD cord-310467-v2wlu5eq 31 8 - - SYM cord-310467-v2wlu5eq 31 9 12 12 CD cord-310467-v2wlu5eq 31 10 ) ) -RRB- cord-310467-v2wlu5eq 31 11 obtained obtain VBD cord-310467-v2wlu5eq 31 12 from from IN cord-310467-v2wlu5eq 31 13 the the DT cord-310467-v2wlu5eq 31 14 serum serum NN cord-310467-v2wlu5eq 31 15 of of IN cord-310467-v2wlu5eq 31 16 a a DT cord-310467-v2wlu5eq 31 17 sow sow NN cord-310467-v2wlu5eq 31 18 orally orally RB cord-310467-v2wlu5eq 31 19 immunized immunize VBN cord-310467-v2wlu5eq 31 20 with with IN cord-310467-v2wlu5eq 31 21 vaccine vaccine NN cord-310467-v2wlu5eq 31 22 containing contain VBG cord-310467-v2wlu5eq 31 23 the the DT cord-310467-v2wlu5eq 31 24 Nouzilly Nouzilly NNP cord-310467-v2wlu5eq 31 25 strain strain NN cord-310467-v2wlu5eq 31 26 of of IN cord-310467-v2wlu5eq 31 27 TGEV TGEV NNP cord-310467-v2wlu5eq 31 28 ( ( -LRB- cord-310467-v2wlu5eq 31 29 Aynaud Aynaud NNP cord-310467-v2wlu5eq 31 30 et et FW cord-310467-v2wlu5eq 31 31 al al NNP cord-310467-v2wlu5eq 31 32 . . NNP cord-310467-v2wlu5eq 31 33 , , , cord-310467-v2wlu5eq 31 34 1985 1985 CD cord-310467-v2wlu5eq 31 35 ) ) -RRB- cord-310467-v2wlu5eq 31 36 , , , cord-310467-v2wlu5eq 31 37 and and CC cord-310467-v2wlu5eq 31 38 having have VBG cord-310467-v2wlu5eq 31 39 a a DT cord-310467-v2wlu5eq 31 40 neutralizing neutralize VBG cord-310467-v2wlu5eq 31 41 titer titer NN cord-310467-v2wlu5eq 31 42 of of IN cord-310467-v2wlu5eq 31 43 1:65,000 1:65,000 CD cord-310467-v2wlu5eq 31 44 , , , cord-310467-v2wlu5eq 31 45 were be VBD cord-310467-v2wlu5eq 31 46 used use VBN cord-310467-v2wlu5eq 31 47 for for IN cord-310467-v2wlu5eq 31 48 comparative comparative JJ cord-310467-v2wlu5eq 31 49 purposes purpose NNS cord-310467-v2wlu5eq 31 50 . . . cord-310467-v2wlu5eq 32 1 ( ( -LRB- cord-310467-v2wlu5eq 32 2 Pauli Pauli NNP cord-310467-v2wlu5eq 32 3 et et NNP cord-310467-v2wlu5eq 32 4 al al NNP cord-310467-v2wlu5eq 32 5 . . NNP cord-310467-v2wlu5eq 32 6 , , , cord-310467-v2wlu5eq 32 7 1984 1984 CD cord-310467-v2wlu5eq 32 8 ) ) -RRB- cord-310467-v2wlu5eq 32 9 , , , cord-310467-v2wlu5eq 32 10 60 60 CD cord-310467-v2wlu5eq 32 11 % % NN cord-310467-v2wlu5eq 32 12 acetone acetone NN cord-310467-v2wlu5eq 32 13 , , , cord-310467-v2wlu5eq 32 14 25 25 CD cord-310467-v2wlu5eq 32 15 % % NN cord-310467-v2wlu5eq 32 16 ethanol ethanol NN cord-310467-v2wlu5eq 32 17 and and CC cord-310467-v2wlu5eq 32 18 15 15 CD cord-310467-v2wlu5eq 32 19 % % NN cord-310467-v2wlu5eq 32 20 methanol methanol NN cord-310467-v2wlu5eq 32 21 ( ( -LRB- cord-310467-v2wlu5eq 32 22 Pauli Pauli NNP cord-310467-v2wlu5eq 32 23 et et NNP cord-310467-v2wlu5eq 32 24 al al NNP cord-310467-v2wlu5eq 32 25 . . NNP cord-310467-v2wlu5eq 32 26 , , , cord-310467-v2wlu5eq 32 27 1984 1984 CD cord-310467-v2wlu5eq 32 28 ) ) -RRB- cord-310467-v2wlu5eq 32 29 , , , cord-310467-v2wlu5eq 32 30 70 70 CD cord-310467-v2wlu5eq 32 31 % % NN cord-310467-v2wlu5eq 32 32 acetone acetone NN cord-310467-v2wlu5eq 32 33 and and CC cord-310467-v2wlu5eq 32 34 30 30 CD cord-310467-v2wlu5eq 32 35 % % NN cord-310467-v2wlu5eq 32 36 ethanol ethanol NN cord-310467-v2wlu5eq 32 37 ( ( -LRB- cord-310467-v2wlu5eq 32 38 Laude Laude NNP cord-310467-v2wlu5eq 32 39 et et FW cord-310467-v2wlu5eq 32 40 al al NNP cord-310467-v2wlu5eq 32 41 . . NNP cord-310467-v2wlu5eq 32 42 , , , cord-310467-v2wlu5eq 32 43 1986 1986 CD cord-310467-v2wlu5eq 32 44 ) ) -RRB- cord-310467-v2wlu5eq 32 45 . . . cord-310467-v2wlu5eq 33 1 Fixation fixation NN cord-310467-v2wlu5eq 33 2 procedure procedure NN cord-310467-v2wlu5eq 34 1 The the DT cord-310467-v2wlu5eq 34 2 TGEV TGEV NNP cord-310467-v2wlu5eq 34 3 - - HYPH cord-310467-v2wlu5eq 34 4 infected infect VBN cord-310467-v2wlu5eq 34 5 cells cell NNS cord-310467-v2wlu5eq 34 6 were be VBD cord-310467-v2wlu5eq 34 7 gently gently RB cord-310467-v2wlu5eq 34 8 washed wash VBN cord-310467-v2wlu5eq 34 9 twice twice RB cord-310467-v2wlu5eq 34 10 with with IN cord-310467-v2wlu5eq 34 11 PBS PBS NNP cord-310467-v2wlu5eq 34 12 and and CC cord-310467-v2wlu5eq 34 13 the the DT cord-310467-v2wlu5eq 34 14 cells cell NNS cord-310467-v2wlu5eq 34 15 were be VBD cord-310467-v2wlu5eq 34 16 fixed fix VBN cord-310467-v2wlu5eq 34 17 at at IN cord-310467-v2wlu5eq 34 18 -20 -20 NNP cord-310467-v2wlu5eq 34 19 ° ° NN cord-310467-v2wlu5eq 34 20 C C NNP cord-310467-v2wlu5eq 34 21 for for IN cord-310467-v2wlu5eq 34 22 30 30 CD cord-310467-v2wlu5eq 34 23 min min NN cord-310467-v2wlu5eq 34 24 with with IN cord-310467-v2wlu5eq 34 25 the the DT cord-310467-v2wlu5eq 34 26 appropriate appropriate JJ cord-310467-v2wlu5eq 34 27 fixative fixative NN cord-310467-v2wlu5eq 34 28 ( ( -LRB- cord-310467-v2wlu5eq 34 29 0.2 0.2 CD cord-310467-v2wlu5eq 34 30 ml ml NNS cord-310467-v2wlu5eq 34 31 per per IN cord-310467-v2wlu5eq 34 32 well well NN cord-310467-v2wlu5eq 34 33 ) ) -RRB- cord-310467-v2wlu5eq 34 34 which which WDT cord-310467-v2wlu5eq 34 35 was be VBD cord-310467-v2wlu5eq 34 36 previously previously RB cord-310467-v2wlu5eq 34 37 frozen freeze VBN cord-310467-v2wlu5eq 34 38 at at IN cord-310467-v2wlu5eq 34 39 -20 -20 NNP cord-310467-v2wlu5eq 34 40 ° ° NN cord-310467-v2wlu5eq 34 41 C C NNP cord-310467-v2wlu5eq 34 42 unless unless IN cord-310467-v2wlu5eq 34 43 otherwise otherwise RB cord-310467-v2wlu5eq 34 44 stated state VBN cord-310467-v2wlu5eq 34 45 . . . cord-310467-v2wlu5eq 35 1 The the DT cord-310467-v2wlu5eq 35 2 fixed fix VBN cord-310467-v2wlu5eq 35 3 cells cell NNS cord-310467-v2wlu5eq 35 4 were be VBD cord-310467-v2wlu5eq 35 5 washed wash VBN cord-310467-v2wlu5eq 35 6 twice twice RB cord-310467-v2wlu5eq 35 7 with with IN cord-310467-v2wlu5eq 35 8 PBS PBS NNP cord-310467-v2wlu5eq 35 9 before before IN cord-310467-v2wlu5eq 35 10 addition addition NN cord-310467-v2wlu5eq 35 11 of of IN cord-310467-v2wlu5eq 35 12 0.1 0.1 CD cord-310467-v2wlu5eq 35 13 ml/ ml/ XX cord-310467-v2wlu5eq 36 1 well well UH cord-310467-v2wlu5eq 36 2 of of IN cord-310467-v2wlu5eq 36 3 blocking block VBG cord-310467-v2wlu5eq 36 4 solution solution NN cord-310467-v2wlu5eq 36 5 and and CC cord-310467-v2wlu5eq 36 6 incubation incubation NN cord-310467-v2wlu5eq 36 7 for for IN cord-310467-v2wlu5eq 36 8 15 15 CD cord-310467-v2wlu5eq 36 9 min min NN cord-310467-v2wlu5eq 36 10 at at IN cord-310467-v2wlu5eq 36 11 RT RT NNP cord-310467-v2wlu5eq 36 12 . . . cord-310467-v2wlu5eq 36 13 _SP cord-310467-v2wlu5eq 37 1 An an DT cord-310467-v2wlu5eq 37 2 IiT iit NN cord-310467-v2wlu5eq 37 3 previously previously RB cord-310467-v2wlu5eq 37 4 developed develop VBD cord-310467-v2wlu5eq 37 5 for for IN cord-310467-v2wlu5eq 37 6 the the DT cord-310467-v2wlu5eq 37 7 detection detection NN cord-310467-v2wlu5eq 37 8 of of IN cord-310467-v2wlu5eq 37 9 surface surface NN cord-310467-v2wlu5eq 37 10 viral viral JJ cord-310467-v2wlu5eq 37 11 antigens antigen NNS cord-310467-v2wlu5eq 37 12 induced induce VBN cord-310467-v2wlu5eq 37 13 by by IN cord-310467-v2wlu5eq 37 14 the the DT cord-310467-v2wlu5eq 37 15 Purdue-115 Purdue-115 NNPS cord-310467-v2wlu5eq 37 16 strain strain NN cord-310467-v2wlu5eq 37 17 in in IN cord-310467-v2wlu5eq 37 18 infected infected JJ cord-310467-v2wlu5eq 37 19 ST ST NNP cord-310467-v2wlu5eq 37 20 cells cell NNS cord-310467-v2wlu5eq 37 21 ( ( -LRB- cord-310467-v2wlu5eq 37 22 T6 T6 NNP cord-310467-v2wlu5eq 37 23 et et NNP cord-310467-v2wlu5eq 37 24 al al NNP cord-310467-v2wlu5eq 37 25 . . NNP cord-310467-v2wlu5eq 37 26 , , , cord-310467-v2wlu5eq 37 27 1991 1991 CD cord-310467-v2wlu5eq 37 28 ) ) -RRB- cord-310467-v2wlu5eq 37 29 was be VBD cord-310467-v2wlu5eq 37 30 used use VBN cord-310467-v2wlu5eq 37 31 . . . cord-310467-v2wlu5eq 38 1 Briefly briefly RB cord-310467-v2wlu5eq 38 2 , , , cord-310467-v2wlu5eq 38 3 the the DT cord-310467-v2wlu5eq 38 4 fixed fix VBN cord-310467-v2wlu5eq 38 5 cells cell NNS cord-310467-v2wlu5eq 38 6 were be VBD cord-310467-v2wlu5eq 38 7 washed wash VBN cord-310467-v2wlu5eq 38 8 gently gently RB cord-310467-v2wlu5eq 38 9 with with IN cord-310467-v2wlu5eq 38 10 PBS PBS NNP cord-310467-v2wlu5eq 38 11 and and CC cord-310467-v2wlu5eq 38 12 were be VBD cord-310467-v2wlu5eq 38 13 incubated incubate VBN cord-310467-v2wlu5eq 38 14 for for IN cord-310467-v2wlu5eq 38 15 90 90 CD cord-310467-v2wlu5eq 38 16 min min NN cord-310467-v2wlu5eq 38 17 at at IN cord-310467-v2wlu5eq 38 18 4 4 CD cord-310467-v2wlu5eq 38 19 ° ° NN cord-310467-v2wlu5eq 38 20 C c NN cord-310467-v2wlu5eq 38 21 with with IN cord-310467-v2wlu5eq 38 22 0.1 0.1 CD cord-310467-v2wlu5eq 38 23 ml/ ml/ XX cord-310467-v2wlu5eq 39 1 well well UH cord-310467-v2wlu5eq 39 2 of of IN cord-310467-v2wlu5eq 39 3 a a DT cord-310467-v2wlu5eq 39 4 predetermined predetermine VBN cord-310467-v2wlu5eq 39 5 dilution dilution NN cord-310467-v2wlu5eq 39 6 of of IN cord-310467-v2wlu5eq 39 7 anti anti JJ cord-310467-v2wlu5eq 39 8 - - NNP cord-310467-v2wlu5eq 39 9 M m JJ cord-310467-v2wlu5eq 39 10 , , , cord-310467-v2wlu5eq 39 11 anti anti NNP cord-310467-v2wlu5eq 39 12 - - NNP cord-310467-v2wlu5eq 39 13 S s JJ cord-310467-v2wlu5eq 39 14 , , , cord-310467-v2wlu5eq 39 15 anti anti JJ cord-310467-v2wlu5eq 39 16 - - JJ cord-310467-v2wlu5eq 39 17 N n JJ cord-310467-v2wlu5eq 39 18 MAb MAb NNS cord-310467-v2wlu5eq 39 19 or or CC cord-310467-v2wlu5eq 39 20 anti anti JJ cord-310467-v2wlu5eq 39 21 - - JJ cord-310467-v2wlu5eq 39 22 TGEV tgev JJ cord-310467-v2wlu5eq 39 23 PAbs PAbs NNP cord-310467-v2wlu5eq 39 24 . . . cord-310467-v2wlu5eq 40 1 The the DT cord-310467-v2wlu5eq 40 2 reagents reagent NNS cord-310467-v2wlu5eq 40 3 were be VBD cord-310467-v2wlu5eq 40 4 removed remove VBN cord-310467-v2wlu5eq 40 5 from from IN cord-310467-v2wlu5eq 40 6 the the DT cord-310467-v2wlu5eq 40 7 plates plate NNS cord-310467-v2wlu5eq 40 8 by by IN cord-310467-v2wlu5eq 40 9 two two CD cord-310467-v2wlu5eq 40 10 rinses rinse NNS cord-310467-v2wlu5eq 40 11 with with IN cord-310467-v2wlu5eq 40 12 tap tap NN cord-310467-v2wlu5eq 40 13 - - HYPH cord-310467-v2wlu5eq 40 14 water water NN cord-310467-v2wlu5eq 40 15 and and CC cord-310467-v2wlu5eq 40 16 two two CD cord-310467-v2wlu5eq 40 17 washes wash NNS cord-310467-v2wlu5eq 40 18 with with IN cord-310467-v2wlu5eq 40 19 PBS PBS NNP cord-310467-v2wlu5eq 40 20 containing contain VBG cord-310467-v2wlu5eq 40 21 0.05 0.05 CD cord-310467-v2wlu5eq 40 22 % % NN cord-310467-v2wlu5eq 40 23 Tween Tween NNP cord-310467-v2wlu5eq 40 24 20 20 CD cord-310467-v2wlu5eq 40 25 ( ( -LRB- cord-310467-v2wlu5eq 40 26 Serva serva NN cord-310467-v2wlu5eq 40 27 ) ) -RRB- cord-310467-v2wlu5eq 40 28 and and CC cord-310467-v2wlu5eq 40 29 then then RB cord-310467-v2wlu5eq 40 30 replaced replace VBN cord-310467-v2wlu5eq 40 31 by by IN cord-310467-v2wlu5eq 40 32 0.1 0.1 CD cord-310467-v2wlu5eq 40 33 ml/ ml/ XX cord-310467-v2wlu5eq 41 1 well well UH cord-310467-v2wlu5eq 41 2 of of IN cord-310467-v2wlu5eq 41 3 a a DT cord-310467-v2wlu5eq 41 4 working work VBG cord-310467-v2wlu5eq 41 5 dilution dilution NN cord-310467-v2wlu5eq 41 6 of of IN cord-310467-v2wlu5eq 41 7 peroxidase peroxidase NN cord-310467-v2wlu5eq 41 8 - - HYPH cord-310467-v2wlu5eq 41 9 labelled label VBN cord-310467-v2wlu5eq 41 10 goat goat NN cord-310467-v2wlu5eq 41 11 anti anti JJ cord-310467-v2wlu5eq 41 12 - - JJ cord-310467-v2wlu5eq 41 13 mouse mouse NN cord-310467-v2wlu5eq 41 14 Fc Fc NNP cord-310467-v2wlu5eq 41 15 serum serum NN cord-310467-v2wlu5eq 41 16 ( ( -LRB- cord-310467-v2wlu5eq 41 17 ICN ICN NNP cord-310467-v2wlu5eq 41 18 ImmunoBiologicals ImmunoBiologicals NNP cord-310467-v2wlu5eq 41 19 , , , cord-310467-v2wlu5eq 41 20 Israel Israel NNP cord-310467-v2wlu5eq 41 21 ) ) -RRB- cord-310467-v2wlu5eq 41 22 or or CC cord-310467-v2wlu5eq 41 23 peroxidaseu peroxidaseu VBZ cord-310467-v2wlu5eq 41 24 197 197 CD cord-310467-v2wlu5eq 41 25 labelled label VBN cord-310467-v2wlu5eq 41 26 rabbit rabbit NN cord-310467-v2wlu5eq 41 27 anti anti JJ cord-310467-v2wlu5eq 41 28 - - JJ cord-310467-v2wlu5eq 41 29 swine swine JJ cord-310467-v2wlu5eq 41 30 ( ( -LRB- cord-310467-v2wlu5eq 41 31 heavy heavy JJ cord-310467-v2wlu5eq 41 32 and and CC cord-310467-v2wlu5eq 41 33 light light JJ cord-310467-v2wlu5eq 41 34 chain chain NN cord-310467-v2wlu5eq 41 35 specific specific JJ cord-310467-v2wlu5eq 41 36 ) ) -RRB- cord-310467-v2wlu5eq 41 37 serum serum NN cord-310467-v2wlu5eq 41 38 ( ( -LRB- cord-310467-v2wlu5eq 41 39 Cappei Cappei NNP cord-310467-v2wlu5eq 41 40 , , , cord-310467-v2wlu5eq 41 41 Organon Organon NNP cord-310467-v2wlu5eq 41 42 Teknica Teknica NNP cord-310467-v2wlu5eq 41 43 , , , cord-310467-v2wlu5eq 41 44 France France NNP cord-310467-v2wlu5eq 41 45 ) ) -RRB- cord-310467-v2wlu5eq 41 46 . . . cord-310467-v2wlu5eq 42 1 After after IN cord-310467-v2wlu5eq 42 2 incubation incubation NN cord-310467-v2wlu5eq 42 3 at at IN cord-310467-v2wlu5eq 42 4 4 4 CD cord-310467-v2wlu5eq 42 5 ° ° NN cord-310467-v2wlu5eq 42 6 C c NN cord-310467-v2wlu5eq 42 7 for for IN cord-310467-v2wlu5eq 42 8 90 90 CD cord-310467-v2wlu5eq 42 9 min min NN cord-310467-v2wlu5eq 42 10 , , , cord-310467-v2wlu5eq 42 11 the the DT cord-310467-v2wlu5eq 42 12 plates plate NNS cord-310467-v2wlu5eq 42 13 were be VBD cord-310467-v2wlu5eq 42 14 washed wash VBN cord-310467-v2wlu5eq 42 15 as as IN cord-310467-v2wlu5eq 42 16 before before RB cord-310467-v2wlu5eq 42 17 and and CC cord-310467-v2wlu5eq 42 18 the the DT cord-310467-v2wlu5eq 42 19 enzymatic enzymatic JJ cord-310467-v2wlu5eq 42 20 reaction reaction NN cord-310467-v2wlu5eq 42 21 was be VBD cord-310467-v2wlu5eq 42 22 developed develop VBN cord-310467-v2wlu5eq 42 23 by by IN cord-310467-v2wlu5eq 42 24 incubation incubation NN cord-310467-v2wlu5eq 42 25 at at IN cord-310467-v2wlu5eq 42 26 37'C 37'c CD cord-310467-v2wlu5eq 42 27 for for IN cord-310467-v2wlu5eq 42 28 1 1 CD cord-310467-v2wlu5eq 42 29 h h NN cord-310467-v2wlu5eq 42 30 with with IN cord-310467-v2wlu5eq 42 31 2,2'-azino 2,2'-azino CD cord-310467-v2wlu5eq 42 32 - - HYPH cord-310467-v2wlu5eq 42 33 bis bis NN cord-310467-v2wlu5eq 42 34 ( ( -LRB- cord-310467-v2wlu5eq 42 35 3-ethyl 3-ethyl CD cord-310467-v2wlu5eq 42 36 benzthiazoline-6 benzthiazoline-6 NNP cord-310467-v2wlu5eq 42 37 sulphonic sulphonic NNP cord-310467-v2wlu5eq 42 38 acid acid NN cord-310467-v2wlu5eq 42 39 ) ) -RRB- cord-310467-v2wlu5eq 43 1 ( ( -LRB- cord-310467-v2wlu5eq 43 2 ABTS ABTS NNP cord-310467-v2wlu5eq 43 3 ( ( -LRB- cord-310467-v2wlu5eq 43 4 Boehringer Boehringer NNP cord-310467-v2wlu5eq 43 5 , , , cord-310467-v2wlu5eq 43 6 Mannheim Mannheim NNP cord-310467-v2wlu5eq 43 7 , , , cord-310467-v2wlu5eq 43 8 Germany))/H20 germany))/h20 NN cord-310467-v2wlu5eq 43 9 2 2 CD cord-310467-v2wlu5eq 43 10 chromogen chromogen NN cord-310467-v2wlu5eq 43 11 / / SYM cord-310467-v2wlu5eq 43 12 substrate substrate NN cord-310467-v2wlu5eq 43 13 solution solution NN cord-310467-v2wlu5eq 43 14 . . . cord-310467-v2wlu5eq 44 1 The the DT cord-310467-v2wlu5eq 44 2 supernatants supernatant NNS cord-310467-v2wlu5eq 44 3 were be VBD cord-310467-v2wlu5eq 44 4 transferred transfer VBN cord-310467-v2wlu5eq 44 5 to to IN cord-310467-v2wlu5eq 44 6 another another DT cord-310467-v2wlu5eq 44 7 plate plate NN cord-310467-v2wlu5eq 44 8 containing contain VBG cord-310467-v2wlu5eq 44 9 0.02 0.02 CD cord-310467-v2wlu5eq 44 10 ml ml NNS cord-310467-v2wlu5eq 44 11 of of IN cord-310467-v2wlu5eq 44 12 10 10 CD cord-310467-v2wlu5eq 44 13 % % NN cord-310467-v2wlu5eq 44 14 sodium sodium NN cord-310467-v2wlu5eq 44 15 dodecyl dodecyl NNP cord-310467-v2wlu5eq 44 16 sulphate sulphate NNP cord-310467-v2wlu5eq 44 17 ( ( -LRB- cord-310467-v2wlu5eq 44 18 SDS SDS NNP cord-310467-v2wlu5eq 44 19 ) ) -RRB- cord-310467-v2wlu5eq 44 20 ( ( -LRB- cord-310467-v2wlu5eq 44 21 Serva serva NN cord-310467-v2wlu5eq 44 22 ) ) -RRB- cord-310467-v2wlu5eq 44 23 to to TO cord-310467-v2wlu5eq 44 24 stop stop VB cord-310467-v2wlu5eq 44 25 the the DT cord-310467-v2wlu5eq 44 26 enzymic enzymic NNP cord-310467-v2wlu5eq 44 27 reaction reaction NN cord-310467-v2wlu5eq 44 28 and and CC cord-310467-v2wlu5eq 44 29 to to TO cord-310467-v2wlu5eq 44 30 permit permit VB cord-310467-v2wlu5eq 44 31 the the DT cord-310467-v2wlu5eq 44 32 reading reading NN cord-310467-v2wlu5eq 44 33 of of IN cord-310467-v2wlu5eq 44 34 the the DT cord-310467-v2wlu5eq 44 35 plate plate NN cord-310467-v2wlu5eq 44 36 . . . cord-310467-v2wlu5eq 45 1 The the DT cord-310467-v2wlu5eq 45 2 optical optical JJ cord-310467-v2wlu5eq 45 3 density density NN cord-310467-v2wlu5eq 45 4 ( ( -LRB- cord-310467-v2wlu5eq 45 5 OD OD NNP cord-310467-v2wlu5eq 45 6 ) ) -RRB- cord-310467-v2wlu5eq 45 7 was be VBD cord-310467-v2wlu5eq 45 8 measured measure VBN cord-310467-v2wlu5eq 45 9 at at IN cord-310467-v2wlu5eq 45 10 415 415 CD cord-310467-v2wlu5eq 45 11 nm nm NN cord-310467-v2wlu5eq 45 12 by by IN cord-310467-v2wlu5eq 45 13 the the DT cord-310467-v2wlu5eq 45 14 ELISA ELISA NNP cord-310467-v2wlu5eq 45 15 reader reader NN cord-310467-v2wlu5eq 45 16 ( ( -LRB- cord-310467-v2wlu5eq 45 17 Titertek Titertek NNP cord-310467-v2wlu5eq 45 18 Multiskan Multiskan NNP cord-310467-v2wlu5eq 45 19 ) ) -RRB- cord-310467-v2wlu5eq 45 20 coupled couple VBD cord-310467-v2wlu5eq 45 21 to to IN cord-310467-v2wlu5eq 45 22 a a DT cord-310467-v2wlu5eq 45 23 microcomputer microcomputer NN cord-310467-v2wlu5eq 45 24 for for IN cord-310467-v2wlu5eq 45 25 data datum NNS cord-310467-v2wlu5eq 45 26 storage storage NN cord-310467-v2wlu5eq 45 27 and and CC cord-310467-v2wlu5eq 45 28 statistical statistical JJ cord-310467-v2wlu5eq 45 29 processing processing NN cord-310467-v2wlu5eq 45 30 . . . cord-310467-v2wlu5eq 46 1 The the DT cord-310467-v2wlu5eq 46 2 OD OD NNP cord-310467-v2wlu5eq 46 3 signals signal NNS cord-310467-v2wlu5eq 46 4 obtained obtain VBN cord-310467-v2wlu5eq 46 5 from from IN cord-310467-v2wlu5eq 46 6 the the DT cord-310467-v2wlu5eq 46 7 liT lit NN cord-310467-v2wlu5eq 46 8 are be VBP cord-310467-v2wlu5eq 46 9 composed compose VBN cord-310467-v2wlu5eq 46 10 of of IN cord-310467-v2wlu5eq 46 11 three three CD cord-310467-v2wlu5eq 46 12 components component NNS cord-310467-v2wlu5eq 46 13 : : : cord-310467-v2wlu5eq 46 14 ( ( -LRB- cord-310467-v2wlu5eq 46 15 a a LS cord-310467-v2wlu5eq 46 16 ) ) -RRB- cord-310467-v2wlu5eq 46 17 a a DT cord-310467-v2wlu5eq 46 18 specific specific JJ cord-310467-v2wlu5eq 46 19 OD od NN cord-310467-v2wlu5eq 46 20 signal signal NN cord-310467-v2wlu5eq 46 21 generated generate VBN cord-310467-v2wlu5eq 46 22 by by IN cord-310467-v2wlu5eq 46 23 the the DT cord-310467-v2wlu5eq 46 24 second second JJ cord-310467-v2wlu5eq 46 25 - - HYPH cord-310467-v2wlu5eq 46 26 step step NN cord-310467-v2wlu5eq 46 27 conjugate conjugate NN cord-310467-v2wlu5eq 46 28 specifically specifically RB cord-310467-v2wlu5eq 46 29 bound bind VBN cord-310467-v2wlu5eq 46 30 to to IN cord-310467-v2wlu5eq 46 31 the the DT cord-310467-v2wlu5eq 46 32 antigen antigen NN cord-310467-v2wlu5eq 46 33 - - HYPH cord-310467-v2wlu5eq 46 34 bound bind VBN cord-310467-v2wlu5eq 46 35 mona mona NNP cord-310467-v2wlu5eq 46 36 clonal clonal JJ cord-310467-v2wlu5eq 46 37 antibody antibody NN cord-310467-v2wlu5eq 46 38 ; ; : cord-310467-v2wlu5eq 46 39 ( ( -LRB- cord-310467-v2wlu5eq 46 40 b b LS cord-310467-v2wlu5eq 46 41 ) ) -RRB- cord-310467-v2wlu5eq 46 42 non non JJ cord-310467-v2wlu5eq 46 43 - - JJ cord-310467-v2wlu5eq 46 44 specific specific JJ cord-310467-v2wlu5eq 46 45 OD OD NNP cord-310467-v2wlu5eq 46 46 generated generate VBN cord-310467-v2wlu5eq 46 47 by by IN cord-310467-v2wlu5eq 46 48 non non AFX cord-310467-v2wlu5eq 46 49 - - JJ cord-310467-v2wlu5eq 46 50 specifically specifically RB cord-310467-v2wlu5eq 46 51 bound bind VBN cord-310467-v2wlu5eq 46 52 second second JJ cord-310467-v2wlu5eq 46 53 - - HYPH cord-310467-v2wlu5eq 46 54 step step NN cord-310467-v2wlu5eq 46 55 conjugate conjugate NN cord-310467-v2wlu5eq 46 56 ; ; , cord-310467-v2wlu5eq 46 57 and and CC cord-310467-v2wlu5eq 46 58 ( ( -LRB- cord-310467-v2wlu5eq 46 59 c c NN cord-310467-v2wlu5eq 46 60 ) ) -RRB- cord-310467-v2wlu5eq 46 61 nonspecific nonspecific JJ cord-310467-v2wlu5eq 46 62 spontaneous spontaneous JJ cord-310467-v2wlu5eq 46 63 OD od NN cord-310467-v2wlu5eq 46 64 due due IN cord-310467-v2wlu5eq 46 65 to to IN cord-310467-v2wlu5eq 46 66 other other JJ cord-310467-v2wlu5eq 46 67 reasons reason NNS cord-310467-v2wlu5eq 46 68 , , , cord-310467-v2wlu5eq 46 69 e.g. e.g. RB cord-310467-v2wlu5eq 46 70 , , , cord-310467-v2wlu5eq 46 71 spontaneous spontaneous JJ cord-310467-v2wlu5eq 46 72 decay