id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269094-6aka052v	Ortego, Javier	Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway	2007-11-25		.txt	text/plain	6055	318	51	In this article, we confirm that E protein is essential for TGEV replication in different cell lines, and report the first evidence that TGEV virions containing RNA are generated in absence of E protein, although virus maturation was arrested in the budding compartment, and immature virions were accumulated between the rough endoplasmic reticulum and cis-Golgi. To determine the effect of E gene deletion on TGEV infection of BHK-pAPN-E − cells, the subcellular localization of virus particles and endoplasmic reticulum (protein disulfide-isomerase, PDI), intermediate compartment and cis-Golgi (KDEL receptor), and trans-Golgi (giantin) specific markers were compared by immunofluorescence laser scanning confocal microscopy in rTGEV-wt and rTGEV-ΔE infected BHK-pAPN-E − and E + cells at different times post-infection (0, 8, 14, and 24 h post-infection).	./cache/cord-269094-6aka052v.txt	./txt/cord-269094-6aka052v.txt