id author title date pages extension mime words sentences flesch summary cache txt cord-269867-k10siwur Méchin, Marie-Claire The major subunit ClpG of Escherichia coli CS31A fibrillae as an expression vector for different combinations of two TGEV coronavirus epitopes 1996-12-31 .txt text/plain 4118 185 54 Intact bacteria or extracted CS31A hybrid fibrillae expressing TGEV epitopes at any of the permissive sites, were recognized by Ab directed against the foreign parent protein, providing a direct argument for exposure of the corresponding ClpG region at the cell surface and for antigenicity of the epitopes in the polymeric CS31A fibrillae context. coli CS31A fibrillae as the carrier protein (Bousquet et al., 1994; Der Vartanian et al., 1994) , and two B-cell epitopes from TGEV consisting of the site C (aa 363-371) and site A (aa 522-531) of TGEV-S as the foreign antigenic determinants (Delmas et al., 1990; Gebauer et al., 1991) . The binding of the TGEV-specific mAb with hybrid fibrillae indicates that the TGEV antigenic determinants expressed at any of the permissive sites are exposed on both ClpG and CS31A proteins at the E. ./cache/cord-269867-k10siwur.txt ./txt/cord-269867-k10siwur.txt