decay NN cord-310467-v2wlu5eq 46 73 of of IN cord-310467-v2wlu5eq 46 74 the the DT cord-310467-v2wlu5eq 46 75 substrate substrate NN cord-310467-v2wlu5eq 46 76 or or CC cord-310467-v2wlu5eq 46 77 the the DT cord-310467-v2wlu5eq 46 78 intrinsic intrinsic JJ cord-310467-v2wlu5eq 46 79 OD od NN cord-310467-v2wlu5eq 46 80 of of IN cord-310467-v2wlu5eq 46 81 the the DT cord-310467-v2wlu5eq 46 82 cells cell NNS cord-310467-v2wlu5eq 46 83 . . . cord-310467-v2wlu5eq 47 1 The the DT cord-310467-v2wlu5eq 47 2 first first JJ cord-310467-v2wlu5eq 47 3 component component NN cord-310467-v2wlu5eq 47 4 is be VBZ cord-310467-v2wlu5eq 47 5 the the DT cord-310467-v2wlu5eq 47 6 one one CD cord-310467-v2wlu5eq 47 7 of of IN cord-310467-v2wlu5eq 47 8 interest interest NN cord-310467-v2wlu5eq 47 9 . . . cord-310467-v2wlu5eq 48 1 The the DT cord-310467-v2wlu5eq 48 2 other other JJ cord-310467-v2wlu5eq 48 3 two two CD cord-310467-v2wlu5eq 48 4 contribute contribute VBP cord-310467-v2wlu5eq 48 5 non non NN cord-310467-v2wlu5eq 48 6 - - NN cord-310467-v2wlu5eq 48 7 specifically specifically RB cord-310467-v2wlu5eq 48 8 to to IN cord-310467-v2wlu5eq 48 9 the the DT cord-310467-v2wlu5eq 48 10 OD OD NNP cord-310467-v2wlu5eq 48 11 signal signal NN cord-310467-v2wlu5eq 48 12 and and CC cord-310467-v2wlu5eq 48 13 therefore therefore RB cord-310467-v2wlu5eq 48 14 should should MD cord-310467-v2wlu5eq 48 15 be be VB cord-310467-v2wlu5eq 48 16 corrected correct VBN cord-310467-v2wlu5eq 48 17 in in IN cord-310467-v2wlu5eq 48 18 order order NN cord-310467-v2wlu5eq 48 19 to to TO cord-310467-v2wlu5eq 48 20 determine determine VB cord-310467-v2wlu5eq 48 21 the the DT cord-310467-v2wlu5eq 48 22 specific specific JJ cord-310467-v2wlu5eq 48 23 signal signal NN cord-310467-v2wlu5eq 48 24 . . . cord-310467-v2wlu5eq 49 1 The the DT cord-310467-v2wlu5eq 49 2 quantity quantity NN cord-310467-v2wlu5eq 49 3 of of IN cord-310467-v2wlu5eq 49 4 each each DT cord-310467-v2wlu5eq 49 5 antigen antigen NN cord-310467-v2wlu5eq 49 6 , , , cord-310467-v2wlu5eq 49 7 tested test VBN cord-310467-v2wlu5eq 49 8 in in IN cord-310467-v2wlu5eq 49 9 quadruplicate quadruplicate NNP cord-310467-v2wlu5eq 49 10 , , , cord-310467-v2wlu5eq 49 11 was be VBD cord-310467-v2wlu5eq 49 12 expressed express VBN cord-310467-v2wlu5eq 49 13 as as IN cord-310467-v2wlu5eq 49 14 the the DT cord-310467-v2wlu5eq 49 15 difference difference NN cord-310467-v2wlu5eq 49 16 between between IN cord-310467-v2wlu5eq 49 17 the the DT cord-310467-v2wlu5eq 49 18 OD OD NNP cord-310467-v2wlu5eq 49 19 at at IN cord-310467-v2wlu5eq 49 20 415 415 CD cord-310467-v2wlu5eq 49 21 nm nm NN cord-310467-v2wlu5eq 49 22 of of IN cord-310467-v2wlu5eq 49 23 virus virus NN cord-310467-v2wlu5eq 49 24 - - HYPH cord-310467-v2wlu5eq 49 25 infected infect VBN cord-310467-v2wlu5eq 49 26 and and CC cord-310467-v2wlu5eq 49 27 uninfected uninfected JJ cord-310467-v2wlu5eq 49 28 cells cell NNS cord-310467-v2wlu5eq 49 29 using use VBG cord-310467-v2wlu5eq 49 30 the the DT cord-310467-v2wlu5eq 49 31 formula formula NN cord-310467-v2wlu5eq 49 32 : : : cord-310467-v2wlu5eq 49 33 For for IN cord-310467-v2wlu5eq 49 34 comparative comparative JJ cord-310467-v2wlu5eq 49 35 purposes purpose NNS cord-310467-v2wlu5eq 49 36 the the DT cord-310467-v2wlu5eq 49 37 degree degree NN cord-310467-v2wlu5eq 49 38 of of IN cord-310467-v2wlu5eq 49 39 relative relative JJ cord-310467-v2wlu5eq 49 40 antibody antibody NN cord-310467-v2wlu5eq 49 41 binding bind VBG cord-310467-v2wlu5eq 49 42 to to IN cord-310467-v2wlu5eq 49 43 a a DT cord-310467-v2wlu5eq 49 44 specified specify VBN cord-310467-v2wlu5eq 49 45 determinant determinant NN cord-310467-v2wlu5eq 49 46 was be VBD cord-310467-v2wlu5eq 49 47 expressed express VBN cord-310467-v2wlu5eq 49 48 as as IN cord-310467-v2wlu5eq 49 49 a a DT cord-310467-v2wlu5eq 49 50 percentage percentage NN cord-310467-v2wlu5eq 49 51 of of IN cord-310467-v2wlu5eq 49 52 antibody antibody NN cord-310467-v2wlu5eq 49 53 binding bind VBG cord-310467-v2wlu5eq 49 54 to to IN cord-310467-v2wlu5eq 49 55 unfixed unfixed JJ cord-310467-v2wlu5eq 49 56 infected infected JJ cord-310467-v2wlu5eq 49 57 cells cell NNS cord-310467-v2wlu5eq 49 58 . . . cord-310467-v2wlu5eq 50 1 The the DT cord-310467-v2wlu5eq 50 2 effects effect NNS cord-310467-v2wlu5eq 50 3 of of IN cord-310467-v2wlu5eq 50 4 cell cell NN cord-310467-v2wlu5eq 50 5 fixation fixation NN cord-310467-v2wlu5eq 50 6 with with IN cord-310467-v2wlu5eq 50 7 FA FA NNP cord-310467-v2wlu5eq 50 8 , , , cord-310467-v2wlu5eq 50 9 GA GA NNP cord-310467-v2wlu5eq 50 10 and and CC cord-310467-v2wlu5eq 50 11 PFA PFA NNP cord-310467-v2wlu5eq 50 12 at at IN cord-310467-v2wlu5eq 50 13 various various JJ cord-310467-v2wlu5eq 50 14 concentrations concentration NNS cord-310467-v2wlu5eq 50 15 on on IN cord-310467-v2wlu5eq 50 16 antibody antibody NN cord-310467-v2wlu5eq 50 17 bind bind NN cord-310467-v2wlu5eq 50 18 - - HYPH cord-310467-v2wlu5eq 50 19 ing e VBG cord-310467-v2wlu5eq 50 20 to to IN cord-310467-v2wlu5eq 50 21 viral viral JJ cord-310467-v2wlu5eq 50 22 determinants determinant NNS cord-310467-v2wlu5eq 50 23 in in IN cord-310467-v2wlu5eq 50 24 the the DT cord-310467-v2wlu5eq 50 25 TGEV TGEV NNP cord-310467-v2wlu5eq 50 26 - - HYPH cord-310467-v2wlu5eq 50 27 infected infect VBN cord-310467-v2wlu5eq 50 28 cells cell NNS cord-310467-v2wlu5eq 50 29 are be VBP cord-310467-v2wlu5eq 50 30 shown show VBN cord-310467-v2wlu5eq 50 31 in in IN cord-310467-v2wlu5eq 50 32 Fig Fig NNP cord-310467-v2wlu5eq 50 33 . . . cord-310467-v2wlu5eq 51 1 1 1 LS cord-310467-v2wlu5eq 51 2 . . . cord-310467-v2wlu5eq 52 1 Antibody antibody NN cord-310467-v2wlu5eq 52 2 binding bind VBG cord-310467-v2wlu5eq 52 3 to to IN cord-310467-v2wlu5eq 52 4 the the DT cord-310467-v2wlu5eq 52 5 N n CD cord-310467-v2wlu5eq 52 6 determinant determinant NN cord-310467-v2wlu5eq 52 7 was be VBD cord-310467-v2wlu5eq 52 8 close close JJ cord-310467-v2wlu5eq 52 9 to to IN cord-310467-v2wlu5eq 52 10 zero zero CD cord-310467-v2wlu5eq 52 11 when when WRB cord-310467-v2wlu5eq 52 12 cells cell NNS cord-310467-v2wlu5eq 52 13 were be VBD cord-310467-v2wlu5eq 52 14 fixed fix VBN cord-310467-v2wlu5eq 52 15 with with IN cord-310467-v2wlu5eq 52 16 all all PDT cord-310467-v2wlu5eq 52 17 these these DT cord-310467-v2wlu5eq 52 18 three three CD cord-310467-v2wlu5eq 52 19 aldehyde aldehyde NN cord-310467-v2wlu5eq 52 20 fixatives fixative NNS cord-310467-v2wlu5eq 52 21 . . . cord-310467-v2wlu5eq 53 1 Fixation fixation NN cord-310467-v2wlu5eq 53 2 with with IN cord-310467-v2wlu5eq 53 3 FA FA NNP cord-310467-v2wlu5eq 53 4 at at IN cord-310467-v2wlu5eq 53 5 different different JJ cord-310467-v2wlu5eq 53 6 concentrations concentration NNS cord-310467-v2wlu5eq 53 7 led lead VBD cord-310467-v2wlu5eq 53 8 to to IN cord-310467-v2wlu5eq 53 9 irregular irregular JJ cord-310467-v2wlu5eq 53 10 patterns pattern NNS cord-310467-v2wlu5eq 53 11 for for IN cord-310467-v2wlu5eq 53 12 antibody antibody NN cord-310467-v2wlu5eq 53 13 binding bind VBG cord-310467-v2wlu5eq 53 14 to to IN cord-310467-v2wlu5eq 53 15 M M NNP cord-310467-v2wlu5eq 53 16 and and CC cord-310467-v2wlu5eq 53 17 S S NNP cord-310467-v2wlu5eq 53 18 determinants determinant NNS cord-310467-v2wlu5eq 54 1 ( ( -LRB- cord-310467-v2wlu5eq 54 2 Fig Fig NNP cord-310467-v2wlu5eq 54 3 . . . cord-310467-v2wlu5eq 54 4 la la NNP cord-310467-v2wlu5eq 54 5 ) ) -RRB- cord-310467-v2wlu5eq 54 6 . . . cord-310467-v2wlu5eq 55 1 FA FA NNP cord-310467-v2wlu5eq 55 2 at at IN cord-310467-v2wlu5eq 55 3 high high JJ cord-310467-v2wlu5eq 55 4 concentrations concentration NNS cord-310467-v2wlu5eq 55 5 caused cause VBD cord-310467-v2wlu5eq 55 6 a a DT cord-310467-v2wlu5eq 55 7 decrease decrease NN cord-310467-v2wlu5eq 55 8 of of IN cord-310467-v2wlu5eq 55 9 antibody antibody NN cord-310467-v2wlu5eq 55 10 binding bind VBG cord-310467-v2wlu5eq 55 11 to to IN cord-310467-v2wlu5eq 55 12 S s NN cord-310467-v2wlu5eq 55 13 determinant determinant NN cord-310467-v2wlu5eq 55 14 but but CC cord-310467-v2wlu5eq 55 15 an an DT cord-310467-v2wlu5eq 55 16 increase increase NN cord-310467-v2wlu5eq 55 17 of of IN cord-310467-v2wlu5eq 55 18 the the DT cord-310467-v2wlu5eq 55 19 M M NNP cord-310467-v2wlu5eq 55 20 determinant determinant NN cord-310467-v2wlu5eq 55 21 up up IN cord-310467-v2wlu5eq 55 22 to to TO cord-310467-v2wlu5eq 55 23 175 175 CD cord-310467-v2wlu5eq 55 24 % % NN cord-310467-v2wlu5eq 55 25 of of IN cord-310467-v2wlu5eq 55 26 controls control NNS cord-310467-v2wlu5eq 55 27 at at IN cord-310467-v2wlu5eq 55 28 a a DT cord-310467-v2wlu5eq 55 29 FA FA NNP cord-310467-v2wlu5eq 55 30 concentration concentration NN cord-310467-v2wlu5eq 55 31 of of IN cord-310467-v2wlu5eq 55 32 0.5 0.5 CD cord-310467-v2wlu5eq 55 33 % % NN cord-310467-v2wlu5eq 55 34 . . . cord-310467-v2wlu5eq 56 1 The the DT cord-310467-v2wlu5eq 56 2 levels level NNS cord-310467-v2wlu5eq 56 3 of of IN cord-310467-v2wlu5eq 56 4 antibody antibody NN cord-310467-v2wlu5eq 56 5 binding bind VBG cord-310467-v2wlu5eq 56 6 to to IN cord-310467-v2wlu5eq 56 7 M M NNP cord-310467-v2wlu5eq 56 8 and and CC cord-310467-v2wlu5eq 56 9 S S NNP cord-310467-v2wlu5eq 56 10 determinants determinant NNS cord-310467-v2wlu5eq 56 11 were be VBD cord-310467-v2wlu5eq 56 12 very very RB cord-310467-v2wlu5eq 56 13 low low JJ cord-310467-v2wlu5eq 56 14 using use VBG cord-310467-v2wlu5eq 56 15 fixation fixation NN cord-310467-v2wlu5eq 56 16 with with IN cord-310467-v2wlu5eq 56 17 GA GA NNP cord-310467-v2wlu5eq 56 18 at at IN cord-310467-v2wlu5eq 56 19 different different JJ cord-310467-v2wlu5eq 56 20 concentrations concentration NNS cord-310467-v2wlu5eq 56 21 ( ( -LRB- cord-310467-v2wlu5eq 56 22 Fig Fig NNP cord-310467-v2wlu5eq 56 23 . . . cord-310467-v2wlu5eq 56 24 lb lb CD cord-310467-v2wlu5eq 56 25 ) ) -RRB- cord-310467-v2wlu5eq 56 26 . . . cord-310467-v2wlu5eq 57 1 and and CC cord-310467-v2wlu5eq 57 2 $ $ $ cord-310467-v2wlu5eq 57 3 ( ( -LRB- cord-310467-v2wlu5eq 57 4 51 51 CD cord-310467-v2wlu5eq 57 5 - - SYM cord-310467-v2wlu5eq 57 6 13 13 CD cord-310467-v2wlu5eq 57 7 ) ) -RRB- cord-310467-v2wlu5eq 57 8 determinants determinant NNS cord-310467-v2wlu5eq 57 9 were be VBD cord-310467-v2wlu5eq 57 10 expressed express VBN cord-310467-v2wlu5eq 57 11 as as IN cord-310467-v2wlu5eq 57 12 percentages percentage NNS cord-310467-v2wlu5eq 57 13 compared compare VBN cord-310467-v2wlu5eq 57 14 with with IN cord-310467-v2wlu5eq 57 15 the the DT cord-310467-v2wlu5eq 57 16 value value NN cord-310467-v2wlu5eq 57 17 obtained obtain VBN cord-310467-v2wlu5eq 57 18 with with IN cord-310467-v2wlu5eq 57 19 0.1 0.1 CD cord-310467-v2wlu5eq 57 20 % % NN cord-310467-v2wlu5eq 57 21 paraformaldehyde paraformaldehyde NN cord-310467-v2wlu5eq 57 22 - - HYPH cord-310467-v2wlu5eq 57 23 fixed fix VBN cord-310467-v2wlu5eq 57 24 , , , cord-310467-v2wlu5eq 57 25 TGEV TGEV NNP cord-310467-v2wlu5eq 57 26 - - HYPH cord-310467-v2wlu5eq 57 27 infected infect VBN cord-310467-v2wlu5eq 57 28 cells cell NNS cord-310467-v2wlu5eq 57 29 . . . cord-310467-v2wlu5eq 58 1 The the DT cord-310467-v2wlu5eq 58 2 degree degree NN cord-310467-v2wlu5eq 58 3 of of IN cord-310467-v2wlu5eq 58 4 antibody antibody NN cord-310467-v2wlu5eq 58 5 binding bind VBG cord-310467-v2wlu5eq 58 6 to to IN cord-310467-v2wlu5eq 58 7 the the DT cord-310467-v2wlu5eq 58 8 N N NNP cord-310467-v2wlu5eq 58 9 ( ( -LRB- cord-310467-v2wlu5eq 58 10 22 22 CD cord-310467-v2wlu5eq 58 11 - - SYM cord-310467-v2wlu5eq 58 12 6 6 CD cord-310467-v2wlu5eq 58 13 ) ) -RRB- cord-310467-v2wlu5eq 58 14 determinant determinant NN cord-310467-v2wlu5eq 58 15 was be VBD cord-310467-v2wlu5eq 58 16 expressed express VBN cord-310467-v2wlu5eq 58 17 as as IN cord-310467-v2wlu5eq 58 18 a a DT cord-310467-v2wlu5eq 58 19 percentage percentage NN cord-310467-v2wlu5eq 58 20 compared compare VBN cord-310467-v2wlu5eq 58 21 with with IN cord-310467-v2wlu5eq 58 22 that that DT cord-310467-v2wlu5eq 58 23 obtained obtain VBN cord-310467-v2wlu5eq 58 24 with with IN cord-310467-v2wlu5eq 58 25 80 80 CD cord-310467-v2wlu5eq 58 26 % % NN cord-310467-v2wlu5eq 58 27 acetone acetone NN cord-310467-v2wlu5eq 58 28 - - HYPH cord-310467-v2wlu5eq 58 29 fixed fix VBN cord-310467-v2wlu5eq 58 30 , , , cord-310467-v2wlu5eq 58 31 TGEV TGEV NNP cord-310467-v2wlu5eq 58 32 - - HYPH cord-310467-v2wlu5eq 58 33 infected infect VBN cord-310467-v2wlu5eq 58 34 cells cell NNS cord-310467-v2wlu5eq 58 35 . . . cord-310467-v2wlu5eq 59 1 infected infected JJ cord-310467-v2wlu5eq 59 2 cells cell NNS cord-310467-v2wlu5eq 59 3 which which WDT cord-310467-v2wlu5eq 59 4 were be VBD cord-310467-v2wlu5eq 59 5 fixed fix VBN cord-310467-v2wlu5eq 59 6 with with IN cord-310467-v2wlu5eq 59 7 0.1 0.1 CD cord-310467-v2wlu5eq 59 8 % % NN cord-310467-v2wlu5eq 59 9 PFA PFA NNP cord-310467-v2wlu5eq 59 10 and and CC cord-310467-v2wlu5eq 59 11 antibody antibody NN cord-310467-v2wlu5eq 59 12 binding bind VBG cord-310467-v2wlu5eq 59 13 profiles profile NNS cord-310467-v2wlu5eq 59 14 for for IN cord-310467-v2wlu5eq 59 15 these these DT cord-310467-v2wlu5eq 59 16 two two CD cord-310467-v2wlu5eq 59 17 determinants determinant NNS cord-310467-v2wlu5eq 59 18 agreed agree VBD cord-310467-v2wlu5eq 59 19 well well RB cord-310467-v2wlu5eq 60 1 ( ( -LRB- cord-310467-v2wlu5eq 60 2 Fig Fig NNP cord-310467-v2wlu5eq 60 3 . . . cord-310467-v2wlu5eq 60 4 lc lc NNP cord-310467-v2wlu5eq 60 5 ) ) -RRB- cord-310467-v2wlu5eq 60 6 . . . cord-310467-v2wlu5eq 61 1 It -PRON- PRP cord-310467-v2wlu5eq 61 2 is be VBZ cord-310467-v2wlu5eq 61 3 clear clear JJ cord-310467-v2wlu5eq 61 4 that that IN cord-310467-v2wlu5eq 61 5 , , , cord-310467-v2wlu5eq 61 6 among among IN cord-310467-v2wlu5eq 61 7 the the DT cord-310467-v2wlu5eq 61 8 aldehydes aldehyde NNS cord-310467-v2wlu5eq 61 9 tested test VBN cord-310467-v2wlu5eq 61 10 , , , cord-310467-v2wlu5eq 61 11 the the DT cord-310467-v2wlu5eq 61 12 M M NNP cord-310467-v2wlu5eq 61 13 and and CC cord-310467-v2wlu5eq 61 14 S S NNP cord-310467-v2wlu5eq 61 15 determinants determinant NNS cord-310467-v2wlu5eq 61 16 were be VBD cord-310467-v2wlu5eq 61 17 readily readily RB cord-310467-v2wlu5eq 61 18 detected detect VBN cord-310467-v2wlu5eq 61 19 in in IN cord-310467-v2wlu5eq 61 20 TGEV TGEV NNP cord-310467-v2wlu5eq 61 21 - - HYPH cord-310467-v2wlu5eq 61 22 infected infect VBN cord-310467-v2wlu5eq 61 23 cells cell NNS cord-310467-v2wlu5eq 61 24 which which WDT cord-310467-v2wlu5eq 61 25 were be VBD cord-310467-v2wlu5eq 61 26 fixed fix VBN cord-310467-v2wlu5eq 61 27 with with IN cord-310467-v2wlu5eq 61 28 0.1 0.1 CD cord-310467-v2wlu5eq 61 29 % % NN cord-310467-v2wlu5eq 61 30 PFA PFA NNP cord-310467-v2wlu5eq 61 31 . . . cord-310467-v2wlu5eq 62 1 The the DT cord-310467-v2wlu5eq 62 2 effects effect NNS cord-310467-v2wlu5eq 62 3 of of IN cord-310467-v2wlu5eq 62 4 cell cell NN cord-310467-v2wlu5eq 62 5 fixation fixation NN cord-310467-v2wlu5eq 62 6 on on IN cord-310467-v2wlu5eq 62 7 antibody antibody NN cord-310467-v2wlu5eq 62 8 binding bind VBG cord-310467-v2wlu5eq 62 9 to to IN cord-310467-v2wlu5eq 62 10 viral viral JJ cord-310467-v2wlu5eq 62 11 determinants determinant NNS cord-310467-v2wlu5eq 62 12 in in IN cord-310467-v2wlu5eq 62 13 the the DT cord-310467-v2wlu5eq 62 14 TGEV TGEV NNP cord-310467-v2wlu5eq 62 15 - - HYPH cord-310467-v2wlu5eq 62 16 infected infect VBN cord-310467-v2wlu5eq 62 17 cells cell NNS cord-310467-v2wlu5eq 62 18 and and CC cord-310467-v2wlu5eq 62 19 at at IN cord-310467-v2wlu5eq 62 20 different different JJ cord-310467-v2wlu5eq 62 21 temperatures temperature NNS cord-310467-v2wlu5eq 62 22 are be VBP cord-310467-v2wlu5eq 62 23 shown show VBN cord-310467-v2wlu5eq 62 24 in in IN cord-310467-v2wlu5eq 62 25 Fig Fig NNP cord-310467-v2wlu5eq 62 26 . . . cord-310467-v2wlu5eq 63 1 2 2 LS cord-310467-v2wlu5eq 63 2 . . . cord-310467-v2wlu5eq 64 1 Antibody antibody NN cord-310467-v2wlu5eq 64 2 binding bind VBG cord-310467-v2wlu5eq 64 3 to to IN cord-310467-v2wlu5eq 64 4 M M NNP cord-310467-v2wlu5eq 64 5 determinant determinant NN cord-310467-v2wlu5eq 64 6 was be VBD cord-310467-v2wlu5eq 64 7 decreased decrease VBN cord-310467-v2wlu5eq 64 8 whereas whereas IN cord-310467-v2wlu5eq 64 9 binding bind VBG cord-310467-v2wlu5eq 64 10 to to IN cord-310467-v2wlu5eq 64 11 S S NNP cord-310467-v2wlu5eq 64 12 and and CC cord-310467-v2wlu5eq 64 13 N n CD cord-310467-v2wlu5eq 64 14 determinants determinant NNS cord-310467-v2wlu5eq 64 15 increased increase VBD cord-310467-v2wlu5eq 64 16 in in IN cord-310467-v2wlu5eq 64 17 cells cell NNS cord-310467-v2wlu5eq 64 18 fixed fix VBN cord-310467-v2wlu5eq 64 19 with with IN cord-310467-v2wlu5eq 64 20 increasing increase VBG cord-310467-v2wlu5eq 64 21 acetone acetone NN cord-310467-v2wlu5eq 64 22 concentrations concentration NNS cord-310467-v2wlu5eq 64 23 at at IN cord-310467-v2wlu5eq 64 24 -20 -20 NNP cord-310467-v2wlu5eq 64 25 ° ° NNP cord-310467-v2wlu5eq 64 26 C C NNP cord-310467-v2wlu5eq 64 27 ( ( -LRB- cord-310467-v2wlu5eq 64 28 Fig Fig NNP cord-310467-v2wlu5eq 64 29 . . . cord-310467-v2wlu5eq 64 30 2a 2a CD cord-310467-v2wlu5eq 64 31 ) ) -RRB- cord-310467-v2wlu5eq 64 32 . . . cord-310467-v2wlu5eq 65 1 When when WRB cord-310467-v2wlu5eq 65 2 cell cell NN cord-310467-v2wlu5eq 65 3 fixation fixation NN cord-310467-v2wlu5eq 65 4 occurred occur VBD cord-310467-v2wlu5eq 65 5 at at IN cord-310467-v2wlu5eq 65 6 4 4 CD cord-310467-v2wlu5eq 65 7 ° ° NN cord-310467-v2wlu5eq 65 8 C c NN cord-310467-v2wlu5eq 65 9 , , , cord-310467-v2wlu5eq 65 10 an an DT cord-310467-v2wlu5eq 65 11 increase increase NN cord-310467-v2wlu5eq 65 12 in in IN cord-310467-v2wlu5eq 65 13 acetone acetone NN cord-310467-v2wlu5eq 65 14 concentration concentration NN cord-310467-v2wlu5eq 65 15 led lead VBD cord-310467-v2wlu5eq 65 16 to to IN cord-310467-v2wlu5eq 65 17 poor poor JJ cord-310467-v2wlu5eq 65 18 detection detection NN cord-310467-v2wlu5eq 65 19 of of IN cord-310467-v2wlu5eq 65 20 M M NNP cord-310467-v2wlu5eq 65 21 and and CC cord-310467-v2wlu5eq 65 22 S S NNP cord-310467-v2wlu5eq 65 23 determinants determinant NNS cord-310467-v2wlu5eq 65 24 although although IN cord-310467-v2wlu5eq 65 25 the the DT cord-310467-v2wlu5eq 65 26 latter latter JJ cord-310467-v2wlu5eq 65 27 could could MD cord-310467-v2wlu5eq 65 28 be be VB cord-310467-v2wlu5eq 65 29 detected detect VBN cord-310467-v2wlu5eq 65 30 in in IN cord-310467-v2wlu5eq 65 31 cells cell NNS cord-310467-v2wlu5eq 65 32 which which WDT cord-310467-v2wlu5eq 65 33 were be VBD cord-310467-v2wlu5eq 65 34 fixed fix VBN cord-310467-v2wlu5eq 65 35 with with IN cord-310467-v2wlu5eq 65 36 70 70 CD cord-310467-v2wlu5eq 65 37 % % NN cord-310467-v2wlu5eq 65 38 or or CC cord-310467-v2wlu5eq 65 39 80 80 CD cord-310467-v2wlu5eq 65 40 % % NN cord-310467-v2wlu5eq 65 41 acetone acetone NN cord-310467-v2wlu5eq 65 42 ( ( -LRB- cord-310467-v2wlu5eq 65 43 Fig Fig NNP cord-310467-v2wlu5eq 65 44 . . . cord-310467-v2wlu5eq 65 45 2b 2b NNP cord-310467-v2wlu5eq 65 46 ) ) -RRB- cord-310467-v2wlu5eq 65 47 . . . cord-310467-v2wlu5eq 66 1 In in IN cord-310467-v2wlu5eq 66 2 contrast contrast NN cord-310467-v2wlu5eq 66 3 , , , cord-310467-v2wlu5eq 66 4 the the DT cord-310467-v2wlu5eq 66 5 antibody antibody NN cord-310467-v2wlu5eq 66 6 binding bind VBG cord-310467-v2wlu5eq 66 7 to to IN cord-310467-v2wlu5eq 66 8 the the DT cord-310467-v2wlu5eq 66 9 N N NNP cord-310467-v2wlu5eq 66 10 ( ( -LRB- cord-310467-v2wlu5eq 66 11 22 22 CD cord-310467-v2wlu5eq 66 12 - - SYM cord-310467-v2wlu5eq 66 13 6 6 CD cord-310467-v2wlu5eq 66 14 ) ) -RRB- cord-310467-v2wlu5eq 66 15 determinant determinant NN cord-310467-v2wlu5eq 66 16 reached reach VBD cord-310467-v2wlu5eq 66 17 a a DT cord-310467-v2wlu5eq 66 18 plateau plateau NN cord-310467-v2wlu5eq 66 19 at at IN cord-310467-v2wlu5eq 66 20 40 40 CD cord-310467-v2wlu5eq 66 21 % % NN cord-310467-v2wlu5eq 66 22 . . . cord-310467-v2wlu5eq 67 1 The the DT cord-310467-v2wlu5eq 67 2 antibody antibody NN cord-310467-v2wlu5eq 67 3 binding bind VBG cord-310467-v2wlu5eq 67 4 profiles profile NNS cord-310467-v2wlu5eq 67 5 to to IN cord-310467-v2wlu5eq 67 6 the the DT cord-310467-v2wlu5eq 67 7 M M NNP cord-310467-v2wlu5eq 67 8 , , , cord-310467-v2wlu5eq 67 9 S S NNP cord-310467-v2wlu5eq 67 10 and and CC cord-310467-v2wlu5eq 67 11 N n CD cord-310467-v2wlu5eq 67 12 determinants determinant NNS cord-310467-v2wlu5eq 67 13 in in IN cord-310467-v2wlu5eq 67 14 cells cell NNS cord-310467-v2wlu5eq 