id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-287590-jjft3den	Rodák, L.	Use of Monoclonal Antibodies in Blocking ELISA Detection of Transmissible Gastroenteritis Virus in Faeces of Piglets	2005-05-05		.txt	text/plain	3710	215	51	title: Use of Monoclonal Antibodies in Blocking ELISA Detection of Transmissible Gastroenteritis Virus in Faeces of Piglets Monoclonal antibodies (mAb) to the transmissible gastroenteritis virus (TGEV) nucleoprotein (N) and membrane protein (M) were prepared and used for the comparative assessment of three blocking ELISA variants to detect TGEV. Three variants of ELISA method with specificity checked by blocking test were compared by box titration using faeces of experimentally infected piglets 24 hpi. By the use of mAb D7/G7 alone or mixture of mAb in CB-ELISA examination of various TGEV strains (Table 1) or positive field faecal samples (results not shown) the same results were obtained. Therefore, the detection of TGEV in faecal samples by blocking ELISA method was the objective of our study. Competition ELISA, using monoclonal antibodies to the transmissible gastroenteritis virus (TGEV) S protein, for serologic differentiation of pigs infected with TGEV or porcine respiratory coronavirus	./cache/cord-287590-jjft3den.txt	./txt/cord-287590-jjft3den.txt