id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-253041-ts2aiykg	Ding, Li	TGEV nucleocapsid protein induces cell cycle arrest and apoptosis through activation of p53 signaling	2014-03-07		.txt	text/plain	3625	187	51	Moreover, the expression of TGEV N gene promoted translocation of Bax to mitochondria, which in turn caused the release of cytochrome c, followed by activation of caspase-3, resulting in cell apoptosis in the transfected PK-15 cells following cell cycle arrest. Further studies showed that p53 inhibitor attenuated TGEV N protein induced cell cycle arrest at S and G2/M phases and apoptosis through reversing the expression changes of cdc2, cdk2 and cyclin B1 and the translocation changes of Bax and cytochrome c induced by TGEV N protein. Taken together, these results demonstrated that TGEV N protein might play an important role in TGEV infection-induced p53 activation and cell cycle arrest at the S and G2/M phases and apoptosis occurrence. In the present study, we demonstrate that TGEV N protein, when expressed within cells, can cause cell cycle arrest and apoptosis in PK-15 cells, in which p53 and p21 activation, cyclin B1 and cdc2 decrease, Bax translocation to mitochondria, cytochrome c release and subsequent activation of caspase-3 are required.	./cache/cord-253041-ts2aiykg.txt	./txt/cord-253041-ts2aiykg.txt