67 15 fixed fix VBN cord-310467-v2wlu5eq 67 16 with with IN cord-310467-v2wlu5eq 67 17 acetone acetone NN cord-310467-v2wlu5eq 67 18 at at IN cord-310467-v2wlu5eq 67 19 RT RT NNP cord-310467-v2wlu5eq 67 20 showed show VBD cord-310467-v2wlu5eq 67 21 similarities similarity NNS cord-310467-v2wlu5eq 67 22 with with IN cord-310467-v2wlu5eq 67 23 the the DT cord-310467-v2wlu5eq 67 24 profiles profile NNS cord-310467-v2wlu5eq 67 25 obtained obtain VBN cord-310467-v2wlu5eq 67 26 in in IN cord-310467-v2wlu5eq 67 27 cell cell NN cord-310467-v2wlu5eq 67 28 fixation fixation NN cord-310467-v2wlu5eq 67 29 at at IN cord-310467-v2wlu5eq 67 30 4 4 CD cord-310467-v2wlu5eq 67 31 ° ° NN cord-310467-v2wlu5eq 67 32 C c NN cord-310467-v2wlu5eq 67 33 although although IN cord-310467-v2wlu5eq 67 34 their -PRON- PRP$ cord-310467-v2wlu5eq 67 35 OD OD NNP cord-310467-v2wlu5eq 67 36 values value NNS cord-310467-v2wlu5eq 67 37 were be VBD cord-310467-v2wlu5eq 67 38 lower low JJR cord-310467-v2wlu5eq 67 39 ( ( -LRB- cord-310467-v2wlu5eq 67 40 Fig Fig NNP cord-310467-v2wlu5eq 67 41 . . . cord-310467-v2wlu5eq 67 42 2c 2c NNP cord-310467-v2wlu5eq 67 43 ) ) -RRB- cord-310467-v2wlu5eq 67 44 . . . cord-310467-v2wlu5eq 68 1 In in IN cord-310467-v2wlu5eq 68 2 conclusion conclusion NN cord-310467-v2wlu5eq 68 3 , , , cord-310467-v2wlu5eq 68 4 M M NNP cord-310467-v2wlu5eq 68 5 and and CC cord-310467-v2wlu5eq 68 6 S S NNP cord-310467-v2wlu5eq 68 7 determinants determinant NNS cord-310467-v2wlu5eq 68 8 were be VBD cord-310467-v2wlu5eq 68 9 not not RB cord-310467-v2wlu5eq 68 10 well well RB cord-310467-v2wlu5eq 68 11 detected detect VBN cord-310467-v2wlu5eq 68 12 when when WRB cord-310467-v2wlu5eq 68 13 cell cell NN cord-310467-v2wlu5eq 68 14 fixation fixation NN cord-310467-v2wlu5eq 68 15 occurred occur VBD cord-310467-v2wlu5eq 68 16 at at IN cord-310467-v2wlu5eq 68 17 4 4 CD cord-310467-v2wlu5eq 68 18 ° ° NN cord-310467-v2wlu5eq 68 19 C c NN cord-310467-v2wlu5eq 68 20 and and CC cord-310467-v2wlu5eq 68 21 at at IN cord-310467-v2wlu5eq 68 22 RT RT NNP cord-310467-v2wlu5eq 68 23 while while IN cord-310467-v2wlu5eq 68 24 the the DT cord-310467-v2wlu5eq 68 25 N n CD cord-310467-v2wlu5eq 68 26 determinant determinant NN cord-310467-v2wlu5eq 68 27 was be VBD cord-310467-v2wlu5eq 68 28 well well RB cord-310467-v2wlu5eq 68 29 detected detect VBN cord-310467-v2wlu5eq 68 30 in in IN cord-310467-v2wlu5eq 68 31 cells cell NNS cord-310467-v2wlu5eq 68 32 fixed fix VBN cord-310467-v2wlu5eq 68 33 with with IN cord-310467-v2wlu5eq 68 34 high high JJ cord-310467-v2wlu5eq 68 35 acetone acetone NN cord-310467-v2wlu5eq 68 36 concentrations concentration NNS cord-310467-v2wlu5eq 68 37 regardless regardless RB cord-310467-v2wlu5eq 68 38 of of IN cord-310467-v2wlu5eq 68 39 the the DT cord-310467-v2wlu5eq 68 40 fixation fixation NN cord-310467-v2wlu5eq 68 41 temperature temperature NN cord-310467-v2wlu5eq 68 42 . . . cord-310467-v2wlu5eq 69 1 Fig Fig NNP cord-310467-v2wlu5eq 69 2 . . NNP cord-310467-v2wlu5eq 69 3 4 4 CD cord-310467-v2wlu5eq 69 4 . . . cord-310467-v2wlu5eq 70 1 The the DT cord-310467-v2wlu5eq 70 2 effect effect NN cord-310467-v2wlu5eq 70 3 of of IN cord-310467-v2wlu5eq 70 4 treating treat VBG cord-310467-v2wlu5eq 70 5 cells cell NNS cord-310467-v2wlu5eq 70 6 with with IN cord-310467-v2wlu5eq 70 7 0.05 0.05 CD cord-310467-v2wlu5eq 70 8 % % NN cord-310467-v2wlu5eq 70 9 glutaraldehyde glutaraldehyde NN cord-310467-v2wlu5eq 70 10 or or CC cord-310467-v2wlu5eq 70 11 0.1 0.1 CD cord-310467-v2wlu5eq 70 12 % % NN cord-310467-v2wlu5eq 70 13 paraformaldehyde paraformaldehyde NNP cord-310467-v2wlu5eq 70 14 and and CC cord-310467-v2wlu5eq 70 15 then then RB cord-310467-v2wlu5eq 70 16 with with IN cord-310467-v2wlu5eq 70 17 various various JJ cord-310467-v2wlu5eq 70 18 concentrations concentration NNS cord-310467-v2wlu5eq 70 19 of of IN cord-310467-v2wlu5eq 70 20 saponin saponin NN cord-310467-v2wlu5eq 70 21 on on IN cord-310467-v2wlu5eq 70 22 antibody antibody NN cord-310467-v2wlu5eq 70 23 binding bind VBG cord-310467-v2wlu5eq 70 24 to to IN cord-310467-v2wlu5eq 70 25 viral viral JJ cord-310467-v2wlu5eq 70 26 determinants determinant NNS cord-310467-v2wlu5eq 70 27 . . . cord-310467-v2wlu5eq 71 1 The the DT cord-310467-v2wlu5eq 71 2 degree degree NN cord-310467-v2wlu5eq 71 3 of of IN cord-310467-v2wlu5eq 71 4 antibody antibody NN cord-310467-v2wlu5eq 71 5 binding bind VBG cord-310467-v2wlu5eq 71 6 to to IN cord-310467-v2wlu5eq 71 7 the the DT cord-310467-v2wlu5eq 71 8 N N NNP cord-310467-v2wlu5eq 71 9 ( ( -LRB- cord-310467-v2wlu5eq 71 10 22 22 CD cord-310467-v2wlu5eq 71 11 - - SYM cord-310467-v2wlu5eq 71 12 6 6 CD cord-310467-v2wlu5eq 71 13 ) ) -RRB- cord-310467-v2wlu5eq 71 14 determinant determinant NN cord-310467-v2wlu5eq 71 15 was be VBD cord-310467-v2wlu5eq 71 16 expressed express VBN cord-310467-v2wlu5eq 71 17 as as IN cord-310467-v2wlu5eq 71 18 the the DT cord-310467-v2wlu5eq 71 19 optical optical JJ cord-310467-v2wlu5eq 71 20 density density NN cord-310467-v2wlu5eq 71 21 value value NN cord-310467-v2wlu5eq 71 22 obtained obtain VBN cord-310467-v2wlu5eq 71 23 with with IN cord-310467-v2wlu5eq 71 24 TGEV TGEV NNP cord-310467-v2wlu5eq 71 25 - - HYPH cord-310467-v2wlu5eq 71 26 infected infect VBN cord-310467-v2wlu5eq 71 27 ceils ceil NNS cord-310467-v2wlu5eq 71 28 prefixed prefix VBN cord-310467-v2wlu5eq 71 29 with with IN cord-310467-v2wlu5eq 71 30 the the DT cord-310467-v2wlu5eq 71 31 above above RB cord-310467-v2wlu5eq 71 32 - - HYPH cord-310467-v2wlu5eq 71 33 mentioned mention VBN cord-310467-v2wlu5eq 71 34 fixative fixative NN cord-310467-v2wlu5eq 71 35 preparation preparation NN cord-310467-v2wlu5eq 71 36 , , , cord-310467-v2wlu5eq 71 37 treated treat VBN cord-310467-v2wlu5eq 71 38 with with IN cord-310467-v2wlu5eq 71 39 saponin saponin NN cord-310467-v2wlu5eq 71 40 to to TO cord-310467-v2wlu5eq 71 41 permeate permeate VB cord-310467-v2wlu5eq 71 42 the the DT cord-310467-v2wlu5eq 71 43 cell cell NN cord-310467-v2wlu5eq 71 44 membrane membrane NN cord-310467-v2wlu5eq 71 45 and and CC cord-310467-v2wlu5eq 71 46 then then RB cord-310467-v2wlu5eq 71 47 incubated incubate VBN cord-310467-v2wlu5eq 71 48 with with IN cord-310467-v2wlu5eq 71 49 anti anti JJ cord-310467-v2wlu5eq 71 50 - - JJ cord-310467-v2wlu5eq 71 51 N n JJ cord-310467-v2wlu5eq 71 52 ( ( -LRB- cord-310467-v2wlu5eq 71 53 22 22 CD cord-310467-v2wlu5eq 71 54 - - SYM cord-310467-v2wlu5eq 71 55 6 6 CD cord-310467-v2wlu5eq 71 56 ) ) -RRB- cord-310467-v2wlu5eq 71 57 MAb MAb NNS cord-310467-v2wlu5eq 71 58 ( ( -LRB- cord-310467-v2wlu5eq 71 59 block block NN cord-310467-v2wlu5eq 71 60 1 1 CD cord-310467-v2wlu5eq 71 61 ) ) -RRB- cord-310467-v2wlu5eq 71 62 , , , cord-310467-v2wlu5eq 71 63 relative relative JJ cord-310467-v2wlu5eq 71 64 to to IN cord-310467-v2wlu5eq 71 65 the the DT cord-310467-v2wlu5eq 71 66 optical optical JJ cord-310467-v2wlu5eq 71 67 density density NN cord-310467-v2wlu5eq 71 68 obtained obtain VBN cord-310467-v2wlu5eq 71 69 with with IN cord-310467-v2wlu5eq 71 70 80 80 CD cord-310467-v2wlu5eq 71 71 % % NN cord-310467-v2wlu5eq 71 72 acetone acetone NN cord-310467-v2wlu5eq 71 73 - - HYPH cord-310467-v2wlu5eq 71 74 fixed fix VBN cord-310467-v2wlu5eq 71 75 , , , cord-310467-v2wlu5eq 71 76 TGEV TGEV NNP cord-310467-v2wlu5eq 71 77 - - HYPH cord-310467-v2wlu5eq 71 78 infected infect VBN cord-310467-v2wlu5eq 71 79 cells cell NNS cord-310467-v2wlu5eq 71 80 . . . cord-310467-v2wlu5eq 72 1 The the DT cord-310467-v2wlu5eq 72 2 degree degree NN cord-310467-v2wlu5eq 72 3 of of IN cord-310467-v2wlu5eq 72 4 antibody antibody NN cord-310467-v2wlu5eq 72 5 binding bind VBG cord-310467-v2wlu5eq 72 6 to to IN cord-310467-v2wlu5eq 72 7 the the DT cord-310467-v2wlu5eq 72 8 M M NNP cord-310467-v2wlu5eq 72 9 ( ( -LRB- cord-310467-v2wlu5eq 72 10 25 25 CD cord-310467-v2wlu5eq 72 11 - - SYM cord-310467-v2wlu5eq 72 12 22 22 CD cord-310467-v2wlu5eq 72 13 ) ) -RRB- cord-310467-v2wlu5eq 72 14 and and CC cord-310467-v2wlu5eq 72 15 S S NNP cord-310467-v2wlu5eq 72 16 ( ( -LRB- cord-310467-v2wlu5eq 72 17 51 51 CD cord-310467-v2wlu5eq 72 18 - - SYM cord-310467-v2wlu5eq 72 19 13 13 CD cord-310467-v2wlu5eq 72 20 ) ) -RRB- cord-310467-v2wlu5eq 72 21 determinants determinant NNS cord-310467-v2wlu5eq 72 22 was be VBD cord-310467-v2wlu5eq 72 23 expressed express VBN cord-310467-v2wlu5eq 72 24 as as IN cord-310467-v2wlu5eq 72 25 the the DT cord-310467-v2wlu5eq 72 26 optical optical JJ cord-310467-v2wlu5eq 72 27 density density NN cord-310467-v2wlu5eq 72 28 value value NN cord-310467-v2wlu5eq 72 29 obtained obtain VBN cord-310467-v2wlu5eq 72 30 with with IN cord-310467-v2wlu5eq 72 31 TGEV TGEV NNP cord-310467-v2wlu5eq 72 32 - - HYPH cord-310467-v2wlu5eq 72 33 infected infect VBN cord-310467-v2wlu5eq 72 34 cells cell NNS cord-310467-v2wlu5eq 72 35 prefixed prefix VBN cord-310467-v2wlu5eq 72 36 with with IN cord-310467-v2wlu5eq 72 37 above above RB cord-310467-v2wlu5eq 72 38 - - HYPH cord-310467-v2wlu5eq 72 39 mentioned mention VBN cord-310467-v2wlu5eq 72 40 fixative fixative NN cord-310467-v2wlu5eq 72 41 preparation preparation NN cord-310467-v2wlu5eq 72 42 , , , cord-310467-v2wlu5eq 72 43 treated treat VBN cord-310467-v2wlu5eq 72 44 with with IN cord-310467-v2wlu5eq 72 45 saponin saponin NN cord-310467-v2wlu5eq 72 46 and and CC cord-310467-v2wlu5eq 72 47 then then RB cord-310467-v2wlu5eq 72 48 incubated incubate VBD cord-310467-v2wlu5eq 72 49 with with IN cord-310467-v2wlu5eq 72 50 MAbs MAbs NNP cord-310467-v2wlu5eq 72 51 having have VBG cord-310467-v2wlu5eq 72 52 specificity specificity NN cord-310467-v2wlu5eq 72 53 for for IN cord-310467-v2wlu5eq 72 54 the the DT cord-310467-v2wlu5eq 72 55 M M NNP cord-310467-v2wlu5eq 72 56 ( ( -LRB- cord-310467-v2wlu5eq 72 57 25 25 CD cord-310467-v2wlu5eq 72 58 - - SYM cord-310467-v2wlu5eq 72 59 22 22 CD cord-310467-v2wlu5eq 72 60 ) ) -RRB- cord-310467-v2wlu5eq 72 61 ( ( -LRB- cord-310467-v2wlu5eq 72 62 block block NN cord-310467-v2wlu5eq 72 63 2 2 CD cord-310467-v2wlu5eq 72 64 ) ) -RRB- cord-310467-v2wlu5eq 72 65 and and CC cord-310467-v2wlu5eq 72 66 S S NNP cord-310467-v2wlu5eq 72 67 ( ( -LRB- cord-310467-v2wlu5eq 72 68 51 51 CD cord-310467-v2wlu5eq 72 69 - - SYM cord-310467-v2wlu5eq 72 70 13 13 CD cord-310467-v2wlu5eq 72 71 ) ) -RRB- cord-310467-v2wlu5eq 72 72 determinants determinant NNS cord-310467-v2wlu5eq 72 73 ( ( -LRB- cord-310467-v2wlu5eq 72 74 block block NN cord-310467-v2wlu5eq 72 75 3 3 CD cord-310467-v2wlu5eq 72 76 ) ) -RRB- cord-310467-v2wlu5eq 72 77 relative relative NN cord-310467-v2wlu5eq 72 78 to to IN cord-310467-v2wlu5eq 72 79 the the DT cord-310467-v2wlu5eq 72 80 optical optical JJ cord-310467-v2wlu5eq 72 81 densities density NNS cord-310467-v2wlu5eq 72 82 of of IN cord-310467-v2wlu5eq 72 83 0.1 0.1 CD cord-310467-v2wlu5eq 72 84 % % NN cord-310467-v2wlu5eq 72 85 paraformaldehyde paraformaldehyde NN cord-310467-v2wlu5eq 72 86 - - HYPH cord-310467-v2wlu5eq 72 87 fixed fix VBN cord-310467-v2wlu5eq 72 88 , , , cord-310467-v2wlu5eq 72 89 TGEV TGEV NNP cord-310467-v2wlu5eq 72 90 - - HYPH cord-310467-v2wlu5eq 72 91 infected infect VBN cord-310467-v2wlu5eq 72 92 cells cell NNS cord-310467-v2wlu5eq 72 93 . . . cord-310467-v2wlu5eq 73 1 determinant determinant NN cord-310467-v2wlu5eq 73 2 was be VBD cord-310467-v2wlu5eq 73 3 expressed express VBN cord-310467-v2wlu5eq 73 4 as as IN cord-310467-v2wlu5eq 73 5 the the DT cord-310467-v2wlu5eq 73 6 optical optical JJ cord-310467-v2wlu5eq 73 7 density density NN cord-310467-v2wlu5eq 73 8 value value NN cord-310467-v2wlu5eq 73 9 obtained obtain VBN cord-310467-v2wlu5eq 73 10 with with IN cord-310467-v2wlu5eq 73 11 TGEV TGEV NNP cord-310467-v2wlu5eq 73 12 - - HYPH cord-310467-v2wlu5eq 73 13 infectad infectad NN cord-310467-v2wlu5eq 73 14 cells cell NNS cord-310467-v2wlu5eq 73 15 prefLxed preflxe VBN cord-310467-v2wlu5eq 73 16 with with IN cord-310467-v2wlu5eq 73 17 the the DT cord-310467-v2wlu5eq 73 18 above above RB cord-310467-v2wlu5eq 73 19 - - HYPH cord-310467-v2wlu5eq 73 20 mentioned mention VBN cord-310467-v2wlu5eq 73 21 fixative fixative NN cord-310467-v2wlu5eq 73 22 preparation preparation NN cord-310467-v2wlu5eq 73 23 , , , cord-310467-v2wlu5eq 73 24 treated treat VBN cord-310467-v2wlu5eq 73 25 with with IN cord-310467-v2wlu5eq 73 26 Triton Triton NNP cord-310467-v2wlu5eq 73 27 X-100 X-100 NNS cord-310467-v2wlu5eq 73 28 to to TO cord-310467-v2wlu5eq 73 29 permeate permeate VB cord-310467-v2wlu5eq 73 30 the the DT cord-310467-v2wlu5eq 73 31 cell cell NN cord-310467-v2wlu5eq 73 32 membrane membrane NN cord-310467-v2wlu5eq 73 33 and and CC cord-310467-v2wlu5eq 73 34 then then RB cord-310467-v2wlu5eq 73 35 incubated incubate VBD cord-310467-v2wlu5eq 73 36 with with IN cord-310467-v2wlu5eq 73 37 a a DT cord-310467-v2wlu5eq 73 38 MAb MAb NNS cord-310467-v2wlu5eq 73 39 having have VBG cord-310467-v2wlu5eq 73 40 specificity specificity NN cord-310467-v2wlu5eq 73 41 for for IN cord-310467-v2wlu5eq 73 42 the the DT cord-310467-v2wlu5eq 73 43 N N NNP cord-310467-v2wlu5eq 73 44 ( ( -LRB- cord-310467-v2wlu5eq 73 45 22 22 CD cord-310467-v2wlu5eq 73 46 - - SYM cord-310467-v2wlu5eq 73 47 6 6 CD cord-310467-v2wlu5eq 73 48 ) ) -RRB- cord-310467-v2wlu5eq 73 49 determinant determinant NN cord-310467-v2wlu5eq 73 50 ( ( -LRB- cord-310467-v2wlu5eq 73 51 block block NN cord-310467-v2wlu5eq 73 52 1 1 CD cord-310467-v2wlu5eq 73 53 ) ) -RRB- cord-310467-v2wlu5eq 73 54 , , , cord-310467-v2wlu5eq 73 55 relative relative JJ cord-310467-v2wlu5eq 73 56 to to IN cord-310467-v2wlu5eq 73 57 the the DT cord-310467-v2wlu5eq 73 58 optical optical JJ cord-310467-v2wlu5eq 73 59 density density NN cord-310467-v2wlu5eq 73 60 obtained obtain VBN cord-310467-v2wlu5eq 73 61 with with IN cord-310467-v2wlu5eq 73 62 80 80 CD cord-310467-v2wlu5eq 73 63 % % NN cord-310467-v2wlu5eq 73 64 acetone acetone NN cord-310467-v2wlu5eq 73 65 - - HYPH cord-310467-v2wlu5eq 73 66 fixed fix VBN cord-310467-v2wlu5eq 73 67 , , , cord-310467-v2wlu5eq 73 68 TGEV TGEV NNP cord-310467-v2wlu5eq 73 69 - - HYPH cord-310467-v2wlu5eq 73 70 infected infect VBN cord-310467-v2wlu5eq 73 71 cells cell NNS cord-310467-v2wlu5eq 73 72 . . . cord-310467-v2wlu5eq 74 1 The the DT cord-310467-v2wlu5eq 74 2 degree degree NN cord-310467-v2wlu5eq 74 3 of of IN cord-310467-v2wlu5eq 74 4 antibody antibody NN cord-310467-v2wlu5eq 74 5 binding bind VBG cord-310467-v2wlu5eq 74 6 to to IN cord-310467-v2wlu5eq 74 7 the the DT cord-310467-v2wlu5eq 74 8 M M NNP cord-310467-v2wlu5eq 74 9 ( ( -LRB- cord-310467-v2wlu5eq 74 10 25 25 CD cord-310467-v2wlu5eq 74 11 - - SYM cord-310467-v2wlu5eq 74 12 22 22 CD cord-310467-v2wlu5eq 74 13 ) ) -RRB- cord-310467-v2wlu5eq 74 14 and and CC cord-310467-v2wlu5eq 74 15 S S NNP cord-310467-v2wlu5eq 74 16 ( ( -LRB- cord-310467-v2wlu5eq 74 17 51 51 CD cord-310467-v2wlu5eq 74 18 - - SYM cord-310467-v2wlu5eq 74 19 13 13 CD cord-310467-v2wlu5eq 74 20 ) ) -RRB- cord-310467-v2wlu5eq 74 21 determinants determinant NNS cord-310467-v2wlu5eq 74 22 was be VBD cord-310467-v2wlu5eq 74 23 expressed express VBN cord-310467-v2wlu5eq 74 24 as as IN cord-310467-v2wlu5eq 74 25 the the DT cord-310467-v2wlu5eq 74 26 optical optical JJ cord-310467-v2wlu5eq 74 27 density density NN cord-310467-v2wlu5eq 74 28 value value NN cord-310467-v2wlu5eq 74 29 obtained obtain VBN cord-310467-v2wlu5eq 74 30 with with IN cord-310467-v2wlu5eq 74 31 TGEV TGEV NNP cord-310467-v2wlu5eq 74 32 - - HYPH cord-310467-v2wlu5eq 74 33 infected infect VBN cord-310467-v2wlu5eq 74 34 cells cell NNS cord-310467-v2wlu5eq 74 35 prefixed prefix VBN cord-310467-v2wlu5eq 74 36 with with IN cord-310467-v2wlu5eq 74 37 above above RB cord-310467-v2wlu5eq 74 38 - - HYPH cord-310467-v2wlu5eq 74 39 mentioned mention VBN cord-310467-v2wlu5eq 74 40 fixative fixative NN cord-310467-v2wlu5eq 74 41 preparation preparation NN cord-310467-v2wlu5eq 74 42 , , , cord-310467-v2wlu5eq 74 43 treated treat VBN cord-310467-v2wlu5eq 74 44 with with IN cord-310467-v2wlu5eq 74 45 Triton Triton NNP cord-310467-v2wlu5eq 74 46 X-100 x-100 RB cord-310467-v2wlu5eq 74 47 and and CC cord-310467-v2wlu5eq 74 48 then then RB cord-310467-v2wlu5eq 74 49 incubated incubate VBD cord-310467-v2wlu5eq 74 50 with with IN cord-310467-v2wlu5eq 74 51 MAbs MAbs NNP cord-310467-v2wlu5eq 74 52 having have VBG cord-310467-v2wlu5eq 74 53 specificity specificity NN cord-310467-v2wlu5eq 74 54 for for IN cord-310467-v2wlu5eq 74 55 the the DT cord-310467-v2wlu5eq 74 56 M M NNP cord-310467-v2wlu5eq 74 57 ( ( -LRB- cord-310467-v2wlu5eq 74 58 25 25 CD cord-310467-v2wlu5eq 74 59 - - SYM cord-310467-v2wlu5eq 74 60 22 22 CD cord-310467-v2wlu5eq 74 61 ) ) -RRB- cord-310467-v2wlu5eq 74 62 determinant determinant NN cord-310467-v2wlu5eq 74 63 ( ( -LRB- cord-310467-v2wlu5eq 74 64 block block NN cord-310467-v2wlu5eq 74 65 2 2 CD cord-310467-v2wlu5eq 74 66 ) ) -RRB- cord-310467-v2wlu5eq 74 67 and and CC cord-310467-v2wlu5eq 74 68 S S NNP cord-310467-v2wlu5eq 74 69 ( ( -LRB- cord-310467-v2wlu5eq 74 70 51 51 CD cord-310467-v2wlu5eq 74 71 - - SYM cord-310467-v2wlu5eq 74 72 13 13 CD cord-310467-v2wlu5eq 74 73 ) ) -RRB- cord-310467-v2wlu5eq 74 74 determinants determinant NNS cord-310467-v2wlu5eq 74 75 ( ( -LRB- cord-310467-v2wlu5eq 74 76 block block NN cord-310467-v2wlu5eq 74 77 3 3 CD cord-310467-v2wlu5eq 74 78 ) ) -RRB- cord-310467-v2wlu5eq 75 1 relative relative JJ cord-310467-v2wlu5eq 75 2 to to IN cord-310467-v2wlu5eq 75 3 the the DT cord-310467-v2wlu5eq 75 4 optical optical JJ cord-310467-v2wlu5eq 75 5 densities density NNS cord-310467-v2wlu5eq 75 6 of of IN cord-310467-v2wlu5eq 75 7 0.1 0.1 CD cord-310467-v2wlu5eq 75 8 % % NN cord-310467-v2wlu5eq 75 9 paraformaldehyde paraformaldehyde NN cord-310467-v2wlu5eq 75 10 - - HYPH cord-310467-v2wlu5eq 75 11 fixed fix VBN cord-310467-v2wlu5eq 75 12 , , , cord-310467-v2wlu5eq 75 13 TGEV TGEV NNP cord-310467-v2wlu5eq 75 14 - - HYPH cord-310467-v2wlu5eq 75 15 infected infect VBN cord-310467-v2wlu5eq 75 16 cells cell NNS cord-310467-v2wlu5eq 75 17 . . . cord-310467-v2wlu5eq 76 1 As as IN cord-310467-v2wlu5eq 76 2 shown show VBN cord-310467-v2wlu5eq 76 3 in in IN cord-310467-v2wlu5eq 76 4 Fig Fig NNP cord-310467-v2wlu5eq 76 5 . . . cord-310467-v2wlu5eq 77 1 3 3 LS cord-310467-v2wlu5eq 77 2 , , , cord-310467-v2wlu5eq 77 3 the the DT cord-310467-v2wlu5eq 77 4 degree degree NN cord-310467-v2wlu5eq 77 5 of of IN cord-310467-v2wlu5eq 77 6 antibody antibody NN cord-310467-v2wlu5eq 77 7 binding bind VBG cord-310467-v2wlu5eq 77 8 to to IN cord-310467-v2wlu5eq 77 9 M M NNP cord-310467-v2wlu5eq 77 10 , , , cord-310467-v2wlu5eq 77 11 S S NNP cord-310467-v2wlu5eq 77 12 and and CC cord-310467-v2wlu5eq 77 13 N n CD cord-310467-v2wlu5eq 77 14 determinants determinant NNS cord-310467-v2wlu5eq 77 15 in in IN cord-310467-v2wlu5eq 77 16 methanol methanol NN cord-310467-v2wlu5eq 77 17 / / SYM cord-310467-v2wlu5eq 77 18 acetone acetone NN cord-310467-v2wlu5eq 77 19 / / SYM cord-310467-v2wlu5eq 77 20 ethanol ethanol NN cord-310467-v2wlu5eq 77 21 - - HYPH cord-310467-v2wlu5eq 77 22 and and CC cord-310467-v2wlu5eq 77 23 methanol methanol NN cord-310467-v2wlu5eq 77 24 / / SYM cord-310467-v2wlu5eq 77 25 acetone acetone NN cord-310467-v2wlu5eq 77 26 - - : cord-310467-v2wlu5eq 77 27 fLxed fLxed NNP cord-310467-v2wlu5eq 77 28 cells cell NNS cord-310467-v2wlu5eq 77 29 showed show VBD cord-310467-v2wlu5eq 77 30 similarities similarity NNS cord-310467-v2wlu5eq 77 31 with with IN cord-310467-v2wlu5eq 77 32 the the DT cord-310467-v2wlu5eq 77 33 results result NNS cord-310467-v2wlu5eq 77 34 obtained obtain VBN cord-310467-v2wlu5eq 77 35 in in IN cord-310467-v2wlu5eq 77 36 80 80 CD cord-310467-v2wlu5eq 77 37 % % NN cord-310467-v2wlu5eq 77 38 acetone acetone NN cord-310467-v2wlu5eq 77 39 - - HYPH cord-310467-v2wlu5eq 77 40 fixed fix VBN cord-310467-v2wlu5eq 77 41 cells cell NNS cord-310467-v2wlu5eq 77 42 . . . cord-310467-v2wlu5eq 78 1 The the DT cord-310467-v2wlu5eq 78 2 extent extent NN cord-310467-v2wlu5eq 78 3 of of IN cord-310467-v2wlu5eq 78 4 antibody antibody NN cord-310467-v2wlu5eq 78 5 binding bind VBG cord-310467-v2wlu5eq 78 6 to to IN cord-310467-v2wlu5eq 78 7 these these DT cord-310467-v2wlu5eq 78 8 determinants determinant NNS cord-310467-v2wlu5eq 78 9 in in IN cord-310467-v2wlu5eq 78 10 methanol methanol NN cord-310467-v2wlu5eq 78 11 / / SYM cord-310467-v2wlu5eq 78 12 acetone acetone NN cord-310467-v2wlu5eq 78 13 / / SYM cord-310467-v2wlu5eq 78 14 FA- fa- NN cord-310467-v2wlu5eq 78 15 , , , cord-310467-v2wlu5eq 79 1 absolute absolute JJ cord-310467-v2wlu5eq 79 2 methanol- methanol- NN cord-310467-v2wlu5eq 79 3 , , , cord-310467-v2wlu5eq 79 4 ethanol ethanol NN cord-310467-v2wlu5eq 79 5 / / SYM cord-310467-v2wlu5eq 79 6 acetic acetic NN cord-310467-v2wlu5eq 79 7 acid- acid- NN cord-310467-v2wlu5eq 79 8 , , , cord-310467-v2wlu5eq 80 1 ethanol ethanol NN cord-310467-v2wlu5eq 80 2 / / SYM cord-310467-v2wlu5eq 80 3 ether- ether- XX cord-310467-v2wlu5eq 81 1 , , , cord-310467-v2wlu5eq 81 2 absolute absolute JJ cord-310467-v2wlu5eq 81 3 ethanol ethanol NN cord-310467-v2wlu5eq 81 4 - - HYPH cord-310467-v2wlu5eq 81 5 fixed fix VBN cord-310467-v2wlu5eq 81 6 cells cell NNS cord-310467-v2wlu5eq 81 7 was be VBD cord-310467-v2wlu5eq 81 8 lower low JJR cord-310467-v2wlu5eq 81 9 than than IN cord-310467-v2wlu5eq 81 10 that that DT cord-310467-v2wlu5eq 81 11 detected detect VBN cord-310467-v2wlu5eq 81 12 in in IN cord-310467-v2wlu5eq 81 13 80 80 CD cord-310467-v2wlu5eq 81 14 % % NN cord-310467-v2wlu5eq 81 15 acetone acetone NN cord-310467-v2wlu5eq 81 16 - - HYPH cord-310467-v2wlu5eq 81 17 fixed fix VBN cord-310467-v2wlu5eq 81 18 cells cell NNS cord-310467-v2wlu5eq 81 19 . . . cord-310467-v2wlu5eq 82 1 It -PRON- PRP cord-310467-v2wlu5eq 82 2 can can MD cord-310467-v2wlu5eq 82 3 be be VB cord-310467-v2wlu5eq 82 4 noted note VBN cord-310467-v2wlu5eq 82 5 that that IN cord-310467-v2wlu5eq 82 6 antibody antibody NN cord-310467-v2wlu5eq 82 7 binding bind VBG cord-310467-v2wlu5eq 82 8 to to IN cord-310467-v2wlu5eq 82 9 the the DT cord-310467-v2wlu5eq 82 10 M M NNP cord-310467-v2wlu5eq 82 11 and and CC cord-310467-v2wlu5eq 82 12 S S NNP cord-310467-v2wlu5eq 82 13 determinants determinant NNS cord-310467-v2wlu5eq 82 14 in in IN cord-310467-v2wlu5eq 82 15 0.1 0.1 CD cord-310467-v2wlu5eq 82 16 % % NN cord-310467-v2wlu5eq 82 17 PFA PFA NNP cord-310467-v2wlu5eq 82 18 - - HYPH cord-310467-v2wlu5eq 82 19 fixed fix VBN cord-310467-v2wlu5eq 82 20 , , , cord-310467-v2wlu5eq 82 21 TGEV TGEV NNP cord-310467-v2wlu5eq 82 22 - - HYPH cord-310467-v2wlu5eq 82 23 infected infect VBN cord-310467-v2wlu5eq 82 24 cells cell NNS cord-310467-v2wlu5eq 82 25 was be VBD cord-310467-v2wlu5eq 82 26 greater great JJR cord-310467-v2wlu5eq 82 27 than than IN cord-310467-v2wlu5eq 82 28 those those DT cord-310467-v2wlu5eq 82 29 of of IN cord-310467-v2wlu5eq 82 30 cells cell NNS cord-310467-v2wlu5eq 82 31 fixed fix VBN cord-310467-v2wlu5eq 82 32 with with IN cord-310467-v2wlu5eq 82 33 any any DT cord-310467-v2wlu5eq 82 34 of of IN cord-310467-v2wlu5eq 82 35 these these DT cord-310467-v2wlu5eq 82 36 dehydrating dehydrate VBG cord-310467-v2wlu5eq 82 37 fixatives fixative NNS cord-310467-v2wlu5eq 82 38 . . . cord-310467-v2wlu5eq 83 1 Fig Fig NNP cord-310467-v2wlu5eq 83 2 . . NNP cord-310467-v2wlu5eq 83 3 6 6 CD cord-310467-v2wlu5eq 83 4 . . . cord-310467-v2wlu5eq 84 1 The the DT cord-310467-v2wlu5eq 84 2 comparative comparative JJ cord-310467-v2wlu5eq 84 3 effect effect NN cord-310467-v2wlu5eq 84 4 of of IN cord-310467-v2wlu5eq 84 5 treating treat VBG cord-310467-v2wlu5eq 84 6 cells cell NNS cord-310467-v2wlu5eq 84 7 with with IN cord-310467-v2wlu5eq 84 8 0.1 0.1 CD cord-310467-v2wlu5eq 84 9 % % NN cord-310467-v2wlu5eq 84 10 paraformaldehyde paraformaldehyde NN cord-310467-v2wlu5eq 84 11 and and CC cord-310467-v2wlu5eq 84 12 then then RB cord-310467-v2wlu5eq 84 13 with with IN cord-310467-v2wlu5eq 84 14 different different JJ cord-310467-v2wlu5eq 84 15 detergents detergent NNS cord-310467-v2wlu5eq 84 16 on on IN cord-310467-v2wlu5eq 84 17 antibody antibody NN cord-310467-v2wlu5eq 84 18 binding bind VBG cord-310467-v2wlu5eq 84 19 to to IN cord-310467-v2wlu5eq 84 20 the the DT cord-310467-v2wlu5eq 84 21 viral viral JJ cord-310467-v2wlu5eq 84 22 determinants determinant NNS cord-310467-v2wlu5eq 84 23 . . . cord-310467-v2wlu5eq 85 1 The the DT cord-310467-v2wlu5eq 85 2 degree degree NN cord-310467-v2wlu5eq 85 3 of of IN cord-310467-v2wlu5eq 85 4 antibody antibody NN cord-310467-v2wlu5eq 85 5 binding bind VBG cord-310467-v2wlu5eq 85 6 to to IN cord-310467-v2wlu5eq 85 7 the the DT cord-310467-v2wlu5eq 85 8 N N NNP cord-310467-v2wlu5eq 85 9 ( ( -LRB- cord-310467-v2wlu5eq 85 10 22 22 CD cord-310467-v2wlu5eq 85 11 - - SYM cord-310467-v2wlu5eq 85 12 6 6 CD cord-310467-v2wlu5eq 85 13 ) ) -RRB- cord-310467-v2wlu5eq 85 14 determinant determinant NN cord-310467-v2wlu5eq 85 15 was be VBD cord-310467-v2wlu5eq 85 16 expressed express VBN cord-310467-v2wlu5eq 85 17 as as IN cord-310467-v2wlu5eq 85 18 the the DT cord-310467-v2wlu5eq 85 19 optical optical JJ cord-310467-v2wlu5eq 85 20 density density NN cord-310467-v2wlu5eq 85 21 value value NN cord-310467-v2wlu5eq 85 22 obtained obtain VBN cord-310467-v2wlu5eq 85 23 with with IN cord-310467-v2wlu5eq 85 24 TGEV TGEV NNP cord-310467-v2wlu5eq 85 25 - - HYPH cord-310467-v2wlu5eq 85 26 infected infect VBN cord-310467-v2wlu5eq 85 27 cells cell NNS cord-310467-v2wlu5eq 85 28 prefixed prefix VBN cord-310467-v2wlu5eq 85 29 with with IN cord-310467-v2wlu5eq 85 30 above above RB cord-310467-v2wlu5eq 85 31 - - HYPH cord-310467-v2wlu5eq 85 32 mentioned mention VBN cord-310467-v2wlu5eq 85 33 fixative fixative NN cord-310467-v2wlu5eq 85 34 preparation preparation NN cord-310467-v2wlu5eq 85 35 , , , cord-310467-v2wlu5eq 85 36 treated treat VBN cord-310467-v2wlu5eq 85 37 with with IN cord-310467-v2wlu5eq 85 38 indicated indicate VBN cord-310467-v2wlu5eq 85 39 detergent detergent NN cord-310467-v2wlu5eq 85 40 to to TO cord-310467-v2wlu5eq 85 41 perorate perorate VB cord-310467-v2wlu5eq 85 42 the the DT cord-310467-v2wlu5eq 85 43 cell cell NN cord-310467-v2wlu5eq 85 44 membrane membrane NN cord-310467-v2wlu5eq 85 45 and and CC cord-310467-v2wlu5eq 85 46 then then RB cord-310467-v2wlu5eq 85 47 incubated incubate VBD cord-310467-v2wlu5eq 85 48 with with IN cord-310467-v2wlu5eq 85 49 a a DT cord-310467-v2wlu5eq 85 50 MAb MAb NNS cord-310467-v2wlu5eq 85 51 having have VBG cord-310467-v2wlu5eq 85 52 specificity specificity NN cord-310467-v2wlu5eq 85 53 for for IN cord-310467-v2wlu5eq 85 54 the the DT cord-310467-v2wlu5eq 85 55 N N NNP cord-310467-v2wlu5eq 85 56 ( ( -LRB- cord-310467-v2wlu5eq 85 57 22 22 CD cord-310467-v2wlu5eq 85 58 - - SYM cord-310467-v2wlu5eq 85 59 6 6 CD cord-310467-v2wlu5eq 85 60 ) ) -RRB- cord-310467-v2wlu5eq 85 61 determinant determinant NN cord-310467-v2wlu5eq 85 62 ( ( -LRB- cord-310467-v2wlu5eq 85 63 block block NN cord-310467-v2wlu5eq 85 64 1 1 CD cord-310467-v2wlu5eq 85 65 ) ) -RRB- cord-310467-v2wlu5eq 85 66 , , , cord-310467-v2wlu5eq 85 67 relative relative JJ cord-310467-v2wlu5eq 85 68 to to IN cord-310467-v2wlu5eq 85 69 the the DT cord-310467-v2wlu5eq 85 70 optical optical JJ cord-310467-v2wlu5eq 85 71 density density NN cord-310467-v2wlu5eq 85 72 obtained obtain VBN cord-310467-v2wlu5eq 85 73 with with IN cord-310467-v2wlu5eq 85 74 80 80 CD cord-310467-v2wlu5eq 85 75 % % NN cord-310467-v2wlu5eq 85 76 acetone acetone NN cord-310467-v2wlu5eq 85 77 - - HYPH cord-310467-v2wlu5eq 85 78 fixed fix VBN cord-310467-v2wlu5eq 85 79 , , , cord-310467-v2wlu5eq 85 80 TGEV TGEV NNP cord-310467-v2wlu5eq 85 81 - - HYPH cord-310467-v2wlu5eq 85 82 infected infect VBN cord-310467-v2wlu5eq 85 83 cells cell NNS cord-310467-v2wlu5eq 85 84 . . . cord-310467-v2wlu5eq 86 1 The the DT cord-310467-v2wlu5eq 86 2 degree degree NN cord-310467-v2wlu5eq 86 3 of of IN cord-310467-v2wlu5eq 86 4 antibody antibody NN cord-310467-v2wlu5eq 86 5 binding bind VBG cord-310467-v2wlu5eq 86 6 to to IN cord-310467-v2wlu5eq 86 7 the the DT cord-310467-v2wlu5eq 86 8 M M NNP cord-310467-v2wlu5eq 86 9 ( ( -LRB- cord-310467-v2wlu5eq 86 10 25 25 CD cord-310467-v2wlu5eq 86 11 - - SYM cord-310467-v2wlu5eq 86 12 22 22 CD cord-310467-v2wlu5eq 86 13 ) ) -RRB- cord-310467-v2wlu5eq 86 14 and and CC cord-310467-v2wlu5eq 86 15 S S NNP cord-310467-v2wlu5eq 86 16 ( ( -LRB- cord-310467-v2wlu5eq 86 17 51 51 CD cord-310467-v2wlu5eq 86 18 - - SYM cord-310467-v2wlu5eq 86 19 13 13 CD cord-310467-v2wlu5eq 86 20 ) ) -RRB- cord-310467-v2wlu5eq 86 21 determinants determinant NNS cord-310467-v2wlu5eq 86 22 was be VBD cord-310467-v2wlu5eq 86 23 expressed express VBN cord-310467-v2wlu5eq 86 24 as as IN cord-310467-v2wlu5eq 86 25 the the DT cord-310467-v2wlu5eq 86 26 optical optical JJ cord-310467-v2wlu5eq 86 27 density density NN cord-310467-v2wlu5eq 86 28 value value NN cord-310467-v2wlu5eq 86 29 obtained obtain VBN cord-310467-v2wlu5eq 86 30 with with IN cord-310467-v2wlu5eq 86 31 TGEV TGEV NNP cord-310467-v2wlu5eq 86 32 - - HYPH cord-310467-v2wlu5eq 86 33 infected infect VBN cord-310467-v2wlu5eq 86 34 cells cell NNS cord-310467-v2wlu5eq 86 35 prefixed prefix VBN cord-310467-v2wlu5eq 86 36 with with IN cord-310467-v2wlu5eq 86 37 above above RB cord-310467-v2wlu5eq 86 38 - - HYPH cord-310467-v2wlu5eq 86 39 mentioned mention VBN cord-310467-v2wlu5eq 86 40 fixative fixative NN cord-310467-v2wlu5eq 86 41 preparation preparation NN cord-310467-v2wlu5eq 86 42 , , , cord-310467-v2wlu5eq 86 43 treated treat VBN cord-310467-v2wlu5eq 86 44 with with IN cord-310467-v2wlu5eq 86 45 detergetit detergetit NNP cord-310467-v2wlu5eq 86 46 and and CC cord-310467-v2wlu5eq 86 47 then then RB cord-310467-v2wlu5eq 86 48 incubated incubate VBD cord-310467-v2wlu5eq 86 49 with with IN cord-310467-v2wlu5eq 86 50 MAbs MAbs NNP cord-310467-v2wlu5eq 86 51 having have VBG cord-310467-v2wlu5eq 86 52 specificity specificity NN cord-310467-v2wlu5eq 86 53 for for IN cord-310467-v2wlu5eq 86 54 the the DT cord-310467-v2wlu5eq 86 55 M M NNP cord-310467-v2wlu5eq 86 56 ( ( -LRB- cord-310467-v2wlu5eq 86 57 25 25 CD cord-310467-v2wlu5eq 86 58 - - SYM cord-310467-v2wlu5eq 86 59 22 22 CD cord-310467-v2wlu5eq 86 60 ) ) -RRB- cord-310467-v2wlu5eq 86 61 ( ( -LRB- cord-310467-v2wlu5eq 86 62 block block NN cord-310467-v2wlu5eq 86 63 2 2 CD cord-310467-v2wlu5eq 86 64 ) ) -RRB- cord-310467-v2wlu5eq 86 65 and and CC cord-310467-v2wlu5eq 86 66 S S NNP cord-310467-v2wlu5eq 86 67 ( ( -LRB- cord-310467-v2wlu5eq 86 68 51 51 CD cord-310467-v2wlu5eq 86 69 - - SYM cord-310467-v2wlu5eq 86 70 13 13 CD cord-310467-v2wlu5eq 86 71 ) ) -RRB- cord-310467-v2wlu5eq 86 72 determinants determinant NNS cord-310467-v2wlu5eq 86 73 ( ( -LRB- cord-310467-v2wlu5eq 86 74 block block NN cord-310467-v2wlu5eq 86 75 3 3 CD cord-310467-v2wlu5eq 86 76 ) ) -RRB- cord-310467-v2wlu5eq 86 77 relative relative NN cord-310467-v2wlu5eq 86 78 to to IN cord-310467-v2wlu5eq 86 79 the the DT cord-310467-v2wlu5eq 86 80 optical optical JJ cord-310467-v2wlu5eq 86 81 densities density NNS cord-310467-v2wlu5eq 86 82 of of IN cord-310467-v2wlu5eq 86 83 0.1 0.1 CD cord-310467-v2wlu5eq 86 84 % % NN cord-310467-v2wlu5eq 86 85 paraformaldehyde paraformaldehyde NN cord-310467-v2wlu5eq 86 86 - - HYPH cord-310467-v2wlu5eq 86 87 fixed fix VBN cord-310467-v2wlu5eq 86 88 , , , cord-310467-v2wlu5eq 86 89 TGEV TGEV NNP cord-310467-v2wlu5eq 86 90 - - HYPH cord-310467-v2wlu5eq 86 91 infected infect VBN cord-310467-v2wlu5eq 86 92 cells cell NNS cord-310467-v2wlu5eq 86 93 . . . cord-310467-v2wlu5eq 87 1 The the DT cord-310467-v2wlu5eq 87 2 degrees degree NNS cord-310467-v2wlu5eq 87 3 of of IN cord-310467-v2wlu5eq 87 4 antibody antibody NN cord-310467-v2wlu5eq 87 5 binding bind VBG cord-310467-v2wlu5eq 87 6 to to IN cord-310467-v2wlu5eq 87 7 viral viral JJ cord-310467-v2wlu5eq 87 8 determinants determinant NNS cord-310467-v2wlu5eq 87 9 in in IN cord-310467-v2wlu5eq 87 10 cells cell NNS cord-310467-v2wlu5eq 87 11 which which WDT cord-310467-v2wlu5eq 87 12 were be VBD cord-310467-v2wlu5eq 87 13 fixed fix VBN cord-310467-v2wlu5eq 87 14 with with IN cord-310467-v2wlu5eq 87 15 the the DT cord-310467-v2wlu5eq 87 16 aidehyde aidehyde NNP cord-310467-v2wlu5eq 87 17 fixative fixative NN cord-310467-v2wlu5eq 87 18 , , , cord-310467-v2wlu5eq 87 19 e.g. e.g. RB cord-310467-v2wlu5eq 87 20 , , , cord-310467-v2wlu5eq 87 21 0.05 0.05 CD cord-310467-v2wlu5eq 87 22 % % NN cord-310467-v2wlu5eq 87 23 GA GA NNP cord-310467-v2wlu5eq 87 24 or or CC cord-310467-v2wlu5eq 87 25 0.1 0.1 CD cord-310467-v2wlu5eq 87 26 % % NN cord-310467-v2wlu5eq 87 27 PFA PFA NNP cord-310467-v2wlu5eq 87 28 and and CC cord-310467-v2wlu5eq 87 29 then then RB cord-310467-v2wlu5eq 87 30 permeated permeate VBD cord-310467-v2wlu5eq 87 31 with with IN cord-310467-v2wlu5eq 87 32 different different JJ cord-310467-v2wlu5eq 87 33 concentrations concentration NNS cord-310467-v2wlu5eq 87 34 of of IN cord-310467-v2wlu5eq 87 35 saponin saponin NN cord-310467-v2wlu5eq 87 36 are be VBP cord-310467-v2wlu5eq 87 37 shown show VBN cord-310467-v2wlu5eq 87 38 in in IN cord-310467-v2wlu5eq 87 39 Fig Fig NNP cord-310467-v2wlu5eq 87 40 . . . cord-310467-v2wlu5eq 88 1 4 4 LS cord-310467-v2wlu5eq 88 2 . . . cord-310467-v2wlu5eq 89 1 The the DT cord-310467-v2wlu5eq 89 2 degree degree NN cord-310467-v2wlu5eq 89 3 of of IN cord-310467-v2wlu5eq 89 4 antibody antibody NN cord-310467-v2wlu5eq 89 5 binding bind VBG cord-310467-v2wlu5eq 89 6 to to IN cord-310467-v2wlu5eq 89 7 the the DT cord-310467-v2wlu5eq 89 8 M M NNP cord-310467-v2wlu5eq 89 9 , , , cord-310467-v2wlu5eq 89 10 S S NNP cord-310467-v2wlu5eq 89 11 and and CC cord-310467-v2wlu5eq 89 12 N n CD cord-310467-v2wlu5eq 89 13 determinants determinant NNS cord-310467-v2wlu5eq 89 14 in in IN cord-310467-v2wlu5eq 89 15 0.05 0.05 CD cord-310467-v2wlu5eq 89 16 % % NN cord-310467-v2wlu5eq 89 17 GA GA NNP cord-310467-v2wlu5eq 89 18 - - HYPH cord-310467-v2wlu5eq 89 19 fixed fix VBN cord-310467-v2wlu5eq 89 20 cells cell NNS cord-310467-v2wlu5eq 89 21 which which WDT cord-310467-v2wlu5eq 89 22 were be VBD cord-310467-v2wlu5eq 89 23 permeated permeate VBN cord-310467-v2wlu5eq 89 24 with with IN cord-310467-v2wlu5eq 89 25 0.1 0.1 CD cord-310467-v2wlu5eq 89 26 % % NN cord-310467-v2wlu5eq 89 27 saponin saponin NN cord-310467-v2wlu5eq 89 28 was be VBD cord-310467-v2wlu5eq 89 29 similar similar JJ cord-310467-v2wlu5eq 89 30 to to IN cord-310467-v2wlu5eq 89 31 that that DT cord-310467-v2wlu5eq 90 1 ob ob NNP cord-310467-v2wlu5eq 90 2 / / SYM cord-310467-v2wlu5eq 90 3 ained aine VBD cord-310467-v2wlu5eq 90 4 in in IN cord-310467-v2wlu5eq 90 5 80 80 CD cord-310467-v2wlu5eq 90 6 % % NN cord-310467-v2wlu5eq 90 7 acetone acetone NN cord-310467-v2wlu5eq 90 8 - - HYPH cord-310467-v2wlu5eq 90 9 fixed fix VBN cord-310467-v2wlu5eq 90 10 cells cell NNS cord-310467-v2wlu5eq 90 11 . . . cord-310467-v2wlu5eq 91 1 With with IN cord-310467-v2wlu5eq 91 2 the the DT cord-310467-v2wlu5eq 91 3 exception exception NN cord-310467-v2wlu5eq 91 4 of of IN cord-310467-v2wlu5eq 91 5 the the DT cord-310467-v2wlu5eq 91 6 0.1 0.1 CD cord-310467-v2wlu5eq 91 7 % % NN cord-310467-v2wlu5eq 91 8 PFA PFA NNP cord-310467-v2wlu5eq 91 9 control control VBP cord-310467-v2wlu5eq 91 10 the the DT cord-310467-v2wlu5eq 91 11 degree degree NN cord-310467-v2wlu5eq 91 12 of of IN cord-310467-v2wlu5eq 91 13 antibody antibody NN cord-310467-v2wlu5eq 91 14 binding bind VBG cord-310467-v2wlu5eq 91 15 to to IN cord-310467-v2wlu5eq 91 16 all all DT cord-310467-v2wlu5eq 91 17 other other JJ cord-310467-v2wlu5eq 91 18 samples sample NNS cord-310467-v2wlu5eq 91 19 was be VBD cord-310467-v2wlu5eq 91 20 lower low JJR cord-310467-v2wlu5eq 91 21 than than IN cord-310467-v2wlu5eq 91 22 that that DT cord-310467-v2wlu5eq 91 23 detected detect VBN cord-310467-v2wlu5eq 91 24 in in IN cord-310467-v2wlu5eq 91 25 80 80 CD cord-310467-v2wlu5eq 91 26 % % NN cord-310467-v2wlu5eq 91 27 acetone acetone NN cord-310467-v2wlu5eq 91 28 - - HYPH cord-310467-v2wlu5eq 91 29 fixed fix VBN cord-310467-v2wlu5eq 91 30 cells cell NNS cord-310467-v2wlu5eq 91 31 . . . cord-310467-v2wlu5eq 92 1 When when WRB cord-310467-v2wlu5eq 92 2 using use VBG cord-310467-v2wlu5eq 92 3 Triton Triton NNP cord-310467-v2wlu5eq 92 4 X-100 x-100 IN cord-310467-v2wlu5eq 92 5 ( ( -LRB- cord-310467-v2wlu5eq 92 6 Fig Fig NNP cord-310467-v2wlu5eq 92 7 . . . cord-310467-v2wlu5eq 92 8 5 5 CD cord-310467-v2wlu5eq 92 9 ) ) -RRB- cord-310467-v2wlu5eq 92 10 , , , cord-310467-v2wlu5eq 92 11 antibody antibody NN cord-310467-v2wlu5eq 92 12 binding bind VBG cord-310467-v2wlu5eq 92 13 to to IN cord-310467-v2wlu5eq 92 14 the the DT cord-310467-v2wlu5eq 92 15 M M NNP cord-310467-v2wlu5eq 92 16 and and CC cord-310467-v2wlu5eq 92 17 N n CD cord-310467-v2wlu5eq 92 18 determinants determinant NNS cord-310467-v2wlu5eq 92 19 in in IN cord-310467-v2wlu5eq 92 20 0.05 0.05 CD cord-310467-v2wlu5eq 92 21 % % NN cord-310467-v2wlu5eq 92 22 GA GA NNP cord-310467-v2wlu5eq 92 23 - - : cord-310467-v2wlu5eq 92 24 or or CC cord-310467-v2wlu5eq 92 25 0.1 0.1 CD cord-310467-v2wlu5eq 92 26 % % NN cord-310467-v2wlu5eq 92 27 PFA PFA NNP cord-310467-v2wlu5eq 92 28 - - HYPH cord-310467-v2wlu5eq 92 29 fixed fix VBN cord-310467-v2wlu5eq 92 30 cells cell NNS cord-310467-v2wlu5eq 92 31 which which WDT cord-310467-v2wlu5eq 92 32 were be VBD cord-310467-v2wlu5eq 92 33 then then RB cord-310467-v2wlu5eq 92 34 permeated permeate VBN cord-310467-v2wlu5eq 92 35 with with IN cord-310467-v2wlu5eq 92 36 Triton Triton NNP cord-310467-v2wlu5eq 92 37 X-100 x-100 RB cord-310467-v2wlu5eq 92 38 at at IN cord-310467-v2wlu5eq 92 39 various various JJ cord-310467-v2wlu5eq 92 40 concentrations concentration NNS cord-310467-v2wlu5eq 92 41 showed show VBD cord-310467-v2wlu5eq 92 42 similarities similarity NNS cord-310467-v2wlu5eq 92 43 with with IN cord-310467-v2wlu5eq 92 44 the the DT cord-310467-v2wlu5eq 92 45 results result NNS cord-310467-v2wlu5eq 92 46 obtained obtain VBN cord-310467-v2wlu5eq 92 47 in in IN cord-310467-v2wlu5eq 92 48 80 80 CD cord-310467-v2wlu5eq 92 49 % % NN cord-310467-v2wlu5eq 92 50 acetone acetone NN cord-310467-v2wlu5eq 92 51 - - HYPH cord-310467-v2wlu5eq 92 52 fixed fix VBN cord-310467-v2wlu5eq 92 53 cells cell NNS cord-310467-v2wlu5eq 92 54 . . . cord-310467-v2wlu5eq 93 1 The the DT cord-310467-v2wlu5eq 93 2 degree degree NN cord-310467-v2wlu5eq 93 3 of of IN cord-310467-v2wlu5eq 93 4 antibody antibody NN cord-310467-v2wlu5eq 93 5 binding bind VBG cord-310467-v2wlu5eq 93 6 to to IN cord-310467-v2wlu5eq 93 7 the the DT cord-310467-v2wlu5eq 93 8 S S NNP cord-310467-v2wlu5eq 93 9 determinant determinant NN cord-310467-v2wlu5eq 93 10 in in IN cord-310467-v2wlu5eq 93 11 0.05 0.05 CD cord-310467-v2wlu5eq 93 12 % % NN cord-310467-v2wlu5eq 93 13 GA GA NNP cord-310467-v2wlu5eq 93 14 - - HYPH cord-310467-v2wlu5eq 93 15 fixed fix VBN cord-310467-v2wlu5eq 93 16 cells cell NNS cord-310467-v2wlu5eq 93 17 which which WDT cord-310467-v2wlu5eq 93 18 were be VBD cord-310467-v2wlu5eq 93 19 then then RB cord-310467-v2wlu5eq 93 20 permeated permeate VBN cord-310467-v2wlu5eq 93 21 with with IN cord-310467-v2wlu5eq 93 22 Triton Triton NNP cord-310467-v2wlu5eq 93 23 X-100 x-100 RB cord-310467-v2wlu5eq 93 24 at at IN cord-310467-v2wlu5eq 93 25 1.0 1.0 CD cord-310467-v2wlu5eq 93 26 , , , cord-310467-v2wlu5eq 93 27 0.75 0.75 CD cord-310467-v2wlu5eq 93 28 and and CC cord-310467-v2wlu5eq 93 29 0.5 0.5 CD cord-310467-v2wlu5eq 93 30 % % NN cord-310467-v2wlu5eq 93 31 was be VBD cord-310467-v2wlu5eq 93 32 lower low JJR cord-310467-v2wlu5eq 93 33 than than IN cord-310467-v2wlu5eq 93 34 that that DT cord-310467-v2wlu5eq 93 35 obtained obtain VBN cord-310467-v2wlu5eq 93 36 with with IN cord-310467-v2wlu5eq 93 37 80 80 CD cord-310467-v2wlu5eq 93 38 % % NN cord-310467-v2wlu5eq 93 39 acetone acetone NN cord-310467-v2wlu5eq 93 40 - - HYPH cord-310467-v2wlu5eq 93 41 fixed fix VBN cord-310467-v2wlu5eq 93 42 , , , cord-310467-v2wlu5eq 93 43 infected infected JJ cord-310467-v2wlu5eq 93 44 cells cell NNS cord-310467-v2wlu5eq 93 45 . . . cord-310467-v2wlu5eq 94 1 The the DT cord-310467-v2wlu5eq 94 2 degree degree NN cord-310467-v2wlu5eq 94 3 of of IN cord-310467-v2wlu5eq 94 4 antibody antibody NN cord-310467-v2wlu5eq 94 5 binding bind VBG cord-310467-v2wlu5eq 94 6 to to IN cord-310467-v2wlu5eq 94 7 the the DT cord-310467-v2wlu5eq 94 8 S S NNP cord-310467-v2wlu5eq 94 9 determinant determinant NN cord-310467-v2wlu5eq 94 10 in in IN cord-310467-v2wlu5eq 94 11 0.1 0.1 CD cord-310467-v2wlu5eq 94 12 % % NN cord-310467-v2wlu5eq 94 13 PFA PFA NNP cord-310467-v2wlu5eq 94 14 - - HYPH cord-310467-v2wlu5eq 94 15 fixed fix VBN cord-310467-v2wlu5eq 94 16 cells cell NNS cord-310467-v2wlu5eq 94 17 which which WDT cord-310467-v2wlu5eq 94 18 were be VBD cord-310467-v2wlu5eq 94 19 permeated permeate VBN cord-310467-v2wlu5eq 94 20 with with IN cord-310467-v2wlu5eq 94 21 different different JJ cord-310467-v2wlu5eq 94 22 concentrations concentration NNS cord-310467-v2wlu5eq 94 23 of of IN cord-310467-v2wlu5eq 94 24 Triton Triton NNP cord-310467-v2wlu5eq 94 25 X-100 x-100 NN cord-310467-v2wlu5eq 94 26 was be VBD cord-310467-v2wlu5eq 94 27 similar similar JJ cord-310467-v2wlu5eq 94 28 to to IN cord-310467-v2wlu5eq 95 1 that that DT cord-310467-v2wlu5eq 95 2 detected detect VBD cord-310467-v2wlu5eq 95 3 in in IN cord-310467-v2wlu5eq 95 4 80 80 CD cord-310467-v2wlu5eq 95 5 % % NN cord-310467-v2wlu5eq 95 6 acetone acetone NN cord-310467-v2wlu5eq 95 7 - - HYPH cord-310467-v2wlu5eq 95 8 fixed fix VBN cord-310467-v2wlu5eq 95 9 cells cell NNS cord-310467-v2wlu5eq 95 10 . . . cord-310467-v2wlu5eq 96 1 The the DT cord-310467-v2wlu5eq 96 2 OD od NN cord-310467-v2wlu5eq 96 3 of of IN cord-310467-v2wlu5eq 96 4 cells cell NNS cord-310467-v2wlu5eq 96 5 incubated incubate VBN cord-310467-v2wlu5eq 96 6 with with IN cord-310467-v2wlu5eq 96 7 anti anti JJ cord-310467-v2wlu5eq 96 8 - - JJ cord-310467-v2wlu5eq 96 9 M m JJ cord-310467-v2wlu5eq 96 10 MAb MAb NNS cord-310467-v2wlu5eq 96 11 was be VBD cord-310467-v2wlu5eq 96 12 equal equal JJ cord-310467-v2wlu5eq 96 13 to to IN cord-310467-v2wlu5eq 96 14 that that DT cord-310467-v2wlu5eq 96 15 obtained obtain VBN cord-310467-v2wlu5eq 96 16 from from IN cord-310467-v2wlu5eq 96 17 0.1 0.1 CD cord-310467-v2wlu5eq 96 18 % % NN cord-310467-v2wlu5eq 96 19 PFA PFA NNP cord-310467-v2wlu5eq 96 20 - - HYPH cord-310467-v2wlu5eq 96 21 fixed fix VBN cord-310467-v2wlu5eq 96 22 , , , cord-310467-v2wlu5eq 96 23 infected infected JJ cord-310467-v2wlu5eq 96 24 cells cell NNS cord-310467-v2wlu5eq 96 25 while while IN cord-310467-v2wlu5eq 96 26 the the DT cord-310467-v2wlu5eq 96 27 OD OD NNP cord-310467-v2wlu5eq 96 28 of of IN cord-310467-v2wlu5eq 96 29 cells cell NNS cord-310467-v2wlu5eq 96 30 incubated incubate VBN cord-310467-v2wlu5eq 96 31 with with IN cord-310467-v2wlu5eq 96 32 anti anti JJ cord-310467-v2wlu5eq 96 33 - - NNP cord-310467-v2wlu5eq 96 34 S S NNP cord-310467-v2wlu5eq 96 35 was be VBD cord-310467-v2wlu5eq 96 36 significantly significantly RB cord-310467-v2wlu5eq 96 37 lower low JJR cord-310467-v2wlu5eq 96 38 than than IN cord-310467-v2wlu5eq 96 39 that that DT cord-310467-v2wlu5eq 96 40 of of IN cord-310467-v2wlu5eq 96 41 0.1 0.1 CD cord-310467-v2wlu5eq 96 42 % % NN cord-310467-v2wlu5eq 96 43 PFA PFA NNP cord-310467-v2wlu5eq 96 44 - - HYPH cord-310467-v2wlu5eq 96 45 f'Lxed f'Lxed NNP cord-310467-v2wlu5eq 96 46 , , , cord-310467-v2wlu5eq 96 47 infected infected JJ cord-310467-v2wlu5eq 96 48 cells cell NNS cord-310467-v2wlu5eq 96 49 . . . cord-310467-v2wlu5eq 97 1 For for IN cord-310467-v2wlu5eq 97 2 some some DT cord-310467-v2wlu5eq 97 3 other other JJ cord-310467-v2wlu5eq 97 4 permeabilizing permeabilize VBG cord-310467-v2wlu5eq 97 5 agents agent NNS cord-310467-v2wlu5eq 97 6 ( ( -LRB- cord-310467-v2wlu5eq 97 7 Fig Fig NNP cord-310467-v2wlu5eq 97 8 . . . cord-310467-v2wlu5eq 97 9 6 6 CD cord-310467-v2wlu5eq 97 10 ) ) -RRB- cord-310467-v2wlu5eq 97 11 , , , cord-310467-v2wlu5eq 97 12 antibody antibody NN cord-310467-v2wlu5eq 97 13 binding bind VBG cord-310467-v2wlu5eq 97 14 to to IN cord-310467-v2wlu5eq 97 15 the the DT cord-310467-v2wlu5eq 97 16 N n CD cord-310467-v2wlu5eq 97 17 determinant determinant NN cord-310467-v2wlu5eq 97 18 in in IN cord-310467-v2wlu5eq 97 19 0.1 0.1 CD cord-310467-v2wlu5eq 97 20 % % NN cord-310467-v2wlu5eq 97 21 PFA PFA NNP cord-310467-v2wlu5eq 97 22 - - HYPH cord-310467-v2wlu5eq 97 23 fixed fix VBN cord-310467-v2wlu5eq 97 24 cells cell NNS cord-310467-v2wlu5eq 97 25 which which WDT cord-310467-v2wlu5eq 97 26 were be VBD cord-310467-v2wlu5eq 97 27 permeated permeate VBN cord-310467-v2wlu5eq 97 28 with with IN cord-310467-v2wlu5eq 97 29 either either DT cord-310467-v2wlu5eq 97 30 1 1 CD cord-310467-v2wlu5eq 97 31 % % NN cord-310467-v2wlu5eq 97 32 zwittergent zwittergent NN cord-310467-v2wlu5eq 97 33 , , , cord-310467-v2wlu5eq 97 34 1 1 CD cord-310467-v2wlu5eq 97 35 % % NN cord-310467-v2wlu5eq 97 36 empigen empigen NN cord-310467-v2wlu5eq 97 37 BB BB NNP cord-310467-v2wlu5eq 97 38 , , , cord-310467-v2wlu5eq 97 39 1 1 CD cord-310467-v2wlu5eq 97 40 % % NN cord-310467-v2wlu5eq 97 41 NP-40 NP-40 NNP cord-310467-v2wlu5eq 97 42 , , , cord-310467-v2wlu5eq 97 43 10 10 CD cord-310467-v2wlu5eq 97 44 mM mm CD cord-310467-v2wlu5eq 97 45 and and CC cord-310467-v2wlu5eq 97 46 50 50 CD cord-310467-v2wlu5eq 97 47 mM mm CD cord-310467-v2wlu5eq 98 1 Chaps chap NNS cord-310467-v2wlu5eq 98 2 showed show VBD cord-310467-v2wlu5eq 98 3 similarities similarity NNS cord-310467-v2wlu5eq 98 4 with with IN cord-310467-v2wlu5eq 98 5 the the DT cord-310467-v2wlu5eq 98 6 results result NNS cord-310467-v2wlu5eq 98 7 obtained obtain VBN cord-310467-v2wlu5eq 98 8 in in IN cord-310467-v2wlu5eq 98 9 80 80 CD cord-310467-v2wlu5eq 98 10 % % NN cord-310467-v2wlu5eq 98 11 acetone acetone NN cord-310467-v2wlu5eq 98 12 - - HYPH cord-310467-v2wlu5eq 98 13 fixed fix VBN cord-310467-v2wlu5eq 98 14 cells cell NNS cord-310467-v2wlu5eq 98 15 while while IN cord-310467-v2wlu5eq 98 16 antibody antibody NN cord-310467-v2wlu5eq 98 17 binding bind VBG cord-310467-v2wlu5eq 98 18 to to IN cord-310467-v2wlu5eq 98 19 this this DT cord-310467-v2wlu5eq 98 20 determinant determinant NN cord-310467-v2wlu5eq 98 21 in in IN cord-310467-v2wlu5eq 98 22 cells cell NNS cord-310467-v2wlu5eq 98 23 permeated permeate VBN cord-310467-v2wlu5eq 98 24 with with IN cord-310467-v2wlu5eq 98 25 10 10 CD cord-310467-v2wlu5eq 98 26 mM mm CD cord-310467-v2wlu5eq 98 27 N N NNP cord-310467-v2wlu5eq 98 28 - - HYPH cord-310467-v2wlu5eq 98 29 lauroyl lauroyl NNP cord-310467-v2wlu5eq 98 30 and and CC cord-310467-v2wlu5eq 98 31 1 1 CD cord-310467-v2wlu5eq 98 32 mM mm CD cord-310467-v2wlu5eq 98 33 Chaps Chaps NNPS cord-310467-v2wlu5eq 98 34 was be VBD cord-310467-v2wlu5eq 98 35 lower low JJR cord-310467-v2wlu5eq 98 36 than than IN cord-310467-v2wlu5eq 98 37 that that DT cord-310467-v2wlu5eq 98 38 of of IN cord-310467-v2wlu5eq 98 39 80 80 CD cord-310467-v2wlu5eq 98 40 % % NN cord-310467-v2wlu5eq 98 41 acetonefixed acetonefixed NN cord-310467-v2wlu5eq 98 42 , , , cord-310467-v2wlu5eq 98 43 infected infected JJ cord-310467-v2wlu5eq 98 44 cells cell NNS cord-310467-v2wlu5eq 98 45 . . . cord-310467-v2wlu5eq 99 1 The the DT cord-310467-v2wlu5eq 99 2 degree degree NN cord-310467-v2wlu5eq 99 3 of of IN cord-310467-v2wlu5eq 99 4 antibody antibody NN cord-310467-v2wlu5eq 99 5 binding bind VBG cord-310467-v2wlu5eq 99 6 to to IN cord-310467-v2wlu5eq 99 7 the the DT cord-310467-v2wlu5eq 99 8 S S NNP cord-310467-v2wlu5eq 99 9 and and CC cord-310467-v2wlu5eq 99 10 M M NNP cord-310467-v2wlu5eq 99 11 determinants determinant NNS cord-310467-v2wlu5eq 99 12 in in IN cord-310467-v2wlu5eq 99 13 cells cell NNS cord-310467-v2wlu5eq 99 14 which which WDT cord-310467-v2wlu5eq 99 15 were be VBD cord-310467-v2wlu5eq 99 16 permeated permeate VBN cord-310467-v2wlu5eq 99 17 with with IN cord-310467-v2wlu5eq 99 18 1 1 CD cord-310467-v2wlu5eq 99 19 % % NN cord-310467-v2wlu5eq 99 20 zwittergent zwittergent NN cord-310467-v2wlu5eq 99 21 3 3 CD cord-310467-v2wlu5eq 99 22 - - SYM cord-310467-v2wlu5eq 99 23 12 12 CD cord-310467-v2wlu5eq 99 24 or or CC cord-310467-v2wlu5eq 99 25 10 10 CD cord-310467-v2wlu5eq 100 1 mM mM NNP cord-310467-v2wlu5eq 100 2 N N NNP cord-310467-v2wlu5eq 100 3 - - HYPH cord-310467-v2wlu5eq 100 4 lauroyl lauroyl NNP cord-310467-v2wlu5eq 100 5 was be VBD cord-310467-v2wlu5eq 100 6 lower low JJR cord-310467-v2wlu5eq 100 7 than than IN cord-310467-v2wlu5eq 100 8 that that DT cord-310467-v2wlu5eq 100 9 of of IN cord-310467-v2wlu5eq 100 10 80 80 CD cord-310467-v2wlu5eq 100 11 % % NN cord-310467-v2wlu5eq 100 12 acetone acetone NN cord-310467-v2wlu5eq 100 13 - - HYPH cord-310467-v2wlu5eq 100 14 luted lute VBN cord-310467-v2wlu5eq 100 15 , , , cord-310467-v2wlu5eq 100 16 infected infected JJ cord-310467-v2wlu5eq 100 17 cells cell NNS cord-310467-v2wlu5eq 100 18 . . . cord-310467-v2wlu5eq 101 1 The the DT cord-310467-v2wlu5eq 101 2 degree degree NN cord-310467-v2wlu5eq 101 3 of of IN cord-310467-v2wlu5eq 101 4 antibody antibody NN cord-310467-v2wlu5eq 101 5 binding bind VBG cord-310467-v2wlu5eq 101 6 to to IN cord-310467-v2wlu5eq 101 7 these these DT cord-310467-v2wlu5eq 101 8 determinants determinant NNS cord-310467-v2wlu5eq 101 9 in in IN cord-310467-v2wlu5eq 101 10 cells cell NNS cord-310467-v2wlu5eq 101 11 permeated permeate VBN cord-310467-v2wlu5eq 101 12 with with IN cord-310467-v2wlu5eq 101 13 1 1 CD cord-310467-v2wlu5eq 101 14 % % NN cord-310467-v2wlu5eq 101 15 zwittergent zwittergent NN cord-310467-v2wlu5eq 101 16 3 3 CD cord-310467-v2wlu5eq 101 17 - - SYM cord-310467-v2wlu5eq 101 18 16 16 CD cord-310467-v2wlu5eq 101 19 , , , cord-310467-v2wlu5eq 101 20 or or CC cord-310467-v2wlu5eq 101 21 1 1 CD cord-310467-v2wlu5eq 101 22 % % NN cord-310467-v2wlu5eq 101 23 empigen empigen NN cord-310467-v2wlu5eq 101 24 BB BB NNP cord-310467-v2wlu5eq 101 25 , , , cord-310467-v2wlu5eq 101 26 1 1 CD cord-310467-v2wlu5eq 101 27 % % NN cord-310467-v2wlu5eq 102 1 NP-40 NP-40 NNP cord-310467-v2wlu5eq 102 2 or or CC cord-310467-v2wlu5eq 102 3 Chaps Chaps NNPS cord-310467-v2wlu5eq 102 4 was be VBD cord-310467-v2wlu5eq 102 5 similar similar JJ cord-310467-v2wlu5eq 102 6 to to IN cord-310467-v2wlu5eq 102 7 that that DT cord-310467-v2wlu5eq 102 8 obtained obtain VBN cord-310467-v2wlu5eq 102 9 in in IN cord-310467-v2wlu5eq 102 10 80 80 CD cord-310467-v2wlu5eq 102 11 % % NN cord-310467-v2wlu5eq 102 12 acetone acetone NN cord-310467-v2wlu5eq 102 13 - - HYPH cord-310467-v2wlu5eq 102 14 fixed fix VBN cord-310467-v2wlu5eq 102 15 , , , cord-310467-v2wlu5eq 102 16 infected infected JJ cord-310467-v2wlu5eq 102 17 cells cell NNS cord-310467-v2wlu5eq 102 18 . . . cord-310467-v2wlu5eq 103 1 The the DT cord-310467-v2wlu5eq 103 2 following follow VBG cord-310467-v2wlu5eq 103 3 experiments experiment NNS cord-310467-v2wlu5eq 103 4 were be VBD cord-310467-v2wlu5eq 103 5 carried carry VBN cord-310467-v2wlu5eq 103 6 out out RP cord-310467-v2wlu5eq 103 7 to to TO cord-310467-v2wlu5eq 103 8 identify identify VB cord-310467-v2wlu5eq 103 9 differences difference NNS cord-310467-v2wlu5eq 103 10 in in IN cord-310467-v2wlu5eq 103 11 localisation localisation NN cord-310467-v2wlu5eq 103 12 of of IN cord-310467-v2wlu5eq 103 13 antigens antigen NNS cord-310467-v2wlu5eq 103 14 detected detect VBN cord-310467-v2wlu5eq 103 15 in in IN cord-310467-v2wlu5eq 103 16 infected infected JJ cord-310467-v2wlu5eq 103 17 cells cell NNS cord-310467-v2wlu5eq 103 18 which which WDT cord-310467-v2wlu5eq 103 19 were be VBD cord-310467-v2wlu5eq 103 20 fixed fix VBN cord-310467-v2wlu5eq 103 21 with with IN cord-310467-v2wlu5eq 103 22 either either DT cord-310467-v2wlu5eq 103 23 0.1 0.1 CD cord-310467-v2wlu5eq 103 24 % % NN cord-310467-v2wlu5eq 103 25 PFA PFA NNP cord-310467-v2wlu5eq 103 26 at at IN cord-310467-v2wlu5eq 103 27 4 4 CD cord-310467-v2wlu5eq 103 28 ° ° NN cord-310467-v2wlu5eq 103 29 C c NN cord-310467-v2wlu5eq 103 30 ( ( -LRB- cord-310467-v2wlu5eq 103 31 Fig Fig NNP cord-310467-v2wlu5eq 103 32 . . . cord-310467-v2wlu5eq 103 33 7a 7a NN cord-310467-v2wlu5eq 103 34 ) ) -RRB- cord-310467-v2wlu5eq 103 35 or or CC cord-310467-v2wlu5eq 103 36 with with IN cord-310467-v2wlu5eq 103 37 80 80 CD cord-310467-v2wlu5eq 103 38 % % NN cord-310467-v2wlu5eq 103 39 acetone acetone NN cord-310467-v2wlu5eq 103 40 at at IN cord-310467-v2wlu5eq 103 41 -20 -20 NNP cord-310467-v2wlu5eq 103 42 ° ° NNP cord-310467-v2wlu5eq 103 43 C C NNP cord-310467-v2wlu5eq 103 44 ( ( -LRB- cord-310467-v2wlu5eq 103 45 Fig Fig NNP cord-310467-v2wlu5eq 103 46 . . . cord-310467-v2wlu5eq 103 47 7b 7b CD cord-310467-v2wlu5eq 103 48 ) ) -RRB- cord-310467-v2wlu5eq 103 49 using use VBG cord-310467-v2wlu5eq 103 50 two two CD cord-310467-v2wlu5eq 103 51 different different JJ cord-310467-v2wlu5eq 103 52 fixation fixation NN cord-310467-v2wlu5eq 103 53 procedures procedure NNS cord-310467-v2wlu5eq 103 54 . . . cord-310467-v2wlu5eq 104 1 The the DT cord-310467-v2wlu5eq 104 2 degree degree NN cord-310467-v2wlu5eq 104 3 of of IN cord-310467-v2wlu5eq 104 4 antibody antibody NN cord-310467-v2wlu5eq 104 5 binding bind VBG cord-310467-v2wlu5eq 104 6 to to IN cord-310467-v2wlu5eq 104 7 viral viral JJ cord-310467-v2wlu5eq 104 8 determinants determinant NNS cord-310467-v2wlu5eq 104 9 in in IN cord-310467-v2wlu5eq 104 10 unfixed unfixed JJ cord-310467-v2wlu5eq 104 11 , , , cord-310467-v2wlu5eq 104 12 infected infected JJ cord-310467-v2wlu5eq 104 13 control control NN cord-310467-v2wlu5eq 104 14 cells cell NNS cord-310467-v2wlu5eq 104 15 was be VBD cord-310467-v2wlu5eq 104 16 compared compare VBN cord-310467-v2wlu5eq 104 17 with with IN cord-310467-v2wlu5eq 104 18 that that DT cord-310467-v2wlu5eq 104 19 measured measure VBN cord-310467-v2wlu5eq 104 20 in in IN cord-310467-v2wlu5eq 104 21 infected infected JJ cord-310467-v2wlu5eq 104 22 cells cell NNS cord-310467-v2wlu5eq 104 23 which which WDT cord-310467-v2wlu5eq 104 24 were be VBD cord-310467-v2wlu5eq 104 25 fixed fix VBN cord-310467-v2wlu5eq 104 26 ( ( -LRB- cord-310467-v2wlu5eq 104 27 i i NN cord-310467-v2wlu5eq 104 28 ) ) -RRB- cord-310467-v2wlu5eq 104 29 before before IN cord-310467-v2wlu5eq 104 30 their -PRON- PRP$ cord-310467-v2wlu5eq 104 31 incubation incubation NN cord-310467-v2wlu5eq 104 32 with with IN cord-310467-v2wlu5eq 104 33 antibody antibody NN cord-310467-v2wlu5eq 104 34 or or CC cord-310467-v2wlu5eq 104 35 ( ( -LRB- cord-310467-v2wlu5eq 104 36 ii ii NN cord-310467-v2wlu5eq 104 37 ) ) -RRB- cord-310467-v2wlu5eq 104 38 after after IN cord-310467-v2wlu5eq 104 39 their -PRON- PRP$ cord-310467-v2wlu5eq 104 40 incubation incubation NN cord-310467-v2wlu5eq 104 41 with with IN cord-310467-v2wlu5eq 104 42 antibody antibody NN cord-310467-v2wlu5eq 104 43 . . . cord-310467-v2wlu5eq 105 1 The the DT cord-310467-v2wlu5eq 105 2 antibodies antibody NNS cord-310467-v2wlu5eq 105 3 used use VBN cord-310467-v2wlu5eq 105 4 in in IN cord-310467-v2wlu5eq 105 5 this this DT cord-310467-v2wlu5eq 105 6 experiment experiment NN cord-310467-v2wlu5eq 105 7 were be VBD cord-310467-v2wlu5eq 105 8 MAbs MAbs NNP cord-310467-v2wlu5eq 105 9 and and CC cord-310467-v2wlu5eq 105 10 PAbs PAbs NNP cord-310467-v2wlu5eq 105 11 as as IN cord-310467-v2wlu5eq 105 12 mentioned mention VBN cord-310467-v2wlu5eq 105 13 in in IN cord-310467-v2wlu5eq 105 14 the the DT cord-310467-v2wlu5eq 105 15 materials material NNS cord-310467-v2wlu5eq 105 16 and and CC cord-310467-v2wlu5eq 105 17 methods method NNS cord-310467-v2wlu5eq 105 18 section section NN cord-310467-v2wlu5eq 105 19 . . . cord-310467-v2wlu5eq 106 1 Whichever whichever WDT cord-310467-v2wlu5eq 106 2 PFA PFA NNP cord-310467-v2wlu5eq 106 3 fixation fixation NN cord-310467-v2wlu5eq 106 4 procedure procedure NN cord-310467-v2wlu5eq 106 5 was be VBD cord-310467-v2wlu5eq 106 6 used use VBN cord-310467-v2wlu5eq 106 7 , , , cord-310467-v2wlu5eq 106 8 the the DT cord-310467-v2wlu5eq 106 9 degree degree NN cord-310467-v2wlu5eq 106 10 of of IN cord-310467-v2wlu5eq 106 11 antibody antibody NN cord-310467-v2wlu5eq 106 12 binding bind VBG cord-310467-v2wlu5eq 106 13 to to IN cord-310467-v2wlu5eq 106 14 the the DT cord-310467-v2wlu5eq 106 15 M M NNP cord-310467-v2wlu5eq 106 16 , , , cord-310467-v2wlu5eq 106 17 S S NNP cord-310467-v2wlu5eq 106 18 , , , cord-310467-v2wlu5eq 106 19 N n CD cord-310467-v2wlu5eq 106 20 determinants determinant NNS cord-310467-v2wlu5eq 106 21 and and CC cord-310467-v2wlu5eq 106 22 to to IN cord-310467-v2wlu5eq 106 23 virions virion NNS cord-310467-v2wlu5eq 106 24 in in IN cord-310467-v2wlu5eq 106 25 TOEV TOEV NNP cord-310467-v2wlu5eq 106 26 - - HYPH cord-310467-v2wlu5eq 106 27 infected infect VBN cord-310467-v2wlu5eq 106 28 cells cell NNS cord-310467-v2wlu5eq 106 29 was be VBD cord-310467-v2wlu5eq 106 30 not not RB cord-310467-v2wlu5eq 106 31 significantly significantly RB cord-310467-v2wlu5eq 106 32 different different JJ cord-310467-v2wlu5eq 106 33 from from IN cord-310467-v2wlu5eq 106 34 that that DT cord-310467-v2wlu5eq 106 35 of of IN cord-310467-v2wlu5eq 106 36 controls control NNS cord-310467-v2wlu5eq 106 37 . . . cord-310467-v2wlu5eq 107 1 However however RB cord-310467-v2wlu5eq 107 2 , , , cord-310467-v2wlu5eq 107 3 antibody antibody NN cord-310467-v2wlu5eq 107 4 binding bind VBG cord-310467-v2wlu5eq 107 5 to to IN cord-310467-v2wlu5eq 107 6 viral viral JJ cord-310467-v2wlu5eq 107 7 determinants determinant NNS cord-310467-v2wlu5eq 107 8 varied vary VBN cord-310467-v2wlu5eq 107 9 between between IN cord-310467-v2wlu5eq 107 10 cells cell NNS cord-310467-v2wlu5eq 107 11 fixed fix VBN cord-310467-v2wlu5eq 107 12 with with IN cord-310467-v2wlu5eq 107 13 80 80 CD cord-310467-v2wlu5eq 107 14 % % NN cord-310467-v2wlu5eq 107 15 acetone acetone NN cord-310467-v2wlu5eq 107 16 at at IN cord-310467-v2wlu5eq 107 17 -20 -20 NNP cord-310467-v2wlu5eq 107 18 ° ° NNP cord-310467-v2wlu5eq 107 19 C C NNP cord-310467-v2wlu5eq 107 20 and and CC cord-310467-v2wlu5eq 107 21 the the DT cord-310467-v2wlu5eq 107 22 control control NN cord-310467-v2wlu5eq 107 23 infected infected JJ cord-310467-v2wlu5eq 107 24 cells cell NNS cord-310467-v2wlu5eq 107 25 . . . cord-310467-v2wlu5eq 108 1 The the DT cord-310467-v2wlu5eq 108 2 degree degree NN cord-310467-v2wlu5eq 108 3 of of IN cord-310467-v2wlu5eq 108 4 antibody antibody NN cord-310467-v2wlu5eq 108 5 binding bind VBG cord-310467-v2wlu5eq 108 6 to to IN cord-310467-v2wlu5eq 108 7 the the DT cord-310467-v2wlu5eq 108 8 M M NNP cord-310467-v2wlu5eq 108 9 determinant determinant NN cord-310467-v2wlu5eq 108 10 in in IN cord-310467-v2wlu5eq 108 11 cells cell NNS cord-310467-v2wlu5eq 108 12 fred fred NNP cord-310467-v2wlu5eq 108 13 according accord VBG cord-310467-v2wlu5eq 108 14 to to IN cord-310467-v2wlu5eq 108 15 the the DT cord-310467-v2wlu5eq 108 16 two two CD cord-310467-v2wlu5eq 108 17 different different JJ cord-310467-v2wlu5eq 108 18 fixation fixation NN cord-310467-v2wlu5eq 108 19 procedures procedure NNS cord-310467-v2wlu5eq 108 20 was be VBD cord-310467-v2wlu5eq 108 21 similar similar JJ cord-310467-v2wlu5eq 108 22 to to IN cord-310467-v2wlu5eq 108 23 that that DT cord-310467-v2wlu5eq 108 24 obtained obtain VBN cord-310467-v2wlu5eq 108 25 in in IN cord-310467-v2wlu5eq 108 26 the the DT cord-310467-v2wlu5eq 108 27 controls control NNS cord-310467-v2wlu5eq 108 28 . . . cord-310467-v2wlu5eq 109 1 The the DT cord-310467-v2wlu5eq 109 2 degree degree NN cord-310467-v2wlu5eq 109 3 of of IN cord-310467-v2wlu5eq 109 4 antibody antibody NN cord-310467-v2wlu5eq 109 5 binding bind VBG cord-310467-v2wlu5eq 109 6 to to IN cord-310467-v2wlu5eq 109 7 the the DT cord-310467-v2wlu5eq 109 8 S S NNP cord-310467-v2wlu5eq 109 9 determinant determinant NN cord-310467-v2wlu5eq 109 10 was be VBD cord-310467-v2wlu5eq 109 11 lower low JJR cord-310467-v2wlu5eq 109 12 than than IN cord-310467-v2wlu5eq 109 13 that that DT cord-310467-v2wlu5eq 109 14 with with IN cord-310467-v2wlu5eq 109 15 the the DT cord-310467-v2wlu5eq 109 16 controls control NNS cord-310467-v2wlu5eq 109 17 suggesting suggest VBG cord-310467-v2wlu5eq 109 18 that that IN cord-310467-v2wlu5eq 109 19 after after IN cord-310467-v2wlu5eq 109 20 acetone acetone NN cord-310467-v2wlu5eq 109 21 fixation fixation NN cord-310467-v2wlu5eq 109 22 the the DT cord-310467-v2wlu5eq 109 23 accessibility accessibility NN cord-310467-v2wlu5eq 109 24 of of IN cord-310467-v2wlu5eq 109 25 this this DT cord-310467-v2wlu5eq 109 26 determinant determinant NN cord-310467-v2wlu5eq 109 27 to to IN cord-310467-v2wlu5eq 109 28 the the DT cord-310467-v2wlu5eq 109 29 MAb MAb NNS cord-310467-v2wlu5eq 109 30 was be VBD cord-310467-v2wlu5eq 109 31 affected affect VBN cord-310467-v2wlu5eq 109 32 . . . cord-310467-v2wlu5eq 110 1 The the DT cord-310467-v2wlu5eq 110 2 degree degree NN cord-310467-v2wlu5eq 110 3 of of IN cord-310467-v2wlu5eq 110 4 antibody antibody NN cord-310467-v2wlu5eq 110 5 binding bind VBG cord-310467-v2wlu5eq 110 6 to to IN cord-310467-v2wlu5eq 110 7 the the DT cord-310467-v2wlu5eq 110 8 N n CD cord-310467-v2wlu5eq 110 9 determinant determinant NN cord-310467-v2wlu5eq 110 10 in in IN cord-310467-v2wlu5eq 110 11 cells cell NNS cord-310467-v2wlu5eq 110 12 fixed fix VBN cord-310467-v2wlu5eq 110 13 with with IN cord-310467-v2wlu5eq 110 14 80 80 CD cord-310467-v2wlu5eq 110 15 % % NN cord-310467-v2wlu5eq 110 16 acetone acetone NN cord-310467-v2wlu5eq 110 17 after after IN cord-310467-v2wlu5eq 110 18 their -PRON- PRP$ cord-310467-v2wlu5eq 110 19 incubation incubation NN cord-310467-v2wlu5eq 110 20 with with IN cord-310467-v2wlu5eq 110 21 MAb MAb NNS cord-310467-v2wlu5eq 110 22 was be VBD cord-310467-v2wlu5eq 110 23 equal equal JJ cord-310467-v2wlu5eq 110 24 to to IN cord-310467-v2wlu5eq 110 25 that that DT cord-310467-v2wlu5eq 110 26 of of IN cord-310467-v2wlu5eq 110 27 controls control NNS cord-310467-v2wlu5eq 110 28 but but CC cord-310467-v2wlu5eq 110 29 considerably considerably RB cord-310467-v2wlu5eq 110 30 lower low JJR cord-310467-v2wlu5eq 110 31 than than IN cord-310467-v2wlu5eq 110 32 that that DT cord-310467-v2wlu5eq 110 33 of of IN cord-310467-v2wlu5eq 110 34 infected infected JJ cord-310467-v2wlu5eq 110 35 cells cell NNS cord-310467-v2wlu5eq 110 36 which which WDT cord-310467-v2wlu5eq 110 37 were be VBD cord-310467-v2wlu5eq 110 38 fixed fix VBN cord-310467-v2wlu5eq 110 39 with with IN cord-310467-v2wlu5eq 110 40 80 80 CD cord-310467-v2wlu5eq 110 41 % % NN cord-310467-v2wlu5eq 110 42 acetone acetone NN cord-310467-v2wlu5eq 110 43 prior prior RB cord-310467-v2wlu5eq 110 44 to to IN cord-310467-v2wlu5eq 110 45 their -PRON- PRP$ cord-310467-v2wlu5eq 110 46 incubation incubation NN cord-310467-v2wlu5eq 110 47 with with IN cord-310467-v2wlu5eq 110 48 MAb MAb NNS cord-310467-v2wlu5eq 110 49 . . . cord-310467-v2wlu5eq 111 1 The the DT cord-310467-v2wlu5eq 111 2 anti anti JJ cord-310467-v2wlu5eq 111 3 - - JJ cord-310467-v2wlu5eq 111 4 TGEV tgev JJ cord-310467-v2wlu5eq 111 5 polyclonal polyclonal JJ cord-310467-v2wlu5eq 111 6 binding binding NN cord-310467-v2wlu5eq 111 7 profiles profile NNS cord-310467-v2wlu5eq 111 8 to to IN cord-310467-v2wlu5eq 111 9 viral viral JJ cord-310467-v2wlu5eq 111 10 antigens antigen NNS cord-310467-v2wlu5eq 111 11 were be VBD cord-310467-v2wlu5eq 111 12 similar similar JJ cord-310467-v2wlu5eq 111 13 to to IN cord-310467-v2wlu5eq 111 14 those those DT cord-310467-v2wlu5eq 111 15 obtained obtain VBN cord-310467-v2wlu5eq 111 16 with with IN cord-310467-v2wlu5eq 111 17 the the DT cord-310467-v2wlu5eq 111 18 M M NNP cord-310467-v2wlu5eq 111 19 and and CC cord-310467-v2wlu5eq 111 20 S S NNP cord-310467-v2wlu5eq 111 21 determinants determinant NNS cord-310467-v2wlu5eq 111 22 . . . cord-310467-v2wlu5eq 112 1 The the DT cord-310467-v2wlu5eq 112 2 present present JJ cord-310467-v2wlu5eq 112 3 study study NN cord-310467-v2wlu5eq 112 4 has have VBZ cord-310467-v2wlu5eq 112 5 shown show VBN cord-310467-v2wlu5eq 112 6 that that IN cord-310467-v2wlu5eq 112 7 GA GA NNP cord-310467-v2wlu5eq 112 8 fixation fixation NN cord-310467-v2wlu5eq 112 9 alters alter VBZ cord-310467-v2wlu5eq 112 10 the the DT cord-310467-v2wlu5eq 112 11 accessibility accessibility NN cord-310467-v2wlu5eq 112 12 of of IN cord-310467-v2wlu5eq 112 13 TGEV TGEV NNP cord-310467-v2wlu5eq 112 14 determinants determinant NNS cord-310467-v2wlu5eq 112 15 to to IN cord-310467-v2wlu5eq 112 16 anti anti JJ cord-310467-v2wlu5eq 112 17 - - NNP cord-310467-v2wlu5eq 112 18 M m JJ cord-310467-v2wlu5eq 112 19 , , , cord-310467-v2wlu5eq 112 20 anti anti NNP cord-310467-v2wlu5eq 112 21 - - NNP cord-310467-v2wlu5eq 112 22 S s JJ cord-310467-v2wlu5eq 112 23 and and CC cord-310467-v2wlu5eq 112 24 anti anti JJ cord-310467-v2wlu5eq 112 25 - - NNP cord-310467-v2wlu5eq 112 26 N N NNP cord-310467-v2wlu5eq 112 27 MAbs MAbs NNP cord-310467-v2wlu5eq 112 28 and and CC cord-310467-v2wlu5eq 112 29 that that IN cord-310467-v2wlu5eq 112 30 FA fa NN cord-310467-v2wlu5eq 112 31 fixation fixation NN cord-310467-v2wlu5eq 112 32 has have VBZ cord-310467-v2wlu5eq 112 33 the the DT cord-310467-v2wlu5eq 112 34 opposite opposite JJ cord-310467-v2wlu5eq 112 35 effect effect NN cord-310467-v2wlu5eq 112 36 on on IN cord-310467-v2wlu5eq 112 37 the the DT cord-310467-v2wlu5eq 112 38 detection detection NN cord-310467-v2wlu5eq 112 39 of of IN cord-310467-v2wlu5eq 112 40 M M NNP cord-310467-v2wlu5eq 112 41 and and CC cord-310467-v2wlu5eq 112 42 S S NNP cord-310467-v2wlu5eq 112 43 viral viral JJ cord-310467-v2wlu5eq 112 44 determinants determinant NNS cord-310467-v2wlu5eq 112 45 compared compare VBN cord-310467-v2wlu5eq 112 46 with with IN cord-310467-v2wlu5eq 112 47 PFA PFA NNP cord-310467-v2wlu5eq 112 48 fixation fixation NN cord-310467-v2wlu5eq 112 49 . . . cord-310467-v2wlu5eq 113 1 In in IN cord-310467-v2wlu5eq 113 2 another another DT cord-310467-v2wlu5eq 113 3 study study NN cord-310467-v2wlu5eq 113 4 Smith Smith NNP cord-310467-v2wlu5eq 113 5 et et NNP cord-310467-v2wlu5eq 113 6 al al NNP cord-310467-v2wlu5eq 113 7 . . . cord-310467-v2wlu5eq 114 1 ( ( -LRB- cord-310467-v2wlu5eq 114 2 1988 1988 CD cord-310467-v2wlu5eq 114 3 ) ) -RRB- cord-310467-v2wlu5eq 114 4 has have VBZ cord-310467-v2wlu5eq 114 5 shown show VBN cord-310467-v2wlu5eq 114 6 that that IN cord-310467-v2wlu5eq 114 7 formalin formalin NN cord-310467-v2wlu5eq 114 8 fixation fixation NN cord-310467-v2wlu5eq 114 9 blocks block NNS cord-310467-v2wlu5eq 114 10 , , , cord-310467-v2wlu5eq 114 11 alters alter VBZ cord-310467-v2wlu5eq 114 12 or or CC cord-310467-v2wlu5eq 114 13 destroys destroy VBZ cord-310467-v2wlu5eq 114 14 a a DT cord-310467-v2wlu5eq 114 15 specific specific JJ cord-310467-v2wlu5eq 114 16 antigen antigen NN cord-310467-v2wlu5eq 114 17 expressed express VBN cord-310467-v2wlu5eq 114 18 in in IN cord-310467-v2wlu5eq 114 19 bovine bovine JJ cord-310467-v2wlu5eq 114 20 viral viral JJ cord-310467-v2wlu5eq 114 21 disease disease NN cord-310467-v2wlu5eq 114 22 virus virus NN cord-310467-v2wlu5eq 114 23 ( ( -LRB- cord-310467-v2wlu5eq 114 24 BVDV)-infected bvdv)-infected CD cord-310467-v2wlu5eq 114 25 cells cell NNS cord-310467-v2wlu5eq 114 26 . . . cord-310467-v2wlu5eq 115 1 Similar similar JJ cord-310467-v2wlu5eq 115 2 results result NNS cord-310467-v2wlu5eq 115 3 were be VBD cord-310467-v2wlu5eq 115 4 also also RB cord-310467-v2wlu5eq 115 5 obtained obtain VBN cord-310467-v2wlu5eq 115 6 by by IN cord-310467-v2wlu5eq 115 7 Saunders Saunders NNP cord-310467-v2wlu5eq 115 8 ( ( -LRB- cord-310467-v2wlu5eq 115 9 1977 1977 CD cord-310467-v2wlu5eq 115 10 ) ) -RRB- cord-310467-v2wlu5eq 115 11 for for IN cord-310467-v2wlu5eq 115 12 the the DT cord-310467-v2wlu5eq 115 13 detection detection NN cord-310467-v2wlu5eq 115 14 of of IN cord-310467-v2wlu5eq 115 15 hog hog NN cord-310467-v2wlu5eq 115 16 cholera cholera NN cord-310467-v2wlu5eq 115 17 virus virus NN cord-310467-v2wlu5eq 115 18 antigen antigen NN cord-310467-v2wlu5eq 115 19 after after IN cord-310467-v2wlu5eq 115 20 GA GA NNP cord-310467-v2wlu5eq 115 21 f f NNP cord-310467-v2wlu5eq 115 22 ~ ~ NFP cord-310467-v2wlu5eq 115 23 ation ation NN cord-310467-v2wlu5eq 115 24 at at IN cord-310467-v2wlu5eq 115 25 different different JJ cord-310467-v2wlu5eq 115 26 concentrations concentration NNS cord-310467-v2wlu5eq 115 27 . . . cord-310467-v2wlu5eq 116 1 To to TO cord-310467-v2wlu5eq 116 2 investigate investigate VB cord-310467-v2wlu5eq 116 3 the the DT cord-310467-v2wlu5eq 116 4 influence influence NN cord-310467-v2wlu5eq 116 5 of of IN cord-310467-v2wlu5eq 116 6 fixation fixation NN cord-310467-v2wlu5eq 116 7 temperature temperature NN cord-310467-v2wlu5eq 116 8 on on IN cord-310467-v2wlu5eq 116 9 viral viral JJ cord-310467-v2wlu5eq 116 10 antigen antigen NN cord-310467-v2wlu5eq 116 11 detection detection NN cord-310467-v2wlu5eq 116 12 , , , cord-310467-v2wlu5eq 116 13 the the DT cord-310467-v2wlu5eq 116 14 fixation fixation NN cord-310467-v2wlu5eq 116 15 step step NN cord-310467-v2wlu5eq 116 16 was be VBD cord-310467-v2wlu5eq 116 17 carried carry VBN cord-310467-v2wlu5eq 116 18 out out RP cord-310467-v2wlu5eq 116 19 at at IN cord-310467-v2wlu5eq 116 20 -20 -20 NNP cord-310467-v2wlu5eq 116 21 ° ° NNP cord-310467-v2wlu5eq 116 22 C C NNP cord-310467-v2wlu5eq 116 23 , , , cord-310467-v2wlu5eq 116 24 4 4 CD cord-310467-v2wlu5eq 116 25 ° ° NN cord-310467-v2wlu5eq 116 26 C c NN cord-310467-v2wlu5eq 116 27 or or CC cord-310467-v2wlu5eq 116 28 at at IN cord-310467-v2wlu5eq 116 29 RT RT NNP cord-310467-v2wlu5eq 116 30 . . . cord-310467-v2wlu5eq 117 1 The the DT cord-310467-v2wlu5eq 117 2 detectable detectable JJ cord-310467-v2wlu5eq 117 3 quantity quantity NN cord-310467-v2wlu5eq 117 4 of of IN cord-310467-v2wlu5eq 117 5 M M NNP cord-310467-v2wlu5eq 117 6 antigen antigen NN cord-310467-v2wlu5eq 117 7 decreased decrease VBD cord-310467-v2wlu5eq 117 8 with with IN cord-310467-v2wlu5eq 117 9 an an DT cord-310467-v2wlu5eq 117 10 increase increase NN cord-310467-v2wlu5eq 117 11 in in IN cord-310467-v2wlu5eq 117 12 acetone acetone NN cord-310467-v2wlu5eq 117 13 concentration concentration NN cord-310467-v2wlu5eq 117 14 at at IN cord-310467-v2wlu5eq 117 15 all all DT cord-310467-v2wlu5eq 117 16 three three CD cord-310467-v2wlu5eq 117 17 test test NN cord-310467-v2wlu5eq 117 18 temperatures temperature NNS cord-310467-v2wlu5eq 117 19 . . . cord-310467-v2wlu5eq 118 1 The the DT cord-310467-v2wlu5eq 118 2 increase increase NN cord-310467-v2wlu5eq 118 3 in in IN cord-310467-v2wlu5eq 118 4 acetone acetone NN cord-310467-v2wlu5eq 118 5 concentration concentration NN cord-310467-v2wlu5eq 118 6 parallelled parallel VBD cord-310467-v2wlu5eq 118 7 the the DT cord-310467-v2wlu5eq 118 8 increase increase NN cord-310467-v2wlu5eq 118 9 of of IN cord-310467-v2wlu5eq 118 10 detectable detectable JJ cord-310467-v2wlu5eq 118 11 N n CD cord-310467-v2wlu5eq 118 12 antigen antigen NN cord-310467-v2wlu5eq 118 13 . . . cord-310467-v2wlu5eq 119 1 Our -PRON- PRP$ cord-310467-v2wlu5eq 119 2 results result NNS cord-310467-v2wlu5eq 119 3 suggest suggest VBP cord-310467-v2wlu5eq 119 4 that that IN cord-310467-v2wlu5eq 119 5 the the DT cord-310467-v2wlu5eq 119 6 acetone acetone NN cord-310467-v2wlu5eq 119 7 concentration concentration NN cord-310467-v2wlu5eq 119 8 in in IN cord-310467-v2wlu5eq 119 9 fixative fixative NN cord-310467-v2wlu5eq 119 10 preparation preparation NN cord-310467-v2wlu5eq 119 11 and and CC cord-310467-v2wlu5eq 119 12 the the DT cord-310467-v2wlu5eq 119 13 fixa fixa NNP cord-310467-v2wlu5eq 119 14 - - HYPH cord-310467-v2wlu5eq 119 15 tion tion NN cord-310467-v2wlu5eq 119 16 temperature temperature NN cord-310467-v2wlu5eq 119 17 are be VBP cord-310467-v2wlu5eq 119 18 two two CD cord-310467-v2wlu5eq 119 19 important important JJ cord-310467-v2wlu5eq 119 20 criteria criterion NNS cord-310467-v2wlu5eq 119 21 for for IN cord-310467-v2wlu5eq 119 22 a a DT cord-310467-v2wlu5eq 119 23 fixation fixation NN cord-310467-v2wlu5eq 119 24 procedure procedure NN cord-310467-v2wlu5eq 119 25 . . . cord-310467-v2wlu5eq 120 1 Similar similar JJ cord-310467-v2wlu5eq 120 2 results result NNS cord-310467-v2wlu5eq 120 3 were be VBD cord-310467-v2wlu5eq 120 4 also also RB cord-310467-v2wlu5eq 120 5 obtained obtain VBN cord-310467-v2wlu5eq 120 6 ( ( -LRB- cord-310467-v2wlu5eq 120 7 Groen Groen NNP cord-310467-v2wlu5eq 120 8 et et FW cord-310467-v2wlu5eq 120 9 al al NNP cord-310467-v2wlu5eq 120 10 . . NNP cord-310467-v2wlu5eq 120 11 , , , cord-310467-v2wlu5eq 120 12 1989 1989 CD cord-310467-v2wlu5eq 120 13 ) ) -RRB- cord-310467-v2wlu5eq 120 14 by by IN cord-310467-v2wlu5eq 120 15 comparing compare VBG cord-310467-v2wlu5eq 120 16 the the DT cord-310467-v2wlu5eq 120 17 use use NN cord-310467-v2wlu5eq 120 18 of of IN cord-310467-v2wlu5eq 120 19 ethanol ethanol NN cord-310467-v2wlu5eq 120 20 ( ( -LRB- cord-310467-v2wlu5eq 120 21 -70 -70 NN cord-310467-v2wlu5eq 120 22 ° ° NNP cord-310467-v2wlu5eq 120 23 C C NNP cord-310467-v2wlu5eq 120 24 ) ) -RRB- cord-310467-v2wlu5eq 120 25 or or CC cord-310467-v2wlu5eq 120 26 acetone acetone NN cord-310467-v2wlu5eq 120 27 ( ( -LRB- cord-310467-v2wlu5eq 120 28 + + SYM cord-310467-v2wlu5eq 120 29 20 20 CD cord-310467-v2wlu5eq 120 30 ° ° NN cord-310467-v2wlu5eq 120 31 C c NN cord-310467-v2wlu5eq 120 32 ) ) -RRB- cord-310467-v2wlu5eq 120 33 fixation fixation NN cord-310467-v2wlu5eq 120 34 for for IN cord-310467-v2wlu5eq 120 35 the the DT cord-310467-v2wlu5eq 120 36 detection detection NN cord-310467-v2wlu5eq 120 37 of of IN cord-310467-v2wlu5eq 120 38 Hantaan Hantaan NNP cord-310467-v2wlu5eq 120 39 virus virus NN cord-310467-v2wlu5eq 120 40 . . . cord-310467-v2wlu5eq 121 1 In in IN cord-310467-v2wlu5eq 121 2 his -PRON- PRP$ cord-310467-v2wlu5eq 121 3 study study NN cord-310467-v2wlu5eq 121 4 on on IN cord-310467-v2wlu5eq 121 5 the the DT cord-310467-v2wlu5eq 121 6 development development NN cord-310467-v2wlu5eq 121 7 and and CC cord-310467-v2wlu5eq 121 8 evaluation evaluation NN cord-310467-v2wlu5eq 121 9 of of IN cord-310467-v2wlu5eq 121 10 an an DT cord-310467-v2wlu5eq 121 11 enzyme enzyme NN cord-310467-v2wlu5eq 121 12 labelled label VBN cord-310467-v2wlu5eq 121 13 antibody antibody NN cord-310467-v2wlu5eq 121 14 test test NN cord-310467-v2wlu5eq 121 15 for for IN cord-310467-v2wlu5eq 121 16 the the DT cord-310467-v2wlu5eq 121 17 rapid rapid JJ cord-310467-v2wlu5eq 121 18 detection detection NN cord-310467-v2wlu5eq 121 19 of of IN cord-310467-v2wlu5eq 121 20 hog hog NN cord-310467-v2wlu5eq 121 21 cholera cholera NN cord-310467-v2wlu5eq 121 22 antibodies antibody NNS cord-310467-v2wlu5eq 121 23 , , , cord-310467-v2wlu5eq 121 24 Saunders Saunders NNP cord-310467-v2wlu5eq 121 25 ( ( -LRB- cord-310467-v2wlu5eq 121 26 1977 1977 CD cord-310467-v2wlu5eq 121 27 ) ) -RRB- cord-310467-v2wlu5eq 121 28 showed show VBD cord-310467-v2wlu5eq 121 29 that that IN cord-310467-v2wlu5eq 121 30 this this DT cord-310467-v2wlu5eq 121 31 test test NN cord-310467-v2wlu5eq 121 32 gave give VBD cord-310467-v2wlu5eq 121 33 a a DT cord-310467-v2wlu5eq 121 34 good good JJ cord-310467-v2wlu5eq 121 35 result result NN cord-310467-v2wlu5eq 121 36 only only RB cord-310467-v2wlu5eq 121 37 when when WRB cord-310467-v2wlu5eq 121 38 the the DT cord-310467-v2wlu5eq 121 39 fixative fixative NN cord-310467-v2wlu5eq 121 40 solution solution NN cord-310467-v2wlu5eq 121 41 contained contain VBD cord-310467-v2wlu5eq 121 42 less less JJR cord-310467-v2wlu5eq 121 43 than than IN cord-310467-v2wlu5eq 121 44 30 30 CD cord-310467-v2wlu5eq 121 45 % % NN cord-310467-v2wlu5eq 121 46 acetone acetone NN cord-310467-v2wlu5eq 121 47 . . . cord-310467-v2wlu5eq 122 1 It -PRON- PRP cord-310467-v2wlu5eq 122 2 should should MD cord-310467-v2wlu5eq 122 3 be be VB cord-310467-v2wlu5eq 122 4 noted note VBN cord-310467-v2wlu5eq 122 5 that that IN cord-310467-v2wlu5eq 122 6 100 100 CD cord-310467-v2wlu5eq 122 7 % % NN cord-310467-v2wlu5eq 122 8 acetone acetone NN cord-310467-v2wlu5eq 122 9 is be VBZ cord-310467-v2wlu5eq 122 10 a a DT cord-310467-v2wlu5eq 122 11 routinely routinely RB cord-310467-v2wlu5eq 122 12 used use VBN cord-310467-v2wlu5eq 122 13 fixative fixative NN cord-310467-v2wlu5eq 122 14 which which WDT cord-310467-v2wlu5eq 122 15 gives give VBZ cord-310467-v2wlu5eq 122 16 excellent excellent JJ cord-310467-v2wlu5eq 122 17 results result NNS cord-310467-v2wlu5eq 122 18 in in IN cord-310467-v2wlu5eq 122 19 immunohistochemistry immunohistochemistry NN cord-310467-v2wlu5eq 122 20 . . . cord-310467-v2wlu5eq 123 1 In in IN cord-310467-v2wlu5eq 123 2 our -PRON- PRP$ cord-310467-v2wlu5eq 123 3 experiments experiment NNS cord-310467-v2wlu5eq 123 4 , , , cord-310467-v2wlu5eq 123 5 cell cell NN cord-310467-v2wlu5eq 123 6 fixation fixation NN cord-310467-v2wlu5eq 123 7 with with IN cord-310467-v2wlu5eq 123 8 a a DT cord-310467-v2wlu5eq 123 9 high high JJ cord-310467-v2wlu5eq 123 10 concentration concentration NN cord-310467-v2wlu5eq 123 11 of of IN cord-310467-v2wlu5eq 123 12 acetone acetone NN cord-310467-v2wlu5eq 123 13 ( ( -LRB- cord-310467-v2wlu5eq 123 14 80 80 CD cord-310467-v2wlu5eq 123 15 % % NN cord-310467-v2wlu5eq 123 16 ) ) -RRB- cord-310467-v2wlu5eq 123 17 resulted result VBD cord-310467-v2wlu5eq 123 18 in in IN cord-310467-v2wlu5eq 123 19 adequate adequate JJ cord-310467-v2wlu5eq 123 20 detection detection NN cord-310467-v2wlu5eq 123 21 of of IN cord-310467-v2wlu5eq 123 22 TGEV TGEV NNP cord-310467-v2wlu5eq 123 23 antigens antigen NNS cord-310467-v2wlu5eq 123 24 . . . cord-310467-v2wlu5eq 124 1 However however RB cord-310467-v2wlu5eq 124 2 , , , cord-310467-v2wlu5eq 124 3 we -PRON- PRP cord-310467-v2wlu5eq 124 4 could could MD cord-310467-v2wlu5eq 124 5 not not RB cord-310467-v2wlu5eq 124 6 use use VB cord-310467-v2wlu5eq 124 7 higher high JJR cord-310467-v2wlu5eq 124 8 acetone acetone NN cord-310467-v2wlu5eq 124 9 concentrations concentration NNS cord-310467-v2wlu5eq 124 10 to to TO cord-310467-v2wlu5eq 124 11 fix fix VB cord-310467-v2wlu5eq 124 12 the the DT cord-310467-v2wlu5eq 124 13 cells cell NNS cord-310467-v2wlu5eq 124 14 because because IN cord-310467-v2wlu5eq 124 15 the the DT cord-310467-v2wlu5eq 124 16 microtiter microtiter NN cord-310467-v2wlu5eq 124 17 plates plate NNS cord-310467-v2wlu5eq 124 18 would would MD cord-310467-v2wlu5eq 124 19 be be VB cord-310467-v2wlu5eq 124 20 distorted distort VBN cord-310467-v2wlu5eq 124 21 with with IN cord-310467-v2wlu5eq 124 22 acetone acetone NN cord-310467-v2wlu5eq 124 23 concentration concentration NN cord-310467-v2wlu5eq 124 24 higher high JJR cord-310467-v2wlu5eq 124 25 than than IN cord-310467-v2wlu5eq 124 26 80 80 CD cord-310467-v2wlu5eq 124 27 % % NN cord-310467-v2wlu5eq 124 28 or or CC cord-310467-v2wlu5eq 124 29 would would MD cord-310467-v2wlu5eq 124 30 melt melt VB cord-310467-v2wlu5eq 124 31 with with IN cord-310467-v2wlu5eq 124 32 90 90 CD cord-310467-v2wlu5eq 124 33 - - SYM cord-310467-v2wlu5eq 124 34 100 100 CD cord-310467-v2wlu5eq 124 35 % % NN cord-310467-v2wlu5eq 124 36 acetone acetone NN cord-310467-v2wlu5eq 124 37 ( ( -LRB- cord-310467-v2wlu5eq 124 38 personal personal JJ cord-310467-v2wlu5eq 124 39 observation observation NN cord-310467-v2wlu5eq 124 40 ) ) -RRB- cord-310467-v2wlu5eq 124 41 . . . cord-310467-v2wlu5eq 125 1 Therefore therefore RB cord-310467-v2wlu5eq 125 2 , , , cord-310467-v2wlu5eq 125 3 we -PRON- PRP cord-310467-v2wlu5eq 125 4 do do VBP cord-310467-v2wlu5eq 125 5 not not RB cord-310467-v2wlu5eq 125 6 know know VB cord-310467-v2wlu5eq 125 7 if if IN cord-310467-v2wlu5eq 125 8 acetone acetone NN cord-310467-v2wlu5eq 125 9 concentrations concentration NNS cord-310467-v2wlu5eq 125 10 higher high JJR cord-310467-v2wlu5eq 125 11 than than IN cord-310467-v2wlu5eq 125 12 80 80 CD cord-310467-v2wlu5eq 125 13 % % NN cord-310467-v2wlu5eq 125 14 would would MD cord-310467-v2wlu5eq 125 15 give give VB cord-310467-v2wlu5eq 125 16 the the DT cord-310467-v2wlu5eq 125 17 same same JJ cord-310467-v2wlu5eq 125 18 results result NNS cord-310467-v2wlu5eq 125 19 in in IN cord-310467-v2wlu5eq 125 20 fixed fix VBN cord-310467-v2wlu5eq 125 21 - - HYPH cord-310467-v2wlu5eq 125 22 cell cell NN cord-310467-v2wlu5eq 125 23 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 125 24 . . . cord-310467-v2wlu5eq 126 1 A a DT cord-310467-v2wlu5eq 126 2 comparison comparison NN cord-310467-v2wlu5eq 126 3 of of IN cord-310467-v2wlu5eq 126 4 cell cell NN cord-310467-v2wlu5eq 126 5 fixation fixation NN cord-310467-v2wlu5eq 126 6 with with IN cord-310467-v2wlu5eq 126 7 dehydrating dehydrate VBG cord-310467-v2wlu5eq 126 8 fixatives fixative NNS cord-310467-v2wlu5eq 126 9 and and CC cord-310467-v2wlu5eq 126 10 mixtures mixture NNS cord-310467-v2wlu5eq 126 11 of of IN cord-310467-v2wlu5eq 126 12 these these DT cord-310467-v2wlu5eq 126 13 fixatives fixative NNS cord-310467-v2wlu5eq 126 14 ( ( -LRB- cord-310467-v2wlu5eq 126 15 Fig Fig NNP cord-310467-v2wlu5eq 126 16 . . . cord-310467-v2wlu5eq 126 17 3 3 LS cord-310467-v2wlu5eq 126 18 ) ) -RRB- cord-310467-v2wlu5eq 126 19 suggested suggest VBD cord-310467-v2wlu5eq 126 20 that that IN cord-310467-v2wlu5eq 126 21 the the DT cord-310467-v2wlu5eq 126 22 highest high JJS cord-310467-v2wlu5eq 126 23 quantity quantity NN cord-310467-v2wlu5eq 126 24 of of IN cord-310467-v2wlu5eq 126 25 antigen antigen NN cord-310467-v2wlu5eq 126 26 detected detect VBN cord-310467-v2wlu5eq 126 27 was be VBD cord-310467-v2wlu5eq 126 28 in in IN cord-310467-v2wlu5eq 126 29 TGEV TGEV NNP cord-310467-v2wlu5eq 126 30 - - HYPH cord-310467-v2wlu5eq 126 31 infected infect VBN cord-310467-v2wlu5eq 126 32 cells cell NNS cord-310467-v2wlu5eq 126 33 which which WDT cord-310467-v2wlu5eq 126 34 were be VBD cord-310467-v2wlu5eq 126 35 fixed fix VBN cord-310467-v2wlu5eq 126 36 with with IN cord-310467-v2wlu5eq 126 37 either either CC cord-310467-v2wlu5eq 126 38 80 80 CD cord-310467-v2wlu5eq 126 39 % % NN cord-310467-v2wlu5eq 126 40 acetone acetone NN cord-310467-v2wlu5eq 126 41 or or CC cord-310467-v2wlu5eq 126 42 mixtures mixture NNS cord-310467-v2wlu5eq 126 43 of of IN cord-310467-v2wlu5eq 126 44 act act NN cord-310467-v2wlu5eq 126 45 / / SYM cord-310467-v2wlu5eq 126 46 meth meth NNP cord-310467-v2wlu5eq 126 47 or or CC cord-310467-v2wlu5eq 126 48 act act NN cord-310467-v2wlu5eq 126 49 / / SYM cord-310467-v2wlu5eq 126 50 meth meth NN cord-310467-v2wlu5eq 126 51 / / SYM cord-310467-v2wlu5eq 126 52 eth eth NN cord-310467-v2wlu5eq 126 53 . . . cord-310467-v2wlu5eq 127 1 It -PRON- PRP cord-310467-v2wlu5eq 127 2 is be VBZ cord-310467-v2wlu5eq 127 3 possible possible JJ cord-310467-v2wlu5eq 127 4 that that DT cord-310467-v2wlu5eq 127 5 adequate adequate JJ cord-310467-v2wlu5eq 127 6 detection detection NN cord-310467-v2wlu5eq 127 7 of of IN cord-310467-v2wlu5eq 127 8 TGEV TGEV NNP cord-310467-v2wlu5eq 127 9 antigens antigen NNS cord-310467-v2wlu5eq 127 10 in in IN cord-310467-v2wlu5eq 127 11 act/ act/ CD cord-310467-v2wlu5eq 127 12 meth meth NN cord-310467-v2wlu5eq 127 13 - - , cord-310467-v2wlu5eq 127 14 and and CC cord-310467-v2wlu5eq 127 15 act act NN cord-310467-v2wlu5eq 127 16 / / SYM cord-310467-v2wlu5eq 127 17 meth meth NN cord-310467-v2wlu5eq 127 18 / / SYM cord-310467-v2wlu5eq 127 19 eth eth JJ cord-310467-v2wlu5eq 127 20 - - HYPH cord-310467-v2wlu5eq 127 21 fixed fix VBN cord-310467-v2wlu5eq 127 22 cells cell NNS cord-310467-v2wlu5eq 127 23 is be VBZ cord-310467-v2wlu5eq 127 24 due due JJ cord-310467-v2wlu5eq 127 25 to to IN cord-310467-v2wlu5eq 127 26 the the DT cord-310467-v2wlu5eq 127 27 acetone acetone NN cord-310467-v2wlu5eq 127 28 in in IN cord-310467-v2wlu5eq 127 29 the the DT cord-310467-v2wlu5eq 127 30 fixative fixative NN cord-310467-v2wlu5eq 127 31 preparations preparation NNS cord-310467-v2wlu5eq 127 32 since since IN cord-310467-v2wlu5eq 127 33 viral viral JJ cord-310467-v2wlu5eq 127 34 antigens antigen NNS cord-310467-v2wlu5eq 127 35 are be VBP cord-310467-v2wlu5eq 127 36 not not RB cord-310467-v2wlu5eq 127 37 so so RB cord-310467-v2wlu5eq 127 38 readily readily RB cord-310467-v2wlu5eq 127 39 detected detect VBN cord-310467-v2wlu5eq 127 40 in in IN cord-310467-v2wlu5eq 127 41 infected infected JJ cord-310467-v2wlu5eq 127 42 cells cell NNS cord-310467-v2wlu5eq 127 43 which which WDT cord-310467-v2wlu5eq 127 44 are be VBP cord-310467-v2wlu5eq 127 45 fixed fix VBN cord-310467-v2wlu5eq 127 46 with with IN cord-310467-v2wlu5eq 127 47 methanol methanol NN cord-310467-v2wlu5eq 127 48 or or CC cord-310467-v2wlu5eq 127 49 ethanol ethanol NN cord-310467-v2wlu5eq 127 50 alone alone RB cord-310467-v2wlu5eq 127 51 . . . cord-310467-v2wlu5eq 128 1 Similarly similarly RB cord-310467-v2wlu5eq 128 2 , , , cord-310467-v2wlu5eq 128 3 comparisons comparison NNS cord-310467-v2wlu5eq 128 4 of of IN cord-310467-v2wlu5eq 128 5 cell cell NN cord-310467-v2wlu5eq 128 6 treatment treatment NN cord-310467-v2wlu5eq 128 7 with with IN cord-310467-v2wlu5eq 128 8 permeabilizing permeabilize VBG cord-310467-v2wlu5eq 128 9 agents agent NNS cord-310467-v2wlu5eq 128 10 ( ( -LRB- cord-310467-v2wlu5eq 128 11 Figs fig NNS cord-310467-v2wlu5eq 128 12 . . . cord-310467-v2wlu5eq 128 13 4 4 CD cord-310467-v2wlu5eq 128 14 , , , cord-310467-v2wlu5eq 128 15 5 5 CD cord-310467-v2wlu5eq 128 16 and and CC cord-310467-v2wlu5eq 128 17 6 6 CD cord-310467-v2wlu5eq 128 18 ) ) -RRB- cord-310467-v2wlu5eq 128 19 lead lead NN cord-310467-v2wlu5eq 128 20 to to IN cord-310467-v2wlu5eq 128 21 the the DT cord-310467-v2wlu5eq 128 22 conclusion conclusion NN cord-310467-v2wlu5eq 128 23 that that IN cord-310467-v2wlu5eq 128 24 the the DT cord-310467-v2wlu5eq 128 25 TGEV TGEV NNP cord-310467-v2wlu5eq 128 26 antigens antigen NNS cord-310467-v2wlu5eq 128 27 were be VBD cord-310467-v2wlu5eq 128 28 readily readily RB cord-310467-v2wlu5eq 128 29 detected detect VBN cord-310467-v2wlu5eq 128 30 in in IN cord-310467-v2wlu5eq 128 31 PFA PFA NNP cord-310467-v2wlu5eq 128 32 - - HYPH cord-310467-v2wlu5eq 128 33 fixed fix VBN cord-310467-v2wlu5eq 128 34 cells cell NNS cord-310467-v2wlu5eq 128 35 which which WDT cord-310467-v2wlu5eq 128 36 were be VBD cord-310467-v2wlu5eq 128 37 then then RB cord-310467-v2wlu5eq 128 38 permeated permeate VBN cord-310467-v2wlu5eq 128 39 with with IN cord-310467-v2wlu5eq 128 40 1 1 CD cord-310467-v2wlu5eq 128 41 % % NN cord-310467-v2wlu5eq 128 42 NP-40 NP-40 NNP cord-310467-v2wlu5eq 128 43 and and CC cord-310467-v2wlu5eq 128 44 in in IN cord-310467-v2wlu5eq 128 45 GA GA NNP cord-310467-v2wlu5eq 128 46 - - HYPH cord-310467-v2wlu5eq 128 47 fixed fix VBN cord-310467-v2wlu5eq 128 48 cells cell NNS cord-310467-v2wlu5eq 128 49 which which WDT cord-310467-v2wlu5eq 128 50 were be VBD cord-310467-v2wlu5eq 128 51 then then RB cord-310467-v2wlu5eq 128 52 permeated permeate VBN cord-310467-v2wlu5eq 128 53 with with IN cord-310467-v2wlu5eq 128 54 0.1 0.1 CD cord-310467-v2wlu5eq 128 55 % % NN cord-310467-v2wlu5eq 128 56 saponin saponin NN cord-310467-v2wlu5eq 128 57 . . . cord-310467-v2wlu5eq 129 1 Several several JJ cord-310467-v2wlu5eq 129 2 authors author NNS cord-310467-v2wlu5eq 129 3 have have VBP cord-310467-v2wlu5eq 129 4 compared compare VBN cord-310467-v2wlu5eq 129 5 the the DT cord-310467-v2wlu5eq 129 6 effects effect NNS cord-310467-v2wlu5eq 129 7 of of IN cord-310467-v2wlu5eq 129 8 aldehyde aldehyde NN cord-310467-v2wlu5eq 129 9 fixative fixative NN cord-310467-v2wlu5eq 129 10 and and CC cord-310467-v2wlu5eq 129 11 dehydrating dehydrate VBG cord-310467-v2wlu5eq 129 12 fixative fixative NN cord-310467-v2wlu5eq 129 13 in in IN cord-310467-v2wlu5eq 129 14 the the DT cord-310467-v2wlu5eq 129 15 fixed fix VBN cord-310467-v2wlu5eq 129 16 - - HYPH cord-310467-v2wlu5eq 129 17 cell cell NN cord-310467-v2wlu5eq 129 18 ELISA ELISA NNP cord-310467-v2wlu5eq 129 19 . . . cord-310467-v2wlu5eq 130 1 Cannon Cannon NNP cord-310467-v2wlu5eq 130 2 ( ( -LRB- cord-310467-v2wlu5eq 130 3 1987 1987 CD cord-310467-v2wlu5eq 130 4 ) ) -RRB- cord-310467-v2wlu5eq 130 5 found find VBD cord-310467-v2wlu5eq 130 6 that that IN cord-310467-v2wlu5eq 130 7 both both DT cord-310467-v2wlu5eq 130 8 methanol methanol NN cord-310467-v2wlu5eq 130 9 containing contain VBG cord-310467-v2wlu5eq 130 10 0.5 0.5 CD cord-310467-v2wlu5eq 130 11 % % NN cord-310467-v2wlu5eq 130 12 H20 H20 NNP cord-310467-v2wlu5eq 130 13 2 2 CD cord-310467-v2wlu5eq 130 14 and and CC cord-310467-v2wlu5eq 130 15 4 4 CD cord-310467-v2wlu5eq 130 16 % % NN cord-310467-v2wlu5eq 130 17 FA FA NNP cord-310467-v2wlu5eq 130 18 were be VBD cord-310467-v2wlu5eq 130 19 suitable suitable JJ cord-310467-v2wlu5eq 130 20 fixatives fixative NNS cord-310467-v2wlu5eq 130 21 for for IN cord-310467-v2wlu5eq 130 22 HEp-2 HEp-2 NNP cord-310467-v2wlu5eq 130 23 cells cell NNS cord-310467-v2wlu5eq 130 24 infected infect VBN cord-310467-v2wlu5eq 130 25 with with IN cord-310467-v2wlu5eq 130 26 respiratory respiratory JJ cord-310467-v2wlu5eq 130 27 syncytial syncytial JJ cord-310467-v2wlu5eq 130 28 virus virus NN cord-310467-v2wlu5eq 130 29 ( ( -LRB- cord-310467-v2wlu5eq 130 30 RSV RSV NNP cord-310467-v2wlu5eq 130 31 ) ) -RRB- cord-310467-v2wlu5eq 130 32 , , , cord-310467-v2wlu5eq 130 33 although although IN cord-310467-v2wlu5eq 130 34 methanol methanol NN cord-310467-v2wlu5eq 130 35 / / SYM cord-310467-v2wlu5eq 130 36 H20 H20 NNP cord-310467-v2wlu5eq 130 37 2 2 CD cord-310467-v2wlu5eq 130 38 was be VBD cord-310467-v2wlu5eq 130 39 considered consider VBN cord-310467-v2wlu5eq 130 40 to to TO cord-310467-v2wlu5eq 130 41 be be VB cord-310467-v2wlu5eq 130 42 the the DT cord-310467-v2wlu5eq 130 43 fixative fixative NN cord-310467-v2wlu5eq 130 44 of of IN cord-310467-v2wlu5eq 130 45 choice choice NN cord-310467-v2wlu5eq 130 46 . . . cord-310467-v2wlu5eq 131 1 The the DT cord-310467-v2wlu5eq 131 2 author author NN cord-310467-v2wlu5eq 131 3 also also RB cord-310467-v2wlu5eq 131 4 found find VBD cord-310467-v2wlu5eq 131 5 that that IN cord-310467-v2wlu5eq 131 6 where where WRB cord-310467-v2wlu5eq 131 7 a a DT cord-310467-v2wlu5eq 131 8 MAb MAb NNS cord-310467-v2wlu5eq 131 9 specific specific JJ cord-310467-v2wlu5eq 131 10 for for IN cord-310467-v2wlu5eq 131 11 virus virus NN cord-310467-v2wlu5eq 131 12 NP NP NNP cord-310467-v2wlu5eq 131 13 was be VBD cord-310467-v2wlu5eq 131 14 used use VBN cord-310467-v2wlu5eq 131 15 in in IN cord-310467-v2wlu5eq 131 16 liT liT NNP cord-310467-v2wlu5eq 131 17 , , , cord-310467-v2wlu5eq 131 18 staining staining NN cord-310467-v2wlu5eq 131 19 was be VBD cord-310467-v2wlu5eq 131 20 only only RB cord-310467-v2wlu5eq 131 21 seen see VBN cord-310467-v2wlu5eq 131 22 following follow VBG cord-310467-v2wlu5eq 131 23 FA fa NN cord-310467-v2wlu5eq 131 24 fixation fixation NN cord-310467-v2wlu5eq 131 25 but but CC cord-310467-v2wlu5eq 131 26 this this DT cord-310467-v2wlu5eq 131 27 was be VBD cord-310467-v2wlu5eq 131 28 not not RB cord-310467-v2wlu5eq 131 29 the the DT cord-310467-v2wlu5eq 131 30 case case NN cord-310467-v2wlu5eq 131 31 for for IN cord-310467-v2wlu5eq 131 32 MAb MAb NNS cord-310467-v2wlu5eq 131 33 specific specific JJ cord-310467-v2wlu5eq 131 34 for for IN cord-310467-v2wlu5eq 131 35 RSV RSV NNP cord-310467-v2wlu5eq 131 36 envelope envelope NN cord-310467-v2wlu5eq 131 37 glycoproteins glycoprotein NNS cord-310467-v2wlu5eq 131 38 . . . cord-310467-v2wlu5eq 132 1 The the DT cord-310467-v2wlu5eq 132 2 latter latter JJ cord-310467-v2wlu5eq 132 3 generally generally RB cord-310467-v2wlu5eq 132 4 gave give VBD cord-310467-v2wlu5eq 132 5 good good JJ cord-310467-v2wlu5eq 132 6 results result NNS cord-310467-v2wlu5eq 132 7 with with IN cord-310467-v2wlu5eq 132 8 either either DT cord-310467-v2wlu5eq 132 9 fixative fixative NN cord-310467-v2wlu5eq 132 10 . . . cord-310467-v2wlu5eq 133 1 Similarly similarly RB cord-310467-v2wlu5eq 133 2 , , , cord-310467-v2wlu5eq 133 3 FA FA NNP cord-310467-v2wlu5eq 133 4 fixation fixation NN cord-310467-v2wlu5eq 133 5 seemed seem VBD cord-310467-v2wlu5eq 133 6 to to TO cord-310467-v2wlu5eq 133 7 preserve preserve VB cord-310467-v2wlu5eq 133 8 Borna Borna NNP cord-310467-v2wlu5eq 133 9 virus virus NN cord-310467-v2wlu5eq 133 10 - - HYPH cord-310467-v2wlu5eq 133 11 specific specific JJ cord-310467-v2wlu5eq 133 12 antigens antigen NNS cord-310467-v2wlu5eq 133 13 better well RBR cord-310467-v2wlu5eq 133 14 than than IN cord-310467-v2wlu5eq 133 15 acetone acetone NN cord-310467-v2wlu5eq 133 16 / / SYM cord-310467-v2wlu5eq 133 17 methanol methanol NN cord-310467-v2wlu5eq 133 18 or or CC cord-310467-v2wlu5eq 133 19 acetone acetone NN cord-310467-v2wlu5eq 133 20 fixation fixation NN cord-310467-v2wlu5eq 133 21 and and CC cord-310467-v2wlu5eq 133 22 the the DT cord-310467-v2wlu5eq 133 23 labelling labelling NN cord-310467-v2wlu5eq 133 24 was be VBD cord-310467-v2wlu5eq 133 25 pronounced pronounce VBN cord-310467-v2wlu5eq 133 26 when when WRB cord-310467-v2wlu5eq 133 27 using use VBG cord-310467-v2wlu5eq 133 28 MAb MAb NNS cord-310467-v2wlu5eq 133 29 ( ( -LRB- cord-310467-v2wlu5eq 133 30 Pauli Pauli NNP cord-310467-v2wlu5eq 133 31 et et NNP cord-310467-v2wlu5eq 133 32 al al NNP cord-310467-v2wlu5eq 133 33 . . NNP cord-310467-v2wlu5eq 133 34 , , , cord-310467-v2wlu5eq 133 35 1984 1984 CD cord-310467-v2wlu5eq 133 36 ) ) -RRB- cord-310467-v2wlu5eq 133 37 . . . cord-310467-v2wlu5eq 134 1 Smith Smith NNP cord-310467-v2wlu5eq 134 2 et et NNP cord-310467-v2wlu5eq 134 3 al al NNP cord-310467-v2wlu5eq 134 4 . . . cord-310467-v2wlu5eq 135 1 ( ( -LRB- cord-310467-v2wlu5eq 135 2 1988 1988 CD cord-310467-v2wlu5eq 135 3 ) ) -RRB- cord-310467-v2wlu5eq 135 4 also also RB cord-310467-v2wlu5eq 135 5 noted note VBD cord-310467-v2wlu5eq 135 6 that that IN cord-310467-v2wlu5eq 135 7 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 135 8 staining staining NN cord-310467-v2wlu5eq 135 9 of of IN cord-310467-v2wlu5eq 135 10 BVDV BVDV NNP cord-310467-v2wlu5eq 135 11 - - HYPH cord-310467-v2wlu5eq 135 12 infected infect VBN cord-310467-v2wlu5eq 135 13 cells cell NNS cord-310467-v2wlu5eq 135 14 fixed fix VBN cord-310467-v2wlu5eq 135 15 with with IN cord-310467-v2wlu5eq 135 16 formaldehyde formaldehyde NN cord-310467-v2wlu5eq 135 17 was be VBD cord-310467-v2wlu5eq 135 18 less less RBR cord-310467-v2wlu5eq 135 19 intense intense JJ cord-310467-v2wlu5eq 135 20 in in IN cord-310467-v2wlu5eq 135 21 cells cell NNS cord-310467-v2wlu5eq 135 22 fixed fix VBN cord-310467-v2wlu5eq 135 23 in in IN cord-310467-v2wlu5eq 135 24 acetone acetone NN cord-310467-v2wlu5eq 135 25 . . . cord-310467-v2wlu5eq 136 1 Our -PRON- PRP$ cord-310467-v2wlu5eq 136 2 results result NNS cord-310467-v2wlu5eq 136 3 ( ( -LRB- cord-310467-v2wlu5eq 136 4 Fig Fig NNP cord-310467-v2wlu5eq 136 5 . . NNP cord-310467-v2wlu5eq 136 6 7 7 CD cord-310467-v2wlu5eq 136 7 ) ) -RRB- cord-310467-v2wlu5eq 136 8 showed show VBD cord-310467-v2wlu5eq 136 9 that that IN cord-310467-v2wlu5eq 136 10 only only JJ cord-310467-v2wlu5eq 136 11 viral viral JJ cord-310467-v2wlu5eq 136 12 antigens antigen NNS cord-310467-v2wlu5eq 136 13 which which WDT cord-310467-v2wlu5eq 136 14 are be VBP cord-310467-v2wlu5eq 136 15 present present JJ cord-310467-v2wlu5eq 136 16 on on IN cord-310467-v2wlu5eq 136 17 the the DT cord-310467-v2wlu5eq 136 18 membrane membrane NN cord-310467-v2wlu5eq 136 19 of of IN cord-310467-v2wlu5eq 136 20 infected infected JJ cord-310467-v2wlu5eq 136 21 cells cell NNS cord-310467-v2wlu5eq 136 22 may may MD cord-310467-v2wlu5eq 136 23 be be VB cord-310467-v2wlu5eq 136 24 detected detect VBN cord-310467-v2wlu5eq 136 25 after after IN cord-310467-v2wlu5eq 136 26 aldehyde aldehyde NN cord-310467-v2wlu5eq 136 27 fixation fixation NN cord-310467-v2wlu5eq 136 28 and and CC cord-310467-v2wlu5eq 136 29 that that IN cord-310467-v2wlu5eq 136 30 the the DT cord-310467-v2wlu5eq 136 31 accessibility accessibility NN cord-310467-v2wlu5eq 136 32 of of IN cord-310467-v2wlu5eq 136 33 these these DT cord-310467-v2wlu5eq 136 34 antigenic antigenic JJ cord-310467-v2wlu5eq 136 35 determinants determinant NNS cord-310467-v2wlu5eq 136 36 to to IN cord-310467-v2wlu5eq 136 37 the the DT cord-310467-v2wlu5eq 136 38 appropriate appropriate JJ cord-310467-v2wlu5eq 136 39 antibodies antibody NNS cord-310467-v2wlu5eq 136 40 was be VBD cord-310467-v2wlu5eq 136 41 not not RB cord-310467-v2wlu5eq 136 42 changed change VBN cord-310467-v2wlu5eq 136 43 after after IN cord-310467-v2wlu5eq 136 44 PFA PFA NNP cord-310467-v2wlu5eq 136 45 fixation fixation NN cord-310467-v2wlu5eq 136 46 . . . cord-310467-v2wlu5eq 137 1 However however RB cord-310467-v2wlu5eq 137 2 , , , cord-310467-v2wlu5eq 137 3 both both DT cord-310467-v2wlu5eq 137 4 surface surface NN cord-310467-v2wlu5eq 137 5 and and CC cord-310467-v2wlu5eq 137 6 cytoplasmic cytoplasmic JJ cord-310467-v2wlu5eq 137 7 antigens antigen NNS cord-310467-v2wlu5eq 137 8 were be VBD cord-310467-v2wlu5eq 137 9 detected detect VBN cord-310467-v2wlu5eq 137 10 after after IN cord-310467-v2wlu5eq 137 11 acetone acetone NN cord-310467-v2wlu5eq 137 12 fixation fixation NN cord-310467-v2wlu5eq 137 13 . . . cord-310467-v2wlu5eq 138 1 This this DT cord-310467-v2wlu5eq 138 2 is be VBZ cord-310467-v2wlu5eq 138 3 due due JJ cord-310467-v2wlu5eq 138 4 to to IN cord-310467-v2wlu5eq 138 5 the the DT cord-310467-v2wlu5eq 138 6 fact fact NN cord-310467-v2wlu5eq 138 7 that that IN cord-310467-v2wlu5eq 138 8 after after IN cord-310467-v2wlu5eq 138 9 PFA PFA NNP cord-310467-v2wlu5eq 138 10 fixation fixation NN cord-310467-v2wlu5eq 138 11 me -PRON- PRP cord-310467-v2wlu5eq 138 12 cell cell NN cord-310467-v2wlu5eq 138 13 membrane membrane NN cord-310467-v2wlu5eq 138 14 was be VBD cord-310467-v2wlu5eq 138 15 still still RB cord-310467-v2wlu5eq 138 16 intact intact JJ cord-310467-v2wlu5eq 138 17 but but CC cord-310467-v2wlu5eq 138 18 became become VBD cord-310467-v2wlu5eq 138 19 permeable permeable JJ cord-310467-v2wlu5eq 138 20 after after IN cord-310467-v2wlu5eq 138 21 acetone acetone NN cord-310467-v2wlu5eq 138 22 fixation fixation NN cord-310467-v2wlu5eq 138 23 . . . cord-310467-v2wlu5eq 139 1 Thus thus RB cord-310467-v2wlu5eq 139 2 MAbs MAbs NNP cord-310467-v2wlu5eq 139 3 also also RB cord-310467-v2wlu5eq 139 4 bind bind VBP cord-310467-v2wlu5eq 139 5 to to IN cord-310467-v2wlu5eq 139 6 cytoplasmic cytoplasmic JJ cord-310467-v2wlu5eq 139 7 viral viral JJ cord-310467-v2wlu5eq 139 8 antigens antigen NNS cord-310467-v2wlu5eq 139 9 ( ( -LRB- cord-310467-v2wlu5eq 139 10 T6 T6 NNP cord-310467-v2wlu5eq 139 11 et et NNP cord-310467-v2wlu5eq 139 12 al al NNP cord-310467-v2wlu5eq 139 13 , , , cord-310467-v2wlu5eq 139 14 1991 1991 CD cord-310467-v2wlu5eq 139 15 ) ) -RRB- cord-310467-v2wlu5eq 139 16 . . . cord-310467-v2wlu5eq 140 1 It -PRON- PRP cord-310467-v2wlu5eq 140 2 seems seem VBZ cord-310467-v2wlu5eq 140 3 that that IN cord-310467-v2wlu5eq 140 4 acetone acetone NN cord-310467-v2wlu5eq 140 5 fixation fixation NN cord-310467-v2wlu5eq 140 6 at at IN cord-310467-v2wlu5eq 140 7 -20 -20 NNP cord-310467-v2wlu5eq 140 8 ° ° NNP cord-310467-v2wlu5eq 140 9 ( ( -LRB- cord-310467-v2wlu5eq 140 10 : : : cord-310467-v2wlu5eq 140 11 had have VBD cord-310467-v2wlu5eq 140 12 no no DT cord-310467-v2wlu5eq 140 13 effect effect NN cord-310467-v2wlu5eq 140 14 on on IN cord-310467-v2wlu5eq 140 15 the the DT cord-310467-v2wlu5eq 140 16 accessibility accessibility NN cord-310467-v2wlu5eq 140 17 of of IN cord-310467-v2wlu5eq 140 18 the the DT cord-310467-v2wlu5eq 140 19 N n CD cord-310467-v2wlu5eq 140 20 determinant determinant NN cord-310467-v2wlu5eq 140 21 to to IN cord-310467-v2wlu5eq 140 22 the the DT cord-310467-v2wlu5eq 140 23 MAb MAb NNS cord-310467-v2wlu5eq 140 24 . . . cord-310467-v2wlu5eq 141 1 The the DT cord-310467-v2wlu5eq 141 2 same same JJ cord-310467-v2wlu5eq 141 3 results result NNS cord-310467-v2wlu5eq 141 4 ( ( -LRB- cord-310467-v2wlu5eq 141 5 data datum NNS cord-310467-v2wlu5eq 141 6 not not RB cord-310467-v2wlu5eq 141 7 shown show VBN cord-310467-v2wlu5eq 141 8 ) ) -RRB- cord-310467-v2wlu5eq 141 9 were be VBD cord-310467-v2wlu5eq 141 10 also also RB cord-310467-v2wlu5eq 141 11 obtained obtain VBN cord-310467-v2wlu5eq 141 12 in in IN cord-310467-v2wlu5eq 141 13 TGEV TGEV NNP cord-310467-v2wlu5eq 141 14 - - HYPH cord-310467-v2wlu5eq 141 15 infected infect VBN cord-310467-v2wlu5eq 141 16 cells cell NNS cord-310467-v2wlu5eq 141 17 which which WDT cord-310467-v2wlu5eq 141 18 were be VBD cord-310467-v2wlu5eq 141 19 fixed fix VBN cord-310467-v2wlu5eq 141 20 with with IN cord-310467-v2wlu5eq 141 21 either either DT cord-310467-v2wlu5eq 141 22 act act NN cord-310467-v2wlu5eq 141 23 / / SYM cord-310467-v2wlu5eq 141 24 meth meth NN cord-310467-v2wlu5eq 141 25 or or CC cord-310467-v2wlu5eq 141 26 act act NN cord-310467-v2wlu5eq 141 27 / / SYM cord-310467-v2wlu5eq 141 28 meth meth NNP cord-310467-v2wlu5eq 141 29 / / SYM cord-310467-v2wlu5eq 141 30 eth eth NN cord-310467-v2wlu5eq 141 31 using use VBG cord-310467-v2wlu5eq 141 32 two two CD cord-310467-v2wlu5eq 141 33 different different JJ cord-310467-v2wlu5eq 141 34 fixation fixation NN cord-310467-v2wlu5eq 141 35 procedures procedure NNS cord-310467-v2wlu5eq 141 36 . . . cord-310467-v2wlu5eq 142 1 It -PRON- PRP cord-310467-v2wlu5eq 142 2 would would MD cord-310467-v2wlu5eq 142 3 seem seem VB cord-310467-v2wlu5eq 142 4 that that IN cord-310467-v2wlu5eq 142 5 the the DT cord-310467-v2wlu5eq 142 6 stronger strong JJR cord-310467-v2wlu5eq 142 7 the the DT cord-310467-v2wlu5eq 142 8 permeabilizing permeabilizing NN cord-310467-v2wlu5eq 142 9 capacity capacity NN cord-310467-v2wlu5eq 142 10 of of IN cord-310467-v2wlu5eq 142 11 the the DT cord-310467-v2wlu5eq 142 12 fixative fixative NN cord-310467-v2wlu5eq 142 13 , , , cord-310467-v2wlu5eq 142 14 the the DT cord-310467-v2wlu5eq 142 15 more more JJR cord-310467-v2wlu5eq 142 16 N n CD cord-310467-v2wlu5eq 142 17 antigen antigen NN cord-310467-v2wlu5eq 142 18 but but CC cord-310467-v2wlu5eq 142 19 less less JJR cord-310467-v2wlu5eq 142 20 M M NNP cord-310467-v2wlu5eq 142 21 and and CC cord-310467-v2wlu5eq 142 22 S S NNP cord-310467-v2wlu5eq 142 23 antigens antigen NNS cord-310467-v2wlu5eq 142 24 were be VBD cord-310467-v2wlu5eq 142 25 detected detect VBN cord-310467-v2wlu5eq 142 26 . . . cord-310467-v2wlu5eq 143 1 Consequently consequently RB cord-310467-v2wlu5eq 143 2 , , , cord-310467-v2wlu5eq 143 3 the the DT cord-310467-v2wlu5eq 143 4 interpretation interpretation NN cord-310467-v2wlu5eq 143 5 of of IN cord-310467-v2wlu5eq 143 6 results result NNS cord-310467-v2wlu5eq 143 7 comparing compare VBG cord-310467-v2wlu5eq 143 8 aldehyde aldehyde NN cord-310467-v2wlu5eq 143 9 and and CC cord-310467-v2wlu5eq 143 10 dehydrating dehydrate VBG cord-310467-v2wlu5eq 143 11 fixatives fixative NNS cord-310467-v2wlu5eq 143 12 should should MD cord-310467-v2wlu5eq 143 13 take take VB cord-310467-v2wlu5eq 143 14 this this DT cord-310467-v2wlu5eq 143 15 observation observation NN cord-310467-v2wlu5eq 143 16 into into IN cord-310467-v2wlu5eq 143 17 consideration consideration NN cord-310467-v2wlu5eq 143 18 . . . cord-310467-v2wlu5eq 144 1 In in IN cord-310467-v2wlu5eq 144 2 conclusion conclusion NN cord-310467-v2wlu5eq 144 3 0.1 0.1 CD cord-310467-v2wlu5eq 144 4 % % NN cord-310467-v2wlu5eq 144 5 PFA PFA NNP cord-310467-v2wlu5eq 144 6 appears appear VBZ cord-310467-v2wlu5eq 144 7 to to TO cord-310467-v2wlu5eq 144 8 be be VB cord-310467-v2wlu5eq 144 9 a a DT cord-310467-v2wlu5eq 144 10 suitable suitable JJ cord-310467-v2wlu5eq 144 11 fixative fixative NN cord-310467-v2wlu5eq 144 12 for for IN cord-310467-v2wlu5eq 144 13 the the DT cord-310467-v2wlu5eq 144 14 detection detection NN cord-310467-v2wlu5eq 144 15 of of IN cord-310467-v2wlu5eq 144 16 TGEV TGEV NNP cord-310467-v2wlu5eq 144 17 antigens antigen NNS cord-310467-v2wlu5eq 144 18 on on IN cord-310467-v2wlu5eq 144 19 the the DT cord-310467-v2wlu5eq 144 20 membrane membrane NN cord-310467-v2wlu5eq 144 21 of of IN cord-310467-v2wlu5eq 144 22 infected infected JJ cord-310467-v2wlu5eq 144 23 cells cell NNS cord-310467-v2wlu5eq 144 24 . . . cord-310467-v2wlu5eq 145 1 For for IN cord-310467-v2wlu5eq 145 2 cellular cellular JJ cord-310467-v2wlu5eq 145 3 viral viral JJ cord-310467-v2wlu5eq 145 4 antigens antigen NNS cord-310467-v2wlu5eq 145 5 ; ; : cord-310467-v2wlu5eq 145 6 80 80 CD cord-310467-v2wlu5eq 145 7 % % NN cord-310467-v2wlu5eq 145 8 acetone acetone NN cord-310467-v2wlu5eq 145 9 , , , cord-310467-v2wlu5eq 145 10 a a DT cord-310467-v2wlu5eq 145 11 mixture mixture NN cord-310467-v2wlu5eq 145 12 of of IN cord-310467-v2wlu5eq 145 13 act act NN cord-310467-v2wlu5eq 145 14 / / SYM cord-310467-v2wlu5eq 145 15 meth meth NNP cord-310467-v2wlu5eq 145 16 or or CC cord-310467-v2wlu5eq 145 17 act act NN cord-310467-v2wlu5eq 145 18 / / SYM cord-310467-v2wlu5eq 145 19 meth meth NNP cord-310467-v2wlu5eq 145 20 / / SYM cord-310467-v2wlu5eq 145 21 eth eth NNP cord-310467-v2wlu5eq 145 22 are be VBP cord-310467-v2wlu5eq 145 23 the the DT cord-310467-v2wlu5eq 145 24 fixatives fixative NNS cord-310467-v2wlu5eq 145 25 of of IN cord-310467-v2wlu5eq 145 26 choice choice NN cord-310467-v2wlu5eq 145 27 . . . cord-310467-v2wlu5eq 146 1 Identification identification NN cord-310467-v2wlu5eq 146 2 of of IN cord-310467-v2wlu5eq 146 3 epitopes epitope NNS cord-310467-v2wlu5eq 146 4 on on IN cord-310467-v2wlu5eq 146 5 respiratory respiratory JJ cord-310467-v2wlu5eq 146 6 syncytial syncytial JJ cord-310467-v2wlu5eq 146 7 virus virus NN cord-310467-v2wlu5eq 146 8 proteins protein NNS cord-310467-v2wlu5eq 146 9 by by IN cord-310467-v2wlu5eq 146 10 competitive competitive JJ cord-310467-v2wlu5eq 146 11 linking linking NN cord-310467-v2wlu5eq 146 12 immunoassay immunoassay JJ cord-310467-v2wlu5eq 146 13 Transmissible transmissible JJ cord-310467-v2wlu5eq 146 14 gastrnenteritis gastrnenteritis NN cord-310467-v2wlu5eq 146 15 ( ( -LRB- cord-310467-v2wlu5eq 146 16 TGE TGE NNP cord-310467-v2wlu5eq 146 17 ) ) -RRB- cord-310467-v2wlu5eq 146 18 of of IN cord-310467-v2wlu5eq 146 19 swine swine NN cord-310467-v2wlu5eq 146 20 : : : cord-310467-v2wlu5eq 146 21 survivor survivor NN cord-310467-v2wlu5eq 146 22 selection selection NN cord-310467-v2wlu5eq 146 23 of of IN cord-310467-v2wlu5eq 146 24 TGE TGE NNP cord-310467-v2wlu5eq 146 25 virus virus NN cord-310467-v2wlu5eq 146 26 mutant mutant JJ cord-310467-v2wlu5eq 146 27 in in IN cord-310467-v2wlu5eq 146 28 stomach stomach NN cord-310467-v2wlu5eq 146 29 juice juice NN cord-310467-v2wlu5eq 146 30 of of IN cord-310467-v2wlu5eq 146 31 adult adult NN cord-310467-v2wlu5eq 146 32 pigs pig NNS cord-310467-v2wlu5eq 146 33 Antigenic antigenic JJ cord-310467-v2wlu5eq 146 34 differences difference NNS cord-310467-v2wlu5eq 146 35 between between IN cord-310467-v2wlu5eq 146 36 virulent virulent JJ cord-310467-v2wlu5eq 146 37 and and CC cord-310467-v2wlu5eq 146 38 avirulent avirulent JJ cord-310467-v2wlu5eq 146 39 strains strain NNS cord-310467-v2wlu5eq 146 40 of of IN cord-310467-v2wlu5eq 146 41 Semliki Semliki NNP cord-310467-v2wlu5eq 146 42 forest forest NN cord-310467-v2wlu5eq 146 43 viruses virus NNS cord-310467-v2wlu5eq 146 44 detected detect VBN cord-310467-v2wlu5eq 146 45 with with IN cord-310467-v2wlu5eq 146 46 mAb mAb NNP cord-310467-v2wlu5eq 146 47 Antibody Antibody NNP cord-310467-v2wlu5eq 146 48 response response NN cord-310467-v2wlu5eq 146 49 in in IN cord-310467-v2wlu5eq 146 50 serum serum NN cord-310467-v2wlu5eq 146 51 , , , cord-310467-v2wlu5eq 146 52 colostrum colostrum NN cord-310467-v2wlu5eq 146 53 and and CC cord-310467-v2wlu5eq 146 54 milk milk NN cord-310467-v2wlu5eq 146 55 of of IN cord-310467-v2wlu5eq 146 56 swine swine NN cord-310467-v2wlu5eq 146 57 after after IN cord-310467-v2wlu5eq 146 58 infection infection NN cord-310467-v2wlu5eq 146 59 or or CC cord-310467-v2wlu5eq 146 60 vaccination vaccination NN cord-310467-v2wlu5eq 146 61 with with IN cord-310467-v2wlu5eq 146 62 transmissible transmissible JJ cord-310467-v2wlu5eq 146 63 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 146 64 virus virus NN cord-310467-v2wlu5eq 146 65 Microplate Microplate NNP cord-310467-v2wlu5eq 146 66 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 146 67 detection detection NN cord-310467-v2wlu5eq 146 68 of of IN cord-310467-v2wlu5eq 146 69 infectious infectious JJ cord-310467-v2wlu5eq 146 70 respirato~ respirato~ NNP cord-310467-v2wlu5eq 146 71 ~ncytial ~ncytial CD cord-310467-v2wlu5eq 146 72 virus virus NN cord-310467-v2wlu5eq 146 73 in in IN cord-310467-v2wlu5eq 146 74 the the DT cord-310467-v2wlu5eq 146 75 lung lung NN cord-310467-v2wlu5eq 146 76 of of IN cord-310467-v2wlu5eq 146 77 infected infected JJ cord-310467-v2wlu5eq 146 78 mice mouse NNS cord-310467-v2wlu5eq 146 79 Comparison comparison NN cord-310467-v2wlu5eq 146 80 of of IN cord-310467-v2wlu5eq 146 81 immunofluorescence immunofluorescence NN cord-310467-v2wlu5eq 146 82 and and CC cord-310467-v2wlu5eq 146 83 enzymelinked enzymelinked JJ cord-310467-v2wlu5eq 146 84 immunosorbent immunosorbent JJ cord-310467-v2wlu5eq 146 85 assays assay NNS cord-310467-v2wlu5eq 146 86 for for IN cord-310467-v2wlu5eq 146 87 the the DT cord-310467-v2wlu5eq 146 88 serology serology NN cord-310467-v2wlu5eq 146 89 of of IN cord-310467-v2wlu5eq 146 90 Hantaan Hantaan NNP cord-310467-v2wlu5eq 146 91 virus virus NN cord-310467-v2wlu5eq 146 92 infections infection NNS cord-310467-v2wlu5eq 146 93 Virus virus NN cord-310467-v2wlu5eq 146 94 enzyme enzyme NN cord-310467-v2wlu5eq 146 95 - - HYPH cord-310467-v2wlu5eq 146 96 linked link VBN cord-310467-v2wlu5eq 146 97 cell cell NN cord-310467-v2wlu5eq 146 98 immunoassay immunoassay NN cord-310467-v2wlu5eq 146 99 ( ( -LRB- cord-310467-v2wlu5eq 146 100 VELCIA VELCIA NNP cord-310467-v2wlu5eq 146 101 ) ) -RRB- cord-310467-v2wlu5eq 146 102 : : : cord-310467-v2wlu5eq 146 103 detection detection NN cord-310467-v2wlu5eq 146 104 and and CC cord-310467-v2wlu5eq 146 105 titration titration NN cord-310467-v2wlu5eq 146 106 of of IN cord-310467-v2wlu5eq 146 107 rotavirus rotavirus JJ cord-310467-v2wlu5eq 146 108 antigen antigen NN cord-310467-v2wlu5eq 146 109 and and CC cord-310467-v2wlu5eq 146 110 demonstration demonstration NN cord-310467-v2wlu5eq 146 111 of of IN cord-310467-v2wlu5eq 146 112 rotavirus rotavirus NN cord-310467-v2wlu5eq 146 113 neutralizing neutralize VBG cord-310467-v2wlu5eq 146 114 and and CC cord-310467-v2wlu5eq 146 115 total total JJ cord-310467-v2wlu5eq 146 116 antibodies antibody NNS cord-310467-v2wlu5eq 146 117 Detection detection NN cord-310467-v2wlu5eq 146 118 of of IN cord-310467-v2wlu5eq 146 119 antibody antibody NN cord-310467-v2wlu5eq 146 120 against against IN cord-310467-v2wlu5eq 146 121 transmissible transmissible JJ cord-310467-v2wlu5eq 146 122 gastroenteritis gastroenteritis JJ cord-310467-v2wlu5eq 146 123 virus virus NN cord-310467-v2wlu5eq 146 124 of of IN cord-310467-v2wlu5eq 146 125 pigs pig NNS cord-310467-v2wlu5eq 146 126 by by IN cord-310467-v2wlu5eq 146 127 indirect indirect JJ cord-310467-v2wlu5eq 146 128 immunoperoxidase immunoperoxidase NNP cord-310467-v2wlu5eq 146 129 antibody antibody NN cord-310467-v2wlu5eq 146 130 test test NN cord-310467-v2wlu5eq 146 131 Characterization characterization NN cord-310467-v2wlu5eq 146 132 of of IN cord-310467-v2wlu5eq 146 133 immunogiobuSn immunogiobuSn NNP cord-310467-v2wlu5eq 146 134 A a DT cord-310467-v2wlu5eq 146 135 antibody antibody NN cord-310467-v2wlu5eq 146 136 in in IN cord-310467-v2wlu5eq 146 137 serum serum NN cord-310467-v2wlu5eq 146 138 of of IN cord-310467-v2wlu5eq 146 139 swine swine NN cord-310467-v2wlu5eq 146 140 inoculated inoculate VBN cord-310467-v2wlu5eq 146 141 with with IN cord-310467-v2wlu5eq 146 142 transmissible transmissible JJ cord-310467-v2wlu5eq 146 143 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 146 144 virus virus NN cord-310467-v2wlu5eq 146 145 1~90 1~90 CD cord-310467-v2wlu5eq 146 146 ) ) -RRB- cord-310467-v2wlu5eq 147 1 Molecular molecular JJ cord-310467-v2wlu5eq 147 2 biology biology NN cord-310467-v2wlu5eq 147 3 of of IN cord-310467-v2wlu5eq 147 4 transmissible transmissible JJ cord-310467-v2wlu5eq 147 5 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 147 6 viru!,~ viru!,~ NN cord-310467-v2wlu5eq 148 1 Antigenic antigenic JJ cord-310467-v2wlu5eq 148 2 ~ ~ NFP cord-310467-v2wlu5eq 148 3 structure structure NN cord-310467-v2wlu5eq 148 4 of of IN cord-310467-v2wlu5eq 148 5 transmissible transmissible JJ cord-310467-v2wlu5eq 148 6 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 148 7 virus virus NN cord-310467-v2wlu5eq 148 8 : : : cord-310467-v2wlu5eq 148 9 1 1 CD cord-310467-v2wlu5eq 148 10 . . . cord-310467-v2wlu5eq 149 1 Properties property NNS cord-310467-v2wlu5eq 149 2 of of IN cord-310467-v2wlu5eq 149 3 mAb mAb NNS cord-310467-v2wlu5eq 149 4 directed direct VBN cord-310467-v2wlu5eq 149 5 against against IN cord-310467-v2wlu5eq 149 6 virion virion NN cord-310467-v2wlu5eq 149 7 proteins protein NNS cord-310467-v2wlu5eq 149 8 Studies study NNS cord-310467-v2wlu5eq 149 9 on on IN cord-310467-v2wlu5eq 149 10 transmissible transmissible JJ cord-310467-v2wlu5eq 149 11 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 149 12 of of IN cord-310467-v2wlu5eq 149 13 swine swine NN cord-310467-v2wlu5eq 149 14 . . . cord-310467-v2wlu5eq 150 1 II II NNP cord-310467-v2wlu5eq 150 2 . . . cord-310467-v2wlu5eq 151 1 Selected select VBN cord-310467-v2wlu5eq 151 2 characteristics characteristic NNS cord-310467-v2wlu5eq 151 3 of of IN cord-310467-v2wlu5eq 151 4 cytopathogenie cytopathogenie NNP cord-310467-v2wlu5eq 151 5 virus virus NN cord-310467-v2wlu5eq 151 6 common common JJ cord-310467-v2wlu5eq 151 7 to to IN cord-310467-v2wlu5eq 151 8 five five CD cord-310467-v2wlu5eq 151 9 isolates isolate NNS cord-310467-v2wlu5eq 151 10 from from IN cord-310467-v2wlu5eq 151 11 transmissible transmissible JJ cord-310467-v2wlu5eq 151 12 gastrocnteritis gastrocnteritis NNP cord-310467-v2wlu5eq 151 13 Plaque Plaque NNP cord-310467-v2wlu5eq 151 14 / / SYM cord-310467-v2wlu5eq 151 15 focus focus NNP cord-310467-v2wlu5eq 151 16 immunoassays immunoassays NNP cord-310467-v2wlu5eq 151 17 : : : cord-310467-v2wlu5eq 151 18 a a DT cord-310467-v2wlu5eq 151 19 simple simple JJ cord-310467-v2wlu5eq 151 20 method method NN cord-310467-v2wlu5eq 151 21 for for IN cord-310467-v2wlu5eq 151 22 detecting detect VBG cord-310467-v2wlu5eq 151 23 antiviral antiviral JJ cord-310467-v2wlu5eq 151 24 monoclonal monoclonal JJ cord-310467-v2wlu5eq 151 25 and and CC cord-310467-v2wlu5eq 151 26 other other JJ cord-310467-v2wlu5eq 151 27 antibodies antibody NNS cord-310467-v2wlu5eq 151 28 and and CC cord-310467-v2wlu5eq 151 29 viral viral JJ cord-310467-v2wlu5eq 151 30 antigens antigen NNS cord-310467-v2wlu5eq 151 31 in in IN cord-310467-v2wlu5eq 151 32 cells cell NNS cord-310467-v2wlu5eq 152 1 A a DT cord-310467-v2wlu5eq 152 2 mierov mierov NN cord-310467-v2wlu5eq 153 1 ~ll ~ll NNP cord-310467-v2wlu5eq 153 2 immunoperoxidase immunoperoxidase NNP cord-310467-v2wlu5eq 153 3 test test NN cord-310467-v2wlu5eq 153 4 for for IN cord-310467-v2wlu5eq 153 5 screening screen VBG cord-310467-v2wlu5eq 153 6 hybridomas hybridomas NN cord-310467-v2wlu5eq 153 7 and and CC cord-310467-v2wlu5eq 153 8 for for IN cord-310467-v2wlu5eq 153 9 diagnosing diagnose VBG cord-310467-v2wlu5eq 153 10 Newcastle Newcastle NNP cord-310467-v2wlu5eq 153 11 disease disease NN cord-310467-v2wlu5eq 153 12 virus virus NN cord-310467-v2wlu5eq 153 13 and and CC cord-310467-v2wlu5eq 153 14 Sendal Sendal NNP cord-310467-v2wlu5eq 153 15 virus virus NN cord-310467-v2wlu5eq 153 16 C c NN cord-310467-v2wlu5eq 153 17 ( ( -LRB- cord-310467-v2wlu5eq 153 18 1977 1977 CD cord-310467-v2wlu5eq 153 19 ) ) -RRB- cord-310467-v2wlu5eq 153 20 Development development NN cord-310467-v2wlu5eq 153 21 and and CC cord-310467-v2wlu5eq 153 22 evaluation evaluation NN cord-310467-v2wlu5eq 153 23 of of IN cord-310467-v2wlu5eq 153 24 an an DT cord-310467-v2wlu5eq 153 25 enzyme enzyme NN cord-310467-v2wlu5eq 153 26 labelled label VBN cord-310467-v2wlu5eq 153 27 antibody antibody NN cord-310467-v2wlu5eq 153 28 test test NN cord-310467-v2wlu5eq 153 29 for for IN cord-310467-v2wlu5eq 153 30 the the DT cord-310467-v2wlu5eq 153 31 rapid rapid JJ cord-310467-v2wlu5eq 153 32 detection detection NN cord-310467-v2wlu5eq 153 33 of of IN cord-310467-v2wlu5eq 153 34 hog hog NN cord-310467-v2wlu5eq 153 35 cholera cholera NN cord-310467-v2wlu5eq 153 36 antibodies antibody NNS cord-310467-v2wlu5eq 153 37 Epitope Epitope NNP cord-310467-v2wlu5eq 153 38 mapping mapping NN cord-310467-v2wlu5eq 153 39 and and CC cord-310467-v2wlu5eq 153 40 detection detection NN cord-310467-v2wlu5eq 153 41 of of IN cord-310467-v2wlu5eq 153 42 transmissible transmissible JJ cord-310467-v2wlu5eq 153 43 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 153 44 viral viral JJ cord-310467-v2wlu5eq 153 45 proteins protein NNS cord-310467-v2wlu5eq 153 46 in in IN cord-310467-v2wlu5eq 153 47 cell cell NN cord-310467-v2wlu5eq 153 48 culture culture NN cord-310467-v2wlu5eq 153 49 using use VBG cord-310467-v2wlu5eq 153 50 biotinylated biotinylate VBN cord-310467-v2wlu5eq 153 51 mAb mAb NNS cord-310467-v2wlu5eq 153 52 in in IN cord-310467-v2wlu5eq 153 53 a a DT cord-310467-v2wlu5eq 153 54 fixed fix VBN cord-310467-v2wlu5eq 153 55 - - HYPH cord-310467-v2wlu5eq 153 56 cen cen NN cord-310467-v2wlu5eq 153 57 ELISA ELISA NNP cord-310467-v2wlu5eq 153 58 Detection Detection NNP cord-310467-v2wlu5eq 153 59 of of IN cord-310467-v2wlu5eq 153 60 cytopathic cytopathic JJ cord-310467-v2wlu5eq 153 61 and and CC cord-310467-v2wlu5eq 153 62 noncythopathic noncythopathic JJ cord-310467-v2wlu5eq 153 63 bovine bovine JJ cord-310467-v2wlu5eq 153 64 viral viral JJ cord-310467-v2wlu5eq 153 65 diarrhea diarrhea NN cord-310467-v2wlu5eq 153 66 virus virus NN cord-310467-v2wlu5eq 153 67 in in IN cord-310467-v2wlu5eq 153 68 cell cell NN cord-310467-v2wlu5eq 153 69 culture culture NN cord-310467-v2wlu5eq 153 70 with with IN cord-310467-v2wlu5eq 153 71 an an DT cord-310467-v2wlu5eq 153 72 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 153 73 test test NN cord-310467-v2wlu5eq 153 74 Techniques technique NNS cord-310467-v2wlu5eq 153 75 immunoenzymatiques immunoenzymatique NNS cord-310467-v2wlu5eq 153 76 . . . cord-310467-v2wlu5eq 154 1 Soci Soci NNP cord-310467-v2wlu5eq 154 2 ~ ~ NFP cord-310467-v2wlu5eq 154 3 t~ t~ ADD cord-310467-v2wlu5eq 155 1 Fran Fran NNP cord-310467-v2wlu5eq 155 2 ~ ~ NNP cord-310467-v2wlu5eq 155 3 aise aise NNP cord-310467-v2wlu5eq 155 4 d'lmmunologie d'lmmunologie NNP cord-310467-v2wlu5eq 155 5 . . . cord-310467-v2wlu5eq 156 1 Les Les NNP cord-310467-v2wlu5eq 156 2 Editions Editions NNP cord-310467-v2wlu5eq 156 3 INSERM INSERM NNP cord-310467-v2wlu5eq 156 4 Fixed Fixed NNP cord-310467-v2wlu5eq 156 5 - - HYPH cord-310467-v2wlu5eq 156 6 ceU ceu JJ cord-310467-v2wlu5eq 156 7 immunoperoxidase immunoperoxidase NN cord-310467-v2wlu5eq 156 8 technique technique NN cord-310467-v2wlu5eq 156 9 for for IN cord-310467-v2wlu5eq 156 10 the the DT cord-310467-v2wlu5eq 156 11 study study NN cord-310467-v2wlu5eq 156 12 of of IN cord-310467-v2wlu5eq 156 13 surface surface NN cord-310467-v2wlu5eq 156 14 antigens antigen NNS cord-310467-v2wlu5eq 156 15 induced induce VBN cord-310467-v2wlu5eq 156 16 by by IN cord-310467-v2wlu5eq 156 17 the the DT cord-310467-v2wlu5eq 156 18 coronavirus coronavirus NN cord-310467-v2wlu5eq 156 19 of of IN cord-310467-v2wlu5eq 156 20 transmissible transmissible JJ cord-310467-v2wlu5eq 156 21 gastruenteritis gastruenteritis NN cord-310467-v2wlu5eq 156 22 ( ( -LRB- cord-310467-v2wlu5eq 156 23 TGEV TGEV NNP cord-310467-v2wlu5eq 156 24 ) ) -RRB- cord-310467-v2wlu5eq 157 1 Enzyme Enzyme NNS cord-310467-v2wlu5eq 157 2 immunoassay immunoassay NN cord-310467-v2wlu5eq 157 3 of of IN cord-310467-v2wlu5eq 157 4 mumps mumps NN cord-310467-v2wlu5eq 157 5 virus virus NN cord-310467-v2wlu5eq 157 6 in in IN cord-310467-v2wlu5eq 157 7 cell cell NN cord-310467-v2wlu5eq 157 8 culture culture NN cord-310467-v2wlu5eq 157 9 with with IN cord-310467-v2wlu5eq 157 10 peroxidaselabelled peroxidaselabelled JJ cord-310467-v2wlu5eq 157 11 virus virus NN cord-310467-v2wlu5eq 157 12 specific specific JJ cord-310467-v2wlu5eq 157 13 monoclonal monoclonal JJ cord-310467-v2wlu5eq 157 14 antibodies antibody NNS cord-310467-v2wlu5eq 157 15 and and CC cord-310467-v2wlu5eq 157 16 its -PRON- PRP$ cord-310467-v2wlu5eq 157 17 application application NN cord-310467-v2wlu5eq 157 18 for for IN cord-310467-v2wlu5eq 157 19 determination determination NN cord-310467-v2wlu5eq 157 20 of of IN cord-310467-v2wlu5eq 157 21 antibodies antibody NNS cord-310467-v2wlu5eq 157 22 Transmissible transmissible JJ cord-310467-v2wlu5eq 157 23 gastroenteritis gastroenteritis NN cord-310467-v2wlu5eq 158 1 We -PRON- PRP cord-310467-v2wlu5eq 158 2 are be VBP cord-310467-v2wlu5eq 158 3 grateful grateful JJ cord-310467-v2wlu5eq 158 4 to to IN cord-310467-v2wlu5eq 158 5 Dr. Dr. NNP cord-310467-v2wlu5eq 158 6 J.M. J.M. NNP cord-310467-v2wlu5eq 158 7 Aynaud Aynaud NNP cord-310467-v2wlu5eq 158 8 for for IN cord-310467-v2wlu5eq 158 9 useful useful JJ cord-310467-v2wlu5eq 158 10 advice advice NN cord-310467-v2wlu5eq 158 11 , , , cord-310467-v2wlu5eq 158 12 to to IN cord-310467-v2wlu5eq 158 13 E. E. NNP cord-310467-v2wlu5eq 158 14 Bottreau Bottreau NNP cord-310467-v2wlu5eq 158 15 and and CC cord-310467-v2wlu5eq 158 16 I. I. NNP cord-310467-v2wlu5eq 158 17 Lantier Lantier NNP cord-310467-v2wlu5eq 158 18 for for IN cord-310467-v2wlu5eq 158 19 help help NN cord-310467-v2wlu5eq 158 20 with with IN cord-310467-v2wlu5eq 158 21 the the DT cord-310467-v2wlu5eq 158 22 cell cell NN cord-310467-v2wlu5eq 158 23 cultures culture NNS cord-310467-v2wlu5eq 158 24 and and CC cord-310467-v2wlu5eq 158 25 ELISA ELISA NNP cord-310467-v2wlu5eq 158 26 . . . cord-310467-v2wlu5eq 159 1 We -PRON- PRP cord-310467-v2wlu5eq 159 2 also also RB cord-310467-v2wlu5eq 159 3 wish wish VBP cord-310467-v2wlu5eq 159 4 to to IN cord-310467-v2wlu5eq 159 5 203 203 CD cord-310467-v2wlu5eq 159 6 acknowledge acknowledge VB cord-310467-v2wlu5eq 159 7 the the DT cord-310467-v2wlu5eq 159 8 Fondation Fondation NNP cord-310467-v2wlu5eq 159 9 MarceI MarceI NNP cord-310467-v2wlu5eq 160 1 -M6rieux -M6rieux NNP cord-310467-v2wlu5eq 160 2 for for IN cord-310467-v2wlu5eq 160 3 providing provide VBG cord-310467-v2wlu5eq 160 4 a a DT cord-310467-v2wlu5eq 160 5 scholarship scholarship NN cord-310467-v2wlu5eq 160 6 to to IN cord-310467-v2wlu5eq 160 7 the the DT cord-310467-v2wlu5eq 160 8 leading lead VBG cord-310467-v2wlu5eq 160 9 author author NN cord-310467-v2wlu5eq 160 10 . . .